Home

Xplorer Plus User Manual

1. VB o Bto Jlo and Position a pipette tip under the piston and carefully take the piston off the upper rail Carefully pull the piston out in an upward direction Do not bend the piston Hold the tip cone at the lower end and push it upwards slightly amp This compresses the spring amp Lift the tip cone slightly and release it from the lower rail Relax the spring by letting the tip cone slide back again above the lower rail Remove the tip cone along with the cylinder and spring from the upper rail Before installation slide the piston into the cylinder Install the channels in reverse order 57 32u amp u3 ule A Maintenance 58 Eppendorf Xplorer plus Operating manual Assembling the 10 300 uL multi channel pipette 1 Fit the cover plate with the integrated ejector 2 Push the latches upwards 3 To mount push the lower part into the upper part until it audibly engages Make sure that the magnet on the piston actuator in the lower part is coupled with the magnet on the spindle in the upper part see p 59 4 Carry out a gravimetric test of the systematic and random error see p 63 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly Loosenin
2. Pipette switched on see p 19 Seq Dispensing Spc operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 Press the rocker up to aspirate liquid Press the rocker down to execute the reverse stroke Press the rocker down for each dispensing step If the piston is in the basic position press the Back softkey to exit the mode Bowl Example Required auxiliary aids Xplorer plus with a nominal volume of 300 uL 4 dispensing tubes 200 uL Tube 1 Tube 2 Tube 3 Tube 4 Volume step gt 30 uL 60 uL 90 uL 120 uL Volume step da 120 uL 90 uL 60 uL 30 uL Dilution 14 X 144 141 5 140 67 140 25 Dilution 1 Y 1 5 1 2 5 1 11 67 1 11 25 Tube sum 150 uL 150 uL 150 uL 150 uL 36 Eppendorf Xplorer plus Operating manual 5 13 4 Reverse pipetting Spc dial setting Xplorer plus Only available for the Xplorer plus pipette The Rev Pipetting mode is well suited for plasma serums and other liquids with a high protein content The Pipetting mode is also well suited for aqueous solutions see p 29 The Rev Pipetting mode is also well suited for solutions containing wetting agents in order to minimize the formation of foam during dispensing in the target tube The liquid is aspirated using blow out Blow The blow out is not part of the dispensing volume and may not be dispensed into the target tube If you use t
3. Eppendorf Xplorer plus Operating manual 9 Technical data 9 1 Eppendorf Xplorer single channel Model Test tip Testing Error limits Eppendorf AG Increment epT P S Color code Volume Error enin range Systematic error Random error t tpl t uL 0 5 10 uL medium gray 1 uL 2 5 0 025 1 8 0 018 Increment 0 1 20 L 5 uL 15 0 075 0 8 0 04 all nn 10 uL 1 0 0 1 0 4 0 04 5 100 uL yellow 10 uL t2 0 t0 2 t1 0 0 1 Increment 2 200 uL 50 uL 1 0 0 5 0 3 0 15 llos a tom 100pL 0 8 0 8 0 2 0 2 15 300 uL orange 30 uL 2 5 0 75 0 7 0 21 Increment 15 300 uL 150 uL 1 0 D I 0 3 0 45 0 2 ph am 300uL ng 1 8 0 2 0 6 50 1 000 uL blue 100 uL 13 0 3 0 0 6 0 6 Increment 50 1 000 pL 500 uL 1 0 5 0 0 2 1 THL fimm 1000uL 0 6 6 0 0 2 2 0 25 5mL purple 0 5 mL 3 0 15 0 0 6 3 Increment 0 1 5 mL 2 5 mL 1 2 30 0 0 25 6 25 am om 5 mL 0 6 30 0 0 15 7 5 0 5 10mL turquoise 1mL 3 0 30 0 t 0 60 t6 0 Increment 1 10 mL 5 mL 0 8 40 0 0 20 10 0 EROR 18o mm 10 mL 0 6 60 0 0 15 15 0 d You can select a volume of up to 0 20 mL for the 5 mL Xplorer pipette For the 0 2 mL 0 5 mL volume range handling has a strong influence on the achievable random errors 63 ejep e31uu23 Technical data 64 Eppendorf Xplorer plus Operating manual 9 2 Eppendorf Xpl
4. f 1 2 Remove the piston mount 3 Remove the piston 2 and the piston spring 3 The piston is under spring ST force is applied Carefully pull out the piston and repeat the process correctly In the case of piston springs with double windings 4 these windings must point downwards 2 Keep the piston and the piston spring pressed 3 Keep the stop pins on the piston mount pressed with the other hand 4 Mount the piston mount so that the two locking mechanisms engage in their retainers 5 Gently press a pipette tip against the inserted piston The piston must move down in the cylinder without any noticeable resistance Installing the lower part and ejector sleeve 1 Insert the lower part into the upper part until it audibly engages 2 Setthe maximum volume and move the rocker up and down several times There should not be any unusual running noises 3 Keep the ejector pressed The ejector rod protrudes from the upper part 4 Fitthe ejector sleeve onto the ejector rod If correctly fitted it will engage slightly 5 Carry out a gravimetric test of the systematic and random error This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 32ue amp u3 uielA Maintenance 56 Eppendorf Xplorer plus Operating manual 7 4 2 5 mL and 10
5. E Damage to device from missing pipette tips gt Only use the Xplorer pipette with fitted pipette tips NOTICE Carry over contamination and incorrect dispensing results due to the incorrect use of ate pipette tips NOTICE The pipette tips are for single use only Prolonged use can have a negative impact on dispensing tasks gt Use the pipette tips only once gt Do not use autoclaved ep Dualfilter T I P S for dispensing ae NOTICE Incorrect dispensing volume with special liquids and from temperature differences Solutions which differ greatly from water in terms of their physical data or temperature differences between the pipette pipette tip and liquid can result in incorrect dispensing volumes gt Avoid temperature differences between pipette pipette tip and liquid Check the dispensing volume and make sure that you can affirm all the questions listed in the general information ae NOTICE Damage to device from penetration of liquids Do not deposit the Xplorer pipette with a filled pipette tip Do not allow any liquids to penetrate the inside of the housing gt If liquid has penetrated the inside of the housing have the upper part of the Xplorer pipette repaired only by service partners of Eppendorf AG Contact your responsible sales office before returning a product 3 3 Information on product liability In the following cases the designated protection of the device may
6. In case of warranty claims contact your local Eppendorf contractual partner No warranty is provided for any damage due to misuse or if the upper part has been opened by unauthorized persons The rechargeable lithium polymer battery and all other wear parts are excluded from the warranty Aggressive substances may damage the pipette pipette tips and accessories Check the chemical resistance before using organic solvents or aggressive chemicals gt Observe the cleaning instructions see Cleaning on p 51 Components of the Xplorer pipette that can be accessed by the user are made of the following materials Component Material External surfaces of the upper part Improved polypropylene PP Polycarbonate PC Polycarbonate PC coated Polycarbonate PC dyed Gold plated Exterior and interior of lower parts Improved polypropylene PP Polyvinylidene fluoride PVDF Polyetherimide PEI Polyphenylene sulfide PPS Polyetheretherketone PEEK Polytetrafluoroethylene PTFE Ethylene propylene diene rubber EPDM Silicone Steel stainless steel and spring steel Pipette tip Material epT I P S Polypropylene PP epDualfilter T I P S filter Polyethylene PE 13 uonduosap jonpo4d ka Safety Eppendorf Xplorer plus Operating manual 3 Safety 3 1 Intended use The Xplorer pipette is a lab device intended for dispensing liquids in
7. Mean value of the weighings Actual volume L Density liquids at weighing temperature The density of distilled water is Approx 0 9982 mg uL at 20 C and 0 9965 mg uL at 27 C If the set volume corresponds to the actual volume no correction is necessary If there is a difference between the actual volume and the set volume of distilled water please check the following s there any liquid dripping from the tip s the pipette tip fitted leak proof Adequate leak tightness is ensured when no drop is formed at the pipette tip after aspiration of the nominal volume with distilled water and a waiting time of approx 15 s Hold the pipette vertically without touching the pipette tip Prewet the pipette tip several times s the tip cone undamaged Are the piston and the cylinder leak proof 61 jusunsn pe uo uorjeuuojur e4dUdH General information on adjustment 62 Eppendorf Xplorer plus Operating manual Does the temperature of the pipetted liquid correspond to The temperature of the device The ambient air temperature s the weighing location free from drafts Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid Has the correct numerical value for Density liquids at weighing temperature been used for the calculation of the actual volume s the set volume correct For very small volumes 10 pL is
8. there is a risk of the liquid coming into contact with the dualfilter because of the additional volumes during aspiration For pipette tips or filter tips whose aspiration volume is smaller than the nominal volume of the Xplorer pipette ep Dualfilter T I P S Volume limit Ads Dis Reverse pipetting 10 uL Not required Not required 100 uL Not required Not required 200 uL Not required Not required 300 ul 270 uL 260 uL 1 000 uL Not required 950 uL 1 250 uL Long Not required 1 130 uL 5 mL 4 9 mL 4 6 mL 10 mL 9 75 mL 8 5 mL When using these filter tips we recommend using the ep7 PS long adjustment to increase the accuracy of dispensing The specified volume limit only applies to the changed adjustment We also recommend this volume limit for Reverse Pipetting when using 5 mL and 10 mL epT P S without filter Other volume limits may apply when using filter tips from other manufacturers The volume limit differs depending on the tip geometry and the position of the filter in the tip The volume limit is dependent on the additional aspirated volumes of the Xplorer pipette On the Xplorer the individual nominal volumes result in different additional volumes 43 uone13d o Operation 44 Eppendorf Xplorer plus Operating manual 5 17 3 Counter 5 17 4 Ejector reset Xplorer Additional aspiration volumes Single chann
9. 12 tips can also be mounted You can open the multi channel lower part in order to exchange or remove individual channels 3 Latches right and left Unlock the cover plate 6 and internal ejector rail The 1 200 uL multi channel lower part can be opened with a pin see p 58 4 Spring loaded tip cones for 10 uL 100 uL and 300 uL The spring loading action optimizes the force required for attaching and ejecting tips 5 Pipette tips We recommend using epT P S 6 Cover plate for 10 uL 100 uL and 300 uL Removable cover plate with internal ejector rail The cover plate is opposite the side with the lever 1 2 2 Display The brightness of the display can be changed in the options see p 42 The display dims during usage intervals and switches off after a prolonged usage interval Move the Xplorer pipette to switch the display back on WTA Pipetting A 500 4 Speed Counter Ox 1 Header 2 Status line 3 Main field 4 Footer Eppendorf Xplorer plus Operating manual 2 2 1 Header Adjustment O The adjustment can be changed in the options The adjustment is described separately see p 61 Detailed information can be found in a separate document on the CD and on our website www eppendorf com 4 If you have changed the factory settings the symbol will be displayed to the left of the header Additional symbols to the right indicate which adj
10. C CE Ce CE Te EEE e Ce E ct et d H pri c yr T C T3 C Hi A e c amp T C C C gt C et ce r amp ce TuS ce ce C ce Cs ce e LE re LE gt A r Z i J Ope Y ng Manual j Y Y Evaluate your operating manual www eppendorf com manualfeedback eppendorf In touch with life Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com
11. Spring loading action is blocked by a locking ring Remove the locking ring again Use a 5 mL or 10 mL single channel pipette or 1 200 uL multi channel pipette No remedy The tip cone of these sizes do not have spring loading action Battery state Z Battery empty Confirm with OK Connect power supply immediately The rechargeable battery is heavily discharged Confirm the message with the OK softkey Complete the dispensing operation as quickly as possible and charge the rechargeable battery for 3 hours You can continue dispensing with the power supply connected Then charge for an additional 3 hours ec The rechargeable battery is almost The rechargeable battery must be charged Battery state completely discharged The remaining for at least 15 minutes before further use rechargeable battery capacity is only A charging time of 3 hours is a Battery empty e 4 d h Usage not sufficient for displaying the information to recommended possible 3 hours the left Dispensing cannot performed charging time The following message appears briefly before the aspiration Selected volume not possible with stored adjustment A factor that was determined during the Xplorer adjustment leads to a very high stroke increase This high stroke increase limits the dispensing volume gt Confirm the error message with the OK softkey The situation can only be remedied by changing the adjustment Make s
12. be compromised Liability for any resulting property damage or personal injury is then transferred to the operator The device is not used in accordance with the operating manual The device is used outside of its intended use The device is used with accessories or consumables which are not recommended by Eppendorf The device is maintained or repaired by people not authorized by Eppendorf The user makes unauthorized changes to the device 15 Ayajes Installation Eppendorf Xplorer plus Operating manual 4 Installation 4 1 Power supply assembly 1 Power plug adapters a EU b United Kingdom c USA d Australia 2 Power supply 3 Charging plug 3 2 gt Fit the power plug adapter required for your power supply into the opening of the power supply In the event of any doubt which power plug adapter should be used for the power supply unit you should ask an electrician for advice 4 2 Connect rechargeable lithium polymer battery The lithium polymer battery is already inserted in the battery compartment upon delivery 1 Openthe rechargeable battery compartment cover 2 Connect the plug of the rechargeable lithium polymer battery to the connector socket 3 Closethe rechargeable battery compartment cover On the Xplorer plus pipette you will then be prompted to set the date and time see p 19 Eppendorf Xplorer plus Operating manual 4
13. lower part automatically snaps into the upper part on insertion Make sure that the magnet on the piston actuator in the lower part is coupled with the magnet on the spindle in the upper part 8 Carry out a gravimetric test of the systematic and random error see p 63 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly We recommend that you have all maintenance and servicing carried out by Eppendorf AG or Eppendorf service partners No warranty is provided for any damage due to misuse or if the device has been opened by unauthorized persons Eppendorf Xplorer plus Operating manual 8 General information on adjustment NOTICE Incorrect dispensing volume with special liquids and from temperature differences Solutions which differ greatly from water in terms of their physical data or temperature differences between the pipette pipette tip and liquid can result in incorrect dispensing volumes gt Avoid temperature differences between pipette pipette tip and liquid Check the dispensing volume and make sure that you can affirm all the questions listed in the general information The random and systematic errors determined before delivery can be found in the enclosed Eppendorf Certificate If the factory settings are superseded by a different adjustment the Eppendorf Certificate is no longer valid If you reset the adjustment to the factory setti
14. mL single channel The piston must be in its basic position before separating the lower and upper part Press the rocker down to move the piston to its basic position 1 O Unscrew the ejector sleeve 2 Keep the ejector pressed and turn the lower part to the left or right by approximately 30 Pull the lower part down to cancel the magnetic coupling of the lower part The lower part is separated from the upper part 3 Open the lower part fit the pipette key included in the delivery package onto the lower part Hold the cylinder and unscrew it from the lower part The oylinder with the tip cone is separated from the upper part of the lower part The upper part of the lower part and the piston cannot be disassembled any further See the next figure The lower part consists of the following 1 Upper part of the lower part 2 Piston with seal Piston rod with magnet covered here by 1 Upper part of the lower part 3 Cylinder and tip cone 1 E Co The assembly is performed in reverse order Make sure that the magnet on the piston in the lower part is coupled with the magnet on the spindle in the upper part After the assembly Carry out a gravimetric test of the systematic and random error This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly For replacing an ejector sleeve the 5 mL and 10 mL sizes are delivered wi
15. mx kW 47 9 17 12 SONICO EE 48 5 17 13 Service interval ooo en 48 9 17 14 Date and TIMO sais de dee STE ee pr 48 6 Troubleshooting nennen nennen 49 6 1 RESET ues eov da 49 6 2 A Hirst EP E M Ets dini A mtn DARUM M dA S EN 49 7 Malnten ance u su 0000 a a a AAA 51 7 1 Gleaningi s oe eet es bob e bee eto d Ganda a a dtd eb beet d erede da 51 7 2 Sterilizing or disinfecting the pipette liliis RII II It 51 7 2 1 Autoclaving ser eve E epo S ues eR eg eem pi dpa den gx dde 52 7 2 2 Disinfecti n cur PREMO ERE a REI 52 7 3 Beplacing O fings ool Heer eel PEU bL EI ehe Zei A 52 7 3 1 Removing tlie Osring stunt a Ud ERR e AN e RUE Ie RE a 53 7 3 2 Mounting a new Omg 53 7 4 Xplorer pipette disassembly and assembly 00 00 cece ee en 53 7 4 1 Single channel up to 1 OOO UL ee m 53 7 4 2 5 mL and 10 mL single channel e nh 56 7 4 8 M ltischarinel z ver RR RR exor Y Ee Eee PEE KE dap E ere 57 7 9 Maintenance creber RN DE S REX MENS RR ERES RN EXE RR RN E Ben 60 8 General information on adjustment n n n hh hh 61 9 Technical data ociosa RER nx aar dee NR ENEE EE A ne REA Rer 63 9 1 Eppendorf Xplorer single channel 63 9 2 Eppendorf Xplorer multi channel liiseleeseeeee nennen een rums 64 9 9 Ce ul tte IEN 65 9 4 Speed table for Xplorer single channel llle mnn 66 9 5 Speed table for Xplorer multi channel 2 00 00 cece eee n 66 Eppendorf Xplorer plus Operating m
16. now usually given in Pa s or mPa s The unit P or cP is frequently found in older literature 1 mPa s corresponds to 1 ch At room temperature a 50 glycerin solution has a viscosity of approx 6 mPa s As the glycerin concentration increases viscosity increases considerably Absolutely anhydrous glycerin has a viscosity of approx 1480 mPa s at room temperature Liquid dispensing during which the pipette tip touches the tube inner wall We recommend completing a dispensing series using only the free jet method or wall dispensing method Eppendorf Xplorer plus Operating manual 2 Product description 2 1 Main illustration Single channel Pipeliing Sues 51000 d Counts Om 13 14 10 11 12 13 14 USB port Only for Eppendorf service ON switch Reset key Connector socket For connecting the charging plug of an Eppendorf Xplorer power supply Selection dial For setting an operating mode Charging contacts Gold plated contacts for loading and storage in a series 4880 charger stand or charger carousel Backlit color display Switch the display off when it is not in use The display automatically switches back on when the pipette is moved Softkeys The assignment of the respective softkey is shown in the display footer Rocker You can either press the rocker up or down The rocker can either be us
17. the 10 uL 100 uL and 300 uL multi channel lower parts by installing 2 3 locking clips The locking clips are part of the delivery package Requirement Multi channel lower part is open and has been removed from the upper part see p 57 Channels removed see p 57 1 Remove the spring from the channels 2 Insert the channels without the springs N NU nw eee eU ch 3 Firmly press the locking clips onto the channels 4 Assemble the pipette see p 58 Eppendorf Xplorer plus Operating manual Opening and assembling the lower part x1 000 pL O To avoid confusion of parts only disassemble and assemble one pipette at a time The piston must be in its basic position before separating the lower and upper part If necessary press the rocker down to move the piston to its basic position Opening the lower part Push the stop pins together slightly on the piston mount 1 1 2 tension The appearance of pistons and piston springs will vary for the 3 individual volume versions Assembling the lower part 1 Carefully guide the piston and the piston spring into the cylinder Make sure that the piston is guided correctly in the piston spring and the cylinder There must not be any perceptible resistance Stop pushing as soon as E 4 any resistance is felt This may indicate that the piston is not positioned i correctly in the cylinder There is a risk of bending the piston if too much
18. the rocker Press the End softkey to save the changes to exit the edit mode and to continue with dispensing Pons O If you want to cancel editing without saving the changes made briefly turn the selection dial to another mode O You can change the Speed Time Cycles and Counter parameters during the dispensing Press the Edit softkey during the execution Set volume Pipetting Aspiration and dispensing volume Set volume You can change the speed that the Xplorer pipette uses to change the A 382 uL volume under the Hocker setting parameter in the options Next p End Set max volume EE Maximum aspiration and dispensing volume Manual pipetting The set value appears after editing in the display next to Max A 0 uL Speed mex 1000pL Edit ep Help Set volume step Dispensing Volume per dispensing step The maximum number of aspiration Set volume step steps Steps will be displayed when the Set volume step is changed A HL Aspiration speed Aspiration speed Aspiration speed 8 levels are available The higher the numerical value the higher the AS speed Speed Aspiration speed will be automatically and approximately used for the blow out Blow Next ep End Eppendorf Xplorer plus Operating manual Dispensing speed Dispensing speed 8 levels are available The higher the numerical value the higher the speed Speed Dispensing speed will be automatic
19. you turn the selection dial to the Spc dial setting the special operating modes will be shown in a selection list in the display 5 13 1 Multiple aspiration Aspirate Spc dial setting Xplorer plus Only available for the Xplorer plus pipette A specific volume will be aspirated in the Multi Aspirate mode several times All of the liquid will be dispensed after the aspiration The Multi Aspirate mode is the opposite of the dispensing mode P Bl d 3 U v Aspirating partial volumes Dispensing Requirement Pipette switched on see p 19 e Multi Aspirate Spc operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 O The Set volume step parameter determines the volume to be aspirated for each step The maximum number of aspiration steps Steps will be displayed when the Set volume step is set 1 For every aspiration press the rocker up to aspirate liquid The following message appears in the display after the aspiration has been completed Piston is in top position Press rocker down 2 Press the rocker down to dispense all of the liquid 3 Press the rocker down again to trigger the blow out Blow 4 If the piston is back in the basic position press the Back softkey to exit the mode uone13d o Operation Eppendorf Xplorer plus Operating manual 5 13 2 Diluting Spc dial setting Xplorer plus Only available for t
20. 0 and General information about adjustment see p 61 The following adjustments can be selected Factory settings Resets the adjustment to the factory setting Liquid type Ethanol 75 The factory setting is changed by a factor in order to allow 75 ethanol to be dispensed with higher accuracy The internally used factor takes into account the density and the following work technique in the Pip mode 1 Wet the tip with liquid three times at room temperature Hold the Xplorer pipette in an upright position Avoid outer wetting during dispensing 2 Use speed level 5 standard setting for aspiration and dispensing 3 Dispense liquid against the tube wall of the destination tube 4 Trigger a blow out Blow approx 2 seconds after dispensing Then eject the tip Liquid type Glycerol 50 The factory setting is changed by a factor in order to allow 5096 glycerol to be dispensed with higher accuracy The internally used factor takes into account the density of the 50 glycerol and the same work technique as described previously for Ethanol 75 in the Pip mode epTIPS long In addition to the standard tips a second longer tip is available for most Xplorer pipettes When epTIPS long is selected the tip geometry of this longer tip is taken into account in the internal volume calculation This reduces the systematic error of dispensing when using these tips 45 uone13d o Operation 46 Eppendorf Xplorer plus Operati
21. 1 Pipetting and mixing P M dial seng 31 5 12 Manual pipetting Man dial seng 32 5 13 Special operating modes o oococo eere 33 5 13 1 Multiple aspiration Aspirate Spc dial seng 33 U3JUO JO 3 81 Table of contents Eppendorf Xplorer plus Operating manual 5 13 2 Diluting Spc dial seng 34 5 13 3 Sequential dispensing Spc dial seng 36 5 13 4 Reverse pipetting Spc dial setting llle 37 5 13 5 Sequential pipetting Spc dial seng 38 5 14 Program Prg dial seng 39 5 15 Editing Edit dial seng 39 5151 Pswrd Protection nsi AV a waa en RENE De ERR AGE 40 5 15 2 Fixed volume sisie caiaeii de bd tod Lee pee ER P RP e mL eene s 40 9 15 39 Programm nous kk ERE Are See REESE ERRARE wees 41 5 16 Pipetting with fixed volume Fix dial eeng 42 5 17 Option Opt dial setting oooooococrococoora HR mr 42 547 General ely nolle RISE ERR deed eben Debe lob bated Hd e tede 42 5 72 Volume liM uii LR a E RURIA RA es 43 SS ee TEE 44 547 4 BEjectorresel xXx AR Ro SERE URGE RD BN CU PR PE REN E 44 5 17 5 y LE O y EE 44 9 17 6 Rockerselllg ss nr ee een 44 5477 So nd eVel icto tede ex diye nee ne e Bd TVA rose da ale ee De 44 5178 Brightiess io ra Ere haa T eR eee aaa 44 5 17 9 Adjustment sioari D ER bd ca rd re E A tt ed hdd ted ood a 45 9 17 10 Language ck Sr aa e nd e e Ree NEEN d 47 5 17 11 Personalizatlon zoe EH EE a A A A UA RE ERE eurem
22. 1 2 1 2 2 8 2 8 8 0 9 0 9 0 9 0 9 2 6 2 6 You can select a volume of up to 0 20 mL for the 5 mL Xplorer pipette For the 0 2 mL 0 5 mL volume range handling has a strong influence on the achievable random errors 9 5 Speed table for Xplorer multi channel The following speed table applies to Xplorer pipettes starting with software version 2 01 00 Speed level 10 pL 100 uL 300 pl 1200 pL Speed Time sec Time sec Time sec Time sec 1 12 0 12 0 12 0 12 0 2 8 0 8 0 8 0 8 0 3 4 0 4 0 4 0 4 0 4 2 8 2 8 2 8 2 8 5 2 2 2 2 2 2 2 2 6 1 6 1 6 1 6 1 6 7 1 2 1 2 1 2 1 2 8 0 9 0 9 0 9 1 0 Eppendorf Xplorer plus Operating manual 10 Ordering Information gt f The ordering information can be found on the CD for the Xplorer pipette o 67 uoneuuoju Buap Transport storage and disposal Eppendorf Xplorer plus Operating manual 11 Transport storage and disposal 11 1 Decontamination before shipment If you wish to return the pipette to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired please note the following Use of a contaminated device may result in personal injuries and damage to the device IN Clean and decontaminate the Xplorer pipette before shipping or storage according to the 4 2 cleaning instructions CAUTION Hazardous substances are solutions present
23. 3 Charging the rechargeable battery WARNING NOTICE Incorrect or damaged power supplies can lead to severe person injury or damage to the device Incorrect or damaged power supplies can cause electric shock or cause the device to overheat catch fire melt short circuit or cause similar damage gt Only use the supplied power supply for charging the device The correct power supply unit is indicated via the Eppendorf logo and the device name on the power supply Donotuse a damaged power supply Loss of full rechargeable battery charging capacity if charged incorrectly The supplied rechargeable battery is not charged completely If the rechargeable battery is not charged completely before it is used for the first time its charging capacity will remain permanently greatly reduced The rechargeable battery will only achieve its full capacity after a few discharge charge cycles Charge the rechargeable battery for at least three hours before it is used for the first time gt Do not charge the rechargeable battery in a hot location 260 C Only charge the rechargeable battery using the supplied power supply For identification purposes the power supply contains a sticker with the name of the compatible devices The power supply is designed for the 100 V 240 V range eppendorf 4986 603 005 Multipette stream Xstream Repeater stream Xstream Xplorer Recharging the rechargeable battery 1 Insert the as
24. Capacity 750 mAh 3 7 V Charging time Approx 3 hours Number of dispensing operations Approx 1400 in the Pip mode with fully charged battery and average dispensing speed with a single channel version lt 1 000 uL in continuous operation Weight Approx 20 g Power supply Type Power supply for Xplorer Multipette Repeater stream Xstream with plug in power plug adapters Input voltage 100 V to 240 V 10 50 60 Hz Output voltage 5V 1A Xplorer Operating temperature 5 to 40 C Air humidity during operation 10 to 95 rel humidity Storage temperature 5 to 45 C Air humidity during storage 10 to 9595 rel humidity Weight without rechargeable battery without pipette tip 100 uL single channel approx 135 g 0 30 Ib 100 uL eight channel approx 213 g 0 47 Ib 65 ejep e31uu29 Technical data 66 Eppendorf Xplorer plus Operating manual 9 4 Speed table for Xplorer single channel d The following speed table applies to Xplorer pipettes starting with software version 2 01 00 Speed 10 pL 100 uL 300 pl 1 000 ul 5mL 10 mL level Speed Time sec Time sec Time sec Time sec Time sec Time sec 1 12 0 12 0 12 0 12 0 12 0 12 0 2 8 0 8 0 8 0 8 0 8 0 8 0 3 4 0 4 0 4 0 4 0 6 0 6 0 4 2 8 2 8 2 8 2 8 4 8 4 8 5 2 2 2 2 2 2 2 2 3 6 3 6 6 1 6 1 6 1 6 1 6 3 2 3 2 7 1 2 1 2
25. Eppendorf Xplorer plus Operating manual 5 9 Dispensing Dis dial setting In the Dispensing Dis mode liquid is aspirated and individually dispensed in uniform partial volumes The reverse stroke and remaining stroke are triggered before or after the dispensing steps These additional volumes may result in volume limits when using filter tips see p 43 Bi Aspiration Reverse stroke Dispensing Aspirate or empty Requirement Pipette switched on see p 19 Dispensing Dis operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 Press the rocker up to aspirate liquid 2 Press the rocker down to trigger the reverse stroke 3 Press the rocker down again for each dispensing operation The display indicates the remaining dispensing steps under Steps After dispensing decide whether the residual liquid should be dispensed or liquid should be aspirated again gt Liquid aspiration press the rocker up once gt Liquid dispensing press the rocker down twice O Press the Empty softkey to cancel dispensing The tip is completely emptied Press the rocker up to stop a current dispensing of a large dispensing volume O Free jet dispensing may be used to dispense aqueous solutions with a volume of approximately 20 uL or more We recommend wall dispensing for dispensing aqueous solutions with a smaller volume Select level 8
26. PhusioCare Sy Eppendorf Xplorer Eppendorf Xplorer plus Operating manual eppendorf Copyright 2012 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner eppendorf epT I P S 9 and Eppendorf Xplorer are registered trademarks of Eppendorf AG Hamburg Germany Trademarks are not marked in all cases with Mor Gin this manual Xplorer pipettes are using U S Patent Nos 7 694 592 B2 8 028 592 B2 7 674 432 B2 7 585 468 B2 7 434 484 B2 6 778 917 6 499 365 Further information can be found on the enclosed CD or on our website www eppendorf com 4861 910 011 00 022012 Eppendorf Xplorer plus Operating manual Table of contents Operating instructions sss lees back Pe EER ee Oe eee eee 6 1 1 Using this manual 3o nee ha de did er bebe m eie nbl dete ee 6 1 2 Danger symbols and danger levels o ooococcoocoo nern ren 6 1 2 1 Hazard ICONS e c 6 1 2 2 Degrees of danger esci RE wage a aan Pr A AUR RUNE MUR EUR XN BOR A CR 6 1 3 Symbols TE EE 6 TA Glossaty i eso puer a ENEE dn KEE A dee rore de eau uxo I E pe Pass sug VER 7 Product description 00 cece cece eee eee en nennen hh hanh e nnn nnn 9 2 1 Mainillustr tion oer reme pm ee des ia eoe ADR PS Re re poe doe PR ee et 9 2 1 1 Single chantiel rrr Rame terme mos heal alge ORO E PCR Bp dE 9 2 1 2 Multi channel 22 da REA E at wd d
27. The Xplorer plus pipette will be reset to the factory settings O If you lose the password for the Edit mode you will not be able to change any programs or pipettings with fixed volume 1 Press the rocker upward to activate the password protection ON appears in the display Press the Select softkey Use the rocker to select the digit Press the Next softkey to switch to the next field Enter data in all of the fields Press the Save softkey to save the password nog bah Reenter the password after completing the saving procedure Press the Enter softkey Password protection is now active 5 15 2 Fixed volume Creating a new pipetting 1 Select the New fixed volume entry using the rocker The parameters of the most recently used pipetting will be displayed when the New fixed volume entry is opened Up to 10 fixed volumes can be saved 2 Press the Edit softkey 3 Change the parameters as you did in the Pipetting mode 4 Press the Save softkey The saved pipetting now appears in the Fixed volumes Fix operating mode with dispensing volume aspiration speed and dispensing speed see p 42 Changing saved pipettings 1 Select the pipetting using the rocker 2 Press the Select softkey 3 Select the Edit option 4 Press the Select softkey Eppendorf Xplorer plus Operating manual 5 15 3 Programm Creating a new program 1 Select the New program entry using the rocker Up to 10 programs can be saved You wil
28. a single step Dispense the liquid against the tube inner wall if you are determining the systematic and random errors If you only hold the rocker pressed down during the blow out Blow after dispensing the piston remains in the lowest position The piston will only return to the basic position after the rocker has been released This presents the following advantages gt If you pipette liquid that drains slowly you can allow this liquid to flow out You can immerse the pipette tip in the target liquid to prevent unintentional aspiration gt You can trigger the blow out Blow any time the piston is in the basic position Press the rocker down Press the rocker in the opposite direction if you would like to stop aspiration or dispensing while it is in progress The display shows the volume present in the pipette tip after the respective operation has been stopped You can then continue with liquid aspiration or dispensing gt The number of dispensing steps performed can be counted with a counter Counter The counter can be activated Counter if desired see p 42 29 uone13d o Operation 30 Eppendorf Xplorer plus Operating manual 5 10 2 Reverse pipetting Reverse pipetting is advantageous for plasma serum and other liquids with a high protein content Reverse pipetting is not necessary for pipetting aqueous solutions By using the blow out more liquid is aspirated with reverse pipett
29. ally and approximately used for 6 the blow out Blow Set time Auto dispensing Y Interval between the dispensing step 0 1 s to 10 s Set time Time Next ep End Number of steps Dispensing 4 Bf Number of dispensing steps Number of steps The available number of dispensing steps depends on the setting for A Set volume step and is automatically set to the maximum number Steps when Set volume step is edited Under steps 14 Sin the Sequential dispensing mode the selected MS number of dispensing steps will be displayed to the right and the dispensing step for the displayed volume will be shown at the left Set counter SEE The counter counts the number of dispensing operations performed Set counter The numerical value of the counter is increased by 1 when the piston A reaches the basic position after dispensing Counter If the piston is in the basic position you can alternatively set the counter to 0 by turning the selection dial forward or backward Next ep EE You can switch the counter on and off in the Options Mixing volume Pipetting Mix a The mixing volume used after pipetting If you change the pipetting Mixing volume volume under Set volume the mixing volume changes accordingly A Mix 1000uL Next ep End Mixing cycles Pipettinge Mix EH 1 to 99 mixing cycles can be set for the mixing volume The mixing Mixing cycles cycles are counted down to 0 If you keep the rocker pre
30. and leak tightness Biopur products also meet the highest standards when it comes to sterility and being free of ATP PCR inhibitors human and bacterial DNA pyrogens DNase and RNase Goods with the Biopur purity level are inspected and certified by an external laboratory Lot specific certificates can be downloaded from our website www eppendorf com Movement of the piston that dispenses the remaining liquid in the pipette tip after the pipetting step has been completed When using normal pipetting the liquid blow out is part of the dispensing volume When using reverse pipetting blow out is not part of the dispensing volume C Together the piston movement upward liquid aspiration and piston movement downward liquid dispensing form a cycle Dispensing steps without a pipette tip touching tube wall If a drip forms on the pipette tip after free jet dispensing this drip always belongs to the next dispensing step We recommend completing a dispensing series using only the free jet method or wall dispensing method Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method Errors listed in the technical data were determined using the wall dispensing method Step size or resolution For example the volume can be increased or decreased in 0 01 mL increments for the volume selection on a 10 mL Xplorer pipette The ISO 8655 standard defines among other factors limiting va
31. annel up to 1 000 pL Removing the lower part 1 Keep the ejector pressed and remove the ejector sleeve 2 and On the lower part slide the ring marked PUSH UP TO RELEASE up by about 5 mm until the lower part comes off 3 Remove the lower part from the upper part 53 32u amp u3 uIelA Maintenance 54 Eppendorf Xplorer plus Operating manual Deactivating spring loading For the following dispensing tasks it may be helpful to deactivate the spring loading action of the tip cone A pipette tip is to be used for a long period The pipette tip is slightly bent during dispensing Deactivating this action may also be helpful for pipette tips that are not designed for the Eppendorf pipette tip cone Single channel pipettes The spring loading action of the tip cone can be deactivated by installing the locking ring The locking ring is included in the delivery package 1 Push the black locking ring onto the lower part from the top by slightly squeezing the clamps on the lower part 2 Insert the lower part into the upper part until it audibly engages 3 Keep the ejector pressed The ejector rod protrudes from the upper part 4 Fitthe ejector sleeve onto the ejector rod If correctly fitted it will engage slightly Remove the locking ring to reactivate the spring loading action Multi channel pipettes The spring loading of the tip cones can be deactivated in
32. anual 10 Ordering Information eI RR t x e em Run RR RR ORE Re REG RR EELER nn 67 11 Transport storage and disposal oooooccccccccn ee nh hh hh hh nnn 68 11 1 Decontamination before shipment o cococcccococ m ms 68 aer TT 68 11 9 DISPOSAL EE 69 lp 4r 70 sju3 uoo JO 3 81 Operating instructions Eppendorf Xplorer plus Operating manual 1 Operating instructions 1 1 1 2 1 2 1 1 2 2 1 3 Using this manual Read this operating manual completely before using the device for the first time Also observe the instructions for use of the accessories gt This operating manual is part of the product thus must always be easily accessible gt Enclose this operating manual when transferring the device to third parties gt If this manual is lost please request another one The current version is available on our website www eppendorf com gt This operating manual applies to devices with software version 2 01 00 or higher The operating manual in additional languages adjusting instructions and additional information can be found on the Xplorer CD or on our website www eppendorf com Danger symbols and danger levels Hazard icons Biohazard Explosion Electric shock Toxic substances Hazard point EN Material damage Ne Degrees of danger The safety instructions in this operating manual indicate the following degrees o
33. bility and check the manufacturer s instructions about chemical resistance Consider also the material of the pipette 51 32u amp u3 uIelA Maintenance Eppendorf Xplorer plus Operating manual 7 2 1 Autoclaving All Xplorer lower parts are steam autoclavable Before autoclaving 1 Remove any contamination from the exterior and in the lower part see Xplorer pipette disassembly and assembly on p 53 2 If you remove grease only relubricate the piston seal slightly with the grease specified in the ordering information see the Xplorer CD Procedure Follow the operating manual of the autoclave manufacturer 1 Autoclave at 121 C 20 minutes 1 bar pressure 2 Putthe lower part into the autoclave in such a way to ensure that the temperature at the lower part does not exceed 121 C 3 You can put the lower part into the autoclave as a whole unit or as individual parts 4 Make sure that no parts of disassembled lower parts are confused during subsequent assembly recommendation use a plastic beaker for each lower part After autoclaving 1 Cool the lower part down to room temperature and leave to dry The piston does not need to be greased following autoclaving 2 Carry out a gravimetric test to check that the Xplorer pipette is functioning 7 2 2 Disinfection 1 Carefully wipe off the outer surfaces with disinfectant DNA RNA decontamination agents or 7096 isopropanol 2 You can wipe the outside of th
34. de N Turn the selection dial to an operating mode to exit the options and to continue with dispensing operations O Press the Help softkey to display information on the selected option 5 17 1 General help This describes the elementary steps for all modes This option does not contain any adjustable parameters The help texts in the Xplorer are not a substitute for reading the operating manual 42 Eppendorf Xplorer plus Operating manual 5 17 2 Volume limit This limits the aspiration volume in all dispensing modes d After a volume limit has been set a note will be shown in the display when the Pip P M and Man operating modes are edited and when the volume limit has been reached In this mode too large dispensing volumes will be automatically reduced to the defined volume limit The number of potential dispensing steps will be automatically limited in the Dis and Ads modes If the corresponding volume is above the defined volume limit saved dispensing can no longer be executed under History and under the Spc Prg and Fix modes The volume can be changed in the corresponding mode or the Edit mode Use the volume limit in the following cases For strongly foaming solutions in order to prevent contamination of the tip cone or the protection filter e When using ep Dualfilter T I P S in the Ads Dis and Pip modes reverse pipetting and the Xplorer plus Rev Pipetting mode For these three versions
35. e Auto dispensing Ads mode aspirate liquid and dispense it in uniform partial volumes in selected time intervals The reverse stroke and remaining stroke are triggered before or after the dispensing steps These additional volumes may result in volume limits when using filter tips see p 43 S Bi Bi Fa laf lo lll Lf Aspiration Reverse stroke Auto dispensing Aspirate or empty Requirement Pipette switched on see p 19 Auto dispensing Ads operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 Press the rocker up to aspirate liquid Then follow the instructions in the display 2 Press the rocker down to trigger the reverse stroke 3 Keep the rocker pressed for the duration of dispensing After the first dispensing step the following dispensing steps are triggered with the displayed time interval Time 0 gt Press the Empty softkey to cancel dispensing The tip is completely emptied When you release the rocker an ongoing dispensing operation is still being completed The display shows the possible dispensings under Steps Press the rocker again to continue dispensing After dispensing decide whether the residual liquid should be dispensed or liquid should be aspirated again gt Liquid aspiration press the rocker up once gt Liquid dispensing press the rocker down twice 27 uone13d o Operation
36. e rocker e e is held pressed down Opt Options Making settings and changing the e e adjustment Spc Multiple aspiration Aspiration of uniform partial volumes and e combined dispensing reverse dispensing Diluting For diluting a sample or reagent Sequential dispensing e Functions like dispensing mode with a fixed sequence of various volumes Reverse pipetting e Functions like the pipetting mode but in reverse order Sequential pipetting e For creating pipetting series with various volumes Prg Program e Execute saved dispensings Edit Editing e Procedure programs and pipettings with fixed volumes Fix Pipetting with fixed volumes e Procedure pipettings with fixed volumes gt To select an operating mode turn the selection dial to the selected mode uone13d o Operation 22 Eppendorf Xplorer plus Operating manual Xplorer plus Only available for the Xplorer plus pipette If you have selected the Spc operating mode 1 Use the rocker to select the required operating mode from the list 2 Press the Select softkey 5 5 Setting the parameter edit mode Requirement Pipette switched on see p 19 Operating mode selected see p 21 Change the parameters as follows Press the Edit softkey to open the edit mode Press the Next softkey to go to the next dispensing parameter Change the dispensing parameter marked in the display with
37. e tip cone and the ejector sleeve with a sodium hypochlorite solution of 4 3 After the exposure time of the sodium hypochlorite solution has elapsed thoroughly remove it using demineralized water 7 3 Replacing O rings The 100 uL 300 uL and 1 200 uL multi channel lower parts are equipped with O rings These are wear parts Replace old worn or damaged O rings Defective O rings result in the incorrect positioning of the tips and in dispensing errors Eppendorf Xplorer plus Operating manual 7 89 4 Removing the O ring 1 Push the opening of the O ring tool included in the delivery package against the tip cone from the side so that the sharp edge in the opening of the O ring tool is positioned on top of the o ring Do not slide the O ring tool onto the cone The figure shows the O ring tool for 100 uL and 300 pL A separate O ring tool is available for 1 200 uL 2 Supporting the O ring tool with your thumb push it firmly against the tip cone This O ring is cut in one place 3 Remove the O ring tool and the O ring from the tip cone 7 3 2 Mounting a new O ring 1 Fit the mounting aid shortened pipette tip included in the delivery package of the O rings on the tip cone 2 Push the new O ring over the tip onto the tip cone 3 Check that the pipette tips are correctly positioned Ensure that the tips are tightly sealed and properly aligned 7 4 Xplorer pipette disassembly and assembly 7 4 1 Single ch
38. ed to trigger the piston movement or to scroll through the menu and change the values of parameters The color of the rocker is different for the various volumes and matches the color of the trays for the suitable epT I P S pipette tips Ejector Moves the ejector sleeve and ejects the pipette tip After a filled pipette tip has been ejected the piston returns to the basic position after a slight delay This option can be switched off see p 42 Serial number Ejector sleeve The ejector sleeve is marked with the nominal volume of the pipette Spring loaded tip cone The spring loading action optimizes the force required for attaching and ejecting tips The spring loading action is available for all Xplorer pipettes up to 1 000 uL Pipette tip The Xplorer pipettes may only be used with the suitable pipette tip fitted We recommend using epT P S uonduosap j1onpo4d Product description Eppendorf Xplorer plus Operating manual 2 1 2 Multi channel A description of the upper part can be found in the main illustration of the single channel pipette see p 9 1 Lever The lever is used to release the multi channel lower part 2 Multi channel lower part The multi channel lower part is autoclavable The multi channel lower part is freely rotatable It is not detached when rotated The outer channels are marked with the numbers 1 and 8 or 12 The multi channel version has a piston for each channel so that less than 8 or
39. el and Ads Dis Reverse pipetting multi channel 10 uL Approx 0 6 uL Approx 2 uL 100 uL Approx 7 uL Approx 20 uL 300 ul Approx 20 uL Approx 60 uL 1000 uL Approx 65 uL Approx 200 uL 1 200 uL Approx 140 uL Approx 220 uL 5mL Approx 0 3 mL Approx 1 mL 10 mL Approx 0 55 mL Approx 1 8 mL The counter provides information on the dispensing operations performed in the Pip mode You can switch the counter on and off using the rocker After you select the Pip mode an activated counter shows 0 The value can be changed in the Pip mode When ON is activated the piston moves to the basic position when a filled pipette tip is ejected A CAUTION Unintended dispensing when the Ejector reset option is activated gt When working with a removed ejector sleeve set the Ejector reset option to OFF to prevent unintentional dispensing from the pipette tip in case the ejector is accidentally pressed 5 17 5 History 5 17 6 Rocker setting 5 17 7 Sound level 5 17 8 Brightness If you activate the History option the History softkey will be activated instead of the Help softkey for most of the operating modes The last 10 dispensing settings of the active mode can be accessed using the History softkey The dispensing steps appear in chronological order Dispensing settings will only be stored if the History option is activated The change speed of the volume in the editing mode can be adjusted to suit your needs 8 le
40. elete a program mode 41 uone13d o Operation Eppendorf Xplorer plus Operating manual 5 16 Pipetting with fixed volume Fix dial setting Xplorer plus Only available for the Xplorer plus pipette Previously stored programs with fixed volumes can be executed in the Fixed volumes Fix mode Up to 10 different pipettings can be created see Editing Edit dial setting on p 39 3 fixed volumes are predefined upon delivery 10 of the nominal volume 50 96 of the nominal volume 100 96 of the nominal volume The aspiration speed sand dispensing speed vare displayed to the right of the fixed volume Requirement Pipette switched on see p 19 Fixed volumes Fix operating mode selected see p 42 Pipetting with fixed volume created see p 39 Pipette tip attached see p 24 Select the pipetting using the rocker Press the Select softkey Press the rocker up to aspirate liquid PON gt Press the rocker down to dispense the liquid 5 Press the rocker downward after the dispensing to execute the blow out Blow 5 17 Option Opt dial setting You can set the following options which apply to all operating modes in the Option mode Requirement Pipette switched on see p 19 Option operating mode selected see p 33 Select the option using the rocker Press the Select softkey to open the option Change the option using the rocker Press the End or Back softkey to exit the option c
41. er the maintenance and the gravimetric test 5 17 14 Date and Time Xplorer plus Only available for the Xplorer plus pipette You can set the date and time of the pipette Select and change the parameter as described above see p 19 48 Eppendorf Xplorer plus Operating manual 6 Troubleshooting 6 1 Reset The pipette will be reset to the initial state during the reset Your saved settings are not lost gt Press the Reset key using a pipette tip or another pointed object The personalization and software version will be shown in the display during the reset You will be prompted to press the rocker down in the display Make sure that any liquid in the pipette tip is dispensed correctly Then you need to confirm the Date and Time 6 2 Error search Symptom Possible cause Solution Display dark Pipette switched off Connect the rechargeable battery The rechargeable battery is not Switch on the Xplorer pipette connected Load the Xplorer pipette The rechargeable battery is Replace the battery discharged Battery defective Note If the rechargeable battery is almost completely discharged the display will only switch on after a short charging time Liquid is dripping from the tip and or the dispensed volume is incorrect The pipette tip is loose or does not fit correctly gt gt Attach the tip firmly Use epT P S Liquid
42. erating manual 5 11 Pipetting and mixing P M dial setting Aspirate a liquid and then dispense it again in the Pipetting Mix P M mode Afterwards an adjustable number of mixing cycles is automatically triggered The mixing cycles can be continued as often as required Aspiration Dispensing Release the rocker Blow out Auto mixing Requirement Pipette switched on see p 19 Pipetting Mix P M operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 Press the rocker up to aspirate liquid 2 Press the rocker down to dispense the liquid and start the selected mixing cycle The mixing cycles shown under Cycles in the main field of the display are reduced by 7 with each cycle 3 After the mixing process press the rocker down to trigger the blow out Blow If you keep the rocker pressed during the blow out the piston moves to the lowest position and remains there until the rocker is released O gt Press the rocker down to stop the aspiration Press the rocker up to stop the dispensing or current mixing cycle gt Hold the rocker pressed down during the mixing cycles to conduct an unlimited number of mixing cycles The current mixing cycle will still be completed after the rocker has been released 31 uone13d o Operation 32 Eppendorf Xplorer plus Operating manual 5 12 Manual pipetting Man dial setting In t
43. f danger DANGER Will lead to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage Symbols used Depiction Meaning gt You are requested to perform an action Perform these actions in the sequence described List Terms and key names from the software References useful information Eppendorf Xplorer plus Operating manual 1 4 Glossary Air cushion principle Biopur Blow out Cycle Free jet dispensing Increment ISO 8655 Multivolume Nominal volume PCR clean PhysioCare Concept amp A An air cushion is located between the piston cylinder system of the pipette and the liquid in the pipette tip Moving the piston creates a vacuum and may lead to liquid being aspirated into the pipette tip During the downward movement of the piston the air cushion is pressed downwards and the liquid in the pipette tip is dispensed Optimizing the piston stroke ensures that the stroke volume leads to the liquid volume shown in the display Pipettes in which the piston is brought into contact with the liquid to be dispensed are defined as positive displacement pipettes B Eppendorf Biopur amp is an Eppendorf AG purity level for consumables In addition to the requirements for standard goods e g precision accuracy wetting behavior
44. for the Speed parameter when using use free jet dispensing and if the geometry of the dispensing tube permits this action f you dispense liquid in the free jet the reverse stroke must also be dispensed in the free jet If a drop then forms on the pipette tip it is included in the first dispensing volume For wall dispensing trigger the reverse stroke with the pipette tips on the tube inner wall During dispensing do not switch between free jet dispensing and wall dispensing 28 Eppendorf Xplorer plus Operating manual 5 10 Pipetting Pip dial setting 5 10 1 Standard pipetting Aspirate a liquid and then dispense it again in the Pipetting Pip mode The blow out can be performed manually or automatically using the dispensing step A T Aspiration Dispensing Blow out Requirement Pipette switched on see p 19 Pipetting Pip operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 Press the rocker up to aspirate liquid 2 Press the rocker down to dispense the liquid If dispensing and blow out Blow are carried out in a single step hold the rocker pressed down during dispensing 3 If you keep the rocker pressed during the blow out Blow the piston moves to the lowest position and remains there until the rocker is released Hold the rocker pressed down to trigger dispensing and blow out Blow in
45. g and opening the 1 200 uL multi channel The method of loosening the 1 200 uL lower part matches that of loosening the 5 mL or 10 mL lower parts 1 The piston must be in its basic position before loosening the lower part Press the rocker down to move the piston into its basic position 2 With the ejector pressed down turn the lower part by approximately 30 and pull down to cancel the magnetic coupling This releases the lower part from the upper part 3 View the detached lower part from above For this slide the housing case down Note the exact position of the red marked parts The red marked stops on the housing case and the red marked teeth on the inner part are adjacent If the stops and teeth are on top of each other after assembly the housing case has been attached incorrectly Removing and installing 1 200 pL channels P Ga MM ch Eppendorf Xplorer plus Operating manual Housing case Piston actuator Inner part Piston rod in the guiding rail of the piston actuator al 5 Channel consisting of a piston a cylinder and a tip cone Fixing strip Base plate o 8 Unlocking tool for 1 200 uL lower part Only disassemble the channels when the upper part is detached from the lower part 1 Press the unloc
46. he Manual Pipetting Man mode use the Xplorer pipette like a manual dispenser The maximum aspiration volume can be limited if required Aspiration can be stopped or continued or the direction can be changed as often as required jt lt Aspiration Aspiration Dispensing Blow out O You can set a maximum aspiration volume using the Set max volume parameter The maximum aspiration volume will be displayed during the dispensing Max Requirement Pipette switched on see p 19 Manual Pipetting Man operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 To aspirate or dispense liquid press the rocker up or hold it pressed down The piston movement stops when the rocker is released The display shows the liquid volume in the pipette tip 2 Ifthe piston is in the basic position you can trigger the blow out Blow any time by pressing the rocker down If you keep the rocker pressed during the blow out Blow the piston moves to the lowest position and remains there until the rocker is released Eppendorf Xplorer plus Operating manual 5 13 Special operating modes The Xplorer plus pipette also features 5 additional special operating modes see p 21 If you have never worked with the Xplorer pipette we recommend becoming familiar with one of the simple operating mode first e g Pipetting or Dispensing If
47. he ON switch to turn on the pipette Power off Switch off the ON switch to turn off the pipette 5 2 Setting the Date and Time Xplorer plus Only available for the Xplorer plus pipettes Set the Date and Time parameters during the initial start up Requirement Battery connected see p 16 Battery charged see p 17 Pipette switched on see p 19 Select Date or Time using the rocker Press the Select softkey Change the value using the rocker Press the Next softkey to change the field Press the End softkey to end the entry Change the second parameter in the same way Press the End softkey to end the entry DUCQ Ov de co Jem O To change the date and time at a later time change the Date and Time parameter in the options see p 48 19 uone13d o Operation 20 Eppendorf Xplorer plus Operating manual 5 3 Principle of operation Every Xplorer pipette is equipped with various operating modes and a primary mode for configuring the Xplorer pipette The adjustable parameters and operational procedures differ in the operating modes During operation the piston in the pipette is moved according to how the rocker is actuated When you press the rocker up the piston in the pipette moves up Liquid is aspirated into the attached pipette tip When you press the rocker down the piston in the pipette moves down The liquid is dispensed into the pipette tip Pre
48. he Pipetting and Mixing mode P M as the program see p 39 Examples Required auxiliary aids Xplorer plus with a nominal volume of 1 000 uL Dispensing tube 1 5 mL 34 Eppendorf Xplorer plus Operating manual Example 1 total volume 500 pL dilution 1 10 e Diluent 450 uL Air bubble 300 uL 500 uL Sample or reagent 50 uL Example 2 total volume 900 pL dilution 1 10 Diluent 810 uL Air bubble 100 uL Sample or reagent 90 uL Example 3 total volume 1 000 pL dilution 1 10 Diluent 900 uL Air bubble 0 uL Sample or reagent 100 uL 35 uone13d o Operation Eppendorf Xplorer plus Operating manual 5 13 3 Sequential dispensing Spc dial setting Xplorer plus Only available for the Xplorer plus pipette Up to 10 different volumes can be dispensed in the Seg Dispensing mode The sum of the dispensing volumes may not exceed the pipette tip filling volume This mode is well suited for dilution series The Seg Pipetting mode is also well suited for dilution series To conduct a dilution series dispense two different liquids in the opposite direction This allows you to achieve different dilutions with the same volume per tube O If the sum of the dispensing volumes exceeds the volume of the pipette tip create a program and execute the mode several times in a row lj O A WV D JU Vw w UU www Requirement
49. he Xplorer plus pipette Two different liquids are aspirated in the Diluting mode These liquids are separated by an air bubble The Diluting mode is suitable for diluting samples and reagents using an appropriate dilution solution diluent First the diluent is aspirated then the air bubble and finally the sample or reagent O Because both liquids are aspirated in a single pipette tip the sample or reagent may be slightly contaminated by the dilution solution Diluent Aspirating the air Aspiration of the Dispensing bubble sample or reagent Requirement Pipette switched on see p 19 Diluting Spc operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 O For execution the parameter sequence is carried out in the reverse order of the editing process The next volume to be aspirated will be shown in the display with a border around it 1 Press the rocker up to aspirate the diluent 2 Press the rocker up to aspirate the air bubble 3 Press the rocker up to aspirate the sample or reagent 4 Press the rocker down to dispense all of the liquid 5 Press the rocker down to trigger the blow out Blow 6 If the piston is in the basic position press the Back softkey to exit the mode Set a high value for the Dispensing speed parameter to achieve a good mixture for the dispensing Ci To create a very high mixture you can enter the Diluting mode with t
50. he first or third measuring point You must carry out a gravimetric test to check the validity of the factors O You must gravimetrically check each Xplorer pipette whose factory setting has been changed by selection or by performing the above mentioned adjustments This is the only way to ensure that the selected adjustment meets requirements regarding errors Pswrd protection Xplorer plus Only available for the Xplorer plus pipette For the Xplorer plus the adjustment can be protected with a four digit numeric password The passwords for the Edit mode and Adjustment option may vary The password can be changed or deactivated after it has been entered Please contact Eppendorf AG Application Support or conduct a reset if you lose the password O The adjustment can no longer be changed if the password has been lost 1 Press the rocker upward to activate the password protection ON appears in the display Press the Select softkey Use the rocker to select the digit Press the Next softkey to switch to the next field Enter data in all of the fields Press the Save softkey to save the password Reenter the password after completing the saving procedure Press the Enter softkey noo bk o N Password protection is now active 5 17 10 Language Mark a language in the selection list with the rocker The selected language is only activated after the completion of the language selection o When selecting a
51. he same liquid again the blow out may remain in the tip If you use a different liquid discard the blow out and the pipette tip A Lk 1 Aspiration Dispensing Aspirate or empty Requirement Pipette switched on see p 19 Hev Pipetting Spc operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 1 Press the rocker up to aspirate the blow out and the liquid to be dispensed The aspiration of the dispensing volume and blow out will be displayed under the dispensing volume with the label Blow 4 2 Press the rocker down to dispense the liquid The piston returns to the basic position Liquid remains in the tip after the dispensing volume has been dispensed Press the rocker up to aspirate the same liquid Press the rocker down to end the pipetting and discard the blow out Blow O If necessary activate the Counter setting in the see p 42 options to count the executed dispensings using the pedometer uone13d o Operation 38 Eppendorf Xplorer plus Operating manual 5 13 5 Sequential pipetting Spc dial setting Xplorer plus Only available for the Xplorer plus pipette In the Seq Pipetting mode a maximum of 10 different pipetting volumes are dispensed in a fixed sequence This mode is well suited for dilution series The Seg Dispensing mode is also well suited for dilution series The random error precision and systematic error accuracy are better for pipett
52. ing a hazard to health potentially infectious agents organic solvents and reagents radioactive substances proteins presenting a hazard to health DNA 1 Please note the information in the document Decontamination certificate for product returns It is available in PDF format on our homepage www eppendorf com or on the enclosed CD 2 Enter the serial number of the Xplorer pipette in the decontamination certificate 3 Enclose the fully completed decontamination certificate for returned goods with the Xplorer pipette 11 2 Storage Damage to device due to inserted rechargeable battery during storage D gt Remove the rechargeable battery from the Xplorer pipette if you do not intended to use the NOTICE Xplorer pipette for an extended period Air temperature Relative humidity Atmospheric pressure In transport packaging 25 C 55 C 10 95 70 kPa 106 kPa Without transport b C 45 C 10 96 95 96 70 kPa 106 kPa packaging 68 Eppendorf Xplorer plus Operating manual 11 3 Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to
53. ing stand EE 18 A 51 D Diluting 25 alae ie ae i 34 Disassemble A ent 53 Disassembly 10 uL 100 uL and 300 uL multi channel lower part 57 1200 uL multi channel lower part 58 5 mL and 10 mL single channel lower part 56 Single channel lower part up to 1 000 pL 53 Disintect on c 51 Dispensing ido em 28 Brive Q 10 Borg rial ee 69 E Edic 39 Edit mode uiuo ei a 22 Error limits Multi channel sees 64 Single channel seen 63 F FOatures c 13 Fixed volume enter 42 FOOT fi E 12 H atelo E 11 L Lockingiellp i Hte e 54 LOCKING MING EM 54 M Main field cente ge une 11 Main illustration Multi chanrnel uite eoe 10 Gingole channel 9 Maintenance uscar 60 Manual plpetting rater ttr ciere retains 32 Multiple aspiration Aspirate seseeeess 33 O ON SWITCH EE 9 Operating mode icem ret eite its 21 Auto diepenelimg nenn 27 Dilitirig tds riesci ee 34 DISPENSING e M 28 Manual pipetting eee 32 Multiple aspiration Aspirate 33 P ipettind HS 29 Pipetting and Mitvimg ce eeeeeeseseeeeeeeeeteneeeeeeaeeeeaes 31 Reverse pipetting 2 ur444444HHHHnnnnnnnn nn rnnnnnnnnn nn 37 Sequential diepenslinmg eseese eener 36 Sequential pipetting esesseeeeses 38 Optio
54. ing than with dispensing The Seg Pipetting mode offers more flexibility with the volume selection than the Seg Dispensing mode Aspiration Dispensing Aspiration Dispensing Requirement Pipette switched on see p 19 Seq Pipetting Spc operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 Press the rocker up to aspirate liquid Press the rocker down to dispense the liquid Press the rocker downward after the dispensing to execute the blow out Blow Poe Wee If the piston is in the basic position press the Back softkey to exit the mode After each dispensing you can repeat the blow out Blow as many times as you would like by pressing the rocker down Observe the information on the blow out Blow see p 29 Eppendorf Xplorer plus Operating manual 5 14 Program Prg dial setting Xplorer plus Only available for the Xplorer plus pipette Previously stored programs can be executed in the Program Prg operating mode Programs consist of 1 4 combined operating modes in a defined sequence Up to 10 different programs can be created see Editing Edit dial setting on p 39 Requirement Pipette switched on see p 19 Program Prg operating mode selected see p 33 Program created see p 39 Pipette tip attached see p 24 1 Select the program using the rocker 2 Press the Select softkey The executi
55. ing than with standard pipetting These additional volumes may result in volume limits when using filter tips see p 43 During dispensing the blow out Blow does not belong to the dispensing volume Xplorer plus Use the Rev Pipetting operating mode for the Xplorer plus pipette see p 37 Keep the Release the Aspiration Dispensing Aspirate or rocker rocker empty pressed Aspirate down blow out Requirement Pipette switched on see p 19 Pipetting Pip operating mode selected see p 33 Parameters set see p 22 Pipette tip attached see p 24 Keep the rocker pressed down Immerse the pipette tip in the liquid Release the rocker to aspirate the blow out Press the rocker up to aspirate the dispensing volume Place the pipette tip on the wall of the target tube Press the rocker down to dispense the liquid Remove the pipette tip from the destination tube Residual liquid remains in the tip There are two courses of further action Sog Fb ob Aspirate the same liquid from the same reservoir tube Press the rocker up to aspirate the same liquid The residual liquid in the tip is used for further processing Dispense a different liquid 1 Press the rocker up to discard the residual liquid in the tip 2 Press the ejector to eject the pipette tip Use a new pipette tip for the next dispensing operation Eppendorf Xplorer plus Op
56. k procedure For service personnel only Not released for general use e Software update For service personnel only USB For service personnel only 5 17 13 Service interval Xplorer plus Only available for the Xplorer plus pipette If the Service interval option is activated the pipette will remind you about the required maintenance or repeating the gravimetric test You can set the reminder for a specific time or after a specific number of cycles The display shows the following options after Time interval or Cycles interval has been selected ast service Next service Set interval Heset For the Set interval parameter set the required interval If you would not like to use the Service interval option set the parameter value to 0 The interval will be reset when the reset function is triggered If the pipette should start counting at a specific initial value enter the value under Set interval and then select Reset You can view the last time a reset was triggered for the executed maintenance under Last service You can view when the next maintenance needs to be executed under Next service If the interval has elapsed up to 90 95 the pipette shows the next required maintenance in the display Monitoring of the interval time and the interval cycles function independently of each other If you activate the interval time and interval cycles you will receive a maintenance reminder for both settings A reset may be required aft
57. king tool as far as it will go into the base plate The base plate can now be detached by pulling gently at the side at the side with the unlocking tool inserted This loosens the base plate on the other side Remove the base plate Take the inner part out of the housing case Loosen the fixing strip from the inner part To do this push the fixing strip away from the inner part in the area near the three fixing strip catches on the inner part Have a close look at the channel arrangement in the inner part Four channels are accessible from each side The tip cone of the channels is eccentric to the cylinder The channels on the opposite side are therefore positioned mirror inverted Ensure that the channels are inserted so that they are aligned in the center again during subsequent assembly Completely press down the guiding rail for the piston rods Press the piston rod lightly out of the guiding rail and remove the channels 59 32u amp u3 uIelA Maintenance 60 Eppendorf Xplorer plus Operating manual 7 5 Maintenance 6 Before installation slide the piston into the cylinder Install the channels in reverse order in accordance with the previously mentioned instructions After installing the channels carefully push the guiding rail with the piston rods upwards to ensure that the magnetic coupling of the lower and upper part can be safely reestablished 7 Insert the reassembled lower part into the upper part The
58. l ur un LENZ CE eg CE TS LE cE LESS CE 7 LE Ge CS s K v r amp 7 ce C C CE C CE CC Cete CC ce CE ce C ce Ce CE Ce CE Te CE ee CE ee CS ce C eC EG Xo Te E GE GE C te ce C ce ce ce C Ce Ce CE Ire C ve CE ce CC ce C E C C C a ce o ce c ce d c c ce e C amp ce ce amp ce S T ce C ce ce GE EG Konformit tserkl rung c ce as EC Conformity Declaration TE j d 7 Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der f ce C aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten nderung des t C c ce Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit ce c The product named below fulfills the relevant fundamental requirements of ct pn ce C the EC directives and standards listed In the case of unauthorized modifications to the product or an unintended use this declaration becomes invalid ce ce Produktbezeichnung Product name 2011 65 EU pr pn E ck c co ck Me AEn ANY See Projektmanfgement Project Management Hi C Vorstand Board of Management 30 01 2012 ce C ce Hamburg Date ce i ce E C Ce Ce CE cC re ck c Eppendorf AG Barkhausenweg 1 22339 Hamburg Germany ct c Se x w C V c amp 7 C vt C6 ce CS oe VS CES a CG ett e E es ce
59. l be prompted to name the program You can enter a name with a maximum of six characters The program name cannot be changed later Copy the program to change the name You will be prompted to enter a new name Then delete the original program Select the characters using the rocker Press the Next softkey to switch to the next field Press the Save softkey to save the name Select the first operating mode All operating modes except Manual Pipetting are available Press the Edit softkey Change the parameters of the mode as usual see p 22 Oo N o o e oN Press the Save softkey A list with all of the previously saved program modes appears Press the Next softkey to add another mode A program can contain up to 4 modes Press the Save softkey to save the program The saved program now appears in the Program Prg operating mode see p 39 Changing the saved program Requirement To change the mode of a program the program needs to be saved first 1 Select a saved program from the list 2 Press the Select softkey Select the Copy option to create a program based on another program You must then enter a name for the copied program If you only want to change the name of a program select the Copy option 3 Select the Edit option 4 Press the Select softkey Select the Edit mode option to change a program mode Select the nsert mode option to add a mode to the program Select the Delete mode option to d
60. l pipettes from 100 uL upwards multi channel tool for cutting the O rings on the tip cones 1 Unlocking tool for opening the 1 200 uL multi channel lower part 1 Pipette wrench for opening the lower part on 5 mL and 10 mL single channel pipettes 20r3 Locking clip for lifting the spring loading on 8 or 12 channel lower parts with 10 uL 100 uL and 300 uL nominal volumes Eppendorf Xplorer plus Operating manual 2 4 2 5 2 6 Features Warranty Materials al NOTICE The Xplorer pipettes are motorized and electronically controlled piston stroke pipettes designed to aspirate and dispense liquid volumes according to the air displacement principle The Xplorer pipette family includes single channel and multi channel pipettes 8 or 12 channels Depending on the Xplorer pipette you can dispense volumes ranging from 0 5 uL to 10 mL The liquid is aspirated into a pipette tip which is attached to the Xplorer pipette The main control element of the Xplorer pipette is a rocker The color of the rocker differs depending on the volume range of the Xplorer pipette It has different functions during dispensing operations and in the dispensing parameter editing mode The rocker can either be pressed up or down The lower parts of the Xplorer pipettes are autoclavable For all Xplorer pipettes from 10 uL to 1 000 uL nominal volumes the tip cone responds with spring loading action when a pipette tip is attached
61. language the softkeys and the language selection list are always in English This makes it easier to return to the desired language if you select an unknown language by mistake 5 17 11 Personalization You can use the rocker to select letters and numbers to personalize the pipette The selected personalization will be displayed after the pipette has been activated from sleep mode or after the reset When the Xplorer pipette is delivered it is personalized as My Xplorer 47 uone13d o Operation Eppendorf Xplorer plus Operating manual 5 17 12 Service The options listed here are not needed during normal routine use A selection list offers the following service options Software version Display of the software that is valid for this Xplorer Parameter ver Display of the applicable data record for this Xplorer The data record is different for the various volumes Initial reset After a security query the pipette will be reset to factory settings and all entries will be reset to the default values This type of reset may be useful when transferring the Xplorer pipette to another workstation O The Initial reset function can also be executed if the Pswrd protection option is active Please contact Eppendorf AG Application Support if you would like to remove the password protection without losing saved settings and programs You will receive a Master key and additional information on how to proceed Chec
62. lues systematic error accuracy and random error precision and the test procedure for dispensing devices The total of the remaining stroke and the reverse stroke N The maximum dispensing volume of a pipette The nominal volume is also used as the name of a pipette The term nominal volume comes from the ISO 8655 standard P PCR clean is an Eppendorf AG purity level for consumables In addition to the requirements for standard goods e g precision accuracy wetting behavior and leak tightness products with the PCR clean purity level also meet the highest standards when it comes to sterility and being free of human DNA DNase RNase and PCR inhibitors Goods with the PCR clean purity level are inspected and certified by an external laboratory Lot specific certificates can be downloaded from our website www eppendorf com Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and make operation as simple as possible even with complex dispensing tasks PhysioCare products can be easily serviced and cleaned They are made of extremely light materials and require minimal operating force suononajsur Hurjyessdg Operating instructions Eppendorf Xplorer plus Operating manual Rechargeable lithium polymer battery Remaining stroke Reverse stroke Sterile Vapor pressure Viscosity Wall dispensing R This rechargeable battery is a special version of the rechargeable li
63. n AUS MO ae 45 Ee ele A4 9r TT 44 Date ana iMac 48 EJECTION OSCE MR 44 General help iau e ete 42 Ine M ET 44 Language vcnit tone eR Ote dioi ns 47 Personalization sissit irainaren 47 Pswrd protection icir iei esit eto tee toe 47 Hocker Seting ir 44 IS A eaaa gEe SNE 48 Service Intetval x adotta in teens 48 A DNE RES 44 Volume MI aia 43 P Parameter SOL iion n e Ee 22 Pipellinde 29 Pipetting and Mixing eee 31 Power off nella 19 Nee 19 Power supply assembly AA 16 Programmes 39 R rechargeable lithium polymer battery ore PED 17 Eppendorf Xplorer plus Operating manual o oeste ttai Oi ee 16 Replace channel 10 uL up to 300 uL multi channel lower part 57 1 200 uL multi channel lower part 59 KEE 49 Reset K6y sme neigen ad 9 Reverse pipetting eeeeeeeseeeeeseeeteseeeeeeneeeeeeeeenenees 30 37 S Selector wheel AAA 9 Sequential diepenslng nn 36 Sequential pipetting 2 r244nnennnnnnnnnnnnennnnnnnennnnnn nn 38 ser A 33 Speed table Multicchannel ecrire tenenda 66 Single channel s tnt 66 Spring mechanism essen 54 Standard poipetting 29 Status linia ld 11 A essen 51 SOMA ui 68 U USB Port B 9 Using Beie Ce aan 24 W EC E 13 71 xapu 5 Eppendorf Xplorer plus Operating manua
64. ng manual Xplorer Color code The adjustment for epTIPS long applies only to Nominal volume Xplorer rocker Color code Model Length Volume range epT I P S epT I P S epT I P S 10 uL medium gray light gray 20 uL Long 46 mm 0 5 10 uL 100 uL yellow orange 300 ul 55 mm 5 100 uL 300 uL orange not available 15 300 uL 1 000 uL blue dark green 1 250 uL Long 103 mm 50 1000 uL 1 200 uL green dark green 1 250 uL Long 103 mm 50 1 200 uL 5 mL purple not available 0 25 5mL 10mL turquoise turquoise 10 mL Long 243 mm 0 5 10 mL O You can select volumes lt 0 2 mL for the 5 mL Xplorer No technical data has been specified for the 0 2 mL 0 5 mL volume range The epT l P S specified here are also available as epDualfilter T 1 P S e Altitude The aspiration volume of a piston stroke pipette is reduced at increased altitudes which entail a fall in air pressure The average air pressure at the corresponding height is used to correct the stroke The altitude can be selected in 250 m 820 ft increments 5 000 m is the maximum altitude that can be selected The Xplorer pipette is set to an altitude of O m upon delivery This is the factory setting O The liquid type Ethanol 75 or Glycerol 50 epTIPS long and Altitude options can be combined For the following adjustments you must know the exact density of the solution to be dispensed The recording of the weighi
65. ng results requires the use of a fine balance with a high resolution Dispensing operations under 10 uL require a balance with a resolution of 0 001 mg An existing 1 3 point adjustment can be changed 1 point adjust After you enter the density selected volume and corresponding weighing results the Xplorer pipette will determine a correction factor The factor is only correct for the selected volume and the selected work technique However the factor is used for the entire volume range of the Xplorer pipette You must carry out a gravimetric test to check the validity of the factor 2 point adjust After you enter the density two different volumes and the corresponding weighing result the Xplorer pipette will determine a correction factor The factor is only correct for the selected volume range and the selected work technique However the factor is used for the entire volume range of the Xplorer pipette e below and above the two measuring points as well You must carry out a gravimetric test to check the validity of the factor Eppendorf Xplorer plus Operating manual 3 point adjust After you enter the density three different volumes and the corresponding weighing results the Xplorer pipette determines two correction factors The factors are correct from measuring point to measuring point in the selected volume regions and for the selected work technique The respective factor is however also used below and above t
66. ngs the random and systematic errors determined before delivery and the enclosed Eppendorf Certificate are once again valid The Xplorer pipette was calibrated before delivery and tested with double distilled water according to ISO 8655 Changing the adjustment of the Xplorer pipette is sometimes recommended for solutions with a density viscosity surface tension and or vapor pressure that vary greatly from that of water If the density of an aqueous solution changes by approximately 10 e g due to the salt concentration the volume changes by approximately 0 2 This statement does not apply if other relevant properties of the liquid also change If the pipette is used at extremely high altitudes it must be calibrated to the ambient air pressure At 1 000 m above sea level the volume error of a 100 uL pipette is about 0 396 Changing the adjustment can improve the dispensing accuracy systematic error when using tips that have a very different geometry than standard tips You can cancel a modified adjustment using simple steps Changes to the adjustment do not affect the precision random error of the dispensing The precision random error can be improved by replacing worn parts Furthermore handling has a strong influence on the precision random error Before the adjustment is changed the existing adjustment must be checked in the factory settings using distilled water The actual volume can be checked by weighing
67. of all dispensing parameters can be found in the Setting parameters chapter see p 22 11 uonduosap jonpo4d Product description Eppendorf Xplorer plus Operating manual 2 2 4 Footer Assignment of the softkeys with variable functions Some of the softkeys in the footer are Edit Open the edit mode Next Select next parameter End Exit the edit or help mode Help Show help History Display the last dispensing settings of the accessed mode see p 44 O The right softkey can be assigned to Help or History If the right Help softkey is activated the softkey can be used to display information on the selected operating mode If the History softkey is activated it can be used to call the most recently executed dispensings The assignment of the softkey can be defined in the options Opt The softkey is assigned to He p when the Xplorer is delivered 2 3 Delivery package Quantity Description 1 Eppendorf Xplorer 1 Rechargeable lithium polymer battery 1 Power supply with power plug adapters 1 English German operating manual Additional languages are available on the CD 1 CD with operating manual in several languages ordering information and additional documents 1 Short instructions in English Additional languages are available on the CD 1 Locking ring for deactivating the spring loading action for lt 1 000 uL single channel Eppendorf Xplorer pipettes 1 For multi channe
68. on of the program depends on the operating modes used d The blow out Blow must always be executed In the Pipetting mode 5 15 Editing Edit dial setting Xplorer plus Only available for the Xplorer plus pipette Programs and pipettings with fixed volumes can be created and edited in the Edit mode Stored programs can be executed in the Program Prg mode Stored programs with fixed volumes can be stored in the Fixed volumes Fix mode If necessary programs and pipettings with fixed volumes can be password protected using the Pswrd protection option Requirement Pipette switched on see p 19 Edit operating mode selected see p 33 1 Selected the required option using the rocker 2 Press the Select softkey The next steps are described in the following subchapters 39 uone13d o Operation Eppendorf Xplorer plus Operating manual 5 15 1 Pswrd protection You can protect the Edit mode using a four digit numerical password This prevents stored programs and pipettings from being changed The passwords for the Edit mode and Adjustment option may vary The password can be changed or deactivated after it has been entered If you lose your password please contact Eppendorf AG Application Support to request a temporary Master key password Have your pipette ready during your phone call with Application Support Or you can trigger an nitial reset all stored data is lost during an nitial reset
69. onde Gb hd ebbe ee vere 10 2 2 Display exse RR A RES RASA ERES EE A E ES 10 2 2 1 Header pn 11 2 2 2 STATUS IMS a CPC CEDE 11 2 2 3 EIER 11 2 2 4 A eR rx a ee Bude ne Fon me eh Me DR he 12 2 3 Delivery package oorr en ea ge 12 EE EE 13 25 Waranty essen O 13 26 Materials xxi mE AME a eb ele he ane ies RIA ae RE X REPE XE RES 13 Safety MP PITT 14 3 1 Inizto eBU i r 14 3 2 Warnings for intended use oocoococcnrrr eR I I nn 14 3 3 Information on product liability 15 Installation EE EE 16 4 1 Power supply assembly e He ad keane Nd NEEN Se e EES AEN 16 4 2 Connect rechargeable lithium polymer batter 16 4 3 Charging the rechargeable baten 17 nci 19 5 1 Switching the pipette on and oft 19 5 2 Setting the Date and Time 19 5 3 Principle Of Operation eeu were uen xo EE Y ee a dee qubd does oe Use aed re eS 20 5 4 Selecting the operating mode 21 5 5 Setting the parameter edit model 22 9 6 Jsmgpipelte tips decidas pa Mee Makes FREE AR EE EE E En RDR 24 5 7 Tipsforcorrect pipetting es rh Ir m nn 25 5 7 1 Preparation zzi a a ran dere Rd SEU x RENNES BE 25 5 7 2 pe unam 25 5 7 8 Dispensingiliguid us uu as REDE x RR E em a n edt E a a EROR EX 25 5 8 X Automatic dispensing Ads dial seng 27 5 9 Dispensing Dis dial setting eee RII II m t 28 5 10 Pipetting Pip dial eeng 29 5 10 1 Standard pipetting ooooocconcnrrn mmn 29 5 10 2 Reverse pipetting ce Re I nn 30 5 1
70. orer multi channel The technical data applies to both the 8 channel and 12 channel pipettes Model Test tip Testing Error limits Eppendorf AG Increment epT P S Color code Volume Error binis range Systematic error Random error 96 tpl t tpl 0 5 10 uL medium gray 1 uL 5 0 0 05 3 0 0 03 Increment 0 1 20 pL 5 uL 3 0 0 15 15 0 075 PEDE zul 10 uL 42 0 40 2 0 8 0 08 5 100 pL yellow 10 uL 2 0 0 2 2 0 0 2 Increment 2 200 uL 50 uL 1 0 0 5 0 8 0 4 SALE SC 100uL 08 0 8 0 25 0 25 15 300 uL orange 30 uL 2 5 0 75 1 0 0 3 Increment 15 300 pL 150 uL 1 0 1 5 0 5 0 75 pepe m 300uL 0 6 1 8 0 25 0 75 50 1 200 uL green 120 uL 16 0 7 2 0 9 1 08 Increment 50 1 250 uL 600 uL t2 16 2 0 4 24 HE zum 1200pL 12 14 2 0 3 3 6 Eppendorf Xplorer plus Operating manual Test conditions The errors specified apply only when the epT l P S pipette tips Test conditions and test evaluation in compliance with ISO 8655 Part 6 Test using a standardized fine balance with a moisture trap Check the volumes in the Pip mode keep the rocker pressed during the blow out Blow Set speed level 5 Number of determinations 10 Use of water in accordance with ISO 3696 Test at 20 C 25 C 0 5 C Dispensing against the tube wall Rechargeable battery Type Lithium polymer battery
71. quipment PPE ARNING gt Observe the national regulations for handling these substances gt Observe the material safety data sheets and the manufacturer s application notes Personal injury caused by the incorrect handling of the rechargeable battery N Do not disassemble or modify the rechargeable battery WARNING gt Never pierce crush or throw the rechargeable battery gt Only use the rechargeable battery in the Xplorer pipette Donottouch leaky rechargeable batteries Donotuse any damaged rechargeable batteries Dispose of damaged rechargeable batteries in accordance with legal regulations Danger to people from careless use DA Never point the opening of a Xplorer pipette fitted with pipette tip at yourself or anyone else AUTION Onlyinitiate dispensing if it is safe to do so For all dispensing tasks make sure that you are not endangering yourself or anyone else 14 Eppendorf Xplorer plus Operating manual A CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment gt Only use accessories and original spare parts recommended by Eppendorf
72. rer plus Operating manual The following information applies especially to high viscous solutions During the blow out Blow keep the rocker pressed down if the liquid is flowing out at a slow rate to ensure that the liquid can securely flow out If you keep the rocker pressed during the blow out Blow the piston in the pipette will remain at its lowest position gt Wipe any outer wetting against the wall of the tube while holding the rocker pressed down gt Leave the dispensing vessel with the rocker pressed down The rocker may only be released outside of the dispensing vessel The piston will only move to its basic position after the rocker has been released gt Press the rocker down again to repeat the blow out Blow as necessary O Stopping the piston movement Pressing the rocker in the other direction when the piston is still moving will stop the piston movement You can then move the piston either up or down by pressing the rocker in the corresponding direction Pressing the rocker up will abort a mixing process Operating modes with the rocker pressed down in the Man mode the ongoing piston movement is immediately stopped by releasing the rocker In the Ads mode an ongoing dispensing step is completed after the rocker is released The next dispensing step is only performed when the rocker is pressed accordingly Eppendorf Xplorer plus Operating manual 5 8 Automatic dispensing Ads dial setting In th
73. sembled power supply order number 4986 603 005 into a power outlet 2 Inthe Xplorer pipette check whether a rechargeable battery is inserted and connected 3 Insert the charging plug of the power supply into the connector socket of the Xplorer pipette The display briefly shows the connection with the power supply The charging process is indicated by a flashing rechargeable battery symbol in the display If the rechargeable battery is almost completely discharged the display will only switch on after a slight delay 4 Switch on the ON switch to observe the charging process The pipette can be charged in both switch settings 5 Charge the rechargeable battery for at least three hours The rechargeable battery will only achieve its full capacity after several discharge charge cycles 17 uonejeisu Installation Eppendorf Xplorer plus Operating manual single channel and multi channel Xplorer pipettes see Ordering information on the Xplorer f A 4880 series charger stand or charger carousel can also be used to store and charge vr CD 4880 charging stand 4880 charging carousel Eppendorf Xplorer plus Operating manual 5 Operation 5 1 Switching the pipette on and off O When not in use the switched on pipette automatically switches to the standby mode after a period of time The pipette automatically switches back on when it is moved Power on Switch on t
74. ss the Edit softkey to open the edit mode of the selected operating mode Here you can use the rocker to change the parameter that is outlined and marked red Special operating sequences with an electric pipette are possible with dispensing with the rocker For example you can cancel a piston movement in the Dis Pip and P M modes at any time by pressing the rocker in the other direction You can cancel the piston movement in the Ads and Man modes by releasing the depressed rocker After the piston has been stopped you can continue the aspiration or dispensing process by pressing the rocker in the corresponding direction If the piston is in the basic position you can repeat the blow out B ow at any time by pressing the rocker down The pipette software is available in several languages To change the language change the Language parameter in the options see p 47 Eppendorf Xplorer plus Operating manual 5 4 Selecting the operating mode You can set the desired operating mode using the selection dial The following operating modes are available Selection dial Mode Xplorer Xplorer plus Ads Auto dispensing Dispensing partial volumes in a defined e e interval Dis Dispensing Dispensing uniform partial volumes Pip Pipetting Aspiration and dispensing of liquid P M Pipetting and Mixing Pipetting with freely selected mixing volume Man Manual pipetting Aspiration and dispensing when th
75. ssed down A during mixing after O has been reached mixing is continued until the rocker is released If you press the rocker up during mixing the mixing process is stopped Gycies E Nex e End The mixing speed matches the selected speed for aspiration and i dispensing Set sample vol Diluting 4 a Volume of the sample or reagent Set sample vol The maximum potential diluent volume will be automatically set if the y 300 uL volume of the sample is changed Xplorer plus Next ep End 23 uone13d o Operation Eppendorf Xplorer plus Operating manual Set air volume Diluting T Volume of the air bubble Set air volume The maximum potential diluent volume will be automatically set if the y volume of the air bubble is changed Xplorer plus Air 200 uL Set diluent vol Volume of the dilution solution diluent Set diluent vol The maximum potential diluent volume will be automatically set if the y volume of the sample or the air bubble is changed egen Dil 400 uL Xplorer plus No dosage Seq Pipetting Number of executed dispensings A maximum of 10 steps are Number dosage available A The selected number of dispensings will be shown in red under Samples Samples 1i 5at the right of the display The active dispensing step will be activated by the number at the left Next ep End Xplorer plus 5 6 Using pipette tips The liquid to be dispensed is aspirated into pipette
76. sults are sometimes due to lack of maintenance 7 1 Cleaning Damage to device from unsuitable cleaning fluids or sharp or pointed objects ate Unsuitable cleaning fluids can damage the display surfaces and printing NOTICE Never use corrosive cleaning fluids strong solvents or abrasive polishes gt Check the compatibility with the materials used Do not use acetone to clean the Xplorer pipette gt Do not use sharp objects to clean the Xplorer pipette Remove any contamination on the outside of the Xplorer pipette as follows Weta cloth with a mild cleaning fluid and water and remove the contamination Toremove heavy contamination resulting from liquid penetration disassemble the lower part of the pipette see Xplorer pipette disassembly and assembly on p 53 and clean it with demineralized water Relubricate the piston sealing rings after contamination use of aggressive chemicals and or heavy stress Remove the old grease before relubricating 7 2 Sterilizing or disinfecting the pipette Damage to device from incorrect handling te gt Only autoclave the lower part of the Xplorer pipette NOTICE gt Do not use any additional disinfectants decontamination agents or sodium hypochlorite when autoclaving the lower part When autoclaving the lower part make sure that the temperature does not exceed 121 C gt Before using disinfecting agents or decontaminating agents test for material compati
77. th an ejector sleeve and ejector carrier The lower part must be removed in order to install a new ejector carrier Eppendorf Xplorer plus Operating manual 7 4 3 Multi channel Loosen and open the 10 pL 100 uL and 300 uL multi channel lower part 4 G and Slide the lever on the lower part to the left or right Pull the lower part down to cancel the magnetic coupling of the lower part to the upper part This separates the lower part from the upper part so that it can be removed Put down the lower part with the lever facing downwards O Slide the two latches right and left at the side down using a coin The lower part is still lying on the table with the lever facing downwards Remove the cover panel facing upwards with the integrated ejector rail Do not remove the ejector rail from the cover panel The spring for the ejector rail may accidentally come off and get lost in this step Removing and installing channels Channels must only be removed and installed if the multi channel lower part is separated from the upper part The channels in the lower parts consist of piston cylinder and spring The channels for 100 uL and 300 L are equipped with an O ring at the tip cone see Replacing O rings on p 52
78. the fine balance sufficiently sensitive balance resolution 0 001 mg e Were original epT P S pipette tips used for testing The pipette tip which must be used as the test tip for checking technical data is listed in the Technical data chapter in the operating manual No adjustment changes are allowed unless you can answer all the questions with yes In all other cases the problems associated with the questions answered with no must be eliminated If the problem is remedied by exchanging a complete lower part or other parts that have an effect on the volume proper assembly must be verified by carrying out a gravimetric test The systematic and random errors which must be met are listed in the technical data If you would like to conduct a 1 3 point adjustment for a liquid other than water on the Xplorer the items listed above should also be precisely observed when determining the balance results In particular please note that the density of the test liquid must be precisely determined at the test temperature The density is dependent on the temperature The use of an incorrect density will lead to an incorrect volume calculation The execution of the adjustment is described in the Adjustment document on the Xplorer CD Furthermore use the specifications in the Standard Operating Procedure for Pipettes document on the Xplorer CD as the foundation for determining the weighing results and errors
79. the liquid in a suitable tube For multi channel pipettes we recommend reagent reservoir Tip Tub as a liquid reservoir The liquid and the pipette should have approximately the same temperature Use epT l P S To minimize residual moisture in the tip after dispensing LoRetention for solutions containing surfactants Use ep Dualfilter T I P S to prevent aerosol contamination in the tip Use the pipette tips only once Check whether the set aspiration and dispensing speeds are suitable for the liquid The blow out Blow will be conducted at approximately the same speeds Wet the new pipette tip by aspirating and dispensing the liquid to be pipetted one to three times Vertically immerse the pipette tip into the liquid approx 4 mm during aspiration Press the rocker up to aspirate liquid Maintain the immersion depth to ensure no air is accidentally aspirated For large volumes keep the pipette tips in the liquid for approx 3 seconds after aspiration After aspiration slowly pull the pipette tip out of the liquid Wipe the pipette tip slowly against the tube wall to ensure that no outer wetting remains on the pipette tip When dispensing liquid place the pipette tip at a slight angle against the wall of the tube Press the rocker down to dispense the liquid After dispensing wait until no more liquid is dispensed Press the rocker down again to trigger the blow out Blow 25 uone13d o Operation 26 Eppendorf Xplo
80. the volume range from 0 5 uL to 10 mL in combination with matching pipette tips In vivo applications applications in or on the human body are not permitted The Xplorer pipette may only be operated by trained specialist staff All users must have read the operating manual carefully and familiarized themselves with the device s mode of operation 3 2 Warnings for intended use N Risk of explosion N gt Do not operate the device in areas where work is completed with explosive substances DANGER Do not use this device to process any explosive or highly reactive substances Do not use this device to process any substances which could create an explosive atmosphere Damage to health due to handling infectious liquids and pathogenic germs When handling infectious liquids and pathogenic germs observe the national regulations the pa biological security level of your laboratory and the material safety data sheets and the WARNING manufacturer s application notes Wear personal protective equipment Follow the instructions regarding hygiene cleaning and decontamination gt For complete instructions regarding the handling of germs or biological material of risk group Il or higher please refer to the Laboratory Biosafety Manual Source World Health Organization current edition of the Laboratory Biosafety Manual Damage to health due to toxic radioactive or aggressive chemicals Wear personal protective e
81. these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations may differ from country to country within the EU please contact your supplier if necessary E A b WARNING Risk of explosion and fire due to overheated accumulators and batteries Do not heat accumulators and batteries to over 80 C and do not throw them into fires Disposing of accumulators and batteries Ki Do not dispose of accumulators and batteries as household waste Dispose of accumulators and batteries according to the locally applicable legal regulations 69 jesods p pue 3Be40js 340dsue Index 70 Eppendorf Xplorer plus Operating manual Index A e E ue 61 E ele ii BIN LIRE ere tede 46 e ele 46 E eelere TEE 47 AU alcista 46 Ee ue EE 45 Ee 45 Factory Sets ioietan biridin aitai 45 Glyc rol 50 miae a ee Pe 45 Assemble OTING aiseee e a 53 Assembly 10 uL 100 uL and 300 uL multi channel lower part 58 1200 uL multi channel lower part 59 5 mL and 10 mL single channel lower part 56 Single channel lower part up to 1 000 pL 55 Auto dispensing sasssa torau nennen 27 C Charging carousel sse 18 Charg
82. thium ion battery Like rechargeable lithium ion batteries rechargeable lithium polymer batteries have a very high charging capacity and a long service life The special encapsulation of the rechargeable lithium polymer battery makes it lighter than rechargeable lithium ion batteries After dispensing steps have been completed any small quantities of residual liquid as safety reserves this liquid will be dispensed as the remaining stroke You can discard the liquid of the remaining stroke or reuse it After aspiration the piston is moved into a defined position Liquid is dispensed during this piston movement The reverse stroke is not a dispensing step S Sterile is an Eppendorf AG purity level for consumables In addition to the requirements for standard goods e g precision accuracy wetting behavior and leak tightness products with the Sterile purity level also meet the highest standards when it comes to sterility and being pyrogen free V This term refers to the pressure exerted by the vapor of a material solid or liquid in an enclosed container The vapor is in equilibrium with the solid or liquid phase of the material The vapor pressure increases when the temperature increases Each pure liquid has a vapor pressure of 1013 hPa mbar at boiling point Volume errors caused by high vapor pressure can be reduced by prewetting the tip Viscosity describes the viscidity of liquids and suspensions Dynamic or absolute viscosity is
83. tips We recommend using epT I P S epT I P S pipette tips which are available with various degrees of purity with and without filters and as special tips We recommend using special epT I P S LoRetention tips with liquids that have a lower surface tension than water e g due to a wetting agent in the liquid The pipette is only functional if a pipette tip has been attached You can either attach the pipette tip by hand or directly insert the end of the pipette into a tip held in the tip storage box If attaching a pipette tip by hand it must be handled in a way that prevents contamination and heating of the pipette tip The color of the rocker of the Xplorer pipette matches the color of the epT l P S tray Fit the suitable pipette tip s on the tip cone applying light pressure The pipette tip is securely fitted to the tip cone when it responds with spring loaded action exceptions no spring loading action with 5 mL and 10 mL single channel pipettes and with 1 200 uL multi channel pipettes You can deactivate the spring loading action of the tip cone on single channel pipettes Eppendorf Xplorer plus Operating manual 5 7 Tips for correct pipetting To achieve the highest precision and accuracy we recommend observing the following hints 5 7 4 Preparation Reagent reservoir Tip Tub gt 5 7 2 Aspirating liquid t 1 3 5 7 3 Dispensing liquid Always place
84. ure that you are authorized to carry out the adjustment The following message appears briefly for the selected volume Volume limit XXXX uL mL The Volume limit option was used to limit the volume to the displayed value 1 Make sure that you are authorized to change the Volume limit option 2 Move the selection dial to Opt 3 Select the Volume limit option Password incorrect Password for the Edit mode and the Adjustment option have been mixed up Password forgotten Enter the correct password Perform reset Contact Eppendorf AG Application Support and request a Master key Regularly check the precision and accuracy of the Xplorer pipette to prevent dispensing errors You can use the PICASO software version 2 3 18 or higher to determine the maximum permissible systematic and random errors If the pipette is used at a location with an extremely high altitude it must be adjusted to the ambient air pressure see Adjustment on p 45 R You can find an SOP Standard Operation Procedure for the test on our website www eppendorf com or on the Xplorer CD Eppendorf Xplorer plus Operating manual 7 Maintenance All single and multi channel lower parts are wear parts Clean them after contamination use of aggressive chemicals and or heavy stress If the lower parts are worn or damaged replace the respective parts Faulty dispensing re
85. ustment is selected in the options 1P ADJ 1 point adjust Single point adjustment by the user 2P ADJ 2 point adjust Two point adjustment by the user BF ADJ 3 point adjust Three point adjustment by the user Ey Glycerol 50 Adjustment to liquid type 50 96 glycerol Eth Ethanol 75 Adjustment to liquid type 75 ethanol EN Altitude Adjustment to an altitude other than 0 m above sea level Le epTIPS long Adjustment to an epT I P S long Volume 4 The selected volume for the acoustic feedback can be set in the options see p 42 If the volume has been set to 0 no loudspeaker symbol is displayed Battery Information on the charging capacity of the Li polymer battery Rechargeable battery fully charged qa Rechargeable battery partially discharged CH Rechargeable battery almost discharged Rechargeable battery must be charged see p 17 2 2 2 Status line The selected operating mode is displayed here during operation In edit mode the name of the parameter to be edited is displayed 2 2 3 Main field All parameters of the operating mode are displayed simultaneously during dispensing A black arrow WAindicates the direction of the next piston movement In edit mode the parameter to be edited is marked in red Some of the dispensing parameters in the main field include Dispensing volume Speed 8 speeds for aspiration and dispensing Counter Number of conducted dispensing optional setting in the Pip mode An explanation
86. vels are available 1 slow 8 very fast Level 5 is set upon delivery You can change the sound volume of the acoustic signals At 0 volume the sound is switched off and the symbol will not be displayed The display brightness can be adjusted to meet your needs Eppendorf Xplorer plus Operating manual 5 17 9 Adjustment d Changing the adjustment will change the dispensing volume and affect the accuracy of the Xplorer pipette Carry out a gravimetric test of the adjustment Every Xplorer pipette whose adjustment has been changed must be marked with a clearly visible label that indicates the type of change made This ensures that other users will be informed of the changes to the adjustment in addition to the information provided in the header of the display You can overwrite a selected adjustment by selecting another adjustment The applicable symbols for the new adjustment will be shown in the display header see p 10 For the Xplorer plus the adjustment can be protected with a password see Pswrd protection on p 47 CD Instructions on performing the adjustment can be found in the Adjustment document on the Xplorer CD The adjustment of the Xplorer pipette can be changed to suit your needs If you have disabled the factory settings the symbol and at least one additional symbol appear in the display header Before changing an adjustment make sure to read the chapters Display see p 1
87. with high vapor pressure and or different density gt Wet the tip several times and adjust the pipette for the liquid used Pipetted too quickly Set a lower speed Tip withdrawn from the liquid too early or too quickly Withdraw the tip from the liquid slowly and with a time delay of about 3 seconds for large volumes after aspiration Liquid dispensed without blow out in Pip mode Trigger blow out after dispensing has been completed during standard pipetting applications The piston is contaminated or damaged Clean the piston relubricate slightly and or replace The tip cone is damaged Replace the lower part or channel The O rings of the tip cones are damaged Replace the O rings only 100 pL 300 uL 1 200 uL multi channel Liquid flows unevenly into or out of the tip The tip is damaged The piston is contaminated The seal is contaminated The pipette is blocked Use a new tip Clean the lower part 5 mL and 10 mL sizes replace the protection filter Display shows the symbol The pipette has been adjusted for another liquid Check whether the adjustment selected in the options is correct and change it if necessary 49 Burjoousa qnoa Troubleshooting 50 Eppendorf Xplorer plus Operating manual Symptom Possible cause Solution No spring loading action of the tip cone when taking up pipette tips

Xplorer Plus User Manual

Contents

Download Pdf Manuals

Related Search

Related Contents