4100172 REV. C Benchmark Plus.DOC - Bio-Rad
Contents
1. Spee STE So TEM Sweden 40 05551 2700 Fu 46 1108 9582 27 GD Pi 001 777 9535 Fx odast United Kingdom Pr 0800 197 TJA Fe 442 2497 1300 SG2. Benchmark Plus Microplate Reader User Manual This Instrument for Laboratory Use Only 4100172 Rev C 4100172 Rev C Benchmark Plus System with Incubator Catalog Number 170 6930 Benchmark Plus Reader with Incubator Catalog Number 170 6931 Benchmark Plus System without Incubator Catalog Number 170 6935 Benchmark Plus Reader without Incubator Catalog Number 170 6936 Benchmark Plus User Manual Catalog Number 170 9504 Bio Rad Technical Services Department Open Monday Friday 8 00 a m to 4 00 p m Pacific Standard Time Phone 800 424 6723 option 2 option 3 510 741 6576 Fax 510 741 5802 E mail LSG TechServ US Bio Rad com U S LSG TechServ Intl 2 Bio Rad com International N otice No part of this publication may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopy recording or any information storage or retrieval system without permission in writing from Bio Rad Benchmark Plus is a trademark of Bio Rad Laboratories The Bio Rad logo is a registered trademark of Bio Rad Laboratories All other trademarks and registered trademarks are of their respective companies 4100172 Rev C 4100172 Rev C Warranty and Regulatory Notices Warranty Statement This warranty may vary outside of the continental United States Contact your local Bio Rad office for the exact terms of your warranty Bio Rad Laboratories warrants that the Benchmark Plus system will be free
3. 4 Packageinstrument disinfection certificate and accessories in the original box 4100172 Rev C 5 5 Product Information 170 6930 Benchmark Plus Microplate Spectrophotometer System 110 230 V with User Manual 170 6584 Serial Cable for PC 170 9520 MPM 5 1 PC Software with Manual 6 4100172 Rev C BIO RAD Bio Rad Life Seianca Web site www bioder Bio Rad Laboratories Main 2000 Alfred Nobel Driva Heroes 94547 Ph 510 7241 1000 Fr 510 741 5800 Group Also in Australia Ph 02 2914 2800 Fe 02 0074 2689 austria Fh 101 877 9 09 Fx 01 678 56 29 Belgium Ph 00 345 55 17 Fx 00 185 65 54 Brazil PA 55 21 507 5157 amada Ph 9005 712 377 Fr 05 Ghina Ph S 10 7201 71I3 8685 Fa 585 30 209 267 Danmak Ph 5 44 52 1000 Fr 45 4452 OO hakand Ph 254 A 04 ZZ Fr 355 GO 1100 Franca Ph Ji 475 BBS 27 47 47 TT Garman Phi 50 37S 64 077 Fe 089 378 84 123 Hang Kong Ph S5 2789 3300 Fr 50 2789 1247 Ch TARIST 1 Fa 01 224 6 90175 farmed Ph 03 8514124 Fr 03 351 4729 Ph AT 29005300 34 91 480 5217 dapan CHIA 70 Fx PoE Korea Oh 2 2 4 73 46 Fr AI AI Latin America 1804 5050 Ph 525 AM 2553 to H Fa 92 5 924 ST The Netheriands On 0710 0666 Ar A ST Mew Pn 61 94 150200 Fr fd 5007 Norveny Ph 47 23 38 4 30 di ZM 41 19 Hertie Ph 7 085 SA Mr 57 56 Singapore Ph 85 27 77
4. from defects in material and workmanship and will meet all performance specifications for the period of 1 year from the date of shipment This warranty covers all parts and labor In the event that the Instrument must be returned to the factory for repair under warranty the instrument must be packed for return in the original packaging Bio Rad shall not be liable for any incidental special or consequential loss damage or expense directly or indirectly arising from the use of the Benchmark Plus system Bio Rad makes no warranty whatsoever in regards to products or parts furnished by third parties such being subject to the warranty of their respective manufacturers Service under this warranty shall be requested by contacting your nearest Bio Rad office This warranty does not extend to any instruments or parts thereof that have been subject to misuse neglect or accident or that have been modified by anyone other than Bio Rad or that have been used in violation of Bio Rad instruction The foregoing obligations are in lieu of all other obligation and liabilities including negligence and all warranties of merchantability fitness for a particular purpose or otherwise expressed or implied in fact or by law and state Bio Rad s entire and exclusive remedy for any claims or damages in connection with the furnishing of goods or parts their design suitability for use installation or operation Bio Rad will in no event be liable for any spec
5. ial incidental or consequential damages whatsoever and Bio Rad s liability under no circumstances will exceed the contract price for the goods for which liability is claimed Regulatory Notices This instrument is designed to meet the Class A emissions and immunity levels of the EN61326 product family standard for the CE mark This rating requires that it only be used in a laboratory environment Important This Bio Rad instrument is designed and certified to meet EN 61010 requirements which internationally accepted electrical safety standards Certified products are safe to use when operated in accordance with the instruction manual This instrument should not be modified or altered in any way Alteration will Void the manufacture s warranty Void the regulatory certification e Createa potential safety hazard Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not 4100172 Rev C installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user wil
6. l be required to correct the interference at his her own expense 4100172 Rev C Table of Contents BENCHMARK PLUS MICROPLATE READER USER MANUAL eeeeoooooooooseooooooooooneooooooooooooeeeoooeooeooneeeeoeeoeee 1 GENERAL INFORMATION ssssseseocssssssceocsssesecoossssseceoossssseccoossssscososossssecoossssseceosssssseceossssssecossssssescossossssseossss 1 1 INTROD UC EON samas uel alale O nik ten 1 2 BENCHMARK PLUS SPECIFICATIONS a maaasikas E aa 2 EQUIPMENT SET UP AND INSTALLATION csssssssccssccssssensscessccssscensocnsccessscnsscenscoesssonsccenscossssenscseseoes 2 1 UNPACKING THE BENCHMARK PLUS MICROPLATE READER ananasa 2 2 EON OF EE NS J USING THE BENCHMARK PEUS nanja ja a A EA aaa a bei 3 1 EOADINGA PRA TE 3 2 SELECTING A WAVELENGTH IN THE BENCHMARK PLUS 3 3 READIN A EEA TE A 3 4 INCUBATOR CONTROLS una maa Sad INCUBATOR WOUND ama ae re a a kn ae 4 4 DISINFECTION DECONTAMINATION PROCEDURE eeeveeeeoooooooooonnnnoooooooooooooooooooooonnnonooooooeeeeeeeeee00 5 PRODUCT INFORMATION sina nasa 4100172 Rev 4100172 Rev C 1 General Information 1 1 Introduction The Benchmark Plus microplate reader provides all the flexibility required for absorbance assay The Benchmark Plus has
7. lems Plug the included power cord into the main power connector port Ensure that the instrument is connected to amain power outlet with a grounded conductor 2 2 Location of the Instrument The Benchmark Plus microplate reader is a precise optical measuring instrument The following conditions must be met to ensure maximum sensitivity Placetheinstrument on a flat surface that is free of vibration Do not placethe instrument in direct sunlight e Makesurethat the instrument is being operated in a dust free environment 2 4100172 RevC 3 Using the Benchmark Plus The Benchmark Plus is operated through the Protocol dialog boxes in Microplate M anager See the M icroplate M anager User Guidefor instructions on specifying the wavelengths selection and operating the Benchmark Plus through the Protocol dialogs Chapter 4 3 1 Loading a Plate To load a plate into the Benchmark Plus press the O pen C lose button on the front of the instrument or click on the Reader Door Open button in the Protocol dialog box in Microplate Manager The front door of the reader will open and the plate loader will extend outwards Place your microplate in the plate loader pressing lightly to set the plate into place Press the Open Close button again to close the reader door or click on the Reader Door Close button in the Protocol dialog in Microplate M anager gt The Benchmark Plus will accept any 6 well through 384 well plate with the dimensio
8. n condensing Range 4100172 Rev C TJ 2 Eguipment Set up and Installation 2 1 Unpacking the Benchmark Plus Microplate Reader Prior to opening the box check for any outer damage Document any damages if present Place the box upright with the TOP label facing up and open the box Remove the instrument from the box in this position only Warning Grasp the instrument firmly under the right and left sides before attempting to lift or carry the instrument out of the box Check the instrument for damaged or broken parts Document any damages in detail Each Benchmark Plus instrument includes the following accessories Verify that they are present 1Microplate Manager Software with manual Shipped in a separate box serial cable Shipped in a separate box 1power cord luser manual Verify that the serial number on the back of the instrument is the same as that on the outside of the box Located on the under side of the instrument is a docking bolt that needs to be unscrewed prior to use Counter Clockwise When the docking bolt can not be turned any further the scan arm will be free inside the instrument The Serial port is located on the back of the instrument There is one connector which allows the user to connect to one serial instrument Before switching the power on for the first time allow the Benchmark Plus to warm to room temperature for 6 hours after unpacking to prevent any condensation prob
9. ns 138 mm length x 98 mm width Plate height cannot exceed 15 mm 3 2 Selecting a Wavelength in the Benchmark Plus This section describes how to select the desired wavelength in the Benchmark Plus Since Benchmark Plus is amonochromator based system there shall be no need to physically change any filter through hardware means Instead all wavelength selections will be programmed with Microplate M anager M PM software Start up the MPM software Within the MPM select the desired scan method A window will appear that allows the user to select either single or dual wavelength scanning Within the window any wavelength can be selected within the 340 to 800 nm range See Section 4 2 3 Chapter 4 of the Microplate M anager User Guide for instructions 3 3 Reading a Plate Plate readings are controlled entirely by Microplate M anager See Chapter 4 of the Microplate Manager User Guide for instructions To stop a plate reading in progress press the Stop button in the Microplate M anager Protocol dialog box 4100172 Rev C 3 3 4 Incubator Controls The incubator in the Benchmark Plus is controlled from within Microplate M anager 3 4 1 Incubator Set Point The incubator set point is set from within Microplate anager The last set point selected in Microplate M anager is stored in memory inside the Benchmark Plus The Benchmark Plus will begin warming up to the last specified set point without opening Microplate M anager upon
10. restarting the instrument 4100172 Rev C 4 Disinfection Procedure All parts of this instrument that can come in contact with patient sera or positive sampl es must be handled as hazardous items Bio Rad Laboratories recommends using gloves when performing maintenance or working with the instrument gt Itis very important that the instrument undergo thorough disinfection before performing maintenance or before physical removal of the instrument from the laboratory The instrument must be disinfected before you send it back to Bio Rad Laboratories for service For safety reasons you must fill out a Disinfection Certificate and include it with the instrument Without the Disinfection Certificate Bio Rad Laboratories will not accept any returned instruments Bio Rad Laboratories recommends using a solution of 5 sodium hypochlorite solution in water Authorized persons wearing one way gloves and protective clothes must perform the disinfection procedure The location must possess adequate ventilation gt When disinfecting be sure to wear disposable gloves To disinfect the Benchmark Plus use the following procedure 1 RemovetheSERIAL cable power cord and any accessories 2 Clean the outside surface of the instrument carefully with a wad of cotton or a paper towel which has been soaked in the 5 sodium hypochlorite solution 3 Repeat the procedure for the disinfection on all accessories being returned with the instrument
11. the capability to perform standard colormetric ELISA and kinetic readings for general cell screening with 6 12 24 96 and 384 well microplate formats The Benchmark Plus uses a filter less design with a wavelength range of 340 800 nm Benchmark Plus is also provided with an optional incubation feature with temperature range up to 45 degrees Celsius The optical design provides verifiable results up to 4 00 D The filter less monochromator design allows the user to set the reading at any wavelength in the 340 800 nm region as well as do a spectral scan across any wavelength range within 340 800 nm to find the absorbance curve 1 2 Benchmark Plus Specifications Shaking Yes Optional Incubation Up to 45 C programmable in 0 1 C increments Power 100 240 V 60 50 Hz 2 0 Amps Dimensions 254 mm H x 457 mm W x 508 mm D Compatible Plates Any 6 through 384 well microplate with dimensions 138 mm length x 98 mm width Plate height cannot exceed 15 mm Linearity 840 800 nm Less than 2 or 0 01 OD from 0 3 0 O D Accuracy 340 800 nm Less than 1 or 0 01 OD from 0 3 0 O D Reproducibility 340 800 nm Less than 1 or 0 005 OD from 0 3 0 OD Software Compatibility A System Pentium 166 or higher 32 Mb RAM Windows 95 OS or higher Minimum Kinetic Read Interval 96 well plate 10 seconds single wavelength 30 seconds dual wavelength Environmental Operating 5 45 degrees C max relative humidity 80 no
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