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Welcome to the MicroChemi System

1. 3 Setup and Installation 10 Choose DNR Camera and click Have Disk Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a a disk that contains the driver you want to install click Have Disk Show compatible hardware Model This driver is not digitally signed Tell me why driver signing is important Insert the manufacturer s installation disk and then make sure that the correct drive is selected below 3 Setup and Installation 12 Browse to the following location My computer gt gt Local Disc C gt gt Program Files gt gt DNR gt gt GelCapture gt gt drivers gt gt FLI and click Open Date modified Type 04 10 2009 13 17 Setup Infc Libraries Ma Computer dy DNR Windows Le System32 New Volume D DVD RW Drive E Je images for MicroChemi drivers installation pro Setup Information inf 13 Choose fliusb driver and click Open Lookin Ji FLI 005c S Name i Date modified Di 04 10 2009 13 17 Recent Places Desktop Libraries fliusb 3 Setup and Installation 14 Click OK Insert the manufacturer s installation disk and then make sure that the correct drive is selected below Copy manufacturer s files from C Program Files DNR GelCapture drivers FLI 15 Choose DNR Camera and click Next Select the device driver you want to in
2. A McCrone 3 Setup and Installation 7 Click Next to continue installation Which components should be installed Select the components you want to install clear the components you do not want to install Click Next when you are ready to continue MooChemi CIA 8 Inthe Select Additional Tasks window choose if you want to create a GelCapture desktop icon Click Next to continue the installation Select Additional Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing GelCapture then click Next 3 Setup and Installation 9 Verify that the installation information is correct and click Install Ready to Install Setup is now ready to begin installing GelCapture on your computer Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Program Files DNR GelCapture Setup type Micro 10 The installation progress window will be displayed installing Please wait while Setup installs GelCapture on your computer Extracting files C Program Files DNR GelCapture MFC71U DLL s 3 Setup and Installation 11 Once installation is complete click Finish to exit Setup sc ccoo O deis Completing the GelCapture Setup Wizard Setup has finished installing GelCapture on your computer The application may be launched by selecting the in
3. YA Systems Welcome to the MicroChemi System Important Notice Copyright 2009 DNR Bio Imaging Systems Ltd All rights reserved MicroChemi is proprietary of DNR Bio Imaging Systems Ltd The information contained in this document is proprietary and is subject to all relevant copyright patent and other laws protecting intellectual property as well as any specific agreement protecting DNR Bio Imaging Systems Ltd DNR rights in the aforesaid information Neither this document nor the information contained herein may be published reproduced or disclosed to third parties in whole or in part without the express prior written permission of DNR Such information is supplied solely for the purpose of assisting explicitly and properly authorized users of the MicroChemi DNR reserves the right without prior notice or liability to make changes in equipment design or specifications The text and graphics are for the purpose of illustration and reference only The specifications on which they are based are subject to change without notice Information in this user guide is subject to change without notice Corporate and individual names and data used in examples herein are fictitious unless otherwise noted The software described in this user guide is furnished under license The software may be used or copied only in accordance with the terms of that agreement Other company and brand products are trademarks or registered trade
4. amp LPT b T Processors b Sound video and g p Ml System devices p Y Universal Serial Bus Scan for hardware changes Properties Uninstall 7 Inthe Update Driver Software window select Browse my computer for driver software How do you want to search for driver software Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device unless you ve disabled this feature in your device installation settings Browse my computer for driver software Locate and install driver software manually 3 Setup and Installation 8 Select Let me pick from a list of device drivers on my computer Icon selection D Update Driver Software MicroLine Browse for driver software on your computer Search for driver software in this location UENO i Include subfolders Y Let me pick trom a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device Select your device s type from the list below Common hardware types 3 DVD CD ROM drives c Floppy disk drives g Floppy drive controllers 05 Human Interface Devices ca IDE ATA ATAPI controllers amo IEEE 1284 4 compatible printer ga IEEE 1284 4 devices ij IEEE 1394 and SCSI printers IEEE 1394 Bus host controllers Infrared devices Keyboards ie
5. When the capturing process is complete the Average result of the Image Series will be displayed in GelCapture s main view b Click Acquire if you want to recapture the series or click the Return Pte button to go back to the Image Series main screen To capture an Image Series using Hardware Integration From the Capture Type dropdown list select Image Series Click the green CED Start button in GelCapture s main screen The Image Series settings will be displayed on the left side of the screen Use the Number of Images slider to select the required number of images 2 20 Mark the Hardware Integration checkbox The Start Time and End Time buttons will be displayed U Ae U N e Y Start Time End Time 4 Operating Instructions 5 1 Define the Start and End Times First adjust the exposure time sliders to define the start time Start Time and click the Start Time X4 button The Start Time refers to the exposure time of the first image 5 2 Next define the end time with the sliders and click the End Time fe Button The End Time refers to the exposure time of the last image 6 Adda time delay between images optional 6 1 Mark the Delay between images checkbox 6 2 The Set Delay window will be displayed where you can set the value for the time delay 7 Click the continue Ocot button In the DNR File Save dialog enter the name and location for the Image Series select any of the TIFF
6. Windows Defender Administrative Tools EB Color Management Default Programs dey Devices and Printers E Folder Options Y HomeGroup Keyboard XE Network and Sharing Center E Performance Information and Tools 3 Power Options a Region and Language E Speech Recognition User Accounts s Windows Firewall View by Small icons leg AutoPlay El Credential Manager E3 Desktop Gadgets E Display m Fonts 2 Indexing Options Location and Other Sensors Notification Area Icons a Personalization EW Programs and Features La RemoteApp and Desktop Connections 150 Symantec LiveUpdate El Windows CardSpace amp Windows Update 3 Setup and Installation 4 Select Device Manager from the left side of the window and click Continue in the next window balak e Ey i IT Control Panel All Control Panel Items System v Search Control Panel pl Control Panel Home F iS View basic information about your computer Device Ey Device Manager Windows edition Mana ger g Remote settings Windows 7 Professional System protection Copyright 2009 Microsoft Corporation All rights reserved a Advanced system settings System Rating System rating is not available Processor Intel R Core TM 2 Duo CPU 7400 2 80GHz 2 53 GHz Installed memory RAM 2 00 GB System type 32 bit Operating System Pen and Touch No Pen or Touch Input is available for this Display Computer name domain and w
7. brightness also increases and so does any electronic noise This results in a brighter image with less clarity Iris A mechanical diaphragm that adjusts the lens aperture with high levels of illumination the iris should be minimized with low levels of illumination the iris should be maximized Pixel A pixel or picture element is the basic unit that the camera uses to build an image Image size is also measured in pixels Pseudo colors Using pseudo colors to color an image artificially can reveal textures and qualities within the image that may not have been apparent with the original coloring Essentially the pseudo color module populates the image based on its grayscale value which maps to the full RGB color range Therefore red and yellow warm colors indicate oversaturated areas and blue cold color indicates under saturated areas in the image Green indicates midrange levels 8 Index B Binning 43 C Camera Calibration 33 from the Menu bar 35 Capturing an Image Series 46 an Image Series Hardware Integration 50 an Image Series Software Integration 47 Average 50 Summary 48 Automatic Exposure Time 41 Capture Type 45 Exposure Time 40 Images 40 CCD 6 33 67 E Exposure Automatic 41 Overexposure 62 Time 40 G GelCapture Features 9 Icons 10 Overview 8 I Imaging Tools 60 Image Statistics 60 Inverted Image 61 Overexposure 62 Pseudo Color 62 Region of Int
8. information is required a Quality choose this option when only general information is needed for example in verifying if there is isn t a given band Selecting Quality will provide the exposure time for producing an image that presents general information only 4 Operating Instructions b Quantity choose this option when detailed sample information is needed e g for analysis Selecting Quantity will provide the exposure time for producing an image that presents all the chemiluminescent information Auto exposure The calculated exposure time Is f Quality 24 seconds 4 minutes Cancel 5 Make your selection according to the requirements and click the Start Capture button to continue capturing an image or click Cancel to go back to GelCapture s main screen Binning Resolution vs Sensitivity Live science experiments include samples of both low and high intensity The Binning function in the MicroChemi system lets researchers choose various ratios of sensitivity and resolution for a given image which will improve the results The Binning process takes adjacent pixels and combines them into one giant pixel in order to increase the camera s sensitivity By creating a larger pixel you can create a higher ratio of Signal Noise for example when using Binning to create an area of 4 pixels 2X2 array This big new pixel which is created from 4 normal sized pixels increases the camera s sensit
9. verify its compatibility with Windows xP Tellme why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation N 3 Setup and Installation 4 Once the Wizard completes the drivers installation click Finish Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for DNA Camera Click Finish to close the wizard Cancel 5 Double click the desktop GelCapture icon to open the program GelCapture will now recognize the camera and its drivers 3 Setup and Installation Installing the Camera Drivers Windows 7 Professional The camera drivers are installed automatically If the camera drivers are not installed Note sd automatically please follow the following installation instructions Before installing the camera drivers you must first install GelCapture software for Windows 7 Professional on your computer as described on page 17 To Install the Camera Drivers Windows 7 Professional 1 Goto Windows Control Panel by clicking Start gt gt Control Panel Getting Started Connect to a Projector 7 Documents Calcul
10. 62 iage TOO ana a a a aslo aa a a i 63 image SUOC SECS HA A ET E T T EN 63 Inverted IMAJ E nran n o nn eo E TN 64 PSU COMO A A NS 65 OVCT CKD OS UIC sit TOTO 65 REGION OF INTCTESE ROI si ali 66 5 Maintenant ensena acecauss eaten deena E tor eared a aucauns DTE EE 67 NicroCnemiLAnnual Manten anciana AE Aa aaa aaaea aaah 67 6 MFOUDICSMOOCINNE ia ASA AAA 68 7 CIO aii iia 70 8 O eae cscs E san aan csane en E seas veus vice uscs sinc vans uses woes vena viees E E vans sana E E E san suauaverene 71 1 Introduction The MicroChemi system is a compact and affordable gel documentation system that supports chemiluminescent and bioluminescent applications The system combines the MicroChemi Unit and GelCapture software intuitive and easy to use image detection software to create a compact and fully integrated imaging solution The MicroChemi system is packaged with a CD containing both a Quick Reference Guide and this User Manual To better utilize your system read Chapter 3 on Installing MicroChemi and GelCapture before proceeding with the rest of the manual Once your system is installed and operational you can then familiarize yourself with the system s different features and procedures Everything you need to know about your MicroChemi system is included in this manual The highlighted chapter below contains information you may need to refer to in your daily use of the MicroChemi system and is therefore also provided as a Quick Reference Guid
11. File Save Options and click Save 8 The capturing process will start and the images will be displayed in GelCapture s main view You can abort the process by clicking the Abort CE button and only the images that were captured up to that point will be displayed in the main view 9 After aborting the process you can click the Acquire asi button to start the process from the beginning using the values you already set This will overwrite the images that were already acquired in the aborted process unless you change the Image Series name 10 When the capturing process is complete click the Return Pte button to go back to the Image Series main screen You can define a long or short Start Time and a long or short End Time For example you can set the Start Time to 50 seconds and the End Time to 2 seconds to produce a series of images of decreasing exposures OR set the Note Start Time to 2 seconds and the End Time to 50 seconds to produce a series of images of increasing exposure By default the exposure times of all the other images is automatically defined by GelCapture Use the Set Time window only if you wish to define a different exposure time for a specific image as explained on page 50 4 Operating Instructions Saving Features Most software programs have Save and Save As options for saving a file In addition to the standard options GelCapture has the Save Image with Reference feature which lets you create and save
12. PL 3 Setup and Installation Hardware Setup To set up your MicroChemi unit plug in the MicroChemi power supply cable to the unit and the wall and connect the USB 2 0 cable to the PC as illustrated below Pre connected Camera USB cabl MicroChemi power supply socket MicroChemi MicroChemi MicroChemi ventilation fuse niche ON OFF switch Software Installation After the MicroChemi unit is set up you can proceed to install the following software from the CDs provided inside the MicroChemi package e GelCapture Windows XP Pro page 13 Windows 7 Professional page 17 e Camera drivers Windows XP Pro page 23 Windows 7 Professional page 26 e Analysis Software an explanation on the installation process can be found in the CD Note Turn the MicroChemi unit OFF before you install GelCapture 3 Setup and Installation Installing GelCapture from CD Windows XP Pro The installation process requires constant feedback and input it is therefore advised to closely oversee the installation process Note Before starting installation verify that you are logged in as Local Administrator 1 Insert the installation CD into the disk drive The installation wizard starts automatically In the Welcome window click Next to continue ee Setup GelCapture shih om Welcome to the GelCapture Setup Wizard This will install GelCapture 6 5 on your computer lt is recommended that you close all other applications b
13. Peak 65535 Min 0 Max 65535 Medar 2093 Min HA ux OE Contrast Stretching Set step size 100 757 550 Pixel 2005 4 Operating Instructions To View an Image Using the Overexposure Tool 1 Click the Overexposure icon 2 Click the Overexposure icon again to revert to the original view of the image Region of Interest ROI The ROI feature lets you define a specific area of the sample in order to perform various actions on it either in Preview mode Start mode or on a saved image In Preview mode you can view the Histogram of the selected area In Start mode or with a saved image you can crop save copy and view the Histogram The Revert option is only available with a saved image To Define a Region of Interest r71 LJ 1 In Preview Start mode or in a saved image click the ROI icon A green frame will appear on screen as seen in the image below 2 Adjust the image size or position You can either stretch the pivot points on the frame or mouse left click and drag down to open a new frame of a region of interest 3 Point the mouse anywhere within the green frame and right click to display the menu options ROI Options available in ROI Options available in Preview mode Start mode Pirstogram 5 Maintenance MicroChemi Annual Maintenance To keep the MicroChemi unit in good working order observe the following guidelines 1 Camera Calibrate the camera once a year as described i
14. a eo lt P Return E swe Y E S econds gt s zl Standard Resolution z Camera Ready T y Count 216064 Edge 301 6 Peak 512 Median 55391 Restore Original Contrast Stretching Set step size 100 BERL C AR OT TTT AM OTTO 577 17 Pixel 49 4 Operating Instructions Quantity Gray Level Count Edge Peak Min Max Median Min Max sliders Restore Original Contrast Stretching Set step size Gray level amount Gray level type 0 65 535 The number of gray levels in the image Refers to image sharpness The grayscale value with the highest number of pixels Indicates the lowest reading in the statistical curve Indicates the highest reading in the statistical curve A maximum of 65 535 indicates the highest rating The median grayscale is the value in the exact middle between the maximum grayscale and the minimum grayscale of the image Manually change the image stretch level by clicking the right or left green arrows or by moving the slider Moving the Min Max sliders will result in different image Min Max gray levels Restores any stretching actions to the original image values Auto increase the stretching of the image This option helps reveal low concentrations of biological samples in the gel Control the step size of each click on a given Min Max slider arrow by setting the step size to a value between 1 and 1000 Inverted Image Inverte
15. able the system creating calibration files click No Yes NO 3 Setup and Installation 3 When you choose option 2 1 click Yes to load the camera calibration files from the CD A dialog box will be displayed asking you browse to the Camera Calibration CD Click OK and GelCapture will begin importing the camera calibration files Browse for Folder Please select the folder to import the settings From My Documents My Computer E My Network Places D Calibration procedure screen shots 4 Sy MLOD12109 4 5 PLO 50308 new MOP 4 After the camera calibration files have been imported successfully a message box will inform you that calibration files have been created successfully Calibration files have been created successfully 5 When you choose option 2 2 in section 2 click No to enable the system to create the calibration files A CCD Calibration message will be displayed where you will need to select the calibration type you want to execute either Short 135 minutes or Long 8 hours and click Acquire CCD Calibration E CCD Calibration E a gt z y A r PF Dp ee NS IAS y4 E AN E N R Chapt eet oe een E a La W laln td 1 9 y o Bigg EAS Siete LY Line Til MLL a rari AIU LS AAA A e ea E A 3 Setup and Installation 6 Every time you mark the long calibration 8 hours box and if you have not previously performed short calibration the Short calibration 135 minutes box will a
16. amera is ready 2 Place the sample in the center of the Drawer Tray Drawer Tray 3 Close the drawer and start the GelCapture application on your PC 4 Operating Instructions Previewing an Image Preview mode lets you verify that the sample is located correctly before capturing the image Before you click the Preview button make sure the sample is positioned correctly on the Drawer Tray To Preview an Image 1 Turn the White Light WL button on Dimmer WL ON OFF switch 2 Click the Preview Q reven button In GelCapture s main screen A message will be displayed reminding you to turn the illumination ON Preview GelCapture File Edit View Help z RHAG Mai lt P sup 3 Adjust the illumination intensity by turning the Dimmer dial 4 When you are satisfied with the location of the sample click the Stop lt P button to return to GelCapture s main screen 4 Operating Instructions 5 The following message will be displayed in GelCapture s main screen asking you to turn the illumination OFF Please turn off the illumination Before you click the start button to capture the image you must first set the Exposure Time Note o Binning and Capture Type 4 Operating Instructions Capturing Images When capturing a new image you may often want to adjust several parameters such as Exposure Time Capture Type and Binning Resolution and Sensitivi
17. an image of a sample that was stained with both chemiluminescent and visible dyes This process creates a new image which is actually a combination of two separate images Read the following section to learn more about the saving options To Save an Image After setting your preferences Exposure Capture Type Binning continue to capture the image and save it in one of the following manners 1 After the image is captured and you are happy with the results click the Save k icon or the i Save E _ button The DNR File Save dialog window appears DNR File Save Save in E MicroChemi P c Ed 6 My Recent Documents E Desktop D My Documents y My Computer ES L My Network File name Image v Places Save as type TIFF Files tif Cancel TIFF File Save Options Super resolution Spot Filter Add Parameters To Image Crop Image to view size 2 Browse to where you want the image to be saved enter a name for the image and select the required Saving Options see explanation below 3 Click Save The file is saved to the selected directory 4 Operating Instructions Saving Options In addition to saving the image according to your settings GelCapture lets you define other saving parameters which are selectable from the DNR File Save window dd etwork File name Image v laces Save as type TIFF Files tif X Cancel TIFF File Save Option
18. an image e Turn the MicroChemi Unit ON e Turn the MicroChemi Camera ON e Insert a sample e Open GelCapture e Preview the image e Capture an image e Save the image Note The system is equipped with fixed iris and focus Turning the MicroChemi Unit On Turn the unit ON by pressing the ON OFF switch which is located at the back of unit MicroChemi ON OFF switch Note The system can be left ON and running continuously for 1 month 4 Operating Instructions Turning the MicroChemi Camera On The Camera ON OFF switch is located at the front of the unit Camera ON OFF switch Every time you turn the MicroChemi unit ON you must allow the camera about 5 minutes cooling down time before it stabilizes at operating temperature Operating temperature ranges between 50 C and 60 C When the temperature is higher than this range the temperature indicator is red When the camera reaches operating temperature the indicator turns green Live Off GelCapture File Edit View Help Live Off GelCapture File Edit View Technician Help PHA Mai ICC DN ai D Preview 1 Minute v Single Image r Standard Resolution y CD Preview 1 Minute v Single Image v Standard Resolution v sr e Red temperature indicator the camera is not ready Inserting a Sample 1 Pullto open the Drawer EA Green temperature indicator the c
19. andard Resolution the image will be detected in very high resolution and normal camera sensitivity Other options such as High Sensitivity Super Binning etc will increase the camera sensitivity but decrease the resolution as shown in the following table ame sete Reto High Resolution 8 MP Standard Resolution 2 MP High Sensitivity 0 5 MP Super Sensitivity 222222 P 4 Operating Instructions Following are examples of an image captured with High Resolution and an image captured with Max Binning all other parameters were identical High Resolution Max Binning 2 e e A we a a m ae me Capture Type Single Image and Image Series MicroChemi lets you capture either a Single Image or an Image Series When capturing an Image Series you can use either the Software Integration or Hardware Integration option depending on your project s requirements See the explanation on Integration types on page 49 Capturing a Single Image 1 From the Capture Type dropdown list select Single Image Single Image v Single Imaqe Image Series 2 Click the green Start CES button in GelCapture s main screen The image capturing process will start and display an image in GelCapture s main viewing area 3 After the image is captured by MicroChemi click the Return button to return to GelCapture s main screen You can now set new parameters and click start to capture a new image WE Acquire im F
20. ard image If the standard can hardly be seen in the chemiluminescent image adjust the Threshold slider and click Copy or Copy Inverted again Threshold helps you reach the ultimate dynamic range ratio between the chemiluminescent and white light images Reference Image Save a kuss o aap a CEED www SSI APARATO pee nd BORSE qa ASA a 195555586868 AS Ea a OD RRES am aes amen CAGAS quay bees a votes soebseaseasse ies enn n ACE A ARCA a SS SSS ey r Bio Imagina Systems 11MM H t A y ad o o Copy the Reference and press Done when finished f 4 White Light image Done Cancel Revert Copy AANDEL BELULIN Target image adjustment displayed in the Save Image with Reference viewer Copy Inverted option 4 Operating Instructions Reference Image Save gt PESTER O somes Camm Gp GD m A dd j anaana CNE SEREARSRARIIGRE CARTIER AO ESSEER SSSA O OOS ammm AA y arkaa ainei wea CO a pis Beene Done di kk le ee ee n Sm PA EA A RI BDO Cancel Revert SIA ds PO VARAS SSR PJ Copy Copy Inverted Copy option AN VT LUTE AECE Pant Gee 10m Copy the Reference and press Done when finished Target image adjustment AAA The chemiluminescent image on the right side will display the sample standard which is stained with a visi
21. ator Pictures Sticky Notes Music Snipping Tool Computer Paint Control Panel XPS Viewer eee Ree gs settings a Windows Fax and Scan Default Programs Remote Desktop Connection Help and Support GelCapture gt All Programs 3 Setup and Installation 2 Select Small icons from the Control Panel View by list Me ES gt Control Panel fers AM N Adjust your computer s settings System and Security Review your computer s status Back up your computer Find and fix problems Network and Internet View network status and tasks Choose homegroup and sharing options Hardware and Sound View devices and printers Add a device Programs Uninstall a program 3 Double click the System icon User Accounts and Famil BY Add or remove user accounts E Set up parental controls for a Small icons Appearance and Personalization Change the theme Change desktop background Adjust screen resolution Clock Language and Region Change keyboards or other input methods Ease of Access Let Windows suggest settings Optimize visual display mr Z E Control Panel All Control Panel Items p Search Contro Panel p Adjust your computer s settings Pe Action Center yes Backup and Restore E Device Manager a Ease of Access Center Aq Getting Started E Internet Options P Mouse S Parental Controls IE Phone and Modem p Recovery Sound Sync Center Troubleshooting il
22. ble dye 10 When you have finished copying all the required standards click Done If you want to start the standard copy process from the beginning click Revert If you wish to cancel the Save Image with Reference process click Cancel ME Reference Image Save aaa cm meet e AR PELIS PUMAS PS _ MERRE o CID ae GED omw A mks ps GD GD a TEER annA ee AAA EUR G eC eee eee organ TS SA CENT ci A ERRE qa A RA gt aces i shia PRESI Done Done n ERAEN PAR O REC a SaaS seme IA Cancel ae 3 Revert AAA eS JH Copy Copy Inverted 800 A EERI E A 4111 REEL yA Systems a o s lt 2 mo l AA ts Copy the Reference and press Done when finished tom Target image adjustment N 4 Operating Instructions 11 After clicking Done a pop up message will appear and ask if you are happy with the combined image or if you would like to add more standards If you click Yes the combined image will be displayed in the main viewing area and you can proceed If you will click No the last screen will be displayed and you will need to select the image standards again CiWocumnents and Settings Wer WDerdcopMicschemilimage REF 1 GetCagture AWAD wa AAS EO GelCapture y Dre E rconten Parameter Exe nei Camera Exonse 000400 Segle image l Erreg Standard Resubten 12 When you accept the image by clickin
23. d image inverts the color scale i e light areas appear dark and dark areas appear light much like a negative This feature is particularly useful when you need to decipher close bands To Invert an Image 1 Click the Invert Image icon Click the icon again to revert to the original view of the image 4 Operating Instructions Pseudo color Pseudo color lets you view an image in one of two ways 1 One color such as orange green or blue similar to the actual color of the sample For example samples dyed in Coomassie Brilliant Blue are colored blue with pseudo color 2 Color spectrum indicating the hot and cold areas of the image In this mode the colors are based on the image s grayscale where red refers to the maximum and blue refers to the minimum grayscale levels To View an Image in Pseudo color 1 Click the Pseudo color E icon 2 Click the Pseudo color icon again to revert to the original view of the image Overexposure Overexposure occurs when the camera receives too much light which usually results in white or bright areas This phenomenon leads to inaccurate results when analyzing the image information In order to avoid this distortion GelCapture is equipped with the Overexposure tool which displays overexposed areas in red Acquire image GelCapture File Edt View Help gt bel A at abs lt P Return Y 5 Save i gi Quantity Grey Level Count 477204 Edge 311 6
24. e drawer is inserted properly 7 Glossary CCD A charge coupled device CCD is a light sensitive integrated circuit that stores and displays the data for an image in such a way that each pixel in the image is converted into an electrical charge with intensity relative to a gray level in the grayscale The MiniLumi system has a black and white sensor with 65 536 gray levels so there will be a separate value for each gray level that can be stored and recovered CCD is one of two main types of image sensors used in digital cameras to create high quality low noise images Dynamic Range Dynamic range is the range that a sensor is capable of differentiating If the sensor can differentiate between two points in an image then each point is translated into a pixel of the image if the sensor is unable to differentiate then the two points will be pixels of noise Dynamic range is also referred to as Signal to Noise Ratio SNR Exposure Time The length of time light passes through the lens to form an image Fluorescence A process by which a fluorescent molecule is excited at one wavelength and triggers the emission of a photon with longer less energetic wavelength making it possible to detect image and measure the amount of fluorescence in samples as small as individual cells and with multiple fluorescent colors Gain A camera feature that increases or decreases the strength of an electrical signal When the gain is increased
25. e for your convenience Chapter Topics Covered 1 Introduction An introduction to the manual and the topics covered in it 2 Overview A brief look at the system s key features 3 Installing MicroChemi and A step by step guide for installing your system GelCapture 4 Operating Instructions Operating instructions from basic to advanced 5 Maintenance Guidelines for maintaining good working order 6 Troubleshooting A list of the most common issues and how to resolve each one 7 Glossary A list of terms used in this guide and their meaning 8 Index 2 Overview The compactly designed and easy to operate MicroChemi unit houses a high resolution camera which is fully controlled by the GelCapture software a super bright lens and an advanced dark chamber all producing the perfect solution for high quality imaging results System Overview The gel sample is placed on the MicroChemi Drawer Tray and the GelCapture image detection software detects and saves the specimen images which can then be exported to other software for analysis Image detection settings can also be saved and later recalled for further use Detect and capture the specimen s image Placea sample on the Drawer Tray ive Off GelCapte ee SA AAA Pid A SMa QQ Sga Grey Count 0 Edge 00 Peak 0 Min 0 Max 0 Median 0 va E _Restore Original Contrast Suetching Set step size fico 2 Overview Featu
26. ed Then by clicking the ECL standard icon a white light image is captured without moving or touching the sample The two images are then combined and displayed in the main viewer window 4 Operating Instructions To Save an Image with Reference 1 Place the sample on the MicroChemi Sample Tray 2 Capture a chemiluminescent image A in ar a r de Captur Fie Edit View Help 2 bl Bar Aa Sun A Grey Level Count 412672 Edge 4257 Peak 512 Mei 0 Max 750 Madan E3344 GE wa GE E Restore Onignal Connan Stretching Set step size fico teal e Hot 1593 620 Pixel 50 AATEC BERR A 3 Click the Save Image with Reference icon on the GelCapture tool bar 4 A pop up message will be displayed asking you to verify the illumination is turned on prior to taking the reference image Please verify the illumination is turned on before taking the reference image 5 The save dialog box will be displayed 4 Operating Instructions 6 Select the folder you want to save the image to and click Save Organize v New folder S Name Date modified Y Favorites BZ Desktop No items match your search lg Downloads Recent Places 3 Libraries Documents a Music t Pictures F Videos j Computer a ni g v r File name bs Save as type TIFF Files tif v a Hide Folders Cancel 7 The sample which is illuminated with WL is displayed in the Save Image with Refer
27. efore continuing Click Next to continue or Cancel to exit Setup 3 Setup and Installation 2 Click Next to install to the default location or click Browse to select a different location Select Destination Location Where should GelCapture be installed A Setup will install GelCapture into the following folder To continue click Next If you would like to select a different folder click Browse At least 43 9 MB of free disk space is required 3 Which components should be installed Select the components you want to install clear the components you do not want to install Click Next when you are ready to continue MicroBIS MiniBIS MiniBIS Pro MiniLumi LumiBiS LumiBIS with HeptaLED ChemiBIS 0 5M F ChemiBlS 0 5M F ChemiBlS 0 5M with HeptaLED MChemiBlS MF ChemiBlS MF ChemiBlS with HeptaLED FChemiBIS M FChemiBIS M with HeptaLED icrolhemi 3 Setup and Installation i E J Setup Gell ap ure o Select Components Which components should be installed Select the components you want to install clear the components you do not want to install Click Next when you are ready to continue 4 Mark the checkbox if you wish to create a desktop icon and click Next Select Additional Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing GelCapture then click Next Icons Create desktop icon 3 Setup and Ins
28. ence viewer If the image is very dark or very bright you may need to use the Dimmer dial on the front of the unit to attain more significant intensity results The WL image is captured continuously in live mode Reference Image Save 6 a Pee te 5 TO 3 x E e d q RS S y Eee a See Revert BN Copy Copy Inverted r lt 7 o gt ns gt i ELLA 1 LUNE A ADIEL A Le Target image adjustment Copy the Reference and press Done when finished E 4 Operating Instructions 8 Define your standards by dragging the mouse down to select the entire standard lane Reference Image Save gt wad waew men a o amp a u a 2 OD wo A ASES TA ana A quay Q k d aces cy E i E Bio lmagina Systems BENU i AAA tae ES ere ee AR PA AAA CI RR A de gt p e Copy the Reference and press Done when finished S wa White Light image displayed in the Save Image with Reference viewer ERRE pa A SE RA AA dd PRA e SA Cancel Revert S Copy Copy Inverted Z z q Original chemiluminescent iii image Target image adjustment E Threshold slider 9 Use the Copy button in the Save Image with Reference viewer to copy the standard in normal grey levels or Copy Inverted to invert the grey levels of the stand
29. erest 63 Installing Camera Drivers Windows 7 Professional 24 Camera Drivers Windows XP Pro 21 GelCapture for Windows 7 Professional 15 GelCapture for Windows XP Pro 13 Software Installation 12 M Maintenance 64 O Open An image 59 Camera 37 MicroChemi 36 P Preview 36 Previewing an Image 38 R Requirements Safety Requirements 11 S Saving 52 an Image 52 Options 53 Save Image with Reference 53 Setup Hardware 12
30. eries select any of the TIFF File Save Options and click Save Learn more about the Saving Options on page 56 4 Operating Instructions _ Live Off GelCapture File Edit Yiew Help DNR File Save Save in MicroChemi a c Fae My Recent Documents My Network File name Image v Places Save as type TIFF Files tif y Cancel TIFF File Save Options I Super resolution I Spot Filter Add Parameters To Image I Crop Image to view size Eram Ready b The capturing process will start and the images will be displayed in GelCapture s main viewing area You can abort the process by clicking the Abort button and only the images that were captured up to that point will be displayed Acquire image GelCapture EJ x File Edit view Help gt bed ME TH Vere QQne p nmary Display Acquisition Parameters Camera Exposure 0 0 1 0 Image tif Exposure Delay Binning 1 yas ol fee aha wre Fe 1 rh r 1 3 MIUMICS yikes Grey Level Count 19456 Edge 0 0 Peak 65535 Min 1988 Max 16639 Median 65535 Restore Original Contrast Stretching Set step size j 00 c After aborting the process you can click the Acquire button to start the process from the beginning using the values you already set This will overwrite the images that were already acquired in the aborted p
31. g Yes as described above the combined image will be displayed in the main viewing area File Edit View Help RHA bAi AQ ee Bo c cane ia tee eS QUERIOn Parameter ares ae tazas reams F Camera 5 oC Exposure tod C _ nes a 000 400 E mes o AS AO proa pmen Singe Image lt aa a Sua penea o Dile CS PA po a TSS Sy PS pee i HE tt TER E eee A a Standard Resolution X Sven Sar ao OE a moon _ A omp i a aamen AA _ lt _ eS se CD ED ap a qa a Querily Ez q aD Grey Level Count 412672 Edge 6730 Pesk 512 Mn 65 Max 610 Median 51581 Ma G Restore Original Contrast Stretching Set step size 100 4 Operating Instructions 13 A reminder to turn the illumination OFF will be displayed Please turn off the illumination Open an Image To Open an Image File 1 In GelCapture click the Open image Ey icon 2 Select the image to open from the DNR File Open dialog window Date modified No items match your search File name Files of type if Files Y 3 Click Open The selected image appears in the viewing area 4 Operating Instructions Image Statistics Imaging Tools GelCapture provides you with several imaging tools to alter the appearance of an image or gain more points of view of the raw image after it is captured For example it helps show very faint samples as well as to distinguish between c
32. he last image composite image 2 is The image is captured for three minutes capture for one minute and combined with image 2 to create image 3 Software Integration creates images that are made of a combination of previous and new information For example the second image in the above drawing is based on the information in both the first image and the second image Although the second image was captured with the same exposure time as the first image in the series 1 minute it provides illustrated information like that of an image that received 2 minutes exposure time combination of 1 minute 1 minute Hardware Integration creates images that are based only on new information For example the second image in the above drawing is based on brand new information which is collected following the capture of a new image with 2 minutes exposure time For capturing detailed information Hardware Integration is the preferred option 4 Operating Instructions To capture an Image Series using Software Integration From the Capture Type dropdown list select Image Series Click the green Ss Start button in GelCapture s main screen 1 2 3 The Image Series settings will be displayed on the left side of the screen 4 Use the Number of Images slider to select the required number of images 2 20 The Process Summary Table will be set according to the number of images selected for the series Live Off GelCapture Acquisit
33. ile Edit View Help A 3 M a lt P Return 5 Save y 1 Second 4 Operating Instructions Capturing an Image Series GelCapture provides you with two options for capturing an Image Series Software Integration and Hardware Integrations Software Integration creates a series of images using the same defined exposure time It merges captured images and displays the result of this merge Hardware Integration creates a series of images using different exposure times for each image It captures the image for the full amount of time specified The diagram below illustrates the differences between Software Integration and Hardware Integration using 1 2 and 3 minutes exposure times Software Integration Hardware Integration Image 1 Image 1 Image 1 exposure time B 1 minute captured image 1 minute exposure captured image 1 minute exposure The image is captured for one minute The image is captured for one minute Image 2 image 2 Image 2 2 minute exposure time captured image 1 P g captured image 2 minute exposure minute exposure captured image ae DOO The last image image 1 is captured for The image is captured for two minutes one minute and combined with image 1 to create image 2 Image 3 Image 3 Image 3 3 minute captured image 3 minute exposure captured image 1 minute exposure captured image 1 captured image 1 minute exposure minute exposure T
34. ion Parameters Sees File Edit View Help D EN F _ 4 rr bed Qe go ae Display I a O g Process Summary Table o Continue in Return Ready 5 Set the exposure time by adjusting the Hours Minutes and Seconds sliders 4 Operating Instructions 6 To change the Exposure time for a specific image left click the required cell in the Exposure column from the Process Summary table and a Set Time window will be displayed Set Time _ EHPosuwre ey 00 00 01 000 00 00 05 000 00 00 01 000 00 00 08 000 Ea 7 Adda time delay between images optional 7 1 Mark the Delay between images checkbox Br 7 2 The Set Delay window will be displayed where you can set the value for the time delay Set Delay 7 3 Click OK to save the Time Delay and close the Set Delay window 7 4 After you click OK you can change the Delay value of a specific image by left clicking the cell in the Delay column from the Process Summary table which will open the Set Delay time window 8 Select the type of display you require by choosing either Summary or Average these options are only available in Software Integration capture type sl 8 1 Summary the images are summarized by GelCapture see the Software Integration example in the table on page 49 a Choose Summary and click the continue Oconee button In the DNR File Save dialog enter the name and location for the Image S
35. ivity by four folds even though the background noise level remains similar to the level in a normal sized pixel A drawback to Binning is the loss of resolution In order to detect a given image with higher resolution you need to decrease the Binning parameters since as many pixels as possible must be detected separately The following example shows that in the first setting every pixel in the sensor is detected separately thereby providing a high resolution image 1 1 In the second setting every 4 adjacent pixels merge to form an image that is fourfold more sensitive than the first image while the resolution decreases by fourfold In the third setting every 9 adjacent pixels merge to form an image that is nine fold more sensitive than the first image while the resolution decreases by nine fold 4 Operating Instructions Original gi sa images Y Y Es Binned W i e Images Bin 1 Bin 2 Bin 3 Remember that higher Binning results in images of greater sensitivity but the resolution quality is compromised GelCapture lets you set the camera Binning Select an option from the combo box as seen in the following image Standard Resolution y JHigh Resolution Standard Resolution High Sensitivity Super Sensitivity Ultra Sensitivity s High Binning Super Binning Ultra Binning Max Binnine Quantity The option you choose will determine the type of Binning to be used When you select St
36. lose bands in the same image or helps discern an image that is unclear due to portions that are too dull or too dark Use the Inverted Image Pseudo color and Overexposure settings to assist you in examining the quality of the image before sending it for analysis When you use Imaging Tools to make changes and then save the image the image is saved with its original information without the changes made with the imaging tools However when the image is reopened in GelCapture the changes you made and saved are displayed For example if you invert an image and then save it the next time you open the image in GelCapture the image appears inverted However if you open the same inverted and saved image in other imaging analysis software the image appears as it did when you originally captured it i e not inverted Read the following section to learn more about Imaging Tools Image Statistics The Image Statistics Graph provides you with an illustrated version of the image data to help you capture images optimally This feature is very useful when you want to compare information between images by comparing the graph data or when you need specific statistical information of an image and in order to reveal very faint samples by using the stretching options When you detect an image the Image Statistics Graph is displayed on the left and other information is displayed below the graph Acquire image File Edit Miedo Help 2 bel e
37. marks of their respective holders This document may contain flaws omissions or typesetting errors no warranty is granted nor liability assumed in relation thereto unless specifically undertaken by DNR sales contract or order confirmation Information contained herein is periodically updated and changes will be incorporated into subsequent editions If you have encountered any error please contact DNR Contact us at 972 2 570 0818 USA Toll free number 1 866 300 4286 Email info dnr is com Table of Contents 1 troduction qa EO EE AS 4 2 TA E A 5 SO sacasssenaettnas seater tenaethecanseeeassanetansaaarenaesoars tees satamosssaeteeseanesesceenestace 5 Features ANG BON GINS cisietstcoscriicasseceraacsuoictassaisucosertesaveledeudesninteusucsuseuancietevsacsedeutestatautoassdevtosdacuitoutadsutosvedisoess eis 6 The MICFOCKH SMP UIE siccsciccvescveasvesssesaussewsna A vensenavoussesewenaueneveusuensvesualene ueseuonsVonsseseuesciees 6 TAC VICFOGI CII BOG A E 6 DETWET IO A A ed 7 GelCapturenn ina ia dans lan danna dan dans A 8 APOCO ION SE COCUL CS A O 9 A racial TA IN ETE NA E saa EO EE AA spd Gobo aiansaauiee baditida uadmegeldetdidadeeauane vines 10 3 Setup ANG Installation as 11 Safety Regule mentido 11 Electrical Rates ciavisavaacscaauscevecavivcseusvausvaravanauauaaacvieavoccvonevasesesavansvoujuiaananavauanaaeveusstsaVausueviuinsvius VaianauawauaNGaavenuee 11 Out OF TNE Bosnian idad ciar 11 Hardware Setup idad 12 Software lAstallati
38. n the Camera Calibration section on page 35 2 Cleaning the Drawer Tray and Unit a The Drawer Tray must be cleaned every time a sample is removed Use a soft non abrasive lint free cloth First dampen with water and then with 70 Ethanol b Toclean MicroChemi surface first use the cloth dampened with water and then dampened with 70 Ethanol c The MicroChemi should not come in contact with any harmful solvents 6 Troubleshooting Before you contact support it is recommended that you read over this section to see if you can resolve the problem by yourself To make this as easy as possible we have created a list of the most common problems and their possible solutions The MicroChemi doesn t work after turning the main power supply on Possible Reason Action A burnt safety fuse Replace the fuse 2 5 A for 220V systems and 4 A for 110V systems Problem with the camera illumination power supply Replacing the power supply The camera is not recognized Possible Reason Action Camera drivers installed unsuccessfully Uninstall GelCapture Reinstall GelCapture and the camera drivers Loose power supply cable Verify the wall socket and camera power supply connections Loose USB cable connections Verify the PC and MicroChemi USB connections Camera hardware problems Please contact your distributor Receiving a black image Possible Reason Action Short exposure time Increase the exposure time The iris is closed Please co
39. ntact your distributor Receiving out of focus images Possible Reason Action Focus settings were damaged Please contact your distributor MicroChemi operating temperature indicator stays red Possible Reason Action The MicroChemi camera ventilation holes are not Clean the MicroChemi ventilation holes clean Camera button is not activating Possible Reason Action Loose internal power supply button cables Please contact your distributor Button is receiving incorrect voltage Please contact your distributor 4 Troubleshooting Illumination button is not activating Possible Reason Loose internal power supply button cables Button is receiving incorrect voltage Dimmer button is not activating Possible Reason Loose internal power supply button cables Button is receiving incorrect voltage Improper illumination intensity Possible Reason Dimmer dial is set too low so there is not enough light Button is receiving incorrect voltage Low incoming LED voltage Faulty LED Dark box light penetration Possible Reason Improper positioning of the drawer Action Please contact your distributor Please contact your distributor Action Please contact your distributor Please contact your distributor Action Use the dimmer dial to increase the illumination Please contact your distributor Please contact your distributor Please contact your distributor Action Verify that th
40. oN iii a r e a a a toas ita 12 Installing GelCapture from CD Windows XP PIO ccccccsssseessecccccccneeeseeeesecscuussecessesasuueseeeesetsauausseeessssaaaneseeeeseneas 13 Installing GelCapture from CD Windows 7 Professional ooooccoooccncooonnononononononanononononononoconnnonnnnnnannononns 17 Installing the Camera Drivers Windows XP PLO cccccssesseccccsessecccnseeseccsnscsseccsnsecsesssseueessesseseesssseuseesssseeeesesseess 23 Installing the Camera Drivers Windows 7 Professional ooooonconnccncooonnononnnnnnnnonoconnonnnonononnonnonnnnonaroononos 26 A A R E 35 Calibrating the Camera from GelCapture s Menu baf cccccssseseccccnsesceccnsnssccccsueseecsssuensecsssauseessssauneeessaaanseeesas 38 4 Operatine INSTrUCTIONS ieissssesccavesncccawsecsccavaagvonsvansscaavenssdoaueesucusveasneus AEA 39 Turning the MicroChemi Unit On esesesessssesececscscsesecececessecececececsesecececscsesecececsesesesececscsesececececsesesececseseseseceee 39 Turning the MicroChemi Camera Ona ideada 40 inserting a Sample AA AAA n deine 40 Previewi ne an MaB cidade a 41 Capture IMAG OS ica a iio 43 EXPOSUTS TIMES 43 AUTOMAEICAEXDOS O IMC A A AA ia 44 Binin RESOIOTION YS SOON NASA aid 46 Capture Type Single IMage GNG Image EEE a 48 Saving Features aiii A NNE EAE NAN 55 TO SAVER O TO A AS A eo 55 SAVNO OPENS A A OT E ee T 56 SAVE Image with Reference iirinn ion 56 Operan Image air a vases A E A A AE E A E ees E S
41. orkgroup settings Computer name user PC Change settings Full computer name user PC pen Computer description Action Center Workgroup WORKGROUP Windows Update Performance Information and Tools AL You must activate today Activate Windows now Windows activation 5 Turn your MicroChemi unit ON The Device manager should recognize the newly connected device and alert you File Action View Help Es Bala 4 y user PC gt MM Computer gt Disk drives gt AE Display adapters gt i DVD CD ROM drives gt el Floppy disk drives b E Floppy drive controllers D 1 Human Interface Devices b 4g IDE ATA ATAPI controllers gt ED Keyboards b JI Mice and other pointing devices b i Monitors i Network adapters 4 l5 Other devices n MicroLine ML2020 Manager b JF Ports COM amp LPT gt E Processors Alert b a Sound video and game controllers gt Ml System devices p Universal Serial Bus controllers Device 28 3 Setup and Installation 6 Under Other devices right click MicroLine ML2020 and select Update Driver Software 4 3 user PC b gil Computer p Disk drives D A Display adapters gt 3 DVD CD ROM drives gt 28 Floppy disk drives p E Floppy drive controllers D 45 Human Interface Devices gt ca IDE ATA ATAPI controllers gt lt D Keyboards p F Mice and other pointing devices p Ml Monitors b Network adapters a gt Other devices E i y e Ports COM
42. re Time When you only roughly know the appropriate exposure time for your sample use the GelCapture Auto Exposure tool Using the Auto Exposure Tool 1 Set the Capture Type to Single Image Live Off GelCapture File Edit Yiew Help Reale Ween CD Preview Bi Auto hi Minute y RQ be ea Capture Type Single Image Resolution 1 Sensitivity Standard Resolution S Camera Ready Duantity Grey Level Count 0 Edge 0 0 Peak 0 Min 0 Max 0 Median O Contrast Stretching Set step size 100 Ready 4 Operating Instructions 2 Mark the Auto checkbox bed Qe go QD Preview Single Image y Resolution 1 Sensitivity Standard Resolution y Grey Level Count 0 Edge 0 0 Peak O Min 0 Max 0 Median O Contrast Stretching Set step size 100 Ready 3 Click the Start E button GelCapture is now evaluating the appropriate exposure time for you sample Please note that defining different Binning settings reflects on the Auto Exposure results higher binning will result in lower exposure time E Acquire image GelCapture File Edit View Help lel Bar QQ ee go ajajaj iv ax Binning 7 GelCapture Please wait CWAYWAVQAWANAVAVAVAVA AY Searching For Optimal Exposure Time 4 Once evaluation is completed a dialog box will be displayed asking what type of
43. res and Benefits The key features that make MicroChemi the choice for bio imaging professionals are e Quick 5 minute set up and convenient 1 button capture technology e Accurate high quality imaging for accurate quantification e Efficient optional work flow integration tools designed to boost efficiency e Chemiluminescent and bioluminescent gel documentation system e High resolution 2 0 Mp CCD Camera and 16 bit file format e Fixed lens iris and focus for high efficiency workflow e Enhanced sensitivity especially for faint sample detection e Advanced Dark Chamber technology providing clear images with minimum background noise e Intuitive software and hardware operation e Detailed sample viewing made available by real time high magnification e Click of a button software interface to perform most operations e Advanced software features which is designed especially for chemiluminescent use e g Save image with reference Auto exposure The MicroChemi Unit The MicroChemi is a sample imaging product which is easy and simple to use Read the following section to learn about the unit s parts and functionality The MicroChemi Body The MicroChemi body contains a high resolution camera to provide high quality imaging of biological samples The unit connects directly to the PC through a USB 2 0 compliant cable and to the wall through the power supply cable 2 Overview Drawer Tray The MicroChemi Drawer Tray is uniquel
44. rocess unless you change the Image Series name d When the capturing process is complete the Image Series will be displayed in GelCapture s viewing area You can toggle between single image view and multiple image view by clicking the icons at the top right side of the screen as illustrated below 4 Operating Instructions C Documents and SettingsWserWDesktopWicroChemi image5 tif GelCapture File Edit View Help Sy _ E F say a 1 21H Ge Pi ae Ane g Lad Display M Acquisition 3 27 233335 Parameters R E 5353 25S Camera s amp n BG Se Exposure 0 0 5 0 00 01 000 Image1 tif Image2 tif Image 3 tif 00 01 000 00 00 08 000 Binning 3333 E Imaged tif Images tif gt Acquire Return Quantity Grey Level Count 150528 Edge 122 1 Peak 65535 Min 9943 Max 16639 Median 43584 pa a E a Ma Restore Original Contrast Stretching Set step size 100 e Click Acquire if you want to recapture the series or click the Return Pte button to go back to the Image Series main screen 8 2 Average The shown images are the average of the entire Image Series When using the Average option the background noise is reduced Note When choosing Average all the images in the series MUST have the same exposure time a Define the exposure time and click Continue Oco The next steps are identical to steps a through e above
45. s Super resolution Spot Filter Add Parameters To Image Crop Image to view size Select from the following options e Save as type Select to save in either TIFF or BMP available only in single image capture mode e Super resolution Increases the image resolution by four fold by software e Spot Filter Decreases grainy gel phenomenon e Add Parameters To Image Adds camera detection parameters to the image e Crop Image to view size Allows you to save the image in the zoomed view size Save Image with Reference Often samples are dyed with more than one type of dye The ECL experiment examines biological samples which both emit light without an excitation source and have visible standard which is detected by white light illumination With the unique Save Image with Reference feature you can save one image of a multi dyed sample thus making your work more efficient A sample that needs detecting with two different illumination sources i e WL and CL creates two difficulties a How to detect a sample with two illumination types while using the same membrane and without touching or moving the membrane and b How to keep the two illumination types in the Same dynamic range To overcome these obstacles DNR uses a technology that combines the two illumination sources ECL and WL without moving or touching the membrane The images are rendered pixel by pixel while keeping the dynamic range First a CL image is captur
46. stall for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a a disk that contains the driver you want to install click Have Disk Show compatible hardware Model This driver is not digitally signed Tell river signing is 3 Setup and Installation 16 If the Windows Security window is displayed choose Install this driver software anyway O Windows secuiy TT ea x Windows can t verify the publisher of this driver software Don t install this driver software You should check your manufacturer s website for updated driver software for your device install this driver software anyway Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information 17 After successfully completing the driver update the successful update window will be displayed Click Close Windows has successfully updated your driver software Windows has finished installing the driver software for this device DNR Camera 18 Close the Device Manager and Control Panel windows The MicroChemi camera drivers are now installed and the camera is ready for use To activate GelCapture in Windows 7 Professional right click the GelCapture icon and Note a choose Run as administrator see the image on page 17 WWJ 3 Setup and Installation Camera Calibration After
47. stalled icons Click Finish to exit Setup 12 The GelCapture image capturing software for Windows 7 Professional is now installed on your computer To activate GelCapture in Windows 7 Professional right click the GelCapture icon and Note _ choose Run as administrator see the image on page 17 3 Setup and Installation Installing the Camera Drivers Windows XP Pro To Install the Camera Drivers Windows XP Pro 1 Turn the MicroChemi unit on Windows XP Pro automatically displays the Found New Hardware Wizard Choose No not this time and click Next Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software Yes this time only O Yes now and every time connect a device Click Next to continue 3 Setup and Installation 2 While the Wizard installs the drivers the following window will be displayed Found New Hardware Wizard Please wait while the wizard installs the software E DNA Camera 3 Ifthe Windows Logo testing window is displayed choose Continue Anyway Hardware installation The software you are installing for this hardware DNA Camera has not passed windows Logo testing to
48. tallation 5 Verify that the installation information is correct and click Install After you click Install the installation progress will be displayed a Setup SelCapture Ready to Install Setup is now ready to begin installing GelCapture on your computer Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Program Files DNR GelCapture Setup type MicroChemi Selected components GelCapture 6 software MicroChemi Additional tasks Icons Create desktop icon 6 Once installation is completed click Finish to exit Setup 5 Setup GelCapture lidad a Completing the GelCapture Setup Wizard Setup has finished installing GelCapture on your computer The application may be launched by selecting the installed icons Click Finish to exit Setup 3 Setup and Installation Installing GelCapture from CD Windows 7 Professional To install GelCapture Windows 7 Professional 1 Inthe CD right click the installation file GelCapture exe and select Run as administrator The drop down list will close after the selection is made Open Fy Run as administrator Troubleshoot compatibility Scan for Viruses Always available offline Restore previous versions Send to gt Cut Copy Create shortcut Delete Rename Properties 2 Inthe CD again locate the GelCapture exe file and do
49. the unit and software are set up the camera must be calibrated before it can be used Calibration must also be performed any time a different computer is connected to the MicroChemi unit There is a short camera calibration process of 135 minutes for images that require up to 30 minutes exposure time and a long camera calibration process of 8 hours for images that require exposure time of over 30 minutes It is recommended to perform both processes It is also advised to calibrate the system annually as part of preventive maintenance procedures You can either calibrate the camera immediately after set up and installation or at any other time when you open GelCapture Whenever you open GelCapture if you have not previously calibrated the camera the message in step 1 below will be displayed To Calibrate the Camera 1 Choose Yes to start the camera calibration process Note that if you click No calibration files will not be created images will include background noise and the Auto Exposure tool will not work properly For Best Results It s Recommended To Perform Camera Calibration Do You Want To Calibrate 2 When you click Yes to calibrate a dialog will be displayed asking you to either 2 1 Load the camera calibration files from the Camera Calibration CD supplied in the MicroChemi packaging OR 2 2 Create new camera calibration files Please insert the calibration files CD shiped with the system and click Yes To en
50. ting temperature Click to start capturing an image Provides an illustrated version of the image data to help you capture images optimally The captured image appears here 2 Overview Icons The most commonly used functions can easily be accessed with a single click of an icon Icon Name To gt Open Image Open a previously captured and saved TIFF image Ld Save Save the image Save Image with Combine an image of visible stained standards and Reference chemiluminescent bands PL Cony Copy the image to the clipboard You can then paste the image in a number of Windows applications m i Invert Image Display an inverse version of the image Ez Pseudo color Show the image in Pseudo colors Show Hide Over Show overexposed areas in red Exposure TE Zoom In Zoom into the image c Zoom Out Zoom out of the image Actual Pixels Display the actual image size ns Fit to Window Resize the image to fit in the viewing area a Sereen Display the image on the entire screen To return to GelCapture s main screen press Esc I ka ml Region of Interest Select a specific area to work within the image 3 Setup and Installation This chapter explains all the steps required for proper installation of your system and includes safety requirements PC requirements software installation and more Once you have completed these steps your MicroChemi should be fully operational Safety Requirements e Ensure the AC power ou
51. tlet is properly grounded to protect both the personnel and the equipment e Verify that the device turned off before making any connections e Do not block the ventilation openings of the MicroChemi unit e Place the MicroChemi unit at least 30 cm 12 inches away from walls and ceiling e Do not store below 10 C The recommended operating conditions for MicroChemi are 25 C 78 F and 55 relative humidity Maximum ambient temperature 28 c maximum ambient humidity 80 e Do not store the system in direct sunlight or in the direct flow of the air conditioner e Donotclean the system with harmful solvents Only use a soft cloth dampened with water and then with 70 Ethanol e Intended for indoor use only e Do not open the unit Due to the danger of exposure to high voltage only trained service technicians should open the unit e Do not place the system near any motorized vibrating or magnetic equipment e Unit protection can be impaired if used in a manner not specified by the manufacturer Electrical Ratings The system is compatible with 100V and 240V Out of the Box Your MicroChemi unit packaging should include the following items MicroChemi unit dimensions W 35 x L 32 x H 64 cm Pre connected USB 2 0 camera cable AC power cord European N America compatible GelCapture CD image analysis software CD and Calibration Files CD also includes this User Manual and Quick Reference Guide AU Ne MicroCherni User Manual
52. ty in order to attain the best results The following chapter provides an explanation on how to set the different parameters Exposure Time Exposure time defines the period of time during which the camera collects photons of light that emit from the sample Therefore increased exposure time means more time for light to reach the camera sensor to display sections of the sample that were not visible with a lower exposure time To Set the Exposure Time 1 Click the Exposure Time combo box and choose a time value type Seconds Minutes or Hours or a preset exposure time as displayed in the following images File Edit View Help File Edit View Help gt bel GR GS Bas 0 bed AD GS ii Q Preview CD Preview si a 30 Seconds v 30 Seconds v Seconds Minutes Hours Choose value type 1 2 Second 1 Second 5 Seconds 10 Seconds 30 Seconds Preset time values 1 Minute 1412 Minutes 5 Minutes 10 Minutes Minute 2 Minutes 4 Operating Instructions 2 Ifyou want to set the time value manually choose either Seconds Minutes or Hours and the window for entering a value will be displayed Insert the preferred time value to set the Exposure Eo lt A Fie Edt View Hep Bi pat Mew Be i Se AE Exposure Auto ET O e z AAA Standad Resobsion Standard Resolution CameraReady EX Ga time accordingly n Automatic Exposu
53. uble click it to start the installation 3 Inthe next window select Allow trust this program know where it s from or I ve used it before to allow the program to run 3 Setup and Installation 4 Click Next 18 Setup GelCapture nl XA y a Welcome to the GelCapture Setup Wizard This will install GelCapture 6 0 on your computer lt is recommended that you close all other applications before continuing Click Next to continue or Cancel to exit Setup Select Destination Location Where should GelCapture be installed A Setup will install GelCapture into the following folder To continue click Next f you would like to select a different folder click Browse At least 43 9 MB of free disk space is required 3 Setup and Installation 6 Click the combo box and select the MicroChemi component from the list Select Components Which components should be installed Select the components you want to install clear the components you do not want to install Click Next when you are ready to continue Which components should be installed Select the components you want to install clear the components you do not want to install Click Next when you are ready to continue MicroBIS MiniBIS MiniBIS Pro Mini Lumi LumiBIS LumiBIS with HeptaLED ChemiBIS 0 5M F ChemiBlS 0 5M F ChemiBIS 0 5M with HeptaLED MChemiBiS MF ChemiBlS MF ChemiBIS with HeptaLED FChemiBIS M BIS M with HeptaLED
54. utomatically be marked Click Acquire to continue CCD Calibration DPS SUS nA art p i AA Short 135 minutes v TEM ek 3 we ILLIA LS AA A E 7 The CCD Calibration Progress window appears and displays the Dark images and Bias images Calibration progress while the system creates reference pictures for the camera CCD Calibration Progress 8 After camera calibration is completed successfully a message box will inform you that Calibration files have been created successfully Calibration files have been created successfully 3 Setup and Installation Calibrating the Camera from GelCapture s Menu bar If you did not initially perform Camera Calibration you can activate it at any time from GelCapture s menu bar The steps to take are identical to the calibration steps described above except for the manner of activating the process 1 From the File menu in GelCapture select Camera Calibration Live Off GelCapture ES Edit View Help Open Tiff Image Print Ctrl P Print Preview Print Setup Camera Calibration 2 Follow Calibration steps 2 to 8 on page 35 4 Operating Instructions The MicroChemi and GelCapture system lets you configure and fine tune various features to optimally detect different sample types The following chapter provides stepwise explanations on how to operate the system from basic to advance The following workflow allows you to easily capture and save
55. y designed to facilitate sample image analysis The samples are placed on the Drawer Tray which is modeled with a bordered niche to ensure the sample solvents will not to leak out onto the Drawer Tray itself Drawer Tray 2 Overview GelCapture The GelCapture application seamlessly integrates with the MicroChemi unit thereby giving you complete control of configuration capturing and saving sample images on your PC for analysis The friendly GUI lets you perform common actions with a single click of a button or icon The following image displays the application s main screen Menu Bar _____ e pr ue iw Icons Toolbar _____ P H En w ea eo E Preview ____ Exposure Time ______ Capture Type ______ Binning ____ _ Camera Ready Indicator ______p Viewing Area 2 Overview Application Features Menu Bar Icons Toolbar Preview Exposure Time Capture Type Binning Camera Ready Indicator Start Image Statistics Viewing Area The drop down menus contain all of the software s features Commonly used functions can be triggered with a click of an icon from the top toolbar Preview the image to make sure it is positioned properly Sets the exposure time Select either a Single Image or an Image Series for capturing Set the camera Resolution and Sensitivity The indicator light changes from red to green when the camera reaches opera

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