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1. 37 0 556 Time of platform heating from 25 C to 37 C 6 min Speed control range 250 1 400 rpm Orbit 2mm Display LCD 16x 2 signs Digital time setting 1 min 96 hrs 1 min increment Overall dimensions WxDxH 240 x 260 x 160 mm Weight 5 1 kg Input current power consumption 12V 4 8A 58W External power supply Input AC 100 240 V 50 60 Hz Immunochemistry for immunofermentative Output DC 12V reactions For B 2E thermoblock Biochemistry for enzyme and protein analysis ORDERING INFORMATION Cat number Molecular biology for nucleic acid extraction TS DW without block BS 010159 A02 Interchangeable Blocks Cat number ap B 2E Block for one deep well plate Eppendorf 96 1000 ul BS 010159 AK e B 2S Block for one deep well plate Sarstedt Megablock 96 2200 ul BS 010159 CK 2 Block for one deep well plate Porvair 96 2000 ul BS 010159 EK o B 2A Block for one deep well plate Axygen 96 2200 ul BS 010159 FK Other Blocks please inquire NOILdld5Ds3G P 5 1 21412345 P CATALOGUE 2016 SHAKER INCUBATORS PERSONAL BIOREACTORS Shaker Incubators Personal bioreactors ES 20 Orbital Shaker Incubator ES 20 with platform UP 12 A The ES 20 is a compact bench top Shaker Incubator used for mixing of biological liquids as well as for incubation and ZZ cultivation of biological liquids according to the operator O set program I Built in micropr
2. Wal AS ml 165 bk tOr 370 PH 10m 000 Medical Biological Research amp Technologies cm MA 1 Mini Rocker WELCOME TO BIOSAN PRODUCT CATALOGUE 2015 2016 Our mission is the development manufacture and supply of innovative laboratory instruments developed on the base of the uppermost advanced scientific and manufacturing technologies For more than 23 years Biosan has been a reliable partner for everyone who chooses equipment for working in the fields of biomedical diagnostics biotechnology and Life sciences Biosan catalogue 2015 2016 presents devices for mixing vortexing centrifugation incubation cultivation water purification systems DNA RNA UV cleaner boxes devices ensuring biosafety of laboratory air as well as analytical solutions for bioprocesses SOLUTIONS FOR SAMPLE PREPARATION Biosan Research amp Development team is constantly focused on solving irreproducibility problems of experimental data that is usually caused by the complexity of biological material sample preparation The lack of strict regulation in sample preparation determines the most errors during the reproduction of the method Errors accumulate due to the decrease of the reactant s volume the absence of intermediate temperature logistics of mixing the lack of air decontamination systems in laboratories during the working process and no regulations for cellular material storage We offer new developments on
3. ACE 5 amp riy L T W 7 r cr FK 1 p 4 A 5 edi f T z E f Md 7 3 4 s gt f a 4 5 e j 4_ i z AT Pv m ws g 0098 zd 4 is s 2 T aT P w E w 4 5 Tw 1 LJ E D Iu Pai To ve vd xw 1 Tre ma o a 1 r NC P Pu e aP b y oe n E Fr t c 1 F giu as 2 A y Lo Ne ly j ST z 8 y r rsg x d I lt a E goa 9 F a 11 s 4 4 YA x z d 4 E LE kal i m i i I 1 4 To a T m J g 4 v V uS d uf J 1 ier L 2 P1 Ju P A 2 4 v f sii i a e ways f 2 gt 4 a L L ua t r A CU gu At d LT i 4 A i bia 1 m CATALOGUE 2015 2016 THERMOSTATIC EQUIPMENT WATER BATHS ORBITAL LINEAR SHAKING BATHS UNSTIRRED WATER BATHS HEATING COOLING CIRCULATORS SPECIFICATIONS DESCRIPTION Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators WB 4MS Stirred water bath Water bath thermostat WB 4MS is designed for chemical pharmaceutical medical and
4. 16 8 8 sockets and PL 1 platform for mixing single tube up to 15 ml An optional 6 socket platform PV 6 10 for 10 ml tubes max tube diameter 15 mm can be supplied on request Speed control range 500 3 000 rpm Acceleration time 3 sec Orbit 2mm Continuous impulse operation Maximum load 70 gr Maximum continuous operation time 8 hrs Dimensions 120x180x100 mm Weight 1 5 kg Input current power consumption 12V 320 MA 3 8 W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V ORDERING INFORMATION Cat number V 32 with standard platforms PL 1and PV 32 5 010207 Optional platforms PV 6 10 optional platform BS 010207 BK PV 48 optional platform BS 010207 GK Platform PV 6 10 Platform PL 1 Platform PV 32 Multi Vortex V 32 AQ ele cs Speed biotan 94 MSV 3500 with platform SV 8 15 Multi Speed Vorte MSV IKO nd peed biofan MSV 3500 with platform SV 4 30 Optional platforms sv 16 8 sv 10 10 sv 8 15 sv 4 30 sv 16 8 Devices Rockers Shakers Rotators Vortexes MSV 3500 Multi Speed Vortex Platform for 16 1 5 ml 8x0 5 ml 8x0 2 ml microtubes 11 8 6 mm Platform for 10x 10 ml tubes 12 mm diameter Platform for 8x 15 ml tubes 16 mm diameter Platform for 4x 50 ml tubes 30 mm diameter sv i0 10 Multi Speed Vortex MSV 3500 is designed for soft or intensive mixing of reagent
5. Compact CO2 Incubator Centrifuge vortex for PCR plates CHECK OUR WEBSITE CVP 2 is a fully automatic device for reproducing FORTHE LATESTS UPDATES sms algorithm for 2 PCR plates at the same time thus saving time considerably A must have instrument for PCR and DNA analyses laboratory S Bt Smart Biotherm provides a six sided direct heating of chamber walls and a door thereby CVP 2 is 4 devices combined in 1 ensuring uniform distribution of temperature and 1 Centrifuge Maximum RCF 200 x g 1 500 rpm CO2 within the incubator 2 Vortex 300 1 200 rpm Vortexing regulation Built in high precision infrared CO2 sensor allows timer 0 60 sec to measure and control the level of CO2 The sensor is resistant to changes in temperature and humidity inside the incubator Stainless steel chamber is securely sealed to mini mize contamination and facilitate cleaning S Bt is equipped with a UV air recirculation system 1 UV lamp and a fan UV air recirculation system is mounted behind the rear wall of the incubator and provides decontamination of the working space In the wall of S Bt there is a convenient access port that can be used for variety of sensors and wires of devices installed in the incubator 3 Centrifuge Vortex 4 SMS cycler for realization of the sms algorithm Smart Biotherm NEW ULTRAPURE WATER SYSTEMS Labaqua Series bofan Labaqua Bio e Labaqua HPLC produces water with very low organic
6. 0 3 OD Light source NIR Light diode Measurement periodicity per hour 1 60 Cultural media volume 10 30 ml Type of tube for aerobic and anaerobic cultivation 50 ml tube with membrane filter TubeSpin Bioreactor 50 TPP 25 70 5 C above ambient 70 C Temperature setting range for RTS 1 heating only Temperature setting range 4 C 70 for RTS 1C with cooling 15 C below ambient 70 C Stability 0 1 C Speed control range 50 2 000 rpm Max number of units connected to the software 12 recommended LCD Minimum PC requirements Intel AMD Processor 1 GB RAM Windows XP Vista 7 8 8 1 2 0 USB port Optimal PC requirements Intel AMD Processor 3 GB RAM Windows XP Vista 7 8 8 1 2 0 USB port 130x212x 200 mm 1 5 kg 12V DC 3 3 A 40W Input AC 100 240 V 50 60 Hz Output DC 12V Display Overall dimensions WxDxH Weight Input current power consumption External power supply FEATURES Innovative Mixing Technology Reverse Spin RTS 1 and RTS 1C are personal bioreactors which provide Reverse Spin type of agitation and logging of microbial growth in 50 ml tubes in real time See the Reverse Spin Technology Innovative Principle of Microbial Cultiva tion on page 110 e Innovative mixing due to reverse spinning of the sample around its own axis e Due to innovative mixing technology it is possible to measure optical density of the probe in real time
7. DESCRIPTION DNA RNA UV Cleaner Boxes DNA RNA UV cleaner boxes UVC T AR UVC T M AR UVT B AR and UVT S AR are designed for clean operations with DNA samples They provide protection against contamination All models are bench top type made of metal framework glass or plexiglas walls and working surface painted with powder enamel or made of stainless steel See the specifications table on the page 88 UV cleaner boxes are equipped with an open UV lamp installed in the upper hood UV radiation from the open lamps disinfects the working area inactivating DNA RNA fragments during 15 30 min of exposure A digital timer controls duration of the direct UV irradiation A daylight lamp provides proper illumination of the working surface UV cleaner boxes are equipped with a flow type bactericidal UV cleaner recirculator AR which provides constant decontamination inside the box during operation They are recommended for opera tions with DNA RNA amplicons UV cleaner recirculator AR consists of a UV lamp a fan and dust filters organized in a special body so that a user working with a UV cleaner box is protected against UV light Recirculator increases the maximum density of UV light making it suffi ciently effective for DNA RNA inactivation The UV recirculator processes 100 UV cleaner box volumes per hour creating permanent aseptic conditions of operation inside the UV cleaner box Specially assigned mobile tables wi
8. Manufacturers Corning Nunc etc Rack for rotors RR U ORDERING INFORMATION Cat number R 12 15 Swing out rotor for conical bottom centrifuge tube BS 010208 EK Minicentrifuges Vortexes Mini centrifuge Centrifuges Interchangeable Rotors and Accessories for LMC 3000 and LMC 4200R R 6 amp R 6P ROTORS FOR PLASTIC TUBES WITH CONICAL BOTTOM AND A LID Rack for rotors RR U Results after 1 500 rpm spin for 9 minutes using Grifols Lateral Gel Cards 1 6 in ELI C HHM 1600 Rotor R 6 Rotor R 6P Tube holders material Aluminium POM Kocetal Rotor type Swing out Dimensions x length 29x115mm Capacity 6 Tube volume 50 ml Max speed 4200 rpm Max temperature 150 C Max RF aoe Tube Manufacturers ram Grener Bone ORDERING INFORMATION R 6 rotors for plastic tubes with conical R 6P bottom and a lid Cat number BS 010208 DK BS 010208 XK RR U RACK FOR ROTORS ORDERING INFORMATION RR U Rack for rotors Cat number BS 010208 UK R 24GC ROTOR FOR GEL CARDS R 24GC Rotor for Gel Cards for blood group serology testing Forward Group Reverse Group RhD Type and 3 cell antibody screen Maximum speed 1 500 rpm Centrifugation Time 9 minutes Capacity 24 Gel Cards Dimensions of a gel card 1 xw 53 x 74mm Gel card Manufacturers Grifols DiaMed etc Rotor type Swing out ORDERING INFORMATION Cat number R 24GC Rotor for Gel Cards BS 010208 V
9. SAP2S SAP2 SAP12 18 6 SAP34 SAPD WB5 WB12 WB18 WB26 WB34 X WBDUAL PLUS PLUS PLUS PLUS PLUS PLUS PLUS zie ul 23 9 _ A Fo m 4 4 4 4 4 3kg 3kg 3 kg 6 kg 9 5 kg 9 kg 14 5 kg 9 kg 200 mm l 215mm 1 215 I 380 mm 590mm 590 mm 770 mm I 380 mm 185mm 335 mm 335 360 mm 335 335 mm 335 545 mm h 200 mm h 150mm 200 225 mm h 275mm h 275mm h 370mm h 225 mm Tank capacity Di ne 5L 12L 18L 26L 34L 5L amp 12L shallow _ Temperature range ambient t C 5 to 99 Temp display and setting o 0 1 C resolution _ stability DIN 12876 0 2 70 Temperature setting energy digital regulation NEN m User adjustable over temp alarm Fixed thermal cut out Dry start boil dry protection Hu Programmable temp presets 3 Countdown timer with audible alarm 131 281 or Working volume l w mm 117x131 139x289 131x281 281x306 485x281 481x278 635 x 281 Suis Drain tap included d B Heater power 120V 230V kW 0 25 0 25 035 035 0 35 0 35 08 08 14 105 14 105 18 13 115 115 Supply voltage V 120 or 230 CE and CSA approved ORDERING INFORMATION catalogue number matches the name of the product Thermostatic equipment Water Baths Orbital linear shaking Baths 68 Unstirred Water
10. Small HEPA filter Large HEPA filter open UV lamp Fluorescent lamps ORDERING INFORMATION Cat number TLC S Thermostated Laminar Flow Cabinet BS 040114 AAA TTL S Moving table for the cabinet BS 040114 AK Optional accessories TTL S Moving table for the cabinet Dimensions Ix w xh 800 x 750 x 770 mm Load up to 200 kg Consumables Name Description Small HEPA filter H14 class 305x305x68 mm Large HEPA filter H14 class 457x610x68 mm UV lamp TUV 25 W T8 G13 Fluorescent lamp 24 W 5 G5 Fuse M 8 0 A Thermostat Cat number BS 040114 S28 BS 040114 S29 BS 040114 S18 BS 040114 S19 BS 040114 S27 CATALOGUE 2015 2016 PHOTOMETERS Photometers DEN 1 and DEN 1B McFarland Densitometers Densitometers are designed for measurement of cell suspension s turbidity in the range DEN 1 0 3 5 0 McFarland units 100 x 106 150 x 107 cells ml DEN 1B DEN 1B 0 0 6 0 McFarland units 0 180 x 107 cells ml Densitometers provide the opportunity to measure solu tion turbidity in a wider range up to 15 0 McFarland units however it is necessary to remember that in this case the standard deviation values increase DESCRIPTION A densitometer is used for measurement of cell concen tration bacterial yeast cells during fermentation process determination of microorganism sensitivity to antibiotics microorganism identification using various test syst
11. 4 C to 40 C eo Product video is available on the website Platform for semi unskirted PCR plate 200 ul Platform for 24 tubes 1 5 2 ml Platform for 32 tubes 0 5 ml Vortexing a 50 ml tube ji Vortexing a 15 ml tube APPLICATIONS OF UNIVERSAL BUILD IN PLATFORM Devices Rockers Shakers Rotators Vortexes MPS 1 Multi Plate Shaker Mixing Speed control range Platform options For semi unskirted PCR plate or 96 microtest tubes 0 2 ml For 24 microtest tubes 1 5 2 ml For 32 microtest tubes 0 5 ml For 24 microtest tubes 0 5 ml and 48 microtest tubes 0 2 ml 300 3 200 rpm 100 rpm increment P 02 96 P 2 24 P 05 32 P 02 05 Universal platform for deepwell plates 96 well microtest plates U V or flat bottomed 384 well microtest plates Types of mixing presets VORTEX HARD MEDIUM SOFT CUSTOM Features a Pulse Mode mixing function Features a Vortex function Maximum load Mixing Orbit Acceleration time to maximum speed Digital time setting Maximum continuous operation time Noise level not more Weight Overall dimensions W x D x H Input current power consumption External power supply Deepwell plate 96 1000 ul ORDERING INFORMATION MPS 1 Multi Plate Shaker with built in universal platform Microtest plate 200 ul 3 200 rpm 2 600 rpm 1 800 rpm 1 000 rpm adjustable rpm 0 3 kg 3mm 5 sec 0 60 min 15 sec increment non stop 8 h
12. B B stainless steel sheath 3 mm x 30 mm long with 350 mm of cable QBEP WM Short form probe For in sample or in block temperature control encased in stainless steel sheath 3mm 14 long with 350 mm of cable Microplate blocks of molecular biology and biotechnology applications Double size blocks 140 x 100 x 75 mm supplied with additional extraction tool 96 holes in microplate configuration for 0 2 ml microplates strips or indi vidual tubes Uniformity 0 3 C with in tubes across the block 6 2 mm holes 14 mm hole depth Universal block for standard 96 well plates u well v well flatm bottom high temperature Uniformity 0 5 C between wells supplied with hinged double layer lid to create an insulated incubation chamber Safety covers not required with QDP FL Microtiter blocks Made from tough clear acrylic for maximum visibility whilst preventing accidental touching of a hot block QBL1 QBL2 QBL4 QBL2 QBL1 QBL2 or contamination of samples from splashes Clearance height 85 mm b T 8 a FJ A VW A 2 1 NM Ag a r My 4 3 ee i L as 5 i 4 e M a C phe MP1s s y w ru 5 r i a aes Ye ww f tx POTERO NO v D A X y ad 2 e Y E m is y 4 a
13. C from cold rooms to incubators at maximal relative humidity 8096 CATALOGUE NUMBER Cat number DESCRIPTION MSH 300 with stand BS 010302 OAA Optional accessories SKM2 Clamp BS 010309 AK DPMD Double clamp BS 010309 CK Intelli Stirrer MSH 300i is a digital version of magnetic stirrer with heating it is designed for laboratories with higher requirements It offers digital setting and control of tempera ture and rotation speed A powerful magnet allows mixing solutions with glycerine viscosity level Maximum volume of stirred liquid water is 20 litres An external probe provides direct control of the stirred liquids temperature EXTERNAL TEMPERATURE PROBE Probe type Thermocouple Connection type K A cable is covered with Teflon mechanically strong elastic and chemically stable against oils acids aggressive reagents and liquids Cable length 1m Operation temperature range 50 C to 250 C CATALOGUE NUMBER Cat number Intelli Stirrer MSH 300i with stand BS 010309 AAA Optional accessories External temperature probe BS 010309 BK SKM2 Clamp BS 010309 AK DPMD Double clamp BS 010309 CK MSH 300 with a stand eo un Product video is available on the website Intelli Stirrer MSH 300i with a stand and external probe SKM2 DPMD clamps netic Stirrers Overhead Stirrer MSH 300 and Intelli Stirrer MSH 300i Speed control range Max stirring volume Plate t
14. Dimensions 285 x 220 x 40 mm 255X255 220 X 220 mm 250X190 mm Working Area 270 x 195 x 40 mm 230 230 mm 215 215 250 190 Cat Number BS 010108 AK BS 010116 AK BS 010108 BK BS 010108 EK P 6 250 Used on PSU 10i ES 20 84 Platform with clamps for flasks 250 300 ml 6 places 250X190 mm 250X190 mm BS 010108 DK P 16 88 Used on PSU 10i ES 20 Platform with spring holders for up to 88 tubes up to 30 mm diameter 9 10 ml 15 ml 50 ml tubes 275X205X75 mm 275 205X75 mm BS 010116 BK Platform uP 330 Used on PSU 20i P 30 100 Used on PSU 20i ES 20 60 16 250 Used on PSU 20i ES 20 60 p 9 500 Used on PSU 20i ES 20 60 P 6 1000 Used on PSU 20i ES 20 60 PP 400 Used on ES 20 60 uP 168 Used on PSU 20i gt ES 20 60 FC 100 Fc 250 Fc 500 FC 1000 QD pp 20 4 Used on PSU 20i QD pp 20 3 Used on PSU 20i QD pp 20 2 Used on PSU 20i pp 20 Used on PSU 200 Devices Rockers Shakers Rotators Vortexes Description Universal platform with adjustable bars for different types of flasks beakers Platform with 30 clamps for 100 ml flasks Platform with 16 clamps for 250 ml flasks Platform with 9 clamps for 500 ml flasks Platform with 6 clamps for 1000 ml flasks Flat platform w
15. SWING OUT ROTOR FOR STANDARD 96 WELL MICROTITRE PLATES Rotor type Swing out Dimensions x 128 x 85 6 Max height up to 45 mm Capacity 2 Max speed 2 000 rpm Max RCF LMC 3000 560xg LMC 4200R 560xg Plate Nunc Greiner Sarstedt Corning Manufacturers Greiner Bio one etc ORDERING INFORMATION Cat number R 2 rotor BS 010208 AK Additional adapter for R 2 N EW A PTE R Set of 2 adapters made of Ertacetal C Autoclavable R 12 10 SWING OUT ROTOR FOR ROUND BOTTOM CENTRIFUGE TUBE Rotor type Swing out Dimensions O x length 16x 105 mm 12 Tube s volume 10 15 ml Max speed 4 200 rpm Max RCF LMC 3000 1 700xg LMC 4200R 3 370 Tube Nunc Greiner Greiner Bio one etc Manufacturers Rack for rotors RR U ORDERING INFORMATION Cat number R 12 10 Swing out rotor for round bottom centrifuge tube BS 010208 BK Additional adapters for R 12 10 Dimensions O x length BN 13 75 for vacutainers 2 5 ml 13x75mm BS 010208 PK BN 13 100 for vacutainers 4 8 ml 13 100 mm BS 010208 QK 16 100 for vacutainers 8 9 ml 16 x 100 mm BS 010208 RK Set of 12 adapters made of POM C polyacetal Max temperature 100 C R 12 15 SWING OUT ROTOR FOR CONICAL BOTTOM CENTRIFUGE TUBE Rotor type Swing out Dimensions x length 17x120mm Capacity 12 Tube volume 15 ml Max speed 4 200 rpm Max RCF LMC 3000 1 700xg LMC 4200R 3 370xg Tube Falcon Greiner bio one Sarstedt
16. consumption The use of direct drive and brushless motor allows continuous mixing up to 7 days and ensures reliable trouble free operation for more than 2 years Non slip temperature resistant silicone mat located on the shaker s platform provides stable position for vessels during shaking The platform is suitable for placing a versatile dimpled PDM mat for different size tubes Mini Shaker can be used in cold rooms or incuba tors operating at ambient temperature range 4 C to 40 C Speed control range 5 60 rpm Fixed tilt angle 7 Direct drive mechanism Max continuous operation time 168 hrs Maximum load 1kg Platform working area 215x 215mm Overall dimensions 235 x 235 x 140 mm WxDxH with platform installed Weight 1 2 kg Input current power 12 V 260 mA 3 1 W consumption External power supply Input AC 100 240 V 50 60 Hz Output DC 12 V ACCESSORIES FOR THE STANDARD PLATFORM Optional dimpled mat PDM prevents different size tubes from rolling around the platform ORDERING INFORMATION Cat number 3D BS 010151 AAG with standard platform Bio PP 4S Optional accessories PDM dimpled mat PDM eo Product video is available on the website 3D with a gel tank PDM dimpled mat 3D uni rotation Devices Rockers Shakers Rotators Vortexes Multi Bio 3D Mini Shaker Programmable mini shaker Multi Bio 3D is designed for a variety of applications hybridization reactions cell
17. lt Applications Agglutination NEW e Extraction Multi Bio 3D EZ Rp e Gel staining J destaining oe P TS 100 with heating TS 100C with heating and cooling gt V 32 ar MR 12 Applications e Agglutination dh 4 ce e Blot hybridisation a e Gel staining destaining Level of liquid 10 107 ml 10 ml 10 10 ml Erlenmeyer flasks and Petri dishes vacutainers PCR plates microtest plates Cultivation flasks and tubes up to 50 ml and Eppendorf type tubes 128 Product Line Examples a 8 Y A lt 2 lt Un U 5 cc a cc O a a z lt 2 v lt cc gt lt LLI lt Z cc i lt 2 gt O O 2 T U LLI Centrifugation for DNA Extraction Centrifugation for DNA Extraction in plates Sample preparation Smooth resuspending and centrifugation Sample preparation in UV Cabinet for PCR Sample Thermostating and Incubation Amplification Fluorescence End Point Analysis Electrophoresis Real time Amplification Q Microspin 12 y page 47 LMC 3000 page 48 MSC 6000 or similar see Multi Spins page 44 page 44 c eet and Combi Spins page 43 UVC T M AR or similar see UV Cabinet page 86 for PCR page 87 0 was TDB 120 TS 100C pe page 55 page 31 i Thermocyclic amplificator
18. short time under timer control and continuous operation e Indication of UV lamps working hours TE TROC e Convenient fixation on walls standard e Mounting on a movable tripod optional O UVR M UVR Mi UVR Mi O Tripod UVR S UVR Mi UVRM UV Radiation level in mW cm sec Vegetative Bacteria Viruses UVR M and UVR Mi UV Cleaner Recirculators Operation principle BEFORE thymine formations C M dimers ORDERING INFORMATION UVR M N T dimers UVR Mi Optional adapters UVR S tripod Specifications UVR M UVR Mi UV radiation source 25W Bactericidal i nns 2 lamps Philips TUV25WG13 UV C UV radiation level 18 mW cm sec 36 mW cm sec Air flow productivity 14 m hour Full user protection from direct UV light Yes Display LCD UV lamp operation indicator Yes Yes UV lamp lifetime counter No Yes Timer 1 min 24 hrs non stop Automatic switch ON OFF Yes Lamp fault detection Yes Overall dimensions WxDxH 110x135x660 mm 110 130 660 mm Weight 3 4kg 3 4kg Nominal operating voltage 230 V 50 Hz or 120 V 60 Hz 230 V 50 Hz Power consumption 230 120 V 125 VA 540 mA 160 VA 1 3 A 110 W 0 5 A BS 040105 AAA BS 040110 AAA BS 040105 AK See UVR M and UVR Mi UV air flow Cleaner Recirculators Test Report on page 123 BEFORE AFTER Cat number 5 1 21412345 P
19. 0 05 Distance from UV lamp to recirculator s walls 126 Applications Articles Sensitivity of microorganisms to UV radiation intensity in UV air recirculators UVR M and UVR Mi UVR Mi UVRM UV Radiation level in mW cm2 sec Vegetative Bacteria Viruses Microorganism examples Yeast Viruses Vegetative Bacteria Clostridium tetani Saccharomyces Bacteriophage E coli cerevisiae Mycobacterium tuberculosis Influenza Brewer s yeast Salmonella Dysentery bacilli Staphylococcus aureus Streptococcus hemolyticus BEFORE AFTER bio Applications Articles How to Choose a Proper Shaker Rocker Vortex C VA 2 vr Multi Bio RS 24 C PST 60HL 4 PST 100HL c Applications with heating with heating ES 20 60 with heating PSU 20i Multi RS 60 Microbiology m eo Extraction Bio RS 24 Cell growing vi e Luo f Applications 1 Microbiology p PST 60HL e Extraction 4 d with heating V 1 a amp hy e Cell growing 7 NEW RTS 1C amp RTS 1C PSU 10i Applications e ELISA analysis uel Cam Omm Hybridization a _ MSV 3500 PSU 2T ES 20 with heating Applications MPS 1 T A analvsi oor 25 DNA analysis 9 ema Genome sequence MR 1
20. AND LATEST UPDATES WILL BE PUBLISHED ON OUR WEBSITE WWW BIOSAN LV FILTER SPECIFICATIONS Installed HEPA filter class H14 according to EN 1822 1 2009 Efficiency of HEPA filters 99 995 HEPA filter dimensions main 457x610x68 mm additional 305x305x68 mm Downflow air speed range 0 25 0 50 m s in Standard mode Recirculation level in the cabinet 70 Noise level front sash raised lt 60 dBA front sash closed lt 50 dBA TEMPERATURE SPECIFICATIONS Setting range 25 C to 37 C Regulation range 5 C above RT to 37 C Stability at 37 C t15 C GENERAL SPECIFICATIONS Bactericidal UV lamp in the cabinet 1xTUV 25BT T8 G13 Bactericidal UV lamp in the recirculator 4xTUV 25BT T8 G13 UV radiation type UV 254 nm w o ozone Average UV lamp life time 9000 h Digital time setting 1 min 96 h 59 min Luminiscent lamps 2x24W 830 5 G5 Work area luminosity gt 1000 Lk Glass type Euroglass DE Protection film 4 MIL CLEAR Transparency 95 UV protection gt 96 Glass width front sides 4 mm Front cover opening height 245 mm Input cover power consumption 230 V 50 Hz 5 5 1250 W Built in mains outlet 1 pce IP44 Mains outlet maximum power 600 W Fan power lt 400 W Heating element power 800 W Work area 590x510 mm Cabinet inner size 645x575x420 mm Dimensions WxDxH 700x730x995 mm Weight 88 kg Placement desktop SNOILVldIDJdS TLC S Thermostated Laminar Flow Cabinet Laminar airflow
21. BioSan PCR cabinets efficiency in comparison to Class Il BioSafety cabinets Based on the experi ment results PCR cabinets prevent microbial contamination with inactivation efficiency up to 96 but in comparison to Class II BioSafety cabinets do not provide protection against mechanical contamination UV air treatment in BioSan PCR cabinets for 30 min provides DNA amplicon deactivation efficiency e Combined UV treatment Open UV and UV air recirculation provides 10096 efficiency e UV air recirculation provides 99 efficiency e Open UV irradiation provides 100 efficiency Applications Articles Based on classification of BioSafety cabinets from European standard EN 12469 3 and experiment results BioSan PCR Cabinets and Class Il III BioSafety Cabinets were compared on product protection ability in table 4 Further studies will be focused on e Development of high speed monitoring technology of RNA amplicon concentration in the laboratory air and in sterile cabinets e Investigation of Class Il BioSafety cabinets efficiency against DNA amplicon contami nation Based on preliminary experiment re sults DNA amplicon particles which are not stopped by HEPA filters Fig 2 can result in constant contamination of cabinets volume Table 4 Classification of sterile cabinets based on protection against contamination BioSafety cabinets Microorganisms Class Class II A1 A2 B1 B2 Class Ill BioSa
22. actual speed values 3 Relative centrifugal force A brushless rotor provides noiseless performance at the maximal speed and long service life An angular rotor is designed for accommodation of 12 Eppendorf microtubes spin columns The rotor is made of aluminium it is equipped with fixing lid and included in the standard speci fication of the centrifuge Constant airflow around the rotor reduces risk of samples overheating during operation Metal protective inserts inside the casing lid automatic imbalance switch off and locking of a lid provide safe operation Completion of centrifugation is indicated by a sound signal The external power supply unit allows operation of Microspin 12 in cold rooms at ambient temperatures from 4 C to 25 Speed control range 1000 14 500 rpm 100 rpm increment Relative centrifugal force control range 50 12 400 xg Digital time setting 15s 30 min Time setting resolution 1 min 15s after 1 min 1 min Acceleration time up to 14 500 rpm 20 sec Slowdown time not more 10 sec Display LCD 2 line Safety Rotor imbalance diagnostics automatic stop IMBALANCE warning Overall dimensions W x D x H 200 x 240 x 125 mm Weight 3 5 kg Input current power consumption 24V 2 5 A 60W External power Input AC 100 240 V 50 60 Hz supply Output DC 24 V ORDERING INFORMATION Cat number Microspin 12 BS 010213 AA1 Built in rotor MSR 12 12 places for microtubes 1 5 2 ml with p
23. and gives a warning message in case of low solution volume or full waste bottle when Dimensions WxDxH 375x345x180 mm 4 CHW Logger is connected Indication of operation modes 8 line LCD Weight with accessories 11 kg External power supply 12 5 Consumed power not more 60 W 4 CHW Logger Specifications The unit is designed for use in closed laboratory rooms at Max loading per scale cup 2 kg temperatures from 10 to 35 C and relative humidity up to Dimensions 267 252 97 80 at 25 C decreasing linearly 50 relative humidity at Weight 3 kg 35 102 Washers Aspirator DESCRIPTION 3D IW8 Inteliwasher eo ue Product video is available on the website 4 CHW Logger Inteliwasher 3D IW8 series microplate washer is designed for washing various types of standard 96 well microtitre plates microstrips as well as microarrays on FastFRAME rectangular well shape It is suitable for washing wells with different bottom shapes flat U shape and V shape The unit is fully programmable ensuring multi step solution ripening aspiration aspiration combination of aspiration liquid dispensing and soaking as well as soaking cycle during a particular period of time Dispense system of liquid dosage for each channel separately THE UNIT PROVIDES Washing mode Rinsing mode Mixing mode Single point two point circular circle or rectangular path aspiration Possibility of additional solution mixi
24. ating at ambient temperature range 4 C to 40 C Speed control range 20 250 rpm increment 5 rpm max speed depends on the load and vessels shape Digital speed control Max continuous 168 hrs operation time Orbit 20 mm Digital time setting 1 min 96 hrs non stop increment 1 min Maximum load 8 kg Overall dimensions WxDxH 410x410x130 mm Weight 11 7 kg Input current power 12V 3 2 A 40W consumption External power supply Input AC 100 240 V 50 60 Hz Output DC 12V Motion Digital time Speed range Turning angle 20 250 rpm 0 250 sec 1 min 96 hrs 20 250 rpm 0 360 0 250 sec non stop 30 increment increment 1 min 0 5 0 5 lt 1 increment ORDERING INFORMATION Cat number PSU 20i without platform BS 010145 ACI Cat numbers of all platforms can be found on page 15 1 194253 P 21412345 P Devices Rockers Shakers Rotators Vortexes Platforms for PSU 10i and ES 20 Platform up 12 Used on PSU 10i ES 20 Bio PP 4 Used on PSU 10i pp 4 Used on ES 20 P 12 100 Used on PSU 10i ES 20 Description Universal platform with adjustable bars for different types of flasks bottles and beakers with silicone mat Flat platform with silicone mat for Petri dishes culture flasks agglutination cards Metallic flat platform with silicone mat Platform with clamps for flasks 100 150 ml 12 places
25. avoid sample ine orice vessels to be into the bath designed for minimal impact on contamination Accor mpdat di accommodated Working area Refrigerated Heat immersion coolers exchange coil Consist of a cooling coil Designed to be connected to a refrigeration attached to a unit by a flexible pipe supply of cooling Extract heat continuously tap water or with the bath control unit a refrigerated controlling temperature circulator C1G 0 to 40 40 coolan temperature ST5 5L STL5 1 x PS20 1 x QR ST12 12L stainless steel STL12 gabled hinged removable stainless steel S 1 x PS20 2 x VR RS14 ST18 18L stainless steel STL26 gabled hinged removable stainless steel RS22 4 ST26 26L stainless steel STL26 gabled hinged removable stainless steel 2 x PS20 4 x VR RS28 ST38 38L stainless steel STL38 gabled hinged removable stainless steel N 3 x PS20 6 x VR E RS28 or RS38 z b CUM P5 5L PLS 1 x PS20 1 x QR plastic at stainless steel gt Q ys _ _ _ P12 12L PL12 1 x PS20 2 x VR RS14 plastic curved plastic pe lt a E 18 181 PL18 RS22 plastic curved plastic for Rack Systems description see 68 pu ORDERING INFORMATION catalogue number matches the n
26. best calibration curve Save load and export results e Creating visual reports 010 90 New Products and Announcements TS DW Thermo Shaker for Deep Well Plates TS DW Thermo Shaker is designed for shaking and incubating deep well plates A multisystem principle used in the design of the Thermo Shaker allows operating it as 3 independent devices Incubator Plate shaker and Thermo Shaker TS DW provides excellent temperature uniformity across the plate due to patented two sided heating of the block and the lid contour heating of the block and close proximity of heating elements to plate walls There is a number of interchangeable blocks to suit different plates such as Eppendorf 96 1000 ul Sarstedt Megablock 96 2200 ul Porvair 96 2000 ul Axygen 96 2200 ul Also we can manufacture a customized block on request MPS 1 Multi Plate Shaker High mixing speed up to 3 200 rpm Smooth mixing with 3 mm orbit 5 mixing presets Innovative mixing algorithm Pulse Mode Low noise level at maximum speed High stability at maximum speed Universal platform holder for immunoplates deep well plates and platforms for tubes Additional platforms for semi and unskirted PCR plates 200 ul and for tubes from 0 2 to 2 mL Can be used in cold rooms and incubators tem perature range from 4 C to 40 C o mm QU PTS New Products and Announcements ANNOUNCEMENT AA S Bt Smart BioTherm CVP 2
27. closed UV lamp 25 W installed in a special aluminium casing which is located in the upper hood Fan s air flow speed is 14 m hour which processes 1 3 cabinet volumes per minute Distance from closed UV lamp to recirculator s walls is 2 cm at which UV intensity level is 20 mW sec cm Fig 10 UV air recirculators are designed for constant air decontamination during operations Dosage after 15 min 1 800 2 700 mW cm 1 800 5 400 mW cm 1 800 5 400 mW cm Fig 10 UV intensity dependence on distance to UV tube measured by radiometer VLX 254 Vilber Lourmat France UV intensity mW cm sec Distance to UV source cm UV intensity mW cm sec Distance cm e 20 2 e 4 25 e 2 50 Dosage after 30 min 3 600 5 400 mW cm 3 600 9 000 mW cm 3 600 9 000 mW cm Resulting in following UV dosage for cabinet s volume e During 15 min recirculation 380 mW cm e During 30 min recirculation 780 mW cm Cabinet s Surface treatment UV Air recirculator does not provide cabinet surface irradiation For deactivation of microorganisms and ampli cons on the cabinet s surface additional open UV treatment is needed for protection against contamination Conclusions Air sampling methods developed by BioSan has been proven to be compatible with real time PCR detection of product This method enables monitoring of laboratory air and sterile cabinet for presence of target DNA amplicons The research was designed to evaluate
28. diameter specification of 0 3 responds to the most penetrating particle size MPPS Particles that are smaller or larger are trapped with even higher effi ciency 7 Fig 2 Mechanical Filter 1 000 2000 5 000 10000 Yeast Fungi Experimental setup Impaction aerobiocollector airlDEAL 3P bioM rieuxSA France was used to take air samples to test for the presence of colony forming units CFU Each sample was exposed to 500 litres of air Aerobiocol lector was set in the middle ofthe sterile cabinets for test samples and negative control samples and in specific places in the middle of the laboratory room for positive control The negative control was taken in Microflow ABS Cabinet Class Il This was repeated three times the number of colony forming units was counted manually on each plate Reading tables provided in airlDEAL bioM rieuxSA France The most probable number MPN of microorganisms collected per plate was estimated with respect to the number of agglomerates of colonies counted on the plate MPN was calculated from the CFU count using FELLER s law Subsequently results were converted CFU per Applications and Articles Mechanical contamination test Instrument Laser particle counter produced by Met One USA was used to determine mechanical contamination in the sterile cabinets and laboratory air as positive control Method Average amount of particles per litre of air were meas
29. form of the 3D graph see fig 4 Display of the experimental results in the 3D format has one more advantage that the obtained data provides a clear visual tool for the analysis of the complex interrelated processes of cell growth and allows to find the optimal and reproducible param eters obtained for the output of the cell material Growth rate versus time data presented in the graph was obtained during 20 hours long fermen tation process and optical density was measured in a 10 minutes interval The optical density was deter mined in the monolayer of growing cells and growth media formed as a result of a Vortex as described in the legend to fig 3 Volume of the culture medium is taken into account when calculating the length of the optical path of the rotating tube that allows to calculate the optical density in standard values familiar for biotechnologists A 600 nm optical path 10 mm Classical 2D data view of cell growth versus time obtained at the endpoint or during cell growth when cell density was measured at intervals of 1 4 hours do not provide such opportunity Applications Articles Fig 3 Influence of Frequency of Reverse Spinning on the Growth kinetics and Growth Rate AOD A 850nm At vs Time of fermentation hrs Legend of experiment Real Time Cell Growth Logger was used RTS 1 with 850 nm LED Volume of LB media in 50 ml Falcon 15 ml approx Reverse Spin Frequency RSF 1 2 4 8 16 30
30. of cells and biological and chemical liquid components in tubes using eccentric mechanism Vortex has two modes 1 Continuous operation 2 Impulse operation activated by pressing the cap with the tube s bottom Eccentric mixing principle 215412345 P NOlLdId SAC P Speed control range 750 3 000 rpm Mixing module for tubes from 1 5 to 50 ml Maximum mixing volume 30 ml Orbit 4 mm Overall dimensions WxDxH 90x150x80 mm Weight 0 8 kg Input current power consumption 12V 320 mA 3 8 W External power Input AC 100 240 V 50 60 Hz supply Output DC 12V ORDERING INFORMATION Catnumber V 1 plus BS 010203 AAG DANA DESCRIPTION SPECIFICATIONS Devices Rockers Shakers Rotators Vortexes V 32 Multi Vortex Multi Vortex V 32 is intended for intensive stirring of bacterial and yeast cell washing from the culture medium and extraction of metabolites and enzymes from cells and cell cultures It is different from V 1 by the possibility of mixing up to 32 tubes simultaneously Vortex is applicable for e Performing various DNA operations deproteinisation of DNA protein complexes e Purification of low molecular DNA RNA fragments PCR diagnostic Multi Vortex has two operation modes 1 Continuous operation 2 Impulse operation V 32 is supplied with a 32 socket universal platform for Eppendorf type tubes up to 1 5 ml 1 5 0 5 0 2 ml
31. resistivity of 18 2 Mega Ohm cm 0 055 uS cm ultrapure water produced by a Labaqua system exceeds requirements of all relevant standards ISO 3696 Grade 1 ASTM Type l CLSI Type I Purified water is collected in a storage tank An integrated recirculation system ensures consistent quality of water and reduces total organic carbon TOC to very low levels 2ppb Pure water produced by the Labaqua systems complies with the requirements of ISO 3696 Grade 2 water and can be used for labware washing wet chemistry methods flame spec trophotometers etc The dispense rate of ultrapure water is 2 L min Whereas the dispense rate of pure water is 4 L min All Labaqua systems have a controller with a graphic LCD display for water quality indication The LCD display provides all necessary information about system status as well as the remaining pre filter life and deionization DI module performance The smart DI module monitoring system also provides a reduction in running costs A user is instructed to replace the DI module only when the module is near the end of its service life All cartridges and filters are easily accessible and no tools are required to replace them The Labaqua system can be installed on a laboratory bench or mounted on a wall Deionization Input module Recirculation valve Labaqua Labaqua Pure water Shunting i i quality restrictor Ultrapure water Appl Ication HPLC Bio St
32. the amplification Lambda DNA copy number values were esti mated for cabinet volume and surface area results presented in Fig 9 Norm Fluoro Inactivation efficiency was calculated as ratio of DNA amplicons before and after treatment direct and indirect UV treatment for 15 and 30 minutes presented in percents in table 2 Fig 8 Standard curve influence of direct and indirect UV irradiation on lambda phage DNA copy number Concentration copy numbers 1 Samples after Lambda 2 Samples Phage spraying no UV after 30 min UV irradiation positive control inactivation e Concentration Samples standards Table 2 DNA amplicon inactivation efficiency in PCR cabinet UVC T M AR Biosan Latvia Fig 7 Effect of UV irradiation on Ct Cq values raw results Oce Fig 9 Effect of direct and indirect UV irradiation on the amplicon concentration inside PCR cabinet UVC T M AR Biosan Latvia Relative Copy numbers Air Samples Surface Samples After Lambda phage DNA spraying UV Air Recirculator for 15 min Closed UV light irradiation 25 W UV Air Recirculator for 30 min Closed UV light irradiation 25 W Open UV light 25 W irradiation for 15 min Open UV light 25 W irradiation for 30 min The horizontal axis show air or surface samples along with the relative copy number presented on vertical axis Four series represent ina
33. 0 minutes Dosage after 30 min 3 600 5 400 mW cm2 Side walls 10 60 cm 1 800 5 400 mW cm2 3 600 9 000 mW cm2 Front window 10 60 cm 1 800 5 400 mW cm2 3 600 9 000 mW cm2 See the article on page 115 for full information UVC T AR UVC T M AR PCR Oper ations UVC T M AR ULTRAPURE WATER SYSTEMS Labaqua series Labaqua ultrapure systems are multi purpose water purification systems The Labaqua systems produce ultrapure and pure water directly from tap water Labaqua Ultrapure systems are available in the following configurations Labaqua HPLC produces water with very low organic carbon TOC content meeting requirements of liquid chromatography methods Labaqua HPLC water can also be used for some microbiological and molecular biology applications Labaqua Bio system produces water with very low organic and RNase DNase content that is intended for the use in molecular biology including RNase sensitive applications DESCRIPTION NEW PRODUCT Any configuration of a Labaqua ultrapure system produces both ultrapure and pure water Ultrapure Grade 1 water is dispensed idc the point of use filter on the front panel Pure Grade 2 water is dispensed directly from the storage tank M Labaqua ultrapure water can be used for the most demanding applications including but not limited to Inorganic trace analysis Liquid chromatography Cell culture Molecular biology With
34. 12V ORDERING INFORMATION Cat number Multi Bio RS 24 with standard platform PRS 26 Optional platforms BS 010117 AAG PRS 5 12 BS 010117 HK PRS 10 BS 010117 IK PRSC 22 BS 010117 LK PRSC 10 BS 010117 JK PRS 1DP BS 010149 DK Product video is available on the website Programmable Rotator provides 3 rotation types and Pause 1 Cp Vertical rotation Reciprocal rotation T Vibro LN w Q Pause NEW FUNCTION During the stop mode the platform does not perform extra turnover and stops in a horizontal plane Additionally there is a possibility of setting the plane with respect to which platform oscillates vertically or horizontally Multi Bio RS 24 with optional platform PRSC 22 Description and pictures of all platforms can be found on page 22 eo um Product video is available on the website Programmable Rotator provides 3 rotation types and Pause Gp Vertical rotation 0 Q2 Reciprocal rotation Vibro Q Pause OB A a A 9 NEW FUNCTION During the stop mode the platform does not perform extra turnover and stops in a horizontal plane Additionally there is a possibility of setting the plane with respect to which platform oscillates vertically or horizontally Multi RS 60 with standard platform PRS 48 Description and pictures of all platforms can be found on page 22 Devices Rockers Shakers Rotators Vortexes Multi RS 60 Multi Rotator Programm
35. 2 5 8 2 1 5 m 0 5 0 ES 20 Baffled Flask ES 20 Baffled Flask 50 ml 100 ml T Orbit 50 ml RS 6 Baffled Flask RS 6 Baffled Flask i E 565 nm RS 6 Falcon w o 5 6 Falcon w o filter 12 5 ml filter 25 ml 0 RS 6 Falcon with filter 12 5 ml RS 6 Falcon with filter 25 ml 100 ml S 112 Applications and Articles The fig 1 shows a comparative data of the biomass yield obtained for the E coli night culture cultivated in LB medium in Erlenmeyer flasks Shaker Incu bator ES 20 BioSan and in test tube reactors RTS 1 Biomass yield is presented in the optical densities measured at 600 nm and 850 nm wave lengths The results obtained for a tube rotating around its own axis at a speed up to 2 000 revolu tions per minute The experimental data suggests that biomass yield obtained using the Reverse Spin Technology is not lower than biomass amount obtained by tradi tional methods of cultivation and as is the case of cultivation in Erlenmeyer flasks depends on the volume of medium in the flask all other param eters being equal Next we concentrated our efforts on the develop ment of correct cell concentration measurement technology in Real Time As you may know the final concentrations of E coli cells in LB medium significantly exceed OD 1 0 at A 600 nm which requires stopping the process of growing cells sterile sampling and dilution This ma
36. 20 for e Growing cell cultures in flasks and other laboratory glassware e Extracting tissue samples at physiological temperatures e Other sample preparation processes WW ORDERING INFORMATION Cat number ES 20 without platform BS 010111 AAA Description and pictures of all platforms can be found on page 14 Shaker Incubators Personal bioreactors ES 20 60 Orbital Shaker Incubator ES 20 60 with platform P 6 1000 020mm Orbit HEAT UP TIME FOR ES 20 60 ES 20 60 with platform PP 400 Description and pictures of all plat forms can be found on page 15 Orbital Shaker Incubator ES 20 60 for biotechnological and pharmaceutical laboratories is a professional category equipment designed for cultivation of microorganisms and eukaryotic cells including animal plant and insect cells It is also possible to cultivate thermofilic bacteria in ES 20 60 shaker incubator Shaker is equipped with a direct drive mechanism for plat form motion It provides reliable and stable operation for the long term experiments needed for cell growth Shaker Incubator ES 20 60 provides smooth or intensive mixing in flasks installed on the platform Built in noiseless thermoresistant brushless fan provides precise temperature distribution inside the chamber adjustable for up to 80 C The inner chamber is made of stainless steel State of the art motor newest thermal insulation materials soft start of the platform motion and temper
37. 70 mm LF 1 Weight 28 kg Overall dimensions W x D x H 300 450 705 mm ORDERING INFORMATION Cat number x T 4 BS 040101 BK T 4L BS 040107 BK T 4L P BS 050101 CK LF 1 BS 050101 BK General Information SAFETY All Biosan laboratory equipment meets the require ments of International Standard IEC 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use and applicable specific parts e g IEC 61010 2 010 requirements for laboratory equip ment for the thermostating equipment IEC 61010 2 020 for centrifugation equipment IEC 61010 2 051 for magnetic stirrers CE MARK All Biosan laboratory equipment bears a CE mark to indicate that it meets the requirements of all applicable European Directives Compliance with the Low Voltage Directive is demon strated by meeting EN 61010 as indicating in para graph on safety and the EMC Directive by meeting EN61326 1 EMC requirements for electrical equipment for measurement control and laboratory use ELECTRICAL SUPPLIES All standard Biosan laboratory equipment is available for voltages within the range 220 240 V 50 or 60 Hz Most of the equipment is also available for voltages 100 120 V 50 or 60 Hz ENVIRONMENTAL CONDITIONS Biosan laboratory equipment is designed for indoor use in laboratory conditions with room temperature between 4 C and 40 C and 8096 relative humidity up to 31 C unless stat
38. A filtered sterilie airflow the cabinet has two more addi tional options 1 Termostated laminar flow of sterilized air 2 Recirculation systems with ultraviolet light irra diation to inactivate viral particles DNA and RNA amplicons in the recirculated air NOUN D P N DESCRIPTION 1 LAMINAR AIRFLOW Highly efficient filtration is provided by two pleated HEPA filters that meet the requirements of protection class H14 Filters installed in a cabinet are effective not less than 99 995 if trapping particles of 0 3 micron In a standard mode the inflow air enters through the perfo rated front grille without penetrating into the working area The recirculated air in the working area enters the grille in the perimeter of the working area All the air then flows upwards where 7096 of the air is recirculated through the main HEPA filter and is directed to the working area as a laminar flow while 3096 of the air is directed out of the cabinet through the second same temperature The preheated airflow protects cell HEPA filter The intensity of the fan is adjusted lines from a possible heat shock and prevents conden automatically keeping the speed of the laminar sation of liquid on the walls of the cell culture vessels flow in the cabinet within 0 25 0 50 m s in through the uniform temperature distribution within accordance with the requirements of BSC Class the laminar flow cabinet 2 and EN 12469 Working area
39. AAA BS 040109 AAA BS 040109 A05 BS 040104 AAA BS 040104 A06 BS 040107 AAA BS 040101 BK BS 040107 BK BS 050101 CK BS 050101 BK Model Wall materials Working surface material l ua Z lt U A Uu Open UV lamp UV radiation level Radiation type Digital time setting of direct UV exposure UV recirculator Daylight lamp for work ing area illumination Thickness of side panels Thickness of upper front panel Thickness of the front protective screen Optical transmission UV protection Working area dimensions Safety features Power outlets inside the unit Nominal operating voltage Power consumption 23072120 V Overall dimensions WxDxH Weight net gross Optional table see lab furniture on page 105 UVC T AR UVC T M AR UVT B AR UVT S AR compact compact compact double size Rear stainless Rear stainless steel Plexiglas steel Sides steel with Rear stainless steel Polymethyl Sides and chemical resistant Sides and front glass methacrylate front glass powder coating Front EUROGLASS ALTUGLAS EX EUROGLASS glass EUROGLASS Germany Germany Germany Steel with chemicals Stainless steel resistant powder coating 2 x 30W built in 1x 25W built in bactericidal bactericidal Philips TUV25WG13 UV C lamps Philips TUV30WG13 UV C 15 mW cm sec UV A 253 7 nm ozone free 1 min 24 hrs non stop increment 1 min 1 x 30 W
40. Baths Heating cooling Circulators SUB Aqua Pro Digital Unstirred Water Bath Directs condensation away from immersed vessels avoids contamination reduces evaporation and saves energy S SAP2 SAP2S SAP5 SAP12 SAP18 SAP26 SAP34 SAPD O 2n 2n 5L Tm 18L 26L 36L SLE j Replacement polycarbonate transparent lids L AQL5 U AQL2 AQLS AQLS AQL12 AQL26 AQL26 AQL12 LL UU LLI a Un LU6 LU6 LU14 LU28 LU28 LU36 LU6 amp LU14 LF6 LF14 LF28 LF28 LF36 LF6 LF14 2ringsets Aringsets 6 ring sets 6 ring sets 8ring sets With ring sets of variable hole diameter to accommodate tall vessels whilst reducing evaporation 1x PS20 12520 1 x PS20 1 x PS20 2 x F520 2 x PS20 3 x P520 2 x PS20 Useful alternative to a lid minimises evaporation and heat loss whilst allowing easy access to vessels in the bath particularly useful for tall vessels RS14H RS18H RS28H RS36H RS14H h 40 or 78 h 40 or 135 h 45 or 135 h 45 or 135 h 40 or 78 shelf covers shelf covers shelf covers shelf covers shelf covers half half area of half area of half area of half area of area of SAPD SAP12 SAP18 SAP26 SAP34 1xJ2 2x2 4 x J2 4 x J2 6 x J2 1 2xJ2 Choice of 8 variants to accommodate different tube diameters and microtubes see below AQBT5 amp AQBT2 AQBT5 AQBT5 AQBT12 AQBT26 AQBT26 SBT36 12 Required if flat bottomed flasks are to be placed directly on the
41. DS Cytochemistry for in situ reactions TS DW Thermo Shaker is designed for shaking and incu bating deep well plates A multisystem principle used in the design of the Thermo Shaker allows operating it as 3 independent devices Incu bator Plate shaker and Thermo Shaker TS DW provides excellent temperature uniformity across the plate due to patented two sided heating of the block and the lid contour heating of the block and close proxim ity of heating elements to plate walls There is a number of interchangeable blocks to suit differ ent plates such as Eppendorf 96 1000 ul Sarstedt Mega block 96 2200 ul Porvair 96 2000 ul Axygen 96 2200 ul Also we can manufacture a customized block on request DEEP WELL PLATE THERMO SHAKER PROVIDES Soft or intensive sample shaking e Rotation speed regulation stabilization and indication Even rotation amplitude throughout the Thermo Shaker platform e Exceptional temperature uniformity across the plate Required operation time setting and indication e Automatic stopping of the platform movement after expiration of the set time Setting and indication of the required temperature on the platform A variety of changeable blocks that can accommodate most popular deepwell plates Temperature setting range 25 C 100 C Temperature control range 5 C above ambient 100 C Temperature setting resolution Temperature uniformity 37 0 1
42. ER BATHS ORBITAL LINEAR SHAKING BATHS UNSTIRRED WATER BATHS HEATING COOLING CIRCULATORS Stirred water bath WB 4MS Orbital Linear Shaking Bath OLS26 LSB Aqua Pro Unstirred Water Baths Sub Aqua Pro Heating Cooling Circulators Grant Optima series Optima R series SHAKER INCUBATORS PERSONAL BIOREACTORS MAGNETIC STIRRERS OVERHEAD STIRRER Magnetic Stirrers MS 3000 MMS 3000 MSH 300 Intelli Stirrer MSH 300i Overhead Stirrer MM 1000 BIOSAFETY EQUIPMENT BIOSAFETY AIR UVR M and UVR Mi UV Cleaner Recirculators DNA RNA UV Cleaner Boxes UVC T AR UVC T M AR UVT B AR UVT S AR TLC S BIOSAFETY WATER Labaqua Series PHOTOMETERS Densitometers DEN 1 DEN 1B Microplate Photometer HiPo MPP 96 WASHERS ASPIRATOR Washers IW 8 3D IW8 Aspirator FTA 1 LABORATORY FURNITURE T 4 T 4L T 4L P LF 1 GENERAL INFORMATION General information about Biosan policy APPLICATIONS AND ARTICLES The World of Biotechnomica Reverse Spin Technology Innovative Principle of Microbial Cultivation Development and evaluation of DNA amplicon quantification UVR M and UVR Mi UV Air Recirculators Test Report How to Choose a Proper Shaker Rocker Vortex Product Line Examples 108 110 115 123 127 128 Table of content Nn E 2 4 New Products Announcements ANM RTS 1 RTS 1C Personal bioreactors RTS 1 and RTS 1C are personal bioreactor
43. ING INFORMATION HiPo MPP 96 Optional accessories Calibration Plate Cat number BS 050108 A02 BS 050108 AK ANNOUN VIE IN e INFORMATION ABOUT THE AVAILABILITY OF THE DEVICE WILL BE PUBLISHED ON OUR WEBSITE WWW BIOSAN LV p iif EEE creta i DOG DDD d ONO NE NS ERN EN ZENA Accuracy at 450 492 nm 0 000 2 000 OD lt 0 5 96 0 010 OD typical 2 000 3 000 OD lt 196 0 010 OD typical Precision Reproducibility at 450 492 nm 0 000 2 000 OD lt 0 5 96 0 005 OD 2 000 3 000 OD lt 1 0 96 0 005 OD Linearity 0 000 2 000 OD 1 0 96 2 000 3 000 OD 15 CATALOGUE 2015 2016 WASHERS ASPIRATOR 100 Washers Aspirator DESCRIPTION ORDERING INFORMATION Cat number IW 8 Intelispeed Washer m 4 CHW Logger Intelispeed Washer IW 8 is designed for washing of standard flat bottom 96 well plates and microstrips The unit is fully programmable ensuring multi step solution ripening aspiration aspiration combination of aspiration liquid dispensing and soaking as well as soaking cycle during a particular period of time The unit has 100 user defined programs Standard version is supplied with 8 channel washing head for dispensing aspiration 3 bottles for washing and rinsing solutions a waste bottle and bottle with filter Optional 4 channel washing solution weight logger 4 CHW Logger is available The unit is designed fo
44. K Results after 1 500 rpm spin for 9 minutes using Grifols Lateral Gel Cards CATALOGUE 2015 2016 THERMOSTATED EQUIPMENT THERMOSTATS DRY BLOCK HEATING COOLING SYSTEMS SPECIFICATIONS B DESCRIPTION 1 Thermostated equipment Thermostats Dry Block Heating Cooling systems Bio TDB 100 Dry Block Thermostat Bio TDB 100 is a compact easy to use dry block ther mostat designed for long incubation processes at various temperatures The universal aluminium block can accommodate 3 most popular microtest tubes types Simultaneous indication of set and actual temperature and time Temperature setting range 25 C 100 Temperature 5 C above ambient 100 C control range Temperature setting resolution 0 1 C Temperature stability 0 1 C Temperature uniformity 37 C OTC Digital time setting 1 96 hrs non stop increment 1 min Display LCD 2x 16 signs Block diameter depth O 130 mm 45 mm Block capacity 24x2 1 5 ml 15x0 5 ml 10x0 2 ml microtubes Overall dimensions W x D x H 210x230x115 mm Weight 2 8 kg Nominal operating 230 50 60 Hz voltage or 120 V 50 60 Hz Power consumption 230 V 200 W 870 mA ORDERING INFORMATION Cat number Bio TDB 100 with built in block BS 010412 AAA i HEAT UP TIMES FOR BIO TDB 100 from 25 from 25 to 100 C to 37 C Be Bech rat LOI 7 7 Thermostated equipment Thermos
45. Linear shaking speed 20 to 200 rpm depending on load Shaking speed display 1 rpm resolution Linear shaking stroke 20 mm length Shaking tray area 240 x 235 mm 420 x 235 mm Timer 1 to 999 mins Heater power 120 230V 0 8 0 8 kW 1 05 1 4 kW Drain tap yes Safet over temperature protection low liquid cut out y Supply voltage 110 120V or 220 230V ORDERING INFORMATION Cat number LSB12 Linear shaking bath 12 L LSB12 LSB18 Linear shaking bath 18 L LSB18 Description and pictures of all available accessories can be found on page 65 Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heatina cooling Circulators Accessories for Shaking Baths LSB 12 LSB 18 amp OLS 26 ORDERING INFORMATION Accessories LSB and OLS Aqua Pro Product description OLS26 LSB12 LSB18 Universal tray with adjustable springs Highly TU26 TU12 TU18 versatile for a variety of vessel types Flask plate tray with threaded holes to accept flask clamps or holder for deep well plates 22ml See TF26 TF12 TF18 options below Test tube tray compatible with SR racks or can be 1526 1512 7518 used alone to accommodate bags and miscellaneous holds up to 5 SR inn s n vessels See rack options below racks tacks racks Base tray perforated stainless steel allows bath to be SBT26 SB
46. N SPECIFICATIONS Thermostated equipment Thermostats Block Heating Cooling systems CH 100 Heating Cooling Dry Block CH 100 is the result of combining two popular Biosan instruments 1 Heating Dry block and 2 Cooling Dry block thermostat The combined construction of aluminium block and Peltier element module cooled with the forced ventilation radiator provides fast changing of the cooling and heating modes CH 100 is a very effective instrument of sample prepara tion during enzyme reactions hybridization reactions DNA analysis Microprocessor controlled time and temperature Simulta neous indication of set and actual temperature and time Temperature setting range 10 C 100 C Temperature control range 30 C below ambient 100 C Temperature setting resolution Temperature stability 0 1 C Temperature uniformity 37 C EOC Digital time setting 1 min 96 hrs non stop increment 1 min Display LCD 2x 16 signs Overall dimensions WxDxH 240 x 260 x 165 mm Weight 3 2 kg Input current power consumption 12V 4 4 A 55W Input AC 100 240 V 50 60 Hz Output DC 12V External power supply BLOCKS CAPACITY Block CH 1 20 x 0 5 ml 12x lt 1 5 ml microtubes Block CH 2 20x 1 5 ml microtubes Block CH 3 20 x 2 ml microtubes the blocks are built in ORDERING INFORMATION Cat number CH 100 with block CH 1 CH 100 with block CH 2 CH 100 with block CH 3 B
47. PST 60HL 4 and PST 100HL Thermo Shakers Plate Thermo Shakers are designed for shaking and thermo stating 1 4 standard 96 well microplates A multisystem principle used in design of the Thermo Shaker allows operating it as 3 independent devices e Incubator e Microplate shaker o Thermo Shaker A distinctive feature of Biosan Plate Thermo Shakers is the patented by the company Two Side Microplates Heating which allows to achieve full correspondence of the set and actual temperature in the microplate wells Standard versions of thermo shakers provide heating up to 60 C which is sufficient for carrying out ELISA tests Thermo shaker PST 100HL with the ability to stabilize the temperature up 100 Cis specially designed for the hybridi zation reactions PLATE SHAKER THERMOSTAT PROVIDES e Soft or intensive sample shaking Rotation speed regulation stabilization and indication e Even rotation amplitude throughout the Thermo Shaker platform Required operation time setting and indication e Automatic stopping of the platform movement after expira tion of the set time Setting and indication of the required temperature on the platform e Automatic fault diagnostics temperature sensor platform heating lid heating etc APPLICATION FIELDS Cytochemistry for in situ reactions Immunochemistry for ELISA reactions Biochemistry for enzyme and protein analysis Molecular biology for micro
48. QR LE 1 5 ml 35 QR LE A Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators Combined Orbital Linear Shaking Bath OLS26 Patented combined orbital and linear shaking mechanism EN of the OLS26 allows optimisation of aeration and shear forces mixing for reproducible results UO Un e Precision digital temperature control C e 0 C to 99 C operating range 22 Stability 0 1 E e Easy changeover from linear to orbital shaking O e Adjustable shaking speed and stroke length 2 e Polycarbonate lid included as standard e Drain tap for convenient emptying e year warranty e For flexibility tray sold seperately Tank size 26L Minimum working depth 70mm WGC Temperature ambient 5 to 99 C 0 to 99 C aS range with accessory cooling a Uniformity DIN 12876 3 70 C 0 1 C Stability DIN 12876 3 70 C EO TC a Display 2 x LED individual displays and controls for J gt temperature and shaking speed 1 Orbital and Linear shaking speed 20 to 200 rpm O depending on load 22 Orbital shaking radius 9 mm d Shaking speed display resolution 1 rpm Linear shaking stroke length 18 28 and 36 mm Shaking tray area 380 x 235 mm Timer 1 to 999 mins Heater power 120V 230V 1 05 1 4 kW Drain tap yes Safety over temperature protection Equipment presented on pages 63 74 is le by Grant Instru Cambridge Ltd Biosan is a sole distributor
49. S 010410 BAI BS 010410 CAI BS 010410 UAI Ice on block CH 2 CH npa E HEAT UP AND COOL DOWN TIMES FOR CH 100 from 25 C to 37 C from 25 C to 100 C from 100 C to 10 C CH 100 Thermostated equipment Thermostats Block Heating Cooling systems HEAT UP AND COOL DOWN TIMES FOR CH3 150 from 25 C to 100 C from 25 C to 3 C INTERCHANGEABLE THERMOBLOCKS B2 50 mm 2 sockets depth 58 mm B10 16 O16 mm x 10 sockets depth 56 mm 86 25 025 mm x6 sockets depth 40 mm B23 1 5 23 sockets for 1 5 ml microtest tubes depth 35 mm B10 13 13 mmx 10 sockets flat bottom depth 30 mm B529 mm x5 sockets flat bottom depth 40 mm B18 12 18 sockets for 12x75 mm round bottom tubes Different block types can be provided on request ORDERING INFORMATION Cat number CH 3 150 without blocks Optional blocks BS 010418 AAA B2 50 BS 010418 AK B10 16 BS 010418 BK B6 25 BS 010418 CK B23 1 5 BS 010418 DK 52 50 5625 CH3 150 Combitherm 2 Combitherm 2 CH3 150 is specially designed to thermo stabilise materials at temperatures from 3 C to 150 C according to methods of analysis To obtain useful function ality and decrease foot print of instruments Combitherm 2 thermoblocks combined in a common electronic circuit board as well as inside a common external body The left part of the front keyboard is responsible for setting parameters for co
50. Stirrer Multi Mixer MM 1000 on a stand Overhead Stirrer Multi Mixer MM 1000 is designed for stirring liquids up to 20 litres Quiet and reliable mixer that can provide stable continuous mixing up to 7 daynights It can realize three types of motion Rotational Reciprocal Vibration MM 1000 performs separate mono 1 2 3 consecu tive binary cycles c 1 2 x c 1 3 x c and 2 3 x c and complex tri cycles 1 2 3 Speed angle and time of stirrer rotation is under micropro cessor control Multi mixer can be used for stirring solutions up to the la medium viscosity range from 1 000 to 10 000 mPa s It 3t cz is an ideal instrument for biotechnology organic synthesis 2 analytical laboratories Multi mixing The innovative combination of three motion types provides high level of homogeneity due to consecutive combination of laminar and turbulent flows that cause substances to dissolve faster Electrically safe and energy efficient powered by 12 V external power supply SPECIFICATIONS OF MOVEMENT TYPES Q Rotation Speed regulation range 40 1 000 rpm Time 0 250 sec Reciprocal motion Turning angle 0 360 increment 30 Time 0 250 sec O Rodana base Vibro motion Turning angle 0 5 increment 1 Timer 0 5 sec Maximal stirring volume water 20 MM 1000 Digital time setting 1 min E Overhead Stirrer Multi Mixer Overall dimensions WxDxH 140 x 135x250 mm TE
51. T Viro Te Weight 2 4 kg LL Input current power consumption 12 V 700 mA 8 4 W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V CATALOGUE NUMBER MM 1000 without stirrers Magnetic Stirrers Overhead Stirrer Accessories for MM 1000 Cat number BS 010306 AAH Optional accessories Type Dimensions Cat number O MP 1 Paddle stirrer 378 x 70 x 70 x8 mm BS 010306 AK 2 Propeller stirrer 2 folding blades 326 x 55 x 8 mm BS 010306 BK Q MP 3 Propeller stirrer 3 folding blades 325 x 50 x 8 mm BS 010306 CK 0 MA 1 Anchor stirrer 332 x 90 x 8 mm BS 010306 DK Q MC 1 Centrifugal stirrer 358x 60 110 x 8mm BS 010306 EK Double clamp For device mounting VELA00001301 Rod and base page 80 For device mounting 40 x 30x 87cm VELAO0001300 v Q Double clamp vr Q CATALOGUE 2015 2016 BIOSAFETY EQUIPMENT BIOSAFETY AIR BIOSAFETY WATER DESCRIPTION UVR M and UVR Mi UV Cleaner Recirculators UVR M and UVR Mi are applicable for UV air inactivation in research laboratory rooms waiting rooms in outpatients departments surgeries and veterinary stations The devices provide complete protection from direct ultraviolet radiation ADVANTAGES OF UVR MI e UVR Mi is the more powerful model of UV air flow cleaner with two UV lamps e Full user protection from direct UV radiation Two operation modes
52. T12 SBT26 used as an unstirred bath Cooling coil source of constant cooling to enable bath to be operated at or below ambient down CC26 to 0oC LS200 lid with access hole for cooling coil recommended Heat exchange coil attach to a cold water supply or refrigerated circulator Can be used down to 20C CW26 above the temperature of the coolant LS200 lid with access hole for cooling coil recommended Stainless steel lid gabled LS200 LU14 LU28 Replacement polycarbonate lid clear gabled AQL26 AQL12 AQL26 catalogue number matches the name of the product EE Thermostatic equipment Water Baths Orbital linear shaking Baths 66 Unstirred Water Baths Heating cooling Circulators Unstirred Water Bath 12L SUB Aqua Pro advanced water bath range with a choice of 8 models Supplied with base tray lid and drain on larger bath m 2L shallow Dual 5L amp 12L 18L _ 26 12L JB Academy basic range with a choice of 3 models Supplied with base tray JB Nova general purpose water bath range with a choice of 4 models Supplied with base tray lid and drain on larger bath SBB Aqua Plus boilingbath range basic range with a choice of 3 models Supplied with base tray The reliability quality and consistent performance of Grant products have made Grant a leading manufacturer of water baths for decades e Anew era for Grant water baths now all mod
53. Thermophillus sp and anaerobic bacteria Lactobacillus acido philus have been optimized Scientific and applied valuable aspects of single used tube RS Reactors and their potential niche in different biotechnological fields are discussed The principles of mixing solutions are among one of the key fields in Bioengineering science Area of mixing is not limited to bioreactors mixing is also essential in the study of biochemical and molecular biological processes Non invasive mixing technology includes a different way of tubes agitation as shown in the table 1 Absence of agitators inside the reactor gives opportunities to use Reverse Spinner as a rotating spectrophotometer spectra cells which measures optical density in the reactor in Real Time Software makes it possible to set optimal param eters of fermentation registers and logs all parameters mixing intensity temperature of the process optical density and cell concentration speed of the growth etc Data Logging and Analysis RTS 1 4 Instruments in 1 Measuring Mixing Thermostating Table 1 Comparison of Non invasive mixing methods Icon Motion Instrument Max V Overhead Rotation Reciprocal Hand type Reverse Spinning Initiation of the Vortex Type Mixing VTM and depth of the Vortex cave depend on 1 angular speed of RS Reactor 2 time from initiating rotation of RS Reactor 3 growth media viscosity 4 temperature These param
54. able Rotator Multi RS 60 performs several motion types in one module Microprocessor control allows performing not only Vertical rotation of the platform but also Reciprocal rotation as well as Vibration These three motion types can be performed separately pairwise and in cycles periodically repeating the sequence of three motion types Multi rotation option of Biosan instruments substan tially expands possibilities and enhances efficiency of sample preparation for the examined materials and allows adjusting the mixing procedure according to the individual tasks Programmable Rotator can be used for variety of applications in modern life science laboratories for hybridization reactions cell growing soft extraction and homogenisation of biological components in solutions as well as for reactions of binding and washing of magnetic particles Vertical rotation Speed control range 1 100 rpm increment 1 rpm Vertical rotation movement overhead 360 Time setting range 0 250 sec Reciprocal rotation Speed control range 1 100 rpm increment 1 rpm Tilt angle range 1 90 increment 1 Time setting range 0 250 sec O vibro rotation Tilt angle range 0 5 increment 1 Pause Vibro time setting range 0 5 sec General Specifications Digital time setting 1 min 24 hrs non stop increment 1 min Maximum load 0 8 kg Overall dimensions WxDxH 430x 230x 230 mm Weight 3 8 kg Input current power 24V 750mA 18W consumptio
55. ame of the product Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators Optima R series Refrigerated Thermostatic Baths and Circulators TXF200 R5 TXF200 R4 TX150 R3 120 82 T100 Rh1 Cost effective and efficient multi purpose systems for The Grant Optima LTC2 Kit includes TC120 heating low temperature applications circulator and R2 tank refrigeration unit Powerful precision cooling whether used in open loop or closed loop format Combining legendary quality reliability and design for everyday usage useful features straightforward maintenance compact design Robust durable construction for longevity reliability and long term low cost of ownership e A complete range 18 models to cover basic through to sophisticated needs All refrigeration products come with market leading 3 year warranties gt 1 14253 P Grant low temperature circulators provide a source of precision cooling for many sensitive analytical proce dures including spectrophotometry viscometry refrac tometry and electrophoresis They are suitable for use in both open and closed loop circulation i e remote vessel open or closed TEMP Alternatively Grant RC series of recirculating chillers We recommend the following liquids for use with closed circulators can be used These are generally refrigerated thermostatic baths and circulat
56. array analysis ORDERING INFORMATION Cat number PST 60HL BS 010119 AAI PST 60HL 4 BS 010128 AAI PST 100HL BS 010142 AAI um Product video is available on the website PST 60HL PST 60HL 4 rio Thermo Shakers Shakers Incubators Real Time Cell Growth Logger PST 60HL PST 60HL 4 and PST 100HL Thermo Shakers Specifications PST 60HL PST 60HL 4 PST 100HL e Temperature setting range 25 C 60 C 25 C 100 C o m Temperature control range 5 C above ambient 60 C vene a n C TI Temperature setting resolution 0 1 C Temperature stability EOC gt Temperature uniformity 37 0 25 0 25 0 2 Double heating contour of the platform and heated lid 7 Orbit 2mm Speed regulation range 250 1 200 rpm increment 10 rpm Digital time setting 1 min 96 hrs non stop increment 1 min Display LCD 16x 2 signs Max height of microtest plate 18mm Number of microtest plates 2 4 2 Weight 6 1 kg 8 8 kg 5 9kg Platform dimensions W x D 250x150 mm 210x290 mm 250x150 mm Overall dimensions W x Dx H 270x260x125 mm 380x390x140 mm 270x260x125 mm Input current power consumption 12V DC 3 3 A 40W lay eet pul 12V 5A 60W External power supply Input AC 100 240 V 50 60 Hz Output DC 12 V HEAT UP TIME PST 60HL AND PST 60HL 4 HEAT UP TIME PST 100HL from 25 C to 37 C toc I to 100 PST 60HL from 37 C to 60 C PST 60HL 4 DESCRIPTION SPECIFICATIONS T
57. ature PID control decrease the energy consump tion and make the Shaker Incubator highly energy efficient despite its relatively large size 50 250 rpm increment 10 rpm 25 C 80 C 10 C above ambient 80 C Speed control range Temperature setting range Temperature control range Temperature setting resolution 0 1 C Temperature stability 05 Maximum load 8 kg Orbit 20 mm 1 min 96 hrs non stop 1 min increment Digital time setting Display LCD 2x 16 signs Maximum continuous operation time 30 days Overall dimensions W x D xH 590x525x510 mm Dimensions of the inner chamber 460 x 350 x 400 mm Weight 41 1 kg 230V 50 60 Hz or 120 V 50 60 Hz Power consumption 230 120V 450 W 2 A 450 W 4 5 A Nominal operating voltage ORDERING INFORMATION Cat number ES 20 60 without platform BS 010135 AAA Optional platforms PP 400 with silicone mat P 30 100 BS 010135 BK P 16 250 BS 010135 CK P 9 500 BS 010135 AK BS 010135 FK P 6 1000 BS 010135 DK UP 168 without clamps BS 010135 JK Clamp FC 100 BS 010126 HK Clamp FC 250 BS 010126 JK Clamp FC 500 BS 010126 LK Clamp FC 1000 BS 010126 IK NOlILdld5s3CG D 5 1 21412345 J 38 Shaker Incubators Personal bioreactors RTS 1 and RTS 1C Personal bioreactors SPECIFICATIONS Measurement range 0 10 OD at 10 20ml volume 0 24 0 8 OD at 20 30ml volume 0 19 OD Measurement Wavelength A 850 nm Measurement Precision
58. bacteria concentration in a rotating reactor As a result proposed RTS 1 technology excludes sampling and dilution procedure that is especially dangerous for harmful bacteria pathogens or microorganisms living in extreme conditions like Thermophilus Results will be published in next issue of BioSan Analytica Journal T Applications Articles Development and evaluation of DNA amplicon quantification Case study UV Cabinet with UV Air Recirculator UVC T M AR and Class II Biological Safety Cabinets Authors Biotechnomica Marina Tarvida Julija Isakova s E otech nom Vasily Bankovsky ee Dp Bu B gn CED UVC T M AR UV Cabinet for PCR operations ee eo 000 000 00 600 Biosan Arturs Kigitovics Vadim Gimelfarb Introduction Personal and product safety during clinical and laboratory studies have stimulated the develop ment of sterile cabinets and special laboratory safety techniques to protect the environment operator and product Monitoring DNA RNA amplicon concentration in laboratory air in sterile cabinets has become topical as PCR and isothermal amplification technologies have developed along with wide spread mass analyses Development of methods for repeatable DNA RNA amplicon detection in air samples is now a reality Recent research Behaviour of aerosol particles in fibrous structures Igor Agranovsky s PhD thesis 2008 Novosibirsk Russia describes the
59. base of the bath and to promote thermal convection in the bath lid or soheres recommended for use above 60 C Unstirred Bath Racks J2 10 10mm 84 J2 25 25 mm 18 J2 13 13mm 55 J2 30 30mm 12 TESTS 16 mm 36 0 5 ml 105 J2 19 19 mm 32 J2 LE 1 5 ml 65 S ORDERING INFORMATION catalogue number matches the name of the device Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators Optima Series Stirred Thermostatic Baths and Heating Circulators TX150 ST26 TXF200 ST38 T100 ST5 TX150 ST18 TC120 5T12 A cost effective range of multi purpose systems combining Grant s legendary quality and reliability Precise temperature control for a wide range of laboratory applications e Accurate and safe temperature control for samples and users e Intuitive programming and thoughtful design features makes working with Grant stirred baths and circulators easy e Robust durable construction for longevity reliability and long term low cost of ownership e A complete range 32 models to cover basic through to sophisticated needs each model represents excellent value for money Applications Grant stirred baths and circulators provide a source of precision heating and cooling for many routine and sensitive analytical T100 P12 with lid procedures including sample incubation calibration and quality control testing Al
60. biological laboratory research WB 4MS provides increased temperature stabilization up to 0 1 C due to built in magnetic stirrer regulated speed 250 1 000 rpm Easy set up high temperature maintenance accuracy compact size and attractive modern design make this water bath widely used mu enm hath Ug aus WB 4MS with base BP 1 on the bottom Tank capacity 4 litres Temperature setting range 25 C 100 Temperature control range 5 C above ambient 100 C Temperature setting resolution 0 1 C Temperature stability TOC Temperature uniformity 37 C 0 1 Stirring speed control range 250 1 000 rpm Digital time setting 1 min 96 hrs non stop increment 1 min Display LCD 2x 16 signs Base BP 1 Digital setting of temperature time and mixing speed Plastic lid with stainless steel interior included z ue Quiet operation Working volume 235x135x110 mm Overall dimensions WxDxH 340x 270 x 250 mm Weight 3 4 kg Nominal operating voltage 230 V 50 60 Hz or 120 V 50 60 Hz Power consumption 230 V 50 Hz 600 W 2 6 A 120 V 60 Hz 670 W 5 6 A 100 V 50 60 Hz 600 W 6 0 A Rack ar 30 ORDERING INFORMATION Cat number WB AMS with base BP 1 and lid BS 010406 AAA Optional racks QR racks Tube size Capacity Cat number R13 10 13 mm 30 QR 13 o 16 19 mm 16 QR 19 o 024 mm 10 QR 24 o 30 mm 5 QR 30 E 7 O OR SE 0 5 ml 44 QR SE j O
61. bodies and immune response 3 Terra Biochemica metabolomics diag nostics of metabolism products and ferment activity 4 Terra Cellomica diagnostics at the level of cel lular morphogenesis cellular polymorphism The distance from the planet orbitals to Terra Innovatica corresponds to the time of disease detection at each level from one week as in the case of DNA analysis to several years when the changes can be traced at the cellular level By virtue of genetic nature of the majority of diseases of human beings animals and plants further affecting the immune response defence reaction and changes in biochemical status and finally cellular morphogenesis as well we believe that simultaneous multilevel diagnostics is reasonable Since polymorphism at the level of genes leads to the manifestation of polymorphism at all higher levels it results in the ambiguity if not more of any decision made on the basis of the obtained data The definition comprising the polymorphism of norm and abnormality disease is not yet available hence the multidiagnostic technology though expensive is the only solution as of today Although the classic determinism in diagnostics has finally yielded its position to the stochastic one there are still no instrumental solutions allowing to channel our new knowledge into informed and unambiguous decisions This is the real situation these arethe temporary sacrifice of progress Bi
62. carbon TOC content meeting require ments of liquid chromatography methods Labaqua HPLC water can also be used for some microbiological and molecular biology applications Labaqua Bio system produces water with very low organic and RNase DNase content that is in tended for the use in molecular biology including RNase sensitive applications MIXING DEVICES ROCKERS SHAKERS ROTATORS VORTEXES SPECIFICATIONS DESCRIPTION Devices Rockers Shakers Rotators Vortexes MR 1 Mini Rocker Shaker Mini Rocker Shaker MR 1 provides regulated gentle rocking motion of the platform and is ideal for mini gel destaining after electrophoresis conducting Northern Southern and Western blot analysis Shaker is a compact noiseless device designed for personal use The use of direct drive and brushless motor allows continuous mixing up to 7 days and ensures reliable trouble free operation for more than 2 years Mt Non slip temperature resistant silicone mat located on the aA rocker s platform provides stable position for vessels during gt shaking Optional dimpled PDM mat fixes tubes of different Ow AC Ms sizes biotan inl Rocker Shaker UID Shaker can be used in cold rooms or incubators operating at ambient temperature range 4 C to 40 C DA Mixing frequency range 5 30 oscill min Fixed tilt angle 7 Rocking uni rotation Max continuous
63. ccus sphaeroides 15 4 Mycobacterium tuberculosis 1 0 Neisseria catarrhalis 8 5 Phytomonas tumefaciens 8 5 Proteus vulgaris 6 6 Pseudomonas aeruginosa 10 5 Pseudomonas fluorescens 6 6 S typhimusium 15 2 Salmonella 10 0 Sarcina lutea 26 4 Sarratia marcescens 6 1 Dysentery bacilli 4 2 Shigella paradysenteriae 3 2 Spirillum rubrum 6 1 Staphylococcus albus 57 Staphylococcus aureus 6 6 Streptococcus hemolyticus 5 5 Streptococcus lactis 8 8 Streptococcus viridans 3 8 Results Applications and Articles Other microorganisms Energy mW seconds per cm List continued from the previous page MOLD SPORES UV Intensity measurements of Philips 25W bactericidal UV lamp UV intensity depends on the distance from the UV source The graph below shows that UV intensity drops dramatically as the distance increases Dependence of UV intensity over distance to the UV source one lamp 25 W UV intensity mW cm Distance to UV source cm Penicillium roqueforti 26 4 Penicillium expansum 22 0 Penicillium digitatum 88 0 Aspergillus glaucus 88 0 Aspergillus flavus 99 0 Aspergillus niger 330 0 Rhisopus nigricans 220 0 Mucor racemosus A 35 2 Mucor racemosus B 35 2 Oospora lactis 11 0 VIRUS Bacteriophage E coli 6 6 Tobacco mosaic 44 0 Influenza 6 6 PROTOZOA Paramecium 200 0 Nematode eggs 92 0 Chlorella vulgaris algae 22 0 Distance UV intensity cm mW cm e 20 0 7 10 0 25 4 0 50 2 0 100 0 5 200 0 1 300
64. changeable Blocks for TS 100 Optional Blocks Q scs 20 and 12 microtubes Tube volume 0 5 ml and 1 5 ml 32 Thermo Shakers Shakers Incubators Real Time Cell Growth Logger Cat number BS 010120 AK sc 18 02 20 and 12 microtubes 0 2 ml and 1 5 ml BS 010120 CK sc 24 microtubes 2ml BS 010120 EK sc 24n 24 microtubes 1 5 ml BS 010120 GK sc 96A 96 well microplate 0 2 ml for PCR BS 010120 FK scis Interchangeable Blocks for TS 100C Optional Blocks sc 18 20 and 12 microtubes Tube s volume 0 5 ml and 1 5 ml Cat number BS 010143 AK 5 18 02 20 and 12 microtubes 0 2 ml and 1 5 ml BS 010143 CK 5 24 24 microtubes 2ml BS 010143 EK SC 24NC 24 microtubes 1 5 ml BS 010143 GK o SC 96AC 96 well microplate 0 2 ml for PCR BS 010143 FK sc 18 02c Thermo Shakers Shakers Incubators Real Time Cell Growth Logger TS DW Thermo Shaker for deep well plates NEW PRODUCT AM Block 825 eo um Product video is available on the website The block for deepwell plate is mountable thus a custom plate module can be manufactured on request ap Block B 2E SEPARATE BLOCKS TO ACCOMMODATE DIFFERENT DEEPWELL PLATES WILL BE RELEASED FOR EXAMPLE Deep Well Plates NUNC 96 2000 ul Deep Well Plates Axygen 96 0 6 ml Deep Well Plates Eppendorf 96 0 5 ml APPLICATION FIEL
65. ctivation techniques and time of treatment open UV light and UV air recirculator treatment kinetics are presented in the graph Inactivation method efficiency Sample 15 min of UV Air Rec 30 min of UV Air Rec He RR dd nii Air Samples 8496 99 92 100 Surface Samples 40 40 65 95 120 Applications and Articles Calculation of UV dose for each treatment Direct UV Irradiation Cabinet s air treatment BioSan s cabinet features a single open UV lamp 25 Watt germicidal UV irradiation 253 7 nm measurements have been performed and UV intensity were recorded at the level from 20 mW sec cm to 2 mW sec cm at distance to UV source from 2 cm to 50 cm respectively 2 In PCR cabinet volume following UV intensity gradient is formed from 2 mW cm to 20 mW cm Fig 10 UV dosage during treatment UV intensity at specific distance mW cm sec x time of irradiation sec UV dosage during 15 min gradient from 1 800 18 000 mW cm UV dosage during 30 min gradient from 3 600 36 000 mW cm Cabinet s Surface treatment Distance to UV source ranges between surfaces and consequently the UV intensity table 3 Table 3 Average dosage for different surfaces Surface Working surface 40 60 cm Side walls 10 60 cm Front window 10 60 cm UV air recirculation Cabinet s Air treatment Biosan PCR cabinets feature UV air recir culator Recirculator consists of a fan dust filters and
66. culators test report http www biosan lv eng uploads images uvrm 20uvrmi 20article 20eng pdf European Committee for Standardization 2000 European standard EN 12469 Biotechnology Performance criteria for microbiological safety cabinets Web source http nobelprize org Web source http www aircleaners com hepahistory phtml Web source http www filt air com Resources Articles hepa hepa filters aspxistCharacteristics Web source http www who int csr resources publications biosafety Biosafety7 pdf Laboratory biosafety manual Third edition bio Applications Articles UVR M and UVR Mi UV Air Recirculators Test Report 124 Applications Articles UVR M UVR Mi UV air recirculators Test Report UV air recirculators UVR M and UVR Mi produced by BioSan are equipped with bactericidal UV lamps Philips and are used for air disinfection in research laboratories hospitals and veterinary clinics To show the efficiency of UV air recirculators UVR M and UVR Mi we examined UV intensity in Philips 25W bactericidal UV lamps and an impact of UV radiation on various types of microorganisms General information 56 Biotechnomica Photochemical reaction O 2 Medical Biological UV radiation affects the viability of microorganisms by Research amp Technologies causing photochemical reactions in the structure of DNA and RNA Adjacent pyrimidine molecules form di
67. d speed Max continuous operation time 168 hrs Direct drive mechanism Orbit 2mm Overall dimensions W x D x H 220 x 205 x90 mm Weight 2 kg Input current power 12V 280 mA consumption 3 4W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V Platforms for microtest plates IPP 2 standard platform 184 x 132mm for 2 microtest plates Q IPP 4 optional platform 266 x 170mm for 4 microtest plates ORDERING INFORMATION Cat number PSU 2T with stand platform IPP 2 BS 010155 AAG Optional platforms IPP 4 BS 010102 AK RPM Ww fur Mop PSU 2T Mini Shaker biosan 2 Orbit eo ua Product video is available on the website Platform IPP 2 Q Platform IPP 4 Devices Rockers Shakers Rotators Vortexes Vertical rotation 360 eo um Product video is available on the website Bio RS 24 in operation Platform o PRS 22 standard PRS 4 12 4 and 12 up to 50 and 1 5 15 ml 20 30 mm and 10 16 mm Capacity Bio RS 24 Mini Rotator Mini rotator Bio RS 24 provides vertical rotation of the platform The rotator is an ideal instrument for preventing blood coagulation in tubes and for fulfilment of procedures of biological components extraction The device is simple to operate it is designed as a low cost solution Rotator can be used in cold rooms or incubators oper ating at ambient temperature range 4 C to 40 C Lo
68. der 10 min Maintenance of stable temperature during operation User friendly setting of centrifugation parameters speed temperature time and simultaneous display of both set and actual values e Safe operation is provided by a metal protection chamber and a case cover automatic stop at im balance emergency shutdown IMBALANCE dis played and a lock keeping the lid closed while the centrifuge is running e Low noise level Possibility to switch off forced braking e Wide choice of accessory rotors see page 50 Temperature control range 10 C 25 C Stable temperature 25 below ambient to 25 C maintenance range Temperature setting resolution Speed regulation 100 4 200 rpm range for centrifuge tubes 3 370 xg Speed regulation 100 2 000 rpm range for microtitre plates 560 x g Speed setting resolution 100 rpm Rotor imbalance diagnostics automatic stop IMBALANCE warning Slowdown time not more 30 sec Display LCD 2 lines Digital time setting 1 90 min increment 1 min Chamber diameter 335 mm Dimensions 635 x 580 x 335 mm Weight 56 kg Nominal operating voltage 230 V 50 Hz Power consumption 230 V 990 W 4 3 A ORDERING INFORMATION Cat number LMC 4200R without rotors BS 010212 AAA NOILdldSAd D 21412345 P ee Minicentrifuges Vortexes Mini centrifuge Centrifuges Interchangeable Rotors and Accessories for LMC 3000 and LMC 4200R R 2
69. development of samplers and monitoring of DNA RNA amplicon concentration in the air from sterile cabinets microbial quantitative analyses Fig 1 Germicidal shortwave 254 nm ultraviolet energy is used for complete destruction of various biological agents UV intensity Distance mW cm sec cm gt 20 2 25 UV intensity mW cm sec UV intensity needed for decontaminating mW cm sec Distance to UV source cm 116 Applications and Articles Aim of the study The aim of this study is to evaluate the of ciency of UV cabinets produced by BioSan Latvia in comparison to Class Il BioSafety cabinets UV air treatment More than a century has passed since the germicidal effect of UV light was recognized by Niels Ryberg Finsen a Nobel Prize winner in physiology or medicine in 1903 5 and many researches have been performed on UV induced destruction of DNA and microorganisms Low pressure germicidal UV lamps characteristically emit monochromatic low intensity radiation princi pally at 253 7 nm within the germicidal wavelength range as defined by the DNA absorbance spectrum The germicidal UV dose LP UV lamps is calculated as the product of the volume averaged incident irradiance E mW cm and the time of exposure t seconds resulting in units of mJ cm for UV dose 1 Fig 1 Fig 2 Biological agent sizes and filters effectivi
70. dimensions Wx D x 495x410 235 mm Weight 11 8 kg Nominal operating voltage 230 V 50 60 Hz or 120 V 50 60 Hz Power consumption 230 120V 110W 0 5 120 W 1 A WW ORDERING INFORMATION Cat number eo Product video is available on the website LMC 3000 without rotors BS 010208 AAA IX Rotor R 12 15 Rotor R 24GC V Rotors description pictures and catalogue numbers be found on page 50 Minicentrifuges Vortexes Mini centrifuge Centrifuges LMC 4200R Laboratory Refrigerated Centrifuge ut W 45 eo um Product video is available on the website NEW FUNCTION Rotor R 12 10 and icy layer on the chamber walls Rotors description pictures and catalogue numbers can be found on page 50 Laboratory bench top centrifuge with refrigeration LMC 4200R provides temperature control of biomate rial during centrifugation Temperature control of the so called cold shelf is a gold standard for enzymolo gists and cell biologists because it ensures conditions necessary for reproducibility of the sample prepara tion stage Temperature control absence at this stage can cause unpredictable results LMC 4200R is a modern centrifuge designed for oper ation with microtest plates and tubes from 10 to 50 ml FEATURES Effective way of acceleration and deceleration Run up time 20 sec Run down time not more 30 sec o Efficient rate of chamber refrigeration un
71. e Orbital and reciprocal motion Turning angle 0 360 increment 30 Reciprocal motion Rocking angle 0 5 increment 1 Vibro motion Fixed platform tilt angle y Orbit 22mm Platform working area 215 215 mm Non slip silicone mat is supplied as standard Maximum continuous operation time 24 hours Time setting range for 0 250 sec Time setting range for amp 0 5 sec ce mod v Number of cycles 0 125 times N d e Maximum load 1 kg Minksheker Moli Bie Overall dimensions WxDxH 235 235 140mm with platform installed Weight 1 8 kg Input current power consumption 12V 380 mA 4 6 W External power Input AC 100 240 V 50 60 Hz supply Output DC 12V 5 1 21412345 P ACCESSORIES FOR THE STANDARD PLATFORM Optional dimpled mat PDM prevents different size tubes from rolling around the platform ORDERING INFORMATION Cat number Multi Bio 3D with stand platform BS 010125 AAG Bio PP 4S Optional accessories PDM dimpled mat PDM DESCRIPTION SPECIFICATIONS Devices Rockers Shakers Rotators Vortexes PSU 10i Orbital Shaker Shaker PSU 10i provides regulated orbital motion of the platform and is designed for use both in small specialized biotechnological laboratories and in large multidisciplinary laboratories a choice of five 5 interchangeable platforms provides the possibility of performing various procedures and techniques Shaker PSU 10i incorporat
72. e Software has been developed to store display and analyse the data in real time RTS 1 and RTS 1C are compact devices with low profile and small footprint for personal application Temperature control allows to use RTS 1 and RTS 1C as an incubator e g for cell growth RTS 1C has a function of active cooling and temperature profiling via software The ability to change parameters such as temperature RPM and Reverse Spin frequency allows to achieve consistency and reproducibility of results Programmable Cycling profiling of cultiva tion parameters such as temperature RPM Reverse Spin frequency o The ability to remotely monitor the process of cultivation while home or using a mobile phone itis also possible to use other manufacturer tubes of the same type e g Corning 50ml Mini Bioreactor but the device rotor must be modified It is possible to request this specific modification not guaranteed because OS not supported by producer Shaker Incubators Personal bioreactors RTS 1 and RTS 1C Personal bioreactors The dependence of the growth kinetics of E coli BL21 at different temperatures in the 3D format The experiment was conducted on 7 RTS 1C in parallel SOFTWARE FEATURES Real Time cell growth logging 3D graphical representation of OD or growth rate over time over unit Pause option Save Load option Report option PDF and Excel Connect up to 12 units simultaneous
73. ed on pages 58 59 is produced by Grant Instruments Cambridge Ltd Biosan is a sole distributor of Grant Instruments products in Russia CIS and the Baltic States Latvia Lithuania Estonia and an official distributor for a number of other regions A market leading range of versatile high quality dry block heating systems with excellent temper ature control providing a source of precision heating for many sensitive analytical procedures A premium product range at an affordable price Accurate reproducible and safe heating of your samples advanced temperature con trol combined with high quality precision engineered blocks providing excellent thermal contact Versatile range of interchangeable heating blocks to fit any tube or plate you are using for your samples Full range of models and options to cater for ba sic through to more sophisticated applications Wide range of accessories Al Se Accessories gt QBD4 with a lid Model Cat Num QBD1 QBD2 QBD4 QBH2 QBA1 QBA2 Type Digital Digital Analogue Number of blocks 1 2 4 2 17 2 Temperature range amb 5 C to 130 C amb 5 C to 200 C amb 5 C to 100 C Temperature setting range 15 C to 130 C 15 C to 200 C 15 C to 100 C Temperature stability 37 C 0 1 0 1 1 a within 40 1 40 1 Display Resolution LED 0 1 LED 0 1 C n a Safety Overtemperature Thermal fuse Timer with a sound alarm 1 min
74. ed otherwise QUALITY The Biosan Quality Management System complies with the requirements of LVS EN ISO 9001 2009 the scope of supply is development production sales and service of laboratory equipment High quality customer service and readiness to meet ever growing customer requirements to modern equip ment are the main goals of ISO 9001 2009 compliance certified since 2004 GUARANTEE AND AFTER SALES SERVICE Biosan equipment is reliable designed and built to provide years of trouble free service Most Biosan equipment is guaranteed for two years against faulty materials and workmanship Warranty terms and condi tions are indicated in the product manual All Grant standard laboratory equipment is guaranteed for three years against faulty materials and workmanship Local distributors and service centres provide necessary tech nical assistance within and outside the warranty period Biosan technical support team provides direct support offering the best solution for assistance upon receipt of request via e mail service biosan lv or forms available at Technical Support section of Biosan web site WORLD WIDE AVAILABILITY AND SUPPORT FOR BIOSAN LABORATORY EQUIPMENT Biosan laboratory equipment and specialist technical support is available world wide Please visit multi lingual English German Italian Latvian Spanish Russian web site http www biosan lv for further product information videos brochures manuals ar
75. efficiency 0 1x25 W efficiency gt 99 per 1 hour gt 99 per 1 hour 1xTLD 15W 1xTLD 30W 4mm 4mm 2mm 4mm 8mm 8mm 4mm 4mm 5mm 92 95 gt 99 90 Polymethyl gt 96 methacrylate UV protection film type 4 MIL CLEAR ALTUGLAS EX 650x475 mm 1200x520 mm Automatic open UV lamp switch off when screen is open Inlet for power cords Inlet for power cords or 1 Built in socket max 1 000 W pls order respectively 100 240 V 50 60 Hz 67 W 690 x 535x555 mm 690 x 585 x 555 mm 23 33 kg 28 8 39 kg 31 2 42 kg T 4 WxDxH 800x600x745 mm 3 Built in sockets max 1 000W 230 V 50 Hz or 120 V 60 Hz 315 VA 1 4 A 530 VA 4 5 A 1245 x 585 x 585 mm 58 68 5 kg T 4L WxDxH 1290 x 600 x 770 mm UV intensity mW cm sec Distance to UV source cm Yeast e Saccharomyces cerevisiae e Brewer s yeast DNA RNA UV Cleaner Boxes Germicidal shortwave 254 nm ultraviolet energy is used for complete destruction of various biological agents per 1 second UV intensity needed for decontaminating mW cm sec Bacteria Clostridium tetani e Mycobacterium tuberculosis Salmonella Dysentery bacilli e Staphylococcus aureus Streptococcus hemolyticus Average dosage for different surfaces Surface Working surface 40 60 cm Dosage after 15 min 1 800 2 700 mW cm2 Viruses Bacteriophage E coli Influenza per 15 3
76. egrated Grade II dispensing pump e Recirculation system Model specific modules e Labaqua HPLC Point of use microfilter Labaqua HPLC with TOC monitor Point of use microfilter Integrated TOC monitor Labaqua BIO Point of use ultrafilter UV sterilization module Purified water specifications Pure Grade 2 water resistivity Pure Grade 2 water conductivity Ultrapure Grade 1 water resistivity Ultrapure Grade 1 water conductivity TOC RNase DNase Bacteria Endotoxins Particles gt 0 22 um Nominal flow pure water to storage tank Nominal dispense flow ultrapure water Nominal dispense flow pure water Deionization module life standard module Recovery Dimensions WxDxH cm Storage tank Tank dimensions WxDxH cm Feed water pressure Feed water conductivity Labaqua HPLC gt 10 x cm 0 1 uS cm 18 2 MO xcm 0 055 uS cm 2 ppb N A N A 1 cfu mL lt 0 15 EU mL lt 1 per mL 10 L h 2 L min 4 L min 1m 3096 50x40x60 251 30 25 50 0 5 5 bar lt 900 uS cm Labaqua Bio gt 10 x cm 0 1 uS cm 18 2 MO x cm 0 055 uS cm 2 ppb 0 01 ng mL 4 pg uL 1 cfu mL 0 001 EU mL 1 per mL 10 L h 2 L min 4 L min 1m 3096 50x40x60 251 30 25 50 0 5 5 bar lt 900 uS cm biosan Labaqua Bio ORDERING INFORMATION Labaqua Bio Labaqua HPLC Labaqua HPLC wi
77. els from basic to advanced with digital controls Proven performance technology to deliver temperature control you can rely on Set and Forget technology minimal bath setup maximum time for your work More information about offered Grant Instruments products can be found on Biosan web site www biosan lv Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heatina cooling Circulators SUB Aqua Pro Digital Unstirred Water Bath In Russia CIS and Baltic countries Latvia Lithuania Estonia this type of water bath will be available under Biosan Grant WB Plus label Built to the highest standard and specifications and incorporating the latest technology the SUB Aqua Pro advanced water bath range supports even the most demanding applications requiring accurate temperature control Choose from eight models with base tray and lid included as standard e Ambient 5 C to 99 C operation Set and Forget technology fast heat up accurate temperature control Stability 0 2 e Adjustable over temperature alarm protect samples from over heating Advanced dry start and run dry protection e Three programmable temperature presets e 3 year warranty SUB Aqua Pro digital unstirred water bath range summary of specifications SAP5 SNOIIVOIJID3dS P NOlLdlH2S3G P
78. ement bar SU Iran Stirring liquid viscosit up to 1 170 mPa s y Maximum continuous operation time 12 hrs Operation in closed laboratory rooms at ambient temperature from 4 C to 40 C Working plate size 110x110 mm 160 mm Weight 0 8 kg MMS 3000 MMS 3000 SNOILVOIJID3dS PP DESCRIPTION a Magnetic Stirrers Overhead Stirrer MSH 300 and Intelli Stirrer MSH 300i Magnetic Stirrers with hot plate MSH 300 and Intelli Stirrer MSH 300i are magnetic stirrers of the new generation Enclosures of stirrers are made of metal painted with powder enamel chemically resistant to acids and alkali The stirrers are equipped with a detachable stand for supporting various sensor elements temperature pH and others inside the stirred liquid The stirrers are supplied with a cylinder shape magnetic stir ring bar 6x 25 mm for universal use covered with Teflon Units are equipped with the overheat protection providing an automatic switch off of the device when overheating for the set temperature difference occurs Magnetic stirrers with heating can be used for laboratory operations such as organic synthesis extraction analysis of oil products pH measurements dialysis soil suspending preparing buffer solutions etc Additional protection disables the heating if the temperature of plate exceeds the set temperature for 30 C Operation temperature range 4 C to 40
79. emperature regulation range Temperature control range With external probe Display Temperature uniformity on the plate Working plate heating time till 330 C Diameter of working plate Plate material SR 1 attachable stand size Length of magnetic stirring element Max stirring liquid viscosity Fault indication Overall dimensions W x D x H Weight Nominal operating voltage Power consumption Stirring Power consumption Heating Magnetic Stirrers with hot plate MSH 300 Intelli Stirrer MSH 300i 250 1 250 rpm 100 1 250 rpm 10 rpm increment 15 litres 20 litres 30 330 30 C 330 C 1 C increment 20 C 4150 C END 3 15 min 11 160 Aluminium alloy 08x320mm 10 50 mm d 20 70 mm E up to 1 170 mPa s Outputs sound signal and turns off the heating 2 9 kg Outputs an error code on the display turns off the heating 190x270x100 mm 3 2 kg 230 V 50 60 Hz or 120 V 50 60 Hz 8 5 W 550 W PLATE HEAT UP TIME FOR MSH 300 Connecting external probe to the Intelli Stirrer MSH 300i PLATE HEAT UP TIME FOR INTELLI STIRRER MSH 300I DPMD Double clamp with holder for external probe External probe SKM2 Clamp DPMD Double clamp SR 1 stand SNOILVOIJID3dS P DESCRIPTION SPECIFICATIONS h Magnetic Stirrers Overhead Stirrer MM 1000 Overhead
80. ems for measurement of absorption at the definite wavelength as well as for quantitative estimation of concentration of colour solution absorbing green light The operation principle is based on measurement of optical density with digital presentation of results in McFarland units The unit is calibrated at the factory for operation with 18 mm diameter glass tubes and keeps calibration without power supply However if necessary it is possible to calibrate the unit by 2 6 points in 0 5 5 0 DEN 1 and 0 0 6 0 DEN 1B McFarland unit range Both commercial standards e g produced by BioMerieux Lachema etc and the cell suspensions prepared in a laboratory can be used for calibration DEN 1 Following calibration kits are available on request e CKG16 for glass tubes with diameter 16 mm e CKG18 for glass tubes with diameter 18 mm Up to date information on calibration kits is can be found on the website http www biosan lv Two versions of the product are available 1 DEN 1 powered from external energy supply 2 DEN 1B powered both from external energy supply and from batteries AA Besides DEN 1B operates with higher precision of measurements up to 0 01 McF DEN 1B rear side with calibration controls Power DC 12V Calibration e gt 2 er n instal act Application of DEN 1 for determining concentration of microbial cells of supernatant in tubes during centrifugati
81. equipment up to date brochures user manuals VISION We are planning to continue constant development and improvement to be on the leading positions of fast emerging World of Biotechnomica It allows us to open new opportunities and to enter new markets thus staying in one line with the leading bioengineering companies in the European Union will be happy if you are interested in Biosan products Thank you for the cooperation Vasily Bankovsky Doctor of Biological Sciences R amp D director Chairman of the Board at Biosan Table of content NEW PRODUCTS AND ANNOUNCEMENTS RTS 1 RTS 1C MPS 1 HiPo MPP 96 TLC S TS DW S Bt CVP 2 Labaqua Series MIXING DEVICES ROCKERS SHAKERS ROTATORS VORTEXES Rockers MR 1 MR 12 Shakers 3D Multi Bio 3D PSU 10i PSU 20i MPS 1 PSU 2T Rotators Bio RS 24 Multi Bio RS 24 Multi RS 60 Vortexes V 1 plus V 32 MSV 3500 THERMO SHAKERS Thermo Shakers PST 60HL PST 60HL 4 PST 100HL TS 100 TS 100C TS DW Shakers Incubators ES 20 ES 20 60 Personal bioreactors RTS 1 amp RTS 1C MINICENTRIFUGES VORTEXES MINI CENTRIFUGE CENTRIFUGES Minicentrifuges Vortexes FV 2400 FVL 2400N MSC 3000 MSC 6000 CVP 2 Minicentrifuge Microspin 12 Centrifuges LMC 3000 LMC 4200R THERMOSTATED EQUIPMENT THERMOSTATS DRY BLOCK HEATING COOLING SYSTEMS Thermostats Dry Blocks Bio TDB 100 TDB 120 QB series Dry block Thermostats CH 100 CH3 150 THERMOSTATIC EQUIPMENT WAT
82. er 210mm Overall dimensions 285 x350x 190 mm WxDxH Weight 6 15 kg Input current power consumption 24V 750 mA 18W External power Input AC 100 240 V 50 60 Hz supply Output DC 24 V eo um Product video is available on the website NEW PRODUCT Adapters AP 96 for 96 well semi skirted and non skirted PCR plates Adapter AP 384 for 384 well PCR plates ORDERING INFORMATION Cat number 2 BS 010219 A02 with rotor for two PCR plates protection lid and adapters AP 96 a set of 2 adapters for 96 well semi skirted and non skirted PCR plates Optional accessories AP 384 BS 010219 EK2 A set of 2 adapters for 384 well PCR plates Adapters are made of Ertacetal C Autoclavable Minicentrifuges Vortexes Mini centrifuge Centrifuges High speed Mini centrifuge Microspin 12 eo um Product video is available on the website Protection lid NEW FUNCTION Built in rotor MSR 12 with protective lid that allows to work not only with microtubes but also with spin columns ap A 02 Adapters ec e A 05 Adapters High speed mini centrifuge Microspin 12 is a compact desktop centrifuge designed for biomedical laboratories Microspin 12 is used for extracting RNA DNA samples sedimentation of biological components biochemical and chemical analysis of microsamples A display simultaneously shows actual and set values for 1 Centrifugation time 2 Set and
83. es a direct drive system a brushless motor with a guaranteed service life up to 35 000 hours and an automatic loading balancing system These innovations allow for continuous mixing up to 7 days ensure reliable trouble free operation for more than 2 years and significantly expand the range of the device performance in both high and low limits Shaker can be used in cold rooms or incubators oper ating at ambient temperature range 4 C to 40 C O 10 mm BB Product video is available on the website IX Speed control range 50 450 rpm Platform Bio PP 4 increment 10 rpm max speed depends on the load and vessels shape Digital speed control Max continuous 168 hrs operation time Orbit 10 mm Digital time setting 1 min 96 hrs non stop Maximum load 3 kg Overall dimensions 220 x 205 x90 mm WxDxH Weight 3 4kg Input current power 12 V 800 mA 9 6 W consumption External power Input AC 100 240 V supply 50 60Hz Output DC 12V ORDERING INFORMATION Cat number PSU 10i without platform BS 010144 AAN Optional accessories UP 12 with silicone mat BS 010108 AK Bio PP 4 with silicone mat BS 010116 AK P 6 250 BS 010108 DK P 12 100 BS 010108 EK P 16 88 BS 010116 BK Description and pictures of all platforms can be found on page 14 Devices Rockers Shakers Rotators Vortexes PSU 20i Multi functional Orbital Shaker O 20 mm Orbit 3 mU Platform PP 20 4 Multi rotation Motio
84. eters also determine the angular speed of rotating Vortex Layer VL and tran sition state from the Irrotational Vortex IRV when angular speed of the VL is proportional to the radius to the Rotational Vortex when the angular speed of the VL is the same and VL looks like a monolithic Vortex cavity Common rules regulating Vortex type mixing processes may be stated as follows the more time has passed since Vortex formation the more obvious is a transition from IRV to the RV The concept of the Reverse Spin mixing is based on these assumptions Reverse Spinning vs Orbital Shaking Applications Articles Noninvasive Vortex Mixing Principle Spread of the broth media inside of rotation tube as a function of rotation intensity Vortex Cave Broth media e 250 min 1 000 min 2 000 min Rotation Rotation Intensity around axis Symmetrical vs Asymmetrical broth media distribution Reverse Spinning t ah e Fits any diameter of the rotating vessel Natural centric auto balancing Simplicity No power consumption for contra balancing Self cleaning optical cells Orbital Shaking Features Proportionality between orbital diameter and the diameter of the moving vessel Artificial hula hoop auto balancing Complexity Extra power consumption for contra balancing Fig 1 Aeration effect on E coli BL21 growth using different growth techniques 4 a5 iM 850 nm 3
85. f mechanical air contamination in cabi Microbial contamination in laboratory air and nets of two types PCR cabinet UVC T M AR sterile cabinets Quantitative results of microbial BioSan and laminar flow cabinets BioSafety air contamination in cabinets of two types PCR class Il cabinet prototype by BioSan and BSC Il cabinet UVC T M AR BioSan and laminar flow cabinet ABS Cabinet Class Il by Microflow as cabinets BioSafety class Il cabinet prototype by the positive control laboratory air samples were BioSan and BSC cabinet ABS Cabinet Class II by taken Fig 5 Microflow as the positive control laboratory air samples were taken Fig 6 Fig 5 Mechanical contamination Fig 6 Microbial contamination 0 3 um particles 0 3 um particles n x 10 0 Legends for figures 5 and 6 Positive control laboratory air O Uv Cabinet UVC T M AR Biosan Latvia Laminar flow cabinet HEPA BSC II Cabinet prototype Biosan Latvia BSC Il Cabinet ABS Cabinet Class Il Microflow UK Applications and Articles Amplicon contamination inactivation efficiency Results analysis Real time PCR ensures product quantifi cation using four standards of different Lambda phage DNA concentration and comparing Ct Cq values of samples to those of concentration stand ards based on standard curve Fig 8 see Corbett Research Rotor Gene 3000 manual for more information Following
86. g an innovative SMS algorithm for performing the PCR analysis allowed us to obtain a high expert evaluation from the leading Life Sciences laboratory in the Baltic Among the new products for biosafety there is the long awaited laminar cabinet providing thermostatting of the laminar flow TLC S see page 93 that means the active control of temperature shelf so necessary when working with cell cultures and experimental animals In 2016 among the new products in the field of cellomics the new CO2 thermostat incubator S Bt Smart Biotherm will appear see page 6 designed to work with cell cultures where it is necessary to maintain a given concentration of carbon dioxide CUSTOMER SUPPORT Our team of highly skilled specialists provides prompt solutions in questions of product ordering operation and maintenance We advise our customers on the stage of equipment ordering and pay attention to all requests and wishes Company s professionals provide solutions to the problems occurring during operation and maintenance of equipment as well as quickly and efficiently solve the issues of the warranty and post warranty service We always encourage our customers to visit our production department and complete the training for operation and maintenance of our products Additional information about the products can also be found on the website www biosan lv in 7 different languages including product videos that demonstrate functional characteristics of the
87. growing gel washing soft extraction and homogenisa tion of biological components in solutions Multi Bio 3D provides realization of several types of motion in one module This option of Biosan instruments essentially extends possibilities and enhances efficiency of preparation of test samples as well as allows selecting the mixing type according to individual requirements NOILdId SAC P Microprocessor control allows performing not only Orbital 3D rotation of the platform but also Reciprocal 3D motion of ping pong type as well as Soft vibrating rocking These three motion types be performed separately pairwise and in cycles peri Mini haker Multi Bio 30 piofan odically repeating the sequence of three motion types The shaker is designed for laboratories with increased demands for quality of mixing extraction and cell growing processes 3 Pr ideo is available on the website 7 Non slip temperature resistant silicone mat located on the shaker platform provides stable position for vessels during shaking Optional dimpled PDM mat fixes tubes Nro if 1 Y of different sizes 6g xe 22 mm Programmable shaker can be used in cold rooms or 3 2 Orbit i incubators operating at ambient temperature range 3D Multi rotation 3D uBi rtstion 44 C to 40 C Rocking uni rotation Speed 1 100 rpm Multi Bio 3D with PDM mat control rang
88. hermo Shakers Shakers Incubators Real Time Cell Growth Logger TS 100 Thermo Shaker Thermo Shaker TS 100 provides intensive mixing and temperature control of samples in microtest tubes or PCR plate Functions of heating up to 100 C and mixing can be performed both simultaneously and independently i e the unit implements three devices in one Shaker e Dry block Thermostat Thermo Shaker TS 100 is used for DNA analysis sample preparation for extraction of proteins polysaccharides lipids and other cellular components Features of TS 100 meet the increased requirements of the user including e Quickly reaches the set mixing speed and maintains same amplitude of rotation around the block Stable maintenance of the temperature of a wide range over the entire surface of the block e LCD display shows the set and actual temperature speed and time e Quiet engine operation compact size long service life Heating source is a printed heating board 12 V Mixing is provided by movement of orbital type The instrument is applicable in e Genetic analysis in extraction of DNA RNA and further sample preparation Biochemical study of enzymatic reactions and processes e Extraction of metabolites from cellular material Temperature setting range 25 C 100 C Temperature control 5 C above ambient 100 C range Temperature setting resolution 0 1 Temperature stability 0 1 Temperature acc
89. icrobiological filter Aspirator with trap flask FTA 1 is designed for aspiration removal of alcohol buffer remaining quantities from microtest tube walls during DNA RNA purification and other macromolecule reprecipitation techniques The device can be used also for routine operations of cells washing from culture medium and resuspension in buffer Aspirator operation principle is based on creating negative pressure in trapping flask using built in micro compressor The collecting tip is connected with polyeth ylene tube to the trapping flask Liquid is removed from the microtest tube when the collecting tip touches the solution surface A tube holder organizer is conveniently located at FTA 1 right hand side it accommodates two tubes e g for hydrochloric acid solution and distillate necessary for collecting tip washing and storing so that a tip can be re used max level OQ suction microbiologicalhydrophobicfiltertype2200 02 Suction microbiologic filter eliminated risk of contami nation with bacteria viruses and infected particle from patient to suction pump or central vacuum distribution Suction microbiological filter is hydrophobic with very high bacterial efficiencies up to 99 99999 particles bigger than 0 027 micron which is smaller than Hepatitis A Band C Applications Suction filter located behind output of the jar in front of the vacuum source The filter put on so as the side of filter marked IN
90. ientific community would be also of interest for you particularly if it would serve as yet another starting point for the development of innovative biotechnologies and appearance of new planets and their satellites in the sky of the World of Biotechnomica Sincerely Vasily Bankovsky Ph D Biology Head of R amp D Department Biosan Chairman of the Board Applications Articles 109 The World of Biotechnomica 110 Applications and Articles Reverse Spin Technology Innovative Principle of Microbial Cultivation n Medical Biological Research amp Technologies Authors V Bankovsky l Bankovsky P Bankovsky J Isakova l Djackova Sharipo A Zhukov Di lers R Rozenstein V Saricev S Djacenko V Makarenko U Balodis This paper presents theoretical and experi mental studies of the microorganism growth using Reverse Spin Technology RST Reverse Spinner is a thermostating device with very low energy consumption that realizes innova tive type of mixing where liquid cells in liquid medium is mixed by the tube rotation around its axis leading to highly efficient Vortex Type Mixing VTM Present work is the first to show experimental results of cell growth kinetics obtained by using disposable falcon tubes agitated on a principle of RST Growth conditions for several model microorganisms facultative anaerobe E coli BL21 extreme aerobic microorganism
91. is the highest value of the maximal speed for magnetic stirrers of global producers Strong magnets hold the driven magnetic element firmly in the magnetic clutch Stirring is performed without undesir able heating and noise Enclosures of stirrer MS 3000 are made of strong steel and painted with powder enamel which is chemically resistant to acids and alkali The stirrers are supplied with a cylinder shape magnetic stir ring bar 6x 25 mm encapsulated in PTFE for universal use MMS 3000 is equipped with a detachable stand for supporting various sensor elements temperature pH and others inside the stirred liquid Magnetic stirrers are ideal laboratory instruments for PH metering extraction and dialysing with the small quanti ties of substances Operation temperature range 4 C to 40 C from cold rooms to incubators at maximal relative humidity 80 CATALOGUE NUMBER Cat number MS 3000 BS 010301 AAF MS 3000 blue on request BS 010301 ABF MMS 3000 BS 010305 AAF Optional accessories for MMS 3000 SKM2 Clamp see page 79 BS 010309 AK DPMD Double clamp see page 79 BS 010309 CK MS 3000 MS 3000 MMS 3000 with a stand Magnetic Stirrers Overhead Stirrer MS 3000 and MMS 3000 Magnetic Stirrers MS 3000 MMS 3000 Speed control range 0 3 000 rpm Working surface material Stainless steel SR 1 attachable stand size 08x 320 mm Max length of magnetic stirring el
92. ith non slip silicone mat Universal platform for different flasks Clamps ordered separately Clamp for 100 250 500 1000ml flask for UP 168 Four level flat platform with non slip rubber mat Three level flat platform with non slip rubber mat Two level flat platform with non slip rubber mat One level flat platform with non slip rubber mat Platforms for PSU 20i and ES 20 60 Dimensions Working Area 345 X 430X105 300x400x 80 mm mm 360 400 mm 360 X 400 mm 360 X 400 mm 360 xX 400 mm 360 X 400 mm 360 X 400 mm 360 X 400 mm 360 xX 400 mm 360 X 400 mm 400 360 mm 360 x 400 mm 360 X 400 mm 65mm 85mm 105mm 130mm 380 480X510 365 X 465 X510 mm mm 380 x 480 340 365 465 x 340 mm mm 380 x 480 170 365x 465X170 mm mm 380 X 480 mm 365 X 465mm Cat number BS 010145 AK BS 010135 BK BS 010135 CK BS 010135 AK BS 010135 DK BS 010135 FK BS 010135 JK BS 010126 HK BS 010126 JK BS 010126 LK BS 010126 IK BS 010126 EK BS 010126 DK BS 010126 CK BS 010126 BK 16 DESCRIPTION Mixing Devices Rockers Shakers Rotators Vortexes MPS 1 Multi Plate Shaker SHAKER FOR VARIOUS APPLICATIONS Platform for 24 tubes 1 5 2 ml Microtest plate 200 ul High Speed Multi Plate Shaker MPS 1 can be used in virtually any application by providing adjustable mixing of reagents in microtest plates PCR plates deepwell plates and test tubes shaking tubes 0 2 to 2 m
93. kes the process of growing cells and controlling their concentration very difficult to reproduce The problem lies in the fact that the turbidimetric coefficients unlike molar extinction coefficients are not linear The behaviour of light in dense cell suspensions see fig 5 is very interesting and at more than 2 OD at 600 nm it is almost impos sible to measure the concentration of cells directly unless you measure the Rayleigh scattering We approached this problem from a different side It is known that the shorter the optical path is the more accurately it is possible to measure the concentration of cells even at high densities up to 10 OD For this purpose test tubes containing different volumes of medium are intensely rotated 2 000 min and as a result a monolayer of medium is generated which thickness is directly propor tional to the volume of culture medium in the tube see fig 2 below Previously we achieved linearity in the data when measuring cell concentrations from 1 10 OD in the optical path of 1 mm there fore correction coefficients were introduced in the program of RTS 1 which allows to measure concen trations of cells in a wide range The algorithm for determining the concentration of bacterial cells in Real Time includes the formation of the monolayer at given intervals and cell concentration measuring process The process takes 5 10 seconds and then initially set parameters for the cell growth au
94. l and vortexing any volume up to 50 ml The shaker is compact and user friendly The shaker is idealfor personal use MPS 1 features a head for vortexing a single tube Shaker can be used in cold rooms or incubators operating at ambient temperature range 4 C to 40 C Low voltage external power supply 12 V provides electrical safety in humid environment MPS 1 features Pulse Mode mixing function that works on the principle of giving a periodic impulse the tube is accelerated to the set speed holds it for 3 seconds and then drops the speed to zero This motion is repeated until the timer runs out This method provides a constant state of resuspension of the particles inside a tube as the acceleration is always changing The advantage of this method is the high throughput of mixed samples compared to vortexing a single tube Deepwell plate 1000 ul Platform for 32 tubes 0 5 ml FEATURES NEW PRODUCT Semi unskirted PCR plate Deepwell plate 500 ul Speed control range 300 3 200 rom e Smooth mixing with 3 mm orbit e Mixing presets Pulse Mode mixing function e Quiet operation low noise at maximum speed High stability stationary at maximum speed Universal platform holder for Deepwell plates and Microtest plates e Additional platforms for semi and unskirted PCR plates 200 ul as well as for tubes from 0 2 to 2 ml e Can be used in cold rooms or incubators temperature range
95. l for growing cells and cell cultures in disposable plastic reactor bags working volumes up to 10 liters media volumes up to 5 liters Rocker Shaker can be used in cold rooms or incubators operating at ambient temperature range 4 C to 40 C Low voltage external power supply 12 V provides electrical safety in humid environment Mixing frequency range 1 99 oscill min increment 1 oscill min Tilt angle range for 1 50 oscill min 0 10 increment 1 Fixed tilt angle for 51 99 oscill min 10 Digital time setting 1 min 99 hrs 59 min increment 1 min non stop Display LCD 16 x 2 signs Maximum continuous operation time 168 hrs Maximum load 5 kg Platform working area 480 x 380 mm Platform with non slip rubber mat is included in standard set Overall dimensions W x D x H 430 x 480 x 210 mm Weight 11 9 kg Input current power consumption 12V 1 1A 13W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V ORDERING INFORMATION Cat number MR 12 with standard platform PP 480 BS 010130 AAI NOILdId SAC P 5 1 21412345 P DESCRIPTION SPECIFICATIONS Devices Rockers Shakers Rotators Vortexes 3D Sunflower Mini Shaker Sunflower 3D Mini Shaker provides adjustable three dimensional smooth rotation of the platform andis designed for mixing blood samples for minigel staining and destaining sample washing blot hybrid ization reactions Mini Shaker is a compact device with low energy
96. l models from the TC120 upwards are suitable for use as both open and closed loop circulators i e remote vessel open or closed For more powerful heating requirements above 200 C contact marketing biosan lv for advice Grant Model selection see 71 Any of the four Grant Optima digital thermostats can be combined with any of eight Grant tanks five stainless steel and three plastic to provide a choice of 32 models Heating Circulators Specifications on page 70 and all available accessories on page 71 NOILdId SAC P Thermostatic equipment Water Baths Orbital linear shaking Baths 70 Unstirred Water Baths Heating cooling Circulators Optima Series Heating Circulators Specifications TC120 a Grant Optima Heating Circulators Specifications Z Stability DIN 12876 i err c Uniformity DIN 12876 LL 70 C U Setting resolution iG CL Display Un Timer function No preset temperatures Two point re calibration Offset adjustment Socket for external probe TXPEP TXSEP Communication interface Programmable Relays Safety overtemperature fluid level float switch Alarms can be configured to switch a relay Heater power 230 V kW Electrical power 230 V kW Height above tank rim mm Depth below tank rim mm Grant Optima thermostat pumps integral Maximum pressure water mbar Maximum flow water L min Pipe bore inlet outlet mm Dimensions H x D
97. logy and laboratories working with cell cultures Besides HEPA filtered sterilie airflow the cabinet has two more additional options 1 Highly efficient filtration is provided by two pleated HEPA filters that meet the requirements of protection class H14 Filters installed in a cabinet are effective not less than 99 995 if trapping particles of 0 3 micron In a standard mode the inflow air enters through the perforated front grille without penetrating into the working area The recirculated air in the working area enters the grille in the perimeter of the working area All the air then flows upwards where 70 of the air is recirculated through the main HEPA filter and is directed to the working area as a laminar flow while 3096 of the air is directed out of the cabinet through the second HEPA filter 2 Termostated laminar flow of sterilized air 3 Recirculation systems with ultraviolet light irradiation to inactivate viral particles DNA and RNA amplicons in the recirculated air ANNOUNCEMENT HiPo MPP 96 Microplate Photometer INFORMATION ABOUT THE AVAILABILITY OF THE DEVICE WILL BE PUBLISHED ON OUR WEBSITE WWW BIOSAN LV Features of QuantAssay software e ELISA assays of any complexity can be carried out via robust assay editor e Multiplex assays up to 7 assays on one plate e Qualitative assay includes up to 8 controls e Quantitative assay includes up to 20 standards e BestFit function for selecting the
98. lti Spins MSC 3000 1 000 3 500 rpm 800xg 1 sec 99 min Soft medium hard 0 20 sec increment 1 sec 1 999 cycles LCD 2x 16 signs Autostop at open lid 190x235x125 mm 2 1 kg 12V 11 W 0 9 A Input AC 100 240 V 50 60 Hz Output DC 12V ORDERING INFORMATION 5 3000 with standard rotors R 1 5 R 0 5 0 2 MSC 6000 with standard rotors R 1 5 R 0 5 0 2 Optional rotors Capacity R 0 5 0 2 12 x0 5 ml and 12 x0 2 ml microtubes 24 R 1 5 121 5 ml microtubes 12 O n 2 0 5 8x2 0 ml and 8x0 5 ml microtubes 16 n 2 0 5 0 0 6 2 0 6x0 5 ml and 6x0 2 ml microtubes 18 O sn 16 Two 8 section strips for 0 2 ml microtubes 16 sn 32 Four 8 section strips for 0 2 ml microtubes 32 not compatible with Multi Spins produced before 2015 95 22 05 02 MSC 6000 By 1 000 6 000 Uv rpm 2 350x m d 1 sec 30 min H Z v Lid lock 2 5 kg 24 V 24 W 1 A Input AC 100 240 V 50 60 Hz Output DC 24 V Type Standard Standard Optional Optional Optional Optional Cat number BS 010205 AAN BS 010211 AAL Cat Number BS 010205 BK BS 010205 AK BS 010205 CK BS 010205 DK BS 010202 AK BS 010205 FK Minicentrifuges Vortexes Mini centrifuge Centrifuges CVP 2 Centrifuge vortex for PCR plates After many years of Combined Centrifuge Vortex concept success we are proud to introduce the long awaited Centrifuge Vortex for PCR plates CVP 2 to the sample
99. ly Remote monitoring option requires internet connection Cycling Profiling options User manual calibration options for any microorganism Growth Kinetics and Growth Rate graphs of E coli BL21 OD overtime Growth rate ae 10 20 36 40 50 68 7D 80 80 100 110 120 130 146 150 I0 70 40 50 69 70 820 90 100 110 120 130 160 hours Hars TYPICAL APPLICATIONS e Bacterial cultivation with real time growth kinetics e Strain screening e Temperature stress fluctuation experiments e Media screening and optimization e Synthetic and systems biology e Inhibition and toxicity tests Strain quality control Growth Kinetics graph of E coli BL21 in 3D ORDERING INFORMATION Cat number RTS 1C including TubeSpin Bioreactor 50 TPP 20 pcs BS 010160 A04 RTS 1 including TubeSpin Bioreactor 50 TPP 20 pcs BS 010158 A04 Optional accessories TubeSpin Bioreactor 50 TPP 20 pcs BS 010158 AK USB 2 0 Hub 10 x ports BS 010158 BK gt ic ate aw Y 4 d ww 2 vL h n A Ta Ts MINICENTRIFUGES VORTEXES MINI CENTRIFUGE CENTRIFUGES DESCRIPTION Minicentrifuges Vortexes Mini centrifuge Centrifuges FV 2400 Microspin Mini centrifuge vortex Micro Spin FV 2400 is specially designed for genetic engineering research for PCR diagnostics experi ments Units can be used in microbiological biochemical clinical laborat
100. ly after making sure they do bring the needed sample preparation solution WORLD OF BIOTECHNOMICA In 2005 Biosan put forward the concept of the World Biotechnomica see page 108 following which we are ready to offer you not only already known devices but brand new unique equipment The orbits of the World Biotechnomica were filled by the new satellites Reverse Spinner RTS 1 and RTS 1C see page 38 personal bioreactors which provides logging of microbial growth in real time RTS 1 and RTS 1C provide reproducibility of cell preparation cryopreservation obtaining cell material in a given phase of growth etc and facilitate reproducibility of manipulations with complex biological structures which are the cells of micro and macro organisms The next generation of mini bioreactors is the development of a matrix bioreactor a multichannel detector of cell culture growth concentration Smart Platform intended to control the optical density in the Erlenmeyer Flask We also pay attention to the development of conventional diagnostic techniques The product line for enzyme linked immunosorbent assay ELISA has been replenished with a new product the Microplate Photometer Hypo MPP 96 see page 98 Its reliability of measurements and the data processing program are approved by appropriate external laboratory tests On the orbit of Genomics there is a new device for Life Sciences market the centrifuge vortex for PCR plates CVP 2 see page 46 Usin
101. m Weight 1 7 kg Monina operim 120 or 230 V 50 Hz 120 or 230V 60 Hz voltage Power consumption 30 W 0 27 A 30 W 0 27 A 120 230 V 30 W 0 13 A 25 W 0 1 A ORDERING INFORMATION Cat number FVL 2400N with standard rotors BS 010202 AAA R 1 5 and R 0 5 0 2 Optional rotors see table below not compatible with Combi Spins produced before 2015 20 5 0 2 5 22 05 2 0 5 0 2 sr 16 5 SNOILVOIJID3dS P NOlLdluos3a P Gy Minicentrifuges Vortexes Mini centrifuge Centrifuges MSC 3000 and MSC 6000 Multi Spins or MSC 6000 gt Centrifuge vortex Multi Spins MSC 3000 and MSC 6000 are N m products of many years evolution of Spin Mix Spin technology that is intended for collecting micro volumes of reagents on the microtube s bottom first centrifugation spin following mixing mix and collecting the reagents again from the walls and cap of the microtube second spin We named this repetitive algo rithm of operation that is aimed at reducing the mistakes during sample preparation for PCR analysis a sms algorithm Multi Spin is a fully automatic device for reproducing sms algo rithm for 12 tubes at one time thus saving time considerably A must have instrument for PCR and DNA analyses laboratory Multi Spin is four devices combined in one DESCRIPTION 1 Centrifuge Maximum RCF MSC 3000 up to 800xg MSC 6000 up to 2 350xg 2 V
102. m 100 515 TCI20 STS TX150 STS 300 150 150 mm 0 to 120 C 0 to 150 C Ww 180 mm 330 x 180 x 395 mm ST12 12L 205 x 300 mm T100 ST12 TC120 ST12 TX150 ST12 stainless steel 4 5 kg 85 140 mm 0 to 100 C 0 to 120 C 0 to 150 C ye mm 325 300 150 mm 5 mm _w 330mm 360 x 330 x 395 mm _ ST18 18L 385 x 300 mm T100 ST18 TC120 ST18 TX150 ST18 Stainless steel 7 kg e 75 130 mm 0 to 100 C 0 to 120 C 0 to 150 C 505 x 300 x 150 mm 330 TIA 540 330 x 395 mm 5726 261 385 x 300 mm stainless steel 7 5 kg 125 180 mm T100 ST26 B mm aan 505 x 300 x 200 mm 0 to 100 C ST38 38L 575 x 300 mm stainless steel 11 kg 125 180 mm T100 S38 690 x 300 x 200 mm 0 to 100 C W 330 mm 730 x 333 x 405 mm P5 5 L plastic 2 5 e 120 x 150 mm hj Ae 9 85 140 mm T100 P5 TC120 P5 TX150 P5 ees e d 240 mm 240 x 160 150 mm amb 15 to 99 C amb 15 to 99 amb 15 99 C 99 w 330 mm 390 x 200 x 360 mm P12 12Lplastic 35kg 210x 280 mm h 180 mm 85 140 mm T100 P12 TC120 P12 TX150 P12 TXF200 P12 d 415 mm 325 280 150 mm amb 5 to 99 amb 5 to 99 C amb 5 to 99 C amb 5 99 C w 350 mm 415 x 350 360 mm P18 18L plastic 5 kg 280 x 325 mm h 180 mm e 85 140 mm T100 P18 TC120 P18 TX150 P18 TXF200 P18 d 600 mm 510 x 290 x 150 mm amb 5 to 99 C amb 5 to 99 C amb 5 to 99 C amb 5 99 C w 365 mm 600 x 350
103. mers and block the reproduction of bacteria as a result causing their death 4 UVR Mi The diagram below shows the process of formation Ag of pyrimidine dimers using thymine as an example source http www photobiology info UVR M TT oa I cH Il N e Destruction of microorganisms using UV radiation The UV intensity needed for the elimination of microor ganisms such as yeasts bacteria and viruses was previously investigated and reported by UVP Inc A table below shows an amount of germicidal shortwave 254 nm UV energy needed for complete destruction of certain microorganisms Table 1 Destruction chart of bacteria and various organisms source http www uvp com pdf ab 115 pdf Bacteria organisms Energy Other Energy mW seconds per cm microorganisms mW seconds per cm Bacillus anthracis 8 7 YEAST S enteritidis 7 6 Saccharomyces ellipsoideus 13 2 Megatherium sp veg 2 5 Saccharomyces sp 17 6 B Megatherium sp spores 5 2 Saccharomyces cerevisiae 13 2 parathyphosus 6 1 Brewer s yeast 6 6 B subtilis 11 0 Baker s yeast 8 8 B subtilis spores 22 0 Common yeast cake 15 2 List continues on the next page List continues on the next page Bacteria organisms Energy mW seconds per cm List continued from the previous page Clostridium tetani 22 0 Corynebacterium diphtheriae 6 5 Eberthella typosa 4 1 Escherichia coli 6 6 Micrococcus cadidus 12 3 Microco
104. mm T100 R5 d 575 mm A7 k w 415 mm 9 Cat num R5 Options and accessories Labwise PC software optional Allows two way communication for status display programming and data capture see p 16 1 for more information External probes optional TXPEP flexible plastic probe 3 m cable TXSEP stainless steel probe 3 m cable Remote switching device optional For switching appliances on and off up to max 8 Amps Vertical turbine pumps optional Low noise compact design Supplied with pipe connections and special lid for fitting to tank pipe bore 12 7 mm VTP 1 n max pressure 1 000 mbar max flow 9 L min VTP 2 i max pressure 1 650 mbar max flow 12 L min TC120 h 315 mm d 145 mm w 115 mm TC120 R1 TC120 R2 TC120 R4 TC120 R5 TX150 h 315 mm d 145 mm w 115 mm TX150 R1 TX150 R2 TX150 R3 TX150 R4 TX150 R5 TXF200 315 d 145mm w 115 mm TXF200 R1 TXF200 R2 TXF200 R3 TXF200 R4 TXF200 R5 Required only where application demands a higher pressure than that delivered by the internal pump to maintain flow MAGNETIC STIRRERS OVERHEAD STIRRER DESCRIPTION Magnetic Stirrers Overhead Stirrer MS 3000 and MMS 3000 Magnetic Stirrers MS 3000 and MMS 3000 are compact magnetic stirrers with stainless steel working surface Units provide stirring of liquids with rotation speed of magnetic element up to 3000 rpm Up to date it
105. n External power Input AC 100 240 V 50 60 Hz supply Output DC 24V ORDERING INFORMATION Cat number Multi RS 60 BS 010118 AAI with standard platform PRS 48 Optional platforms PRS 8 22 BS 010118 AK PRS 14 BS 010118 BK NOILdId SAC P SNOILV2IdIO3dS P Devices Rockers Shakers Rotators Vortexes Platforms for Multi Bio RS 24 Standard Capacity Tube Volume Tube Diameter Cat number Ores 26 1 5 15 ml 10 16 mm BS 010117 GK LC ma Sek rcm SSH Platform for microplates and racks for tall tubes 0 5 and 1 ml e g Thermo prs 1pP 3741MTX 3742MTX 3744MTX eo ion prs 26 prs 5 12 s prsc 22 Clamps on PRSC 10 PRS series platforms are equipped with universal rubber clamps for different size tube fixation PRSC series platforms have metal clamps able to hold heavier solutions e g soil sand Platforms for Multi RS 60 Standard Capacity Tube Volume Tube Diameter Cat number PRS 48 48 1 5 15 ml 10 16 mm BS 010118 CK Optional e PRS 8 22 8 and 22 up to 50 and 1 5 15 ml 20 30 and 10 16 mm BS 010118 AK 14 14 up to 50 20 30 BS 010118 BK Prs 48 992 prs 14 Vortex Vortex W 1 plus T cs biosan Devices Rockers Shakers Rotators Vortexes V 1 plus Vortex Vortex V 1 plus is an ideal instrument for gentle mixing to vigorous resuspending
106. n PCR Cabinets Protection against contamination forming units Viruses DNA RNA Amplicons Table 5 Relation of risk groups to biosafety levels practices and equipment source Laboratory biosafety manual Third edition Risk Biosafety Laboratory Laboratory Safety Group Level Type Practices Equipment 1 Basic Biosafety Basic teaching research GMT None open bench work Level 1 2 Basic Biosafety Primary health services GMT plus protective clothing Open bench plus BSC for Level 2 diagnostic services biohazard sign potential aerosols research 3 Containment Special diagnostic As Level 2 plus special BSC and or other primary Biosafety Level 3 services research clothing controlled access devices for all activities directional airflow 4 Maximum Contain Dangerous pathogen As Level 3 plus airlock entry Class Ill BSC or positive pressure ment Biosafety units Level 4 disposal shower exit special waste suits in conjunction with Class Il BSCs double ended autoclave through the wall filtered air BSC biological safety cabinet GMT good microbiological techniques 122 Applications Articles Table 6 Summary of biosafety level requirements source Laboratory biosafety manual Third edition Biosafety Level 1 2 3 4 Isolation of laboratory No No Yes Yes Room sealable for decontamination No No Yes Yes Ventilation nward airflow No Desirable Yes Yes Controlled ventilati
107. n types Description Q gt orbital Simple orbital motion with an option of shifting direction clockwise anticlockwise after set time lt gt Reciprocal Orbital motion with shifting direction of rotation ah Vibrating High speed low amplitude motion for switching to the next motion in the cycle Description and pictures of all platforms can be found on page 15 Shaker PSU 20i provides three motion types orbital reciprocal and vibrating which can be performed separately pairwise and sequentially in repeated cycles Shaker is designed for applications both in small specialized laboratories and in large multidisci plinary laboratories PSU 20i is an ideal instrument for laboratories conducting research in biopharmaceutics and biomedicine Shaker PSU 20i is noiseless and reliable in opera tion incorporates a direct drive system and brushless motor with a guaranteed service life up to 35 000 working hours The use of direct drive and brushless motor allows for continuous mixing up to 7 days and ensures reliable operation for more than 2 years A choice of nine 9 different interchangeable plat forms provides possibility of performing various procedures and techniques Special attention should be paid to a multilevel platform which allows accom modation of a large number of various microplates Petri dishes cultural bags and other low containers Shaker can be used in cold rooms or incubators oper
108. ng during time gap between two work cycles Possibility to use microtest plates by different manufac turers ensured by automated plate set up adjusting to different depths of plate wells Round bottom plate and strip washing mode Possibility of user defined programs with adjustable parameters ORDERING INFORMATION Cat number 3D IW8 BS 060102 AAI 4 CHW Logger BS 060102 AK INTELIWASHER 3DW amp The unit has 50 programs divided into 5 following aspiration categories see figure bellow Q Type 1 1 0 1 9 IPF96 U V is intended for round and V shape immunoplates 1 point aspiration Type 2 2 0 2 9 IPF96 FLAT 2 is intended for flat bottom shape immunoplates 2 point aspiration Type 3 3 0 3 9 IPF96 FLAT C is intended for rectangular shape immunoplates full circle aspiration direction Type 4 4 0 4 9 FastFRAME 2 is intended for multi slide plate with rectangular wells Type 5 5 0 5 9 FastFRAME C is intended for multi slide plate with rectangular wells The FastFRAME Schleicher amp Shuel multi slide plate or analog plate of another manu facturer that is compatible with standard 25x76 mm 1 x3 inch glass slides 000000060 OOOOOO0O0 OOOOOOOO 00000000 Washers Aspirator 3D IW8 Inteliwasher Minimum dispense volume 25 ul Maximum dispense volume 1 600 ul Dispense increment 25 ul Dispensing accuracy 2 5 Allowed
109. ng system No Desirable Yes Yes HEPA filtered air exhaust No No Yes No Yes Double door entry No No Yes Yes Airlock No No No Yes Airlock with shower No No No Yes Anteroom No No Yes Anteroom with shower No No Yes No No Effluent treatment No No Yes No Yes Autoclave Onsite No Desirable Yes Yes In laboratory room No No Desirable Yes Double ended No No Desirable Yes Biological safety cabinets No Desirable Yes Yes Personnel safety monitoring capability No No Desirable Yes a Environmental and functional isolation from general traffic b Dependent on location of exhaust see Chapter 4 of Laboratory Biosafety Manual c Dependent on agent s used in the laboratory d For example window closed circuit television two way communication Acknowledgement We acknowledge BioSan for financial support and technical assistance Anete Dudele for work done in the beginning of the research on microbial contamination in PCR cabinets We acknowledge Central Researcha Institute of Epidemiology Moscow Russia and M Markelov G Pokrovsky and V Dedkov in particular for development and provision reagents for lambda DNA quantitative analysis using Real Time PCR method We acknowledge Paul Pergande for donating his time and expertise by reviewing this article References KLinden A Mofidi 2004 Disinfection Efficiency and Dose Measurement of Polychromatic UV Light 1 6 BioSan UV air flow Cleaner Recir
110. ng the sequence of three motion types Multi Rotation option of Biosan instruments substan tially expands possibilities and enhances efficiency of sample preparation for the examined materials and allows adjusting the mixing procedure according to the individual tasks Programmable Rotator can be used for variety of applications in modern life science laboratories for hybridization reactions cell growing soft extraction and homogenisation of biological components in solutions as well as for reactions of binding and washing of magnetic particles Programmable Rotator can be used in cold rooms or incuba tors operating at ambient temperature range 4 C to 40 C Low voltage external power supply 12 V provides electrical safety in humid environment vertical rotation Speed control range Vertical rotation movement Time setting range 1 100 rpm increment 1 rpm overhead 360 0 250 sec 2 Reciprocal rotation Speed control range Tilt angle range Time setting range vibro rotation Tilt angle range Pause Vibro time setting range General specifications Digital time setting 1 100 rpm increment 1 rpm 1 90 increment 1 0 250 sec 0 5 increment 1 0 5 sec 1 min 24 hrs non stop increment 1 min Maximum load 0 5 kg Overall dimensions W x D x H 365x195x155 mm Weight 1 7 kg Input current power 12V 660 mA 8W consumption External power supply Input AC 100 240 V 50 60 Hz Output DC
111. ocessor thermocontroller provides a constant temperature control in the incubator chamber CC Forced heated air circulation inside the transparent U plexiglas chamber guarantees even temperature distribu B tion Dismountable construction makes transportation C easy Orbital shaking is controlled by the digital tachometer rpm and Digital time setting regardless of the tempera ture The unit is equipped with the direct drive system ensuring most reliable stable long time operation up to 30 day nights The ES 20 is extremely easy to operate with very straight forward setup of temperature speed and time using the two line set up and status display which clearly indicates both set and actual values for each of the three parameters a Temperature setting range 25 C 42 C Y Temperature control 5 C above ambient 42 C range Q Setting resolution 0 1 C 1 rpm al Product video is available on the website D Temperature stability 0 5 C TE Speed control range 50 250 rpm HEAT UP TIME FOR ES 20 Orbit 10 mm LLI Digital time setting 1 min 96 hrs non stop increment 1 min ES 20 with platform P 6 250 Plexiglas walls thickness 7 mm Load capacity 2 5 kg Overall dimensions WxDxH 340 x 340 435 mm Dimensions of the inner chamber 305 x 260 x 250 mm Weight 13 2 kg Voltage 230 V 50 60 Hz or 120 V 50 60 Hz Current power consumption 160 W 0 7 A 230 120 V 170 W 1 6 A Five interchangeable platforms allow using the ES
112. of Grant Instruments products in Russia CIS and the ORDERING INFORMATION Cat number 9 Baltic States Latvia Lithuania Estonia and an official distributor for a number of other regions OLS26 OLS26 Description and pictures of all available accessories can be found on page 65 Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators Linear shaking bath LSB Aqua Pro range World renowned shaking water baths High quality robust design with unique magnetically coupled shaking mechanism for maximum reliability consistency and quiet operation Extensive range of accessories to provide the right solution for your application Varied vessels types can be securely held using high quality springs clamps or racks FEATURES e Ambient 5 C to 99 C operation Stability 0 1 C Choice of two models 12 and 18 litre e Drain tap for convenient emptying e 3 year warranty e Polycarbonate lid included e Extensive choice of accessory shaking trays Tray sold seperately DESCRIPTION Linear shaking bath LSB12 LSB18 9 2 kg 11 2 kg h 275 mm h 275 mm d 360 mm d 565 mm w 335 mm w 335 mm SPECIFICATIONS Tank size 12L 18L Minimum working depth 60 mm Temperature range ambient 5 to 99 C Uniformity DIN 12876 3 0 1 C 70 C Stability DIN 12876 3 0 1 C 70 C Display LED
113. of the cabinet is surrounded by negative pressure preventing the interaction of the product and the operator 3 PHYSICAL INACTIVATION OF THE DNA RNA MOLECULES IN THE AIRFLOW 2 THERMOSTATED AIRFLOW TLC S is equipped with a UV air recirculation system 4 UV lamps mounted behind the rear wall of the cabinet continuously disinfect the airflow UV air recirculators as has been shown previously by BioSan 1 are highly effective in the inactivation of infectious biological materials which size is much smaller than the pores of HEPA filter less than 0 3 micron such as viruses DNA RNA fragments and amplicons Due to the recirculation of the preheated air we guarantee not only thermostated working surface but also uniform temperature distri bution within the entire working area of the cabinet As a result the instruments shakers rockers centrifuge vortex materials pipetts pipette tips racks reagents kits for cultivation and differentiation of cells culture media and cell lines or inoculum all of the above is at the Eo quantification MARKET N 4 Ek p S 4 j VP gt E7 Molecular biology Cell biology Stem cells technology TLC S Thermostated Laminar Flow Cabinet PROTECTION OF THE PRODUCT THE OPERATOR AND ENVIRONMENT TTL S moving table for the cabinet INFORMATION ABOUT THE AVAILABILITY OF THE DEVICE
114. oling plug in blocks and the right part for heating plug in blocks Both of them are regulated independently and can realize up to 16 programs including temperature and time in each program Peltier technology is used for cooling below room temperature PCB is used for heating till 150 C Separation of cooling and heating parts from each other increases durability of the instrument and speed of tempera ture changing after setting a new program Heating Block Specifications Temperature setting range 25 C 150 C Temperature control range 5 C above ambient 150 C Setting resolution 1 C Stability Cooling Block Specifications Temperature setting range 3 C 20 C Temperature 23 C below ambient control range 5 C below ambient Setting resolution 0 1 C Stability EOC General Specifications 1 min 99 hrs 59 min increment 1 min Digital timer with sound alarm User adjustable programs 16 heating temperature and time 16 cooling Display LCD Overall dimensions WxDxH 295 285 220 mm Weight more 5 6 kg Heating power 500 W Nominal operating voltage 230 50 60 Hz Power consumption 420 W 1 8 A 810 13 Q 85 29 818 12 NOILdId SAC D 5 1 21412345 P DESCRIPTION SPECIFICATIONS Thermostated equipment Thermostats Block Heating Cooling systems QB Series Dry Block Heating Systems with Interchangeable Blocks Equipment present
115. on Turbidity is determined in McFarland units Photometers DEN 1 and DEN 1B McFarland Densitometers Display resolution 0 1 McF DEN 1 DEN 1B Light source LED Wavelength 565 15 nm Measurement range 0 3 15 0 McF 0 00 15 00 McF 0 01 McF Accuracy of the full scale 3 Measurement time 1 sec Sample volume not less than 2 ml Tube external diameter Possibility to restore factory calibration settings Display Overall dimensions Wx D x 18 mm without adapter or 16 mm using optional A 16 adapter NEW EUNCTION 165x115x75 mm Weight 0 7 kg Independent power supply 3 x AA batteries Input current power consumption 12V 80 mA 1 W 12 V 7 mA 0 1W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V Input AC 100 240 V 50 60 Hz Output DC 12V Standard set External power supply External power supply or 3 x AA batteries ORDERING INFORMATION DEN 1 Cat number BS 050102 AAF DEN 1B BS 050104 AAF Optional accessories CKG16 CKG18 BS 050102 BK for glass tubes with diameter 16 mm BS 050102 CK for glass tubes with diameter 18 mm A 16 adapter BS 050102 AK DEN 1B Alc idena eres eine DEN 1B McFarland Daensitametear _ A oum 5 DEM 1H MF Units DESCRIPTION SPECIFICATIONS Photometers HiPo MPP 96 Microplate Photomete
116. on request ALA 1 4 on request Electrophoresis Systems on request Real time Amplificator Product Line Examples Sample Preparation temperature TLC S page 92 controlled laminar flow cabinet Cultivation ES 20 36 or Results Reading DEN 1B or DEN 1 page 96 i Cultivation and Real time RTS 1 RTS 1C page 38 Results Logging t v Gm ZR C1 Sample preparation in UVC T M AR page 86 UV Cabinet for PCR or similar see PCR cabinets page 87 Sample and Reagents MPS 1 page 16 war or similar Resuspending see Vortexes page 23 High Speed Centrifugation Microspin 12 page 43 Vacuum Aspiration FTA 1 page 104 NOILOVu IX3 VNH VNQ ADOTONHO3LIB NOILWAILIND 1130 TWISOYDIW ADOTONHDIALD Incubation TDB 120 page 55 130 Product Line Examples ak St Ow j XU 4 pet 4 i A Ti Ne 3 a nA 4 te TECHNOLOGY ELISA FOR THE DETECTION OF HBS ANTIGEN oy TSS controlled laminar flow cabinet LA rd ED r TN Sample Preparation in temperature TLC S page 92 Incubation PST 60 HL page 28 Washing Automated IW 8 page 100 Washing Manual FTA 1 with MA 8 page 104 Analysis
117. operation time 168 hrs eo Direct drive mechanism ua Product video is available on the website Digital time setting 1 min 24 hrs non stop Non slip silicone mat is supplied as standard Maximum load 1 kg Display LED PDM dimpled mat Platform working area 215 215 mm Overall dimensions W x D x H 220 x 205 x 120 mm Weight 2 1 kg Input current power consumption 12V 320 3 8 W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V ACCESSORIES FOR THE STANDARD PLATFORM Optional dimpled mat PDM prevents different size tubes from rolling around the platform ORDERING INFORMATION Cat number MR 1 with standard platform Bio PP 4S 5 010152 Optional accessories PDM dimpled mat PDM MR 1 with PDM dimpled mat MR 1 with Petri dishes e Devices Rockers Shakers Rotators Vortexes MR 12 Rocker Shaker 1 t A Rocking uni rotation with adjustable tilt biofan MR 12 Rocker Shaker provides both soft and intensive mixing of solu tions or nutrient media in vessels or plastic bags placed on the platform Adjustable speed and platform tilt angle allows setting parameters for optimal solution transfer and mixing The device is ideal for gel destaining after electrophoresis and homogeni sation of bioextraction media It is optimal for biomolecule hybridization on strips and for staining destaining procedures When installed inside a bioincubator it is idea
118. ories and industrial biotechnological laboratories Micro Spin provides simultaneous mixing and separation of samples using centrifuge and mixing modules located on the puc ROSIN aa common spin module PN gm de FV 2400 is an open type centrifuge without lid that increases the speed of centrifugation and resuspension operations Rotation speed fixed 2 800 rom 3 500 rom Max RCF 450xg 700xg Continuous and impulse operation modes Overall dimensions WxDxH 120x170x120 mm Weight 1 4kg Nominal operating voltage 120 or 230 V 50 Hz 120 or 230 V 60 Hz Power consumption 120 230 V 30 W 0 27 A 30 W 0 13 A 30 W 0 27 A 25 W 0 1 A SPECIFICATIONS T ORDERING INFORMATION Cat number FV 2400 white BS 010201 AAA with standard rotor R 1 5M and R 0 5 0 2M Optional rotors see table below Optional rotors Capacity Type Cat Number R 0 5 0 2M 12 x0 5 ml and 12x0 2 ml microtubes 24 Standard BS 010201 BK R 1 5M 12x 1 5 ml microtubes 12 Standard BS 010201 AK R 2 0 5 8 x 2 0 ml and 8 x 0 5 ml microtubes 16 Optional BS 010205 CK n 2 0 5 0 2 6 2 0 ml 6 0 5 ml and 6x0 2 ml microtubes 18 Optional BS 010205 DK sR 16 Two 8 section strips for 0 2 ml microtubes 16 Optional BS 010202 AK sR 64 Eight 8 section strips for 0 2 ml microtubes 64 Optional BS 010201 EK for any type of stri
119. ors needed for more powerful cooling requirements e g 50 to 50 C Silicone oil low viscosity the removal of mechanical or electrical heat produced in Bayer silicone M3 apparatus or machinery Please contact Grant for advice 30to 30 C 50 water 50 antifreeze inhibited ethylene glycol Oto 30 C 80 water 20 antifreeze inhibited Model selection see page 74 ethylene glycol The four Grant Optima thermostats can be combined 5 to 99 9 Water with the five Grant refrigeration units to provide a choice of 18 models Heating Circulators Specifications and all available accessories on page 74 Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heatina cooling Circulators Optima series Refrigerated Thermostatic Baths Combinations and Accessories Capacity L Working area L x W T100 Outer tank dimensions Min max liquid depths h 315 mm e Weight d 145 mm w 115 mm R1 5L stainless steel h 410 mm e 110 145 mm 4 410 mm e 80 140 mm T100 R1 w 230 mm 20 kg Cat num R1 R2 5L stainless steel h 410 mm 110 145 mm d 410 mm e 80 140 mm T100 R2 w 230 mm e 20 kg Cat num R2 R3 5 L stainless steel h 410 mm e 110 145 mm 410 mm 80 140 mm w 230 mm 21 kg Cat num R3 R4 20 L stainless 230 x 305 mm e 80 140 mm T100 R4 d 490 mm 40 kg w 390 mm Cat num R4 R5 12L stainless oe vam 260 115 mm 120 180
120. ortex 3 mixing modes soft medium hard regulated time Vortexing regulation timer 1 20 sec ETA 9 et Multi Spin msc 6000 3 Centrifuge Vortex 4 SMS cycler for realization of the sms algorithm MSC 3000 MSC 3000 Multi Spin Both product video is available on the website SAVING TIME WITH MULTI SPIN E cag Multi Spin allows considerable time saving ama 9 compared to Combi Spin by automatically b d Iss rformin ling program of sample mixin 7 ee e nie i spin cycle for 12 microtubes simultaneously FVL 2400N MSC 3000 MSC 6000 Speed control range max 2 800 rom 3 500 rom 6 000 rpm RCF max 700xg 800xg 2350xg Number of tubes vortexing 1 individually 12 simultaneously Time for completing spin mix spin cycle for 2 microtubes 60 sec 25 sec 15 sec for 12 microtubes 5 6 min 90sec 60 sec for 100 microtubes 60 min 15 min 10 min Unit price ratio 15x 00 16x Minicentrifuges Vortexes Mini centrifuge Centrifuges Specifications Speed regulation range increment 100 rpm RCF max Spin timer Vortexing intensity Vortexing time SMS cycle regulation Display Safety Overall dimensions W x D x H Weight Input current power consumption External power supply motor R 1 5 MSC 3000 and MSC 6000 Mu
121. osan is the only company in the World of Biotechnomica which develops produces and distributes instru ment lines for all 4 levels of diagnostics These Vasily Bankovsky Ph D Biology Head of R amp D Department Chairman of the Board at Biosan satellites of 4 planets are specialised devices providing the instrumental basis for multilevel diagnostics whereas the reagent sets make these satellites move On this account the term Biotech nomica in our understanding means the branch of biotechnology responsible for the development of multilevel laboratory diagnostics sets instrument lines In the future perspective multidiagnostic chips may appear with the development of chip technologies allowing to unify all the aforesaid technologies in one chip Biosan plans to be active in this field in the next few years am pleased to point out that many of our ideas and products have been developed as a result of long standing cooperation of scientists from the Institute of Microbiology of the Latvian Academy of Science where our company was founded 23 years ago and where it is presently located with universities as well as with academic institutes and institutes of applied sciences and our company customers worldwide All our inventions resulted from joint efforts and today we are still open for collaboration We will be delighted if the result of our work which has already received wide recognition of the Western sc
122. preparation market The Spin Mix Spin technology is intended to spin down micro volumes of reagents on the well s bottom first centrifugation spin following mixing mix and spin down the reagents again from the walls and cap of the well second spin We named this repetitive algorithm of operation that is aimed at reducing the mistakes during sample preparation for PCR analysis sms algorithm This algorithm is registered by BioSan DESCRIPTION CVP 2 is a fully automatic device for reproducing sms algorithm for 2 PCR plates at the same time thus saving time considerably A must have instru ment for PCR and DNA analyses laboratory CVP 2 is 4 devices combined in 1 1 Centrifuge Maximum RCF 245 x g 1 500 rpm 2 Vortex 300 1 200 rpm Vortexing regulation timer 0 60 sec 3 Centrifuge Vortex 4 SMS cycler for realization of the sms algorithm Speed regulation range Max RCF 300 1 500 rpm 245 xg Vortex regulation range 300 1 200 rpm Setting resolution 100 rpm Plate type e Without adapter 96 well skirted PCR plates PCR strips in a frame 96 well semi skirted and non skirted PCR plates e With adapter AP 384 384 well PCR plates Display LCD 64 x 128 pixels 0 30 min e With adapter AP 96 SPECIFICATIONS Centrifugation mode time range Centrifugation mode time increment 1 s after 1 min 1 min Vortex mode time range 0 60 sec Number of programmable cycles 1 999 Chamber diamet
123. ps including paired R 0 5 0 2M Tube vortexing on FV 2400 Minicentrifuges Vortexes Mini centrifuge Centrifuges Combi Spin FVL 2400N Product video is available on the website Optional rotors Capacity Type Cat Number o R 0 5 0 2 12 x0 5 ml and 12x0 2 ml microtubes 24 Standard BS 010205 BK e R 1 5 12 1 5 ml microtubes mE 12 Standard BS 010205 AK e R 2 0 5 8x2 0 iil and 8x 0 5 al neues 16 Optional BS 010205 CK o R 2 0 5 0 2 6x2 0 ml 6x0 5 ml and 6x 0 2 ml microtubes 18 Optional BS 010205 DK SR 16 Two amp sectionstripsfor0 2 ml microtubes 16 Optional BS 010202 AK SR 32 Four 8 section strips for 0 2 ml microtubes 32 Optional BS 010205 FK FVL 2400N Combi Spin Mini centrifuge vortex Combi Spin FVL 2400N is specially designed for genetic engineering research for PCR diagnostics experiments Units can be used in microbiological biochemical clinical laboratories and industrial biotechnological laboratories Combi Spin provides simultaneous mixing and separation of samples using centrifuge and mixing modules located on the common spin module FVL 2400N is provided with protection mechanism that stops the rotor motion when the lid is opened Rotation speed fixed 2 800 rpm 3 500 rpm Max RCF 450xg 700xg Continuous and impulse operation modes Safety Stop at open lid Overall dimensions WxDxH 190x235x125 m
124. r Microplate Photometer Hipo is a compact tabletop de vice for measuring the results of ELISA and microbio logical studies in 96 well microplates Photometer is con trolled outputs data via computer The device is supplied with specialized software QuantAssay Features of QuantAssay software e ELISa assays of any complexity can be carried out via robust assay editor e Multiplex assays up to 7 assays on one plate e Qualitative assay includes up to 8 controls e Quantitative assay includes up to 20 standards e BestFit function for selecting the best calibration curve Save load and export results e Creating visual reports Detection mode Absorbance Light source LED self calibrating Photodetector 8 silicon photodiodes 96 well microplates including strip well microplates Plate type Reading Speed 5 s per wavelength Measurement modes Endpoint kinetic and multi label measurements Measurement channels 8 Reference channel 1 Measurement range 0 4 3 OD Resolution 0 0001 OD Wavelength range 400 750 nm Wavelength selection up to 8 filters on wheel standard filters 405 450 492 and 620 nm Shaking 4 amplitudes 4 speeds Software QuantAssay PC system Intel AMD Processor 1 GB RAM Windows Vista 7 8 2 0 USB 140 x 300 x 130 mm requirements Overall dimensions WxDxH External power supply Input AC 100 240 V 50 60 Hz Output DC 12V up to 4 custom filters can be fitted on demand ORDER
125. r washing standard 96 well plates during analyses The unit provides Washing mode Rinsing mode Mixing mode Double aspiration Possibility of additional solution mixing during time gap between two work cycles Possibility to use microtest plates by different manufacturers ensured by automated plate set up adjusting to different depths of plate wells Plate and strip washing mode User defined programs with adjustable parameters Saving work programs IW 8 BS 060106 AAI 4 CHW Logger BS 060102 AK Washers Aspirator IW 8 Intelispeed Washer p 1 perasot protection lid Choice of 3 washing liquid bottles Minimum dispense volume 25 ul Maximum dispense volume 1 600 ul AA Dispense increment 25ul Dispensing accuracy 20500 a Allowed residual liquid volume no more than 2 ul in plate well Number of wells washed simultaneously 8 O Number of washing cycles for each channel 1 15 Aspiration time 0 2 3 sec Aspiration dispensing speed 3 levels Max number of channels in a program 2 Soaking time 0 300 sec increment 10 sec Shaking time 0 150 increment 5 sec Number of washed rows 1 12 Time of plate single wash 350 ul no more 45 sec 4 channel washing solution weight logger Number of programs 100 4 CHW Logger provides automatic control of rinsing solution and waste volumes The washer Plate platform and washing head movement automated shows remaining volume for each bottle as percentage
126. rainer TS Sake dispense valve enc c mp io Polishing A Final point Reagent preparation es es module L TE lonch romatography yes yes ICP MS yes yes 19 Ultrapure water qualit L mol sensor Atomic absorption spectro dispense filter h 8 h hi Storage pedi p otometry Wit grap ite yes yes tank module 185 254 nm ato m ize r ure Grade ICP OES es es WERDEN AT Labaqua HPLC y y HPLC yes yes Pure water Co 0 Nes Gas chromatography yes yes reverse I Bete Total Organic Carbon TARE module 7 measurement presure RO module Recirculation Polishing EISE E oF use filter Flow cytometry N A yes UV sterilization Inpu Checkvalve module f vale Flow 254 nm Eo Cell and tissue culture N A yes Molecular biology N A yes Activated carbon 5um pre filter Ultrapure water quality sensor ure water Protective dispense filter Dual wavelength Storage UV photooxidation tank module 185 254 nm Pure Grade 2 water dispense pump Labaqua Bio SPECIFICATIONS ULTRAPURE WATER SYSTEMS Labaqua series The Labaqua has important safety functions e Tank filling control e Tap water pressure control Protection from tank sensor failure The Labaqua systems include e Boost pump e Pre filter set Reverse osmosis module e Deionization module e Final stage polishing module 25L storage tank with an int
127. residual liquid volume in plate well not more 2 ul Number of wells washed simultaneously 8 Number of washing cycles 1 15 Aspiration time 1 3 sec Final aspiration time 1 3 sec Aspiration dispensing speed 3 levels Max number of channels in a program 2 Choice of 3 washing liquid bottles Soaking time 0 300 sec increment 10 sec Shaking time 0 150 sec increment 5 sec Number of washed rows 1 12 Time of one plate wash 300 ul not more 45 sec Number of programs 50 Plate platform and washing head movement automated Indication of operation modes LCD 8 line Dimensions WxDxH 375 x 345 x 180 mm Weight with accessories 9kg External power supply DC 12V 4 16A Consumed power not more 60W The unit is designed for use in closed laboratory rooms at temperatures from 10 C to 35 C and relative humidity up to 8096 at 25 C decreasing linearly to 5096 relative humidity at 35 4 channel washing solution weight logger 4 CHW Logger provides automatic control of rinsing solution and waste volumes The washer shows remaining volume for each bottle as percentage and gives a warning message in case of low solution volume or full waste bottle when 4 CHW Logger is connected 4 CHW LOGGER SPECIFICATIONS Max loading per scale cup 2 kg Dimensions 267 x 252 x 97 mm Weight 3 kg 5 1 214132345 P DESCRIPTION SPECIFICATIONS Washers Aspirator FTA 1 Aspirator with Trap Flask Suction hydrophobic m
128. riment was considered successful only if control was negative After samples were taken and extracted as mentioned above qPCR reaction master mix was prepared by adding the following components for each 25 ul of reaction mix to a tube at room temperature PCR mix Sample taking path 2 FL 7 ul dNTP s 2 5 ul Forward Primer 1 ul AT m ee Reverse Primer 1 ul DNA probe 1 ul Template DNA 10 ul A es Water nuclease free to 25 ul Total volume 25 ul p a E Table 1 Cycling protocol a EE EM Three step cycling protocol steps Temperature C Time Number of cycles Initial denaturation 95 5 min 1 Denaturation 95 5 sec 42 Annealing 60 20 sec 42 Extension 72 15 sec 42 Detection Channel FAM 118 Applications Articles O Air Surface samples Fig 4 DNA extraction samples analyses and result detection DNA extraction From Air Samples Incubation on Shaker OS 20 BioSan 180 rpm 15 Spin columned GeneJet Plasmid Miniprep Kit Thermo Fisher Fermentas Q From Surface Samples e Vortex 2 3 e Centrifuge at 13 300 rpm for 2 Isolated DNA Real time PCR amplification Fig 7 O Detection of Ct values and normalization of data Fig 8 Copy number estimation on cabinet volume and surface area O5 Results Mechanical contamination Microbial contamination Results o
129. rotection lid MSL SC and adapters A 02 A 05 Additional replacement parts MSL SC protection lid for rotors BS 010213 EK A 02 12 pieces for microtubes 0 2 ml BS 010213 AK O A 05 12 pieces for microtubes 0 5 ml BS 010213 BK NOlILdld5Ss3CG D 5 1 21412345 P Minicentrifuges Vortexes Mini centrifuge Centrifuges LMC 3000 Laboratory Centrifuge amp l LMC 3000 is a modern low speed bench top centrifuge designed for operation with microtest plates and centrifuge tubes up to 50 ml This device is widely used in biomedical O profile laboratories Q FEATURES QC Soft start and run down of the rotor e User friendly setting of centrifugation parameters speed LLI and time and simultaneous display of both set and actual C values e Safe operation at any speed is provided by metal protection chamber and case cover automatic stop at imbalance and a lock keeping the lid closed while the centrifuge is running e Low noise level Possibility to switch off forced braking NEW FUNCTION e Wide choice of accessory rotors see page 50 Speed regulation 100 3 000 rpm U range for centrifuge tubes 1 700 x g Speed regulation 100 2 000 rpm range for microtitre plates 560 x g lt Setting resolution 100 rpm Rotor imbalance diagnostics O automatic stop IMBALANCE warning Display LCD 2x 16 signs Q uU Digital time setting 1 90 min increment 1 min Chamber diameter 335 mm Overall
130. rs 65 dB 5 1 kg 22550215 x 150 mm 12V 800 mA 10 W Input AC 100 240 V 50 60 Hz Output DC 12 V Deepwell plate 96 500 ul Cat number BS 010216 A03 Optional platforms Cat number P 02 96 For semi unskirted PCR plate or 96 microtest tubes 0 2 ml BS 010216 CK P 2 24 For 24 microtest tubes 1 5 2 ml BS 010216 AK P 05 32 For 32 microtest tubes 0 5 ml BS 010216 BK P 02 05 For 24 microtest tubes 0 5 ml and 48 microtest tubes 0 2 ml BS 010216 DK Platform P 02 96 Platform P 2 24 Platform P 05 32 Oo Platform P 02 05 5 1 21412345 P DESCRIPTION SPECIFICATIONS Devices Rockers Shakers Rotators Vortexes PSU 2T Mini Shaker Mini Shaker PSU 2T is designed for immunoassays and provides adjustable mixing of reagents in micro plates The device ensures smooth movement of the platform even at low speeds Shaker is a compact and user friendly device It takes up little space on a desk and is ideal for personal use The use of direct drive and brushless motor allows continuous mixing up to 7 days and ensures reliable trouble free operation for more than 2 years Display of the device switches between time and speed readings Shaker can be used in cold rooms or incubators oper ating at ambient temperature range 4 C to 40 C Speed control range 150 1 200 rpm Digital time setting 1 min 24 hrs non stop Digital setting and control of time an
131. s in different size and type plastic tubes 0 2 to 50 ml It is designed for operation in life science laboratories working in the fields of biochemistry cell and molecular biology Unit has four types of interchangeable platforms for Eppendorf type microtest tubes 10 15 50 ml tubes diameter 12 16 30 mm Platforms can be ordered separately or as one set with MSV 3500 Speed and time are under microprocessor control LCD display indicates two lines of values the set and actual values of speed and time Unit provides high maximum speed of platform rotation effi ciently mixing microvolumes less than 5 ul of samples 300 3 500 rpm Maximum speed depends on load 0 60 min non stop increment 1 min Speed control range Digital time setting Display LCD 2x 16 signs Orbit 4 mm Maximum load 0 2 kg Maximum continuous operation time 8 hrs Dimensions 180x170x145 mm Weight 2 6 kg Input current power consumption 12V 1A 12W External power supply Input AC 100 240 V 50 60 Hz ORDERING INFORMATION Cat number BS 010210 TAH BS 010210 AAH MSV 3500 with all 4 platforms MSV 3500 without platform Optional platforms see table below Cat Number BS 010210 CK BS 010210 BK BS 010210 DK BS 010210 AK sv 3 15 sv 4 30 NOILdId SAC P 5 1 21412345 P CATALOGUE 2015 2016 THERMO SHAKERS DESCRIPTION um ee Thermo Shakers Shakers Incubators Real Time Cell Growth Logger PST 60HL
132. s which provide Reverse Spin type of agitation and logging of microbial growth in 50 ml tubes in real time e Innovative mixing due to reverse spinning of the sample around its own axis e Due to innovative mixing technology it is possible to measure optical density of the probe in real time e Software has been developed store display and analyse the data in real time RTS 1 and RTS 1C are compact devices with low profile and small footprint for personal application Temperature control allows to use RTS 1 and RTS 1C as an incubator e g for cell growth RTS 1C has a function of active cooling and tem perature profiling via software e The ability to change parameters such as tempera ture RPM and Reverse Spin frequency allows to achieve consistency and reproducibility of results Programmable Cycling profiling of cultivation pa rameters such as temperature RPM Reverse Spin frequency The ability to remotely monitor the process of cultivation while home or using a mobile phone ANNOUNCEMENT TLC S Thermostated Laminar Flow Cabinet INFORMATION ABOUT THE AVAILABILITY OF THE DEVICE WILL BE PUBLISHED ON OUR WEBSITE WWW BIOSAN LV Thermostated Laminar Flow Cabinet TLC S is a laminar flow biological safety cabinet with HEPA filters that is intended for processing of biological materials The device is designed for use in laboratories focused on DNA analysis genetic engineering molecular bio
133. sec Measurements frequency MF is 10 min approx Rotation speed of reactor 2 000 rpm temperature 37 C Diameter of filters pores for aeration 0 25 um Fig 4 Influence of Frequency of Reverse Spinning on the Growth Kinetics vs Time of fermentation of E coli BL21 in a 3D model The Things to Think About Behaviour of Light in the Environment of Different Densities Fig 5 Experiment of Behaviour of Light in the Environment of Different Densities was carried out Green 535 nm laser was used in the Saccharomyces Cerevisiae of different optical densities OD in the range from 1 to 10 with 1 OD increment RTS 1 Reverse Tube Spin cultivation based on the new method of external agitation of cultiva tion media has been shown to be efficient for cultivation of aerobic microorganisms and cell growth logging Effective growth of E coli on LB media has been demonstrated under extremely high speed rota tion of the reactor 2 000 rpm To increase an OD measurement range we inves tigate near infrared IR spectra and showed that a 850 nm wavelength is sufficient to measure increased cell concentrations Such wavelength shift from traditional 600 nm to 850 nm strongly expanded a range of correct OD measurements Moreover we propose a new technology of a non contact high biomass measurements during fermentation based on a formation of a thin layer of cultivation media giving a correct data of
134. tats Block Heating Cooling systems TDB 120 Dry Block Thermostat TDB 120 is a traditional Biosan s dry block thermostat for laboratory analysis Thermostat is designed for maintaining constant temperature of samples in tubes inserted in the aluminium block sockets Unprecedented high precision and uniformity of temperature over the block TDB 120 is widely used for PCR analysis Microprocessor controlled temperature and time Simulta neous indication of set and actual temperature and time Temperature setting range 25 C 120 Temperature control range 5 C above ambient 120 C Temperature setting resolution 0 1 Temperature stability 37 C 0 1 Ud Temperature uniformity 37 C FOIE Display LCD 2 x 16 signs Digital time setting 1 min 96 hrs increment 1 min Overall dimensions WxDxH 230 210 110 mm Weight 2 8 kg Nominal operating voltage 230 V 50 60 Hz or 120 V 50 60 Hz Power consumption 230 V 200 W 870 mA BLOCKS BUILT IN SPECIFICATIONS Two models are available offering a choice of tube config urations to meet the needs of many standard laboratory procedures microtubes from 25 C to 120 C Block A 53 21x0 5 ml 32x 1 5 ml microtubes from 25 C to 37 C ORDERING INFORMATION Cat number TDB 120 with block A 103 BS 010401 QAA TDB 120 with block A 53 BS 010401 PAA Block A 103 Block A 53 21412345 P NOlLdld SAC P DESCRIPTIO
135. terial from 25 C to 4 C TEMPERATURE CALIBRATION FUNCTION With the help of the temperature calibra T tion function the user can calibrate the unit LOPES setn Tange im Bh approx 6 of the selected temperature to Temperature control range 15 C below ambient 100 C WG compensate differences in the thermal behav Temperature setting resolution 0 1 iour of tubes from different manufacturers Temperature stability 40 1 C CY Temperature accuracy 37 C 0 5 C Temperature uniformity over the block 4 C 0 6 C A 37 C 0 1 C D gt TS 100C with block SC 96AC 100 C 0 3 C Average heating speed O from 25 C to 100 C 5 Z Average cooling speed d from 100 C to 25 C 5 C min from 25 C to 4 C 1 8 C min Temperature calibration coefficient range 0 936 1 063 0 063 Speed control range 250 1 400 rpm Acceleration time 3 sec Orbit 2mm Display LCD 16 x2 signs Microprocessor controlled temperature mixing speed and operation time Digital time setting 1 min 96 hrs 1 min increment Maximum continuous operation time max 96 hours Overall dimensions W x Dx H 205 x 230 x 130 mm ORDERING INFORMATION Cat number Weight 3 7 kg TS 100C without block BS 010143 AAI Input current power consumption 12V 4 9 A 60W Catalogue numbers and descriptions of all External power Input AC 100 240 V 50 60 Hz blocks can be found on page 32 supply Output DC 12 V Inter
136. th TOC monitor Optional accessories 0 22 um air vent filter for the storage tank RO membrane for higher capacity 20 L h Electrodeionization module 10 30 L h Water storage tank Comfort w level switch dispense pump 60 L 50 L water storage tank Economy w level switch pump Storage tank Comfort w dispense pump 100L Storage tank Comfort w dispense pump 200L Storage tank Comfort w dispense pump 300L documentation Cat number BS 070101 A02 BS 070102 A02 BS 070103 A02 Cat number BS 070102 AK BS 070102 BK BS 070102 CK BS 070102 EK BS 070102 DK BS 070102 FK BS 070102 GK BS 070102 HK BS 070102 IK Tap water filters for clean tap water with hardness 130ppm CaCO3 Pre filter housing 10 w fittings for clean tap water Sediment filter 1 um for clean tap water BS 070102 JK BS 070102 KK Tap water filters for muddy and or hard tap water with hardness gt 130 ppm CaCO3 Pre filter set 3 x 10 w cartridges polyphos phate 5 um 1 um for muddy hard tap water BS 070102 LK TLC S Thermostated Laminar Flow Cabinet Thermostated Laminar Flow Cabinet TLC S is a laminar flow biological safety cabinet with HEPA filters that is intended for processing of biological materials The device is designed for use in laboratories focused on DNA analysis genetic engineering molecular biology and laboratories working with cell cultures Besides HEP
137. th wheel locks with a drawer are available on request Two versions T 4 for single size UV Cabinets Q T 4L for double size UV Cabinets Other optional furniture is featured on page 105 ADVANTAGES OF BIOSAN UV CLEANER BOXES Ozone free high density UV decontamination Long living UV lamps 8 000 hours average Automatic switch off of UV lamps when the protective screen is opened Bactericidal flow type recirculator providing perma nent decontamination inside UV cleaner box during operation Shockproof glass walls Low noise low energy consumption Tables for installation of UV cleaner boxes UV cleaner boxes with the bactericidal UV cleaner recirculator AR is the patented Biosan solution UVC T M AR UVT B AR on the table T 4 DNA RNA UV Cleaner Boxes Q UVT S AR on the double size table T 4L DNA RNA UV cleaner box UVT S AR with equipment for nucleic acid extraction LF 1 laboratory chest of drawers ORDERING INFORMATION UVC T AR with inlet UVT B AR with internal socket UVT B AR with inlet UVC T M AR with inlet UVC T M AR with internal socket UVT S AR with internal sockets Laboratory Furniture T 4 table T 4L table T 4L P table with shelves LF 1 laboratory chest of drawers m Product video is available 7X onthe website Development and evaluation of DNA amplicon quantification video is available on the website Table T 4 Cat number BS 040102
138. ticles placing enquiries and locating your locally appointed distributor or contact customer service direct at service biosan lv As Biosan is committed to a continuous program of improvement specifications may be changed without notice Applications and Articles THE WORLD OF BIOTECHNOMICA REVERSE SPIN TECHNOLOGY INNOVATIVE PRINCIPLE OF MICROBIAL CULTIVATION DEVELOPMENT AND EVALUATION OF DNA AMPLICON QUANTIFICATION UVR M AND UVR MI UV AIR RECIRCULATORS TEST REPORT HOW TO CHOOSE A PROPER SHAKER ROCKER VORTEX i 128 PRODUCT LINE Applications Articles The World of Biotechnomica In the act of creation man steps beyond himself as a creature and rises above passivity and the coinci dence of his existence into the realm of freedom and meaning In this need to transcend can be found one of the roots not only of love but of art religion and material production Erich Fromm The concept of development for Biosan called The World of Biotechnomica Four planetary systems with satellites devices revolve around Terra Innovatica biomaterial under research We have marked out four planets 4 contemporary diagnostic levels 1 Terra Genomica diagnostics at the level of genes DNA analysis oligonucleotide and mon onucleotide polymorphism ONP SNP 2 Terra Immunologica diagnostics at the level of immunology detection of polymorphism of anti
139. tomati cally restore The graph shows that the cell concen tration range optimal for measurement is 5 30 ml of culture medium in the reactor Fig 2 The effect of Media Volume on it s Layer Thickness mm during Reverse Spin cycle V ml RTS 1 RTS 1 Layer RTS 1 Optical ODonline Thickness Path mm 850nm mm B 2 45 0 55 1 10 2 W 10 1 92 1 10 2 20 15 1 71 1 65 3 30 4 20 1 62 2 20 4 40 5 W 25 1 65 2 25 5 50 6 W 1 45 3 30 6 60 6 7 00 5 6 00 5 00 4 E 4 00 8 3 3 3 00 2 B H 2 00 1 1 100 0 T T 1 T 0 00 5 10 415 20 125 30 Volume ml epe OD 600nm 5 22 pe OD 850nm RTS 1 anymOD 850nm 5 22 pue ayer Thickness mm S 22 ODoff line S 22 ODoff line Cells 850nm 1mm 600nm 1mm cuvette Yield 600 cuvette nm DS 5 17 25 8 2 05 4 51 45 1 1 92 4 22 63 3 1 75 3 85 77 0 1 75 3 85 96 2 1 50 3 30 99 0 OD over time 10 20 36 50 68 7D 80 80 100 110 120 130 140 160 hors Growth rate 10 20 30 40 50 130 140 150 66 70 80 90 100 120 Results obtained indicate that the maximum rate of cell division is detected at a frequency of 1 Reverse Spin per second 1 sec at a speed of 2 000 rom The increase of pause between reverse spins reduces cell growth rate reaching 50 of the maximum value when RS freq 30 sec see fig 3 For a better visual representation of the results the data of three factor interdependence experiments are presented in the
140. ty range nm HEPA filter __0_ 1700 2 300 600 DNA RNA amplicons Viruses Biological agent sizes nm Colony forming units CFU test Media LBA media was prepared using Standard Methods Agar Tryptone Glucose Yeast Extract Becton Dickinson and Company and dissolved in 1 litre of purified water 7 5 grams of Yeast Extract Biolife S r l and 5 grams of Tryptone Difco labora tories were added to enrich the media The media was autoclaved at 121 C for 15 minutes Media control samples were taken to check for presence absence of colony forming units in media itself and the results were negative 0 CFU per 3 plates Air flow organization through HEPA filter HEPA is an acronym for high efficiency particulate absorbing or high efficiency particulate arrestance or as officially defined by the Department of Energy DOE high efficiency particulate air The first HEPA filters were developed in the 1940 s by the USA Atomic Energy Commission to fulfil a an efficient effective way to filter radioactive particu late contaminants HEPA filter technology was declassified after World War 2 and then allowed for commercial and residential use 6 This type of air filter can theoretically remove at least 99 9796 of dust pollen mold bacteria and any airborne particles with a size of 0 3 um at 85 litres per minute l min In some cases HEPA filters can even remove or reduce viral contamination The
141. up to 72 hrs n a Heat up time from 25 C to 100 C 15 min 25 min Power consumption 150 300 600 W 300 W 150 300 W Power supply 115V or 230V All available accessories can be found on page 59 Thermostated equipment Thermostats Block Heating Cooling systems QB Series Dry Block Heating Systems with Interchangeable Blocks Accessories Interchangeable blocks Cat Num QBD1 QBD2 QBD4 QBH2 QBA1 QBA2 No of blocks 1 2 4 2 1 2 QB 0 Plain block without holes i D 3 QB 10 24 x 10 mm holes 50 mm hole depth QB 12 24 x 12 mm holes n ft 50 mm hole depth QB 13 12 x 13 mm holes 50 mm hole depth d P n E Y QB 16 12 x 16 mm holes is n he 50 mm hole depth QB 17H for 10 x Falcon tubes tall n p 17 mm diam 75 mm deep QB 18 12 x 18 mm holes 50 mm hole depth i i D D 24 5 24 holes universal n bottles 50 mm hole depth QB 50 4 x 50 ml centrifuge tubes glass universals 50 mm hole depth QB H 56 x 0 2 ml microtube x 14 mm hole depth QB EO 24 x 0 5 ml microtube n i E D 30 mm hole depth QB E1 24 x 1 5 ml microtube 35 mm hole depth B a T T T QB E2 24 x 2 0 ml microtube 14 mm hole depth ii D i Dolphin nose tube E 24 x 11 13 mm to 6 1 mm External Pt1000 temperature probe QBEP Standard probe For in sample or in block temperature control encased in
142. uracy 37 05 Average heating speed 4 C min from 25 C to 100 C Temperature uniformity over the block 437 6 01 60 0 2 100 2026 Temperature calibration 0 936 1 063 0 063 coefficient range Speed control range 250 1 400 rpm Acceleration time 3 sec Orbit 2mm Microprocessor controlled temperature mixing speed and operation time Display LCD 16 x2 signs Digital time setting 1 min 96 hrs 1 min increment Maximum continuous operation time max 96 hours Overall dimensions WxDxH 205 x 230x130 mm Weight 3 2 kg Input current power consumption 12V 3 5A 42W External power supply Input AC 100 240 V 50 60 Hz Output DC 12V eo un Product video is available on the website eo Mixing Efficiency Video is available on the website HEAT UP TIMES FOR TS 100 from 25 C to 37 C from 37 C to 100 C TEMPERATURE CALIBRATION FUNCTION With the help of the temperature calibration func tion the user can calibrate the unit approx 6 of the selected temperature to compensate differences in the thermal behaviour of tubes from different manufacturers TS 100 with block SC 24 ORDERING INFORMATION Cat number TS 100 without block BS 010120 AAI Catalogue numbers and descriptions of all blocks can be found on page 32 Thermo Shakers Shakers Incubators Real Time Cell Growth Logger TS 100C Thermo Shaker 03mm Thermo Shaker TS 100C provides intensive mi
143. ured in sterile cabinet laboratory air Measurements were performed 9 times and the average value presented in the results as number of particles per m3 of air Two channels were used to measure amount of particles of different size 5 um and 0 3 um Mechanical filter stops particles larger than 5 um while HEPA filter larger then 0 3 um DNA Am pl icon test Fig 3 Air and surface samples and surface sample taking path Instruments e Nebulizer BioSan e Shaker OS 20 BioSan e Mini Centrifuge Vortex FV 2400 BioSan e Centrifuge Pico 17 Thermo Electron Corp e Centrifuge Vortex MSC 6000 BioSan e Real Time PCR cycler Rotor Gene 3000 Corbett Research Reagents e Lambda DNA Thermo Fisher Fermentas uid GeneJet Plasmid Miniprep Kit Thermo Fisher Fermentas Samples taken from Real Time PCR reagents Central Research Institute of 1 2 3 Air Syringes Epidemiology x 4 Working surface Swab X 5 X 7 Side walls Swabs Experiment setup Back wall Swab Sampling was performed as shown on Fig 3 e Extraction and analyses were performed as shown on Fig 4 e Quantitative PCR Polymerase Chain Reaction DNA amplicon quantification in sterile cabinets was performed by qPCR Controls and standards were set in each experiment 4 standards of Lambda DNA of different concentration prepared in 10 fold dilution starting concentration 0 6 ng ul or 1 000 000 copies ul 2 NTC no template control sterile H O expe
144. w voltage external power supply 12 V provides electrical safety in humid environment Speed control range 5 30 rpm Vertical rotation movement overhead 360 Digital time setting 1 min 24 hrs non stop increment 1 min Maximum continuous operation time 8 hours Overall dimensions 325x190x155 mm WxDxH Weight 1 4 kg Recomended load 75 of the rated volume Input current power 12V 110 mA 1 3 W consumption External power Input AC 100 240 V 50 60 Hz supply Output DC 12V ORDERING INFORMATION Cat number Bio RS 24 BS 010133 AAG with standard platform PRS 22 Optional platforms PRS 22 BS 010117 FK PRS 4 12 BS 010117 AK PRSC 18 BS 010117 EK Tube Volume Tube Diameter 1 5 15 ml 10 16 mm PRS series platforms are equipped with universal rubber clamps for different size tube fixation PRSC series platforms have metal clamps able to hold heavier solutions e g soil sand prs 22 prs 4 12 PRSC 18 1 14253 P 5 1 21412345 P DESCRIPTION SPECIFICATIONS Devices Rockers Shakers Rotators Vortexes Multi Bio RS 24 Multi Rotator Programmable Rotator Multi Bio RS 24 performs several motion types in one module Microprocessor control allows performing not only Vertical rotation of the platform but also Reciprocal rotation as well as 8 Vibration These three motion types can be performed separately pairwise and in cycles periodically repeati
145. was directed to the jar and the side without marked to the vacuum source eo um Product video is available on the website 2L Optional 8 channel adapter manifold MA 8 Exchange The filter is necessary replaced as it is glazed with dirtiness or by liquid sucked Maximum using time is 30 days After ending of suction of infection patient we recommended immediately exchanged the filter It prevents spread of infection Vacuum 500 mbar Trap flask s volume 1 litre Dimensions 160x210x340 mm WxDxH with trap flask Weight with trap flask 1 7 kg Input current power consumption 12V 300 mA 3 6 W External power Input AC 100 240 V 50 60 Hz supply Output DC 12V ORDERING INFORMATION Cat number FTA 1 with 11 trap flask BS 040108 AAG Optional accessories MA 8 BS 040108 BK Replacement parts Suction microbiologic hydrophobic filter BS 040108 S25 Laboratory Furniture 105 Laboratory Furniture Table T 4 LF 1 laboratory chest of drawers Table T 4L P Table T 4L with PCR cabinet UVT S AR 2 chests of drawers LF 1 and equipment for nucleic acid extraction T 4 Weight 23 kg Table with shelves T AL P chests of drawers LF 1 and equipment for nucleic acid extraction Overall dimensions W x D x H 800x600x745 mm T AL ed Overall dimensions W x D x H 1290x600x770 mm T 4L P Weight 54 kg Overall dimensions W x D x H 1290 x 600 x 16
146. x 380 mm OPTIONS AND ACCESSORIES Labwise PC software optional Allows two way communication for status display programming and data capture see p 16 1 for more information External probes optional TXPEP flexible plastic probe 3 m cable TXSEP stainless steel probe 3 m cable Remote switching device optional For switching appliances on and off up to max 8 Amps 1 2 Vertical turbine pumps optional Low noise compact design Supplied with pipe connections and special lid for fitting to tank pipe bore 12 7 mm VTP 1 max pressure 1 000 mbar aF max flow 9 L min VTP 2 max pressure 1 650 mbar max flow 12 L min Required only where application demands a higher pressure than that delivered by the internal pump to maintain flow ORDERING INFORMATION catalogue number matches the name of the product 8 Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heating cooling Circulators Optima Series Water Bath Accessories stainless steel flat stainless steel ES ACCESSORIES Lids Polypropylene Racksystems Raised Accessory cooling systems to help reduce spheres no of packs tO optimise use shelves to allow systems to operate at or below room evaporation heat loss required of available temperature by means of a cooling coil dipped M bath capacity to allow shallow temp COl App and
147. x W mm E ORDERING INFORMATION General purpose Digital T100 TC120 0 05 0 05 0 1 3 0 1 0 1 0 1 4 digit LED 1 to 6000 mins 3 3 fixed high without relay 1 3 1 3 1 4 50 60 Hz 1 4 50 Hz 200 200 135 135 210 16 m 6 11 TC120EURO T100 EURO Digital TX150 0 01 0 05 200 High Performance TXF200 0 01 0 05 0 1 0 01 with Labwise full colour QVGA TFT 1 min to 99 hrs 59 mins 3 USB amp RS232 remote via PC 1 program 30 segments 1 3 USB amp RS232 direct via user interface or remote via PC laptop 10 programs 100 segments 1 adjustable cut out high and low 1 9 2 0 50 Hz 200 310 18 6 11 315 145x115 TX150 EURO high and low 1 9 2 0 50 60 Hz 200 135 530 23 adjusted flow rate 6 11 TXF200 EURO Thermostatic equipment Water Baths Orbital linear shaking Baths Unstirred Water Baths Heatina cooling Circulators Optima Series Water Bath Combinations and Accessories TC120 Temperature setting range TX150 Temperature setting range TXF200 Temperature setting range Capacity L Outer tank dimensions Working area Lx w T100 e Min max liquid depths Temperature e Inner tank dimensions Lx Ww x setting range e Overall dimensions incl controller Lx W x H ST5 5L 150 150 mm stainless steel 3 kg 85 140 m
148. xing and Orbit temperature control of samples in microtest tubes or PCR plate A PT This model of Thermo Shaker differs from TS 100 with a possi bility of cooling samples down 4 Features of TS 100C meet e the highest expectations of users according to many parameters A 1 Fast reaching of specified mixing speed and maintenance of J equal amplitude of rotation throughout the Thermo Shaker block 1 2 Stability of maintaining the preset temperature in a wide range throughout the Thermo Shaker s block surface Z 3 LCD display indicates preset and current values of tempera ture speed and time of operation 4 Quiet motor operation compact size prolonged service life 23 Functions of heating and mixing can be performed both simulta Product video is available on the website neously and independently There are five heating and cooling blocks available including GM Mixing Efficiency Video is available on the website block with a plastic lid for the PCR plates All blocks are A mutually interchangeable and can be easily installed on Thermo Shaker Pictures and description of the blocks are provided on the page 32 HEAT UP AND COOL DOWN TIMES FOR TS 100C E The instrument is applicable in from 25 C Genetic analysis in extraction of DNA RNA and further sample preparation from 100 C to 25 C e Biochemical study of enzymatic reactions and processes e Extraction of metabolites from cellular ma

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