Measuring the Dissolved Oxygen of Wine in the
Contents
1. to the following as needed e Export Data On e Data Log On e Date Time Set current date amp time Sensor Performance Checks A properly calibrated RDO sensor should read between 98 and 102 saturation in the calibration sleeve If not recalibrate the sensor The RDO sensor should stabilize during calibration within 2 minutes when working properly Make sure to thoroughly rinse and blot dry the DO sensor after measuring samples and before placing into the calibration sleeve see Comments section Refer to the RDO sensor user manual if the sensor does not pass the performance checks Sensor Rinsing Soaking and Storage After each sample measurement rinse the RDO sensor thoroughly with deionized water and blot the sensor dry with a lint free cloth For short term storage overnight or between measurements keep the RDO sensor in the calibration sleeve or a biochemical oxygen demand BOD bottle with water saturated air For long term storage keep the RDO sensor in the calibration sleeve Sample Preparation and Preservation No sample preparation required Dissolved oxygen can be measured directly in the wine bottle Calibration If not already done prepare the RDO according to the RDO Sensor Setup procedure Perform a water saturated air Air calibration with the RDO probe in the prepared calibration sleeve A stable reading of 100 0 saturation should be displayed within about two minutes Analysis Slide a ring of2. Key Words Wine quality dissolved oxygen RDO sensor portable meter beverage testing Goal Measuring the Dissolved Oxygen of Wine in the Bottle Water Analysis Instruments Thermo Fisher Scientific The following application note describes how to reliably measure the oxygen content of wine directly in the bottle using a Thermo Scientific Orion Rugged Dissolved Oxygen RDO Sensor with automatic temperature compensation and a Thermo Scientific Orion Star A223 RDO DO portable meter Introduction A bottle of wine s oxygen content has a great effect on its quality stability and longevity This is why monitoring and controlling the oxygen incorporation at different stages of the wine making and bottling process is becoming a growing concern for wineries Although oxygen is a part of the wine s natural aging process adverse levels can cause discoloration to white wines and flavor degradation to both white and red varietals To ensure the consumer is getting the highest quality product measuring the concentration of molecular oxygen of wine after bottling is very important By using a Thermo Scientific Orion RDO optical dissolved oxygen sensor and a Thermo Scientific Orion Star A223 RDO DO portable meter reliable oxygen measurements can be made directly in the bottles of wine STO a 0N uoesnddy Equipment e Orion Star A223 RDO DO Portable Meter Kit includes RDO optical DO sensor portable meter arm
3. d for RDO Sensors 087002 RS232 Computer Cable 1010053 thermoscientific com water 2015 Thermo Fisher Scientific Inc All rights reserved Microsoft and Excel are trademarks of Microsoft Corporation All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Water Analysis Instruments North America Netherlands India Japan Te Eime See esea Thermo Vee erent China Singapore Australia SCIENTIFIC Gel ess ae ae A al awe ae leat 1300 735 295 A Thermo Fisher Scientific Brand InfoWaterAU thermofisher com AN DOWINEBOT E 0215 RevA
4. e RDO sensors do not require stirring or a sample stream for accurate measurements The speed accuracy and precision of the RDO sensor are equivalent or superior to the traditional polarographic sensor measurement If readings are slow or inconsistent ensure the temperature sensor is completely submerged in the sample If the temperature sensor is not in the sample the DO readings will be incorrect The RDO cap must be replaced every 365 days The remaining RDO cap life can be viewed in the RDO DO channel setup menu The meter will display an error message when the RDO cap needs to be replaced Oxygen mg L a ee a a Sample 1 0 35 0 17 0 80 0 64 Sample 2 0 46 0 32 1 61 2 01 Sample 3 0 34 0 26 1 39 1 04 Temperature C 21 5 21 21 3 2i 2 5 E Polarographic 20 E 200 5 1 5 S 1 0 0 5 Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 White Wine Red Wine Summary Using an Orion Star A223 RDO DO portable meter with an RDO optical dissolved oxygen sensor enables wineries to continually produce high quality wines Because the RDO sensor allows the wine to be measured directly in the bottle dissolved oxygen measurements can be made with speed and accuracy The speed accuracy and precision of the RDO sensor is equivalent or superior to current DO measurement techniques Visit www thermoscientific com water for additional information on Thermo Scientif
5. ic Orion products including laboratory and field meters sensors and solutions for pH ion concentration ISE conductivity and dissolved oxygen analysis plus spectrophotometry colorimetry and turbidity products gt v v A w ot zZ o m VI To purchase an Orion Star A223 RDO DO portable meter Orion RDO sensor and other related products please contact your local equipment distributor and reference the part numbers listed below Product Description Part Number Thermo Scientific Orion Star A223 RDO Dissolved Oxygen Portable Meter STARA2230 Thermo Scientific Orion Star A223 RDO Dissolved Oxygen Portable Meter Kit with STARA2235 RDO Optical DO Sensor Portable Meter Armor Field Case and USB Computer Cable Thermo Scientific Orion Star A326 pH RDO DO Portable Meter Kit with ROSS Ultra Portable Meters Low Maintenance Gel pH ATC Electrode RDO Optical DO Sensor Portable Meter STARA3265 Armor Field Case Calibration Solutions and USB Computer Cable Thermo Scientific Orion Star A329 pH ISE Conductivity RDO DO Portable Meter Kit with ROSS Ultra Low Maintenance Gel pH ATC Electrode Conductivity Sensor RDO STARA3295 Optical DO Sensor Portable Meter Armor Field Case Calibration Solutions and USB Computer Cable RDO Sensors Thermo Scientific Orion RDO Sensor with 3 Meter Cable 087010MD Calibration Sleeve for RDO Sensors 087003 Accessories Stainless Steel Protective Sensor Guar
6. or field case and USB computer cable Cat No STARA2235 or e Orion Star A223 RDO DO Portable Meter Cat No STARA2230 or equivalent Orion portable RDO meter e RDO Optical DO Sensor includes calibration sleeve and stainless steel sensor guard Cat No 087010MD e Silicone tubing Solutions e Deionized water DI Luminescence Based Dissolved Oxygen Method The oxygen content of wine must be monitored throughout the wine making process Using the RDO sensor with built in automatic temperature compensation and a portable meter reliable measurements can be directly in the wine bottle RDO Sensor Setup Refer to the RDO Optical Dissolved Oxygen Sensor User Guide for detailed assembly and preparation instructions for the RDO sensor Place RDO sensor into the calibration sleeve Remove the sponge from the bottom of the calibration sleeve Moisten it with DI water squeeze out excess water and replace the sponge Connect the RDO sensor to the 9 pin MiniDIN input on the meter Once assembled the RDO sensor can be used immediately Meter Setup Turn the meter on The meter should automatically detect the type of DO sensor and update the measure type to RDO Access the setup menu and update the RDO DO channel settings to the following as needed e Measure Mode Auto e Measure Unit mg L e Resolution 0 01 e Read Type Auto Read e Baro Pressure Auto e Salinity Correct Manual 0 0 Update the instrument settings
7. rmoscientific com OrionMeters Use the Orion Star Com software to export data to a Microsoft Excel spreadsheet or as a comma separated value file csv or print data to a network or local printer Quality Control QC Recommended QC procedures may include calibration check of the thermistor temperature sensor response against a calibrated NIST traceable thermometer and measurement of a zero DO solution such as 5 sodium sulfite Notes Silicone tubing is necessary to make a seal so the bottle can be laid on its side immersing the probe and temperature sensor in the sample while keeping all of the wine in and ambient oxygen out of the bottle The RDO sensor and temperature sensor are not immersed in the sample if the bottle is sitting upright Use a piece of soft silicone tubing with an inner diameter of 1 2 to 5 8 inch and outer diameter of 5 8 to 3 4 inch with a wall thickness of 1 8 inch Cut a ring of tubing that is 1 4 to 1 2 inch wide Before measuring the wine sample in the bottle slide the ring onto the probe and push it up to just below the threads Keeping the calibration sleeve clean and free from water or sample droplets is essential to getting good calibration and read back values in water saturated air Rinse the RDO sensor thoroughly with deionized water and wipe excess water with a lint free cloth prior to putting the sensor in the calibration sleeve Results Dissolved Oxygen Readings in Wine Bottles
8. silicon tubing over the RDO sensor sliding it up the probe to just below the threads For details see Notes below Rinse the RDO sensor with deionized water and blot excess rinse water off with a lint free cloth Place the RDO sensor in the bottle The silicone ring should make a seal with the bottle Place the bottle on its side so the neck becomes flooded with wine covering both the dissolved oxygen and temperature sensors on the RDO probe Initiate a reading using the Auto Read measurement mode by pressing the measure key on the meter keypad For best results take a second reading to ensure the dissolved oxygen measurement is fully stabilized as it may take the RDO sensor one to two minutes to fully stabilize in the wine sample Use the second stable value for the oxygen content of the wine Both readings will be saved in the meter data log Comments It is important to thoroughly clean the RDO sensor after sample measurement Rinse with deionized water and thoroughly blot all excess water with a lint free cloth several times before putting the sensor in the calibration sleeve Rinsing following the completion of all sample measurements should take 5 to 10 minutes The Orion meter data log collects up to 1000 measurement sets with time and date stamp and the non volatile meter memory preserves data even with loss of power Download Orion Star Com software to facilitate the transfer of the data log from the meter to a computer at www the
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