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1. 59 128 no ee E i 2 PE JAE Haha Display Saturation A saturated image is inappropriate for image quantification with image analysis software The saturation option allows you to visualize in red pixels that have the maximum grey level 4 095 in order to avoid to flatten the peaks To proceed select the saturation option The saturated pixels are displayed in red Note If an image is being acquired and the Saturation option is checked the modification is applied to the current acquired image Note A saturated image creates quantification error when studied by an image analysis software Gel doc systems have to indicate to the user if the image is saturated and if it is then necessary to modify the integration time Display Minimum Maximum Default The Default display windows allows to gt monitor the image dynamic modify the greyscale selection to enhance the image display on AutoScale Minimum Maximum em Gamma Ci Saturation S 1 00 DISPLAY The acquired images are for instance 12 bit ones ranging from 0 up to 4 095 grey levels Windows can only display 8 bit images 256 grey levels Due to this limitation for each acquired image the UVIsave 1D software handles 60 128 Bl Pee 2 a P pnp D a2 via erat two images gt a memory image corresponding to the image acquired 4 096 grey levels gt a display image corresponding to the image display
2. TIF 1M00000311 i imo00055555 1M0000 12222222 i ircsSmin 30sec irsc10min 2 Cancel 4 Enter the desired file name select a file extension and validate Q 2 pea i m00000111 i im000124 i im000135 i IM00000311 Save in Molecular weight IM000013 1M000020 i im000033 im000043 igducchini Igrs 30min Igrs 30min 8bit ig wonkygel 1M000004 i imoo0006 IMO00011 1M000012 i imo00053 i imooo0s9 i im000073 i imoo0092 i imoo0055555 i IM000012222222 ircsSmin 30sec i irsc10min Note the software proposes a default file name imO0000x If the default file name is selected it will be incremented by one each time an image is saved 117 128 PT PRS Pee JAE hahaa 1 Click on the Print icon A pop up window displays the following menu EPSON Stylus C70 Series Print range Al Choose a printer Click on Properties to modify the default setting of the printer if necessary gt Select the number of copies Click on OK to validate your options Note You can also access the Print menu from the Menu bar File Print 2 The Print preview displays a preview of the image as it will be printed To proceed select File Print Preview from the Menu bar Closa Save Print Setup R 1 1M000012 2C WLIMAGESM000001
3. 110 128 d i pPE JEE D 3t2t trata Parameters ars Image display parameters Min number of pixels Default value Default gamma value 1 Optimum display Minimum number of pixels parameter The optimum display is controlled by a parameter Min number of pixels The minimum number of pixel is the minimum number of pixel of the image at a given grey level used to determine if a grey level is significant to be used as a reference Starting from grey level value 4 095 the software searches for the first significant grey level number of pixel upper than Minimum number of pixel variable If the number of pixel is higher or equal to the value defined as parameter the grey level is used as a reference for image display calculation If not the software analyses the lower grey level values until something significant is found Note You can always come back to the default values by clicking on the Default values button 2 Default gamma value You can define the default gamma value used to display the images Gamma adjustment corrects an image by creating a new version of the original To create the new image the Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the following graph 111 128 EPs TEE sat strata full scale gamma lt 1 Pixel Value New Image full scale o Pixel Value Original Image The above graph demonstrates the
4. amp L Cancel Select the drive by using the up and down keys KA EA Then select the Files option using the left and right keys Ea EA Select a specific file by using the up and down keys SHL OK P Validate using the OK key Note By pressing the Cancel key you could return to the image acquisition mode Note An image thumbnail is displayed when a file is selected Note Only the images of the default image format are displayed 42 128 Peo HREN a i i D saat utara Set date and time Set date and time The UVISAVE HD2 has a clock that records the date and time at which you take each picture The information is recorded in the image property of the Good Laboratory Practice GLP file To change the date hour and time zone you need to unlock the system Please refer to the Unlock chapter of this manual When unlock you can set the date and time from the Option folder of the Set up menu The following display will appear Current date Wednesday 8 July 2009 Time zone GMT 01 00 Brussels Copenhagen Madrid Paris Time o 2 24 a5 K DB E cmu Select the date the time zone and the time by using the left and right keys Ea EA Then select the appropriate option using the up and down keys K EA OK lt Validate using the OK key 43 128 i JF Aa utr wt Unlock i e Add a printer The UVISAVE HD2 sy
5. ntensity Min Max gE DISPLAY GETE C geass pre When you are merging monochrome images the program uses whatever pseudo colours that you have set for that image So if you defined a specific palette for an image that colour appears in the merged image If a palette has not been assigned to an image the program uses the greyscale palette as a default Palettes cannot be applied to monochrome images through the Merge images function They must be applied prior to merging the image For information on defining pseudo colours refer to the Pseudo colours option of this manual in the Image enhancement chapter 3 Click on merge images to obtain the target image 115 128 LPS Pee 32 vse TOOLBAR IN DETAILS Opening an image This function opens an image file of a specified format i e TIFF BMP GIF JPEG 1 Click on the Open icon A pop up window displays the following menu Look in 4 Molecular weight a Ee Ej lm IMoo0004 i imo00033 i 1M00000111 imooo006 i imoo0043 lm imoo0124 lim mooo011 im000135 moooo12 lw imooooss E IMO0000311 IM000013 imoo0073 imoo0055555 lm imooo092 IMo00012222 Px G My Documents A Mes images G Y My Computer A 3 Floppy 8 a Local Disk C ds CD Drive D Sa IOMEGAMINI E Ga Shared Documents la My Documents aj My Network Places C Unused Desktop Shortcuts Catalogue 3 Double click on the i
6. 2 F gt ie Live X _ Auto Next Note The image is printed according to the Display option If the date and time bar is displayed the image will be printed with the date and the time stamped on the print 40 128 eo Te a a es D JAEVHARTAN Open an image You can open an image from the internal memory or from your USB key drive for further printing To process press Open an image Open an image aie A pop up window displays the list of drive and the list of images for the selected drive Drive External drive 510 MegaByte free Files K OD DBD Gq cance Select the drive by using the up and down keys KA EA Then select the Files option using the left and right keys Ea EA Select a specific file by using the up and down keys KA EA lt OK Validate using the OK key Note By pressing the Cancel key you could return to the image acquisition mode Note An image thumbnail is displayed when a file is selected Note Only the images of the default image format are displayed 41 128 Delete an image E AERPEJFPASEN D saat vrata Delete an image You can delete an image from your USB D drive for further printing To process press Delete an image Plats cn Wase A pop up window displays the list of drive and the list of images for the selected drive UVITEC Drive External drive 510 MegaByte free OK gt
7. Software is in use on only one computer at any time The Software is in use on a computer when it is loaded into temporary memory RAM or installed into the permanent memory of a computer for example a hard disk CD ROM or other storage device lf the Software is permanently installed on the hard disk or other storage device of a desktop or portable computer other than a network server and one person uses that computer more than 80 of the time then that person may also use the Software on a 2nd computer for the purpose of viewing and post processing images Title We remain the owner of all right title and interest in the Software and Documentation You will agree to use your best efforts to protect the software from unauthorized use illegal reproduction or illicit distribution 10 128 D JAFHA A Copyrights and Trade Secrets UVItec owns all rights to the Software This agreement does not convey ownership of the software to you but only the right to use a copy of the software strictly in accordance with this agreement The unauthorized duplication or distribution of the software or the documentation is an infringement of UVItec rights to the software and may be subject to criminal and or civil penalties Archival or Backup Copies You may either e Make one copy of the Software solely for backup or archival purposes provided that the original copyright notice and or other legend is reproduced on each copy or e Transf
8. This license agreement is governed by the British laws Copyright 2006 2010 UVitec Ltd All rights reserved Windows Vista Windows XP Windows Excel Windows Office are trademark of Microsoft Inc All other product names mentioned in this document are the property of their respective trademark holders 11 128 fab a Ee E T D tat strata UVISAVE HD2 INTRODUCTION Easy and fun UVIsave HD2 is a stand alone imaging system dedicated to basic gel documentation Thanks to its built in USB media drive the images can be saved on a USB stick or printed on a thermal printer for instant hard copy images UVIsave HD2 combines simplicity and economy for laboratories with budget in mind Key features No computer required for image acquisition or printing Publication and quantification of high quality images What you see is what you get 12 bit imaging Highly sensitive CCD camera USB key saving media Pixel saturation warning All the necessary information to ensure the optimum quality of your image Compact and robust darkroom TIFF image format Good laboratory practice file GLP Complimentary UVlsave 1D software 12 128 ee ee oe ee D a2 strantan UVISAVE HD2 HARDWARE INSTALLATION Unpacking the UVISAVE HD2 system Do not handle the system by the camera or the camera support Please open the UVISAVE HD2 box carefully and verify the contents UVISAVE HD2 Darkroom Camera and camera support Power c
9. basic principles of gamma adjustment Black pixel value 0 remains black at all gamma values White pixel value full scale remains white at all gamma values Gamma values greater than one lift the darker areas of the original image into the brighter areas of the new image A gamma curve is smooth there are no unexpected jumps or cut offs This means that when viewing a gamma adjusted image you will be able to see the details intensity differences in both the black and white areas of the image When the bright areas of these types of images are correctly exposed the darker areas can be so dark that they are in effect invisible Gamma Adjust can remedy this problem The gamma adjustment results in a better display of detail by lightening the darker areas without burning out bright areas or lightening black areas 112 128 JSePetPegee a2 strsetAnt Good laboratory practice GLP 1 The Good Laboratory Practice GLP file is made to track all the image treatments performed with the software Click on the GLP icon A pop up window displays the following menu Good Laboratory Practice GLP Experiment tite Comments Glp contents Image manipulation Gray level inversion No Contrast adjustment from previous image Brightness adjustment from previous image Image movements o Cancel Printout Enter the experiment title and comments Enter zoom parameters aperture zoom
10. detection range The new detection parameters are displayed and a preview of the detection is displayed on the image Note If Colony count count button has been done on the image the modification of the area will cancel it It will be necessary to press once again the count button to access the results 4 Click on the Count button Count 87 128 i DPE SP eee D JAER Once the preview of detection is satisfactory click on the Count button The colonies are counted and their parameters are displayed in the bottom part of the screen b owes ee rf A Cova on Csere totems s Pa 4 i sioner s oe ory Wma of s steers yasar 168 Oy leol satona ner Tetesi eavtece 18 Orire c 175 oo nee we yr ws we Mw ee p p p jg eS A D SESFESEEELES A w Pi rir ee Sw KATIE Shooudises p ARSE SETE nE TE i n SEIE SE ne i E nE nE a a a E e E e ee o e o e o oil e aah er l a a i i ete ee ee es ee FEFESEEETELE gt men Note For each colony the parameters are gt Number number of order of the colony gt Gravity co ordinates of the centre of gravity of the detected colony gt Volume sum of the grey level of the pixels constituting the colony gt Area number of pixel constituting the colony gt Perimeter Circumference of the colony gt Compacity Compacity coefficient C 4nS area 0 lt c lt 1 p2 perimeter gt Eccentricity S
11. from the Menu bar 80 128 i DPS Pee JAE Haha Cti 0 Citi 5 Ctrl P Preview h Print Setup 1 M0000012 2 CWUMAGESNMMOO0ON Exit A pop up window displays the print preview Selection of window Frofile or marker view J oau gt Select the window to be previewed Note please refer to the Printing the results chapter for an explanation of Windows 1 2 and 3 A pop up window displays the print preview gt Click on Print to print as previewed gt Click on Close to close the Print preview and to go back to the main menu 3 The Print Setup allows you to choose a printer and to configure the printing To proceed select File Print Setup from the Menu bar 81 128 12D PE SP ee ee D JEWISH File Open Cti o Close Save Cirl 5 Print Ctr P Print Preview Print Setup N 1 M0000012 2CVLIMAGESIMO00001 A pop up window displays the print setup menu Print Setup Printer Name SSeS een snes v Status Ready Type EPSON Stylus C70 Series Where USB001 Comment Paper Orientation Sie A4 210x 297 mm J Portrait Source Bac feuille 4 feuille v Landscape gt Select a printer gt Click on Properties to modify the default setting of the printer if necessary gt Select the paper size and source select the orientation Click on OK to validate your options gt Note After you have installed and setup your printer
12. reboot automatically This procedure last few minutes Please do not try to access the system during the updating time USB device precaution DO NOT PLUG OR UNPLUG A USB KEY DRIVE WHILE THE CAMERA IS ON USING THE LIVE MODE OR THE EXPOSURE MODE 50 128 PES Pe 2 eo i f Jat strat UVISAVE HD2 eu ikea F N Taking your first picture gt Switch on the UVISAVE Click on to position your sample and to adjust the zoom the aperture and the focus gt Switch off the white light and switch on the UV light Click on keys to adjust the exposure time Freeze your image with the Switch off the UV light Save your image with the Print your image with the 51 128 AREA A UVISAVE HD2 USING THE UVISAVE 1D SOFTWARE UVIisave 1D operating environment The UVISAVE HD2 is supported by the UVisave 1D software Power on the orig After the computer has booted up completely double click on the UVISAVE 1D icon The UVIsave 1D software opens to the following image window EXPAND YOUR TERRITORIES UVISAVE UVILES 2 Sy UG Bercsa Open an image and a folder menu appears on the left hand side of the display 52 128 i PES Pee ee IAEA Open an image and a folder menu appears on the left hand side of the display Ke UVIDoc bichat KER Gr Edi Imacesnsusre Testienhencemert Corvols amp peraneters Window Help ax s Acquisition Open e Save Prnt A Crop Gj Clipboard an U
13. the largest possible within the gray scale of the system gt 4096 gray levels for 12 bit imaging The gray scale is the total number of levels between white and black The image dynamic range within this scale Adjustments are necessary to obtain an image with the broadest dynamic With most of the gels a real time image has a limited dynamic In this case the exposure time enhances the image dynamic In order to obtain an optimum image you can control the image dynamic by adjusting the exposure time The image dynamic is displayed in the Amplitude bar graph as shown in the illustration below AMPLITUDE EXPOSURE 08 08s 2222 gt 2 gt 2 gt 2 gt 2 2222 2222 22227 Live r O Freeze Auto Illustration of the use of the Dynamic bar graph 28 128 poe w aa j HPE AaE uaa AMPLITUDE EXPOSURE HHH E 2222 2222 2222 2222 2222 Live Wrreeze _ Auto AMPLITUDE EXPOSURE gt 2 gt 22 gt 2 gt 222 gt 2222 2222 2222 A Live a O Freez Auto Increasing the exposure time enhances the image dynamic 29 128 L PPT Pee se WAREAH The freeze key stops the exposure process and captures the last image for further saving or printing To proceed click on the Freeze key EZ to stop the image acquisition The monitor displays the last full exposure Note By pressing the Live key or the exposure time keys the image is released from the freeze status The following scre
14. the procedure for setting up and configuring a printer is the same as in other Windows program 82 128 DIFESE Jere D JAEVWARIAH Autofit Click on the Autofit to resize the image to fit the size of the monitor The full resolution of the acquired may be larger than the screen resolution The navigation requires the Windows scroll bar The Autofit allows you to view the whole image regardless of the window size Typically reducing the size of a window also cuts off part of the image The Fit to Window option solves this problem by resizing the image so that it is always the same size as the window The Autofit feature proportions the display of the image to the screen resolution Autofit no scroll bar No Autofit scroll bar Default display The Default display windows allows to ic monitor the image dynamic modify the greyscale selection to enhance the image display DISPLAY Minimum Maximum 14337 F Inverse Gamma Ci Saturation 100 The acquired images are for instance 12 bit ones ranging from 0 up to 4 095 grey levels Windows can only display 8 bit images 256 grey levels Due to this limitation for each acquired image the UVIsave 1D software handles two images gt a memory image corresponding to the image acquired 4 096 grey levels gt a display image corresponding to the image displayed on the screen 256 grey levels The easiest way to calculate t
15. 020 i Igrs 30min abit i im000033 ig wonkygel i imo00043 i im000058 im000059 i im000073 i imoo0092 i 1M00000111 im000124 im000135 1M00000311 i im000055555 IM0000 12222222 ircsSmin 30sec irsc10min lt Il File name IM000002 N Save as type Tagged Image File Format TIF J TIEF pamar Zev compressed TIF Bitmap Files BMP BMP compressed BMP Targa Files Tga PCX Files Pcx Mac PICT Files Raster Pic Joint Photographic Experts Group Files JPG Graphic Interchange Format Files GIF WordPrefect Graphic Files Wpa Saving the user settings 68 profile in You can save and load your personal software configuration The acquisition parameters as well as the software configuration can be saved for each user To proceed click on the Profile in icon A pop up window displays the following menu Save As Save in Ci VLCONF Save as type Config File CNF 2 Enter the desired file name and validate Note the user setting extension file is CNF 121 128 Jeni THID D a2 WARTAN Loading the user settings Click on the Profile out icon A pop up window displays the following menu Files of type Config File CNF 2 Browse to specify the file name and validate Click on the Contextual Help icon The standard mouse cursor is changed to the following cursor Wi
16. 2 128 tesa RA Display of the profile from the minimum value to the maximum value of the lane 103 128 noe TE E rr Jere D sat strawtan Printing the results 1 Click on the Print icon A pop up window displays the following menu Selection of window Profile or marker view Array of result view gt Select the window to be printed 2 Click on OK to validate your choice A pop up window displays the following menu ES eo v Ready Type EPSON Stylus C70 Series USB001 Comment Print range All gt Select a printer Click on Properties to modify the default setting of the printer if necessary gt Select the number of copies Click on OK to validate your options Note You can also access the Print menu from the Menu bar File Print 3 The Print preview displays a preview of the image as it will be printed To proceed select File Print Preview from the Menu bar 104 128 LP creer Era at 1 IMODO012 2C WLIMAGESUM000001 Eat Selection of window Array of result view oc cancel O gt Select the window to be previewed A pop up window displays the print preview Click on Print to print as previewed Click on Close to close the Print preview and to go back to the main menu 4 The Print Setup allows you to choose a printer and to configure the printing To proceed select File Print Setup from the Men
17. 5A 1 FS TC20 CM 20M o del e UVISAVE HD2 NIS 515A FS TC26 CNX GENERAL ADVICE e To clean the surface of the filter use a mild solvent or warm water Dry with a soft cloth e The filter is porous so try to keep it dray TYPE OF FUSE All our units are fitted with one or two safety fuses They are Type FST found in the plug at the rear of the unit Time lag T O5 x 20 125 128 i e L T ZEE D JIE WAREAH UVISAVE HD2 WARRANTY Our products except Compact Flash light tubes and filters are warranted against faulty construction or defective material for a period of TWO YEARS from the date of supply Our products are not warranted for damage due to carelessness incorrect use or bad maintenance The following defects are also specifically excluded Defects caused by improper operation Repair or modification done by anyone other than UVITEC or an authorized agent Corrosion caused by improper solvents or samples Use of spare parts supplied by anyone other than UVITEC Damage caused by accident or misuse Damage caused by disaster This instrument should not be modified or altered in any way Modification or alteration of this instrument will 1 Void the manufacturer s warranty 2 Void the conformity certifications 3 Create a potential safety hazard The Compact Flash the tubes and the filters are not cover by our warranty The use of consumable products or non original spare parts not recommen
18. AGE ANALYSIS MOLECULAR WEIGHT Accessing the quick image analysis menu Select the Image analysis folder From the Image analysis folder select the Molecular Weight option OD _ Image analysis Distance calculation RF E Colony counting A new icon bar appears n ms m Lane Band Ly Warker a Ge cenar voll hea a Print E definition Mls detection MPS values fare Results hn Volume Autofit 2 Help display analysis 69 128 Jerez Paegee D a2 strata Step 1 Define the number of lanes Lane 4 definition Click on the Lane definition s Lane Band Marker Default Exit Print 4 definition t2 detection 3 values als Results he Volume display N Autofit Z Help analysis A pop up window displays the following menu emana r Lo aa Lane direction Vertical lanes 9 Horizontal lanes Total lanes Detect the bands Cancel ap i al Choose the direction of the lanes from gt horizontal gt or vertical Lane direction Yertical lanes Horizontal lanes Select the number of lanes Total lanes Kamm gt gt On the image click and drag to define the analysis area and to overlap the lanes gt You can adjust the size of the area by clicking on the tags surrounding the area and drag the selected border to the requested size Once the lanes are properly defined click on Detect to trigger the detection Detect th
19. E 39 A eE A E A E E E E E E E E E 40 f RPE ZEE D Aae Trae S181 212 1 THINS E E decorate A T E EE E E A EE 40 Do IEE ni Se PEES spear vnc E E A AE A E A E E E E E A 41 D Sae E1 2 E E A N E E E A E E E scons E N E T E A 42 AT a E AE A EEE EEO EEEN 43 JOCK LEAG ION O eesi EA TE AE EEA EE icine 44 PII EPE EE EAEE E AEE E E AE E E EE A EN 49 POWT EUO cra EEEE I EIEN OE A EET OA A NOA A O OE OAE 50 CLS VC COI eaaa AE AAE O AE EA ENEA 50 OOIE START aie E E E A E oust eenmnarit 51 TSAO TOU TST TOC I EE T E AT A TA EA A 51 USING THE UVISAVE TD SOFTWARE siiven iin n E E 52 VIS OVE 1D operating eNnViONMEnNt aeescicaneceussescuasescscsenevenavenceotecenceenauessscncepueneranauceaseqesd ponauestcsndosrennacosccedtonse 52 PIV E eng Te S10 Is sacas tate actreag cannon PE EA TEE TE 54 IVI CTI TVS TT CTO TOdo rerna uence gateraeeiarectoaeaeateem ats eeniers 56 PIV OCT TS POON E A EAE A E EAE E N 57 ES a E E E E 57 TAEC TATUS BAR iron a N E E A E E E ae T E E E EE 58 AOO ea EE E E A 58 TIN CLO STING ToT eiaa ooa RE AAE EAEE E EENE ENOO AEE EEEE EE AEEA EENE 58 DT A OS A A A E EE E E E R 59 BE E TV E E N A E E O AT O A A E P a remtaneteswouiass 59 BES e da Slt e a A AE AAA A EA PE A E ET 60 Display Minimum Maximum 7 Defa sisirin one n aE E E AEEA 60 EDISON ON i E E E A E A A T A A A E avarentne 61 EO Ge a A E E EEE E NEA A N EE ANT 62 Enhance Bnignmness 7 CONTAS api ae cetncstsectecaincetoantenaacris lt oncueheownaneecadoraeteacteasctqueaeteanaectas
20. E E aaneasaeis 90 AO ri A E E E EE E AEEA EEE 92 Bie TESE EE EI A AI EA A EA PEE A E E E 93 Returning to the image MAIN MENU sesessssssesssesssresoressrreseressresseressresseressressrresoressreeseressressrressressereseressrresere 94 MACE ANA AE R ec sarees ea E E T E T AEE E E 95 Open an image and select the distance calculation RF MENU s ssessssssssenssesessresssressrrsseressressrressres 95 Se 1 LEE TMG UMD Sl OF IONS norrir eoni i rira EA AAE OEA AOAIE EOE 96 Sep 2 IST SF Ihe NGS aie siscinerso nen sciee fis tarcconice NiE E ATE EAEAN EA ATENE 97 TEPS ASON INE RF VOUS aeeie enaA EEEE ast EE AEE EAE OEA 99 F i mee Zz E JAFHA AH Os TOS eS enres EE N E e a a AE raei 100 Jep S GUAN TNE SS utii nn cho achanetuer acta Gazette A aa scented ost ies AE SEAE 101 PEG FV SSIS E E E E A EE E E E E EEE E E EE AE 104 AO A EA E E EE AA AA E E E 106 Die L EEES E A E E TAE E E E E 107 Returning to the image MAIN MENU ssssssesssssessresseressressrresoressrrssrressresseressressrreseressreeseressressrreseressrreseressne 108 IMAGE ANALYSIS UVISOFT SOFTWARE ssssssssessssessesresresrosesrreresrosrerrereseosesreeroseoseereeresrosrereereseosrereeresroseereereseoseereereseeseete 109 Sending the image to the UVIDANA analysis SOF WOTE cssssccssssccssssccsssccesssccessescsssescssescsssescseseeeseatsesees 109 CONTROLS NDIPARAIMIEUER 6x xcsisese sictanceesnensesteucnesaconerecncenacanesceeaessanensosteeansaecenpessnaccacesecsanneneaeeacessscma
21. FF After boot up the UVISAVE firmware will automatically start and the following screen will appear 2222 2222 22 gt Next 25 128 oe ae E i PES JAE HARD UVISAVE HD2 IMAGE CAPTURE Live preview 1 Live mode allows direct visualization of the image This mode enables you to adjust the zoom the focus and the aperture and to position your sample gt gt gt gt FD gt gt gt FD gt gt gt gt Live ee T Freeze Note A live image means the image displayed is refreshed every 1 30th of a second This short exposure time or frame is adequate for a variety of white light samples including protein gels and autoradiography However a live image is not sufficient for most samples which are visualized and photographed over a relatively dim UV light source A feature called exposure compensates the low light situation by allowing the CCD camera to obtain a timed exposure Note After 2 minutes the firmware will automatically stop the live preview Click on Live a second time or on Freeze to captures the last image 26 128 r LRP EIF ae D JAE WAREAH Exposure time The emission light signal coming from the gel could be relatively dim A feature called exposure time compensates this low light situation This allows the CCD camera to collect the incoming light over a greater period of time The exposure time is the acquisition of an image by summing the signal of the camer
22. ILIN 5343 Pu Based i 177834 2003 ime 160 00 Based 168633 1555 isnt Io Baad 103603 S69 460 Band 13632 4273 328 Band 19 2620 1433 Band 32206 tsa gt The volume is the sum of all the intensities included in the defined area window separation gt The height is the maximum intensity gt The area is defined for each peak by the width of the window and the separation lines 101 128 a L RFR Lee oe Se tae straw 3 You can define a quantification threshold To proceed gt Click on the Threshold button The threshold allows to distinguish the bands from smears on the lane gt Move upwards on the screen the horizontal line gt Click on the left button of the mouse to validate the values are directly displayed gt The defined threshold is automatically applied to all lanes The results are recalculated taking into account the threshold 2 ae g an le 14 16 Note You can select the scale of the lane s display Three scales are available Profile full values Profile 0 to maximum values Profile minimum to maximum To proceed select the scale from the Scale profile menu End of profile to display Profile minimum to masimum Profile 0 to masimum values Profile minimum to masimu 3 Display of the profile from 0 to the Display of the profile from 0 to the maximum dynamic i e 255 for a 8 bit maximum value of the lane image and 16 384 for a 14 bit image 10
23. JAE HAD tl UVITEC Cambridge UVISAVE USER amp SERVICE MANUAL 1 128 D 32 strata UVITEC Cam bridge Thank you Dear Customer On behalf of UVItec we would like to thank you for choosing the UVISAVE imaging systems In order to learn the capabilities of your UVISAVE system we kindly ask you to read this manual This manual details how to install and to operate the hardware and the software components UVItec is dedicated to your satisfaction and we will be pleased to answer any question you may have We are also very receptive to your suggestions Many of the new features and enhancements in this system are a direct result of conversations with our customers Please do not hesitate to contact us to let us know what you would like to see in the next version of this system You can contact us at the following addresses gt Email uvi uvitec co uk gt Contact address UVItec Limited Avebury House 36a Union Lane Cambridge CB4 1QB United Kingdom Tel 44 0 1223 568 060 Fax 44 0 1223 306 198 Do not hesitate to visit our website at www uvitec co uk 2 128 D JAFARA UVITEC Cam bridge Table of content WARNING AND SAFETY sinisnsesir r N eis winiusnrideneseesausesseeeneees 6 END USER LICENSE AGREEMENT sisiririnnicrrns pannen NEENA 10 INTRODUCTION irinen NNE crcnertuessmieetecgumnves dower 12 SS N eaten eas E a EE PE A E N TE AE PE E A geet 12 HARDWARE INSTALLA TION wisi siicsecsnewseccncanie
24. ONFORDID T corentena E A A EE eresueeeassuennens 128 5 128 i i Tp 70 32 utraerAt UVISAVE HD2 WARNING AND SAFETY Please read carefully these instructions before installing and operating the UVISAVE HD2 system Whenever you find this pictograph be sure to refer to this Manual It indicates an imminently hazardous situation which if not avoided could result in death or serious injury Whenever you find this pictograph be sure to refer to this Manual It indicates a UV radiation situation which if not avoided could result in serious injury WARNING The operator should wear appropriate safety glasses or a protective mask and gloves UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 6 128 32t uaa This product is safe to use when operated in accordance with the instruction manual This instrument should not be modified or altered in any way Modification or alteration of this instrument will void the manufacturer s warranty void the regulatory certifications and create a potential safety hazard The intended purpose of the UVISAVE HD2 is gel documentation The system is designed for research use in laboratory only UVITEC is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended or for any modifications of the inst
25. a during the time displayed under integration time button When the specified exposure time is reached the image is displayed on the video monitor The CCD continues to integrate the image and the displayed image is refreshed each time the set exposure time is reached You can modify the exposure time using the following keys to increase the exposure time to decrease the exposure time Note Exposure time is the integration of the image on the CCD sensor over a period of time The effect is similar to exposure time for a standard camera Note The maximum exposure time is 5 seconds Note The Live mode corresponds to an exposure time of 1 30 second Note The selected exposure time is displayed on the monitor while acquiring the image Note When the specified exposure time is reached the last captured image is displayed The camera continues to integrate the image on the CCD sensor updating the display whenever the specified exposure time is reached The Freeze button stops the exposure process The last full exposure is displayed Click on Freeze to stop the exposure time and to capture the last image The exposure time is displayed in the control bar of the monitor as shown in the illustration below 27 128 F Ln pE JEE D Aae treat AMPLITUDE cXPOSURE 2222 gt gt 222 2222 2222 22227 Live r O Freeze Auto Image dynamic The image dynamic is crucial for quantitative accuracy It should to be
26. a straight forward image a printed paper a business card Then focus till you obtain a very sharp image on the screen This procedure can be done with epi illumination light and a printed paper The focus point adjustment varies with the focal distance You may need to adjust the 3 settings of the zoom lens Aperture Aperture allows the CCD sensor to receive more or less light Closing the aperture decreases the amount of light coming to the sensor thereby making the image darker Zoom Zoom allows you to change the size of your sample on the image To proceed click on the In or Out The zoom automatically moves to the new value The zoom control will not affect the focus Focus Focus is needed to adjust the sharpness of the image Turning the ring clockwise or counter clockwise changes the focal point of the lens Diaphragm ring Zoom ring Focus ring In order to properly adjust the focus please proceed as follows 1 Adjust the diaphragm ring on aperture 4 2 Adjust the zoom ring on position 69 3 Put a sharp written paper on the transilluminator transilluminator has to be off 4 Open slightly the darkroom door to obtain a bright image on the display 5 Adjust the focus ring in order to obtain a sharp image At this stage the image must be sharp whatever the zoom ring position is Do not change the focus ring position anymore as far as you do not modify the focal distance The lower the f st
27. able Instruction manual UVIsave 1D software CD Rom inside the instruction manual gt Remove carefully each component from the box gt To remove the UVISAVE HD2 from the box please use the belts which surround the system and designed for this purpose gt Do not handle the system by the camera or the camera support Remove their protective plastic cover Do not connect the power cable to a power source until all connections are made The power source has to be grounded and protected by a circuit breaker The UVISAVE HD2 system is designed to fit a specific voltage Please check the voltage to ensure it corresponds to the UVISAVE HD2 specifications 13 128 tat straw Please keep an open area of at least 20 cm around of the UVisave HD2 to ensure a proper air circulation for the system The system should be located in an area free of excessive dust or moisture strong magnetic fields or ionising radiation It is also recommended that the ambient temperature be stable and within the range of 15 C to 25 C 20 C recommended and that the relative humidity not exceed 70 non condensing Ensure that all of the systems ventilation openings are free of interference Excessive heat buildup in the instrument may affect performance or cause operational failure Do not defeat any instrument interlocks they are designed to prevent user injury lt is compulsory to power down the system and disconnect the AC mains fro
28. area is immediately displayed on the image Note You can adjust the size and the position by moving the mouse cursor on the defined area to position the area click on the area and drag it to its new position gt to resize the area move the mouse cursor on one of the little square surrounding the area The mouse cursor is modified click and drag the edge of the area to its new size Any area Once this option is selected the previous area is deleted Move the mouse cursor on the image and click on the image to define the first point of the area Move the mouse to another place a line is drawn defining one edge of the area Click when the edge is satisfactory Repeat those steps until the area is defined Then click on right mouse button to finish the definition of the area Note It is not possible to move such an area Note Click on the SQUARE check box to obtain a circle instead of an ellipse or a square instead of a rectangle Note If Colony count count button has been done on the image the modification of the area will cancel it It will be necessary to press once again the count button to access the results 3 Define the parameters for the colony detection Colony detection parameters 33 lt Green lt 105 150 lt Red lt The green and red areas represent the grey level range used to determine both kinds of colonies gt Click on the coloured square and drag them to a new place to modify the
29. ation 18 128 DIFESE E R ae D JAEJHWARIAH Minimum computer requirements for the UVIsave 1D software Minimum requirement Bus PCI bus Intel chipset supporting bus mastering mode Processor Intel Core 2 Duo and upwards Ram 1 Gb and upwards Hard disk 10 Gb and upwards At least 1Gb free disk space least in order to allow software installation and image storage Monitor AGP card 1280 x 1024 in 16 millions colour mode 24 bit Upper Video card resolutions supported Video card with a refresh rate above 70 Hz Operating Microsoft Windows XP SP2 and upper 32 bit only system Microsoft Windows Vista SP1 and upper 32 bit only USB Port At least two USB port available UVITEC cannot guaranty the correct working of the computer and the software when the BIOS energy saving options are active Windows is a registered trademark of Microsoft and must be installed before installing the acquisition board and the UVisave 1D software Do not plug or unplug a USB key drive while the camera is on using the live mode or the exposure mode 19 128 i DPR EP eee JAE VPA Software installation Step 1 gt Ensure all application programs are closed Windows XP and Windows Vista users should also ensure that they are logged on with administrator privileges gt Insert the CD ROM in the CD ROM drive gt If the Autorun option is set on your computer the UVIsave 1D installation will start automatically If not double clic
30. commercial court of Cambridge United Kingdom shall be competent even in third party claims proceedings or when there are several co defendants NOTE UVITEC is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by UVITEC Decontamination collection and elimination of waste The buyer ensures and finances the decontamination the collection and the disposal of waste electrical and electronic equipment WEEE under the conditions provided in the Articles 21 and 22 of the Decree No 2005 829 dated of 20 July 2005 Improper disposal may be harmful to the environment and human health 127 128 i HPE ZE E D JEHA RT AH UVISAVE HD2 CONFORMITY This system complies with the requirements of the EC Directive2004 108 EEC 2006 95 EEC and EN 61010 1 relating to Electro magnetic compatibility and low voltage The Electro magnetic susceptibility has been chosen at a level that gains proper operation in residential areas on business and light industrial premises and on small scale enterprises inside as well as outside of the buildings All places of operation are characterized by their connection to the public low voltage power supply system 128 128
31. ded by our service department is at the user s own risk and therefore automatically invalidates the warranty Tubes filters batteries and consumable products are not included in the warranty We reserve the right to decide where the faulty goods will be repaired in our workshop or elsewhere and whether or not the faulty part is to be replaced all other freight charges incurred being at the cost of the purchaser Returned goods will not be accepted for repair unless previous written authorisation is obtained from our service department A request for authorisation must be accompanied by an itemised list of products model numbers and the corresponding invoice numbers under which they were Originally shipped All returned goods should have a certificate of decontamination The Buyer must bear all costs and risks incurred during the transportation of the goods from their collection at UVITEC warehouse 126 128 i wet E T EE D tA urea In the case UVITEC incorporates some devices or equipment from another supplier in the manufacture of its products the extent and the duration of the warranty will be those conceded by the suppliers or sellers Manufacturer cannot be held responsible for any loss bodily injury or material accident incurred by any failure of this supply whatever the origin of this failure may be The responsibility of Manufacturer is strictly limited to its staff and to its own supplies In the case of dispute only the
32. e 8 A 1s is E ea ed Oa Lis ia IEL os E E Lis iim s Ee ut OAT E oar uisi itj ors te Beady MH Note You can display the results of a selection of lanes To proceed select the lane you want to display and click on Select To delete the display of some lanes select the lane and click on Unselect 100 128 i PRS Pee D s2e 1AKtAN Step 5 Quantify the volumes 1 Click on the Volume icon BWReference Default l F Exit E Print Pdefiniton EET Blgdefinition a Results Ans Volume M display Ny Autofit Help analysis Band A pop up window displays the following menu Volumes Siz led Profile type Complete Average Lane number Sm amp Volume calcul Threshold Scale of profile to display Minimum to masimum 2 For each lane the volume the height and the area are displayed in a table Cherri Capt MW RF analyor ix 1280 1 Prnt con G A gee PAi O aH Ay Aucen P ite ia a P LI 1359 f a LA 1 sted 5569 x5 I f 3 d Sa 5 10 n R 13 u 15 An 7 Ae 19 x a 2 rewchckd watse M1 Lane 1 lutte Hagrat dar Wieg fnd MSM MN 309 eee Bans me a0 wee Bans 04686 se 1s Bens 10680 mu 2084 Bane woul s3005 1 Gans Su ms o JO fang why 3 Lin fens 554 ats t Bando MIs uM e J ae Bsag 19 w323 i int Berd II 11997 a4 20 2 28 CSti ai a Basd 12 In 5399 27 t30 000 Bead 13 IIIN 833 I Berd 14 SI
33. e bands 70 128 r eo oe eae D a2t vrata 71 128 P SS Pee D JAEJHWAREAHN Step 2 Detect the bands Click on the Band detection icon Iam Lane o Band Marker w a Default voll a Exit fy Print Fanart Eb detection Ee values irq Results Ape volume MM Gispiay x Autofit Z Help ie oh A pop up window displays the following menu Band detection a Band detection Band edition Open the profile Linear cursor Close the profile Add or remove bands by clicking on the image gt Place the cursor at the chosen location and click The detection line is automatically added or removed You can edit the profile of one lane To proceed click on Profile Linear cursor or Profile Rectangular cursor 72 128 eS Pee ee F i ae JAEJWAREAH a i e E gt The linear cursor has the shape of an arrow _ gt The rectangular cursor has the shape of a square H You can add or remove bands by clicking on the image To proceed place the cursor at the chosen location and click The detection line is automatically added or removed 73 128 JOP el Pecne D a2 erates Step 3 Load or define the marker values Click on the Markers values icon Note The end of Step 2 Detect the bands opens automatically the Marker values pop up windows Note This function allows to assign the molecular weight markers values to th
34. e bands of the marker lane m Lane Band Marker Default Exit Pa Print oe IBIS detection PS values faa Results hn Volume il isplay N Autofit 2 Help i analysis A pop up window displays the following menu F Marker C amp S Select the lane corresponding to the molecular weight marker Marker lane number amp Define the marker s values by gt Loading existing values gt Creating new values gt Modifying existing values 1 Load existing values by clicking on the Load button A pop up window displays the following menu Look in O Molecular weight MW marker values Marker Files MK Select the molecular weight marker from the list and click on Open 74 128 L PPT Pee 32 strata 2 Create new values by clicking on the New button a i Me A pop up window displays the following menu Value editar Type your values lane by lane in a descending order The OK button validates your data Note For the size of the fragments and for the molecular weights the standard must be saved with the values in Kilobases and KiloDaltons only Example 1600 pb 1 6 Kpb Note A minimum of four values is necessary to validate the data Note if an automatic calculation with immediate application of the standard values is carried out it is not necessary to enter all the bands given by the manufacturer s specifications but
35. e requested size 4 Once the lanes are properly defined click on Detect the bands to trigger the detection Detect the bands 96 128 L EPS Pee D saat strsKrAd Step 2 Detect the bands 1 Click on the Band detection icon E Print em lane En Band A Reference Default 4 definition amp t2 detection t A pop up window displays the following menu a iy Results ime Volume i display w Autofit 2 Help E analysis Fr Lane definition o Lane direction Vertical lanes 0 Horizontal lanes Total lanes cancel i p 2 You can add or remove bands by clicking on the image gt Place the cursor at the chosen location and click The detection line is automatically added or removed 3 You can edit the profile of one lane To proceed click on Profile Linear cursor or Profile Rectangular cursor 97 128 i PPR SP eee D JAEVHWAREAN Ci BW a IY gt The linear cursor has the shape of an arrow gt The rectangular cursor has the shape of a square L 4 You can add or remove bands by clicking on the image To proceed place the cursor at the chosen location and click The detection line is automatically added or removed 98 128 Bf P ee Le LPNP sat ra wrtatt Step 3 Assign the RF values 1 You can assign the RF values to the detected bands To do this you must define an origin line value 0 and a front li
36. ecscsakceuiciiineacanina vet E E E 13 igloo lt iglo Melon UV Ar ADOD yT T arr a E E EEA ENEO EE E AE 13 ane a TVG OV a E E EE E E E EEE E A E E E E 15 UVISAVE 1D SOFTWARE INSTALLA ON creio insiens aa AEE ra 18 Software installation Preliminary SISOS mrirerisirerrorssreerirtisienoriivesiirtosnsroatinnorda toi rs torria si ari rendr EE Ee AEA iN 18 Minimum computer requirements for the UVISAVE 1D SOPTWOTEC oc ccscessscceesssseecsssececssssccesssseceetseeceesaeees 19 ON A T O r oA E EEA E E E T E EEEE EAE NRE 20 tjesi a a oi E E E 1 E E A A E A A A N A E 22 VTS Ve ABLE E aE E A E E A E E E S 22 SVS LENTOPERA TION cereiro eorr E E E A T E 23 waning ana Se CSS SS merian are a E T EE R E 23 E E a E EE EI ET E EE E A E aes 24 UVISOVE HD2 operating eNViONMENt eesssseesssseresesrreserrrrssrressrresssrrrserresssrenserresserenssrrerseresserreseeresserrererrereene 25 INVIA GE CAPTURE cirar n A E E AN 26 EE E AI AEI AA E E A EE 26 DE L a EE O EE EET A A A E T A EE 27 ELE EIA A E A E N E E EE A A E E E E E E E 30 A E E A E E E EA A E E E 31 E hee sei PA AEEA PE AAE EAA P AAE E A P ee AE EA N 32 EPI N EA T E AEE ENE A E N E A T A E E E E E ET 33 UE E AA a IEEE IAE EI EAE A EE T E EE T A E E EAE 34 KEA E E A E E EE EE E AEE AE E E EE EE E 35 OCS T N T a A EA E EE A EAN 36 SET UP MENU ccno O Ea 37 Setup men operating nvironmMeli siiisrsriticaisisirirniininir taiva Tinna ii tenana ANANAS EAN OTEA NAASE O ANAE aaia 37 BESE AE EEE a ATEI EAA EA PEE A E
37. ed on the screen 256 grey levels The easiest way to calculate the display image would be to translate the full grey scale each time an image is acquired the 4 096 values of the memory image corresponds to 256 values in the displayed image but in that case it won t be possible to visualize faint spots on a dark image UVIsave 1D offers you the possibility to select the grey level range to translate for the display image calculation All the grey levels under the Min value defined will be converted to 0 Black in the displayed image All the grey levels upper the Max Value defined will be set to 255 White in the displayed image The grey levels between those two limits will be converted in an intermediate grey level value following a linear rule For both values you can gt Enter it in the corresponding edit field gt Select the value by dragging and dropping the arrow gt Click on the Default button UVIlsave 1D calculates then the ideal values to be selected according to the parameters defined Display Gamma Gamma adjustment corrects an image by creating a new version of the original To create the new image the Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the following graph full scale d a ri gamma lt 1 Pixel Value New Image full scale i Pixel Value Original Image The above graph demonstrates the basic pri
38. ednattncmsaapeenaeties 63 EOC O RO Te A MAOT a A E E E E EA 65 PWC e is U T O U a E E AIE E AEN AEA 66 PAra E E E E E EN 68 Enhance Reset CENNANCEIMENL ccccccccssessccessessssccessessscceeseessecessesssecessseesecesseessseceseeusecesseeseeceessesseceeseeaes 68 MAGE ANALYSIS MOLECULAR WEIGHT s nssseesensenseseeoresreneeseoresreseeseoeesreneesrosesresressoeesrenrestonesresucatoeeorentearoeeoresteareeeoreneeaeenent 69 Accessing the QUICK image analysis MENU ccccccssssccsessccsssccessscccssescesssscssseecesescesescsssescseseecseaescseaeecssaeecsees 69 oaea DCT Me FS S Oi a A E E E A E A E 70 Nis Oly Poer DON err A N EE OE A T OAE OEA 72 Step 3 Load or GETING TNS marker VOIUES vscniinaasvcrreanndussisbabsonnmassisennigsiteaounaideneubenweabusssiiienceatbecmunessunsaanusandes 74 Sie Oe aE EE E E EE A A ee ne aan A A E E EA 77 Sepa QUAN TE VOUTE S eraan aE ERNA TO AEEA IEE AT O AEEA TEO A OEO AE S 78 TOTT TE U a E E E E E deacsenaspaioaccntes 80 ATO E E AT E E E E E EAE A E A A EE 83 Pe CSNY a E E E EAE E TAE E E E E A E EEN 83 FSC POT TOU ENO e ar E E E T E 84 IMAGE ANALYSIS COLONY COLIN EIN Giger sare ce as sirsie o E S N S N 85 Open an image and select the colony COUNTING MEenNnU esssessessssresseresoressresseressresseressressereseressrresere 85 POPC O COOR COUNT aee a E R 86 Manva caon FCO O oasiecerse aner settee asso stienetnae sn enna aAA EE ENE REO EEEE EEE NARAT EEEE NAE SAAE EARE 89 PRA FNS SIS a E A E E EE E EE
39. en will appear after the image is frozen 5d Gud gt gt gt gt Ha gt gt gt a gt gt gt gt 2 amp Print m Save Zoom 30 128 i DPS Pe D saat utara The UVISAVE HD2 system can calculate the necessary exposure time Auto exposure for a standard gel When you select the Auto Exposure option the system samples the light levels and uses the values to calculate the final exposure time AMPLITUDE EXPOSURE Note With long integration time a delay could be necessary before an image is displayed on the monitor The acquisition will stop automatically at the end of the exposure time At the end of the auto exposure process the image is automatically frozen The following screen will appear r f d ee gt gt 222 2222 2222 amp Print m Save Zoom 31 128 oh Ee AE ITER EE Clicking on Save will start the saving of the captured image The image is saved on the USB key drive according to the default image format To proceed click on the Save m key aims pii I 3 SAVING a wait a E E gt 22 2 PD 2222F gt gt gt amp Print mee Zoom Note The image file is named by the system The default image name has 8 characters The first 2 are IM The last 6 are a number ascending from 000001 to 999999 Note The default file name is incremented by one each time an image is saved Note The file extension varies according to the image
40. ents that are normally used with this instrument Use of the UV protective screen doesn t guarantee protection of the user from UV radiation The use of protective eyeglasses or mask and gloves is highly recommended 9 128 i HPE zE D 32 urea UVISAVE HD2 END USER LICENSE AGREEMENT Please read these instructions before installing and operating the UVisave system and software Please read carefully the following license agreement This document is a legal agreement between you and UVItec concerning the use of the enclosed software This agreement constitutes the complete agreement between you and UVItec By opening the software package or using the CD you are consenting to be bound by and are becoming a party to this agreement If you do not agree To all of the terms of this agreement do not open the package or use the CD Promptly return the CD including the written materials the binder and all other equipment containers to the dealer from whom you purchased them License Grant The package contains software Software and related explanatory written materials Documentation Software includes any upgrades modified versions updates additions and copies of the Software You means the person or company who is being licensed to use the Software or Documentation We and us means UVItec SAS We hereby grant you a nonexclusive limited license to use one copy of the Software on any single computer provided the
41. er Yes to the question and programs are removed gt Click on OK to finish de installation 22 128 ee i oe oe a sat saat UVISAVE HD2 SYSTEM OPERATION i Foldallave Kolelo EXT AATE WARNING The transilluminator is used for the fluorescence applications WARNING The use of the UVISAVE HD2 involves ultraviolet UV illumination Proper precautions must be taken to avoid eye and skin exposure to the UV light This instrument is meant for use only by specialised personnel that know the health risks associated with UV radiation and the chemicals that are normally used with this instrument WARNING The operator should wear appropriate safety glasses or a protective mask and gloves UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 Caution Switch off the transilluminator when gel is not present on the UV filter If the filter is too hot it will damage your electrophoresis gel 23 128 L PPT Pee IAEA Main switch The UVISAVE HD2 main switch is located at rear side USB camera and printer connections o e e s Port 1 Main switch and Power supply Connection gt Switch on the main switch before to use the system 24 128 Pee a AE JETA th HAH UVisave HD2 operating environment Power the UVISAVE HD2 using the ON OFF switch of the control panel ON O
42. er the Software to a single hard disk provided you keep the original solely for backup or archival purposes and provided that the original copyright notice and or other legend is reproduced on each copy Terms and Termination This license agreement takes effect upon your use of the software and remains effective until terminated You may terminate it at any time by destroying all copies of the Software and Documentation in your possession It will also automatically terminate if you fail to comply with any term or condition of this license agreement You agree on termination of this license to either return to us or destroy all copies of the Software and Documentation in your possession Confidentiality The Software contains trade secrets and proprietary know how that belong to us and it is being made available to you in strict confidence Any use or disclosure of the software or of its algorithms protocols or interfaces other than in strict accordance with this license agreement may be actionable as a violation of our trade secret rights General Provisions e This written license agreement is the exclusive agreement between you and us concerning the Software and Documentation and supersedes any and all prior oral or written agreements negotiations or other dealings between us concerning the Software e This license agreement may be modified only by a written agreement signed by you and us that specifically supersedes this agreement e
43. file format gt For TIFF the extension is TIF 32 128 12D PES Pee a2 strana To print press the Print key Prine N The image is printed on the default printer if defined POUT EI EEEH EI _ _ aa eamus Lome e e a o er gt gt gt a gt gt D gt Ha DDD amp Print m Save Zoom Note a printer message can appear on the display in the case of lack of ink or paper In such case the message should disappear after few seconds If not please press any touch from the panel and the message will disappear after few seconds 33 128 a if Per Pa 32ers rte You can zoom in the image when pressing the Zoom key Then with the up down left right arrows you can navigate with the image The zoom view displays the Amplitude bar graph as well as the minimum and maximum grey level for the image Press the Back key to return to the previous menu 34 128 Poo ee a E i i JAE Hae Next Back The Next and Back keys help you to navigate within the menu ee a M s _ e ee s S D gt gt gt SD gt gt gt SEED gt gt gt SP ae B Print Save Zoom Gack From the back button you access the Live Exposure and Auto keys 35 128 oo ee E r JAE ITAA AM Focus point adjustment All cameras require a focus point adjustment In order to proceed you have to zoom to the maximum on
44. focal distance All other image acquisition parameters Real time acquisition or integration time positive or negative image are recorded Treatments on frozen images contrast luminosity inversion are also recorded Crop images The Crop images feature allows you to combine two or more images to create a composite image that shows a portion of each image The crop option is used primarily in fluorescence imaging when specimens have been stained with more than one dye Define the target image and the sources 113 128 JAE ur ATA Select images axe Target image Browse _ Source 1 Browse Source 2 Source 3 Browse Annuler L 2 Define in the sources image the area you want to be cropped in the target image gt 0 4095 4 Image a dynamic Intensity Min Max o Minimum Maximum Gamma P 1 00 E DISPLAY _ Crop Images a 3 Click on crop images to obtain the target image 114 128 a E y EE D JIEJWARTAH Merge images The Merge images function is used to combine by superposition two or more monochrome images into a single 24 bit coloured composite image 1 Define the target image and the sources Select images mn Target image Browse Source 1 Browse Source 2 Browse _ Source 3 Browse _Amnuler 2 Define in the pseudo colours in the sources image gt 4095 AAS v Y Image
45. g menu in which you can adjust the colours of the image Color palette selection m Definition of a new point Selected point _ a Self made v Load design Default design OK Cancel The image is then displayed with the default pseudo colours settings For instance the image could be as followed Colours adjustments For the bicolour selection click on the arrow to define the value of the colour you want to modify While keeping the mouse button pressed move the arrow to its new value Release the mouse button when value is satisfactory the image is automatically updated You can repeat these operations as many times as necessary for all pseudo colours 66 128 Jf0P 82 Pogo JAE PAs Add or remove a colour Click on Add a point to add a colour on the pseudo colours list Select the colour from the Add a point palette Automatic More Colors Select the point to remove and click on Remove point to remove a colour from the pseudo colours list Default predefined and user defined palette design The UVIsave 1D software has several predefined palette designs Select your palette design from the design list Self made Self made Ascending grey levels Red palette Green Fluorescent protein You can also save and load your own palette design Define the set of colours you want to apply and click on Save to save the palette design Click on Load to open your palette de
46. gt Click on Print to print as previewed Click on Close to close the Print preview and to go back to the main menu 118 128 L PPT Pee E JAEJWARTAH 3 The Print Setup allows you to choose a printer and to configure the printing To proceed select File Print Setup from the Menu bar Close EJ Save Print Setup 1 IMO00012 2C WLIMAGES JM000001 Print Setup EPSON Stylus C70 Series vi Ready EPSON Stylus C70 Series USB001 Comment Paper Orientation Size A4 210x 297 mm Portrait Source Bac feuille feuille Landscape Select a printer Click on Properties to modify the default setting of the printer if necessary Select the paper size and source select the orientation Click on OK to validate your options Note After you have installed and setup your printer the procedure for setting up and configuring a printer is the same as in other Windows program Printing on the video printer if applicable Optional 1 To print on the video printer click on the Print on the video printer icon Note The video printer needs to be connected to your computer Note The video motorized zoom PCI board need to be installed in your computer 119 128 L PPT Pee a D saat vrata Crop Defining the area to be saved 1 Click on the Area to save icon 2 A default area is displayed Click on the tags surrounding the area to modify its size Drag and drop the area t
47. gt Select a printer Click on Properties to modify the default setting of the printer if necessary gt Select the number of copies Click on OK to validate your options Note You can also access the Print menu from the Menu bar File Print 3 The Print preview displays a preview of the image as it will be printed To proceed select File Print Preview from the Menu bar Close 1 1M000012 2C WLIMAGESIMO000001 90 128 LDPE Pee JAE Aas A pop up window displays the print preview Selection of window mage view C 9 gt Select the window to be previewed A pop up window displays the print preview gt Click on Print to print as previewed gt Click on Close to close the Print preview and to go back to the main menu 4 The Print Setup allows you to choose a printer and to configure the printing To proceed select File Print Setup from the Menu bar Print Print Setup R 1IMO00012 2C VLUMAGESIMO00001 Ext A pop up window displays the print setup menu 91 128 ao ee E sere D JAEJWAREA Print Setup ame OES Ready EPSON Stylus C70 Series USBOO1 Orientation A4 210x 297 mm Portrait rce Bac feuille feuille x O Landscape gt Select a printer gt Click on Properties to modify the default setting of the printer if necessary gt Select the paper size and source select the orientation Click on OK to validate your opti
48. he display image would be to translate the full grey scale each time an image is acquired the 4 096 values of the memory 83 128 JSP etPecee sat strantan image corresponds to 256 values in the displayed image but in that case it won t be possible to visualize faint spots on a dark image UVIsave 1D offers you the possibility to select the grey level range to translate for the display image calculation All the grey levels under the Min value defined will be converted to 0 Black in the displayed image All the grey levels upper the Max Value defined will be set to 255 White in the displayed image The grey levels between those two limits will be converted in an intermediate grey level value following a linear rule For both values you can gt Enter it in the corresponding edit field gt Select the value by dragging and dropping the arrow gt Click on the Default button UVIlsave 1D calculates then the ideal values to be selected according to the parameters defined Returning to the main menu e ext To return to the main menu click on the Return to the main menu icon analysis HERE EHTE 84 128 TTF E P re F Aa uaa ON IMAGE ANALYSIS COLONY COUNTING Open an image and select the colony counting menu Note the colony counting is designed for 8 bit image displayed image 1 Click on the Open icon A pop up window displays the following menu L
49. ic modify the greyscale selection to enhance the image display Ea AutoScale Minimum Maximum 14337 3 Gamma iad Saturation JE 1 00 DISPLAY The acquired images are for instance 12 bit ones ranging from 0 up to 4 095 grey levels Windows can only display 8 bit images 256 grey levels Due to this limitation for each acquired image the UVIsave 1D software handles two images gt a memory image corresponding to the image acquired 4 096 grey levels gt a display image corresponding to the image displayed on the screen 256 grey levels The easiest way to calculate the display image would be to translate the full grey scale each time an image is acquired the 4 096 values of the memory image corresponds to 256 values in the displayed image but in that case it won t be possible to visualize faint spots on a dark image UVIsave 1D offers you the possibility to select the grey level range to translate for the display image calculation All the grey levels under the Min value defined will be converted to 0 Black in the displayed image All the grey levels upper the Max Value defined will be set to 255 White in the displayed image The grey levels between those two limits will be converted in an intermediate grey level value following a linear rule For both values you can gt Enter it in the corresponding edit field gt Select the value by dragging and dropping the arro
50. image displayed on the screen 256 grey levels The easiest way to calculate the display image would be to translate the full grey scale each time an image is acquired the 4 096 values of the memory image corresponds to 256 values in the displayed image but in that case it won t be possible to visualize faint spots on a dark image UVIsave 1D offers you the possibility to select the grey level range to translate for the display image calculation All the grey levels under the Min value defined will be converted to 0 Black in the displayed image All the grey levels upper the Max Value defined will be set to 255 White in the displayed image The grey levels between those two limits will be converted in an intermediate grey level value following a linear rule For both values you can gt Enter it in the corresponding edit field gt Select the value by dragging and dropping the arrow gt Click on the Default button UVIsave 1D calculates then the ideal values to be selected according to the parameters defined 93 128 eRe Pee JAE HAR Returning to the image main menu e Ei 1 To return to the main menu click on the image capture icon analysis E Chemi Capt Colony counting COLONY2XTIF b a pj File Coley counting Window Hep A new menu appears with the main menu task bar functions 94 128 TTILE F TEUA RAD IMAGE ANALYSIS RF 1 Click on the Open icon A
51. k on the Autorun exe file to start the installation of the software The UVisave 1D Setup window will appear welcoming you to the Install Wizard for UVIsave 1D Setup Install Open user software manual Browse Get Acrobat Visit UVITEC CD Rom Reader c website UVITEC C am bridge gt Please click on the Install software icon Step 2 A pop up windows displays the following welcome screen Welcome to the BioCapt Setup pogam This peogiam wal install BioCapt on your computer It it sironghy recommended that you axit all Windows programs belare running thes Setup program ects Daoa a tah cence AON Gree ROSE FSI uae RAT Click Next to continue with the Setup progr jei This program is protected by copyright law and intemational Unauthorized reproduction or distribution of thes program or any portion of it oY eee ee on and wil be prosecuted to the maximum exter possible under Law Cie er comet Click on the Next button to continue Select the destination directory The default destination directory is displayed C Program Files UVIsave 1D 20 128 12 PPS Pe Ee JAE VHA Choose Destmation Location Setup wil instal BioCapt in the followang directory To install to this directory cack Next To install to a dilferert directory chck Browse and select another directory You can choose not to install BioCapt by chcking Cancel to at Setup Destination Directory ceca lt a he L
52. llation options Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CO AOM E 4Canon E O Don t search will choose the driver to install Choose this option to select the device driver from a lish Windows does not guarantee that the driver you choose will be the best match for your hardware Select the USB key drive which contain the printer driver Then click on Next The following window will be displayed Found New Hardware Wizard Please wait while the wizard searches op CanoniP 3000 Cancel Then the following window will be displayed L PPT Pee tat straw 47 128 Lf PES Pe 2 ee JAE VPA ee at Found New Hardware Wizard Completing the Found New wy Hardware Wizard The wizard has finished installing the software for _ Canon PISMA iPs000 Click Finish to close the wizard Back Cancel Click on finish The printer is then installed Please switch off the UVISAVE HD2 system and re start 48 128 12D PRS Pee D raat vtrawertat About The About folder summarizes the information concerning your UVISAVE and its set up It gives you access to the following information gt Systems name amp version gt Camera state gt Printer state To access About menu press the Set up me
53. llowing diagram illustrates the bar E vooo orni e cor J FEeronein J EN Prone ou Z rer 2 3 4 5 6 7 8 Open an image Save an image Print on the default printer if connected Select the image area to be saved Save the user setting Open the user setting Open the Help file on a specific function 56 128 a L P aL Pee 32 sranrAH Navigating the toolbar The UVIsave 1D software offers 2 modes gt Image analysis modes gt Controls and parameters mode Each mode is gathered in a specific folder _ J a Image analysis TO Controls Parameters You can select the application by clicking on the specific folder The status bar The status bar allows you to Monitor the image dynamic Image a s aynamic Intensity Min Max 440 16383 Modify the display of the image autoscale inverse saturation gamma and greyscale selection to enhance the image display On AutoScale Minimum Maximum 14337 Gamma ij Saturation EPE Enhance the image DISPLAY ENHANCE 57 128 roe PS Pes D JAE HWARIAH ON THE STATUS BAR Introduction The status bar allows you to gt Monitor the image dynamic Image i g aynamic Intensity Min Max 16383 IMAGE DYNAMIC Modify the display of the image autoscale inverse saturation gamma and greyscale selection to enhance the image display On Auto Scale Minimum Maximum 14337 Gamma Cy Sat
54. m the unit before performing any disassembly or repair to the system WARNING The use of the UVISAVE HD2 together with a transilluminator darkroom involves ultraviolet UV illumination Proper precautions must be taken to avoid eye and skin exposure to the UV light This instrument is meant for use only by specialized personnel that know the health risks associated with UV radiation and the chemicals that are normally used with this instrument WARNING The operator should wear appropriate safety glasses or a protective mask and gloves UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 WARNING The UVISAVE HD2 system should be located away from water solvents or a corrosive material on a bench top that is dry and stable The system should be placed away from interfering electrical signals and magnetic fields A dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your laboratory 14 128 no ee a F ian pi D s2t urna Note The UVISAVE 1D software runs only with Windows XP SP2 or upper 32 bit or Windows Vista SP1 or upper 82 bit operating system You must ensure Windows is installed on your computer before performing the UVIsave 1D installation Installing the hardware Place the UVISAVE HD2 on a level working surface Stand by Port 4 Fig
55. mage name you want to load Note the catalogue function allows a preview of the images located in the selected directory To proceed select one image of the directory on click on Catalogue 116 128 p i Pes Pea f JAE VHA Saving an image location 1 Click on the Save icon 2 A pop up window displays the following menu Save in Molecular weight 0 2 em l igducchini r Igrs 30min Igrs 30min 8bit i ig wonkygel IM000004 imo00006 1M000011 1IM000012 i 1M000013 1M000020 imo00033 im000043 imoo00ss im000059 im000073 i im000092 IM00000111 i im000124 im000135 IM00000311 i imo00055555 IM0000 12222222 i ircsSmin 30sec irsc10min e File name 1M000002 Save Save as type Tagged Image File Format TIF v Save As 3 Browse to specify the image directory in 3 Molecular weight l GB My Documents B Mes images 1M00000111 i im000124 im000135 This function saves a previously unsaved image to a new file or update the changes to an existing image file or save an image to a new file or file m N ig My Computer a 3 Floppy A amp Local Disk C CD Drive D Sa IOMEGAMINI E G Shared Documents G My Documents a My Network Places gt Unused Desktop Shortcuts Save as type Tagged Image File Format
56. mears and artefacts can be eliminated with this coefficient which calculates how stretched the colony is E W mini width 0 lt E lt 1 L maxi length Note You can display the colony number on the image by clicking on the Display colony number Display colony number 5 You can exit the colony counting function by clicking on the OK or cancel button 88 128 i DPS Pe L E JAE JHAR Manual colony counting Note the colony counting is designed for 8 bit image displayed image counting 1 Click on the Manual colony counting icon from the tool bar Manual counting 2 To count a colony click on it with gt the left mouse button to count it as a green gt the right mouse button to count is as a red colony Note To cancel a counted colony click once again on it with the appropriate mouse button Note To reset the counting press the Reset button 3 You can exit the colony counting function by clicking on the Ok or cancel button 89 128 ono oe ei Jeni JAEJA Printing the results 1 Click on the Print icon A pop up window displays the following menu Selection of window E C Coa O Select the window to be printed 2 Click on OK to validate your choice A pop up window displays the following menu EPSON Stylus C70 Series v Properties Ready EPSON Stylus C70 Series USBO01 Comment Print range All
57. nciples of gamma adjustment 61 128 Jerez Peegue D j2t sranztan Black pixel value 0 remains black at all gamma values White pixel value full scale remains white at all gamma values Gamma values greater than one lift the darker areas of the original image into the brighter areas of the new image A gamma curve is smooth there are no unexpected jumps or cut offs This means that when viewing a gamma adjusted image you will be able to see the details intensity differences in both the black and white areas of the image When the bright areas of these types of images are correctly exposed the darker areas can be so dark that they are in effect invisible Gamma Adjust can remedy this problem The gamma adjustment results in a better display of detail by lightening the darker areas without burning out bright areas or lightening black areas Enhance Date Click on the Insert date and time icon to insert the time and the date on the image With kal the date and time are incrusted in white TUESDAY APAI 27 2004 7 05 AM With Kal the date and time are incrusted In black Note click once again on the activated icon to remove the incrusted text 62 128 Cee oe ee n i Pi JAEJHA RAAH Enhance Brightness Contrast You can adjust the brightness to lighten or darken an entire image To proceed click on the Brightness icon A pop up window displays the following menu Brightness c
58. nd enhancement menu contains enhance gt _ the image enhancement functions ment gt the annotation functions the Good Laboratory Practice GLP functions The following image illustrates the Text and enhancement menu a Inverse the image Adjust brightness C Adjust contrast Rotate image SL Horizontal mirror Vertical mirror Undo adjustments Add test and symbols GLP view Export GLP file BET Ee ae mage cropping Image merging Image overlaping Controls The Controls and parameters function contains and gt the Autoscale function gt UVlIsave 1D parameters paramete rs gt the language selection The following image illustrates the Advanced functions menu Controls amp parameters Auto scale Photo Capt parameters Language d 55 128 Window menu ee E LRF D JEWISH The Window menu contains gt the window management function the list of the opened images The following image illustrates the Window menu Cascade Tile Arrange icons 2 IMO00001 TIF The Help menu contains gt the help index gt the contextual help gt the version details of UVIsave 1D The following image illustrates the Help menu Index Using help ea About Navigating the function folder The function folder to frequently used menu items It appears underneath the Menu bar and contains main tool fe 2 a 4 5 6 7 file management and user profile functions The fo
59. ne value 1 Click on the Reference definition icon Paat Print Lane i Band Reference ER Default l Exit 1 definition n E e Results An Volume 7 display N Autofit Z Help analysis t Note The end of Step 2 Detect the bands opens automatically the Marker values pop up windows A pop up window is displayed input RF original and final values Eg Original AF value Final AF value The select the value for the origin and the value the end Then validate by clicking on OK An origin and a front line are displayed on the image MM R F analyse 240 22 Click on the first line Kept pressed the left mouse button and move it to the location for the origin line Then release the button Click on the second line and move it to the location for the migration front Then release the button The R F values are assigned to the bands 99 128 A smPre one D JAERI Step 4 Get the results 1 Click on the Results icon PoF Cane ti Band Reference EA Default l Exit Eh Print Po EE detection M Wy Results volume o a QZ Help ee on A pop up window displays the following menu Results f m fx Lane num cam Select Select all Reset x j The results are automatically displayed in a table a LE S Autofit t iL 11 Fi LF i s 4 i a a a a x I Libras Lies 3 j Lares 4 Lane 5 J liari h Lara 7 Lan
60. noianeuaesatesanarsnesancenens 110 ACCESSING TNE controls amp parameters FOILED oe eeesccssssccssssccsssccessscesssccsssescesssscesseecseseecssseecseaeecseaeecseaeeens 110 UvVisavye ID 10 0109 23 a1 perenne rere rene enor ene eee en ar ee Ser ene er 110 GOGG laboro Sr errereen in Ei NEAR AE iain 113 OSS oa canes atic AE E E E E EA EAEE E E E EEE E 113 ATIT GSS A A E E A O E A E N EE A A TONE 115 TOOLB ARAN IDE TAINS pa E E EE E T A E E A E AE N E EAA 116 PET I Te e a E E A N A AA E N EA A N 116 SS a Sko nE D a e AE I E E A E A EAT ga oa IRET E E E E T E eaneeetes 117 PPT a E E E E EE E E A O E E E A E E E 118 Printing on the video printer if applicable Optional cecescccsessccssscccsssscessccesssscessescsssescsssesessasecseaees 119 Chop Denng Me wed To De saV san a A EEA E E E E eee mene ae 120 DVIS TS SS T S E EE E EAE E EAA O EE T 121 LOGAR FSS SINOS arene n E ET E E E EET EE EE E 122 Unga Teco xuda NO ser saraa aa A E N A OE EO 122 MAINTENANCE scrnornnrirrn N sie ca sa veuiald rons eabne nena aaoinpene eoanameenauaniers 123 CT OO eea EA E E EAE AE AAAA E ENE AEE 123 WORO O a N E E E EE O AEE EAE N E EA EEE EE A 123 PTY IOT O A A A IA A E AEA A E E A A A AN 123 TECHNICACINEORMA TON cresian E A A T E NO 124 PC Ol SOS T r E NE A EEA A A 124 DECREE DE E E M E E E E E EE E E AEE E E are 124 SPARE PARTS srn EE E E se Gee nueseseueuenescasseeseseusesses 125 eS 29 p E A E E AT A A A A A E E AT 125 WARRANTY siima a E E E NE A A E 126 C
61. nu key and select the About option UVISave version 15 01b UVitec Cambridge Ltd Copyright C 2009 2010 www uvitec co uk Camera connected Keyboard not connected Default printer HP LaserJet 5000 Series PCL6 Ready lf a USB key drive is connected you can export the UVISAVE information to the USB key by creating a text file when pressing the Export key UVISave version 15 01b UVitec Cambridge Ltd Copyright C 2009 2010 www uvitec co uk Camera connected Keyboard not connected Default printer HP LaserJet 5000 Series PCL6 Ready OK i Export l 49 128 TTILE P Lf PES D saat ura Firmware update If suggested by UVItec the UVISAVE firmware can be updated using a special program and a zip file provided by UVItec This program and this zip file need to be copied on a USB key drive Insert the drive in the UVISAVE and access the About function through the Set Up The About folder summarizes the information concerning your UVISAVE and its set up It gives you access to the following information gt Systems name amp version gt gt Camera state gt Printer state To access About menu press the Set up menu key and select the About option The following menu will appear UVISave version 15 01b UVitec Cambridge Ltd Copyright C 2009 2010 www uvitec co uk Camera connected Keyboard not connected Default printer HP LaserJet 5000 Series PCL6 Ready Select the update key The system will
62. o modify its position Note To erase a previously defined area click once again on the function 3 Click on the Save as icon A pop up window displays the following menu Save As Save in 3 Molecular weight Ge gt fale i igducchini 1M000013 i 1M00000111 i Igrs 30min 1M000020 i im000124 Igrs 30min 8bit im000033 im000135 ig wonkygel i im000043 i 1M00000311 1M000004 i imooo0ss i imo00055555 i im000006 i im000059 i IM0000 12222222 i 1M000011 i imo00073 l ircsSmin 30sec i 1M000012 i imo00092 l irsc10min lt File name m000002 Save as type Tagged Image File Format TIF vi Cancel 4 Browse to specify the image directory Save As q 1M00000111 i g hag im000124 O i im000135 I My Computer IM00000311 H 3 Floppy A i imo00055555 lt Local Disk C i IM000012222222 44 CD Drive D ircsSmin 30sec lt IOMEGAMINI E i irsc10min jay Shared Documents Gay My Documents File name i My Network Places gt Unused Desktop Shortcuts Save as type Tagged Image File Format TIF 120 128 Save in Molecular weight i RPER ES Pee JAE JHAR 5 Enter the desired file name select a file extension and validate O 2 pE igducchini Igrs 30min 1M000004 i im000006 1M000011 1M000012 IM000013 i IM000
63. only those which are commonly found on the lanes of the gel 3 Modify existing values by clicking on the Modify button A pop up window displays the following menu on which you can modify the marker values Value editor 75 128 i PES Pee JAE VPA ot Migration curve Note The migration curve allows to check the detection of value application errors distortion errors bad separation between the bands or the quality of the standard itself Note To delete the wrong lane s data you can either place the cursor arrow on the wrong value itself and click on it or go for the manual assignment Note The displayed migration curve is of the cubic spline type and must then include a minimum of 4 values Assign manually the marker values to the lane by selecting the appropriate option Assignment O Automatic Manual The markers values and the profile are displayed in the same window PHBE Click successively on the value inside the rectangle then on the band itself A link between the value and the lane is displayed Place now the arrow on the second value and use the same method up to the last value Note A minimum of four values is necessary to validate the data Click on OK to exit the marker values function 76 128 L PPT Pee JAE HARD Step 4 Get the results Click on the Results icon Lane Band A Marker i Default Exit iiey 1 ane 2 detection 3 value
64. ons Note After you have installed and setup your printer the procedure for setting up and configuring a printer is the same as in other Windows program Autofit Click on the Autofit to resize the image to fit the size of the monitor The full resolution of the acquired may be larger than the screen resolution The navigation requires the Windows scroll bar The Autofit allows you to view the whole image regardless of the window size Typically reducing the size of a window also cuts off part of the image The Fit to Window option solves this problem by resizing the image so that it is always the same size as the window The Autofit feature proportions the display of the image to the screen resolution Autofit no scroll bar No Autofit scroll bar 92 128 a ASR PS LD Pee eS ARE Urata Default display Default display The Default display windows allows to gt monitor the image dynamic gt modify the greyscale selection to enhance the image display Minimum Maximum 14337 Gamma Cia Saturation B ioo DISPLAY The acquired images are for instance 12 bit ones ranging from 0 up to 4 095 grey levels Windows can only display 8 bit images 256 grey levels Due to this limitation for each acquired image the UVIsave 1D software handles two images gt a memory image corresponding to the image acquired 4 096 grey levels gt a display image corresponding to the
65. ontrol Specify the percentage to add or remove from the image gt Increase the brightness by defining a value above 50 gt Decrease the brightness by defining a value inferior to 50 gt The image is automatically updated Before After i e brightness 40 You can adjust the contrast to exaggerate or subdue the difference between the bright and the dark area of an entire image To proceed click on the Contrast icon A pop up window displays the following menu Contrast control Adjust contrast 4 Laat Lo cancel O Specify the percentage to add or remove from the image gt Increase the contrast by defining a value above 50 gt Decrease the contrast by defining a value inferior to 50 gt The image is automatically updated 63 128 12 PPE SP eee JAE HA Before After i e contrast 70 64 128 i PES Pee JAE VHA AH Enhance Rotate Mirroring Click on the Rotate icon to rotate right the image The image is rotated clockwise in 90 increments Before Before Click on the Vertical mirroring icon to flip the image from right to left Before 65 128 Li PEL Paoee Aae uaa Enhance Pseudo colours The pseudo colours can display different types or levels of fluorescence in an image It replaces the original grey levels of the image by another palette colour To proceed click on the Pseudo colour icon A pop up window displays the followin
66. ooe You can select another directory by clicking on the Browse button and select the directory on which you went to set the UVIsave 1D software Choose Folder Directories E gt Program Files B Vilber Lourmat Cg BiolD C ChemiCapt C3 ChemiCapt3000 Network Click on the Next button to continue The program files are installed in the specified directory Once the software is installed the system is updated This can take on few minutes depending on the computer speed Setup Complete Click Finish to complete Setup As soon as this process is over please reboot the computer when the installation software proposes it UVIsoft group consists of the icon of the software and of the un install program Click twice on the program icon to run it The most current way to launch the software is to click on the Windows Start button then on All programs and then on UVIsoft group To open the UVIsave 1D software click on UVisave 1D from the UVIsoft group 21 128 Bt Peele r ice D JIEJHARIAH Upgrading the software If you want to upgrade the software to this version proceed as described in the first Installation chapter UVisave 1D un install You may want to un install the software from your hard disk The uninstUVISAVE 1D program will do it for you gt Click on Start button gt Go to Programs select UVIsoft and click on uninstUVIisave 1D Answ
67. ook in C Molecular weight i imo00033 00000111 l imooo043 im000124 i im000135 imoo00s9 1Mo0000311 lm imooo073 i imooo0ss555 imooo092 100001222 4 iiil ta Files of type TIFF Files TIF ka Cancel Catalogue 2 Browse to specify the image directory 3 Double click on the image name you want to load Note the catalogue function allows a preview of the images to load in the selected directory To proceed select one image of the directory on click on Catalogue 4 Select the Image analysis folder From the Image analysis folder select the Colony counting option a _ Image analysis Distance calculation RF Colony counting 85 128 i DPS Pee ee IAEA hana Automatic colony counting Note the colony counting is designed for 8 bit image displayed image counting 1 Click on the Automatic colony counting icon A pop up window appears with the colony counting functions Colony Counting Detection Area of analysis O Circe Rectangle O Ary CI Square Colony detection parameters 100 lt Green lt 120 150 lt Red lt 17 C Display colony number 86 128 F LN PE HEEE D ta strata 2 Select your area of analysis counting Area of analysis Circle Rectangle Ary Square There are 3 kinds of area of analysis Circle and rectangle Click on the corresponding option to select it The
68. op number the brighter the image will be For enhanced gel imaging we suggest to position the diaphragm ring around aperture 4 36 128 BIPeeee i i tat straw UVISAVE HD2 SET UP MENU Set up menu operating environment You can access a set up menu from which you will be able to manage the following functions Display the saturation Add a grid Stamp the date amp time Open or delete an image Modify the date and time Unlock the system and add a device such as a printer Check the system status and version D Dy et up All the functions of the set up menu are described in details in the Setup Menu chapter of this manual To access the Set up menu press the Set up menu key 37 128 Bl Pee ee P i Pe SE rat vrata Advanced set up Set date and time Add a device i e a printer About D 2 Open an image Delete an image Stamp date amp time OFF Add a grid OFF Display the saturation OFF r g g g g g 38 128 LDPE SP eee JAE STAR Display the saturation Saturation occurs if the amount of light collected by the camera exceeds its capacity In such case the image is over exposed and the integration time should be reduced A saturated image is inappropriate for image quantification with image analysis software The saturation option allows you to visualize the saturated pixels in order to avoid flattening the peaks To proceed press the
69. orizontal line to display the band contours to quantity 78 128 P Lf PD Pee ee D JAEJWARTAH gt Click on the left button of the mouse to validate the values are directly displayed gt The defined threshold is automatically applied to all lanes The results are recalculated taking into account the threshold n nt oe A f A p ilitata 4 a o a 1 ma g 11 13 15 3 79 128 GSP eS ee r Pi D a2 stranan Printing the results 1 Click on the Print icon A pop up window displays the following menu Selection of window Profile or marker view Array of result view gt Select the window to be printed gt Window 1 refers to the analysed image gt Window 2 refers to the lane profile or the molecular weight markers view gt Windows 3 refers to the result table 2 Click on OK to validate your choice A pop up window displays the following menu EPSON Stylus C70 Series v Ready EPSON Stylus C70 Series USB001 __ Print to file Print range Copies Al Number of copies OPGELE gt Select a printer Click on Properties to modify the default setting of the printer if necessary gt Select the number of copies Click on OK to validate your options Note You can also access the Print menu from the Menu bar File Print The Print preview displays a preview of the image as it will be printed To proceed select File Print Preview
70. pop up window displays the following menu Look in 9 Molecular weight Imoo0004 imoo0033 i IMOO000111 imo00006 imO00043 im000124 i li imo00135 im000059 IMO0000311 im000073 lm imoo0055555 im000092 lw mo0001222 i Files of type TIFF Files TIF Catalogue 2 Browse to specify the image directory 3 Double click on the image name you want to load Note the catalogue function allows a preview of the images loaded in the selected directory To proceed select one image of the directory on click on Catalogue 4 Select the Image analysis folder From the Image analysis folder select the Colony counting option D _ Image analysis Q ick eae am FEA 3 Molecular weight 3 E Colony counting 95 128 L PPT Pee ee s32t 1arAn Step 1 Define the number of lanes 1 Click on the Lane definition A Print Lane Band Reference 3 definition Oy Results In Volume iainih N Autofit Help ra Exit 1 definition amp f2 detection analysis t 2 Choose the direction of the lanes from gt horizontal gt or vertical Lane direction Vertical lanes Horizontal lanes 3 Select the number of lanes Total lanes So gt gt On the image click and drag to define the analysis area and to overlap the lanes gt You can adjust the size of the area by clicking on the tags surrounding the area and drag the selected border to th
71. rument not performed by UVITEC or by an authorized agent All uses opposed to the notification specified in this technical instruction or all misuses affect the goods and bodies security fastened by this device and can damage the instrument lt is mandatory to connect the UVISAVE HD2 to an appropriate AC voltage outlet that is properly grounded and protected by a circuit breaker Connecting to ground constitutes an obligatory protection lt is mandatory to power down the system and to disconnect the AC main from the unit before performing any disassembly or repair the instrument The system must be unplugged from the AC voltage outlet if it is not intended to use it before a long time Disconnect the power cord by pulling the plug Never pull on the cord itself Ensure that all the ventilation opening systems are not obstructed The obstruction of the air admission grids may affect the performance of the system and cause operational failure To prevent fire or shock hazard do no expose the unit to rain or moisture The use of accessories not supplied by UVITEC can damage the system or create safety hazard Do not use the UVISAVE HD2 in dangerous atmosphere or with dangerous materials for which the UVISAVE HD2 has not been designed for The power cable of the camera is expensive and very fragile because of the thin conducting pin Before plugging or unplugging the cable ensure the unit is off and disconnect the UVISAVE HD2 from
72. s E4 poen An en display N ies p Help analysis Note The end of Step 3 Marker values opens automatically the Results pop up windows A pop up window displays the following menu Results f m Eg Lane num Note You can display the results of a selection of lanes To proceed select the lane you want to display and click on Select To delete the display of some lanes select the lane and click on Unselect 77 128 i DPS Pe ee D JEWISH Step 5 Quantify the volumes Click on the Volume icon ee ee a i onir i Exit Eh Print eo EP detection ry values ira Results PARE Volume THM Gispiay Ny Autofit 2 Help ES analysis A pop up window displays the following menu Volumes A Profile type Complete Average Lane number lt W Volume calcul Threshold j For each lane the volume the height and the area are displayed in a table Dlan LAW IL dnak Euchar SE GEG Ni ral Elk i i P t H I HEEE gt The volume is the sum of all the intensities included in the defined area window separation gt The height is the maximum intensity gt The area is defined for each peak by the width of the window and the separation lines You can define a quantification threshold To proceed gt Click on the Threshold button The threshold allows to distinguish the bands from smears on the lane gt Move upwards on the screen the h
73. saturation key The saturated pixels appear in red on the display MAE 1 EHAN EHAU AIE 1a saat ro f lt 222 gt D222 F 2222 2222 22227 Sr Live kr E Freeze Auto Note A saturated image creates quantification error when studied by image analysis software Note To un activate the saturation option press the saturation key one more time Note The image saturation is also indicated by a red line in the Amplitude graph 39 128 JP oo ae oe Jerez satura Add a grid Add a grid With the grid option you can display a grid on the screen to adjust your gel according to horizontal and vertical axis To proceed press the Add a grid key The grid appears as below with the Live mode T a Some quan pai em n LIVE j pampe ii pot i bende ea n bmt md D a a e me iil EII x EIE X mi M i M EIA m IE pir HETEN SH it 0 it fl ll G1 I EHA Nl IT gt 22 gt 2222 2222 2222 Live K Freeze Auto The grid option is only available with the Live mode and Exposure mode You can remove the grid option by deselecting this option Stamp date and time You can activate or un activate the display of the status bar and of the date and time bar To proceed press the Insert date and time key CESIS E e SOE Nl IE 06 July 2009 13 27 ap gt gt gt 2 Tae 2 2 2 Hae 2D
74. ser profile 7 Help AutoScale Minimum conp man Gamma EEI Image analysis Controls Parameters The UVIsave 1D operating environment is organised into five areas 1 The menu bar The toolbar The functions folder The status bar The image window oR ON u ER AX wr a 2em bed Send to UVieolt pums Image analysis E Controls Parameters 53 128 Bt Paes fe ae Loe snc 1aKrAn Navigating the menu bar The main menu bar is organised into six areas File Edit Image analysis Text and enhancement Controls and parameters Window Help 1 2 3 4 op 6 T The following image illustrates the main menu bar File Controls amp parameters Window Help The File menu contains the user profile functions gt the file functions 5 gt the printing functions The following image illustrates the File menu The Edit menu contains gt the area of interest function gt the copy to clipboard function The following image illustrates the Edit menu Edit t Area of interest fgg Copy 54 128 i i L Fo SEE D 32 uta erat The Image analysis menu contains gt the MW analysis module gt The distance calculation module RF gt The colony counting gt The automatic image transfer to the UVlband software MY analysis Lu Aas Distance calculation AF Colony counting Send to mage analysis software Text amp The Text a
75. sign To come back to the default design click on the default design button Default design 67 128 Bt Peele JNF D JAEJHWAREAH Enhance Text Click on the Add text and symbols icon A pop up window displays the following menu Add text and symbols b Text editor t Date Time Full image name Short image name Font Font size Font color Text direction Terminal wilt v White Horizontal New Delete Symbol OK o C Vertical Enter the text in the text editor window gt Select the font gt Select the font size gt Select the font colour gt Click on OK to validate You can insert symbol by clicking on the Symbol button You can also add the following items to the image Date Add the current date to the image This date defaults to the date set on the computer you are using Time Add the current time to the image This time defaults to the time set on the computer you are using Full image name Add the image title to the image The title defaults to the file name and location of the opened image Short image name Add the image title to the image The title defaults to the file name of the opened image Enhance Reset enhancement Click on the Cancel image change s icon to undo all previous image treatments The original image is then displayed without all the further modifications 68 128 j Sab E d wo oo a A2evtiaerAt ON IM
76. stem is write protected to prevents the installation of any none approved programs or files such as malware or virus Thanks to the write protected function all files that are not write protected are removed with a restart of the system In other words a simple reboot will remove any malware or virus that would have been able to infect the UVISAVE system The Add a device function is designed to unlock the system to allow the installation of additional drivers such as printer drivers With the Add a device function the write protection is disabled after the system is restarted At that time you can install the needed drivers Upon reboot the system will have the new drivers installed and will be write protected again Before to add a device it is compulsory to scan for virus the USB key drive which will contain the driver to be installed in the UVISAVE system lt Validate using the OK key Os or select Cancel By validating the system will reboot to allow device installation or removal Upon reboot select the Add a device option When the system is unlocked the following display will appear UNLOCK MODE Switch off to lock the system 44 128 ne eee ere at strana Disconnect the camera from its USB port Connect a mouse and a keyboard to the UVIsave The Add a device display the following window Scan for installed printers Current printer HP LaserJet 5000 Series PCL6 v Define as default prin
77. ter Printer properties Delete the current printer Add a printer Scan for installed printers will retrieve the list of installed printer drivers The Current printer option lists the installed printers The Define as default printer set the selected printer as the default printer This means images will be printed with this default printer 45 128 iL DPE TP eee a2 strsrAd How to add a printer new printer driver installation To add a printer you need to connect a USB keyboard and a USB mouse The Add a device will then display the following window Scan for installed printers Current printer HP LaserJet 5000 Series PCL6 Define as default printer Printer properties Delete the current printer Add a printer Install the USB Printer Driver on a USB key drive Plug in the USB printer switch on the printer and wait for the Windows Add a Printer Wizard The following window will be displayed Found Hew Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install software for CanoniP A000 fe If pour hardware came with an installation CD lt or Floppy disk insert it now What do you want the wizard to do Click Next to continue Select Install from a list or specific location Advanced Then click on Next The following window will be displayed 46 128 Found Mew Hardware Wizard Please choose your search and insta
78. th the new mouse cursor click on the function from which you want to access the user manual You can access the help file index through the File Help from the Menu bar Index Using Help About 122 128 PES Pa 2 Ame rR strata UVISAVE HD2 MAINTENANCE Camera room The maximum ambient air temperature in which the UVIsave system should be used is 25 C Clean this part only with a soft dry cloth Working part Clean the tray by using a non abrasive solvent or tepid water Dry it with a soft cloth Air admission grid Never obstruct the air admission grids Regularly free all of them from dust 123 128 oe a E r Ii npp D JAEJHWAREAH UVISAVE HD2 TECHNICAL INFORMATION Electrical specifications Power supply 5 Current A 1A Fuse FST A 3 15A z Voltage V 100V 115V or 230V 5 z Frequency Hz 50 60Hz m Power 90 watts Fuses s Type FST Time lag T z 5x20 3 15A Climatic conditions z Altitude lt 2000 meters Operating humidity 20 to 70 no condensation allowed Operating temperature The maximum ambient temperature should be 25 C 124 128 i Te oan ea oe tat stra wera UVISAVE HD2 SPARE PARTS Spare parts UVisave HD2 with Tubes Tubes Starter Fuse 5x20 Filter with support BXT F T 8 WL T 8 M ST 151 Qty 100V transilluminator FG7 P 100V 230V el a UVISAVE HD2 re a es ee yl UVISAVE HD2 4 3 1
79. the AC voltage outlet Never pull the camera cable itself Disconnect the cable only by grasping the plug 7 128 D IRE sr ATA All the equipment connected to this unit shall be certified according to standard IEC 950 or other IEC ISO Standards applicable to the equipment Do not pour liquids directly on or inside the instrument Switch off all the lights immediately after use Clean the transilluminator platen after use The system should be located away from water solvents or a corrosive material on a bench top that is dry and stable The system should be placed away from interfering electrical signals and magnetic fields A dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your laboratory 8 128 32tvrahrAt TRADEMARKS Windows Windows XP Windows Vista Windows 7 Excel Microsoft and Power Point are trademarks of Microsoft Corporation Intel Pentium Intel Core are trademarks of Intel Corporation FireWire Mac and Mac OS X are trademarks of Apple Inc Coomassie is a trademark of BASF Aktiengesellschaft Cy is a trademark of GE Healthcare group companies SYBR Safe SYBR Green SYBR Gold and SYPRO are trademarks of Invitrogen Corporation Other brands or product names are trademarks of their respective holders This instrument must be used only by specialized personnel that know the health risks associated with UV radiation and with the reag
80. u bar 1 IMO000T2 2 C VLIMAGESIM000001 Eat 105 128 Bf Paes 2 eeF D se urrawtan A pop up window displays the print setup menu Print Setup ame OSes s Ready EPSON Stylus C70 Series USBOO1 rientation Portrait ize Bac feuille a feuille O Landscape gt Select a printer gt Click on Properties to modify the default setting of the printer if necessary gt Select the paper size and source select the orientation Click on OK to validate your options Note After you have installed and setup your printer the procedure for setting up and configuring a printer is the same as in other Windows program Autofit Click on the Autofit to resize the image to fit the size of the monitor The full resolution of the acquired may be larger than the screen resolution The navigation requires the Windows scroll bar The Autofit allows you to view the whole image regardless of the window size Typically reducing the size of a window also cuts off part of the image The Fit to Window option solves this problem by resizing the image so that it is always the same size as the window The Autofit feature proportions the display of the image to the screen resolution Autofit no scroll bar No Autofit scroll bar 106 128 a L oPELPeSee tat saat Default display Default display The Default display windows allows to gt monitor the image dynam
81. uration B ioo t DISPLAY gt Enhance the image ENHANCE sp The image dynamic refers to the range of grey levels in 7 between the minimum and the maximum pixel intensities obtained in an image Intensity Min Max SS Image depth is expressed as gradation level In an image the density range between white and black is divided into a number of gradation levels For instance a 12 bit image has 4096 gradation levels The image dynamic refers to the number of grey levels in between the minimum levels obtained and the maximum level obtained on a specific image The image dynamic status informs you of the obtained dynamic on your image compared to the potential image depth IMAGE DYNAMIC 58 128 i PRS Pee JAE VARA Display Autoscale Click on the Autoscale to resize the image to fit the size of the monitor The full resolution of the acquired may be larger than the screen resolution The navigation requires the Windows scroll bar The Autoscale allows you to view the whole image regardless of the window size Typically reducing the size of a window also cuts off part of the image The Fit to Window option solves this problem by resizing the image so that it is always the same size as the window The Autoscale feature proportions the display of the image to the screen resolution Click on the Inverse the image to inverse the grey level of the image This makes a negative image Before
82. ure 1 Port 4 The USB port is particularly designed to hold your USB key drive for image saving 15 128 Bl Pee a i a JAE HARD Camera uvitec camerie UVISAVE rear side drawing Port 2 Port 3 Main switch Port 1 Fuses Figure 3 16 128 D JAFARA Port 1 Power supply and main switch Port 2 Camera cable USB port Port 3 Printer cable USB port Port 4 Available USB port figure 1 gt Connect the USB Camera cable from the camera to the rear side USB port n 2 of the UVISAVE HD2 box as shown in figure 3 gt Connect the Power supply cable from the rear side of the system to an earth electrical outlet as shown in figure 3 A It is mandatory to connect the system to an appropriate AC voltage outlet that is properly grounded and protected by a circuit breaker Connecting to ground constitutes an obligatory protection 17 128 d i Oe Poe oe ee i tat straw UVISAVE HD2 UVISAVE 1D SOFTWARE INSTALLATION Software installation Preliminary steps UVISAVE 1D runs with Microsoft Windows XP SP2 amp upper or Microsoft Vista or upper 32 bit only operating systems Windows or Vista must be installed on your computer before any other installation Windows is a registered trademark of Microsoft Note During the driver installation the Windows installation CD ROM might be required Please check you have it before starting the UVIsave 1D software install
83. w gt Click on the Default button UVIlsave 1D calculates then the ideal values to be selected according to the parameters defined 107 128 i P SP ee D s2t strata Returning to the image main menu To return to the main menu click on the image capture icon u a u 3 auu EEM E NE ee E 88 88 88 EE 88 88 88 EE EE 82 82 88 i i i om E _ inal Eai A new menu appears with the main menu task bar functions 108 128 F ra TTT a D JAE WAREAH gt IMAGE ANALYSIS UVISOFT SOFTWARE Sending the image to the UViband analysis software 1 Select the Image analysis folder to access the analysis functions Image analysis 2 From the Image analysis folder click on the Send to image analysis software Image analysis Molecular weight Distance calculation RF Colony counting UVISoft analysis software The image is automatically sent to UVlsoft software such as UVlband UVImap or UVlbandmap 109 128 ee oe ie L F igp D ARE Urata ON CONTROLS AND PARAMETERS Accessing the controls amp parameters folder Select the Controls amp parameters folder to access these functions Controls Parameters Software parameters Good laboratory practice GLP view Other treatments BH Crop images EH Merge images UVisave 1D parameters The UVisave 1D parameters gather the optimum display parameter as well as the gamma parameter A pop up window displays the following menu

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