Neurosys Recording Hardware and Software User`s Manual
Contents
1. c Windows System32 NS Library dlil c Program Files NeurosysLLC NeuroPhys NS_Library h If you are running under 64 bit windows use this line instead gt gt loadlibrary c Windows SysWow64 NS_Library dll c Program Files x86 NeurosysLLC NeuroPhys NS_Library h If you are running this for the first time you may be prompted to selected a compiler by typing mex setup The default Icc win32 compiler will work fine Now within a Matlab program the following functions are available Calllib NS_Library NS_StartRecording boxNumber This causes NeuroPhys to start recording spikes boxNumber is an integer referring to the one based operant chamber number If you have only one chamber this should always be one Calllib NS_Library NS_SendEvent boxNumber eventNumber 0 This sends a timestamped event to NeuroPhys When you later analyze the PLX file generated by NeuroPhys this event will be recorded with a timestamp in sample counts at the moment this call was made There will be a slight delay due to communication from Matlab to NeuroPhys but this is typically only a few microseconds boxNumber is the operant box number one based as described above eventNumber is a number between 0 and 511 The last argument 0 is not used Calllib NS_Library NS_StopBox boxNumber This causes NeuroPhys to stop recording spikes in the specified operant chamber Cal2. the voltages at which each spike waveform intersects time cursor 1 and time cursor 2 respectively This might seem confusing at first To get some intuition on what is going on note in the example above that the dots are mostly in the upper left quadrant of the screen That is because most of the waveforms intersect the first time cursor at a negative voltage corresponding to an X value to the left of center and the second cursor at a positive voltage corresponding to a Y value above the center There is another important property of the time cursors in the example above I have placed time cursor 1 and 2 where the waveforms reach their approximate minimum and maximum voltages respectively This causes the dots corresponding to neural spikes to be as far as possible from background noise Notice also how the dots segregate into multiple distinct clusters these correspond to distinct neurons having distinct waveform shapes You can click and drag the mouse to outline a polygon that encircles one of the clusters This will cause all the waveforms corresponding to that cluster to display in a distinct color You can place multiple polygons and each will show up as a different color with successive clusters receiving a designated letter a b c etc This designation is saved along with the spike timestamp and waveform during a recording session The result should look something like the window below You ll see that I have
3. this This refers to the vertical cursors used to calculate the voltages in the 2 D cluster plots This option makes all channels have the same cursors as the window you just clicked Exclude channel This disables recording for this channel similar to the effect of disabling spike recording However when a channel is excluded its waveforms are no longer visible not even in greyed out form Also excluding a channel will significantly reduce CPU load because the computer will no longer have to calculate and display waveforms for this channel For more precise control over recording settings you can also use the Channel Manager to view and change all settings at a glance a FormChannelManager _ a ae Enable Enable Reference spike es ee es ee ee eaa Oo e Sans2 o ol ton i tamaa a e em 2 8 omas e eem 2 8 8 ee Channel 6 SS ee aoe e e Lo L Channel 8 1k 210m rN keom e Chama 10 e ef en e temai CS e a ema e eien Channel 13 Channel 14 Channel 15 Channel 16 Start test signal for selected box Use advanced software referencing wav file Sinusoid 1000 PE Square wave White Noise 19 If you have more than one recording chamber configured you will see one tab for each chamber with the tabs being named lt Box1 gt lt Box2 gt etc Unlike the menus above the channel manager allows you to select a separate ref
4. Neurosys Recording Hardware and Software Version 2 00 002 User s Manual 2008 2014 Neurosys LLC Contents System CU ST SUS ssri a 3 SUD Cl OO CS gcc sara eccentric nace sents E 4 Hardware ASSEN VN UNC COINS 2 acre teresa nocecetactsnis rE A 10 Install Sotware isere E E E E A EA EA E 11 COn y EE O a E 12 FRE SIP EINE spike SOUNE ear E 21 SPC SOTO gee ne E E ee ee ee eee eee 23 GROUNDING and SHIELDING cictecesenctssunstuiencamenssconectsecenesssiavsobidounesansiconsewaeetonie canes uivesnutnelerseations 24 ELECTRODE FABRICANON seserian A 25 APPENDIX 1 SoftWare SUS sonrada wucndened oeunursuoncnaenteusucvacniasscoaneeanoaceseeetes 27 APPENDIX 2 Matlab integration ccccccssccccsseccceesececceececeeeseceseeecesceneceesenseceeseneceseeneceesegeeeeees 30 APPENDIX 3 Med PC inte gration c cccccccscecscsecescesececccescecsccecscecsecaceesacsececaceecersesesaceecetacencarseeees 32 System requirements To use your system you will need the following items ees a Computer running Windows XP 7 or 8 One free PCIe slot a standard PCI slot will not work it must be PCIe At least 4GB of RAM Speakers plugged into computer s audio port to listen to neural signals If you have purchased a rat system you will need a Med Associates drug delivery arm and extra counterweight part numbers PHM 115 SAI and FAB PHM 110C to hold the commutator As of spring 2014 Med Associates charges 140 for both items together Th
5. are interested in spike recordings you will want to selectively view amplitudes that rise above background noise The program can selectively display and save such waveforms First make sure that the Show Spikes tab is selected Then click Show thresholded PURPLE arrow above to toggle thresholded mode which only displays waveforms in which the voltage exceeds a set threshold The threshold appears as a horizontal yellow line across each plot which you can drag up and down with the mouse If the threshold line seems to be missing it is sometimes at the top or bottom edge of the window simply grab it with the mouse to pull it back in You can also set thresholds automatically by clicking and holding the Auto threshold button this will set threshold to be 2 standard deviations below the mean voltage You have to hold down this button for a second or two for this to take effect and once you let go the threshold will lock in place In thresholded mode you will see 800us of each spike waveform with 200us shown before the threshold is hit and 600us shown after These can be changed in the settings Once a recording session starts detected spikes will be saved to disk but waveforms in between detected spikes will not be saved by default You can also view field potentials which are the input signals downsampled to one kilohertz To see these click on the Field Potentials tab There is another button green arro
6. ber from 16 to 32 Navigating the interface After you start the self test the waveforms you see in each window will represent 50 milliseconds of data i e 1 20 of a second To take a closer look at waveforms for any channel you can double click on any panel which toggles it to full size and hides the other 15 channels You can also click pause screen blue arrow below to freeze the display There is a row of buttons just below the main tool bar that allows you to select Session status Show Spikes Show Sort Clusters Rasters ISP anterspike interval histograms and Field potentials These are fairly self explanatory except for Sort Clusters which will be described later in this manual under Sorting page Error Bookmark not defined Switch to paneled view best for widescreen monitors Pause waveforms O K Tal 2 0 sere cama y as art DAQ Stop anne manager Tabbed view File Tools About V Pause Screen Keep window on top Show Show behavior status Show MSN code Show Spikes Sort clusters Rasters Field potentials detected Show thresholded Spikes box 1 Spikes box 2 spikes Uncheck the a A a Sh eee A X 0 to 45ms Mute Audio AA ia Y 39 1 to 39 1pV box to listen to spikes on computer s speaker Thin horizontal yellow line is Double click any panel to toggle full threshold for initial spike screen detection 15 If you
7. e delivery arm is visible here http www med associates com products page self administration drug delivery arms for rat drug delivery arm assembly with 38 gimbal ring It is best to purchase a second counterweight to balance the commutator call Med Associates for the part number as it is not listed on their website Materials for fabricating electrodes connectors insect pins cutter wire Please see Electrode Fabrication page Error Bookmark not defined Shipped contents 1 16 channel preamplifier If you purchased a 32 channel system you will have two of these 2 Rat systems only Rat headstage with retention clips and protective spring 3 Mouse systems only Mouse headstage with 18 ultra thin wire no retention clips or spring ut Ee 4 DAQ board from National Instruments Your board may say M series instead of X series but they are functionally similar x Series KELERGRES 11 13 i MuMifunction DAOG Derre Lha L aL THLE 5 24 cable to connect preamplifier and DAQ board 6 Extension cable for preamplifier input 60 long This allows amplifier and computer to be some distance away from the recording chamber 8 Self test board and stereo cable The stereo cable is used to connect the self test signal delivered from back of preamplifier to self test board The self test board can plug into either the headstage via the small white Omnetics c
8. ecording Disable all channels for spike recording Here is an explanation of each option Enable spike recording By default detected spikes are saved for each channel If you toggle this off then detected spikes are not saved and waveforms will display as grey instead of in color You should disable spike recording for channels that do not appear to have any useful neural spikes Store all samples If selected it is off by default then all samples not just detected spikes are saved Your file will become HUGE Most of the time you do not want this Reference all channels to this wire Your preamplifier has hardware reference capability i e the reference signal is subtracted before reaching the preamplifier By default all signals are referenced to ground but you can select any channel to be a reference 1 e its voltage will be subtracted from all other channels in this box When you select this all channels will have the same reference If you want some channels to have one reference and some to have another you can do this too but you must use the channel manager to do so Reference all channels to ground Self explanatory This is the default state when program is first launched Make all thresholds like this This refers to the threshold used to detect whether a spike has occurred or not This option makes all channels in this box have the same threshold as the window you just clicked on 18 Make all cursors like
9. erence for each channel In the above example channels 1 7 are referenced to ground while channels 9 12 are referenced to channel 8 The differential subtraction 1s performed prior to amplification which allows for excellent common mode rejection Any channel used as a reference will be highlighted in green You can also exclude some channels from processing by checking the very last column Exclude These channels will not be displayed and no calculations will be performed on them reducing CPU load This should be done if some wires are not connected on a particular rat or if a wire has gone bad and carries only noise Excluding a channel will also prevent a user from accidentally recording from it or using it as a reference for some other channel Note that excluded channels still contribute to a system s overall sample throughput Hence if you want to sample at a higher sampling rate than the default you should reduce the number of active channels in the program options rather than simply excluding them Where is my data By default all recorded waveforms are saved to in the folder NeuroPhysData under My Documents Each session will generate two files 1 A text TXT file containing session log details currently the session log shows the session duration and any text sent by Med Associates programs running concurrently with the recording session 2 A Plexon format file PLX containing spike times
10. gistration Registration Status WOT REGISTERED invalid key Registration Data Registration name JhouLab License Key Activation Key Run FREE version Register Product Simulate license emor Click Register Product Then select Import license from XML file and choose the license file you were sent If you don t have one you can run a FREE version that is fully functional forever but allows only 4 active channels 11 Conduct system self test There are two ways to test the software 1 If you are evaluating the software alone without hardware the system will automatically generate simulated signals to display In this case just launch NeuroPhys and skip directly to page 13 2 To test the software with hardware use the self test board shown below Connect it to the headstage and preamplifier as shown by the arrows below The two white Omnetics connectors mate to each other Each one has writing on one side and not the other make sure writing faces same side or else pins won t fit Connect audio cable here andem to back of preamplifier pamenee ae Ignore this black plug at first But if the self test fails you can test the amplifier alone by plugging this directly into the preamplifier bypassing f the headstage of Note that the Omnetics connectors each have writing on only one side a
11. ill want to test that they are connected with a continuity tester Once all wires are good apply dental cement over the pins to lock them into place 26 APPENDIX 1 Software settings D if you click on this wrench like symbol you will get a dialog with four pages of settings which are detailed below Page 1 Data folders behavior settings This page has the following settings First Box ID This sets the ID number of the first recording chamber This is one by default You can change it if you have multiple systems and you want each physical box to have a unique ID number For example if you have two 32 channel systems the first system s recording chambers will have ID numbers 1 and 2 the default and you can set the second system to start numbering chambers at 3 Number of operant chambers This sets the number of physical boxes in your system By default this will be the same as the number of recording chambers 1 e 1 for a 16 channel system and 2 for a 32 channel system Behavior outputs per operant chamber This is not used in the electrophysiology system and is intended for controlling additional hardware such as levers lights pumps etc Timing resolution This sets the resolution with which behavioral timestamps are recorded In most cases these timestamps will be received from external software such as Matlab Labview Med PC or other software Ephys data folder Folder to which standard ephys data
12. ing an extra wire very close to the recording wires and using it as a reference electrode This will subtract away almost all EMG noise Of course it will also subtract away any local field potentials LFP so if you are interested in recording LFPs this is not recommended If you are recording LFP s a preferred grounding method is to leave the chamber walls floors floating and ground the cabinet that the recording chamber 1s placed into This can be done by placing metal foil or mesh on the walls of the enclosing cabinet 24 ELECTRODE FABRICATION Electrode interface boards must be soldered onto Omnetics connectors part number A79014 001 As of Feb 21 2014 these connectors cost 35 26 each when purchased in quantities of 10 or more For more info see the following pages http www omnetics com neuro nano strip http www omnetics com neuro neuro_type aspx type A79014 001 amp amp category nstrip Attaching the connector is not difficult as the connector simply straddles the board in the obvious way After an experiment is over the connector and electrode board can be recovered by soaking the implant in acetone overnight You should be able to recycle it about 4 5 times before it wears out If you already use your own electrode designs make sure it has one of the following Omnetics male connectors with 18 signal pins and either 2 or 6 guide posts 1 A79014 2 A79016 3 A79018 4 A79040 5 A79042 Omnetics also
13. llib NS_Library NS_StartRecordingWideband boxNumber This causes NeuroPhys to start recording wideband signals 1 e it will record the raw voltages present at the A D board inputs without filtering away signals that are not 30 spikes This will generate very large files but you will have all possible information recorded This is useful if you don t know exactly how you will extract spike information and wish to process the session off line at a later time 31 APPENDIX 3 Med PC integration Probably the best way to illustrate Med PC integration is with a sample MPC file After installing NeuroPhys this file will appear in My Documents NeuroPhysData along with a couple of sample data files RecordingTest MPC Simple test file to show communication between MedPC and NeuroPhys LefthLever 1 Left lever is output 1 If your chamber is wired differently you should change this S456 el O sendeventname BOX 1 ProgramStart sendeventname BOX 2 LeverPress gt S2 G2 start startRecording BOX sendtext BOX Program starting 13 10 sendevent BOX 1 0O gt S3 Soy R LeftLever sendevent BOX 2 0O gt S4 Sa 5 stopBox BOX gt StopAbortFlush Copy the above file from My Documents NeuroPhysData into C MED PC IV MPC Then compile it using TRANS and run it from Med PC Make sure tha
14. me settings that were in use when the program previously closed 2 Load default settings 3 Load settings from a particular file Filter settings All spike and LFP channels have optional software filters These are 1 Attenuate low frequencies This option turns on a high pass second order butterworth filter that attenuates frequencies below the specified cutoff frequency 2 Attenuate high frequencies This turns on a low pass second order Butterworth filter that attenuates frequencies above the specified cutoff 3 Attenuate low high frequencies LFP These options do the same thing but for local field potentials Subtract periodic noise If checked this setting activates an averaging algorithm that removes any noise with a periodicity of 60 Hz Inverted inputs If checked this setting will invert the inputs 1 e make negative voltages positive and vice versa This should only be used if you have a preamplifier that has a negative gain value Test waveform settings This determines the type of waveform used for self testing 28 Page 3 Advanced electrophysiology settings This section has not been written yet 29 APPENDIX 2 Matlab integration If you are controlling behavior from Matlab you will want to send behavioral event timestamps to NeuroPhys To do this use the following code either from the Matlab prompt or within a m file If running 32 bit windows first use the following gt gt loadlibrary
15. nd not the other Make sure the writing on both connectors faces the same side otherwise the pins won t fit IF YOU HAVE TWO PREAMPLIFIERS START WITH AMPLIFIER 1 12 Once the program launches click Start Display RED arrow below amp NeuroPhys em i ace A Ly QO J S T va Start DAQ Stop DAQ Channel Manager Baie EAE PA Show session status Show code Show Spil Sort clusters Rasters ISI Field potentials Box status Odds gt Boxi Wideband Subject name AAE E E AE E e 10 S E ee E a AS No session rur No session running If you are evaluating the software without hardware then the software will automatically generate simulated signals to display and you should immediately see waveforms on the screen If you are testing a physical hardware amplifier and DAQ board you have to tell the system to generate a test waveform by clicking Channel Manager PURPLE arrow above which will bring up a dialog with a grid o x al FormChannelManager Cam on CIm Fo eto 2 a foudrfwemn a 8 6 CNE ora e i eon alala CE ketv 7 aala iea E pm e eem 2 oO e keme a a 00 O E wav file D Srusi Square wave White Noise Lok Click here to send test waveforms OO When this dialog opens click the button Start test signal for selected boxes 13 U
16. onnector or to the preamplifier itself via the large black connector p 1AT P g a ha baha Ba Bethe be BaMa by By Bi be be a 9 24VDC power adapter 10 Electrode interface boards rat version shown mouse version is smaller and lacks clip sockets oT Hardware assembly instructions Note that the Neurosys hardware and software can be installed in either order The software will run with simulated inputs if it does not find the A D board 1 Turn off your computer Install the National Instruments digital acquisition board DAQ board into your computer s motherboard You must have one available PCIe slot A regular non express PCI slot will not work If you have any other boards in your computer from National Instruments I recommend removing them to simplify the installation However if you would prefer not to remove them the program will prompt you by asking which card you want to use 2 Install the drivers for the acquisition board These are available by going to www ni com and searching for nidaq run time engine As of March 7 2014 the latest version of the nidaq driver is 9 8 http www ni com download ni daqmx run time engine 9 8 4298 en If you have NIDAQ already installed make sure it is at least version 9 6 Older versions may not work 3 NIDAQ is huge It will take a while to download and install probably about an hour You can and should go ahead and finish the res
17. outlined two clusters When you complete a session click on the red Stop button under the Session Status tab This button is immediately next to the green button you clicked to start the session You can now view your data file in the SpikeSorter program you have purchased The icon for the SpikeSorter looks like this 22 Spike Sorter The spike sorter is launched by clicking the following symbol There is currently no manual for the SpikeSorter but many of its features are similar to that of Plexon s Offline Sorter which can also be used to analyze the files produced by NeuroPhys Just play around with it and hopefully it is fairly self explanatory 23 GROUNDING and SHIELDING When you implant electrodes into your rat you will generally implant a ground screw into the cortex or cerebellum This will somewhat shield the recordings from environmental noise but it is generally not enough Ideally you should shield the recording chamber from outside noise There are many ways to do this If you are using a metal recording chamber the simplest way is to simply ground the walls and floor of the chamber However this will have the downside of introducing electromyographic EMG noise into the recordings whenever the rat touches the walls floor If you are only recording spiking activity and not local field potentials this might be acceptable because the EMG noise can largely be removed by plac
18. se this option to listen to spikes on computer speaker kag e k T Pas i se A amp iis _ LA f f at 7 G n a ee EN E RD Mu fe Audio s m ni Re B pa he a SNO do Your main window should now be filled with colorful waveforms which update every 50 milliseconds It will look like this but more animated Make sure Show Spikes is selected File Tools About ig K S EL Start DAQ Stop DAQ Show behavior status Show MSN code Show Spikes Sort clusters Rasters ISI Field potentials hres vold ed Spikes box 1 Spikes box 2 S E E aa a a a aa eae ee 1 Tabbed view X v Pause Screen Keep window on top EX Oto 45ms Y 39 1yV to 39 1pV i als ft ia ux 39 14V to 39 1pV U Box 1 Channel 1 Gain 1k Reference Animal Ground Mouse position 11 06ms Y 38 78uV I Elapsed time 00 00 16 If you do not see the signals above make sure that the Show Spikes tab is selected and that the preamplifier is connected to the DAQ board and powered on The software can automatically detect if the amplifier is plugged in and 1f it is not a warning will appear on the bottom status window When warnings are present the status window background will turn red and the spike display window backgrounds will also acquire a reddish tint If you still don t see the above waveforms after double checking connections and power please email me at
19. sells very similar looking connectors with 4 guide posts you can NOT use them as they have the signal pins in a 9x2 arrangement that will not fit the headstage If you build your electrodes using the provided interface boards you will also need stainless steel insect pins for binding wires into the holes You can get these from Amazon com Indigo Instruments or Bioquip Make sure you get the STAINLESS pins DO NOT ORDER THE BLACK PINS as they black paint is NOT conductive The exact pin size depends on the particular boards I sent you but are usually 00 0 or 1 I should have sent you some sample pins to get started When you order more pins they come in a package that looks like this 25 Insect pins Sains sre Stainless steel 100 pieces No 1 You will need a cutter for snipping off the end of the insect pins I recommend the Xuron 9180 Kevlar cutter http www amazon com Xuron XMS 9180 Finish Kevlar Serrated dp BOOO68P3T Y ref sr_1 1 1e UTF8 amp qid 1391901011 amp sr 8 amp keywords kevlar cutter The cutter looks like this You should cut the pins to a length of about 3 4mm If you cut them too short they will fall through the electrode board If they are too long that is actually workable but the electrode will be unnecessarily large Last but not least you need electrode wire I use A M systems formvar insulated nichrome wire catalog 761000 After the wires are pinned to the board you w
20. spikes and LFPs will be saved Wideband data folder Folder to which wideband data will be saved These files will be much larger than the spike and LFP files 21 Page 2 Basic ephys settings ID of first electrophysiology box By default this is the same as the first overall box ID If you change the ID of the first overall box e g from 1 to 3 in the example above then this will change accordingly This should be changed only if you have some operant chambers that are NOT configured for electrophysiology Number of electrophysiology boxes By default this is simple the total system channels divided by 16 Le it will be 2 for a 32 channel system and for a 16 channel system You should change this only if you are not using all available boxes e g if you only want to use one box in a 32 channel system This will reduce the number of displayed graphs and also reduce the CPU load Active electrophysiology channels per chamber This sets the number of channels used per recording chamber By reducing the number you can achieve higher sampling rates as the total system sampling rate is limited to 1MHz Acquisition sample rate This is a read only field It reports the achieved sampling rate LFP acquisition rate This is a read only field that reports the sample rate for local field potentials LFP The next three radio buttons determine where the program will get its settings whenever it starts up The choices are 1 Load sa
21. t NeuroPhys is already running before you run it The command StartRecording will cause NeuroPhys to start recording spikes This program will wait for a single press on the left lever and then quite 5 seconds later Note that all communication with NeuroPhys occurs via functions called from PASCAL so all such functions begin and end with a tilde symbol The command sendevent sends an event with the specified ID number 1 or 2 in the above example The command sendeventname is optional and is used to send a user 32 friendly name to NeuroPhys which will then write it into the PLX file header When you later open your PLX file in the spike sorter or in Neuroexplorer these user friendly names will appear and will make your life a lot easier Just before the Med PC program ends call St opBox to stop the recording session If you forget to include this line NeuroPhys will keep recording and you will have to stop it manually the red button on the box status tab 33
22. t of these steps while NIDAQ is installing 4 Connect the preamplifier to the DAQ card with the 24 cable The wide end of this cable plugs into the preamplifier green arrow below and the narrow end plugs into the back of the DAQ board If you purchased a 32 channel system there will be two preamplifiers two cables and two connectors on the DAQ board One of the DAQ board connectors will be labeled connector 0 al0 15 and the other will be labeled connector 1 ai16 32 Make note of which amplifier is plugged into which connector as connectors 0 and will appear in the software as recording chambers and 2 respectively AC adapter connects here Connect audio cable for self test 24 cable connects here 5 Plug the 24V AC power adapter into the socket marked 24 VDC 6 Rat systems only install the commutator into your recording chamber The commutator is designed to be held using a Med Associates drug delivery arm It should simply snap into place 10 Install software Download the installer file from www neurosysllc com Software html and run it It should install two programs NeuroPhys which is the main acquisition program and SpikeSorter which is an offline analysis program Now launch the acquisition program by clicking this icon You can also launch from Start menu NeurosysLLC Launch NeuroPhys At first launch NeuroPhys will ask you to register the product aO Product re
23. tamps and waveforms You can change the folders by clicking Tools Options 20 Real time spike sorting This system has the ability to sort 1 e classify spikes in real time To demonstrate these capabilities you can start a self test session as described above Then select paneled mode using the menu in the upper right of the main screen When running the self test your screen will look something like this Paneled mode Sort cluster code Show Spikes Rasters Feld potentials Gear all channels Ousters box 1 Gusters box 2 Show thresholded Spikes box 1 Spikes box 2 Clear this channel Horizontal line Voltage threshold Time cursor 1 Time cursor 2 In the example above I have enlarged channel by double clicking on it so that it is the only spike channel visible If you look closely you ll notice three cursors on this window shown by yellow dashed lines 1 A horizontal line that sets the voltage threshold at which a spike is detected 2 A pair of vertical cursors that defines two time points Each of these cursors can be adjusted by dragging it with the mouse You ll also see a panel in the upper right showing a bunch of dots that form sort clusters The dots work as follows 1 Each dot corresponds to one detected spike 1 e one waveform that exceeds the specified voltage threshold 21 2 The X and Y coordinates of each dot are
24. tom neurosysllc com or call 843 882 7128 j ia uau A warning will appear down here if amplifier is not plugged into DAQ board or 1s not turned on If you have two amplifiers the test signal will only be sent to whichever box is currently selected 1 e which box s tab is displayed in the channel manager To test the second chamber stop the Test Signal by pressing the same button you used to start it then select the other chamber in the Channel Manager tab and then restart the Test Signal Channels are grouped by operant box 14 If you bought a 16 channel system the system will assume you have one operant chamber and will assign all 16 channels to it If you bought a 32 channel system the system will assume you have 2 operant chambers each with 16 channels of recording capacity Data from these chambers boxes can be viewed and recorded independently In this case you ll notice that there will be TWO tabs with spike windows One will be labeled Spikes Box1 and the other Spikes Box2 and there will be 16 sub windows in each tab Likewise the channel manager will also have one tab for each box with each tab displaying 16 of the 32 total channels If you have a 32 channel system and want to change the defaults e g assign all channels to a single box then click the Tools menu Options Basic ephys settings and change the setting Active electrophysiology channels per cham
25. w above that toggles between the default tabbed view and a full screen PANELED view The latter mode allows you to see the cluster ISI and spike tabs simultaneously PANELED mode is highly recommended and works best with a widescreen monitor Start a simple recording session Once the self test signals are visible on the screen you can record them to a file Click on the first tab labeled Show session status Under the tab Box status there should be a grid with one row for every recording chamber and a green box in each row labeled Start Ephys as shown below 16 e a Aone K OKS TY mm Stop DAQ Channel ee ae ere ee Click here to start recording from box 1 Remember to Start the Display first EE ee 00 11 20 Each channel is referenced to ground by default However if you have a dedicated reference wire you can right click on that channel and select reference all channels to this wire By default ONLY DETECTED SPIKES are saved to disk 17 Modifying what signals get recorded If you click the right mouse button on any spike window you will see this menu which is used to modify how data is acquired and saved e Enable spike recording Store all samples not just spikes Reference all channels to this wire Reference all channels to ground Make all thresholds like this Make all cursors like this Exclude channel Enable all channels for spike r
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