Biofuge
Contents
1. Motor does not start Motor or rotor is blocked 1 Switch the instrument off and on again using the mains switch 2 Open the lid 3 Remove transport protection from the rotor 40 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken E 4 Error in temperature measure 1 Switch the instrument off and on again ment 2 If the error persists call Service E 7 Actual temperature outside toler Switch the instrument off and on again Should the display ance lt 10 C or gt 50 C E 7 persist the temperature control circuit is defective Please call your nearest Service E 8 Overvoltage or over current at Mains voltage outside tolerance Brake resistance defective the U F converter Switch the instrument off and on again If the problem persists call Service E 10 Wrong check sum in the NV Switch the instrument off and on again If the problem RAM persists call Service E 11 Error in data transfer from NV Switch the instrument off and on again If the problem RAM persists call Service E 23 Deviation in the internal tempera Switch the instrument off and on again If the problem ture adjustment persists call Service 41 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken Lid appears in the display Motor stops Rotor comes to a stop without braking The lid2. properly loaded rotors 26 Improper loading can in the worst case lead to damage to rotor and centrifuge Unbalance not only causes a noisy run but rapidly damages the motor suspension improperly loaded rotors When you have loaded the tubes fasten the rotor lid by screwing the cap nut centrally on it Close the lid of the centrifuge by firmly pressing it down There must be a clicking sound and the lid must be locked so that it cannot be opened manually Selecting the speed The minimum speed of the rotor is 2 000 rpm the maximum speed 13 000 rpm The built in microproces sor prevents higher or lower speed settings Between these extremes you can select the speed in steps of 100 rpm using the following procedure 1 Press one of the set keys increase or M decrease in the speed control section of the control panel cf foldout leaf in the cover By pressing the key briefly you increase or decrease the speed in steps of 100 rpm vA This option is supposed to be used for small changes and fine tuning 2 If you keep the key pressed the display changes at first slowly and after a few seconds at an ac celerated pace 3 Release the key as soon as you are close to the desired value and fine tune if necessary by re peatedly pressing the selected key or its coun terpart if you have proceeded too far in one direc tion The first digit after the decimal point
3. the pertinent safety procedures for handling infec tious material Pull mains plug Unscrew the rotor chuck Grab the rotor with both hands and pull it perpendicularly off the drive shaft Remove the centrifuge tubes and adapters and disinfect them or dispose of them as necessary Treat the rotor and the rotor lid according to the instructions given for the disinfectant soaking in liq uid or spraying You must strictly observe the specified action times Turn the rotor head down and drain off the disinfec tant Thereafter thoroughly rinse rotor and lid with water Dispose of the disinfectant according to valid regu lations Aluminum rotors have to be treated with anticorro sive protective oil subsequently 35 Maintenance and care Disinfection with bleaching lye These agents contain highly aggres sive hypochlorites and must not be used with aluminum rotors The following precautionary measures are to be taken for extensive protection of the 7500 3327 and 7500 3328 rotors 1 36 Avoid high temperatures The bleaching solution and the rotor should not be warmer than ca 25 C Do not let the bleaching solution act longer than absolutely necessary After disinfection rinse the rotor thoroughly with distilled water and allow to dry Decontamination For general radioactive decontamination use a solu tion of equal parts of 70 ethanol 10 SDS and
4. 0 5 0 6 ml 24 turquoise 7600 3758 reduction sleeve 6 x 46 mm 0 25 0 4 ml 24 red 7600 3759 13 for your notes hul Before use Where to install the centrifuge The centrifuge may only be used indoors Its location must meet the following criteria e A safety zone of 30 cm around the centrifuge must be maintained Hazardous materials must not be kept within this zone during centrifugation e The substructure must be stable and resonance free A good support is provided by a plane labora tory bench or a large laboratory carriage with cast ers that may be locked e To ensure sufficient air circulation a minimum dis tance from the wall of 10cm at the back and of 15 cm on each side must be kept e The centrifuge must be protected from heat and direct sunshine e The location should be well ventilated Before use Mains connection Make sure that the mains supply you use for the centri fuge meets the specifications printed on the type plate Turn the mains switch off press 0 only then connect the centrifuge with the mains supply using the power cord supplied with the instrument Removing the transport protection Turn the instrument on The display panel shows for about 6 s the routine internal software check se quence Open the lid by pressing the open lid key and remove the transport protection for the rotor Check that the rotor moves freely by lightly turning it and make sure the rotor i
5. 70056208 tubular socket wrench order no 2036 0072 Accessories The Biofuge fresco is delivered complete with a fixed angle rotor with 24 holes for placing microliter tubes with a volume of 1 5 or 2 0 ml In addition you may order three sets of adapters con taining 24 reduction sleeves each With these adapters you can centrifuge all commercially available microliter tubes with a volume between 0 2 and 0 6 ml as well as 0 2 ml PCR reaction vessels A further option is a PCR Strip rotor Please consult our sales documentation for a complete collection of accessories including technical data order numbers and special low cost package offers Accessories 11 Accessories Rotor program 24x2ml PP maximum speed Nmax min minimum speed Nmin min 2 000 2 000 maximum RCF value at Nmax 16 060 12 846 11 524 6 1 minimum radius cm acceleration deceleration time s min temperature at nmax C relative to room temperature 25 C permissible temperature range 4 C to 40 C 4 C to 40 C autoclavable number of cycles 121 C 10 cycles 121 C 10 cycles The values relate to vessel places 4 and 5 in the PCR Rotor 12 Adapters for rotor order no 7500 3328 Accessories Adapter Dimensions Capacity Number per Color Order No x H Set reduction sleeve PCR 6 2 x 20 mm 0 2 ml 24 gray 7600 3750 reduction sleeve 8 x 43 5 mm
6. another 2 seconds the display may read e g 475 8 03 NV RAM 4158 version 03 Troubleshooting 43 Troubleshooting for your notes t Technical data Technical data Component parts and performance Part function Description Design Armored case stainless steel Frame torsion resistant hot galvanized steel plates Body and front panel impact resistant highly dampening plastic Lid stove enameled steel plate with integral high resistance foam insulation Keys and display panel Keys and display panel covered with easy care protective foil Control elements User friendly Easycontrol system Material stainless steel Dimensions diameter x height 180 mm x 54 mm Electromagnetic release with key A when switched on 45 Technical data Function parameter environmental conditions permissible temperature of the environment dimensions H x W x D weight without rotor 46 Value indoor use max elevation 2000 m above sea level max relative humidity 80 up to 31 C linearly decreasing down to 50 relative humidity at 40 C 10 C to 35 C during operation no condensation 10 C to 50 C for storage and shipping 13 000 min 2 000 min 16 060 1 65 kNm 9 C to 40 C lt 55 dB A lt 45 dB A with standstill refrigeration 305 mm x 290 mm x 450 mm 27 kg The Easycontrol user interface Quick starting and stopping Indication of operating
7. pins under the side panels simul taneously from both sides until the lid unlocks audi bly Remove the auxiliary tools and open the lid 4 In case the rotor still turns close lid immediately and wait until it has come to a complete stop A Never brake the rotor using your hands or tools 5 As soon as the rotor stands still remove your sam ples and close the lid 19 Operation Inserting the rotor Improper or improperly combined accessories may cause severe damage to the centrifuge Rotors which are allowed for use in a Biofuge fresco centrifuge are detailed in the chapter Accessories and only these rotors are to be used in this centrifuge To insert the rotor you will need the cap nut and the socket wrench delivered with the centrifuge see the chapter The Biofuge fresco Pieces delivered Possible damage to drive and rotor You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and 30 C 20 Proceed as follows 1 Open the lid and make sure that the rotor chamber and the rotor are clean Remove eventual dust for eign material or sample residues The thread and the O Ring on the motor shaft must be in perfect condition Turn the rotor so that the notch for engaging the drive shaft points downward Place the rotor on top of the drive shaft so that the notch of the rotor is located precis
8. safety work bench before decontaminating it Danger of irreparable motor damage Never tilt the rotor Always grab it in the middle and pull out perpendicu larly 1 Open the lid of the centrifuge 2 Screw the cap nut open by turning it counterclock wise using the socket wrench delivered with the in strument Remove the cap nut 3 Grab the rotor in the middle and pull gently upwards off the drive shaft Be careful not to jam it 22 Loading the rotor Maximum loading Overloading may cause the rotor to explode Exploding parts may severely damage the centrifuge The Biofuge fresco can reach high rotational speeds implying enormous centrifugal force The rotors are designed in a way warranting sufficient residual strength even at the highest permissible speed However this safety system presupposes that the maximum permissible load of the rotor is not ex ceeded If you wish to centrifuge samples that together with the adapters exceed the maximum permissible load you must either reduce the sample volume or calculate the permissible speed Nyem according to the following for mula maximum permissible load n n perm Max actual load Operation Filling the centrifuge tubes Please note that plastic sample vessels only S have a limited service life particularly when Check carefully whether your used at maximum rpm or temperature and JE sample vessels are permissible for must b
9. time and temperature are indicated instead After the run the speed control panel displays End If you press the amp or M keys repeatedly you increase the corresponding preset value stepwise If you press and hold down the chosen key the respective value increases continuously at first slowly and after a few seconds at an accelerated pace The Biofuge fresco Temperature regulation of the Biofuge fresco During a run the spinning rotor creates frictional heat This leads to a temperature increase of the rotor the tubes and finally of the samples The extent of warming depends on run time e temperature of the environment location of the centrifuge rotor speed The Biofuge fresco is equipped with a powerful com pression type refrigeration Possible settings are from 9 C to 40 C For short term operation requiring precise temperature control both the rotor and the rotor chamber must be preadjusted to the desired tem perature Quick run operation As long as the quick run key is pressed the rotor is accelerated with maximum power potentially up to the maximum speed The Biofuge fresco Pieces delivered The Biofuge fresco is delivered complete with 10 a special cap nut for fixing the rotor 10 mm tubular socket wrench for fastening the cap nut fixed angle rotor 24 x 1 5 2 ml 7500 3328 cable for mains connection this Manual cap nut order no
10. wa ter Follow this with ethanol rinses then de ionized water rinses and dry with a soft absorbent cloth Ee Dispose of all washing solutions in appro priate radioactive waste containers Maintenance and care Autoclaving E Check whether autoclaving is permitted Never exceed the maximum permis sible values for autoclaving tem You may autoclave the rotor and the adapters at perature and autoclaving time 121 C Maximum permissible autoclaving cycle Should the rotor show hak of 20 min at 121 C wear you must stop using it S For safety reasons the 7500 3327 and 7500 3328 rotors must only be subjected to a maximum of 10 autoclavings The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on an even surface to avoid deformation Ee Chemical additives to the steam are not permitted 37 The Service of KENDRO Kendro Laboratory Products recommends annual servicing of the centrifuge and the accessories by the authorized service or skilled personnel The service provided by KENDRO comprises checking e the electrical installation e the suitability of the location e the lid lock mechanism and the safety circuit e the rotor e the rotor fastening and the drive shaft Defective parts are exchanged Besides the service personnel cleans the rotor chamber KENDRO offers inspection and serv
11. was manually opened during the run 1 Press the lid shut The instrument comes to a stop with out braking 2 If you want to continue the centrifugation you must switch the instrument off and on again The message br is displayed and the centrifuge brakes see br The safety circuit has been actuated 1 Pull the mains plug 2 Control ventilation slots and clean if necessary 3 After 20 min you can start the instrument again 4 If the safety circuit is again actuated call Service Display OPEN appears although lid is closed Start impossible The safety circuit has been actuated 1 Pull the mains plug 2 Control ventilation slots and clean if necessary 3 After 20 min you can start the instrument again 4 Ifthe safety circuit is again actuated call Service 42 In case you must call the Service Should you require our Service please tell us the order no and serial number of the instrument You find the pertinent information at the back of the instrument near the socket for the mains plug Moreover it is helpful for our service technician and saves you expenses to know the valid software ver sion You can determine the software version as fol lows 1 Switch the instrument off 2 Switch the instrument on The display reads 888 88 88 for a couple of sec onds Subsequently the display may read e g 0978 06 processor 0978 version 06 for about 2 seconds For approximately
12. 2 No 1010 2 020 94 For your safety Safety instructions in this manual A This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surround ings General hints are marked with this symbol In addition you are asked to adhere to the pertinent regulations in Germany Regulations for prevention of accidents BGV A2 Regulations for prevention of accidents VBG 5 Regulations for prevention of accidents VBG 7z Regulations for prevention of accidents BGV D4 For your safety for your notes The Biofuge fresco The figure below shows the Biofuge fresco with the lid opened In this state the standard display is speed x 1 000 and OPEN The Biofuge fresco Safety systems The Biofuge fresco is equipped with a number of safety systems Rotor chamber The rotor chamber consists of a stainless steel case which is sealed against the motor with a rubber cover When the lid is closed the rotor chamber is sealed against the surroundings by a rubber ring with a spe cial profile The rotor chamber is wrapped in evaporating tubing filed with the ecologically harmless cooling agent R134a which is free from fluorinated chlorinated hy drocarbons Warning if lid is manually opened during a run or if drive is overheated If the lid is manually opened during a run or if the tem perature of the drive exceeds a critical value a corre spon
13. 29 Stopping the centrifuge W u u dsseeeeerereennennee 30 Stopping with preset time 30 Stopping with continuous operation 30 Contents Short time centrifugation 1 u ssseeeeeeererrrnner 30 ROP Vale icid inanan rn dn studerer ale 31 Maintenance and care naasa 33 Maintenance to be performed by the customer 33 Cleaning eeen seer esss earn eeen ennen ke kassen nn an nn enes 33 Disinfection 1 seeeeeeeeseenesse sense keen seen ne s nke nnee 34 Decontamination W ssssseeeeeeeee nere nn rene 36 Autoclaving W ssseeeeeeerereeree nerne rente nen kr nen 37 The Service of KENDRO u ssseceeeeeeer kernen kernen 38 Warranty conditions u ssseseeereree reen nnnnnee 38 Troubleshooting sssssssssssnnnnnnnnsnnnns 39 Problems you can handle yourself 0008 39 In case you must call the Service 43 Technical data scenerne 45 Component parts and performance 45 The Easycontrol user interface 47 Electrical connections fUS S ccccccececceeeeees 49 NAEK coasts 51 Autoclaving Protocol scener 57 Speed RCF diagrams 1 58 For your safety Heraeus centrifuges are manufactured according to current technical standards and regulations Nonethe less centrifuges may pose dangers if e they are not used as designed e they are operated by untrained personnel e their design is improperly changed e the safety instructi
14. 31 rotating light 29 rotor cap nut for fixing 10 loading 26 partial loading 26 precooling inside centrifuge 28 removal 22 rotor cap 21 rotor chamber technical data 45 rotor insertion temperature 20 run time fine tuning 27 range 27 setting 27 running noise 39 RZB Wert 12 S safety instructions 3 4 safety measures 3 safety standards 5 safety systems built in 7 safety zone 3 30 cm around centrifuge 15 sample density maximum 3 sample temperature Index mimimum 8 service contracts 38 set keys 8 setting run time 27 speed 27 temperature 28 settings change during run 29 short time operation 30 site of installation 15 socket wrench 10 sodium fluorescein test solution for aerosol tightness 25 software check internal 17 software version determination 43 speed fine tuning 27 permissible 22 range 27 setting 27 speed of centrifugation for calculation of RCF value 31 start key 29 starting run 29 55 Index stopping 30 substructure 15 symbols for hazards and dangers 5 T technical data 45 temperature fine tuning 28 of sample 8 setting 28 temperature regulation 9 toxins protection against 4 transport precautions for 17 56 tube breakage with infectious material 34 tubes types 11 volume range 11 U unbalance 23 WwW warning lid open during run 7 warranty conditions 38 Autoclaving protocol Autoclaving protocol ope pee Tore Tee 57 Speed RCF diagrams
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16. Heraeus Biofuge prose Instructions for use How to use this manual Use this manual to get acquainted with your centrifuge and its accessories The manual helps you to avoid inappropriate handling Make sure to keep it always close to the centrifuge A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects This manual comprises chapters on Safety regulations Instrument description Rotor program and accessories Transportation and hook up Use of the centrifuge Maintenance and care Troubleshooting Technical data Index Overleaf you will find a graphic repre sentation of the control panel of the Biofuge fresco with a survey of the most important functions Please fold out speed run time temperature i quick run SRS 2c start BIOFUGE es Heraeus set keys mains switch The control panel of the Biofuge fresco Display Speed Resting Run End Stop run Time Resting end During run Temperature Resting end run Keys Start Quick run preset speed current speed rotating light rotor turns End error codes preset run time remaining run time or with quick start run time passed current temperature of sample normal start short term acceleration as long as key is pressed with indication of run time passed Open lid open lid possible only with mains switch ON Sto
17. care Maintenance to be performed by the customer For the protection of persons environment and mate rial you are obliged to clean the centrifuge regularly and to disinfect it if necessary Unsuitable cleaning agents or disin fection procedures may damage the centrifuge and its accessories If you intend to use cleaning agents or disinfection procedures not rec ommended by the manufacturer you have to make sure by consult ing the manufacturer that the procedure foreseen does not cause any damages to the instrument Maintenance and care Cleaning E Pull mains plug before cleaning the in strument Clean the casing the rotor chamber the rotor and the accessories regularly and in case of need This is indi cated both for reasons of hygiene and to prevent cor rosion due to contamination sticking to the instrument and its accessories Clean them with mild agents of pH values ranging from 6 to 8 For other cleaning agents please consult KENDRO Immediately after cleaning dry the aluminum parts or put them into a warm air dryer at a temperature not exceeding 50 C 33 Maintenance and care Disinfection During cleaning liquids and espe If a centrifuge tube containing infectious material leaks cially organic solvents should not during a run you have to disinfect the centrifuge im x come into contact with the drive mediately shaft and the ball bearing Organic solvents may decompo
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19. ding message appears in the display Lid and OPEN respectively Lid lock You can open the lid only when the power is turned on and the rotor has practically come to a halt lt 80 rpm You can start the centrifuge only if the lid is properly closed The Biofuge fresco Emergency lid release In order to permit you to remove samples even after a power failure the centrifuge is equipped with an emer gency lid release Features The Biofuge fresco is a refrigerated benchtop centri fuge for the preparation of sensitive samples in the biochemical and medical laboratory The powerful refrigeration permits at a room tempera ture of 25 C to maintain a sample temperature of 0 C over prolonged periods of time even at the maximum speed of 13 000 rpm The preset speed is reached in seconds You can also spin samples for only a few seconds using the quick run key I if this is required for the task in question The extremely long lived maintenance free induction motor provides quiet and vibration free operation even at high speeds The user friendly Easycontrol control panel permits easy operation With the centrifuge turned on and the lid closed the preset speed and run time and the ac tual temperature are displayed before the run During operation the control panel shows the actual values upon briefly pressing any one of the set keys amp or M the preset values for speed run
20. e of your samples please consider that the time required for temperature adjustment is prolonged The farther apart initial and final temperature the longer it takes for the tempera ture to adjust The temperature reading does not give the change in the temperature of the sample the reading is delayed with respect to the actual temperature change You cannot follow the heating or the cooling of the sam ples directly For critical applications you should take other precautions to ensure that the desired temperature is actually reached and maintained e g by measuring the tem perature immediately after the run Operation Starting the centrifuge Once the rotor is in place the main switch turned on and the lid closed you can start the centrifuge Press the start key in the control panel The centri fuge accelerates to the preselected value Simultane ously the run time display starts going backwards from the preset time giving the remaining run time in min utes After reaching the last minute the display switches to seconds remaining The rotating light tells you that the centrifuge is running During the run you cannot open the lid Changing the settings during the run You can change the settings while the rotor is spinning not in the quick run mode see section Short time centrifugation below The altered value flashes for a few seconds then changes to continuous display At the same time
21. e replaced as necessary the respective g value and reduce the speed if necessary The smaller the unbalance of the centrifuge the better the separation since separated zones are no longer perturbed by vibration It is therefore important to bal ance the centrifuge tubes as well as possible To minimize unbalance you should fill the tubes as evenly as possible You can achieve this by eye How ever you must nonetheless ensure that opposite tubes are filled to the same level 23 Operation Aerosol tight application not with open container lids The following steps have to be carried out e Lubricate the seals before inserting them lubricant order no 75003500 e Insert the seal C profile in the groove at the side of the body of the rotor e Insert the O ring into the inner groove on the screw on top 24 Attention Please check that your sample containers are suitable for the centrifugal application desired 16060 x g temperature in uncooled devices approx 10 K above room temperature Please observe the permissible filling volumes Nominal volume Permissible volume 2 0 ml 1 5 ml 1 5 ml 1 0 ml others nominal volume The sealing elements are to be checked regularly for damage to the shape and surface Exchange faulty parts immediately Spare sealing rings 75003268 Checking for aerosol tightness A Check the aerosol tightness of your rotor whenever ap
22. ease or lv decrease in the run temperature section of the control panel cf foldout leaf in the cover By pressing the key briefly you p p Ji increase or decrease the tempera ture in steps of 1 C This option is va supposed to be used for small changes and fine tuning 2 If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace 28 3 Release the key as soon as you are close to the desired value and fine tune if necessary by repeat edly pressing the key or its counterpart if you have proceeded too far The display flashes for a few seconds and then turns permanent The tempera ture setting is now stored The refrigeration starts operating at once if the prese lected temperature is below the temperature of the rotor chamber Bringing the rotor to the desired temperature in the centrifuge You can precool or preheat the rotor inside the centri fuge by using the following procedure 1 Insert the rotor if not already in place Attention To avoid jamming do not tilt the rotor Read the pertinent hints in the chapter Before use 2 Adjust the temperature as desired using the keys VA in the temperature control panel as described above 3 Set the speed to the maximum value 4 Select a run time of 15 min and start the centri fuge by briefly pressing the start key 2 If you wish to change the temperatur
23. ely above the re taining pin Push the rotor gently down until the thread is com pletely laid bare see figure If you have placed the rotor correctly you can screw on the cap nut easily and secure it with the tubular socket wrench delivered with the instrument Place the rotor cap onto the rotor Do not push the rotor down using force If you cannot screw on the cap nut you must carefully lift off the rotor and insert it again Ee Regularly check the proper positioning of the rotor and retighten the cap nut as needed Operation Permissible rotor temperature The rotors are only to be used within the temperature range from 4 C to 40 C Pre cooling in the freezer is not permitted Lifetime of the rotor There is no limitation on the service life of the high performance rotors However please observe the fol lowing due to safety reasons Rotors and accessories made of plastic should not be exposed to direct sunlight and UV rays If the rotor shows signs of discolor ation deformation or wear or is out of balance it must be exchanged straight away 21 Operation Removing the rotor To remove the rotor you must follow the steps de scribed above in reverse order With the hermetic lid you may in case of contamination separate the rotor from the drive without opening the lid In this case you can open rotor upon removal from the centrifuge using e g a
24. flashes for a few seconds and then turns permanent The speed is now stored Operation Selecting the run time You can select a run time between 1 and 99 min or continuous operation Preselected run time To predetermine the run time proceed as follows 1 Press one of the set keys amp increase or M de crease in the run time section of the control panel cf foldout leaf in the cover 1 5 T By pressing the key briefly you increase or decrease the preset run time in steps of 0 1 min va This option is supposed to be used for small changes and fine tuning 2 If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace 3 Release the key as soon as you are close to the desired value and fine tune if necessary by repeat edly pressing the key or its counterpart if you have proceeded too far The display flashes for a few seconds and then turns permanent The run time is now stored 27 Operation Continuous operation For continuous operation press the key J repeatedly or press and hold until hd for hold appears in the display With this setting the centrifuge keeps running until stopped manually er Please note that the lifetime of plastic tubes in particular is limited Extended use may damage them Setting the temperature The temperature is set as follows 1 Press one of the set keys E incr
25. for 5 hints symbol for 5 icons for denoting dangers and potential damage 5 indoor use 15 infectious material precautions in case of tube breakage 34 installation place of 15 Instructions for use delivered with instrument 10 items delivered 10 K key open lid 30 quick run 8 set 8 start 29 keys general operation 8 L lid blockage 40 lid lock built in safety system 7 Index lid open during run warning 7 lid opening 18 lid release emergency 8 18 manual 18 light rotating 29 location 15 mains connection 15 mains switch 17 maintenance 33 manual lid release 18 steps for 18 maximum permissible load formula for 22 maximum sample density 3 min temperature 12 minimum sample temperature 8 53 Index O open lid key 30 opening the lid 18 operation continuous 28 30 preselected run time 27 short time 30 organic solvents not allowed for cleaning 34 original parts mandatory use 4 overloading dangers implied 22 P partial loading of rotor 26 pathogenic microorganisms protection against 4 permissible speed 22 power on 17 power supply 15 precooling of rotor inside centrifuge 28 54 problems handling of 39 protective vessels for corrosive substances 4 Q quick run function 30 quick run key 8 R radius of centrifugation for calculation of RCF value 31 RCF value 31 readings of control panel during run 8 refrigeration properties 8 relative centrifugal force
26. for PCR Rotor 7500 3327 100000 13000 10000 speed rpm 1000 Imin 4 7 cm Tmax 6 8 cm 10 100 1000 1000 100000 RCF x g 12846 58 Speed RCF diagrams for 24 x 2ml Rotor 7500 3328 100000 13000 10000 speed rpm 1000 10 100 1000 RCF x g I 10000 W 16060 Imin 5 9 Cm max 8 5 cm 100000 59 Australia Austria Canada China Denmark France Germany India Italy Japan New Zealand Poland Portugal Spain Sweden Switzerland U K Ireland USA Kendro Kendro Kendro Kendro Kendro Kendro Labora Labora Labora Labora Labora Labora Axeb AB Albei Kendro Kendro Kendro AHSI S Labora Labora Labora Nippon Kendro Kendro Labora Kendro Sp lka Heraeus S A Heraeus S A Axeb AB Sollentuna Tel Kendro Kendro Kendro Kendro All other countries in Asia Pacific Kendro Europe Middle East Africa Latin America Internet Kendro Kendro Labora Labora Labora Labora Labora Labora Labora ory Produc ory Produci ory Produci ory Produc ory Produci ory Produc rtslund Te ory Produci ory Produc Co Ltd T ory Produc Massam Madrid Te ory Product ory Produc ory Produci ory Produci ory Produci ory Produc ory Produ
27. ice contracts cov ering these benefits Inspection costs are charged as flat rate contracts Necessary repairs are carried out free of cost during the warranty period and against payment after expira tion of the warranty 38 Warranty conditions The warranty period starts with the day of delivery Within the warranty period the centrifuge is repaired or replaced free of cost if there are demonstrable faults in materials or workmanship Conditions for a warranty are that e the centrifuge is used according to the instructions of use e installation additions adjustments changes or repairs are carried out exclusively by personnel au thorized for this by KENDRO e the required maintenance and care procedures are carried out regularly Troubleshooting Problems you can handle yourself Troubleshooting eS If problems other than those described in the following tables arise you must consult your nearest authorized service Error Behavior of the centrifuge Possible cause s and measures to be taken Displays remain dark The motor stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Is the mains switch turned on 2 Check the mains connection 3 If the mains connection is OK call the nearest Service Displays fail briefly The motor stops suddenly The rotor stops without braking The display reads br see br Brief interruption of main
28. ons are not heeded Therefore anybody concerned with operation and maintenance of the centrifuge must read and fol low the safety instructions In addition the pertinent regulations for prevention of accidents must be strictly followed EE This manual is an integral part of the centri fuge assembly and must be kept close at hand at all times For your safety Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively The maximum sample den sity is 1 2 g cm at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be stationed The centrifuge may cause harm to you or other per sons and may damage material goods if you do not respect the following safety measures Centrifuging hazardous materials e The centrifuge is neither made inert nor is it explo sion proof Therefore never use the centrifuge in an explosion prone environment e Explosive or flammable substances must not be centrifuged The same holds for substances prone to react briskly with each other For your safety Do not centrifuge toxic or radioactive substances or pathogenic microorganisms unless you have taken proper precautions Such precautions can e g consist of biological seals Should toxins or pathogenic substances enter the centrifuge or its parts you m
29. otor order no 7500 3328 13 Before Use ace case cdatecrcescceesessccesiccsnastazes 15 Where to install the centrifuge eee 15 Mains connection 2 ccccceeeeeeeeseceeeeeeeeeees 15 Removing the transport protection 0 15 Contents Operation 1 ere 17 Transport and installation 17 Mains connection W ssseeeeeeee ere te rren rn rrener 17 Opening the lid 11 W W W u ssssseeeeeeereeneeren kernen ternene 18 Emergency lid release 18 Inserting the rotor u ssseeeeeeeerereer renerne 20 Permissible rotor temperature 1 1 1 1 1 21 Lifetime of the rotor W u u u ssseeeeeeeererrer renee 21 Removing the rotor W W ssssceeeeereerre reen nere en eet 22 Loading the rotor eeecceeeeeseeeeeeeteeeeeenteeeeteees 22 Maximum loading cceececceeeeeeeeeeesteeeeeeees 22 Filling the centrifuge tubes 0 0 0 eee 23 Aerosol tight application zeer rs 24 Checking for aerosol tightne SS 25 Placing the tubes in the rotor 1 26 Selecting the speed ASSER SPA S 27 Selecting the run time M NSTRE 207 27 Preselected run time ES aE SER 177 27 Continuous operation 2 seeren 28 Setting the temperature ece 28 Bringing the rotor to the desired temperature in the center AE 28 Starting the centrifuge W u usssssceeeeeeerereen nerne 29 Changing the settings during the run
30. ou have to maintain a specific speed for your application During acceleration the time is counted forward in sec onds After 60 seconds the display changes to the minute mode RCF value The relative centrifugal force RCF is usually given in multiples of the earth gravity g It is a dimensionless number that allows one to compare the efficiency of separation or sedimentation of diverse instruments since it is independent of the instrument used The only values entered in the equation are radius and speed of centrifugation 2 RCF 1118 ke tr 1000 r radius of centrifugation in cm n speed in rpm At a speed of 13000 min the centrifuge achieves a maximum performance of 16060 g Check carefully whether your tubes are designed for this centrifugal force and reduce the speed if necessary Operation The figure for the maximum RCF value is based on the maximum radius of the tube Please note that this value becomes lower depending on the tubes and adapters used You may take this into account when calculating the RCF value for your application The figure on the last page of this manual gives a graphic representation of the relation between speed and RCF Apart from the maximum RCF value RCFmax lower line this graph also shows the minimum RCF value RCF pin calculated for the meniscus of the sample up per line 31 Operation for your notes N o Maintenance and
31. owing figure gives an example of possible read ings A detailed description of possible settings is given below va iva fra IE we Wi In this example the preset speed is 5 000 rpm the preset run time is 10 min and the current temperature reading is 21 C BIOFUGE t Heraeus Opening the lid For normal electrical unlocking connect the centrifuge to the mains supply turn the mains switch on and push the open lid key IA 18 Emergency lid release In case of a power failure you cannot open the lid nor mally using the open lid key see previous section To permit unloading even in this case the centrifuge is equipped with a mechanical lid unlocking system How ever you may use this system only in case of emer gency Rotor can spin at high speed A Touching it may cause severe injuries Always wait for several minutes until the rotor has come to a complete stop Without power the brake does not function and braking takes much longer than normal Should it be necessary to open the lid manually carry out the following steps Operation 1 Unplug the mains plug ting the mechanical emergency lid A Pull the mains switch before actua release 2 Make sure the rotor stands still 3 Push a thin screwdriver or another suitable tool horizontally from each side through the two open ings in the side panels of the centrifuge see figure Push the locking
32. p manual stop Set keys stepwise increase decrease of preset values accelerated change when pressed permanently Short pressing of any of the set keys switch from current to preset value Error codes troubleshooting see chapter Troubleshooting E 00 motor blockage transport protection removed E 4 error in temperature measurement E 7 actual temperature out of range E 8 excess voltage E 10 internal error call Service E 11 internal error call Service E 23 deviation in internal temperature calibration br power turned off during run or power failure Lid lid turned loose or opened during run OPEN with lid closed safety circuit triggered Warnings can span several display panels Contents For your safe EY 222 renen 3 Proper us SER aar alene er 3 Im proper USE annie a les ane 3 Centrifuging hazardous materials 1 1 1 1 1 1 1 3 m 2 1 ae sieec2te ites ceteeaet eed SEES SES RER EDER SERENE SES SELER SEES 4 Conformity to current standards 1 1 1 5 Safety instructions in this manual 1 1 1 1 1 1 5 The Biofuge fresco 7 Safety SYStEMS inonda 7 Feature Siriene diinan hania ea Saa 8 Temperature regulation of the Biofuge fresco 9 Quick run operation cccseceeeesteeeeeesteeeeeeees 9 Pieces delivered cccccceceeeeeeeeeeceeeeeeeeees 10 Accessories M G 11 Rotor program aa ora n cles 12 Adapters for r
33. propriate To carry out the test proceed as follows Carefully clean and degrease the rotor chamber wall then attach an adhesive white paper strip about 4 x 2 cm so that liquid leaking out of the ro tor may precipitate on it Fill all places of the respective rotor with water according to the following Table Insert the rotor into the centrifuge and fasten it Carefully place the amount of test liquid 0 5 sodium fluorescein in water specified in the col umn leakage test into the lower part of the rotor within a virtual circle comprising the vessel bores not the bores themselves using a pipette or sy ringe Place the rotor lid on top and screw it on ATTENTION Make sure that there is no spilled test liquid on the rotor clean if necessary Carry out a test run for 10 minutes at maximum rotor speed and 23 C ambient temperature Operation Check the paper strip under UV light preferentially in a darkened room If there is no detectable fluorescence the test is considered passed Finally rinse rotor rotor lid and lid seal in running water and allow to dry 25 Operation Placing the tubes in the rotor The rotor must be loaded symmetrically When loading the rotor only partially you must ensure that opposite bores always receive tubes of equal weight when cen trifuging a single sample place a centrifuge tube e g filled with water The following figure gives examples for proper loading
34. rrent Power consumption Fuses inside instrument 7500 5521 230 V 50 60 Hz 1 7 A 270 W 2 x 4 A slow blow 5 x 20 mm 7500 5522 120 V 60 Hz 3 8 A 270 W 1 x 6 25 A slow blow 6 3 x 32 mm The fuse may be replaced only by authorized servicing personnel 49 Technical data for your notes j LO Index A acceleration 8 accessories cap nut 10 rotor 11 tubular socket wrench 10 aerosol tightness check 25 aluminum rotor 35 autoclaving 37 autoclaving cycle permissible maximum 37 C cap nut for fixing rotor 10 centrifuge starting 29 centrifuge tubes Index types 11 volume range 11 cleaning 33 conditions of warranty 38 contamination necessary measures 34 continuous operation 28 30 control panel readings 8 coolant environmentally safe 7 corrosive substances protective vessels for 4 D damage symbol for potential 5 dangerous chemicals 3 decontamination 34 disinfectant 35 disinfection 4 procedure 35 51 Index disinfection with bleaching lye 36 display during run 29 displays brief failure 39 not lighted 39 E EC Guidelines 5 electrical connections 49 emergency lid release 18 error code br 40 Lid 42 OPEN 42 52 F fine tuning run time 27 speed setting 27 temperature 28 fixed angle rotor 11 fluorescein test solution for aerosol tightness 25 formula maximum permissible load 22 fuses 49 H hazardous substances 3 hazards symbols used
35. s supply 1 Check whether the plug is plugged in properly 2 Wait for 75 seconds 3 Restart the centrifuge Loud running noise Centrifuge is exceptionally noisy 1 Stop the centrifuge by pressing the key IB in case of emergency pull mains plug Wait until the centrifuge stands still Check whether the rotor is properly loaded Check whether a broken vessel damage to the rotor or motor malfunction was responsible for the noise 5 If you cannot locate and solve the problem call Service RON 39 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken Lid cannot be opened Pressing the open lid key has no effect A Lid not correctly engaged or lid warped 1 Check whether the mains supply is OK and the instru ment is switched on displays lit 2 Press the lid down in the middle of the front section and actuate the open lid key once again 3 In case these measures have not the desired effect you may open the lid with the emergency lid release see page 18 B Heat monitoring relays in the lid unlocking magnets have been actuated Press the key again after waiting for about 1 min br Instrument was switched off dur ing run or brief mains failure If the instrument was switched off inadvertently switch on again Wait for about 75 seconds The centrifuge comes to a stop without braking E 00
36. s tightly screwed on 15 Before use for your notes Operation Transport and installation Transport the centrifuge only in the upright position using the special box provided with the instrument and secure it properly Place the centrifuge carefully Before using the centrifuge make sure that the transport protection has been removed ee The Biofuge fresco is now ready for use In order to allow the coolant to settle down in the compressor the instrument must be left idle at the new location for about 1 2 to 1 hr Operation Mains connection Make sure that your mains voltage and frequency match the specifications on the instrument Turn off the mains switch on the lower right of the instrument push down the 0 marking then connect the instrument to the mains supply Turn on the mains switch on the lower right see fig ure mains switch For a couple of seconds the following reading appears in the control panel OEM DE OVA Va va HE BIOFUGE cst Heraguis BIOFUGE f Heras This tells you that the instrument carries out an internal check of its software After a couple of seconds the display changes The values now shown are except for the temperature the ones last used The temperature reading gives the current temperature of the sample before the start normally the temperature of the rotor chamber 17 Operation The foll
37. se i ig Infectious material could enter the centri the lubricant of the motor bearing fuge if bills or tube breakad occur The drive shaft may block p a i Danger of infection may occur upon contact Take appropriate protective measures for personnel Mind the permissible filling volumes and loading limits for the tubes Instruments with refrigeration unit Ee If a strong ice sheet is present in the internal chamber be sure to remove all condensate after defrosting In case of contamination the operator has to make sure that no further persons are i jeopardized Please control and clean the venting slots Contaminated parts have to be decon regularly taminated immediately If required further protective measures Before cleaning the venting slots have to be initiated AN please disconnect the centrifuge from the mains supply Please pull mains plug 34 Rotor and rotor chamber must be treated with a neu tral universal disinfectant Best suited for this purpose are disinfectant sprays ensuring that all rotor and accessory surfaces are covered evenly e Please use 70 ethanol for disinfection and handling hints when applying A Please note the safety measures these substances For other disinfectants please consult KENDRO Services Maintenance and care You may disinfect the rotor and the accessories as described in the following section Be sure to follow
38. state End of centrifugation Digital parameter display Speed selection Run time selection Temperature selection Technical data Performance Start key I Stop key E Quick run key I short time run when pressed permanently stop when released Spinning rotor indicated by rotating lights LED in the speed display panel Speed display reads End e speed e runtime e temperature adjustable in steps of 100 min in the range 2000 min to 13 000 min adjustable in minutes between 1 min and 99 min hd mode continuous operation adjustable in steps of 1 K between 9 C and 40 C 47 Technical data Time display in quick run mode between 1 s and 60 s in seconds above 60 s in minutes e Parameter memory for speed for run time for temperature Diagnostics lid not properly closed display OPEN general faults in performance error codes Testing standards 230V instruments Manufactured and tested in accordance with EN 61 010 1 EN 61 010 2 020 EN 61326 EN 61000 3 2 A14 2000 6 EN 55011 B 120 100V instruments Manufactured and tested in accordance with IEC 61010 1 1990 amendment 1 1992 amendment 2 1995 IEC 61010 2 020 1993 amendment 1 1996 CAN CSA C22 2 No 1010 1 92 CAN CSA C22 2 No 1010 1 B97 amendment 2 48 Electrical connections fuses Technical data Order no Voltage Frequency max cu
39. the new values are activated 29 Operation Stopping the centrifuge Stopping with preset time Normally the run time has been preselected and all you have to do is wait until the centrifuge terminates the run automatically As soon as the speed is close to zero the display reads End By pressing the open lid key you can now open the lid and remove the samples You can also terminate the run at any time as de scribed under Stopping with continuous operation Stopping with continuous operation If you have chosen continuous operation you must stop the centrifuge manually by pressing the stop key E jn the control panel The centrifuge starts braking at once and stops within a few seconds The speed dis play changes to End and the electrical unlocking mechanism of the lid is available You can now open the lid by pressing the open lid key IA 30 Short time centrifugation For short term operation the Biofuge fresco is equipped with the quick run function Short term centrifugation is started by pressing the quick run key ZZ continuously it stops as soon as the key is released In this mode the centrifuge accelerates with full power up to the maximum speed of 13 000 rpm unless you release the quick run key The preset speed is ig nored The centrifuge accelerates to the maximum speed of 13000 min ae Check carefully whether y
40. ust carry out the proper procedures for disinfection see Maintenance and care Disinfection Strongly corrosive substances that may cause dam age to materials and impair the mechanical strength of the rotor may be centrifuged only inside protective vessels Handling Never use the centrifuge unless the rotor is properly mounted Never manually open the lid if the rotor still turns Use only original parts for the centrifuge The only exception are common glass or plastic centrifuge tubes if these are approved for the rotor speed and RCF values of your rotor respectively Never use the centrifuge with the lid open Never use the centrifuge if the paneling has been partially or totally removed Changes in mechanical or electrical components may be carried out only by persons authorized to this effect by KENDRO Laboratory Products You may use the centrifuge only with a properly loaded rotor You must not overload the rotor If the rotor or the lid shows visible traces of corro sion or wear you must stop using it Strictly follow the rules and regulations for cleaning and disinfection Conformity to current standards Heraeus centrifuges are manufactured and tested according to the following standards and regulations CE for all voltages e IEC 1010 1 EN 61010 1 e IEC 1010 2 EN 61010 2 020 Pollution degree 2 Overvoltage category II for 110 V only e CAN CSA C22 2 No 1010 1 92 e CAN CSA C22
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