UB 48 Number 48 Synergy® Peptide Synthesizer Jaws Seals and
Contents
1. clockwise until the lower jaw seal comes free of the instrument Figure 3 You may need to use the small bar that is supplied with the T 5699 Tool to break the seal free Figure 3 Turning the T 5699 Tool to remove the lower jaw seal 6 Insert the T 5699 Tool into the upper jaw seal Figure 4 Turn the T 5699 Tool until its prongs engage the grooves on the jaw seal assembly Maintaining and Changing Synergy Jaw Seals and Gaskets Page 3 of 6 Page 4 of 6 7 Turn the T 5699 Tool in the reverse direction used to remove the lower jaw seal Use the small bar to help break the seal if necessary Figure 4 Inserting the T 5699 Tool to remove the upper jaw seal To inspect and clean the jaw seals and gaskets 1 Once the jaw seal is removed from the instrument gently and carefully pry the gasket from the end to avoid damaging either part 2 Inspect the gaskets and seals for cracks discoloration deformation or other signs of wear When necessary discard worn parts and replace them with new parts to ensure leak free operation 3 Use only methanol to clean the parts and allow them to dry before reassembling the autosampler jaws Figure 5 View of gasket in place on jaw seal Maintaining and Changing Synergy Jaw Seals and Gaskets To reassemble autosampler jaws 1 Replace clean or new gasket in the lower and upper jaw seals Figure 5 Use the rounded protuberance at the end of the T 5699 Tool to seat t2. User Bulletin Number 48 Synergy Peptide Synthesizer Jaws Seals and Gaskets March 1995 updated October 2001 SUBJECT Maintaining and Changing Synergy Jaw Seals and Gaskets Jaw seals P N 004183 and gaskets P N 004498 on the Synergy Peptide Synthesizer must be in good condition for optimum instrument performance Worn seals or gaskets dirt or contamination can cause leaks If the Synergy controller detects a leak at the jaws while pressure testing the AAC synthesis stops Occasional inspection of the jaw seals and gasket helps avoid these problems Inspect these parts every month for signs of wear such as cracks discoloration or deformation Replace worn parts annually or when defects are detected If the parts are not due for replacement and show no indications of wear you may continue to use them on the instrument after they have been cleaned WARNING Personal Injury Hazard The autosampler jaws are operated by a pneumatic valve under high pressure Painful injury can occur if the jaws close on fingers or hands Before working on the autosampler assembly close the main supply valve on top of the external gas tank and turn off the instrument power Routine Cleaning of Jaw Seals and Gaskets 1 o e ON Close the main supply valve on top of the external gas tank Turn off the Synergy power switch Remove the amino acid column wheel from its position on Synergy Dip a cotton swab into methanol once and squeeze ou
3. he gasket into the seal 2 Use the T 5699 Tool to replace the upper and lower jaw seals Turn the tool in the reverse direction used to remove the seals 3 Turn on the Synergy power switch and open the main supply valve on top of the external gas tank 4 Verify that the tank regulator setting returns to its original position 5 Perform the Leak Self Test described in Section 4 of the Synergy User s Manual to check the entire pressure system for leaks Maintaining and Changing Synergy Jaw Seals and Gaskets Page 5 of 6 lt L lt lt aaa Copyright 2001 Applied Biosystems All rights reserved For Research Use Only Not for use in diagnostic procedures ABI PRISM and its design and Applied Biosystems are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries ABI is a trademark of Applera Corporation or its subsidiaries in the U S and certain other countries All other trademarks are the sole property of their respective owners Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Stock Number 343825 002
4. t excess methanol Run the cotton swab around both the top and bottom jaw seal and gasket If the cotton swab comes away heavily soiled the seals or gaskets may be worn and need replacement Follow the procedure presented in this User Bulletin page 2 to remove the upper and lower jaw seals If the jaw seals and gaskets need no further attention turn on the Synergy power switch and open the main supply valve on top of the external gas tank Verify that the tank regulator setting returns to its original position gt Applied KS Biosystems Removal of Jaw You can remove jaw seals and gaskets from Synergy with the T 5699 Tool Figure 1 illustrates how this tool inserts into the jaw seals on Synergy Seals and Gaskets Page 2 of 6 T 5699 Tool Jaw seal with black gasket in place Figure 1 T 5699 Tool inserted into a jaw seal To remove the upper and lower jaw seals from the autosampler jaws 1 2 3 4 Close the main supply valve on top of the external gas tank Turn off the Synergy power switch Remove the amino acid column wheel from its position on Synergy Insert the T 5699 Tool into the lower jaw seal Figure 2 You may need to turn the T 5699 Tool until its prongs engage the grooves on the jaw seal assembly as illustrated by the dotted lines in Figure 1 Maintaining and Changing Synergy Jaw Seals and Gaskets Figure 2 Inserting T 5699 Tool into lower jaw 5 Turn the T 5699 Tool counter
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