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Leica DC Cameras with IA Applications
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1. From the Windows Control Panel select the Leica Imaging application the one that has the Leica logo this shows the Server select dialog Select the required driver from the Image Server configure dialog gt Select Image Driver Select Preview and see that the DC Twain window appears Now check OFF the DC Twain selection and check ON the Peripheral server and install it Please use the DM microscope SDK that is automatically installed from the Server CD Make sure you select the correct radio button for the model of DM Microscope you will be using If you have a later version e g V4 13 please completely uninstall this Also you must manually remove the remaining configuration files Step 3 If all is OK so far you should now be able to install the IA software as normal n Refer to Read the Release notes on the CDs before you start the installation A Hints If you have messages on starting the camera suggesting that the camera is not connected when it obviously is try closing the application and rename the LastUsed configuration file from the folder WinNT Twain_32 LeicaDC If these files are corrupt or not compatible the cameras will not start DC300 resolution is high and can be used with Leica QWin However you cannot see the whole image on the screen at full resolution so the DC300F and DC350F are better choices to use with Leica QWin With Leica QWin when using high res cameras go to preferences and check on all the f
2. uninstall is performed Also rename as old the configuration files in the Twain32 directory these are not necessarily compatible with a different version of DC Twain Install the DC Camera software first carefully following the instructions provided on the CD Make sure that this is working correctly e g using Windows Imaging PaintShop or IM 50 before installing Leica Servers Leica DC Camera with lA applications 1 Installation advice Issue 2 Feb 2002 Note If the cameras are not working do NOT proceed You will only compound any problems You must have the DC Cameras working independently of the IA applications before you install the IA applications Check the camera installation as described on the DC camera CDand retry the installation Step 2 Install Server CD You will find the user manual for the server on the CD Read it before proceeding Note Only install one server component at a time and check it is working correctly before you install another component You should only have one tick box set for installation at a time Warning the uninstall button will remove ALL servers not just those with ticks new to them Use with extream care Install Leica DC Twain from the Server CD Do not install the old drivers for DC cameras using PCI3 interface boards these boards are not compatible Do not re install any other drivers from the Server CD at this time for example M eteor drivers M ake sure all are checked OFF
3. Leica DC Cameras with IA Applications Installation advice Issue 2 Leica DC Cameras with IA Applications Installation Advice Issue 2 Abstract All reasonable steps have been taken to ensure that this publication is correct and complete but should any user be in doubt about any detail clarification may be sought from Leica Microsystems Imaging Solutions Ltd or their accredited representative The information in this document is subject to change without notice and should not be construed as a commitment by Leica Microsystems Imaging Solutions Ltd Leica Microsystems Imaging Solutions Ltd accepts no responsibility for any errors that may appear in this document rc Leica Microsystems Imaging Solutions Ltd England 2002 No part of this publication may be reproduced transmitted transcribed stored in any retrieval system or translated into any human or computer language by any means or in any form without the prior written permission of Leica Microsystems Imaging Solutions Ltd Microsoft and MS DOS are registered trademarks and Windows is a trademark of the Microsoft Corporation Date Feb 2002 Issue 2 Printed in UK Introduction The following notes aim to give some guidance in the installation and use of the Leica IA applications IW MW CW and FW that have been updated to work with the new DC cameras The compatible DC cameras are those that use the new PCI4 Hotlink board and the DC500 camera that uses the Firewire boa
4. lags fit to window etc However this can slow down image refresh so you have to decide which is the best compromise for the way you are using the software With Leica QWin make the image memory suitably large e g 100M in the System Configuration dialog Leica DC Camera with IA applications 2 Installation advice Issue 2 Feb 2002 For use with IA apps switch off the auto gamma and auto contrast in the DC Twain Do a Reset of these values If you leave these auto functions on the intensity of the image can vary this is OK for image documentation but can be a problem when you want to use a fixed detection threshold For use with IA apps do not use Auto Exposure for repeated image grabbing This function is in the Image Server dialog so it can be used to quickly get an image however once you know the settings to use Auto Exposure is not recommended as it slows down the acquisition rate For best colour rendition set the gamma to a value of 1 and only make small changes to gamma You will find that small changes to gamma can have a large effect on the image quality Note with the DC300F and DC350F there is a non linearity in the exposure time at around 114 5ms due to the physical properties of the camera it is best to adjust the light level so that you are faster or slower than this To display live images with fastest refresh rate you should have an acquire time of less than 114 msec Be aware that the resolution of the images disp
5. layed in live mode for DC300 amp DC500 is lower than the final acquired image For example you may see pixelation and aliasing This is reasonable so that you have a fast image refresh When you use a DC500 Leica QFluoro with narrow land filters the exposure can appear good on the screen live image but when you grab an image it is darker than expected for red and blue filters not green At the moment there is no explanation for this However when you switch the camera to 16 bit mode you can use the Auto scaling function to get high brightness images and this works well In Leica MW when using interactive measurements switch off fit to window this speeds up the image refresh Limitations A Note There are some image resolutions that do not acquire good images and these issues will be resolved in a later release These are DC500 3900 x 3090 9 shot and DC300 with the expanded resolution image For the DC500 using 2600 x 2060 16 shot please switch graphic display to 24 bit colour DC cameras cannot be used with Autofocus functions of the Peripheral Server as used by QWin and MW Some of the Leica MW processing functions e g QGrain are limited to 2k x 2k images due to the speed of processing of large images The resolution of the image selection with DC500 does not display correctly with DC Twain version 4 1 1 In general for IA applications you will find that images of 1280 x 1100 or smaller give good performance and you can
6. rd These notes are for use by qualified Leica personnel only al Note Additional information is in the release notes which are located on each of the application CDs Please read these before you start to install These notes are in pdf format The user manuals for the Server CD are also included on the Server CD in paf format CD s required for DC Camera use The following CD versions are essential for compatibility with DC cameras Earlier versions of the applications will not work with the new camera drivers DC cameras cannot be used with autofocus DC Camera Version 4 1 1 This is an interim version for use with IA software only Please do not use later versions until further notice as there are known incompatibilities E eica Server V2 1 2 rc D ica QWin V2 5 1 Leica MW V3 2 Leica FW V1 2 1 Installation WARNING These are for Windows 2000 only with at least 256MB ram installed in the PC For use with A images greater than 2k x 2k 512M B ram should be installed The simplest and easiest installation will be from a newly installed version of Windows 2000 that fas no previous drivers or applications installed After each stage of the installation re boot the PC Step 1 Install DC Camera software If you already have DC Camera software installed check that the cameras are working Completely uninstall any previous version of the DC Camera software unless it is the version specified above It is essential that this
7. see the whole image on a monitor set to a suitably high resolution These IA applications are for Windows 2000 only with at least 256MB ram installed in the PC For use with images greater than 2k x 2k 512M B ram should be installed or 768 M B ram with Qfluoro Leica DC Camera with IA applications 3 Installation advice Issue 2 Feb 2002 Leica Microsystems Imaging Solutions Ltd Telephone 44 1223 411101 gt Clifton Road Fax 44 1223 412526 etca Cambridge CB13QH imaging support leica microsystems com MICROSYSTEMS United Kingdom www microscopy imaging com
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