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GENESYS 10S UV-Vis User Guide

1. 6 Chapter2 Setting Up the Instrument LLusuuuueeeeeeee enn BB 7 Entering Parameter Values p02 eben eee hewn eek Ree eke eee re Rb 7 INumetic Entty i estre Rep ede ERG b rareiaec olin RA na eae 7 Menu SElECUON sse taae 8 On Off Vogels qe T E TT 8 Alphanumeric Entry uei oat de bci EY abba ee ON acabe do eh 8 Setting Utility Parameters ca aequa s eee xoa a on OC CR e OU E A 9 Setting the Date and Times dosi cda ba e che cee Ey cabs 9 Standby M OUO out toate eee k MORI RR at tO patat bp e 11 Setting Baseline Expiration Time e oos pis aiecsasatecsdrayiebieeyaes 11 Setting the Screen COBIDIOS L ca uto ds aed ance Rug a e x 08 do ap des ata nates 11 Setting Up the Internal Printer Lasse eed o Dee E nee EE eR ERE 12 Setting the Utility Parameters for the Printer llle 12 Chapter3 Accessories ciiococosseu ru me o Ub EXsu ipe DOiRCade qua a paa Ead pad Ks 15 Cell Holders and Cell Holder Accessories 1 222 o scere ended 15 Cell Holder Configurations sdsscew see chew cR eer dans wae sees 15 Cell Holder Initialization eas xe bot ciens De Soeur ae Sedo 18 Changing Cell alder ap iin e Ct oe eek eee QE iep e a UR 18 Installing the 6 Position Cell Holder and the Single Cell Holder 19 Removing the 6 Position Cell Holder and the Single Cell Holder 20 Installing Accessory Cell Holders 22292 apretar dadewsatenee sans 20 Thermo Scient
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3. 3 Accessories Cell Holders and Cell Holder Accessories Cell Changer System Single Cell System Combination Systems Not applicable Must be installed in the B 2 and 4 positions Cell Holder Initialization When you press Run Test to start a measurement the instrument displays the message Calibrating and Checking Turret Please Wait while it attempts to initialize the cell changer at the Blank position If a single cell holder has been installed the message Error Single Cell Holder found Use Single Cell Holder appears Press Accept Change to continue or install the cell changer and press Cancel Change If the 6 Position Cell Holder has been installed it will initialize it to the B position After it has initialized the cell changer the instrument will display the data collection screen for the test Note If while in this screen you remove the cell changer and press Run Sample the instrument will display Fatal Error 8 Press Esc to return to main menu Pressing Esc returns you to the parameter menu screen of the test Press Run Test and the instrument will now display the message Calibrating and Checking Turret Please Wait while it attempts to initialize the cell positioner at the Blank position If the cell changer is still not in the sample compartment the instrument will display Error Single Cell Holder Found Use Single Cell Holder Either press Cancel Change and reins
4. Lock Unlock Used to protect stored tests from accidental deletion or alteration asks for a password to allow the user to lock or unlock the file Utility Low High Limits Enters the lowest and highest acceptable results outside of which the result is flagged as Low or High Adv A T C Std Curve Abs Ratio Abs Diff Kinetics 3 Pt Net and some Bio Tests Math Accesses manipulation functions of the graph Scanning Measure Blank Initiates measurement of the blank as function key Measure Blank as test parameter Selects the frequency of zeroing the instrument Once or Every Reading Kinetics GENESYS 10S UV Vis User Guide 121 23 Parameters Parameter Measurement Mode Description Selects the type of photometric data reported for a measurement Abs T Conc in A T C Kinetics Scanning Multiwavelength Measure Samples Initiates measurement of samples Max X Enters a maximum X value to manually rescale the graph Kinetics Scanning Max Y Enters a maximum Y value to manually rescale the graph Kinetics Scanning Min X Enters a minimum X value to manually rescale the graph Kinetics Scanning Min Y Enters a minimum Y value to manually rescale the graph Molarity of cation Kinetics Scanning Enters the molarity of Na in the incubation mixture T4 calculation in Oligos tests Next Cursor Selects a cursor point in functions using more than one cur
5. 3 Remove the power cord 4 Insert a flat blade screwdriver into the notch on the fuse cover and pry off the cover 114 GENESYS 10S UV Vis User Guide Thermo Scientific 22 Maintenance Changing the Fuse 5 Use a flat blade screwdriver to remove the fuse holder 6 Unsnap both fuses to remove them 7 Insert the new fuses pushing them in so they snap into place 8 Replace the fuse cover 9 Replace the power cord 10 Plug the instrument back in to the wall outlet or power strip and turn on the power Note If the fuse blows again contact your distributor or technical support Thermo Scientific GENESYS 10S UV Vis User Guide 115 22 Maintenance Changing the Fuse 116 GENESYS 10S UV Vis User Guide Thermo Scientific LL m Parameters Thermo Scientific Parameter x Description Enters math operators when in Calculator mode Utility Formamide Enters percentage of formamide contained in the sample Oligos tests GC Calculates percentage of GC pairs contained in the sample Oligos tests Mismatch Enters the Mismatch value to calculate Tm Oligos tests of lamp life used Displays the estimated percentage of lamp life used based on a typical lamp life of five years Utility 3 Pt Net Lets you calculate peak height from the tangential baseline in the graph Scanning Absorbance Enters the absorb
6. Calculation s 2 o J Xi N n 1 Where N Degree of polynomial ISSXY JSQX SOY The calculation for the correlation coefficient applies only to first order linear regression curves first degree polynomials oo SH fasi N 1 24 Calculations for Software Graphs Absorbance ratio Abs4 AbsA ADS ep Abs ir Absi Abs 2 or 2 ref Absorbance Difference Thermo Scientific Result AbsA factor 1 AbsA factor 2 or 4s Abs factor 1 4bsA AbsA factor 2 GENESYS 10S UV Vis User Guide 129 24 Calculations for Software Calculation Calculation s 3 Point Net Baseline corrected absorbance A 4 la A a Graphs CORRECTED ABSORBANCE he WAVELENGTH 3 Point Net Absorbance sample curve 3 Point Net Baseline corrected absorbance ASTM E169 04 L A A EE ls E A Kei 130 GENESYS 10S UV Vis User Guide Thermo Scientific M Em Calculations for Oligo Calculator Calculation of bases Entry Parameters Repetitive sequence of A T or U Gand C Formula Count total number of bases entered Displayed Units Length of bases GC content Molecular weight Use AT U GC sequence entered above units A units T units G units C units U of G C bases x 100 total of AT or U GC GC If entry d
7. Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position Press Measure Sample If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually GENESYS 10S UV Vis User Guide 73 16 Absorbance Ratio Measuring Samples 74 GENESYS 10S UV Vis User Guide Thermo Scientific 3m Absorbance Difference The Absorbance Difference application lets you measure the difference in absorption at two different wavelengths Reference wavelength correction is available to eliminate the effects of a sample matrix Typically used in quality control applications an absorbance difference application provides a convenient and quick diagnostic test for sample quality Use Absorbance Difference for e Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples To get started press Test highlight Absorbance Difference and press Enter fibsorbance Difference 12 5 St 3Jan02 Test Name Wavelength 1 546 Onm Wavelength 2 546 Onm Ref Wavelength Correction Off Factor 1 1 000 Factor 2 1 000 Units ppm Sample Positioner Auto 6 Number of Samples ore parameters Press f or to select item to change LER Due mu Test Tests Test Recalling a Test To
8. Read only Read only Read only Read only Read only Read only Read only Read only Read only 6 Managing Stored Tests Lock Unlock Note To view the stored tests of a particular test type press Test select a test type and press Stored Tests To lock or unlock a test highlight it and press Lock Unlock Enter the password and press Enter Utility Test Directory Protein Tests Louru Micro BCA Standard BCA Micro Biuret Direct UU 280 Direct UU 205 Warburg Christian Cell Growth Cell Growth 9 31 22Feb0 Read only Read only Read only Read only Read only Read only Read only Read only Scanning fissaub1 VATA TUE More tests Press f or to select test F WEE M Test Test Test Note To lock or unlock access to the file you must enter the software password of this manual Deleting a Test Thermo Scientific To delete a test highlight it and press Delete Test GENESYS 10S UV Vis User Guide 35 6 Managing Stored Tests Deleting a Test 36 GENESYS 10S UV Vis User Guide Thermo Scientific MEN uU SmartStart The SmartStart feature lets you customize the spectrophotometer by placing the most frequently used test methods on the first menu Right after the instrument initializes a simple menu containing only the SmartStart tests appears If you select one test as a SmartStart test the instrument when powered on automatically loads this test and prepares for i
9. Scientific 20 Kinetics Rescaling and Recalculating Tabular Kinetics Results The instrument redraws the plot using the selected minimum and maximum values and displays the recalculated rate and result Rescaling and Recalculating Tabular Kinetics Results When working with tabular kinetics results you must press Edit Data before you can rescale and recalculate After collecting kinetics data you can use all the data for the rate calculation or select specific start and end times When you modify the start and end times or select all the data the instrument recalculates and displays the new reaction rate and result The edit screen lets you Use all the data to recalculate the results Select specific start and end times for the rate calculation and recalculate the results Kinetics 12 57 30Jan01 Test Name KINETICS Cell 1 HH MM SS fibs Delta Lin 0 0 0 0 0 0 0 IDit min Abs Result 1 0 000 0 013 Press fT or for more data data Graph Time Time data To use all the data to calculate the reaction rate With your table of kinetics data displayed on the edit screen press Use All Data The instrument calculates and displays the rate To select specific start and end times for the rate calculation 1 With your kinetics data displayed on the edit screen highlight the appropriate data point in the table 2 Press Start Time or End Time to display the recalculated rate and result Thermo S
10. Thermo Scientific GENESYS 10S UV Vis User Guide 67 15 Multiwavelength Setting Up Test Parameters This screen provides these capabilities Setting Up Test Parameters Cell Correction Saving a Test Viewing the list of stored tests Taking Measurements Setting Up Test Parameters To set up test parameters In the Multiwavelength screen highlight the desired parameter See the procedures below for instructions on adding or deleting wavelengths and factors If you have previously selected the wavelengths to measure press Save Test to save the test or Run Test to measure a blank or samples If you have not selected the wavelengths you can add wavelengths and factors as shown below Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Adding Wavelengths and Factors Note You can enter factors only when the measurement mode is set to Concentration Factor To add wavelengths and factors 1 Press Set nms 68 GENESYS 10S UV Vis User Guide Thermo Scientific 15 Multiwavelength Taking Measurements Multiwavelength Test Name WLH nm Factor 1 340 0 56 85 2 532 0 630 8 3 633 0 0 052 age 1 ress T or to view data 2 Highlight a position for entering the first wavelength and factor
11. UV Vis User Guide 31 6 Managing Stored Tests Saving a Test Create Test Name 12 21 21Feb07 ABCDEFGH I JKLMNOPQRS TUUUX YZ0O 123456789 abcdefghijklmnopqrstuvuxuz _ pO Test Name Character Name This screen lets you Delete the test name Delete a character in the name Add a character to the name Accept the name 2 Highlight the first character for the test name and press Add Character 3 Continue selecting and adding characters until the name is complete 4 Press Accept Name Saving a Test After a method has been configured there are two options for saving a test The test method can be saved to an external memory device using the front USB port or to the internal memory of the spectrophotometer 32 GENESYS 10S UV Vis User Guide Thermo Scientific Thermo Scientific 6 Managing Stored Tests Saving a Test Scanning Test Name Start Wavelength Stop Wavelength Sample Positioner Scan Speed Interval Cell Correction IDH O OFF uto Print uto Save Data To save a test in the instrument library 1 Press Save Test A prompt appears Save as an internal test Save test to the USB drive Save test to internal and USB Press f or to select Press ENTER Press ESC to exit Cell Co ID CO OFF 1 uto Print On uto Save Data Off ress T or to select item to change 2 Select the appropriate location and press Enter 3 Create or enter a name for th
12. Units Delete nm Removes a wavelength and factor from the list Multiwavelength and some Performance Verification tests Diluent Volume Enters the volume of diluent added before measurement Dilution Multiplier in some Bio Tests Dilution Multiplier Displays the factor used to correct for sample dilution Display Activity Indicates whether results should include protein concentration DNA 260 Calculates the extinction coefficient DNA Factor Enter the factor to calculate DNA concentration DNA Bio Tests Edit Lets you change a wavelength or factor in the list Multiwavelength and some Performance Verification tests Edit Curve Lets you manipulate the graph Kinetics Edit Data Lets you select a portion of the data in a table for recalculation of a result Kinetics and Scanning Edit Graph Lets you manipulate the graph Scanning Edit Scale Lets you change the graph axis scales and view individual data points Scanning GENESYS 10S UV Vis User Guide 119 23 Parameters Parameter Factor Description Enters a factor to convert a datum to a result Abs x Factor 1 Concentration Result Abs min x Factor 2 Kinetics Result Can be entered or calculated from concentration and absorbance values in ADV A T C Factor 1 Enters a factor to convert a datum to a result Abs WL1 x Factor Result Abs Ratio Abs Diff Multiwavelength tests Factor 2 Enters a factor to convert a da
13. a test continues to fail after you have tried all these recommendations follow the troubleshooting list for the test being run included with the description of each test Make sure You follow the instructions for the test properly Filters and standards are clean The sample compartment door is closed during the test The sample compartment is clear of obstructions The cell holder assembly is installed properly If the 6 Position Cell Holder is installed run the test once with the sample compartment door open to verify that the 6 Position Cell Holder is moving smoothly No problems are indicated by the power on diagnostics after you turn the instrument power OFF and then ON The lamp is ON The lamp compartment is clear of obstructions WARNING Do not open the lamp compartment unless the instrument power is OFF WARNING Do not turn the instrument power ON unless the lamp compartment is closed 100 GENESYS 10S UV Vis User Guide Thermo Scientific 21 Performance Verification Wavelength Accuracy Internal Wavelength Accuracy Internal This test locates the peaks of the internal xenon lamp and displays the expected and measured wavelengths for the peaks A xenon lamp has strong fundamental lines at 229 nm 529 nm and 883 nm These lines are an essential property of xenon and serve as a fundamental standard When running the internal standard test remember that The wavelengths and tolerance values
14. access to Cell Correction Note Cell Correction is not active from the Main Basic ATC screen Note Cell Correction is active only when the 6 Position Cell Holder is set to either Auto 6 or Auto 3 The feature is not active when the cell holder is set to 1 Cell or Manual 6 nor when the Single Cell Holder is installed Before running Cell Correction Clean the inside and outside of all the cells to be matched Fill the cells with distilled water or other blank solution and place them in the sample compartment see Selecting and Positioning Cuvettes on page 5 Place the blank cuvette in Cell B of the cell changer Cell Correction Torun Cell Correction 1 Load the test type or stored test Advanced amp zT C ef Wavelength Correction ef Wavelength Delay Time min sec 0 00 Sample Positioner Auto 6 umber of Samples 1 More parameters ress T or to select item to change er exta fen Test Tests Test 2 If Cell Correction is not visible highlight More parameters and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 2 5 Cell Correction Cell Correction 3 Highlight Cell Correction and press Enter Advanced amp zT C ell Correction Correction ID CO OFF 1 ow High Limits 999979999 tatistics off More parameters Cell Correction Not Calculated ress T or to select item to chan e Cell Corr
15. ai Pare apo Pa S ADDED SHEER arta 54 Entering a Factor cs essc bv arre ne So che sek tre pie Wee aeeis 55 Measuring Sata plesoeus Pansat Exahedce decir o Soa du Sti sr as pur a 55 SCANNING sinon tO Ta pae e Ue ates dete sce ea dare fe mter dm qus 57 Recalling a T estos opos os oet laurie Pots E aea dae e Peleus et 57 Setting Up Test Patameters oo nesp i ae DIY p Pep AV E CE FRA oe eed 58 Collecting a Baseline Seas ois eis dne Yn eer anaes een e a PRA Re 58 Scanning a Sain ples ose er RANA E Mone eps bed ci dated a i 59 Viewing and Manipulating Scan Data basis beue Eri EXEE ewes 60 Rescaling Graphical Scan Data uia adatto Parthis ant tie Mgr yid 60 Performing Calculations on the Scan Data 000 eee eee 62 Labeling Peaks and Valleys 5 20 oes ed an ebbe Rari bari 62 Smoothing D3t3 Lio 4 Fus RE deci Era ops doe M E 63 Determining Peak Height Using a 3 point Net Equation 63 Calculating the Area Under a Curve o a ero rao Rb T e WX 64 Viewing and Rescaling Tabular Scan Data ao cce so eee ne ae 65 Multiwavelength usuueeeeeseseer IH In 67 Recallinsa Lesbos cus cs butt C a eter qat n eaten M aceto ues tete us 67 Setting Up Test Parameters so biet bares Me gabe MAD St A o ang 68 Adding Wavelengths and Factors e Lom eae Deom e eb Dnm a HOCH ERE v RAE 68 Deleting Wavelengths and Factors cele 69 Taking NLeasutebietils e sharp utede ce see chee Ghee dica en bak ee di ah 69 Absorbance Ratio oer
16. an empty cell holder as the blank Measure the standards in the order they are listed on the test screen You can measure the wavelength standard at only five wavelengths Torun the Wavelength Accuracy Standards test Highlight Wavelength Accuracy Standards and press Enter Performance Validation 13 33 1Feb02 Wavelength Accuracy Standards Expected Tolerance Measured Result nm nm nm 399 0 2 525 0 2 Place Standard 1 in position 1 Press Start Test or Press ESC to save test To add a wavelength 1 Press Add nm and enter the wavelength value in the Entry field 2 Press Add nm again to add the wavelength to the list 3 Enter the tolerance for the entered wavelength in the Entry field 4 Press Enter To delete a wavelength 1 Highlight the appropriate wavelength 2 Press Delete nm Torun the test 1 Verify that the wavelengths and tolerances are set correctly 2 Press Start Test The results indicate pass or fail for each wavelength 102 GENESYS 10S UV Vis User Guide Thermo Scientific 21 Performance Verification Wavelength Repeatability If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Make sure the target and tolerance values you entered for the calibrated wavelength are the same as the values on the calibration certificate for the standard Wavelength Repeatability This test measures the ability of the spectrophotometer to
17. are preset and cannot be changed The cell holder should be empty Torun the Wavelength Accuracy Internal test 1 Highlight Wavelength Accuracy Internal and press Enter 2 Press Start Test The results indicate pass or fail for each wavelength Performance Uerification 16 13 2Mar0 avelength Accuracy Internal Expected Tolerance Measured Result inm nm nm 47 5 1 248 0 Pass 29 0 1 529 3 Pass 24 2 1 824 4 Pass ake sure the Blank position is empty ress Start Test If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Contact technical support for more troubleshooting advice Wavelength Accuracy Standard Thermo Scientific This test measures the absorbance of a wavelength accuracy standard and compares the location of the peaks with precisely known values at up to five wavelengths By default wavelength accuracy tests are performed in absorbance mode however this test may also be performed in T mode Typical wavelengths and tolerances are included in the firmware but these values can be changed to match the calibration certificate included with your standards GENESYS 10S UV Vis User Guide 101 21 Performance Verification Wavelength Accuracy Standard When running the Wavelength Accuracy test remember You need Wavelength Accuracy standards designed to measure the wavelength accuracy at the specified wavelengths Use
18. d Standard Cnivers cuv iet eaeh Rated x ge tp deep eatis qe 87 Kinetiese RIDE den d Reged aha aie Fani aha rete IR RICE OE ae a 91 Recalling d Leste tain edtoporato p RO LR gal dep oM oer UE ORARE ica Qa ra 92 Setting Up Test Paranielefe 22x ss ede e rere oed renes ela e qd See Seals 92 Measuring Samples ei uoe aiee refe E Red pc por Eae date Ve dar laden 93 Recalling and Recalculating Graphical Kinetics Results s s 94 Rescaling and Recalculating Tabular Kinetics Results 004 97 Performance Verification icc oo ce iru y rea ea d Rc d saca da 99 Accessing the Performance Verification Tests ssc ei tee e Sh ahh ee 99 Troubleshooting Checklist 152 221 backs zie dates exa ee eee Ne 100 Wavelength Accuracy Internal i 5e pete tree Laveen athe dae baa 101 Wavelength Accuracy Standatdu sa sew se oL ten bees bebe ee au 101 Wavelength Bepeatabllisjiei ee addas d REEL RE EM E PE 103 Resolutions oco sa o bE RAR See CEPI ER LINE RU EM Ee 16 104 Photonieiric Accuracy aod oot Let tede evi RE ee ew May 104 Selecting the Made ose abet na bes VUE bee M tr M o ace 105 AddingStandardi o ome ad Wer ne Oe eer a c dne bout Mele qus ita Ter MA 105 Dele ng Standards ae deben poat estados eat bri sty eB d ol esa E pU 106 Running the Testisi mese Coast pa Force Exi She EE Sew VES 106 INOISe s ttu tA SV exo Arata heard O af d ALD O ae E dears aare cds 107 Stray La Git 1h 98 ree tpsa i b pel eb ED ae Role ERA ae eRe EC Er RUP E d ag 108 Runnin
19. during all subsequent tests if Cell Correction is activated Specifying Wavelengths for Discrete nms Mode To specify wavelengths for Discrete nms mode 1 Highlight Sample Positioner and press Enter to set this parameter to either Auto 3 when using three large cell holders or Auto 6 when using six small cell holders 2 Highlight Number of Matched Cuvettes and press Enter 3 Specify the number of cells you are matching and press Enter Cell Correction 3 12pm 24Jan01 Correction Mode Discrete nms Date Corrected Entry Enter Number of Samples 1 5 4 Press Set nms to select the wavelengths for which Cell Correction will be run A list of wavelengths appears Thermo Scientific GENESYS 10S UV Vis User Guide 29 5 Cell Correction Cell Correction Cell Correction 15 33 26Jun02 BLANK nm 1 600 2 540 3 450 Entry Enter Wavelength 190 1100 Note Match cells at all analytical wavelengths Matching at one does not guarantee matching at others 5 Highlight the position where you want to enter the first wavelength 6 Press Add nm 7 Enter the value for the wavelength and press Enter 8 After entering all the wavelengths press Run Corr to start Cell Correction The application measures the other cells against the blank and records stores and dates the measurements From these measurements the application establishes the required correction factors which are applied during all subsequent
20. i st d acr eut id epa KE aee eR Qood 39 Creating Custom US sess Ears Ghar oS eh a Sev Gre phu d 40 Calculator Function ae oda RERO e dites Ro ca od wc Aic o a n Abs and T Measurements Basic A 1 C Luueueeseeeeee 43 Setting the Wavelenpthis oio a uhh asd vce ae Erdgas arai dec cda des aap ae 43 Messurle a Blank dunt tq eto eee race aA Bate den 44 Measuring Samples tiga 32er Qatbesxi Qoi b een arse Vea ed Ed 44 Abs and T Measurements Advanced A T C 0cee cess 45 Recallins a Testre aahei ana ae ccr 45 Setting Up Test Parameters siida m E ete ce e RUP tale hang a a 46 Taking Medasuremefits io cessaret oae Doo nunne d REP MER EA E E 46 Basic Concentration Measurements Basic A oT C LLe 49 Basic Concentration Measurements lees eee 49 Setting the Wavelength and Mode v ioc se eek ce p b E baci a 50 Using Conc Std to Measure Concentration 0 0 0 eee eee eee 50 Using Conc Factor to Measure Concentration 0 0 0 0 ee eee eee 51 Measuring Samples co 0 roseo etra Perrin ed dup as bea pied teases 52 Thermo Scientific Thermo Scientific Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 17 Chapter 18 Contents Concentration Measurements Advanced A T C 00seeeeeee 53 Recallins a Testisi odo S boue leri oeae iia Eaa ac cabe tees atea te sk ed 53 Setting Up Test Parameters ouod ebbe M EXP Wie Ei an We al s a 54 Measuring Standard sien anaes
21. in cell position 1 The instrument always uses cell position 1 to scan the sample 4 Toscana sample 1 Press Run Test If a 6 Position Cell Holder is installed place the sample in cell position 1 2 Press Measure Sample Note To switch between tabular and graphical displays press Graph Tabular Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Thermo Scientific GENESYS 10S UV Vis User Guide 59 14 Scanning Viewing and Manipulating Scan Data Scanning 13 28 20Jun0Z Test Name Filter BLANK 0 730 Wave length 500 IDE 1 Baseline collected 20JunO Baseline p Sanple Viewing and Manipulating Scan Data The Scanning application lets you view and manipulate results in graphical or tabular form When working with graphical scan data press Edit Graph before performing other functions on the data The edit graph screen provides these capabilities e Rescaling Graphical Scan Data Determining Peak Height Using a 3 point Net Equation Performing Calculations on the Scan Data Labeling Peaks and Valleys Rescaling Graphical Scan Data You can modify the scale of your scan data plot automatically or manually When you select Auto Scale the instrument scales the X and Y axes so all the data appears on the plot When you select Manual Scale you select specific minimum and maximum values for the axes When you modify the scale the inst
22. pair 3 Press Add nm 4 Enter the values for the wavelength and factor and press Enter 5 When the values are correct press Add nm 6 Continue until you have entered all the wavelengths and factors Deleting Wavelengths and Factors To delete wavelengths and factors 1 Press Set nms Note If no wavelength values have been entered the wavelength and factor columns will be empty 2 Highlight the first wavelength and factor pair to delete 3 Press Delete nm Taking Measurements You can access Multiwavelength acquisition from either the Set nms screen shown above or from the Multiwavelength setup screen To take measurements automatically Auto 6 or Auto 3 1 Press Run Test to display the Multiwavelength measurement screen Thermo Scientific GENESYS 10S UV Vis User Guide 69 15 Multiwavelength Taking Measurements Multiwavelength 4 46pm 18Feb02 Test Name BLANK IDH 1 Result nm Abs Insert sample 1 into position 1 Press ENTER to measure LL 1 1 Install the blank and samples Press Enter To take measurements manually using Manual 6 or Single Cell Holder With the Multiwavelength screen displayed and the parameters set press Run Test Install the blank and samples If a 6 Position Cell Holder is installed place the blank in the B position The holder can hold five samples Press Measure Blank If a 6 Position Cell Holder is installed it
23. recall atest 1 In the Absorbance Ratio screen see Using the Absorbance Ratio Screen on page 76 press Stored Tests Thermo Scientific GENESYS 10S UV Vis User Guide 75 17 Absorbance Difference Setting Up Test Parameters 2 Highlight the test to recall and press Enter Using the Absorbance Ratio Screen Use this screen for Setting Up Test Parameters e Cell Correction Saving a Test Viewing the list of stored tests Measuring a Blank Measuring Samples Setting Up Test Parameters To set up test parameters 1 In the Absorbance Ratio screen highlight the parameter to set 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples 76 GENESYS 10S UV Vis User Guide Thermo Scientific 17 Absorbance Difference Measuring Samples Measuring Samples Thermo Scientific Absorbance Difference 13 29 14Feb02 Test Name TEST 9 BLANK Abs Abs Abs Result IDH 429 0 630 0 Ref WL ppm Insert samples 1 5 into positions 1 5 Press ENTER to measure L1 1 1 Jj To measure samples automatically using Auto 6 or Auto 3 1 In the Absorbance Difference screen press Run Test 2 Install the blank and
24. tests if Cell Correction is activated 30 GENESYS 10S UV Vis User Guide Thermo Scientific LLL m Managing Stored Tests The instrument uses test method files containing the values for all parameters needed to run a test including those for cell changer alignment and installed accessories After setting the parameters you can assign a unique test name and save the test You can then restore the test and run it without having to set the parameters again When you power down the instrument the current test is maintained by battery backup When you turn the instrument on again the cell holder alignment and values for all parameters are the same as when the instrument was last used If you load a saved test the values for all parameters stored with it replace the current values of the test parameters Software Password This password lets you lock test setups test parameters so they may not be overwritten or deleted The password also lets you remove the security so you may edit the test parameters See the Lock Unlock section for more information on locking a test Note This password cannot be changed Password 4363797 Note Tests stored on a USB memory device cannot be locked or unlocked Naming a Test When saving a test specify its file name using up to eight alphanumeric characters To name a test 1 After setting the test parameters highlight Test Name and press Enter Thermo Scientific GENESYS 10S
25. 15Feb02 Photometric Accuracy H nm Expected Tol Meas ZT ZzT zT Result 1 590 0 10 1 1 2 10 1 Pass 2 590 0 9 4 1 2 9 4 Pass Place Standards 1 2 in positions 1 2 Press Start Test or Press ESC to save test to Abs Std Std Test If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Follow the guidelines provided with the standard reference materials For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us 106 GENESYS 10S UV Vis User Guide Thermo Scientific Noise Thermo Scientific 21 Performance Verification Noise This test measures the amount of noise at 340 nm All test parameters are determined by instrument specifications and cannot be changed by the user When running the noise test remember Perform the 0A measurement with the cell holder empty Optionally you can perform the 2A measurement with a 2A filter To run the Noise Measurement test Highlight Noise Measurement Press Enter Performance Ualidation 11 54 15Feb02 Noise Measurement eon Peak to Peak Meas Result Tolerance Abs 230 0nm 50 002 0 000 Pass 340 0nm 50 001 0 000 Pass e 2A Peak to Peak Meas Result Tolerance Abs 230 0nm 50 003 0 000 Pass 340 0nm 50 002 0 000 Pass Make sure the Blank position is empty Place 2A filter in Cell Position 1 Press Start Te
26. Configurations The following table shows the available cell holders and their accessories You can install or remove accessories without switching off the instrument Cell Changer System Single Cell System Standard Cell Holders Thermo Scientific GENESYS 10S UV Vis User Guide 15 3 Accessories Cell Holders and Cell Holder Accessories Cell Changer System Single Cell System Accessory Cell Holder Systems Single Cell Recirculator Temperature Control t O Must be installed in the B 2 and 4 positions Test Tube Holder Must be installed in the B 2 and 4 positions 50 mm Rectangular Long Pathlength Cell Holder Daad 2 Must be installed in the B 2 and 4 positions 50 mm Cylindrical Long Pathlength Cell Holder O333 Os Must be installed in the B 2 and 4 positions 16 GENESYS 10S UV Vis User Guide Thermo Scientific 3 Accessories Cell Holders and Cell Holder Accessories Cell Changer System Single Cell System 100 mm Rectangular Long Pathlength Cell Holder Cannot use 100 mm path cells with Cell Positioner 100 mm Cylindrical Long Pathlength Cell Holder Cannot use 100 mm path cells with Cell Positioner CS gt Thin Film Filter Holders Sse Os Must be installed in the B 2 and 4 positions Adjustable Filter Lens Holder Must be installed in the B 2 and 4 positions Thermo Scientific GENESYS 10S UV Vis User Guide 17
27. NESYS 10S UV Vis User Guide vii Preface viii GENESYS 10S UV Vis User Guide Thermo Scientific LLL EB Spectrophotometer Basics This chapter describes Spectrophotometer Components Cell Holders Selecting and Positioning Cuvettes e Z dimensions Spectrophotometer Components Here are some major components visible on the outside of a typical instrument Sample compartment USB port Optional printer housing Keypad Connectors The connectors are on the back of the instrument Thermo Scientific GENESYS 10S UV Vis User Guide 1 1 Spectrophotometer Basics Spectrophotometer Components USB communication port USB printer port Fuse compartment A C power connector On Off switch WARNING Avoid shock hazard Always turn off the instrument and unplug it from the wall outlet or power strip before you unplug the power cord from the instrument connector 2 GENESYS 10S UV Vis User Guide Thermo Scientific About the Keypad Thermo Scientific Test Name Wavelength 1 Wavelength 2 Factor 1 Factor 2 Units anple Positioner Number of Samples 2 parameters fibsorbance Difference f Mavelength Correction Press f or to select item to change ave ore 12 500 3Jan 1 Spectrophotometer Basics Spectrophotometer Components Test Key or button 0000 Function keys Tests un Test Function Called Function key
28. NK Test Name BILIRUBIN avelength 1 545 Onm Wavelength 2 546 Onm Wavelength 3 547 Onm Factor 1 000 Units C Sample Positioner Auto 6 Number of Samples 1 More parameters Press f or to select item to change Test Teste Test Test Tests Test Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Thermo Scientific GENESYS 10S UV Vis User Guide 79 18 3 Point Net Recalling a Test Recalling a Test To recall a test 1 Press Stored Tests 2 Highlight the test to recall and press Enter to display its parameters This screen provides these capabilities Setting Up Test Parameters Cell Correction Saving a Test Viewing the list of stored tests Taking Measurements Setting Up Test Parameters To set up test parameters 1 Highlight the desired parameter 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Taking Measurements To take measurements automatically Auto 6 or Auto 3 1 With
29. Name FILTER BLANK Wave length Abs 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 IDH 1 Baseline collected 15Feb02 da em x data To use all the scan data With your table of scan data displayed on the edit screen press Use All Data To select specific start and end wavelengths 1 With your table of scan data displayed on the edit screen highlight the appropriate data point in the table 2 Press Start nm or End nm The instrument highlights the selected data points To display the plot using the highlighted data points press Graph 66 GENESYS 10S UV Vis User Guide Thermo Scientific gt A Multiwavelength The Multiwavelength application lets you make multiple fixed wavelength measurements It is a fast alternative to scanning if the wavelengths of interest are well known Use Multiwavelength for e Recalling a Test Setting Up Test Parameters Cell Correction Adding Wavelengths and Factors Deleting Wavelengths and Factors Taking Measurements To get started press Test highlight Multiwavelength and press Enter Multiwavelength 10 30 22Feb0 BLANK Measurement Mode Absorbance Sample Positioner Auto 6 Number of Samples s Cell Correction Off ID O OFF 1 Press f or to select item to change ave ored Set ms Tost tests Recalling a Test To recall atest 1 Press Stored Tests 2 Highlight the test to recall and press Enter to display its parameters
30. Page 1 Standards 1 5 Em D ES Basal Blank Test Standards Set Entry concentration Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 5 Press Measure Standards GENESYS 10S UV Vis User Guide 85 19 Concentration Measurements Standard Curve Application Measuring Samples If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the standards manually When all of the standards have been measured the Standards screen see Using the Standards Screen on page 86 shows the absorbance of each standard along with the slope intercept and correlation coefficient of the standard curve Using the Standards Screen Here is an example of the Standards screen showing measurement results Standard Curve 15 50 4Feb02 Test Name AMMONIA Cell 5 Std Conc Abs ppm 660 Onm 1 0 120 0 087 2 0 200 0 140 3 0 250 0 152 4 0 400 0 268 5 0 500 0 312 Curve Fit Linear Slope 0 6091 Std Dev 0 011 Intercept 0 01272 Corr Coeff 0 995 Page 1 Standards 1 5 HS p ae p Test Standards Test You can use this screen for Displaying a graph of the standard curve data press View Graph Saving a Test press Save Test Editing a Standard Curve press Edit Standards Measuring Samples press Run Test Measuring Samples To measure samples
31. SB port on the back of the instrument see Connectors on page 1 Note The instrument is compatible with most HP PCL printers brands other than HP will also be supported If the printer is not purchased from Thermo Scientific it is your responsibility to determine if the printer is compatible with the instrument Contact technical support or your sales representative for more information Thermo Scientific GENESYS 10S UV Vis User Guide 23 3 Accessories External Printers 24 GENESYS 10S UV Vis User Guide Thermo Scientific oo A Sample Positioner Setting The spectrophotometer lets you use many different cell holders and accessories to take measurements When you select your test parameters you select the type of measurement required and indicate the number of samples You can choose from the following measurement options Auto 6 Measure one blank and up to five samples without changing cuvettes in the cell changer The instrument automatically measures the blank and advances the cell changer to measure the remaining samples Auto 3 Measure one blank and two samples without changing cuvettes in the cell changer The instrument automatically measures the blank and advances the cell changer to measure the samples in positions 2 and 4 Single Cell Holder Place the blank in the cell holder measure it place a sample in the cell holder and then measure your sample This process is completely manual The cell positi
32. Scanning Test Name Lets the user enter an alphanumeric name maximum of 16 characters for the test the name will be included on the data printout and if the test is saved will be displayed in the Utility Test Directory screen available in all tests Tm value Calculates the melting temperature Oligos tests Total Run Time GENESYS 10S UV Vis User Guide Enters the time from the Run initiation to the end of the test equals Delay Time Interval Times Measurement Times Kinetics Thermo Scientific 23 Parameters Parameter Description Units Selects or creates units labels for results all stored tests except Abs Ratio Scanning Cell Growth Unselect Test Removes the gt tag of the highlighted test name to remove the test from the SmartStart menu Utility Stored Tests Directory Wavelength Enters values for the analytical wavelengths Thermo Scientific GENESYS 10S UV Vis User Guide 125 23 Parameters 126 GENESYS 10S UV Vis User Guide Thermo Scientific ng Calculations for Software Calculation Calculation s Graphs Standard Curves Partial sums SX F Where x Concentration of i standard y Absorbance of i standard N number of standards Linear regression A A c general case Where A absorbance c concentration A c is defined by an equation of the form A c agc a c a c7 a C ag Where ag Y axis intercept a a4 coeffici
33. an Speed Interval Cell Correction IDH O OFF uto Print uto Save Data Setting Up Test Parameters itN To set up test parameters 1 Highlight the desired parameter 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or a sample Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Collecting a Baseline Scan Note If a 6 Position Cell Holder is installed be sure to place the blank in the B position The instrument always uses the B position to collect the baseline itN To collect a baseline scan 1 Press Run Test 2 Place the blank in the B position 58 GENESYS 10S UV Vis User Guide Thermo Scientific 14 Scanning Scanning a Sample Scanning 15 30 14Feb02 Test Name Cell 1 3 000 A 0 400 0 Wave length 500 0 IDit 1 Baseline Tabular Baseline 3 Press Collect Baseline While the spectrophotometer is measuring the baseline a status message appears indicating the progress of the baseline scan After the baseline is measured this message disappears Note To switch between tabular and graphical displays press Graph Tabular Scanning a Sample Note If a 6 Position Cell Holder is installed be sure to place the sample
34. an authorized service representative to perform any service procedure you do not feel comfortable performing Routine Care Routine care for the spectrophotometer does not require a lot of time To help minimize maintenance time and increase the life and performance of your instrument please follow these guidelines Always replace the dust cover when the instrument is not turned on to prevent dust from accumulating in and on the instrument Do not use or store the instrument in a corrosive environment Gently wipe the outside of the instrument including the keypad with a soft cloth to remove any dust or spills Water isopropyl alcohol and other common laboratory cleaning agents may be used if necessary Always clean up spills as soon as they occur to prevent or minimize damage to the instrument If concentrated acids or bases or any hydrocarbon materials are spilled on the instrument clean up the affected area immediately Thermo Scientific GENESYS 10S UV Vis User Guide 111 22 Maintenance Routine Care Cleaning and Maintaining Cells Carefully check the condition of the cuvettes and other cells used to measure samples If they are chipped cracked or scratched it is important to discard the damaged cell s and replace them with new ones Ensuring your cuvettes are clean both inside and out is important to the quality of your results for two reasons 1 contaminating material may absorb light resulting in falsely hig
35. ance value Accept Name Accepts the displayed Name entry Test Name and Edit Units Add Character Adds a highlighted character to the Name entry Test Name and Edit Units Add nm Lets you add a wavelength and factor to the list in Multiwavelength tests and some Performance Verification tests Area Lets you calculate area under the peak in the graph Scanning AutoPrint Turns the automatic printout on or off Autoscale Rescales the graph to the original ranges of the X and Y axes Kinetics Scanning Base Sequence Sequence of bases contained in the sample Oligos calc factor tests GENESYS 10S UV Vis User Guide 117 23 Parameters 118 Parameter Baseline Expiration Description Enters the time when the baseline for scan tests will need to be collected again Utility Beeper Turns the audible signal for key presses on and off Utility Calculation Baseline Selects the zero baseline or the tangential baseline to calculate area under the peak in the graph Scanning Calculator Enables the calculator mode Utility Cell Correction Selects the option to automatically correct for variances in absorption between the cuvettes all test types Cell Position Displays the position placed in the light path only with auto turret Change Mode Switches measurement modes Change to Abs Basic A T C and some Performance Verification tests Change to T Collect Ba
36. ating Graphical Kinetics Results The Kinetics application lets you view and manipulate results in graphical or tabular form When your results are displayed you can modify the range start and stop time and the instrument recalculates the reaction rate When working with graphical kinetics results you need to press Edit Graph before you can rescale and recalculate 94 GENESYS 10S UV Vis User Guide Thermo Scientific Thermo Scientific 20 Kinetics Recalling and Recalculating Graphical Kinetics Results Kinetics 3 29pm 18Feb02 Test Name ENZYME linm m 1 850 Aa 0 840 Time 3 00 IDit Abs min Result 1 0 251 1756 Cursor Scale Scale Data You can modify the scale of your kinetics data plot automatically or manually When you select Auto Scale the instrument automatically scales the X and Y axes so all the data appears on the plot When you select Manual Scale you select specific minimum and maximum values for the X and Y axes Whenever you modify the scale the instrument recalculates and displays the new reaction rate and result The edit screen lets you Use Auto Scale to change the scale display the new graph and recalculate the results Use Manual Scale to change the scale display the new graph and recalculate the results Use the cursor to select new minimum or maximum values for the X axis and recalculate the results To use Auto Scale With your kinetics data displayed on the Edit Graph screen press Auto S
37. automatically using the calibration curve using Auto 6 or Auto 3 1 Press Run Test 2 Install the blank and samples 3 Press Enter The Standard Curve screen shows the absorbance and concentration of each sample To switch between tabular and graphical displays press View Graph Tabular 86 GENESYS 10S UV Vis User Guide Thermo Scientific 19 Concentration Measurements Standard Curve Application Editing a Standard Curve Standard Curve 16 05 4Feb02 Test Name AMMONIA BLANK 0 3 A 0 1 ppm Curve Fit Linear Slope 0 6091 Std Dev 0 011 Intercept 0 01272 Corr Coeff 0 995 cese EX ae OE Test Fit Test To measure samples manually using Manual 6 or Single Cell Holder 1 Press Run Test 2 Install the blank and samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 4 Press Measure Samples If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually When the instrument has measured all the samples the Standards screen shows the absorbance and concentration of each sample Editing a Standard Curve You may edit the concentration of any standard on a standard curve In addition you may change the number of standards select a different curve fit or delete points from the curve Thermo Scientific GENESYS 10S UV Vis Us
38. cale The instrument adjusts the minimum and maximum values for the X and Y axes so all the data appears on the plot The instrument also recalculates the results using all the data and displays them To use Manual Scale 1 With your kinetics data displayed on the edit screen press Manual Scale to display the manual scale options GENESYS 10S UV Vis User Guide 95 20 Kinetics Recalling and Recalculating Graphical Kinetics Results 96 GENESYS 10S UV Vis User Guide Kinetics 3 24pm 27Jan01 Test Name ENZYME BLANK 0 740 A 0 583 Time IDit A min Result 1 0 050 22 69 n e e Enter the appropriate minimum or maximum value for the X or Y axis and press Min Y Max Y Min X or Max X to accept it The instrument redraws the plot using the entered minimum and maximum values and displays the recalculated rate and result Continue until you have entered all the values you want to change To use the Cursor function With your kinetics data displayed on the edit screen press Cursor to display the cursor options Kinetics 3 35pm 27Jan01 Test Name ENZYME BLANK 0 740 A 0 583 Time Time Abs 1 30 0 644 n e e Cursor e Cursor gt Press Cursor lt or Cursor to position the cursor line on the appropriate point on the graph The data for the selected point appears When the cursor line is in the correct position press Set Min X or Set Max X to accept the selected point Thermo
39. ce measurement appears on the display 44 GENESYS 10S UV Vis User Guide Thermo Scientific LLL m Abs and T Measurements Advanced A I C Use the Advanced A 96T C application for Absorbance or 96 Transmittance measurements that include Cell Correction or a measurement delay time or for automating the measurement of multiple samples with a cell changer This section covers Selecting the measurement mode Absorbance or 96 Transmittance Cell Correction Recalling a Test Setting Up Test Parameters Taking Measurements To get started press Test highlight Advanced A T C and press Enter Advanced A T C 11 42 4Feb02 Measurement Mode fibsorbance Wave length 546 Onm Delay Time min sec 0 00 Sample Positioner Auto 6 Number of Samples 1 More parameters Press f or to select item to change LER Dum D En Test Tests Test Recalling a Test To recall a test 1 In the Advanced A T C screen press Stored Tests 2 Highlight the test you want to recall and press Enter Use this screen for Thermo Scientific GENESYS 10S UV Vis User Guide 45 11 Abs and T Measurements Advanced A T C Setting Up Test Parameters Setting Up Test Parameters Cell Correction Savinga Test Viewing the list of stored tests Taking Measurements Setting Up Test Parameters To set up test parameters 1 Highlight the desired parameter Some parameters appear only if you select one of the concentration mod
40. cedure above for unselecting a test 38 GENESYS 10S UV Vis User Guide Thermo Scientific ee i Concentration Units This chapter covers Specifying Concentration Units Creating Custom Units Specifying Concentration Units Concentration and kinetics tests include a parameter for units which labels the results The spectrophotometer includes a set of basic concentration units All programs in the spectrophotometer use the same list of basic units C concentration e pg L ppm M L ppb mM L e g L e IU mg L e pM uL mg mL ng uL Custom units can also be created for more information see Creating Custom Units on page 40 To select the units 1 Highlight Units and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 39 8 Concentration Units Specifying Concentration Units Advanced A T C 10 09 14Feb02 Test Name Measurement Mo Wavelength Delay Time mi Conc of Standa Factor nits Sample Positio More parameter Press f or to select Press ENTER Press f or to select item to change al Ooo i deia o O 2 Highlight the unit you want to select and press Enter Creating Custom Units In addition to the basic concentration units you can create a custom concentration unit and add it to the list This custom concentration unit can be changed when desired Note Only one custom concentration unit is available in the list at one time To
41. cientific GENESYS 10S UV Vis User Guide 97 20 Kinetics Rescaling and Recalculating Tabular Kinetics Results 98 GENESYS 10S UV Vis User Guide Thermo Scientific ig Performance Verification Performance Verification lets you check the performance of your instrument with these tests e Wavelength Accuracy Internal e Wavelength Accuracy Standard e Wavelength Repeatability Resolution Photometric Accuracy Noise Stray Light nternal Printer Test Run the appropriate tests regularly and maintain a log of results to help document the reliability of the instrument and indicate potential performance issues Note If a printer is installed and turned on the instrument automatically prints test results You can also press Print to print another copy of the results Accessing the Performance Verification Tests To access the Performance Verification tests 1 Press Tests 2 Highlight Performance Verification and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 99 21 Performance Verification Troubleshooting Checklist Performance Verification 4 21pm SAugd9 Internal avelength Accuracy Standards avelength Repeatability esolution Photometric Accuracy oise Measurement Stray Light Internal Printer Test ress T or to select test Troubleshooting Checklist If a Performance Verification test fails follow the instructions below to diagnose common problems If
42. create custom units 1 With the Units Selection window displayed press Edit Unit Use this screen to Delete the name of a unit Delete a character in the name of a unit Add a character to the name of a unit Accept the name of a unit 2 Follow steps 2 through 4 in Naming a Test on page 31 The new custom unit appears in the list of basic units 40 GENESYS 10S UV Vis User Guide Thermo Scientific MEN uU Calculator Function To use the Calculator function 1 From the Utility menu highlight Calculator and press Enter 2 Use the numeric keypad to enter the desired value 3 Press the desired function x or 4 Enter the second desired value and press Enter Note You can only add subtract multiply or divide two lines of numbers at a time tility 9 23am 14Mar07 Software R Screen Con Printout C Stored Tes Thermo Scientific GENESYS 10S UV Vis User Guide y 9 Calculator Function 42 GENESYS 10S UV Vis User Guide Thermo Scientific S E Abs and T Measurements Basic A I C Basic ATC mode puts the instrument into an instant measurement mode The user simply walks up to the instrument inserts a sample and measures it Depending on whether the mode is set to Absorbance A Transmittance T or Concentration the result appears along with the type of measurement the date and time the wavelength and the cell position used for the measurement To toggle between Abso
43. curacy Ht nm Expected Tol Meas zT zT zT Result A 1 590 0 50 000 1 200 2590 0 10 000 1 200 Place Standards 1 2 in positions 1 2 to Abs Std Std Test To switch between absorbance and 96 Transmittance press Change to T or Change to ABS until the appropriate mode appears Adding Standards You will need to set three values whenever you add a standard the wavelength the absorbance or 96 Transmittance and the tolerance value WN wd OB Thermo Scientific To add a standard Press Add Std and enter the wavelength value in the Entry field Press Enter or Add nm to add the wavelength to the list Enter the absorbance or T value for the entered wavelength in the Entry field Press Enter Enter the tolerance for the entered wavelength in the Entry field Press Enter GENESYS 10S UV Vis User Guide 105 21 Performance Verification Photometric Accuracy The test screen displays the values you just entered for that standard 7 Press Start Test to begin the measurement or Press Esc to save the test Deleting Standards To delete a standard 1 Highlight the appropriate standard 2 Press Delete Std Running the Test In the Photometric Accuracy screen make sure the wavelengths absorbance or T values and tolerances are set correctly Torun the Photometric Accuracy test Press Start Test The results indicate pass or fail for each wavelength Performance Validation 11 44
44. cure it to the baseplate by tightening the captive screw Note You can install only three each of some accessory cell holders Be sure to place them in positions B 2 and 4 Note Remove the baseplate from the instrument before removing or installing cell holders See Installing the 6 Position Cell Holder and the Single Cell Holder on page 19 for instructions on installing the complete accessory assembly in your instrument Installing the Internal Printer Optional CAUTION Avoid shock hazard Turn off the instrument and disconnect the power cord from the outlet before installing the internal printer Captive screw on lamp door Removing door from hinge Connecting wires to printer Hinge Thermo Scientific GENESYS 10S UV Vis User Guide 21 3 Accessories Installing the Internal Printer Optional WN A OG 10 11 To install the internal printer Loosen the captive screw on the lamp door by rotating it counterclockwise about turn Open the lamp door Usea pen or screwdriver to lift the tabs holding the door to the hinge Slide the door off the hinge Remove the printer assembly printer installed on the accessory door from its packing Lower the hinge so it is out of the way Connect the wires and press into place with a small screwdriver There is only one way that the connectors will fit Each connector has a slight D shape Make sure the side of the connect
45. data acquisition Frequently used in enzymatic kinetics a factor can be multiplied by the slope of the linear rate fit to determine activity Computer software control lets you greatly expand the kinetics capabilities of your instrument Multicell parallel kinetics lets you monitor up to five reactions simultaneously Extended kinetics data acquisition exceeds the 400 data point limit of the embedded software Software control streamlines the use of more sophisticated computer applications to analyze kinetics data after collection Use Kinetics for Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples Recalling and Recalculating Graphical Kinetics Results e Rescaling and Recalculating Tabular Kinetics Results Modifying the scale of the plot You can work with graphical or tabular data and perform the same functions with either However the location of the function keys depends on the display type Note The Kinetics application lets you measure only one sample at a time Note The Kinetics application lets you collect up to 400 data points per run When setting test parameters select the interval time and total run time accordingly To get started press the Test button highlight Kinetics and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 91 20 Kinetics Recalling a Test Recalling a Test To recall a test 1 In the Kinetics screen pre
46. de Thermo Scientific 13 Concentration Measurements Advanced A T C Entering a Factor 4 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 5 Press Measure Standard The instrument measures the absorbance of the standard and displays the absorbance and calculated factor Entering a Factor To enter a factor Highlight Factor To change the factor enter the correct factor To change the units highlight Units and select the correct units Measuring Samples To measure a sample automatically using Auto 6 or Auto 3 1 Press Run Test 2 When prompted place the blank and samples in their cell positions and press Enter The instrument measures the blank and samples and displays the sample measurements 3 Press Measure Sample to measure additional samples To measure samples manually using Manual 6 or Single Cell Holder 1 Press Run Test 2 Insert the blank and sample A 6 Position Cell Holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 4 Press Measure Sample If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually Thermo Scientific GENESYS 10S UV Vis User Guide 55 13 Concentration Measu
47. dete o PIE uk Spe pur Ead Re Cau PE aa das n Recalling d Testr oia aote sonics Ryo aca s Baa Paci Pace Pad eda Eo Aerea 71 Setting Up Test Parameters caus hens nw preted seeped Se weg op eee 72 Measuring Sabiples cud cci eus Shares twee ee ote bd oor eat dt 72 Absorbance DifferBnee ue pn or E eror a m dod Ex e ha 75 Recalline d lest 012 Gl es aie Ss E 2292 Bae ak dabo baat a Roos 75 Using the Absorbance Ratio Screens i dapat aioe weed aed 76 Setting Up Test Patdnieloreo ix aerae senden cer oru vienes Seal eas ln 76 Measuring Samples sos cueste kate x eed s att ete oett A 77 J POIRIENGE oo errare X e greci n a a etre ROO E TQ Ker x RA ON OR a 79 Recalling a Vest 2 curb OPERE Eu bela do d deal Edo A edP Deor Miles 80 Setting Up Test Patanielefes eq ateaoses en etddeatceu gae biete e poat Veg Mosen 80 Taking Measurements 13 rotor Ero xir Ra E EQ WARE edad 80 GENESYS 10S UV Vis User Guide V Contents Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Chapter 24 Chapter 25 vi GENESYS 10S UV Vis User Guide Concentration Measurements Standard Curve Application 83 Recalling a Standard Curve uade loa tecta ve ded ce et eed talents dla ens 84 Setting the Parameters for a Standard Curve dd Dd d ra 84 Measuring the Standards for a Standard Curve 0 0 00 eee eee ee 84 Usine the Standards Screen 2o i acted d eap E PME M PRAG 86 Measuring Sami plesty e es os Era EYES NGC Lee AC cau NUS 86 Ediung
48. diting a Standard Curve All absorbance measurements are removed from the screen To reset standards 1 With the standard curve displayed press Edit Standards 2 Highlight Reset Standards and press Enter All standards and measurements are removed from the screen To select a different curve fit for a standard curve Note To change the curve fit for a standard curve you must display the standard curve as a graph not as a table 1 With the standard curve you want to edit displayed as a graph press Change Fit Standard Curve 16 21 4Feb02 Test Name AMMONIA BLANK 0 3 Linear Through Zero Linear Regr 2nd Order Linear Regr 3rd Order A Linear Regr 4th Order Segmented Press fT or to select Press ENTER 0 1 ppm 0 538 Curve Fit Linear Slope 0 6091 Std Dev 0 011 Intercept 0 01272 Corr Coeff 0 995 L1 1 1 Jj 2 Highlight the curve fit to use for the standard curve and press Enter The instrument applies the selected curve fit to the data and displays the new fit Thermo Scientific GENESYS 10S UV Vis User Guide 89 19 Concentration Measurements Standard Curve Application Editing a Standard Curve 90 GENESYS 10S UV Vis User Guide Thermo Scientific oo Kinetics The Kinetics application lets you measure the change in the sample absorbance as a function of time The local control software allows the determination of a linear rate over a particular region which can be defined after the
49. e warranty is void by the manufacturer GENESYS 10S UV Vis User Guide 113 22 Maintenance Changing the Fuse IMPORTANT Do not dry cells in an oven Micro flowcells can be kept clean by Flushing well with a solvent after use e Aspirating dilute acid base non filming detergent or Clorox through the cell in short bursts Storing with distilled water in the cell Cleaning the Windows of the Sample Compartment Do not use acetone or abrasive materials to clean the windows of the sample compartment Instead use a non abrasive laboratory cleaning solution such as a commercial cell cleaning solution distilled water or alcohol Use the liquid and a soft lint free cloth to clean the windows Do not apply too much pressure or the surface of the windows may be damaged Be sure to remove all fingerprints Changing the Fuse The fuse is located in the power entry module located at the center of the back panel of the instrument 120 VAC 2 5 A Slo Blo e 240 VAC 1 25 A Slo Blo 2 required IMPORTANT The instrument fuse must be replaced with the same type and rate IMPORTANT If the fuse fails repeatedly it may indicate a serious problem with the instrument Contact technical support as soon as possible To change the fuse 1 Turn off and unplug the instrument from the wall outlet or power strip 2 Position the instrument so you can access the power entry module on the back of the instrument
50. e Single Cell Changer is installed remove the blank and place the standard in the cell changer Oo Press Units Standard to set the units and measure the standard A Press Enter Conc enter the concentration value of the standard and press Enter 5 Press Select Units highlight the appropriate unit in the list and press Enter 6 Press Measure Standard The instrument measures the absorbance of the standard and displays the absorbance and calculated factor Using Conc Factor to Measure Concentration In this mode a concentration factor is used to determine the concentration of samples The concentration of unknown samples is determined ratiometrically from the entered factor The measurement can be expressed mathematically as Abs T of Sample Factor Concentration of Sample where the factor is entered the Abs T of the sample is measured and the sample concentration is calculated To use Conc Factor to measure concentration 1 If necessary press Change Mode to switch to the Conc With Factor mode 2 Press Units Factor to set the factor and select the units Conc with Factor 15 29 6Mar01 BLANK Factor 45 Units C Press f or to select item to change EE Du Factor Units 3 Press Enter Factor 4 Type the desired factor value Thermo Scientific GENESYS 10S UV Vis User Guide 51 12 Basic Concentration Measurements Basic A T C Measuring Samples 5 Press Enter Factor to accept the factor and
51. e standards See Parameters on page 117 for a complete list a Enter the Test Name Wavelength Reference Wavelength Correction and Reference Wavelength b Select the Curve Fit Units and Sample Positioner c Set Number of Standards and Number of Samples d Enter the low and high limits e Select the settings for Statistics and AutoPrint f Run Cell Correction Measuring the Standards for a Standard Curve To measure standards automatically using Auto 6 or Auto 3 1 Install che blank and standards 2 When the parameters are correct press Run Standards 84 GENESYS 10S UV Vis User Guide Thermo Scientific Thermo Scientific 19 Concentration Measurements Standard Curve Application Measuring the Standards for a Standard Curve Standard Curve 15 39 4Feb02 Test Name AMMONIA BLANK Std Conc Abs Entry Enter a number 0 001 to 9999 Set Entry concentration and press Enter Press Measure Standards The Standards screen shows the absorbance of each standard along with the slope intercept and correlation coefficient of the standard curve To measure standards manually using Manual 6 or Single Cell Holder Install the blank and standards When the parameters are correct press Run Standards Standard Curve 12 50 14Feb02 Test Name AMMONIA BLANK Std Conc Abs mg L 660 Onm 1 25 00 2 45 00 3 55 00 4 75 00 5 100 0
52. e test Use the procedure in Naming a Test on page 31 GENESYS 10S UV Vis User Guide 33 6 Managing Stored Tests Loading Test Files Create Test Name 1 39pm 7Augd9 ABCDEFGH I JKLMNOPQRS TUVWX YZ0123456789 labcdefghijklmnopqrspuvuxyz _ pC tt Test Name Cobalt Press T or to select character Press Accept Name to save the test Breta Name Character Character Name 4 Press Accept Name 5 Select whether the test is included as a SmartStart test See SmartStart on page 37 Create Test Name 1 42pm 760ug09 Include as a SmartStart test Do not include as a SmartStart test Press fT or to select Press ENTER Press ESC to exit ress T or to select character ress Accept Name to save the test 6 Highlight the appropriate SmartStart option and press Enter to save the test Loading Test Files You can load saved test files from the internal memory from the Utility screen To load test files 1 To access all test files press Utility 2 Highlight Stored Tests Directory and press Enter 34 GENESYS 10S UV Vis User Guide Thermo Scientific Lock Unlock Utility Test Directory DNA 260 230 dsDNA ssDNA RNA Oligos calc factor Direct UU 260 Protein Tests Bradford Standard Bradford Micro Louru Standard ore tests DNA with Scan 260 280 DNA with Scan 260 230 Oligos Centered factor ress T or to select test Read onl Read only Read only
53. ection is now activated as indicated by the word On Note When Cell Correction is activated additional parameter lines are added to the screen above the Cell Correction line If the Cell Correction line is no longer visible highlight More Parameters and press Enter 4 Highlight Setup Correction and press Enter 5 Highlight Correction Mode and press Enter to set the mode to either Scan Cell Correction is run on a blank and one sample cell for the range of wavelengths you specify in Scanning mode Discrete nms Cell Correction is run on a blank and up to five sample cells for up to 31 user specified discrete wavelengths Cell Correction 4 35am 21Jan01 BLANK orrection Mode Discrete nms Date Corrected 1 Sample Positioner futo 6 Number of Matched Cuvettes 1 Press f or to select item to change iun Set we corr 6 If you selected Scan mode in the preceding step specify the Start Wavelength and the Stop Wavelength values 7 Press Run Corr to start Cell Correction 28 GENESYS 10S UV Vis User Guide Thermo Scientific 5 Cell Correction Cell Correction If you selected Discrete nms mode first specify the wavelengths using the following procedures and then Cell Correction Cell Correction measures the other cells against the blank and records stores and dates the measurements From these measurements Cell Correction establishes the required correction factors which then are automatically applied
54. ent zeros and measures sample in same position Not available in Kinetics and Scanning Manual 6 cell changer moved by buttons always zeros on position B then returns to set position to start measurement Not available in Kinetics and Scanning Auto 3 turret auto moved B 2 4 always zeros on position B then goes to position 2 to start measurement Not available in Kinetics and Scanning Auto 6 turret auto moved B 1 2 3 4 5 always zeros on position B then goes to position 1 to start measurement Sample Volume Enters the total volume of sample under Dilution Multiplier in some Bio Tests Save Test Saves all parameters of the current test in internal memory for later recall all tests Scan speed Selects the speed nm min for a scan Slow Medium Fast Scanning tests only Screen Contrast Lets you improve visibility of the display by changing the contrast between the background and text Utility Select Test Tags the highlighted test name with gt to include the test in the SmartStart menu Utility Stored Tests Directory Set Max X Sets the cursor position in the graph as the minimum X and the maximum X values for recalculating rate Set Min X Kinetics Set nms Lets you enter and edit the wavelength and factor values Set Options Selects the factor entry or the baseline to calculate area under the peak in the graph Scanning Setup correction Initiate
55. ents The coefficients are computed using the least squares method Thermo Scientific GENESYS 10S UV Vis User Guide 127 24 Calculations for Software Calculation Linear regression through zero Segmented model Validity of standard curves Calculation s Graphs A a c Where A absorbance c concentration a slope The slope is calculated as a SXY SXX This model requires Slope is not equal to zero or infinity Atleast one standard data point with concentration gt 0 The absorbance of the 0 concentration blank 0A The segmented model requires Data for at least two standard data points with different concentrations and absorbances Slopes of all segments must be ascending positive or descending negative A c gt A c for all c gt c or A A c lt A c for all c gt c Where A absorbance ABSORBANCE MEISURED gt Cj C2 concentration 8 ei E UNDEPNED 3 CONCEN 2 Valid nonlinear standard curve If this is not the case there will be more than one solution within the specified domain and the message Curve cannot be used to determine sample y concentrations it may produce ambiguous results will appear when the curve is viewed 2 MEASURED ABSORBANCE Invalid nonlinear standard curve 128 GENESYS 10S UV Vis User Guide Thermo Scientific Calculation Statistics Linear regression general case Linear regression through zero model
56. er Guide 87 19 Concentration Measurements Standard Curve Application Editing a Standard Curve Standard Cury 11 31am 21Jan01 Test Name STANDARDCURVE Std Conc Edit Concentration ng L Add Standard m aE e Delete Clear Measurements 2 45 00 Reset Standards 3 55 00 4 75 00 Press fT or to select 5 100 0 Press ENTER Curve Fit Linear Slope 0 002877 Std Dev 0 017 Intercept 0 002364 Corr oa 0 985 Page 1 of O Standards Pre 88 GENESYS 10S UV Vis User Gui ss T or to view data To edit the concentration of a standard With the standard curve displayed highlight the standard to edit and press Edit Standards With Edit Concentration highlighted press Enter Press Edit Conc or a number key Enter the concentration value in the Entry field Press Enter To add a standard With the standard curve displayed press Edit Standards Highlight Add Standard Enter the concentration value of the additional standard in the Entry field Press Enter Press Measure Standards to remeasure all the standards To delete a standard With the standard curve displayed highlight the standard to delete and press Edit Standards Highlight Delete Standard and press Enter To clear measurements With the standard curve displayed press Edit Standards Highlight Clear Measurements and press Enter de Thermo Scientific 19 Concentration Measurements Standard Curve Application E
57. es while others appear regardless of the selected measurement mode See Parameters on page 117 fora complete list 2 When the parameters are set press Save Test to save the test or Measure Sample to measure a blank or samples Taking Measurements To take measurements automatically using Auto 6 or Auto 3 1 Press Run Sample 2 Install the blank and samples 3 Press Measure Sample To take measurements manually using Manual 6 or Single Cell Holder 1 Press Run Sample 2 When prompted place the blank and samples into the cell holder If the 6 Position Cell Holder is installed place the blank in the B position and the samples in positions 1 to 5 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 4 Press Measure Sample 46 GENESYS 10S UV Vis User Guide Thermo Scientific 11 Abs and T Measurements Advanced A T C Taking Measurements The sample measurement appears If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually Thermo Scientific GENESYS 10S UV Vis User Guide 47 11 Abs and T Measurements Advanced A T C Taking Measurements 48 GENESYS 10S UV Vis User Guide Thermo Scientific ee ARE Basic Concentration Measurements Basic A T C This chapter covers Basic Concentration Measurements Us
58. g Scan Data 3 Enter the correct value and then press Min Y Max Y Min X or Max X to accept it The instrument redraws the plot using the entered minimum and maximum values Performing Calculations on the Scan Data You can modify your graph by performing calculations on the data In the Edit Graph screen press Math The Math screen provides these capabilities Labeling Peaks and Valleys Smoothing Data e Calculating the Area Under a Curve Determining Peak Height Using a 3 point Net Equation Scanning 9 15 15Feb02 Test Name FILTER BLANK 2 220 Wave length 500 0 IDH 1 Baseline collected 15Feb02 SRR nee e IUE Off 1 Net Ualleus Labeling Peaks and Valleys To label valleys and peaks 1 With your scan displayed on the edit graph screen press Math 62 GENESYS 10S UV Vis User Guide Thermo Scientific 14 Scanning Viewing and Manipulating Scan Data Scanning 13 40 20Jun0Z Test Name Filter BLANK 0 730 Label Peaks Label Valleys Features Press f or to select Press ENTER to change Press ESC to exit Wave length IDit 1 Baseline collected 20Jun02 2 Press Peaks amp Valleys to display the Label Peaks and Valleys window 3 Select the type of labels to display and press Enter The instrument labels the selected items on your scan data plot Note Up to nine peaks or valleys can be calculated and displayed Smoothing Data If your scan shows sampling noise you can smooth the data w
59. g the Test 4c Succ vi aderire d oe asec Sec gnaec ney Mel oca 108 Internal Printer Test ow oe tet voee y qu ettet eraot dota cet i RR en 108 Maltife alite cese eee Re ROS UR A pa a o eR CD e 111 Ro tine Cate sisted ehh oS OA A Rc te e ERRORS 111 Cleaning and Maintaining Cells idest ters e 112 Cleaning the Windows of the Sample Compartment 114 Changing the Fuse isses s a s OL atardecer a Rain 114 Parameters MI 117 Calculations for Software lsesseeeeeeeeeeeeeeeeeeee 127 Calculations for Oligo Calculator cece cece eee eee ee ees 131 Thermo Scientific LL m Preface Congratulations on your purchase of a Thermo Scientific spectrophotometer Our spectrophotometers integrate advanced hardware features with the power and flexibility of a wide range of accessories Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following Note Notes contain helpful supplementary information IMPORTANT Follow instructions labeled Important to avoid damaging the system hardware or losing data CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation which if not avoided could result in death or serious injury Thermo Scientific GE
60. h absorbance readings and 2 contaminants in the cell may react chemically with subsequent reagents or standards introduced into the cell Cleaning methods depend to some extent on the nature of the contaminating material It is important to identify the residual material in the cell that needs to be removed Refer to the following table for suggestions on cleaning methods solvents and material Solvents Aqueous Examples Protein Biologics DNA Suggested Cleaning Methods Warm water with detergent Dilute nitric acid lt 10 rinse Copious water rinse Aqueous Salt solutions Dilute nitric acid lt 10 rinse Copious water rinse Aqueous Basic solutions Warm water with detergent Dilute nitric acid lt 10 rinse Copious water rinse Organic Hydrocarbons small molecules oils Rinse with organic solvent Warm water with detergent Dilute nitric acid lt 10 rinse Copious water rinse Organic 112 GENESYS 10S UV Vis User Guide Alcohol solutions Rinse with similar alcohol acetone or other solvent Copious water rinse Thermo Scientific Thermo Scientific 22 Maintenance Routine Care Solvents Examples Suggested Cleaning Methods Organic Acidic solutions Rinse with organic solvent e Warm water with detergent Dilute nitric acid lt 10 rinse Copious water rinse Organic Hydrocarbons small molecules oils Rinse with organic solvent e Warm water with deterge
61. h the value range and then type the complete entry and press Enter Menu Selection With the parameter Units or Sample Positioner highlighted press Enter to display the selection list Highlight the appropriate item and press Enter Advanced A T C 17 05 28Feb0 Test Name Measurement Mo Wave length Ref Wavelengt Ref Wavelengt Delay Time mi Conc of Standa Factor nits Sample Positio Number of Samp Unit More parameter Press f or to select Press ENTER Press Tor 1t L mmi 00 Unit On Off Toggle With the parameter e g AutoPrint highlighted press Enter to toggle to the opposite value Alphanumeric Entry With the parameter e g Test Name highlighted press Enter The Name Entry screen appears Highlight the desired character and press Add Character When you are finished press Accept Name 8 GENESYS 10S UV Vis User Guide Thermo Scientific 2 Setting Up the Instrument Setting Utility Parameters Create Test Name 17 25 28Feb0 ABCDEFGH I JKLMNOPQRS TUUUXVZ0123456789 abcdefghi jklmnopqrstuvuxuzB p Ctt Test Name Copper Test 1 Press f or to select character Press ficcept Name to save the test Name Character Character Name Setting Utility Parameters The Utility menu lets you set certain non test hardware parameters such as the date and time standby setting screen contrast settings and printer setup You can also access a directory of all stored tests and the calculator funct
62. hat hexane is in the blank position and toluene in hexane is in cell 1 pO Uerification 16 37 2Mar0 esolution Maximum Minimum Ratio Result 0 100 0 037 2 672 Pass Place Hexane in the Blank position Place Toluene in Hexane in Cell 1 ress Start Test 2 Press Start Test The results indicate pass or fail for each wavelength If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Contact technical support for more troubleshooting advice Photometric Accuracy This test measures the absorbance or T of a set of standards and compares the results with specified tolerances The wavelength absorbencies and tolerances are preset but you should change them to the values on the calibration certificate included with your standards Note You can display the tolerances for this test in either absorbance or Transmittance When running the Photometric Accuracy test remember 104 GENESYS 10S UV Vis User Guide Thermo Scientific Selecting the Mode 21 Performance Verification Photometric Accuracy You need Photometric Accuracy standards calibrated to known absorbance values at specified wavelengths Measure the standards in the order they are listed on the test screen You can use one to five standards To display the Photometric Accuracy screen Highlight Photometric Accuracy Press Enter erformance Uerification 16 46 2Mar0 hotometric Ac
63. ific GENESYS 10S UV Vis User Guide iii Contents Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 iv GENESYS 10S UV Vis User Guide Installing the Internal Printer Optional ues arnt deo dtes 21 Loading Paper in the Internal Printer cece ao ensem ee ee lees 22 External Printet us bec e ur e are etl ebbe ep ERE ME 23 Sample Positioner Setting 0 0c eee cece e eee eee eee eens 25 AEQUO Lo ara ea S ae as Ae eee But Res Bisse Aare a ache RU A T nS SS 25 AUTO Cm 25 Single Cell Holder s oe satis bu acus Loa es eet bri bet Wicca dus Mat we 25 Manual Grae Sat srk e copeM ete xax tps od aue Lotes ced het p 26 Cell COLTBCUOT coru Sora docta a OU acit a RE RALIS EE VLA E ask 27 Cell Correction Tsao sc ast nE ye whorl acest PP Ve ane pae o epa 27 Specifying Wavelengths for Discrete nms Mode lusus 29 Managing Stored Tests 0 cece cece eee eee eee teeeeneee 31 Software PAsswoldo gi aw ce Cote ex het ka cq teclas ed 31 amiga Test ao pcs petii ap a a o pal ba fpa Op Ea T poat acce 31 Saving a Testo sino e RE 32 Eoading Fest Files ood ed Mer A eg Re ERE NOE e LP qaid 34 Lock Unlock uda ERE DOE ERE anneal aie en Ser an pto ict 35 Deleting a Test RC aie eara aay E ea i enan e 35 SmartStart ooe Eion Ennn Tiy EDD en d UR ACA ENEE GOD CO RAI d M oy 37 Concentration Units tition ane iene va vidus AE Edo ER 4 ERE ICD Ra 39 Specifying Concentration UUfrts
64. ing Conc Std to Measure Concentration Using Conc Factor to Measure Concentration Measuring Samples Basic Concentration Measurements Use Basic ATC mode to make basic concentration measurements This basic concentration mode is useful for very simple comparisons that do not require a standard curve You cannot save data in this application or factors used when a single standard is measured For better accuracy and the ability to save and recall methods and data use the Standard Curve application mode described in Concentration Measurements Standard Curve Application on page 83 Measuring concentration using the Basic ATC mode is similar to measuring Absorbance or 96 T Basic ATC mode lets you measure concentration using either a factor or one standard to convert absorbance readings to concentration units When using a factor specify the factor and concentration units When using a standard specify the concentration of the standard and measure its absorbance When Basic ATC is set to Conc Std or Conc Factor you can perform these tasks Setting the Wavelength and Mode Measuring a Blank Measuring a standard or entering a factor Measuring Samples Thermo Scientific GENESYS 10S UV Vis User Guide 49 12 Basic Concentration Measurements Basic A T C Setting the Wavelength and Mode The steps for taking measurements in the two modes are similar the only difference is whether you measure a standard or enter a fac
65. ion You cannot set utility parameters or change the utility when the instrument is carrying out a measurement Press Utility on the keypad Utility 4 09pm 56ug09 Softuare Revision of lamp life used Screen Contrast ress T or to select parameter Setting the Date and Time Highlight Date Time Setup and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 9 2 Setting Up the Instrument Setting Utility Parameters Utility 3 39pm 16Mar0 Time Format Set Time 3 39pm ress T or to select item to change You can modify the date time format and time To set the date 1 Highlight Set Date and press Enter 2 Press Set Date type the date and press Enter 3 Press Set Month highlight the correct month and press Enter 4 Press Set Year type the year and then Enter 5 Press Esc to save the settings To select the time format You can set the instrument to display the time in either am pm or 24 hour format To change the format highlight Time Format and press Enter until the desired format AM PM or 24 hour appears Tosetthe time 1 Highlight Set Time and press Enter 2 To set the hour press Set Hour type in the hour and press Enter 3 To set the minutes press Set Minute type in the minute and press Enter 4 To select between AM and PM if in AM PM time format press Set AM PM until the appropriate setting appears Note Your changes are saved au
66. ith the smoothing function To smooth data With your scan data displayed on the edit graph screen press Smoothing On Determining Peak Height Using a 3 point Net Equation To determine 3 point net measurements 1 With your scan data displayed on the edit graph screen press Math 2 Press 3 Pt Net The 3 point net measurement screen shows the cursor options and three cursor lines designated for the left center and right wavelengths Thermo Scientific GENESYS 10S UV Vis User Guide 63 14 Scanning Viewing and Manipulating Scan Data Scanning 13 45 20Jun02 Test Name Filter BLANK 407 415 425 0 045 0 384 0 041 0 400 A 0 020 400 Wavelength 435 3 Pt Net Value 0 341 Factor 1 000 nter Nex Factor Cursor Cursor Use Cursor and Cursor lt to position the left cursor line to the desired wavelength value The instrument calculates the 3 point net absorbance for the selected wavelengths Continue selecting the other wavelengths by pressing Next Cursor to activate the center and right cursor lines Select the wavelengths with Cursor and Cursor Repeat until all three wavelengths have been selected Press Enter Factor to access the set factor box Enter the desired factor and press Enter The instrument calculates the value for the 3 point net absorbance for the selected wavelengths multiplied by the selected factor Calculating the Area Under a Curve t v 1 2 To calcu
67. ive thumbscrew s 4 Close the sample compartment door Note If the cell holder is not aligned correctly you will not be able to tighten the thumbscrews Removing the 6 Position Cell Holder and the Single Cell Holder To remove the 6 Position Cell Holder and the Single Cell Holder 1 Open the sample compartment door and let it rest on its hinge 2 With one hand loosen the captive thumbscrew 3 With your other hand pull straight up on the cell holder and lift it out of the sample compartment 4 Close the sample compartment door Installing Accessory Cell Holders Make sure that you have the correct cell holder baseplate installed 20 GENESYS 10S UV Vis User Guide Thermo Scientific 3 Accessories Installing the Internal Printer Optional Note To use 100 mm long pathlength cells you must install the Single Cell Holder baseplate See Cell Holder Configurations on page 15 for the different cell holder accessories that can be created for your instrument Each of the Cell Holders needs to be installed on either a single cell baseplate or a multi cell baseplate by removing the cell holder s secured to the baseplate Each cell holder has a captive screw in the bottom of the holder that secures the holder to the baseplate Use a flat blade screwdriver to loosen the captive screw from the baseplate and lift the cell holder from the baseplate Then insert the new cell holder into the appropriate position and se
68. late the area under a curve With your scan data displayed on the edit graph screen press Math Press Area to display the Area Under the Curve measurement screen 64 GENESYS 10S UV Vis User Guide Thermo Scientific 14 Scanning Viewing and Manipulating Scan Data Scanning 13 50 20Jun0Z Test Name Filter BLANK 440 460 0 075 0 060 0 730 A 0 000 425 Wave length 500 fireaCTangent 4 933 Factor 1 000 e Nex Options Cursor Cursor 3 Use Cursor and Cursor lt to position the left cursor line to the desired wavelength value The instrument calculates the area under the curve for the selected wavelengths 4 Continue selecting the other wavelengths by pressing Next Cursor to activate the next cursor line Select the wavelength with Cursor and Cursor Press Set Options to access the set options window Highlight Factor Enter the desired factor and press Enter N ON WwW Highlight Calculation baseline 8 Press Enter to toggle between Zero and Tangent 9 Press Esc to return to the area under a curve screen The instrument calculates the area under a curve for the selected wavelengths factor and calculation method Viewing and Rescaling Tabular Scan Data When working with tabular scan data you must press Edit Data before performing other functions on the data Thermo Scientific GENESYS 10S UV Vis User Guide 65 14 Scanning Viewing and Manipulating Scan Data Scanning 10 05 15Feb02 Test
69. ll holder is installed the message Error Single Cell Holder found Use Single Cell Holder appears Press Accept Change to continue the measurement with a single cell or install the 6 cell changer and press Cancel Change See the parts list for a more detailed list of available accessories Selecting and Positioning Cuvettes The compatible wavelength range for different types of cells depends on the material used Cell Type Wavelength Optical Glass 360 nm to gt 1100 nm Borosilicate Glass 330 nm to gt 1100 nm Disposable Quartz 190 nm to gt 1100 nm Polystyrene gt 340 nm Methacrylate gt 300 nm Acrylic gt 280 nm UV transparent gt 220 nm Note See the manufacturer s specifications and work within the recommended range Thermo Scientific GENESYS 10S UV Vis User Guide 5 1 Spectrophotometer Basics Z dimensions Note The pathlength of test tubes is not as well defined as that of square cuvettes Other Guidelines Position cuvettes and test tubes so that the clear sides face the light beam one clear side facing the front of the instrument and the other facing the back Note Always place test tubes in the instrument in exactly the same orientation in the light beam An alignment mark on the test tube helps you orient the test tubes consistently and correctly When using small aperture small volume cells Always used cells with black masking Use the same cell or cuvette for y
70. mmediate measurement If you select more than one test as SmartStart tests the instrument when powered on automatically displays a menu containing only those tests Note You can always access the default main menu by pressing Test To set up a single test SmartStart 1 Press Utility to display the Utility screen Utility 11 04 28Feb0 Softuare Revision 0 014b Instrument Serial Number rinter Off Date Time Setup 11 04 28Feb0 beeper of lamp life used Screen Contrast ress T or to select parameter 2 Highlight Stored Tests Directory and press Enter 3 Highlight the appropriate test and press Select Test An arrow sign gt indicates the test has been selected for the SmartStart menu Press Esc to return to the Utility screen or power down the instrument Thermo Scientific GENESYS 10S UV Vis User Guide 37 7 SmartStart To unselect a test 1 Press Utility 2 Highlight Stored Tests Directory and press Enter 3 Highlight the test to be removed and press Unselect Test The Main Menu will be displayed upon power up To set up a multiple test SmartStart 1 Follow steps 1 through 3 in the procedure above for setting up a single test SmartStart 2 Select the desired tests An arrow sign gt indicates the tests selected for the SmartStart menu Press Esc to return to the Utility screen or power down the instrument Note To remove tests from the SmartStart menu see the pro
71. moves to the B position to measure the blank and returns to its previous position With the list of wavelengths and factors displayed press Measure Samples to measure and display the absorbance at each wavelength If you set the measurement mode to Concentration Factor the calculated concentration at each wavelength also appears If a 6 Position Cell Holder is installed press Cell Position to reposition the cell holder and measure the rest of the samples manually 70 GENESYS 10S UV Vis User Guide Thermo Scientific o ME Absorbance Ratio The Absorbance Ratio application lets you measure the absorption ratio of two different wavelengths Reference wavelength correction is available to eliminate the effects of a sample matrix Typically used in quality control applications an absorbance ratio provides a convenient and quick diagnostic test for sample quality Use Absorbance Ratio for Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples To get started press Test highlight Absorbance Ratio and press Enter Recalling a Test fibsorbance Ratio 10 32 20Feb02 BLANK Test Name 456 Saved 1 Wavelength 1 260 Onm Wavelength 2 280 Onm Ref Wavelength Correction Off Sample Positioner Auto 6 Number of Samples 5 More parameters Press f or to select item to change EE Dum I E Test Tests Test To recall atest 1 In the Absorbance Ratio screen press Stored Te
72. ng a Test To recall a test Press Stored Tests 2 Highlight the test and press Enter or Load Test to display the parameters for the selected test Thermo Scientific GENESYS 10S UV Vis User Guide 53 13 Concentration Measurements Advanced A T C Setting Up Test Parameters Setting Up Test Parameters t v l 2 To set up test parameters Highlight the desired parameter Some parameters appear only if you select a concentration mode while others appear regardless of the selected measurement mode See Parameters for a complete list When the parameters are set press Save Test to save the test or Run Standard to measure a standard if in Conc Std or press Run Test if in Conc Factor Measuring a Standard To measure a standard automatically using Auto 6 or Auto 3 With Measurement Mode set to Conc Std press Run Standard Advanced A xT C 10 17 20Feb02 Test Name STANDARD TEST BLANK Conc Abs Factor ppb 650 0nm calculated S Entry Enter a number 0 001 to 9999 Enter the concentration of the standard and press Enter Press Measure Blank Insert the blank and standard Press Enter to measure the blank and samples and display the absorbance and calculated factor To measure a standard manually using Manual 6 or Single Cell Holder Press Run Standard Enter the concentration of the standard and press Enter Insert the blank and standard 54 GENESYS 10S UV Vis User Gui
73. nt Dilute nitric acid lt 10 rinse Copious water rinse IMPORTANT Keeping the cell clean is very important for long cell life Never store cuvettes long term in a water or solvent bath between uses If the solvent you are using dries impurities in the water or solvent may be deposited on the inside of the cell causing permanent damage Useonly lens cleaning tissue paper or fine soft cloth to wipe optical surfaces Most paper products such as facial tissues paper towels etc contain wood fibers that can damage the cell material At the end of the day ensure that all cells are well cleaned and stored in a suitable container after drying Term Definition Dilute acid Dilute nitric acid lt 10 Acid Hydrochloric 5M acid or nitric acid 5M see the Note below Solvent rinse Rinse with the solvent that was originally used to solvate your analyte Copious water rinse Use a pure water e g deionized distilled RO and rinse at least 10 times Detergent Use a neutral pH detergent Triton X 100 if available to dilute acid wash water rinse to remove residue Note Do not use 5M nitric acid on an Anti reflection mirror coated cell IMPORTANT Using an ultrasonic cleaning bath for your cells is not recommended Each bath generates a different frequency therefore if your bath operates at the resonant frequency of a cell the cell will break If a cell was cleaned in an ultrasonic bath th
74. o change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history MN E Contents Lj PT vii Safety and Special Notices amp dae cip aor ERROR RE hoe es E DEOR EROR vii Chapter1 Spectrophotometer Basics suuseseeeeeeeeeeeennenen 1 Spectroph tometer Components o4 echa ecd avec ora cw denade snows rra 1 CONNECTS aa sch phat pe ac ERG ER haa wad eda tea reped eg E quies 1 Abaeutthe Revpad sires lores E CERRO ORC oC Cd aoi E C 3 SINIT DTE 4 6 Position Cell Holders iiis azar EE ERE RE RUE Ac bacio aeo Edo a 5 Singlet el EO Pg nei rine veh CER EL Eae paloe Vaga 5 Selecting and Positioning Cuvettes aac exe seb cea ies RARE ARCET 5 Ful c 4 sie
75. oes not include U MW 312 2 x A 303 2 x T 329 2 x G 289 2 x C 18 02 If entry does include U MW 329 2 x A 306 2 x U 345 2 x G 305 2 x C 18 02 Percentage Molecular weight x Da M Absorptivity units A units T If entry does not include U Extinction 260 units G units C E0 15 200 x A 8 400 x T coefficient units U 12 010 x G 7 050 x C M lcm If entry does include U E0 15 200 x A 9 900 x U 12 010 x G 7 050 x Conversion Factor N A Molecular Weight x 10 pg mL Extinction Coefficient Calculation of Tp units A units T Ty2 2 A T G C C Oligos up to 20 bases units G units C in length Thermo Scientific GENESYS 10S UV Vis User Guide 131 25 Calculations for Oligo Calculator Calculation Entry Parameters Formula Displayed Units Calculation of Tp units A units T T 81 5C 16 6 log Na C DNA DNA hybrids units G units C 1 0 7 Na 0 51 GC f 500 L P 0 63 formamide e M molarity of cation Fraction GC fraction of G and C form formamide in the sample L of base pairs P mismatching Calculation of Tp units A units T T 67 C 16 6 log Na C DNA RNA hybrids units G units C 1 0 7 Na 0 8 GC 500 L P 0 5 96 formamide M molarity of cation Fraction GC fraction of G and C 96 f
76. on buttons do not function when you select Single Cell Holder Note You can cause the 6 Cell Changer to function as a single cell holder and measure only one cell by selecting this option However we recommend that the single cell holder be used for accessories requiring a high degree of positioning repeatability such as the nanoCell accessory small aperture microcells the coupling module of fiber optics probes and flowcells used with a sipper system Thermo Scientific GENESYS 10S UV Vis User Guide 25 4 Sample Positioner Setting Manual 6 Manual 6 Measure a blank and up to five samples without changing cuvettes in the cell changer using the cell position buttons to advance the cell changer to the appropriate position for the next measurement Place the blank in the blank position and your samples in the other cell positions Regardless of where the cell holder is positioned when you press Measure Blank the cell holder automatically goes to the blank position and measures the blank However you can use the cell position buttons to select a different position for the measurement Note When you have the Cell Changer installed the instrument always considers the material in the B position as a blank This means that even after measuring your blank the first time you can place samples only in positions 1 through 5 26 GENESYS 10S UV Vis User Guide Thermo Scientific LESE Cell Correction Every test setup screen provides
77. or with the shiny metal contacts faces away from the printer and towards the plastic door Use the clip on the hinge to secure the wires Install the printer door by sliding it back onto the hinge Close the lamp door Tighten the captive screw on the printer door to hold it securely in place Loading Paper in the Internal Printer Paper roll holder Paper entry slot Finger tab Icon for paper direction 22 GENESYS 10S UV Vis User Guide Thermo Scientific 3 Accessories External Printers Note Make sure the paper roll holders are in place as shown When installed correctly they fit flush with the top of the instrument To load paper in the internal printer 1 Cut the paper so the edge is even Note Arrows on the paper roll holders indicate the direction of the paper feed 2 Feed the paper straight into the paper entry slot The printer grabs the end of the paper and pulls it in 3 In Basic ATC mode when the paper stops press Enter to continue advancing the paper until the paper comes out of the paper exit slot 4 Pull out on the finger tabs on the paper roll holders and secure the roll of paper onto the paper roll holder External Printers Your spectrophotometer is able to print to external desktop printers supporting HP PCL 5 0 format and later Note PCL format does not support HP Windows printers To print to an HP PCL printer connect the USB cable to the printer and to the U
78. orm 96 formamide in the sample L of base pairs P mismatching Calculation of Tp units A units T T 78 C 16 6 log Na C RNA RNA hybrids units G units C 1 0 7 Na 0 7 GC f 500 L P 0 35 formamide e M molarity of cation Fraction GC fraction of G and C 96 form 96 formamide in the sample L of base pairs P 96 mismatching Conversion from ug MI and molecular weight pmol uL L ug mL x 1000 DNA Mol Wt from Oligo calc factor test pmol p 132 GENESYS 10S UV Vis User Guide Thermo Scientific
79. orrection application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Measuring Samples Note If a 6 Postion Cell Changer is installed place the blank in position B and the sample in cell position 1 The instrument always uses cell position 1 to scan the sample To measure samples 1 In the Kinetics screen press Run Test 2 If a 6 Position Cell Holder is installed place the blank in position B and the sample in position 1 3 Press Measure Sample Thermo Scientific GENESYS 10S UV Vis User Guide 93 20 Kinetics Recalling and Recalculating Graphical Kinetics Results After the measurement the kinetics data and rate appear Ifa Single Cell Holder is installed press Measure Blank to measure the blank insert your sample and then press Measure Sample After the measurement the kinetics data and rate appear Kinetics 3 05pm 27Jan01 Test Name ENZYME Cell mm 0 740 A 0 583 Time 3 00 IDit A min Result 1 0 050 22 69 Tabular Graph Sample To switch between tabular and graphical displays press Graph Tabular On a graphical display you can press Edit Graph and then press Cursor to move the cursor line from one position to another on the plot As the cursor moves the rate and result values indicate the values for the point where the cursor is located Recalling and Recalcul
80. our blank and your samples Z dimensions The figure below illustrates the position of the light beam in the instrument Beam size specifications are shown below Distance from bottom of cuvette to center of beam Z dimension 8 5 mm Beam dimensions 2 mm wide by 7 mm tall 8 5 mm 6 GENESYS 10S UV Vis User Guide Thermo Scientific 0 IAM Setting Up the Instrument Setting up the instrument involves Entering Parameter Values Setting Utility Parameters Standby Mode Setting Up the Internal Printer Entering Parameter Values The following sections describe the use of the keypad to interact with menus and controls These sections provide instructions for Numeric Entry Menu Selection e On Off Toggle e Alphanumeric Entry Numeric Entry With the parameter e g Wavelength highlighted start typing the numeric value An Entry window with the value range appears Type the complete entry and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 7 2 Setting Up the Instrument Entering Parameter Values Advanced A x T C 17 02 28Feb0 BLANK Test Name Copper Measurement Mode Conc Std ave length 546nm Ref Wavelength Correction On Ref Wavelength 320nm Delay Time min sec 0 00 Conc of Standard Undef ined Factor Undef ined Units C Sample Positioner futo 6 Number of Samples 1 Entry 7 Enter Wavelength 190 1100 Alternatively you can press Enter to display the Entry window wit
81. rbance Transmittance and Concentration press Change Mode You can toggle modes whenever you see Change Mode Absorbance 16 03 31Jan02 BLANK O 4 6A 660 Onm Cae To TURN Blank Mode When Basic ATC is set to Absorbance or Transmittance these capabilities are provided Setting the Wavelength Measuring a Blank Measuring Samples Setting the Wavelength To set the wavelength 1 Press Set nm or any number key to set the wavelength Thermo Scientific GENESYS 10S UV Vis User Guide 43 10 Abs and T Measurements Basic A T C Measuring a Blank Absorbance 17 25 i 0 219A Ef inm Enter Wavelength 190 1100 2 Enter the wavelength for taking measurements and press Set nm again Measuring a Blank To measure a blank 1 Place the blank in the cell holder If a 6 Position Cell Changer is installed place the blank in the B position 2 To enter an absorbance or transmittance value for the blank press a number key and enter the desired value in the Entry field 3 Press Measure Blank Measuring Samples If a 6 Position Cell Changer is installed place the samples in the cell positions and press the corresponding cell position button to move the cell holder to the measuring position The absorbance ABS or percent transmittance T measurement appears on the display If a Single Cell Holder is installed remove the blank and place the sample in the cell holder The absorbance or Transmittan
82. rements Advanced A T C Measuring Samples 56 GENESYS 10S UV Vis User Guide Thermo Scientific S A Scanning The wavelength scanning application lets you measure the absorption or percent transmission spectrum of a sample You can use scans to determine peak wavelengths or to evaluate the quality of a material Use the Scanning application for e Recalling a Test Setting Up Test Parameters Cell Correction Collecting a Baseline Scan Scanning a Sample Viewing and Manipulating Scan Data Rescaling Graphical Scan Data Determining Peak Height Using a 3 point Net Equation e Calculating the Area Under a Curve Labeling Peaks and Valleys Note The Scanning application lets you measure only one sample at a time Auto 6 Auto 3 and Manual 6 are not available for scanned measurements Note To set a baseline expiration time press Utility and then highlight Baseline Expiration Press Enter and set the desired time To get started press Test highlight Scanning and press Enter Recalling a Test To recall a test Press Stored Tests 2 Highlight the test you want to recall and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 57 14 Scanning Setting Up Test Parameters The parameters for the selected test appear This screen provides these capabilities Setting Up Test Parameters Setting up Cell Correction Scanning Start Wavelength Stop Wavelength Sample Positioner Sc
83. return to an identical wavelength in a repeatable manner The test uses the internal xenon lamp A xenon lamp has a strong fundamental line 529 nm This line is an essential property of xenon and serves as a fundamental standard When running the internal standard test remember that The wavelength and tolerance values are preset and cannot be changed The cell holder should be empty Torun the Wavelength Repeatability test 1 Highlight Wavelength Repeatability and press Enter 2 Press Start Test The results indicate pass or fail for each wavelength Derfocaance Uerification 16 33 2Mar0 avelength Repeatabilitu inimum Maximum Peak to Peak Result 29 2 529 3 0 1 Pass ake sure the Blank position is empty ress Start Test If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Contact technical support for more troubleshooting advice Thermo Scientific GENESYS 10S UV Vis User Guide 103 21 Performance Verification Resolution Resolution This test measures the ability of the spectrophotometer to resolve adjacent features in a spectrum The test is performed using a 0 02 v v solution of toluene in hexane and requires a hexane blank When running the internal standard test remember that the wavelengths and tolerance values are preset and cannot be changed To run the Resolution test 1 Highlight Resolution and press Enter Ensure t
84. return to the screen displaying the factor and units 6 Press Select Units 7 Highlight the appropriate unit in the list and press Enter 8 Press Esc to return to the Conc With Factor screen Measuring Samples If the 6 Position Cell Changer is installed place the sample you want to measure in one of the cell positions and press the corresponding cell position button to move the cell changer to the measuring position The measurement appears If the Single Cell Changer is installed remove the blank and place the sample in the cell changer The measurement appears 52 GENESYS 10S UV Vis User Guide Thermo Scientific ee m Concentration Measurements Advanced A T C Use the Advanced A T C application for concentration measurements for Selecting a measurement mode concentration with one standard or concentration with a factor e Recalling a Test Setting Up Test Parameters Measuring a Standard or Entering a Factor only if you select either concentration with one standard or concentration with a factor Measuring a Blank Measuring Samples To get started press Test highlight Advanced A T C and press Enter Advanced amp T C 15 40 26Jun02 Test Name AAA Measurement Mode Conc Std Wave length 546nm Ref Wavelength Correction Off Delay Time min sec 0 00 Conc of Standard 100 0 Factor 1587 Units C More parameters Press f or to select item to change Standard Test Tests Test Recalli
85. rinter test Press Start Test You can press Stop Test to stop the test The test print routine appears on the printed results If the test fails follow these guidelines Thermo Scientific Make sure the internal printer is the selected as the printer device on the Utility screen Make sure the internal printer is installed correctly Return to the main screen and press Enter If the paper does not move the printer may not be installed correctly Make sure the thermal paper is threaded with the thermal side toward the printer head the outside surface of the roll is the thermal surface For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us GENESYS 10S UV Vis User Guide 109 21 Performance Verification Internal Printer Test 110 GENESYS 10S UV Vis User Guide Thermo Scientific Maintenance The spectrophotometer is durable and reliable so routine maintenance is minimal This section explains Routine Care Changing the Fuse ra WARNING Operating the instrument with the cover off exposes the operator to potentially dangerous voltages and ultraviolet UV radiation Therefore we recommend that only authorized service representatives perform procedures requiring removal of the instrument cover and replacement of electrical components To protect both yourself and the instrument be sure to contact
86. rument recalculates and displays the new data plot Press Edit Scale to modify the scale In the edit scale screen you can Use Auto Scale to change the scale and display the new graph Use Manual Scale to change the scale and display the new graph Use the cursor to identify specific points along the X axis 60 GENESYS 10S UV Vis User Guide Thermo Scientific itN v 14 Scanning Viewing and Manipulating Scan Data To use the Auto Scale function With your scan data displayed on the edit scale screen press Auto Scale The instrument adjusts the minimum and maximum values for the X and Y axes so all the data appears on the plot t v To use the Cursor 1 With your scan data displayed on the edit scale screen press Cursor Scanning 13 36 20Jun02 Test Name Filter BLANK 0 730 Wave length 500 Wave length Abs 415 Use Cursor to move to the desired minimum wavelength value Press Set Min X to redraw the plot using the new minimum wavelength value Repeat using Cursor and Set Max X to set the new maximum wavelength To use the Manual Scale function Press Manual Scale to display the manual scale options Scanning 9 01 15Feb02 Test Name FILTER BLANK 2 220 Wave length 500 0 IDE 1 Baseline collected 15Feb02 2 To set the minimum or maximum value for the X or Y axis press Min Y Max Y Min X or Max X GENESYS 10S UV Vis User Guide 61 14 Scanning Viewing and Manipulatin
87. s Performs a specific function as displayed above each key Functions will change depending on the software screen Some function keys may not be active Clears the value being entered Returns to the previous screen Deletes the last character entered GENESYS 10S UV Vis User Guide 3 1 Spectrophotometer Basics Cell Holders Key or button Function Accepts highlighted entered or selected values Advances to the next parameter or screen Prints the method or results to the selected printer If PC is selected for the printer sends the method or results to the USB port Displays a menu of software applications Displays the Utility screen Controls the location of the cursor Highlights the value or option for the selection ecce 6 OO O 0000 Enters numbers a decimal point and a minus sign for values Cell Holders 4 Cell position keys Selects the cell holder position to be measured B blank and 1 5 sample positions A 9 2 positions when used in Auto 3 mode Your instrument includes a 6 position and single cell holder GENESYS 10S UV Vis User Guide Thermo Scientific 1 Spectrophotometer Basics Selecting and Positioning Cuvettes 6 Position Cell Holder Note If the Cell Positioner method option is set to Auto 6 whenever you press Run Test to start a measurement the instrument attempts to initialize the cell positioner If a single ce
88. s the procedure to collect the data necessary to correct for absorbance differences between cuvettes all test types GENESYS 10S UV Vis User Guide 123 23 Parameters 124 Parameter Description Slope Enters AAbs AConcentration value Standard Curve test type Smoothing Turns data smoothing on and off Scanning Software Revision Displays the version of firmware in the instrument Utility SRE tolerance Standard Concentrations Acceptable minimum stray light Enters the concentration of standards used to generate the standard curve for the test Standby Start wavelength Selects the time since the last keystroke or instrument activity powers down the unit to save lamp life Utility Enters the beginning wavelength for a scan Scanning tests only Statistics Turns stats on or off if ON calculates the average and Std Dev of results Statistics registers are cleared when Statistics OFF and or when instrument is OFF and or when test parameters are changed and or when test is saved or resaved in all test types except Kinetics Scanning Multiwavelength Std Concentration Enters the concentration of the analyte in the standard solution Stop wavelength Enters the ending wavelength for a scan Scanning tests only Stored Tests Directory Displays the list of tests stored in the instrument Utility Tabular Displays the list of collected data Kinetics
89. samples 3 Press Enter To measure samples manually using Manual 6 or Single Cell Holder 1 In the Absorbance Difference screen press Run Test 2 Install the blank and samples A 6 Position Cell Holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position fibsorbance Difference 13 39 14Feb02 Test Name TEST 9 Cell 1 Abs Abs Abs Result IDH 429 0 630 0 Ref WL ppm ie TI Blank GENESYS 10S UV Vis User Guide 71 17 Absorbance Difference Measuring Samples 4 Press Measure Sample If a 6 Position Cell Holder is installed press the position buttons to reposition the cell holder and measure the rest of the samples manually 78 GENESYS 10S UV Vis User Guide Thermo Scientific 0 BE 3 Point Net The 3 Point Net application lets you determine the height of a peak based on a sloping baseline drawn between two wavelengths on either side of the peak This type of analysis is beneficial when the precise peak height is needed for a particular assay A factor can be multiplied by the measured peak height to give the concentration of the measured analyte in the appropriate concentration units Use 3 Point Net for Recalling a Test Setting Up Test Parameters Cell Correction Taking Measurements To get started press Test highlight 3 Point Net and press Enter 3 Point Net 10 11 15Feb02 BLA
90. select the Thermo Scientific Thermo Scientific 2 Setting Up the Instrument Setting Up the Internal Printer To set the utility parameters for the printer Press Utility Highlight Printer and press Enter Utility 4 11pm 5Aug09 Software Revision 4 000d Instrument Serial Number 2RO1234567 Internal n External off Press f or to select Press ENTER to change Press ESC to exit ress T or to select parameter 3 Select the printer and press Enter until On appears 4 Press Esc to save the settings Note Text and graphics can be output via the internal printer and an external printer on the USB port Only text no graphics can be output via the USB connection to a computer GENESYS 10S UV Vis User Guide 13 2 Setting Up the Instrument Setting Up the Internal Printer 14 GENESYS 10S UV Vis User Guide Thermo Scientific MEN Accessories This chapter briefly describes the sampling and system accessories that are available for your spectrophotometer Complete descriptions and operating instructions are included with the accessories You can install or remove these accessories without switching off the instrument Cell Holders and Cell Holder Accessories The instrument is shipped with both the 6 Position Cell Holder installed at the factory and the Single Cell Holder Directions to remove and install these cell holders and other cell holder accessories are below Cell Holder
91. seline Starts the collection of the baseline Scanning only Concentration Sets the concentration value Conc of Standard Displays the entered concentration value Adv A T C Correction Mode Selects the mode for cell correction Discrete nms or Scan Cursor Goes to Cursor tracking mode to view data points in the graph Kinetics Scanning Cursor Moves the cursor right or left on the graph and displays the lt Cursor data of each point Kinetics Scanning Curve Fit Selects the type of line fit calculation Standard Curve tests Data File Name Allows entry of a name for the data file when AutoSave ON Date Cell Correction Date Standards Measured GENESYS 10S UV Vis User Guide Displays the date when cell correction data on cuvettes was last collected Displays the date when standards were last measured with this instrument Standard Curve tests Thermo Scientific Parameter Date Time Setup 23 Parameters Description Enters the current date and time settings for the instrument Utility Delay Time Enters the time from Test Initiation to first measurement allows for sample equilibration ADV A T C and Kinetics Delete Character Deletes the last character of Name entry Test Name and Edit Units Delete File Deletes a test or data file from the Stored Tests Directory Utility Delete Name Deletes the entire name to allow a new entry Test Name and Edit
92. sor setting Scan Area and Scan 3 Pt Net calculations in the graph Scanning Number of bases Enters the number of bases in the oligonucleotide Oligos tests Number of Matched Enters the number of cuvettes that will be run in the Cuvettes Correction Program maximum of 5 Number of Samples Enters the number of samples to be measured in the test Not available in Kinetics or Scanning Number of Standards Enters the number of standards to be measured for the standard curve Printer Selects the output mode internal RS 232 Parallel Utility Printout Contrast Lets you improve visibility of internal printer hardcopy by changing the darkness of the print Utility Protein Factor Enters the factor to calculate Protein concentration DNA Bio Tests Ref Wavelength Enters a reference wavelength value for each reported measurement measures the analytical wavelength and reference wavelength Reported measurement Abs Analytical WL Abs Reference WL Ref Wavelength Correction 122 GENESYS 10S UV Vis User Guide Turns reference wavelength correction on or off Thermo Scientific Thermo Scientific 23 Parameters Parameter Description Run Corr Initiates the collection of cuvette data for cell correction Run Standard Goes to the Standards entry screen Run Test Goes to the data collection screen all tests Sample Positioner Selects the type of Positioner 1 Cell no movem
93. ss Stored Tests 2 Highlight the test to recall and press Enter Kinetics ef Wavelength Correction Delay Time min sec isplay Result Rate Factor More parameters ress T or to select item to change L liz Du ne Test Tests Test This screen provides these capabilities Setting Up Test Parameters Setting up Cell Correction Saving a Test Viewing the list of stored tests Measuring a Blank Measuring Samples Setting Up Test Parameters To set up test parameters 1 In the Kinetics screen highlight the desired parameter 92 GENESYS 10S UV Vis User Guide Interval Time min sec 0 01 Total Run Time Chr min sec 0 05 00 easure Blank Once Thermo Scientific 20 Kinetics Measuring Samples Kinetics ef Wavelength Correction Delay Time min sec 0 00 Interval Time min sec 0 01 Total Run Time hr min sec 0 05 00 easure Blank Once isplay Result Rate Factor On More parameters Parameter Description Delay Time Enters the time from Test Initiation to first measurement allows for sample equilibration Adv Interval Time Enters the time between repeated readings Measure Blank Selects the frequency of zeroing the instrument as function key Once or Every Reading 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or sample Note If Cell Correction is ON you must run the Setup C
94. st 1 Lese Test With the Blank position empty insert the 2A filter in position 1 Ignore the test results at 2A if you do not have a filter installed in position 1 Press Start Test The results of the test indicate pass or fail for each wavelength If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Make sure the instrument is warmed up for at least 30 minutes with the standby mode feature turned off For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us GENESYS 10S UV Vis User Guide 107 21 Performance Verification Stray Light Stray Light This test measures the stray light at selected wavelengths and compares the measurements with expected values The wavelengths and expected values are preset and cannot be changed Running the stray light test takes about 30 seconds When running the stray light test remember Running the Test You need Stray Light standards designed to measure stray light at 220 nm 340 nm and 400 nm i e must have lt 0 1 T at the wavelength of interest Position B should be empty Use position 1 for the 220 nm stray light standard SRE 220 or equivalent Use position 2 for the 340 nm stray light standard SRE 340 or equivalent Use position 3 for the 400 nm stray light standard SRE 400 or equivalen
95. sts Thermo Scientific GENESYS 10S UV Vis User Guide 71 16 Absorbance Ratio Setting Up Test Parameters 2 Highlight the test to recall and press Enter This screen provides these capabilities Setting Up Test Parameters Cell Correction Saving a Test Viewing the list of stored tests Measuring a Blank Setting Up Test Parameters To set up test parameters 1 In the Absorbance Ratio screen highlight the desired parameter 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Samples Measuring Samples Absorbance Ratio 13 14 14Feb02 Test Name BLANK Abs Abs Abs ID 660 0 640 0 Ref WL Result Insert samples 1 5 into positions 1 5 Press ENTER to measure L1 1 1 Jj To measure samples automatically using Auto 6 or Auto 3 1 Install the blank and samples 72 GENESYS 10S UV Vis User Guide Thermo Scientific Thermo Scientific 16 Absorbance Ratio Measuring Samples Press Enter To measure samples manually using Manual 6 or Single Cell Holder In the Absorbance Ratio screen press Run Test Install the blank and sample A 6 Position Cell Holder can hold five samples
96. t In the Stray Light screen make sure the wavelengths and tolerances are set correctly To run the Stray Light test Highlight Stray Light Press Enter Press Start Test The results indicate pass or fail for each wavelength If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Make sure all the filters being used are specifically designed to measure stray light at the specific wavelengths Verify that the filters are placed in the correct cell positions For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us Internal Printer Test This test lets you verify that the internal printer is functional To run the test you will need to have an internal printer installed Running the internal printer test takes no more than 20 seconds after you press Stop 108 GENESYS 10S UV Vis User Guide Thermo Scientific KD l 21 Performance Verification Internal Printer Test To run the Internal Printer test From the Utility screen verify that the internal printer is installed properly and is selected If necessary press Utility and then select the internal printer In the Performance Verification screen highlight Internal Printer Test Press Enter Performance Verification 16 57 2Mar0 Internal Printer Test Press Start Test to start p
97. tall the cell changer or press Accept Change to continue with using the single cell holder To prevent the Fatal Error whenever removing the cell changer always return to either the main menu or the test parameter menu of the test before removing the cell changer Changing Cell Holders To use long pathlength cells cylindrical or rectangular 18 GENESYS 10S UV Vis User Guide Thermo Scientific 3 Accessories Cell Holders and Cell Holder Accessories use test tubes measure solid samples in the filter holder regulate sample temperature via an external liquid recirculator you must install the appropriate cell holders The 6 Position Cell Holder installed in the spectrophotometer can easily be removed to install other accessory cell holders See Removing the 6 Position Cell Holder and the Single Cell Holder on page 20 Installing the 6 Position Cell Holder and the Single Cell Holder To install the 6 Position Cell Holder and the Single Cell Holder 1 Open the sample compartment door and let it rest on its hinge 2 With one hand carefully lower the cell holder straight down into the sample compartment Figure 1 6 Position Cell Holder Captive thumbscrew Thermo Scientific GENESYS 10S UV Vis User Guide 19 3 Accessories Cell Holders and Cell Holder Accessories Figure 2 Single Cell Holder Captive thumbscrews Single cell holder Alignment pin hole 2 3 With your other hand tighten the capt
98. the 3 Point Net Setup screen displayed and the parameters set press Run Test to display the 3 Point Net measurement screen 80 GENESYS 10S UV Vis User Guide Thermo Scientific 18 3 Point Net Taking Measurements 3 Point Net 10 25 15Feb02 Test Name BILIRUBIN BLANK Abs Abs Abs Result ID 429 0 515 0 630 0 C Insert sample 1 into position 1 Press ENTER to measure L1 1 S 2 Install che blank and samples 3 Press Enter To take measurements manually using Manual 6 or Single Cell Holder 1 Press Run Test 3 Point Net 9 26 4Feb02 Test Name 3 PTNET Cell 1 Abs Abs Abs Result ID 630 0 660 0 680 0 pg mL Measure Bink RN 2 Install the blank and samples If a 6 Position Cell Holder is installed place the blank in the B position The holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its cell position 4 Press Measure Sample If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually Thermo Scientific GENESYS 10S UV Vis User Guide 81 18 3 Point Net Taking Measurements 82 GENESYS 10S UV Vis User Guide Thermo Scientific 3m99 Concentration Measurements Standard Curve Application The Standard Curve application lets you perform a quantitati
99. tomatically even during power down by battery backup 10 GENESYS 10S UV Vis User Guide Thermo Scientific 2 Setting Up the Instrument Setting Utility Parameters Standby Mode To prolong lamp life your spectrophotometer has been pre set at the factory to automatically go into standby mode after 15 minutes of inactivity Setting Baseline Expiration Time If you will be performing scans on your samples you can set a time limit for which a collected baseline will be valid This is particularly useful when measurements are made in a production setting across multiple shifts or when the nature of the blank sample changes dramatically with time To set the baseline expiration time 1 Highlight Baseline Expiration hr min and press Enter Utility 3 47am 16Mar07 Software Revision 1 200 Instrument Serial Number 2M7A123005 Printer Internal de i Setup 3 47am 16Mar0 of lamp life used Screen Contrast rintout Contrast Stored Tests Directory Entru Enter time 00 10 24 00 O FF 2 Enter the desired time in the Entry baseline expiration time field and press Enter Setting the Screen Contrast To make it easier to read the display you can adjust the screen contrast on the instrument To set the screen contrast 1 Highlight Screen Contrast and press Enter Thermo Scientific GENESYS 10S UV Vis User Guide 11 2 Setting Up the Instrument Setting Up the Internal Printer 2 Adjust the contrast by follo
100. tor Setting the Wavelength and Mode To set the wavelength and mode 1 Press Set nm or any other number key to set the wavelength 2 Enter the wavelength for taking measurements and press Set nm again 3 Press Change Mode until the appropriate measurement mode Concentration with Standard or Concentration with Factor appears Using Conc Std to Measure Concentration In this mode a standard sample of well known concentration is used to determine the concentration of samples The concentration of unknown samples is determined ratiometrically from the measured standard The measurement can be expressed mathematically as Standard Concentration Sample Concentration Abs T of Standard Abs 96T of Unknown Sample where the standard concentration is precisely known the Abs T of the standard and the Abs T of the unknown sample are measured and the sample concentration is calculated To use Conc Std to measure concentration 1 If necessary press Change Mode to switch to the Concentration with Standard mode Conc Std 16 15 31Jan02 Factor 1 Std 1 C BLANK 0 478C 660 Onm Bree Ps al Blank Standard Mode If a 6 Position Cell Changer is installed place the blank in the B position and the standard in position 1 50 GENESYS 10S UV Vis User Guide Thermo Scientific 12 Basic Concentration Measurements Basic A T C Using Conc Factor to Measure Concentration 2 Press Measure Blank If th
101. tum to a result Abs WL2 x Factor Result Abs Ratio Abs Diff Multiwavelength tests Factor 3 31 Enters a factor to convert a datum to a result Abs WL3 31 x Factor Result Multiwavelength tests Graph Displays the graph of collected data Kinetics Scanning ID Enters the numeric identifier for measurement autoincrements during the test until turned off set to 0 Instrument Serial Number Displays the serial number of the instrument Utility Intercept Enters where the line crosses the Y axis Abs where conc 0 Interval Enters the wavelength range between data points Scanning tests only Interval Time 120 GENESYS 10S UV Vis User Guide Enters the time between repeated readings Kinetics only Thermo Scientific Thermo Scientific 23 Parameters Parameter Description Linearity Value Enters a linearity value Kinetics only To help determine the linearity of the reaction during the measurement the instrument offers a linearity parameter This is the difference between the changes in the absorbance of two measurements as shown in the following example Time Abs AA Linearity 1 1 a 2 wD al 3 29 09 P 4 38 09 P 5 46 08 P 6 52 06 F Linearity is the AA between AA calculations P Pass and F Fail Load Test Loads the highlighted test from the Stored Tests Directory into active memory and sets the instrument to the test parameters Utility
102. ve analysis experiment using a multipoint calibration curve A calibration curve is composed of standards of well known concentration A fit of this standard curve is used to measure the concentration of samples Use Standard Curve for Creating a standard curve set up the parameters and then measure the standards for the curve Cell Correction Measuring Samples Viewing calibration curve data select between graphical and tabular displays Editing a Standard Curve change the number of standards select a different curve fit or delete points from the curve Viewing and saving sample data measured using the standard curve To get started press Test highlight Standard Curve and press Enter Standard Curve 15 28 4Feb02 Test Name AMMONIA Date Standards Measured 4 Wave length 660 Onm Ref Wavelength Correction Off Curve Fit Linear Number of Standards Number of Samples More parameters Press f or to select item to change saa Eu umm Standards Test Tests Thermo Scientific GENESYS 10S UV Vis User Guide 83 19 Concentration Measurements Standard Curve Application Recalling a Standard Curve Recalling a Standard Curve To recall a standard curve 1 Press Stored Tests 2 Highlight the test to recall and press Enter Setting the Parameters for a Standard Curve To set the parameters for a standard curve 1 Place the standards in their cell positions 2 Set parameters for measuring th
103. wing the instructions on the screen Utility 3 48am 16Mar07 Software Revision 1 200 Instrument Serial Number 2M7A123005 rinter Internal ate Time Setup 3 48am 16Mar07 tandby 15 min aseline Expiration Chr min Off Press fT for darker contrast Press for lighter contrast Press ESC to exit 3 Press Esc Setting Up the Internal Printer To set up the internal printer you need to set its internal parameters and load the paper t v 1 To set up the internal printer Install the internal printer If you ordered the internal printer as a separate item you need to install it See Installing the Internal Printer Optional on page 21 section in Accessories for instructions Load paper in the printer See Loading Paper in the Internal Printer on page 22 in Accessories for instructions on installing the printer Setting the Utility Parameters for the Printer Paper printouts are available from both the internal printer and a USB printer attached to the instrument Alternatively displayed ASCII text and graphics can be sent to a computer using a USB connection Enabling the PC as the printing device sends ASCII data to the PC via the USB connection to the PC No graphics are sent A program on the PC is required to capture and use the data not provided appropriate device 12 GENESYS 10S UV Vis User Guide To ensure that the instrument can output information correctly to the printer

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