RNA clean-up
Contents
1. Entwickelt bei Ber hrung mit S ure giftige Gase Precaution phrases P 210 Keep away from heat sparks open flames hot surfaces No smoking Von Hitze Funken offener Flamme hei en Oberfl chen fernhalten P 233 Keep container tightly closed Beh lter dicht verschlossen halten P 260 Do not breathe vapours Dampf nicht einatmen P 273 Avoid release to the environment Freisetzung in die Umwelt vermeiden P 301 312 IF SWALLOWED Call a POISON CENTER or doctor physician if you feel unwell BEI VERSCHLUCKEN Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt anrufen P 330 Rinse mouth Mund aussp len Bei Symptomen der Atemwege GIFTINFORMATIONSZENTRUM oder Arzt anrufen P 403 235 Store in a well ventilated place Keep cool K hl an einem gut bel fteten Ort augbewahren For further information please see Material Safety Data Sheets www mn net com Weiterf hrende Informationen finden Sie in den Sicherheitsdatenbl ttern www mn net com MACHEREY NAGEL 03 2012 Rev 03 11 NucleoSpin RNA Clean up 5 5 1 Protocols RNA Clean up Before starting the preparation Check if Wash Buffer RA3 was prepared according to section 3 Sample preparation Fill up RNA Fill up RNA samples smaller than 100 uL with RNase free sample to water to 100 uL 100 pL with RNA samples from 100 200 uL should be filled up with water RNase free water to 200 uL 2 Preparation of lysis binding buffer prem2. 03 9 RNA clean up 4 Safety instructions risk and safety phrases The following components of the NucleoSpin RNA Clean up kits contain hazardous contents Wear gloves and goggles and follow the safety instructions given in this section 4 1 Risk and safety phrases Component Hazard contents Hazard Risk Safety symbol phrases phrases Inhalt Gefahrstoff Gefahrstoff R S tze S S tze symbol RA1 Guanidinium thiocyanate 30 60 x Xn R 20 21 22 S 13 61 Guanidiniumthiocyanat 30 60 32 52 53 RA2 Guanidinium thiocyanate 30 60 x Xn R 10 S 13 16 61 ethanol 20 35 20 21 22 Guanidiniumthiocyanat 30 60 32 52 53 Ethanol 20 35 Risk phrases R10 Flammable Entz ndlich R 20 21 22 Harmful by inhalation in contact with skin and if swallowed Gesundheitssch dlich beim Einatmen Verschlucken und Ber hrung mit der Haut R 32 Contact with acids liberates very toxic gas Entwickelt bei Ber hrung mit S ure sehr giftige Gase R 52 53 Harmful to aquatic organisms may cause long term adverse effects in the aquatic environment Sch dlich f r Wasserorganismen kann in Gew ssern l ngerfristig sch dliche Wirkungen haben Safety phrases S13 Keep away from food drink and animal foodstuffs Von Nahrungsmitteln Getr nken und Futtermitteln fernhalten S16 Keep away from sources of ignition No smoking Von Z ndquellen fernhalten Nicht rauchen S61 Avoid release to the environment Refer to special instr
3. 2 Reagents consumables and equipment to be supplied by user Reagents 96 100 ethanol to prepare Wash Buffer RA3 and to adjust RNA binding conditions Consumables 1 5 mL microcentrifuge tubes Sterile RNase free tips Equipment Manual pipettors Centrifuge for microcentrifuge tubes Personal protection equipment e g lab coat gloves goggles 1 3 About this user manual It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin RNA Clean up kit is used for the first time Experienced users however may refer to the Protocol at a glance instead The Protocol at a glance is designed to be used only as a supplemental tool for quick referencing while performing the purification procedure All technical literature is available on the internet at www mn net com Please contact Technical Service regarding information about changes of the current user manual compared to previous revisions MACHEREY NAGEL 03 2012 Rev 03 5 RNA clean up 2 Product description 2 1 The basic principle One of the most important aspects in the isolation and handling of RNA is to prevent degradation of the RNA during the isolation procedure With the NucleoSpin RNA Clean up kit RNA containing samples are mixed with a solution containing large amounts of chaotropic ions This solution immediately inactivates RNases which are present in virtually all biological materials and creates appro
4. damages or defects arising in shipping and handling transport insurance for customers excluded or out of accident or improper or abnormal use of this product defects in products or 20 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up components not manufactured by MACHEREY NAGEL or damages resulting from such non MACHEREY NAGEL components or products MACHEREY NAGEL makes no other warranty of any kind whatsoever and SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF ANY KIND OR NATURE WHATSOEVER DIRECTLY OR INDIRECTLY EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION AS TO THE SUITABILITY REPRODUCTIVITY DURABILITY FITNESS FOR A PARTICULAR PURPOSE OR USE MERCHANTABILITY CONDITION OR ANY OTHER MATTER WITH RESPECT TO MACHEREY NAGEL PRODUCTS In no event shall MACHEREY NAGEL be liable for claims for any other damages whether direct indirect incidental compensatory foreseeable consequential or special including but not limited to loss of use revenue or profit whether based upon warranty contract tort including negligence or strict liability arising in connection with the sale or the failure of MACHEREY NAGEL products to perform in accordance with the stated specifications This warranty is exclusive and MACHEREY NAGEL makes no other warranty expressed or implied The warranty provided herein and the data specifications and descriptions of this MACHEREY NAGEL product appearing in MACHEREY NAGEL published
5. with phenol based protocols depends very much on the performed procedure RNA in biological samples is not protected against digestion until the sample material is flash frozen or disrupted in the presence of RNase inhibiting or denaturing agents Therefore it is important that biological samples are flash frozen in liquid N immediately and stored at 70 C or processed as soon as possible Samples can be stored in lysis buffer after disruption at 70 C for up to one year at 4 C for up to 24 hours or up to several hours at room temperature Frozen samples are stable up to 6 months Frozen samples in lysis buffer should be thawed slowly before starting with the isolation of total RNA Wear gloves at all times during the preparation Change gloves frequently MACHEREY NAGEL 03 2012 Rev 03 7 RNA clean up 2 4 Elution procedures It is possible to adjust the elution method and the volume of RNase free water used for the subsequent application of interest In addition to the standard method described in the individual protocols recovery rate about 70 90 there are several modifications possible High yield Perform two elution steps with the volume indicated in the individual protocol About 90 100 of bound nucleic acid will be eluted High yield and high concentration Elute with the standard elution volume and apply the eluate once more onto the column for reelution Eluted RNA should immediately be placed and always k
6. 740963 1 set Visit www mn net com for more detailed product information MACHEREY NAGEL 03 2012 Rev 03 19 RNA clean up 6 3 Product use restriction warranty NucleoSpin RNA Clean up kit components are intended developed designed and sold FOR RESEARCH PURPOSES ONLY except however any other function of the product being expressly described in original MACHEREY NAGEL product leaflets MACHEREY NAGEL products are intended for GENERAL LABORATORY USE ONLY MACHEREY NAGEL products are suited for QUALIFIED PERSONNEL ONLY MACHEREY NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING For detailed information please refer to the respective Material Safety Data Sheet of the product MACHEREY NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT MACHEREY NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application Application on the human body is STRICTLY FORBIDDEN The respective user is liable for any and all damages resulting from such application DNA RNA PROTEIN purification products of MACHEREY NAGEL are suitable for IN VITRO USES ONLY ONLY MACHEREY NAGEL products specially labeled as IVD are also suitable for IN VITRO diagnostic use Please pay attention to the package of the product IN V TRO diagnostic products are expressly marked as IVD on the packaging IF THERE IS NO IVD SIGN THE PRODUCT SHALL
7. NOT BE SUITABLE FOR IN VITRO DIAGNOSTIC USE ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE INCLUDING BUT NOT LIMITED TO DIAGNOSTIC THERAPEUTIC AND OR PROGNOSTIC USE No claim or representations is intended for its use to identify any specific organism or for clinical use included but not limited to diagnostic prognostic therapeutic or blood banking It is rather in the responsibility of the user or in any case of resale of the products in the responsibility of the reseller to inspect and assure the use of the DNA RNA protein purification products of MACHEREY NAGEL for a well defined and specific application MACHEREY NAGEL shall only be responsible for the product specifications and the performance range of MN products according to the specifications of in house quality control product documentation and marketing material This MACHEREY NAGEL product is shipped with documentation stating specifications and other technical information MACHEREY NAGEL warrants to meet the stated specifications MACHEREY NAGEL s sole obligation and the customer s sole remedy is limited to replacement of products free of charge in the event products fail to perform as warranted Supplementary reference is made to the general business terms and conditions of MACHEREY NAGEL which are printed on the price list Please contact us if you wish to get an extra copy There is no warranty for and MACHEREY NAGEL is not liable for
8. RNA clean up User manual NucleoSpin RNA Clean up March 2012 Rev 03 MACHEREY NAGEL MN RNA clean up Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 5 1 3 About this user manual 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Handling preparation and storage of starting materials T 2 4 Elution procedures 8 3 Storage conditions and preparation of working solutions 9 4 Safety instructions risk and safety phrases 10 4 1 Risk and safety phrases 10 4 2 GHS classification 11 5 Protocols 12 5 1 RNA Clean up 12 5 2 RNA isolation from up to 10 cells 14 6 Appendix 16 6 1 Troubleshooting 16 6 2 Ordering information 18 6 3 Product use restriction warranty 20 MACHEREY NAGEL 03 2012 Rev 03 3 RNA clean up 1 Components 1 1 Kit contents NucleoSpin RNA Clean up 10 preps 50 preps 250 preps REF 740948 10 740948 50 740948 250 Lysis Buffer RA1 10 mL 25 mL 125 mL Wash Buffer RA2 15 mL 15 mL 80 mL Wash Buffer RA3 5 mL 12 5 mL 3 x 25 mL Concentrate RNase free H O 5mL 15 mL 65 mL NucleoSpin RNA Binding 10 50 250 Columns light blue rings plus Collection Tubes Collection Tubes 2 mL 10 50 250 Collection Tubes 1 5 mL 10 50 250 User manual 1 1 1 For preparation of working solutions and storage conditions see section 3 4 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up 1
9. Tube Add 350 uL Buffer RA3 to the NucleoSpin RNA Binding Column Centrifuge for 2 min at 8 000 x g Transfer the NucleoSpin RNA Binding Column to a nuclease free Collection Tube 1 5 mL supplied Open the lid of the column and let the membrane dry for 3 min If for any reason the liquid level in the Collection Tube has reached the NucleoSpin RNA Binding Column after centrifugation discard flow through and centrifuge again The procedure ensures complete removal of ethanol from the column Elute RNA Elute the RNA in 60 pL RNase free H O supplied and centrifuge at 8 000 x g for 1 min If higher RNA concentrations are desired elution can be done with 40 uL Overall yield however will decrease when using smaller volumes For further alternative elution procedures see section 2 4 Load 700 uL lysate 8 000 x g 30s 700 pL RA3 8 000 x g 30s 350 pL RA3 8 000 x g 2 min 60 pL RNase free H O 8 000 x g 1 min MACHEREY NAGEL 03 2012 Rev 03 13 NucleoSpin RNA Clean up 5 2 RNA isolation from up to 10 cells Before starting the preparation Check if Wash Buffer RA3 was prepared according to section 3 Sample preparation As sample material use up to 10 cells in a volume of up to 100 uL Cell Iysis Add 300 uL Buffer RA1 and vortex vigorously in order to lyse the cells Adjust RNA binding conditions Add 300 uL ethanol 96 100 to t
10. catalogues and product literature are MACHEREY NAGEL s sole representations concerning the product and warranty No other statements or representations written or oral by MACHEREY NAGEL s employees agent or representatives except written statements signed by a duly authorized officer of MACHEREY NAGEL are authorized they should not be relied upon by the customer and are not a part of the contract of sale or of this warranty Product claims are subject to change Therefore please contact our Technical Service Team for the most up to date information on MACHEREY NAGEL products You may also contact your local distributor for general scientific information Applications mentioned in MACHEREY NAGEL literature are provided for informational purposes only MACHEREY NAGEL does not warrant that all applications have been tested in MACHEREY NAGEL laboratories using MACHEREY NAGEL products MACHEREY NAGEL does not warrant the correctness of any of those applications Last updated 07 2010 Rev 03 Please contact MACHEREY NAGEL GmbH amp Co KG Tel 49 24 21 969 270 tech bio mn net com MACHEREY NAGEL 03 2012 Rev 03 21
11. d Binding of RNA to the silica membrane is only effective in the presence of ethanol Kit storage Store kit components at room temperature Storage at low temperatures may cause salt precipitation Keep bottles tightly closed in order to prevent evaporation or contamination Sample material Sample material not stored properly Whenever possible use fresh material If this is not possible flash freeze the samples in liquid N Samples should always be kept at 70 C Never allow tissues to thaw before addition of lysis buffer Perform disruption of samples in liquid No Contamination The NucleoSpin RNA Clean up procedure does not comprise a DNA digestion step Therefore the extent of DNA contamination mainly depends on the sample material ot NN a A If lowest level of DNA contamination is desired use one of genomie the rDNase containing NucleoSpin RNA kits see ordering information 16 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up Problem Possible cause and suggestions Suboptimal performance of RNA in downstream experiments Carry over of ethanol or salt Do not let the flow through touch the column outlet after the second wash using Wash Buffer RA3 Be sure to centrifuge at the corresponding speed for the respective time in order to remove ethanolic Wash Buffer RA3 completely Check if Wash Buffer RA3 has been equilibrated to room temperature before use Washing at lower temperatures low
12. e incorporation reverse transcriptase PCR RT PCR and for DNA RNA based chip hybridisations e g MWG rat microarray MWG Ebersberg Germany or Human Genome U133A Array Affymetrix USA Integrity of purified RNA originally isolated from for example eukaryotic cells is examined by denaturing agarose gel electrophoresis rRNA bands are sharp with the 28S band being about twice as intense as the 18S band The standard protocol section 5 1 allows the clean up of up to 200 ug of RNA per NucleoSpin RNA Binding Column or the isolation of total RNA from up to 1 x 10 cultured cells section 5 2 6 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up Table 1 Kit specifications at a glance Parameter NucleoSpin RNA Clean up Technology Silica membrane technology Format Mini spin columns Sample material lt 100 uL RNA sample with single column loading containing up to 200 ug RNA lt 200 uL RNA sample with double column loading containing up to 200 ug RNA Up to 10 cells Fragment size gt 200 nt Typical recovery 85 95 0 1 200 ug RNA input Aago Aaso 1 9 2 1 Elution volume 40 120 uL Preparation time Approx 20 min 6 preps Binding capacity 200 ug 2 3 Handling preparation and storage of starting materials RNA intended to be used as sample for the NucleoSpin RNA Clean up procedure should be handled with the same care as any RNA sample The stability of prepurified RNA samples e g RNA isolated
13. ept on ice for optimal stability because almost omnipresent RNases general lab ware fingerprints dust will degrade RNA For short term storage freeze at 20 C for long term storage freeze at 70 C 8 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up 3 Storage conditions and preparation of working solutions Attention Buffers RA1 and RA2 contain chaotropic salt Wear gloves and goggles CAUTION Buffers RA1 and RA2 contain guanidinium thiocyanate which can form highly reactive compounds when combined with bleach sodium hypochlorite DO NOT add bleach or acidic solutions directly to the sample preparation waste All kit components should be stored at room temperature 18 25 C and are stable up to one year Storage at lower temperatures may cause precipitation of salts Check that 96 100 ethanol is available as additional solution in the lab Before starting any NucleoSpin RNA Clean up protocol prepare the following Wash Buffer RA3 Add the indicated volume of 96 100 ethanol see table below to Wash Buffer RA3 Concentrate Mark the label of the bottle to indicate that ethanol was added Store Wash Buffer RA3 at room temperature 18 25 C for up to one year NucleoSpin RNA Clean up 10 preps 50 preps 250 preps REF 740948 10 740948 50 740948 250 Wash Buffer RA3 5 mL 12 5 mL 3x25 mL Concentrate Add 20 mL ethanol Add 50 mL ethanol Add 100 mL ethanol to each bottle MACHEREY NAGEL 03 2012 Rev
14. ers efficiency of salt removal by Wash Buffer RAS A 2 min centrifugation with a subsequent 3 min drying with open lid is sufficent for an extensive removal of ethanol from the column Residual ethanol will typically be around 1 Increasing the drying step with open lid from 3 min to 20 min will decrease the residual ethanol content commonly to below 0 1 but also RNA recovery will be reduced 5 20 Store isolated RNA properly Eluted RNA should always be kept on ice for optimal stability since trace contaminations of omnipresent RNases general lab ware fingerprints dust will degrade the isolated RNA For short term storage freeze at 20 C for long term storage freeze at 70 C RNA concentration is too low For highest RNA concentration and most sensitive downstream applications NucleoSpin RNA Clean up XS is recommended NucleoSpin RNA Clean up XS allows elution in only 5 20 uL volume see ordering information Higher RNA yield than theoretically possible If performing clean up of samples containing less than approximately 300 ng RNA subsequent quantification by Aago measurement may simulate yields larger than the RNA input This may be due to absorbance of silica abrasion In order to prevent incorrect A eo quantification of small RNA amounts centrifuge the elution tube for 30 s at 8 000 11 000 x g and withdraw an aliquot for measurement without disturbing any sediment or use a silica abrasion insensitive RNA
15. he lysate and mix by vortexing or pipetting up and down After addition of ethanol a stringy precipitate may become visible which will not affect the RNA isolation Be sure to mix thoroughly an apply sample as homogeneous solution onto the column Bind RNA For each preparation take one NucleoSpin RNA Binding Column light blue placed in a Collection Tube and load the lysate 700 uL Centrifuge for 30s at 8 000x g Discard Collection Tube with flow through and place the column in a new Collection Tube Maximal loading capacity of NucleoSpin RNA Binding Columns is 750 uL Repeat the procedure if larger volumes are to be processed Fill up sample to 100 pL e g with PBS 300 pL RA1 Vortex 300 uL ethanol 96 100 Mix 8 000 x g 30s H Load lysate 14 MACHEREY NAGEL 03 2012 Rev 03 NucleoSpin RNA Clean up Wash and dry silica membrane Add 250 uL Buffer RA2 to the NucleoSpin RNA Binding Column Centrifuge for 30 s at 8 000 x g Discard flow through and reuse Collection Tube Add 700 pL Buffer RA3 to the NucleoSpin RNA Binding Column Centrifuge for 30 s at 8 000 x g Discard flow through and reuse Collection Tube Add 350 uL Buffer RA3 to the NucleoSpin RNA Binding Column Centrifuge for 2 min at 8 000 x g Transfer the NucleoSpin RNA Binding Column to a nuclease free Collection Tube 1 5 mL supplied Open the lid of the column and let the membrane dry for 3 m
16. in If for any reason the liquid level in the Collection Tube has reached the NucleoSpin RNA Binding Column after centrifugation discard flow through and centrifuge again The procedure ensures complete removal of ethanol from the column Elute RNA Elute the RNA in 60 uL RNase free H O supplied and immediately centrifuge at 8 000 x g for 1 min If higher RNA concentrations are desired elution can be done with 40 uL Overall yield however will decrease when using smaller volumes For further alternative elution procedures see section 2 4 250 uL RA2 8 000 x 9 30s 700 pL RA3 8 000 x g F 30 s 350 pL RA3 8 000 x g 2 min 60 uL RNase free H O 8 000 x g 1 min MACHEREY NAGEL 03 2012 Rev 03 15 RNA Clean up 6 Appendix 6 1 Troubleshooting Problem Possible cause and suggestions RNA is degraded no RNA obtained Poor RNA quality or yield RNase contamination Create an RNase free working environment Wear gloves during all steps of the procedure Change gloves frequently Use of sterile disposable polypropylene tubes is recommended Keep tubes closed whenever possible during the preparation Glassware should be oven baked for at least 2 hours at 250 C before use Reagents not applied or restored properly Sample and reagents have not been mixed completely Always vortex vigorously after each reagent has been added No ethanol has been adde
17. ix y g p Prepare Prepare a Buffer RA1 ethanol premix with a ratio of 1 1 premix For each 100 pL RNA sample mix 300 pL Buffer RA1 Mix and 300 uL of ethanol 96 100 300 pL RA1 with If multiple samples are processed the preparation of a 300 uL ethanol master premix is recommended e g 2 mL Buffer RA1 96 100 2 mL 98 ethanol for approximately 6 preparations 3 Adjust RNA binding conditions To 100 uL RNA sample add 600 uL 6 volumes of Buffer RA1 ethanol premix Mix sample with premix by vortexing 6 vol premix If a 200 uL RNA sample is processed add 1200 uL Buffer RA 1 ethanol premix Mix After addition of ethanol a stringy precipitate may become visible which will not affect the RNA isolation Be sure to mix thouroughly and apply sample as homogeneous solution onto the column 12 MACHEREY NAGEL 03 2012 Rev 03 NucleoSpin RNA Clean up Bind RNA For each preparation take one NucleoSpin RNA Binding Column light blue ring placed in a Collection Tube and load the lysate 700 uL Centrifuge for 30s at 8 000x g Discard Collection Tube with flow through and place the column in a new Collection Tube Maximal loading capacity of NucleoSpin RNA Binding Columns is 750 uL Repeat the procedure if larger volumes are to be processed Wash and dry silica membrane Add 700 pL Buffer RA3 to the NucleoSpin RNA Binding Column Centrifuge for 30 s at 8 000 x g Discard flow through and reuse Collection
18. priate binding conditions which favor adsorption of RNA to the silica membrane Simple washing steps remove salts metabolites organics like phenol and macromolecular cellular components Pure RNA is finally eluted under low ionic strength conditions with RNase free water supplied The RNA clean up preparation using NucleoSpin RNA Clean up kits can be performed at room temperature The eluate however should be treated with care because RNA is very sensitive to trace contaminations of RNases often found on general lab ware fingerprints and dust To ensure RNA stability keep RNA frozen at 20 C for short term or 70 C for long term storage 2 2 Kit specifications NucleoSpin RNA Clean up kits are ideal for the clean up of total RNA from RNA preparations which contain inacceptable amounts of RT PCR inhibitors e g RNA prepared with phenol chloroform based methods The kit is further recommended for the isolation of RNA from small amounts of cultured cells whenever copurification of some genomic DNA is acceptabel The kits allow purification of pure RNA with an Aggo Azgy ratio generally exceeding 1 9 measured in TE buffer pH 7 5 NucleoSpin RNA Clean up kits are recommended for the clean up of RNA from enzymatic reactions like in vitro transcribed RNA amplification reactions biotinylated RNA or fluorescent Cy dye labeled RNA The purified RNA is ready to use for applications like enzymatic labelling reactions e g dy
19. quantification method e g RiboGreen fluorescent dye MACHEREY NAGEL 03 2012 Rev 03 17 RNA Clean up 6 2 Ordering information Product REF Pack of NucleoSpin RNA Clean up 740948 10 10 preps 740948 50 50 preps 740948 250 250 preps NucleoSpin RNA Clean up XS 740903 10 10 preps 740903 50 50 preps 740903 250 250 preps NucleoSpin RNA Il 740955 20 20 preps 740955 50 50 preps 740955 250 250 preps NucleoSpin RNA XS 740902 10 10 preps 740902 50 50 preps 740902 250 250 preps NucleoSpin RNA L 740962 20 20 preps NucleoSpin RNA Blood 740200 10 10 preps 740200 50 50 preps NucleoSpin miRNA 740971 10 10 preps 740971 50 50 preps 740971 250 250 preps NucleoSpin RNA Plant 740949 20 20 preps 740949 50 50 preps 740949 250 250 preps NucleoSpin FFPE RNA 740969 10 10 preps 740969 50 50 preps 740969 250 250 preps NucleoSpin 8 RNA 740698 12x 8 preps 740698 5 60 x 8 preps NucleoSpin 96 RNA 740709 2 2 x 96 preps 740709 4 4x 96 preps 740709 24 24 x 96 preps NucleoMag 96 RNA 744350 1 1x96 preps 744350 4 4x 96 preps DISTRIBUTION AND USE OF NUCLEOSPIN TRIPREP IN THE USA IS PROHIBITED FOR PATENT REASONS 18 MACHEREY NAGEL 03 2012 Rev 03 RNA Clean up Product REF Pack of NucleoSpin TriPrep 740966 10 10 preps 740966 50 50 preps 740966 250 250 preps NucleoSpin RNA Protein 740933 10 10 preps 740933 50 50 preps 740933 250 250 preps Buffer RA1 740961 50 mL 740961 500 500 mL rDNase Set
20. uctions safety data sheet Freisetzung in die Umwelt vermeiden Besondere Anweisungen einholen Sicherheitsdaten bl tter zu Rate ziehen Hazard labeling not neccessary if quantity per bottle below 125g or ml certificate of exemption according to 67 548 EEC Art 25 1999 45 EC Art 12 and German GefStoffV 20 3 and TRGS 200 7 1 For further information see Material Safety Data Sheet 10 MACHEREY NAGEL 03 2012 Rev 03 RNA clean up 4 2 GHS classification Only harmful features do not need to be labeled with H and P phrases until 125 mL or 125 g Mindergef hrliche Eigenschaften m ssen bis 125 mL oder 125 g nicht mit H und P S tzen gekennzeichnet werden Component Hazard contents GHS symbol Hazard Precaution phrases phrases Inhalt Gefahrstoff GHS Symbol H S tze P S tze RA1 Guanidinium thiocyanate Warning 302 412 260 273 30 60 EUH031 301 312 330 Guanidiniumthiocyanat Achtung 30 60 RA2 Guanidinium thiocyanate Warning 226 302 210 233 260 30 60 ethanol 20 412 273 301 312 35 EUH031 330 403 235 Guanidiniumthiocyanat Achtung 30 60 Ethanol 20 35 Hazard phrases H 226 Flammable liquid and vapour Fl ssigkeit und Dampf entz ndbar H 302 Harmful if swallowed Gesundheitssch dlich bei Verschlucken H 412 Harmful to aquatic life with long lasting effects Sch dlich f r Wasserorganismen mit langfristiger Wirkung EUH031 Contact with acids liberates toxic gas
Download Pdf Manuals
Related Search