AUTOCLAVE - Codice n
Contents
1. SAFETY INSTRUCTIONS e Use the device just for the indicated purposes e The Device must be correctly used according to this instructions manual before starting any operation e Replace immediately any electrical cable when damaged never use a damaged or worn electrical cable e Always disconnect the charger before A Repairing or maintenance these operations must be carried out by qualified staff B Keep the unit clean e Use original spare parts and accessories for any replacement e Do not use this device in the presence of explosive gas GENERAL CAUTION Be aware that the voltage and frequence of the electrical system are compatible with the electrical requirements of the battery charger CAUTION Never use a non OEM charger to charge the air sampler Use of improper charger may damaged the unit CAUTION Rev 9 Pg 2 vWReC e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SUMMARY Main Index SUMMARY renani enren 3 SECTION 1 BRIEF INSTRUCTIONS see 45 1 1 Brief instructions for SAS Super 100 180 and SAS Isolator esses 5 1 2 Brief operating instructions for DUO SAS Super 360 DUO SAS 1 5 SECTION 2 INTRODUCTION n 2 1 Principle of Surface Air te tro e iere cr e t eec siye eec PPS LEN eR Rede ed 6 2 2 Th basi2. ENTER button Rubber gasket Flashing LED during air sampling Infrared sensor for remote control Back lit alpha numeric visual A button Rev 9 VWRED e Pg 34 User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SAS Super 100 SAS Super180 SAS Isolator Bottom Panel 22 Protecting screw cap for tripod connection 23 Instrument Brief Instructions 24 Gasket 25 Instrument feet 7 3 Automatic switch off The instrument will automatically switch off after 4 5 minutes to save the battery The ON OFF switch black button must be pushed twice to switch on the unit to start a cycle if the automatic switch off has operated Rev 9 Pg 35 225 e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 8 ACCESSORIES 8 2 SAS Model Cat n 18198 B 19121 B Cat n Cat n Cat n Cat n Cat n Cat n Cat n 907 907 432 432 432 432 6 7 6 7 8 9 B Cat n 24584 A Cat n 907 8 Cat n 91746 Cat n 91796 SAS Super 100 Contact SAS Super 180 Contact SAS Super 100 Petri SAS Super 180 Petri SAS Super Isolator 100 Contact SAS Super Isolator 180 Contact SAS Super Isolator 100 Petri SAS Super Isolator 180 Petri DUO SAS Super 360 Contact DUO SAS Super 360 Petri DUO SAS Super 360 Isolator Contact
3. SAS SUPER 100 180 SAS ISOLATOR DUO SAS 360 DUO SAS ISOLATOR INSTRUCTIONS MANUAL Carefully read this Manual before operating your instrument This document is the property of VWR International PBI srl Milan It may not be duplicated or distributed without the owner authorisation REFERENCES 1987 Guideline on Sterile Drug Products produced by Aseptic Process ACGIH Guideline for the Assessment of Bioaerosol in the Indoor Environment ASTM Draft Protocol Committee D22 05 06 USP 23 NF 18 8 Supplement 1116 May 1998 Microbiological Evaluation of Clean Rooms and other Controlled Environments EU Guide for GMP Manufacture of Sterile Medicinal Products Control of Medicines and Inspection CEN TC 243 Norms for Clean Room Technology For any further information please contact United States BIOSCIENCE INTERNATIONAL Innovative Microbiology Products 11333 Woodglen Drive Rockville MD 20852 Telephone Telephone 301 231 7400 Fax 301 231 7277 E mail BioInfo Biosci Intl com United Kingdom Cherwell 7 amp 8 Launton Business Centre Murdock Road Bicester 26 4XB Telephone 44 0 1869 355 500 Fax 44 0 1869 355 545 E mail sales cherwell labs co uk Rev 9 September 2012 User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator INSTRUCTIONS MANUAL READ CAREFULLY THIS HANDBOOK
4. AIR SAMPLER IDENTIFICATION SHEET ACCORDING TO ISO EN 17025 According to the Document ISO EN17025 to apply the current Good Laboratory Practice it is necessary to identify each instrument with a specific sheet An example is here reported ISO EN 17025 Instrument Identification Sheet Form n XXXX Date XXXX Company Institute XXXX Laboratory XXXX Name of Instrument Microbiological Air Sampler Model SAS SUPER 100 Serial Number XXXX Name of Producer VWR Internaitonal PBI srl Name of Distributor XXXX Condition at the moment of arrival X new x used x reconditioned Date of Arrival Date of initial use Usual collocation Room XXXX Servicing details See servicing POS Instrument to be calibrated Periodic Calibration X Yes X No X monthly X quarterly X Yearly Instrument to be validated X Yes X No Periodic Validation X half year X yearly VWRED e Rev 9 Pg 37 User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 10 SAS ISOLATOR SAS SUPER ISOLATOR SCHEMATIC EXTERNAL INTERNAL Lemo style connectors inc Fixed coupler male amp __ one female cable plug to allow quick connecudisconnect 5 5 Sampling head inc Motor fan assembly Small metal feet so assembly stands off base All op
5. C CI 1 216 1 000 20 a Air sampler SAS SUPER 100 Serial Number 1210 was positioned in AREA A on site Al and programmed for a total air volume of 1000 litres in one run starting after 5 minutes from the switching on of the instrument b Air sampler SAS SUPER 100 Serial Number 1211 was positioned in AREA A on site A2 and programmed for a total air volume of 1000 litres in five times 200 litres during each run with interval times of 30 minutes c Air sampler SAS SUPER 100 Serial Number 1213 was positioned in AREA A on site and programmed for a total air volume of 1000 litres in four times 250 litres during each run with interval times of 30 minutes d Air sampler SAS SUPER 100 Serial Number 1214 was positioned in AREA B on site B1 and programmed for a total air volume of 1000 litres in four times 250 litres during each run with interval times of 30 minutes e Air sampler SAS SUPER 100 Serial Number 1215 was positioned in AREA B on site B2 and programmed for a total air volume of 1000 litres in five times 200 litres during each run with interval times of 30 minutes Rev 9 Pg 28 VWRESD User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator f Air sampler SAS SUPER 100 Serial Number 1216 was positioned in AREA C on site Cl and programmed for a total air volume of 1000 litres in one run starting after 20 minutes from the switching on of the instrument 6 7
6. Final operations Switch off the ON OFF Main switch black button The unit will switch off automatically after 4 5 minutes if the switchg off option has not been disabled for Infrared Remote Control use 6 8 Calculation of results 6 8 1 Colony Forming Units per 1000 litres of air The number of organisms counted on the surface of the Contact Plate must first be corrected for the statistical possibility of multiples particles passing through the same hole The statistical formula is taken from work by J Maker Correction Tables are given below for both the 55 mm standard Contact head the 84 mm Maxi Contact head and the Petri head The probable count Pr is then used to calculate the Colony Forming Unit CFU per cubic metre of air sampled Rev 9 Pg 29 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator CORRECTION TABLE TO ADJUST COLONY COUNTS FROM 219 HOLE IMPACTOR USING 55 mm CONTACT PLATES OR 90 mm PETRI PLATES MPN Mou MPN on MPN MPN d MPN MPN Pr Pr Pr Pr Pr Pr r r r 1 1 38 42 75 92 112 56 149 249 186 412 2 2 39 43 76 93 113 158 150 252 187 418 3 3 40 44 77 95 114 160 151 255 188 425 4 4 41 45 78 96 115 162 152 258 189 432 5 5 42 46 79 98 116 65 153 261 190 439 6 6 43 48 80 99 117 67 154 265 191 447 7 7 44 49 81 101 118 169 155 268 192 455 8 8 45 50 82 102 119 171 156 271 193 463 9 9 46 51
7. SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 4 10 2 Remote control The SAS microbiological air samplers can be used with an optional Infrared Remote Control cod 18950 With the remote it is possible to start the sampling cycle from distance When the remote control is going to be used the autoswitch off timer must be disable following the procedure described in the paragraph 4 8 3 The automatic switch off option will be re activeted by switching off the air sampler with the Main Switch black button When the auto switch off option is disabled remember to switch off the instrument after use to save the battery 4 10 3 SAS Software The SAS microbiological air samplers can be used with an optional Software cod 89996 With the SAS Software it is possible to download the sampling information to a PC through the RS 232 SAS cable cod 26025 The data can be stored in the PC printed and exported in xls or pdf format 4 10 4 SAS Printer The SAS microbiological air samplers can be used with an optional printer cod 89960 With the SAS printer it is possible to print the sampling information directly to the SAS printer Rev 9 Pg 22 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 5 MAINTENANCE 5 1 Air Sampler Disinfection The SAS SUPER 100 SAS SUPER 180 DUO SAS 360 SAS Isolator DUO SAS Isolator do
8. SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 4 OPERATIONS AND MENUS 4 1 Operations Push the Main switch black button to switch on the unit The Main switch black button must be pushed twice to switch on the unit if the automatic switch off timer operated when last used Press CLEAR at any time to return to the initial START FOR main screen The air sampler is provided with eight fixed air volumes Standard Mode stored in the memory and eight programmable air volumes User Mode selectable by the operator The selection of the volume is made by pressing the or V buttons when the program is in the relevant sub menu The following volumes of air are suggested Contaminated areas communities processing rooms etc 10 200 litres of air Normal areas laboratory benches houses etc 200 500 litres of air Sterile or high risk areas clean rooms operating theatres etc 500 1000 litres of air 4 2 Start with the same air volume as the previous sample SAS SUPER 100 SAS SUPER 180 amp SAS ISOLATOR Switch on the SAS with the black on off switch after the initial presentation the main screen will be displayed START FOR XXX MM DD YY HH MM Push the START button to start a sampling cycle with the displayed volume of air XX XXX MM DD YY HH MM DUO SAS SUPER 360 DUO SAS Isolator Switch on the SAS with the black
9. 83 104 120 73 157 275 194 471 10 10 47 53 84 106 121 76 158 278 195 480 11 11 48 54 85 107 122 78 159 282 196 489 12 12 49 55 86 109 123 180 160 286 197 499 13 13 50 57 87 110 124 182 161 289 198 508 14 14 51 58 88 112 125 85 162 293 199 519 15 15 52 59 89 114 126 87 163 297 200 530 16 17 53 60 90 116 127 89 164 301 201 542 17 18 54 62 91 117 128 192 165 305 202 554 18 19 55 63 92 119 129 194 166 309 203 567 19 20 56 64 93 121 130 96 167 313 204 580 20 21 57 66 94 122 131 99 168 317 205 595 21 22 58 67 95 124 132 201 169 322 206 611 22 23 59 69 96 126 133 204 170 326 207 627 23 24 60 70 97 128 134 206 171 331 208 646 24 25 61 71 98 130 135 209 172 335 209 666 25 26 62 73 99 131 136 212 173 340 210 687 26 28 63 74 100 133 137 214 174 344 211 712 27 29 64 76 101 135 138 217 175 349 212 739 28 30 65 77 102 137 139 220 176 354 213 770 29 31 66 78 103 139 140 222 177 359 214 807 30 32 67 80 104 141 141 225 178 365 215 851 31 33 68 81 105 142 142 228 179 370 216 905 32 34 69 83 106 144 143 231 180 375 217 978 33 36 70 84 107 146 144 234 181 381 218 1088 34 37 71 86 108 148 145 237 182 387 219 1307 35 38 72 87 109 150 146 240 183 393 36 39 73 88 110 152 147 243 184 399 37 40 74 90 111 154 148 246 185 405 colony forming units counted Pr probable count Rev 9 Pg 30 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator CORRECTION TABLE TO ADJUST COLONY COUNTS FROM A 401 H
10. HH MM Once the sampling volume to be modified has been reached press the ENTER button to confirm The display will show the old volume and the new volume XXX 0000 MM DD YY HH MM Press the Y or A button to select the first digit and press ENTER to confirm XXX X000 MM DD Y Y HR MM Press the Y or A button to select the second digit and press ENTER to confirm Repeat the previous step until the last digit when the last digit is confirmed the new volume will be memorized and added to the list of eight user selectable volume If you want to start a sampling cycle with the new value you have to enter the User mode and select it by using the procedure described in paragraph 4 3 Rev 9 Pg 14 VWRED User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 4 6 Delay mode function With this function it is possible to delay the beginning of the sampling cycle If a delay has been selected the sampling cycle will begin after the selected delay time Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the DELAY MODE MM DD YY HH MM Press the ENTER button the delay time is displayed DELAY 1 MIN MM DD YY HH MM Select the desired delay time with the W or A button The selectable delay v
11. not require special care and maintenance procedures Do not subject the air sampler to sudden shock Clean the air sampler with a soft damp cloth and a mild detergent Do not use harsh abrasives Do not submerge in water do not pour or spray water directly on the air sampler Do not use acetone or other volatile solvents for cleaning x Do not use chlorine or fluorine based products to disinfect the stainless steel head Body of the Sampler The plastic body of the unit can be wiped with a mild chemical agent and disinfected with alcohol wipe or swab Contact Plate Housing Head The unit complete with the sampling head can be disinfected using a disinfecting aerosol e g Isopropyl alcohol sterile spray cod 18223 held at about 30 cm from the head when the fan is running 30 seconds are sufficient to disinfect the air path XPauvo 30 cm 30 Seconds X Cover of the Head The sampling head with its protective plastic lid should be autoclaved at a minimum temperature of 121 C for at least 15 min following the good sterilization practice Alternatively the sampling head can be sanitised by treating the inside and outside surfaces with a disinfecting alcohol wipe or swab 5 2 Validation and Calibration The motor speed and consequently the air flow are related to the electrical power supply motor bearing conditions etc It is therefore suggested that the unit should be validated at least every six
12. removed from 2 A case and used D 2 FLOW DIAGRAM sterile spray Air lock a I AN BIO SAFE CARRIER Bic Safe Carrier disinfected externally joue camo SAS body using Dec Ahcl sterile spray m SAS placed into disinfected Bio Safo Carrior using Dec Ahol at 121 C for 20 E sterile spray 6 2 Use of the air sampler in Clean Areas The sampling head should be autoclaved and the body of the unit wiped with a mild chemical agent Note The air sampler is a scientific instrument and its surface should be disinfected using an aerosol and not a liquid The unit should be protected with a sterile bag and then transferred into the Clean Area It is important to wear sterile gloves when handling the Contact Plates and the head of the air sampler to prevent bacteriological contamination Use double packed gamma irradiated Contact Plates to avoid possible clean environment contamination For more details see Application Notes N 15 31 55 63 68 83 95 99 Rev 9 Pg 26 vWReCO User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 6 3 Contact Plates preparation It is important that the volume of media dispensed into a Contact Plate is constant for the type of plate used An excess volume can create a convex surface that is too high and which may touch the inside of the sampler head An insufficient volume will create a concave surface u
13. with a new one and connect the new battery 6 Reassemble the control block to the instrument body in the reverse order Be sure the rubber sealing ring Position 5 is correctly positioned A 9 w Rev 9 Pg 24 VWRES e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 5 5 Troubleshooting PROBLEM SUGGESTED ACTION Unit does not start Push ON OFF switch black button The ON OFF main switch must be pushed twice to switch on the unit if the automatic switch off operated at the end of the last cycle Recharge battery Check the battery charger Replace battery Battery discharges after few minutes working Recharge battery Check the battery charger Replace the charger LOW BATTERY message Recharge battery Infrared remote switch doesn t switch on the unit Replace infrared remote battery Microbiological media is dehydrated after sampling Control expiration date of media and that the agar is not dehydrated before sampling VWRED e Rev 9 Pg 25 User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 6 SAMPLING PROCEDURE 6 1 Contact Plate handling a Disinfect the cover of the air sampler at the beginning of each sampling program treating the inside and the outside part with a disinfecting alcohol wipe
14. 346 33 34 73 80 113 133 53 192 93 263 233 348 34 35 74 82 114 134 54 194 94 265 234 351 35 37 75 83 115 135 55 196 95 267 235 353 36 38 76 84 116 137 56 197 96 269 236 355 37 39 77 85 117 138 97 199 97 271 237 358 38 40 78 87 118 140 58 201 98 272 238 360 39 41 79 88 119 141 59 202 99 274 239 363 40 42 80 89 120 142 60 204 200 276 240 365 9 31 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator Example of calculation of results y Pr 1000 V Where V Volume of sampled air 200 litres of air r Colony Forming Units counted on 55 mm Contact Plates 67 Pr Probable count obtained by positive hole correction 80 Colony Forming Units 1000 litres 1 cubic metre of air 80 1000 400 CFU per 1000 litres of air 1000 litres 1m 200 To express the final result in CFU f multiply the CFU m value by 0 02832 6 9 Data recording data related to instrument identification operator s name sampling site date and hour of sampling type of media CFU may be reported on the specific software SAS software Rev 9 Pg 32 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 7 SPECIFICATIONS 7 1 Specifications Principle Air borne micro organisms are collected on microbiological agar by impac
15. 60 DUO SAS 360 Isolator 2 3 The practical use of contact plates THE PRACTICAL USE OF CONTACT PLATES FOR AIR CONTROL SAS SUPER FLOW SCHEME 5 Remove the aspirating head Insert an identified closed and prepared Contact Plate and remove plate lid Place the aspirating head back onto the sampler Select the required air volume and start the unit The air flow is directed onto the agar surface of the Contact Plate At the end of the cycle remove the aspirating head Close and remove the Contact Plate Incubate the Contact Plate Count the colonies record the results on the microbiological air sampling report and analyse the results fou 9o coe pA 2 4 SAS models available 2 4 1 SAS SUPER 100 and SAS SUPER 180 Two instruments for two different applications The two air samplers have the same performances the only difference between them is the airflow aspiration rate SAS SUPER 100 100 litres of air per minute SAS SUPER 180 180 litres of air per minute The SAS SUPER 180 is appropriate for use in Clean Rooms and other applications demanding low sampling times In Clean Rooms for example it is important to test a larger volumes of air because the microbial air contamination is very low The SAS SUPER 180 reduces the required time to obtain a sample with an important saving of time Both SAS SUPER 100 and SAS SUPER 180 are identified by the SAS SUPER logo on the right side of the ins
16. DUO SAS Super 360 Isolator Petri 8 2 Accessories and spare part list 15931 15503 19123 19117 19119 70003 5214 5215 89722 90724 91213 Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n Cat n 9248 86603 20153 8143 86751 25094 8950 8200 89960 20093 20094 55 mm aluminium certified drilled head 55 mm stainless steel certified drilled head 84 mm aluminium certified drilled head adaptor for Maxi Contact Plate 90 mm aluminium standard Petri dish head adaptor 90 mm stainless steel standard Petri dish head adaptor Autoclavable plastic cover for sampling head Table tripod Floor tripod Fixed stainless steel tripod Extendible stainless steel tripod Stainless steel stand Normal battery charger 110 Volt Fast battery charger 220 Volt Aluminium carrying case for SAS Super 100 180 Soft carrying case for SAS Super 100 180 Aluminium carrying case for DUOSAS Soft carrying case for DUOSAS Infrared remote control IN OUT MH Battery pack SAS Printer Printer to be connected to SAS Super 100 Installation Qualification manual Operational Qualification manual 8 2 Control and Validation Systems Cat n 86666 Pyramid Rev 9 Pg 36 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 9 IDENTIFICATION SHEET
17. MBER OF RUNS 20 NC Select the desired number of cycles with the Y or A button The selectable values are 2 3 4 5 6 7 8 9 10 15 or 20 runs Press ENTER button to confirm the number of runs the single run volume is displayed SINGLE RUN VOL XXXX Select the desired digit with the Y or A button then press the ENTER button to skip to next digit When all the digits have been selected the total volume is displayed TOT L XXXX Press ENTER button to confirm NOTE Before running the multimode sampling set the aspirating mode to MULTIMODE see paragraph 4 8 2 4 7 1 Start a multimode sampling cycle Before running the multimode sampling set the aspirating mode to MULTIMODE see chapter 4 8 2 When the sampler aspiration mode is set to MULTIMODE the main display will show alternately the number of cycles and the volume of each cycle NC 02x1000 L MM DD YY HH MM START FOR MM DD YY HH MM Press the START button to run the multiple sampling the display will show the aspirating volume in the first row and the residual number of the cycle in the second row mE EE 02 CYCLES At the end of the run during the interval the number of residual cycles runs that are remaining are shown on the display 02 CYCLES DELANY Rev 9 Pg 16 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Is
18. OLE IMPACTOR USING 55 mm CONTACT PLATES OR 90 mm PETRI PLATES Coloni MPN Coloni MPN Coloni MPN Coloni MPN Coloni MPN Coloni MPN es Pr es Pr es Pr es Pr es Pr es Pr r r r r r r 1 1 41 43 81 90 21 144 61 206 201 278 2 2 42 44 82 92 22 145 62 207 202 280 3 3 43 45 83 93 23 147 63 209 203 282 4 4 44 47 84 94 24 148 64 211 204 284 5 5 45 48 85 95 25 150 65 212 205 287 6 6 46 49 86 97 26 151 66 214 206 289 7 7 47 50 87 98 27 152 67 216 207 291 8 8 48 51 88 99 28 154 68 217 208 293 9 9 49 52 89 100 29 155 69 219 209 295 0 0 50 53 90 102 30 157 70 221 210 297 11 11 51 54 91 103 31 158 71 223 211 299 2 12 52 56 92 104 32 160 72 224 212 301 3 3 53 57 93 106 33 161 73 226 213 303 4 4 54 58 94 107 34 163 74 228 214 305 5 15 55 59 95 108 35 164 75 230 215 307 6 16 56 60 96 110 36 166 76 231 216 310 7 7 27 61 97 111 37 167 77 233 217 312 8 8 58 63 98 112 38 169 78 235 218 314 9 19 59 64 99 114 39 170 79 237 219 316 20 20 60 65 00 115 40 172 80 239 220 318 21 22 61 66 01 116 41 173 81 240 221 321 22 23 62 67 02 118 42 175 82 242 222 323 23 24 63 68 03 119 43 177 83 244 223 325 24 25 64 70 04 120 44 178 84 246 224 327 25 26 65 71 05 122 45 180 85 248 225 330 26 27 66 72 06 123 46 181 86 250 226 332 27 28 67 73 07 124 47 183 87 251 227 334 28 29 68 74 08 126 48 184 88 253 228 336 29 30 69 76 09 127 49 186 89 255 229 339 30 31 70 77 110 128 50 188 90 257 230 341 31 32 71 78 111 130 51 189 91 259 231 343 32 33 72 79 112 131 52 191 92 261 232
19. al devices is regulated within the European Community by national regulations based on European Directive 2002 95 EC 2002 96 EC and 2003 108 EC pertaining to waste of electrical and electronic equipment WEEE The crossed out wheelie bin logo labelled on the equipment or on its packaging means that this product must be disposed separately form the other waste Because disposal regulation may differ from country to country For further informations please contact your supplier Rev 9 Pg 40 VWRED e
20. alues are 1 2 3 5 10 or 20 minutes Press ENTER button to confirm When a delay has been selected after the START button has been pressed a delay warning message will ODE LAN t This program is very useful for extending the time of sampling with the purpose of obtaining a more be displayed 4 7 Multimode function representative environmental sample in actual operating conditions The total air volume to be sampled is aspirated with two or more sub volume aspirations E g 1000 litres in ten runs of 100 litres at five minute intervals Before entering the MULTI MODE you should therefore decide a total volume of air to be sampled onto the Contact Plate b number of runs c interval time between runs See Application Note N 95 for more details Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the MULTI MODE MM DD YY HH MM Press the ENTER button the interval time is displayed Rev 9 Pg 15 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator INTERVAL TIME 05 MIN Select the desired interval time between runs with the Y or A button The selectable interval values are 5 10 15 20 25 30 or 60 minutes Press ENTER button to confirm the interval time the numbers of runs is displayed NU
21. ampling with several independent air samplers sese 28 6 7 Final operations ais 6 8 Calculation Of results itte tte tette re abere p HE e Pet to beh Deae eee 29 6 8 1 Colony Forming Units 1000 litres of air 29 6 9 Data recording d SECTION 7 SPECIEICATIONS TA Specifications esere eoni 7 2 SAS Components Description ze 7 3 Automatic Switch Off ecce stre ecrire teed 35 SECTION 8 ACCESSORIES ter RERO e ERU P rk pe Er pe tetbe tee b Dru ripe ipe tet tren Perkins 36 8 2 SAS Model 8 2 Accessories and spare part list eee ete tro 8 2 Control and Validation Systems canet dte eter ee ipi RTE SECTION 9 IDENTIFICATION SHEET is SECTION 10 SAS ISOLATOR eieeettire tete tete bre nbecstba a EEN A EEEE ese re Pose det aee dee Rev 9 Pg 4 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 1 BRIEF INSTRUCTIONS 1 1 Brief instructions for SAS Super 100 180 and SAS Isolator The air flow is displayed after the automatic presentation every time the instrument is switched on Press ON OFF switch Press START button to sample the same air volume of the last sampling cycle To change the air volume use the arrow buttons and select Standard Mode from menu R
22. arger to the battery socket on the SAS SUPER 100 or SAS SUPER 180 3 Connect the battery charger to the main supply 220 240 or 110 Volts verify that the LEDS on the charger are lit correctly see label on charger 4 The air sampler is recharged after 14 hours using the normal battery charger Revit Cat 19248 When the green led of the battery charger is lit disconnect the charger from the mains supply 6 At the end of operation disconnect the battery charger plug from the socket of the air sampler e NOTES a The correct metal hydrate battery charger must be used to charge the SAS b Ifthe air sampler has not been used for several weeks check the battery status c This battery pack using the SAS SUPER 100 sampler should provide approximately 5 7 hours of sampling time 50 less in SAS SUPER 180 d Before using for the first time or after a storage time of one month or more charge battery for 14 hours If you do not reach the rated battery capacity during initial use repeat the 14 hours charge process e Battery performance will degrade if the SAS is used in an out of specs environment f The Metal Hydrate battery has a self discharge rate and without use will lose about 2 of its charge per day g To prevent injury or burns do not allow metal objects to contact or short circuit the battery terminals h The Metal Hydrate battery may explode if disposed in fire Rev 9 Pg 21 VWRED User Manual
23. ast 14 hours before running the test SAS SUPER 100 SAS SUPER 180 AND SAS ISOLATOR After the ON OFF button is pressed an automatic visual presentation will be displayed In the following displays the characters XX indicates numerical figures INTERNATIONAL PBI S N 10 D XXXX Rev 5 XX XXXX PRESS ARROW FOR NEW MENU SELECT S SAS 100 L START FOR XXX MM DD YY HH MM Push the START button to start a sampling cycle with the displayed volume of air Lo ou MM DD YY HH MM The motor will run until the figures in brackets reach the same figure displayed to the right and then it will stop During the sampling the two red leds are flashing see position 9 and 20 in the picture of section 7 Rev 9 Pg 9 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator DUO SAS Super 360 DUO SAS Isolator After the ON OFF button is pressed an automatic visual presentation appears In the following display the entry of indicates numerical figures INTERNATIONAL PBI S N 10 D XXXX Rev 5 XX XXXX PRESS ARROW FOR NEW MENU SELECT DUOSAS 360 L SELECT HEAD Select the desires head by UP or DOWN arrows and press ENTER Push the START button to start a sampling cycle with the displayed volume of air XX XXX MM DD YY HH MM The motor will run until the figures in brackets reach the same figure as display
24. cidea ooo ert rr EE IR HEURE HIVE RESI reU pe toit trap eo be prie tees 6 2 3 The practical use of contact plates m 2 4 SAS models available zT SECTION 3 FIRST OPERATIONS 459 3 1 Preliminary Inspection acre ene ett Sri ea ie Dar ah ined ber 9 3 2 55 mm Contact Plates Holder 10 3 3 84 mm Maxi Contact Plates 3 4 Volume of agar m each plates pei ad anie tee ENE AETR NT 3 5 pod installation optional rere tet terrore 3 6 List of menu and utility sub menu SECTION 4 OPERATIONS AND MENUS 4 1 Operations 12 4 2 Start with the same air volume as the previous sample 2 12 4 3 Standard mode MUNG MOM oerte ocn lets EDU 13 4 4 User mode function 4 5 Program mode function iuo coercet credere tee id ee epe Heo ir e er Ve eco eben v eeu eR 14 4 6 Delay mode functiofi trt ICE NPE RU Ie FERE RIGEN ERREUR E ERR 15 4 7 Multimode function 4 7 1 Start a multimode sampling cycle eese teen nennen 16 4 8 Utility mode function eee eer ge tenter tie mere e terii e at 17 4 8 1 Set time 4 8 2 Mode 4 8 3 Set autoswitch AB iA PrDU oe ese cere esteso metier E e LINDA 4 8 5 Display record cete ctn e ER dare e end
25. ctable volumes can be selected form the User Mode while the selectable volumes can be modified using the Program mode function describe in the next paragraph Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the USER MODE MM DD YY HH MM Press the ENTER button now you can scroll the eight sampling volumes user defined Rev 9 Pg 13 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator S PROG XXXX MM DD YY HH MM Once the desired sampling volume has been reached press the ENTER button to confirm The instrument is now ready to sample the selected volume of air Press the START button to begin the sampling cycle XXX START FOR MM DD YY HH MM The total number of available USER MODE programs is eight The maximum volume of air for each sampling cycle is 1999 litres 4 5 Program mode function Using this procedure it is possible to memorise up to eight different volumes from 1 to 1999 litres of air See chapter 4 4 Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the PROGRAM MODE MM DD YY HH MM Press the ENTER button now you can scroll the eight user selectable sampling volumes S PROG XXXX MM DD YY
26. de and press ENTER to confirm and exit This setting will be stored in memory even after the instrument is switched off Rev 9 Pg 17 VWRED User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 4 8 3 Set autoswitch To save battery consumption the SAS automatically switches off after 4 minutes The Set autoswitch option is used to enable or disable the automatic switch off From the UTILITY MODE select the SET AUTOSWITCH function SET AUTOSWITCH MM DD YY HH MM Press ENTER and or Y button to change between the two options AUTOSWITCH ON AUTOSWITCH OFF MM DD YY HH MM MM DD YY HH MM Select the desired options and press ENTER to confirm and exit This setting will be lost after the instrument is switched off 4 8 4 Print The SAS can be connected with a RS232 cable to the SAS printer cod 89960 or to a Personal Computer with the SAS Software cod 89996 This function is used to start the data transfer form the SAS to the external device From the UTILITY MODE select the PRINT function PRINT MM DD YY HH MM Press ENTER to start sending the data the display will show SENDING DATA For more information see the printer or software user manual 4 8 5 Display record The last 99 samples are memorized in the file DISPLAY RECORD Each sample is identified in chronological order and shows the date time operato
27. e irte rtis pere v 18 4 8 6 Clear record s 4 8 7 GAN SUAGC E THenttby eiecti misce tetun dieit a RRA 4 8 9 Sampling Site ceo tetro 20 4 8 10 Calibration due OPTIONAL FEATURE 20 4 9 List of all system 4 A TO e cu C 4 10 1 Battery charger 4 10 2 Remote control SAS ccrtc BIRD IIT 4 10 4 SAS Printer ear I IR TTE Te E a PI RE PEREAT FU SECTION 5 MAINTENANCE ds Sb Aur Sampler Distt eC Ons aeo crono P URSI Gn eO deep eus 23 5 2 Validation and Calibration erri y p PE ERES 200 pide E PUTENT II FUP 23 5 3 Performance routine monitoring 5 4 Battery replacement x 5 5 Troubleshooting petri CEP E EN eges 25 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 6 SAMPLING PROCEDURE 2 26 6 1 Contact Plate handling 26 6 2 Use of the air sampler in Clean Areas 226 6 3 Contact Plates preparation 27 6 4 Adjustable Contact Plate holders nd 6 5 Sampling time required for different air volumes 27 6 5 Total Bacterial Count and or micro organisms identification by dilution sss 28 6 6 Multi Point S
28. e resulting laminar air flow is directed onto the agar surface of a RODAC Plate or a Petri dish containing medium for the microbiological analysis When the selected sampling cycle is completed the plate is removed and incubated The organisms are then visible to the naked eye and can be counted for the assessment of the contamination level 2 2 The basic idea The key features of the Surface Air System SAS are A use a simple and inexpensive Contact Plate SURFAIR PLATE RODAC for surface hands or air control These plates are very well known and easily available They can be purchased ready poured with different media B Tosample a known volume of air for a variable time to provide a range of sampling volumes C To aspirate air in a laminar flow pattern with sufficient velocity to impact organisms onto an agar surface D Toaccumulate data on the level of hygiene in each environment so that fluctuation can be monitored E Totake advantage of advanced electronics for more reliable results in different operating conditions F To have the flexibility to choose between 55 mm Contact Plates 84 mm Maxi Contact Plates or 90 mm Standard Petri Dishes G To apply cGLP and cGMP to air sampling operations H To organize sequential sampling to obtain a more representative sample under actual operating conditions Rev 9 Pg 6 VWRES e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 3
29. ed to the right and then it will stop During the sampling the two red leds are flashing see position 9 and 20 in the pictures in Section 7 3 2 55 mm CONTACT PLATES HOLDER ADJUSTMENT The Contact Plate holders can be adjusted using an allen screw in case the available plates slightly different in diameter from the standard 55 mm Contact Plate see the figure in the Chapter 6 4 3 3 84 mm MAXI CONTACT PLATES If high fungal contamination is expected in the sampled air Maxi Contact Plates are suggested This is because moulds tend to spread and consequently make counting difficult after incubation The 84 mm Maxi Contact Plates are inserted into the SAS SUPER 100 or SAS SUPER 180 using a special adaptor Cat n 19123 For more details see Application Note N 89 3 4 Volume of agar in each plate For a correct sampling efficiency it is important to respect the plate agar medium volume as recommended by the air sampler supplier e g 16 18 ml of agar medium in 55mm contact plate to obtain the correct impact of air on the agar surface Rev 9 Pg 10 VWRED User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 3 5 Tripod installation optional The SAS can be fixed to a table tripod or a floor tripod The threaded connection is located under the unit between the two front feet When not in use the screw is protected by a threaded plug 3 6 List of m
30. efer to the instruction manual to change settings Press ENTER to confirm selection Press arrows for menu selection to modify other pre set parameters STANDARD MODE USER MODE PROGRAM MODE DELAY MODE MULTI MODE UTILITY MODE Press ENTER to reach the sub menu of the chosen parameter Refer to instruction manual to change settings Press ON OFF switch at the end of sampling operations Press CLEAR each time you need to end an action Then the unit will come back to the initial configuration 1 2 Brief operating instructions for DUO SAS Super 360 DUO SAS Isolator Press the ON OFF switch black button When the display will show the message SELECT HEAD press ENTER Press UP or Down arrows to select the LEFT HEAD or the RIGHT HEAD or LEFT RIGHT HEAD Press ENTER to confirm selection Press START button to sample the same air volume as the last sampling time To change the volume of air or other parameters follow the given instructions for SAS Super 100 and SAS Super 180 Rev 9 Pg 5 vWReC e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 2 INTRODUCTION 2 1 Principle of Surface Air System The Surface Air System SAS encompasses several models which use the same principle Air is aspirated at a fixed speed for variable time through a head which has been machined with a series of small holes of a special design Th
31. enu and utility sub menu MENU DESCRIPTION START FOR XXX This message indicates the air sampler is ready to sample the volume shown STANDARD MODE With this function the air sampler can be set to select one of 8 fixed standard sampling volumes USER MODE With this function the air sampler can be set to select one of 8 programmable sampling volumes PROGRAM MODE With this function you can to modify the 8 programmable sampling volumes DELAY MODE With this function the air sampler can be set to start after a programmable delay MULTI MODE UTILITY MODE With this function the air sampler can be programmed to extend the total sampling time using sequential interval time sampling This function led to the following Sub Menu MENU SET TIME To adjust date and time SAMPLING SITE To identify the sampling point IDENTIFY To identify the operator s name LANGUAGE To display text in different languages CLEAR RECORD To clear the recorded sampling data DESCRIPTION DISPLAY RECORD To show the recorded sampling data PRINT To export the recorded sampling data AUTO SWITCH OFF To disconnect the automatic switch off when the Infrared Remote is used MODE To select the aspiration mode between single and multiple CALIBRATION DUE To check the calibration due date vWReC 9 Pg 11 User Manual SAS Super 100 180
32. erator controls Rev 9 Pg 38 VWRES e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator WARRANTY WARRANTY CONDITIONS VWR International PBI srl warrants this product to be free of defects in materials and functionality The repairs will be carried out by VWR International PBI srl or by authorized dealers as follows Faulty components free replacement The labour is always at customer s expense The equipment will be sent at buyer s expenses and risk and the return will be ex works This warranty does not apply to damages resulting from tampering abnormal use and installation and does not cover transport damages VWR International PBI srl shall not be liable for any commercial damages whether incidental consequential or otherwise derived from the use of instruments ATTENTION TO OUR NEW CUSTOMER As a user of our equipment you are covered for a 12 months guarantee for all spare parts as a result of mechanical failure of the machine during normal use In order to provide you with first class service it is essential for us to know all your references and which equipment you have We kindly ask you therefore to fill in the attached guarantee and return the card to us by first class mail Rev 9 Pg 39 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator USER INFORMATIONS The disposal of electric
33. h colony count is expected if inhibitors are expected in the air being sampled or if the micro organisms are to be incubated at different temperatures the dilution method should be applied The total amount of agar of the Contact Plate is aseptically transferred to a Stomacher bag sterile diluent is added and the Stomacher treatment is applied for one minute The usual plating technique and or identification procedures are applied to the processed diluent 6 6 Multi Point Sampling with several independent air samplers A progressive simultaneous operation monitoring in a closed environment can be organised using several SAS Super 100 air samplers programmed with different starting delay times and sequential interval steps The air samplers can be positioned on a different walls using a support arm cat n 19834 to allow 45 inclination At the end of operation the air samplers are collected for Contact Plate transfer and data export to paper by printer or PC This type of organisation allows a to reduce staff labour involved in sampling b to reduce microbial contamination risk because the enclosed environment is monitored in operation c to obtain complete written report on air sampling AREA Position SAS s n Single Air Total Air Sub Sampling Start Volume Volume Sample interval delay number A Al 1 210 1 000 5 A2 1 211 200 1 000 5 30 A3 1 213 250 1 000 4 30 B B1 1 214 250 1 000 4 30 B2 1 215 200 1 000 5 30
34. nsuitable for representative colony growth using SAS System The volume of agar in a 55 mm Contact Plate should be 16 18 ml and in a 84 mm Maxi Contact Plate 24 26 ml For more details see Application Note N 78 6 4 Adjustable Contact Plate holders The SAS SUPER 100 180 DUO SAS 360 SAS ISOLATOR DUO SAS Isolator air samplers are designed for use with standard Contact Plates The plate holders are adjustable to allow different brands of Contact Plate to be fitted Adjustable plate holders in the sampler head allow the operator to use contact plates from many sources without concern for slight differences in plate size 6 5 Sampling time required for different air volumes SAS SUPER 100 SAS SUPER 180 LITRES OF AIR TIME REQUIRED LITRES OF AIR TIME REQUIRED 50 30 seconds 90 30 seconds 100 minutes 180 minute 200 2 minutes 360 2 minutes 300 3 minutes 540 3 minutes 400 4 minutes 720 4 minutes 500 5 minutes 900 5 minutes 600 6 minutes 1000 5 minutes 30 seconds 700 7 minutes 800 8 minutes 900 9 minutes 1000 10 minutes Rev 9 Pg 27 VWRED User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 6 5 Total Bacterial Count and or micro organisms identification by dilution In normal conditions if sampling is correctly performed the colonies are easily counted and collected for identification on the agar surface of the Contact Plate If very hig
35. olator 4 8 Utility mode function With this function it is possible to modify all the settings of the air sampler The UTILITY MODE is divided into ten sub menus Set time Mode Set Autoswitch Print Display Record Clear Record Language Identify Sampling site Calibration due Always start from UTILITY MODE to reach one of these SUB MENU Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the UTILITY MODE MM DD YY HH MM Press the ENTER button the first sub menu is listed 4 8 1 Set time From the UTILITY MODE select the SET TIME function This option is used to program day month year and time of the day SET TIME MM DD YY HH MM Press ENTER and or Y button to change month Press ENTER and A or Y button to change day Press ENTER and A or Y button to change year Press ENTER and A or Y button to change hours Press ENTER and A or Y button to change minutes Press ENTER to confirm and exit 4 8 2 Mode From the UTILITY MODE select the MODE function This option is used to select the aspirating mode between the SINGLE MODE and MULTIMODE MODE MM DD YY HH MM Press ENTER and or Y button to change between SINGLE MODE and MULTIMODE MULTI MODE SINGLE MODE MM DD YY HH MM MM DD YY HH MM Select the desired aspirating mo
36. on off switch after the initial presentation the head selection screen will be displayed SELECT HEAD Select the desires head by or Y arrows and press ENTER the main screen will be displayed START FOR XXX MM DD YY HH MM Rev 9 Pg 12 vWReC e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator Push the START button to start a sampling cycle with the displayed volume of air MM DD YY HH MM 4 3 Standard mode function The Standard mode function allows the operator to select a sampling volume from a list of eight standard volumes Switch on the instrument and wait until the main screen is displayed START FOR XXX MM DD YY HH MM Press the W or A button to reach the STANDARD MODE MM DD YY HH MM Press the ENTER button now you can scroll the eight sampling volume The selectable volumes are 10 20 30 50 100 200 500 1000 liters S PROG XXXX MM DD YY HH MM Once the desired sampling volume has been reached press the ENTER button to confirm The instrument is now ready to sample the selected volume of air Press the START button to begin the sampling cycle START FOR XXX MM DD YY HH MM 4 4 User mode function If the operator wish to use volumes other than the standards volumes up to 8 additional volumes can be programmed into the unit The user sele
37. or swab b The cover with its protective plastic lid can be autoclaved at 121 C for 15 minutes If this procedure is to be repeated regularly a s s head is recommended It is therefore useful to have available several heads ready to use Alternatively the unit can be sanitised using a disinfecting aerosol at about 30 cm from the head when the fan is running Thirty seconds are sufficient to disinfect the air path c Remove the cover of the air sampler by unscrewing it holding the edge of the cover Avoid touching the inside and outside of the drilled area d Insert a closed filled Contact Plate into the retaining slots and then remove its lid Avoid contamination from droplets and aerosol infection e Replace the sampling head and start the air aspiration cycle At the end of the cycle unscrew the sampling head replace the Contact Plate lid and remove the Contact Plate f Be sure each Contact Plate is identified with the appropriate sample data g Incubate the Contact Plate for the specified time at the appropriate temperature h At the end of incubation count the number of visible organisms C F U and relate this number to the volume of air sampled Clean room Air lock Ante room SAS placed into Bio Safe Carrier case li SAS head and transferred to other gt Biosafecarrier gt E gt autoclaved at clean room or airlock 121 for 20 mins SAS SUPER 100 t SUP So SAS
38. r site and volume of air sampled From the UTILITY MODE select the Display record function DISPLAY RECORD MM DD YY HH MM Press ENTER to see the memorized values 001 MM DD HH MM ID SITE VOL OP Rev 9 Pg 18 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator The following parameter are recorded Progressive number Month and Day Time Identification Site Sampling Volume Option If there is no data stored in memory the display will show x OK ck ck ck ck Ok ck ch ch ck ck x OK ck ck ck ck Ok Gk ch ch ck ck 4 6 6 Clear record This option is used to delete all the data memorized in the DISPLAY RECORD Before starting this procedure please be certain that existing data is not required or that it has been downloaded From the UTILITY MODE select the Clear record function CLEAR RECORD MM DD YY HH MM Press ENTER to delete all the stored data the display will show the resetting status RESETTING b 4 8 7 Language The text of the Menus may be selected choosing from different languages From the UTILITY MODE select the LANGUAGE function LANGUAGE MM DD YY HH MM Press ENTER to list all the available languages ENGLISH Use the or W button to select the desired language and press ENTER to confirm There are six available languages English French Spanish German Port
39. re With this function the user can see when the next calibration should be performed From the UTILITY MODE select the CALIBRATION DUE function CALIBRATION DUE MM DD YY HH MM Press ENTER to see the next calibration date NEXT CAL MM YYYY The display show the month in which the calibration should be performed One month before the calibration due the SAS will display the following warning message CALIBRATION DUE MM YYYY When this message is displayed press ENTER to acknowledge When the calibration has expired the SAS will display the following warning message Rev 9 Pg 20 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator CAL EXPIRED When this message is displayed press ENTER to acknowledge The calibration timer can be reset only by a qualified technician 4 9 List of all system message Besides the messages described in the previous paragraph the following messages can be displayed LOW BATTERY The battery of the instrument must be recharged MOTOR ERROR Motor not working or not connected OFF The instrument is switching off NEXT CAL The calibration due is approaching CAL EXPIRED The calibration due is expired 4 10 Accessories 4 10 1 Battery charger When the message LOW BATTERY is flashing the air sampler must be recharged 1 Switch off the unit Connect the smaller plug of the battery ch
40. sary to collect micro organisms to a size of 1 micron the 401 holes special aspirating head should be used This head has smaller holes to increase the velocity of particles and achieve a 100 capture efficiency on particles down to 1 micron in size Acoustic signal An acoustic signal alerts the operator when air sampling is completed 3 0 Volts Flat Battery Date and hours are activated even when air sampler is switched off by a 3 0 V flat battery The instrument should be switched on during the 3 0 V flat battery replacement to avoid loosing date and hours Rev 9 Pg 33 VWRE_ e User Manual SAS Super 100 180 SAS Super Isolator 100 180 SAS 360 DUO SAS 360 Isolator 7 2 SAS Components Description SAS Super 100 SAS Super 180 SAS Isolator Contact Plate Chamber 1 Adjustable holders to accommodate Contact Plates of different diameters 2 Screws not to be touched by the operator 3 Motor Fan 4 Contact Plate fixing spring SAS Super 100 SAS Super 180 SAS Isolator Upper Panel Feet for sampling in upright position ON OFF switch black button 5 Printer connection Battery charger connection Flashing LED during air sampling 6 10 Instrument Serial Number 11 CE mark SAS Super 100 SAS Super 180 SAS Isolator Front Control Panel 12 13 display 14 Y button START button CLEAR button
41. tion produced by aspiration Nominal Air Flow Rate SAS SUPER 100 100 litres of air per minute SAS SUPER 180 180 litres of air per minute SAS ISOLATOR 100 or 180 litres of air per minute DUO SAS 360 DUO SAS Isolator 180 litres of air per minute each head Range of Air Sample Volume 10 20 30 50 100 200 500 1000 litres are permanently memorised Standard Mode up to eight other volumes can be selected and memorised by the operator User Mode Maximum volume of air per cycle is 1999 litres Voltage 8 4 Volts 2700 mAh Temperature Working Range from 4 C to 40 C Humidity Working Range from 0 to 80 RH Battery Pack Rechargeable Nickel Metal Hydride without memory effect Battery Autonomy Aspiration of more than 40 000 litres of air Motor 6 Volts 2 8 Watts Size 105x110x290 mm Weight 1750g Construction Aspirating Head Aluminum or stainless steel Motorfan PPO Noryl Housing Polyurethane resin Sampling Efficiency The SAS SUPER 100 in a controlled environment with aerosol of known particle size is suitable to collect micro organisms down to 1 microns in size Considering the normal range of environmental micro organisms is between 4 and 20 microns the SAS SUPER 100 is suitable for all normal air monitoring testing V LACH Journal of Hospital Infection 1985 6 102 107 This condition is obtained using the standard 219 holes aspirating head If for special applications or research it is neces
42. trument The SAS SUPER 180 air sampler is recognizable by the SAS SUPER 180 logo that appears on the display when the operator turns on the unit Rev 9 Pg 7 vWReC User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator 2 4 2 DUO SAS SUPER 360 The instrument is based on the SAS Super 100 180 model but it includes two heads that can work alone or together 2 4 3 SAS ISOLATOR Critical air monitoring in isolator and barrier environments It consists of a programmable unit which remains outside the controlled area and an independent sampling head which uses RODAC Contact Plates or Standard 90 mm Petri dishes See Section 10 2 4 4 DUO SAS SUPER 360 f The instrument is based on the SAS Super Isolator model but it includes two heads that can work alone or together Rev 9 Pg 8 VWRES e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator SECTION 3 FIRST OPERATIONS 3 1 PRELIMINARY INSPECTION The apparatus is subject to specific quality tests before shipment and it is carefully packed to avoid possible damage during transit However a visible check should be carried out as soon as possible to determine any transit damage This must be reported immediately The following procedures should be followed to check that the unit is working properly The battery pack of the instrument must be charged for at le
43. twelve months The validation of the air flow rate should be done by a certified anemometer in a wind tunnel This type of certification may be performed by the manufacturer or an official distributor Rev 9 Pg 23 vWReCO User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator When an Official Calibration is requested a certified anemometer must be used to meet the required traceability characteristics i e FDA inspections require a Calibration Certificate that is traceable to know standard such as the NIST 5 3 Performance routine monitoring The SAS SUPER 100 SAS SUPER 180 DUO SAS SUPER 360 SAS Isolator DUO SAS Isolator should be checked monthly bimonthly depending on how often they are used using the Pyramid units to monitor that they are working correctly and their performances has not been affected by bad handling or damage NOTE The Pyramid is to be used for internal validation only i e to verify the correct working conditions of the air sampler 5 4 Battery replacement 1 Take the three caps Position 1 from the lower part of the control panel 2 Unscrew the three fixing screws Position 2 that fix the control block 3 Slide the control block Position 3 from the instrument body Position 6 Caution disconnect battery pack immediately 4 Disconnect the two pin connectors Position 4 from the battery 5 Take out the old battery replace it
44. uguese and Italian 4 8 8 Identify This option is used to identify the operator This should be changed if different operators uses the sampler and especially if the data is to be printed From the UTILITY MODE select the IDENTIFY function IDENTIFY MM DD YY HH MM Press ENTER to modify the identification code Rev 9 Pg 19 VWRED e User Manual SAS Super 100 180 SAS Super Isolator 100 180 DUO SAS 360 DUO SAS 360 Isolator IDENTIFY XXXX Select the desired character with the or Y button and confirm with ENTER Select the next desired characters and confirm them by pressing ENTER The selected value is stored in the memory and it will be kept also after the instrument has been switched off 4 8 9 Sampling Site The site identification should be changed for samples taken at different sites especially if the results are to be printed From the UTILITY MODE select the SAMPLING SITE function SAMPLING SITE MM DD YY HH MM Press ENTER to modify the sampling site SAMPLING SITE XXXX Select the desired character with the or Y button and confirm with ENTER Select the next desired characters and confirm them by pressing ENTER The selected value is stored in the memory and it will be kept also after the instrument has been switched off 4 8 10 Calibration due OPTIONAL FEATURE The SAS air sampler can be equipped with a calibration reminder featu
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