User`s Manual
Contents
1. Full Name Position Labconco P N 36960 04 Rev G ECO F572 34 Product Service 1 800 522 76582. USA 1338000 Power Cord with NEMA 6 15P Plug 7462900 Rotor 7397609 User s Manual 7970030 CentriVap DNA Concentrator 230V EU 1336100 Power Cord with CEE 7 7 Plug 7462900 Rotor 7397609 User s Manual 7970035 CentriVap DNA Concentrator 230V UK 1332600 Power Cord with BS 1363 Plug 7462900 Rotor 7397609 User s Manual 7970037 CentriVap DNA Concentrator 230V China 1332700 Power Cord with CHI 10P Plug 7462900 Rotor 7397609 User s Manual If you do not receive one or more of the components listed for your CentriVap or if any of the components are damaged contact Labconco Corporation immediately for further instructions 6 Product Service 1 800 522 7658 Chapter 3 Getting Started Setting Up Your CentriVap After you verify receipt of the proper components move your CentriVap to the level and sturdy location where you want to install it There is no need to secure the CentriVap DNA Concentrator to the work surface Then follow the steps listed below Emergency Access Into the Chamber The CentriVap is designed to prevent access to the chamber in the event of a power disruption If it is necessary to open the lid when there is no electrical power connected to the CentriVap insert a small screwdriver or similar instrument into the small round hole on the left side of the case near the top behind the control panel This will unlock the lid latch mechanism While holding the screwdriver in place raise the lid
3. Product Service 1 800 522 7658 25 Chapter 7 Troubleshooting Other corrective actions for potential problems are as follows PROBLEM CAUSE CORRECTIVE ACTION MOTOR ERROR Unit will not operate Excessive vibration Sample odor in lab Evaporation rate is reduced No vacuum poor vacuum Unit starts and shuts off Defective motor Hall effect sensor failure Wire failure Unit not connected to electrical power Circuit breaker blown Lid open Sample tubes not located symmetrically in rotor Vent hose exhausting into lab area Heater inoperable Vacuum pump failure Obstruction in hose Lack of adequate vacuum Pump not on Liquid in glass trap Control valve open Leaks in lines or connectors or gasket Foreign material on lid gasket Latch optical sensor improperly calibrated Replace motor Replace sensor Replace wire Connect unit to proper electrical receptacle Correct electrical problem and reset circuit breaker by pressing button Close lid Reposition sample tubes Redirect hose to fume hood Contact Labconco Check pump Remove obstruction or replace hose See below Turn on pump Empty trap Check control valve Locate and repair Clean gasket and lid Unplug power cord Wait 10 seconds Plug in power cord 26 Product Service 1 800 522 7658 Appendix CentriVap Components The following pages list components that are a
4. User s Manual CentriVap DNA Systems Models 7970010 7970011 7970030 7970035 7970037 To receive important product updates complete your product registration card online at register labconco com Protecting your laboratory environment ent 2 LABCONCO Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 2696 800 821 5525 816 333 8811 FAX 816 363 0130 E MAIL labconco labconco com HOME PAGE www labconco com Please read the User s Manual before operating equipment Copyright 2009 Labconco Corporation All rights reserved The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following Purifier amp Logic Biological Safety Cabinets and PuriCare Lab Animal Research Stations carry a three year warranty from date of installation or four years from date of shipment from Labconco whi
5. switch 9 Ifthe run time set point is used at the end of the set time an alarm sounds All functions cease Product Service 1 800 522 7658 15 Chapter 4 Using Your CentriVap 10 Press STOP to terminate operation if the CentriVap has not already stopped itself 11 When the evaporation is complete allow the rotor to stop moving lift the lid and remove the samples Operational Notes When the CentriVap is turned on the CentriVap returns to the same mode or STOP that it was in when the power was turned off If the CentriVap was in the RUN mode when the power was turned off when the power is turned on the CentriVap attempts to return to the programmed set points and continue the run To reset the timers to the original set point when running other than program 1 program 2 or program 3 press the start button and hold it for five seconds The display will indicate that the timer is reset Interrupting a Cycle After it Has Begun At any time during a run the cycle may be stopped by pressing the STOP button This shuts off all operating functions After the rotor stops the lid may be opened If it is necessary to re start the CentriVap close the lid and press RUN The CentriVap resumes operation at the same set point parameters and the timer continues to count down from the time at which the CentriVap was stopped Gas Ballast The vacuum pump incorporated in the CentriVap DNA Concentrat
6. following maintenance schedule is recommended Before servicing the CentriVap disconnect electrical power Special precautions must be observed if materials used in the CentriVap Concentrator are known to be hazardous toxic radioactive or contaminated with pathogenic micro organisms Before servicing the CentriVap Concentrator must be suitably decontaminated Wear appropriate eyewear gloves and other safety apparel As needed Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage equipment 1 Clean up all spills remove liquids from the chamber Clean or decontaminate all surfaces using agents suitable for the substance spilled 2 Clean lid and gasket using soft cloth sponge or chamois and a mild non abrasive soap or detergent 3 At the end of the run liquid collected in the glass traps should be removed empty a trap unscrew the glass bowl from the head Dispose of the liquid in accordance with all codes Reinstall the glass bowl by screwing it back on to the head 4 Ifthe media in the optional clear canister has changed color discard and replace the insert with a new insert For the radiochemical trap insert no indicator exists therefore it should be discarded after each use In radioactive applications the system should be monitored with a Geiger counter 5 Check rotors for loose or missi
7. 1 800 522 7658 Appendix CentriVap Dimensions LID SHOWN IN BOTH OPEN AND CLOSED POSITIONS INCH CENTIMETERS Product Service 1 800 522 7658 29 Appendix CentriVap Specifications This Appendix contains technical information about the CentriVap including specifications environmental operating conditions wiring diagrams and evaporation rates Electrical Specifications Nominal amperage for 115V CentriVap model 7970010 including vacuum pump 5A Nominal amperage for 230V CentriVap model 7970011 including vacuum pump 3A Frequency all models 50 60 Hz Phase Single Heater power 300 watts Rotor Speed Up to 1 725 RPM Environmental Conditions 30 Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient overvoltages according to Installation Categories Overvoltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 i e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be
8. Toluene Chapter 3 Getting Started When using compounds in the CentriVap that are hostile to the materials of construction it is imperative that the equipment is appropriately maintained e After each run clean up all residues spills and materials that might have splashed in the chamber using agents suitable for the substance involved e Ifthe compounds used attack acrylic consider using the optional glass chamber lid Contact Labconco for ordering information If optional secondary traps are used monitor their condition often and replace them when they are saturated A new acid trap is off white and changes color to purple when used up A new moisture trap is blue and changes color to pink when it is used up The solvent trap molecular sieve does not change color when saturated so extra care must be taken to determine when a replacement cartridge should be installed Solvent Safety Precautions CAUTION The CentriVap is not classified as explosion proof It has been designed with safety as a primary consideration and should be used in a prudent manner using good laboratory practices It has been designed for use with compounds as described in the United States National Electrical Code Class I Group D The heater may be programmed to run as hot as 100 C however the heater element may normally run at 110 C A thermal fuse limits the heater to a maximum temperature of 141 C It is important that the so
9. chamber during processing A timed outlet for the vacuum pump connection delays the start of the pump until the rotor reaches operating speed A safety switch prevents the concentrator from starting when the lid is in the open position and a latch prevents the lid from being opened while the rotor is turning The compact low profile design allows the concentrator to be placed on a bench top or other laboratory work surface All wetted parts within the pump are either Teflon Teflon coated or stainless steel A glass trap is provided to remove liquid from the system before it enters the pump and a second glass trap is provided on the outlet side of the pump to trap liquid expelled from the pump The microprocessor which controls the operation of the heater and the motor provides excellent regulation and reproducibility of protocols A manually preset timer can signal the end of the run Nine different protocols can be stored in memory so exact test parameters can be easily repeated Teflon is a registered trademark of DuPont Product Service 1 800 522 7658 1 Chapter 1 Introduction Safety Symbols Your CentriVap Concentrator was designed with safety in mind however conditions may exist that could be hazardous Throughout this manual potentially hazardous conditions are identified using the following words and symbols WARNING Read and understand operator s manual before using this machine Failure to follow o
10. pressing the increase or decrease buttons The set point is 100 C if the display shows HI The last entered set point is stored in memory Change Run Time or Heater Time Set Point To change the time set point press the set point SELECT button until arrows point to the Heater Time or Run Time set point which can be changed from 1 to 999 minutes by pressing the increase or decrease buttons If it is desired to have the CentriVap run continuously without alarming at the end of a time period press the increase button until the Run Time display says ON The last entered set point is stored in memory If during a run the STOP button is pressed the timers remember the time at which stop occurred If RUN is then pressed the timers continue to count down from the time at which they were stopped If you are running program 1 program 2 or program 3 pressing PROG 1 PROG 2 or PROG 3 resets the timers to the original set point time and the CentriVap starts a new run 14 Product Service 1 800 522 7658 Chapter 4 Using Your CentriVap To reset the timers to the original set point time when running programs other than program 1 program 2 or program 3 press the start button and hold it for five seconds The display will indicate that the timer is reset Operating the CentriVap CAUTION To avoid personnel injury Do not operate the CentriVap if the lid is scratched or ni
11. tubes or 36 13 x 75 mm tubes or 36 13 x 100 mm tubes and 24 16 x 100 mm tubes or 18 16 x 120 mm conical tubes or 18 17 x 95 mm tubes or 18 17 x 100 mm centrifuge tubes or 18 17 x 120 mm tubes 7455100 Rotor 12 13 mm Holds 40 1 5 ml microcentrifuge tubes or 7455101 40 2 0 ml microcentrifuge tubes and 16 12 x 55 mm tubes or 100 12 x 75 mm tubes or 64 12 x 95 mm tubes or 100 13 x 75 mm tubes or 64 13 x 100 mm tubes 7455200 Rotor 50 ml Holds 32 1 5 ml microcentrifuge tubes or 7455201 32 2 0 ml microcentrifuge tubes and 12 28 x 115 mm conical tubes or 12 28 x 135 mm conical tubes or 12 28 x 140 mm conical tubes 7461900 Rotor 96 well plate Holds 4 Standard 96 well plates or 7461901 2 Deep well 96 well plates 7460900 Clear Canister Accommodates inserts listed below 7814800 Acid Trap Insert 7814900 Moisture Trap Insert 7815000 Radiochemical Trap Insert 7815200 Solvent Trap Insert 7456600 Glass Lid for Concentrator Direct replacement for standard equipment acrylic lid For use with chemicals that could craze acrylic 7464300 CentriZap Strobe Light For observing samples while the rotor is spinning 7995600 Ammonia Trap Insert Teflon Coated Product Service 1 800 522 7658 21 Chapter 6 Accessories for Your CentriVap Installing a Glass Lid Turn off the CentriVap Unplug th
12. vented in a fume hood An accessory secondary trap is available to minimize the exhausting of solvents into the atmosphere This does not however negate the need to exhaust the vacuum pump into a fume hood Failure to properly vent the CentriVap will expose personnel to potentially harmful chemicals The CentriVap has not been evaluated by an approval agency for the use of biological radio toxins or flammable liquids or materials Solvents used in the CentriVap can cause skin eye respiratory and digestive system disorders Locate the CeniriVap inside a fume hood Space Requirements Refer to Appendix C CentriVap Specifications for dimensional drawings of the CentriVap No person or any hazardous material should be within 12 inches of the CentriVap Concentrator while it is operating 4 Product Service 1 800 522 7658 Chapter 3 Getting Started Now that the site for your CentriVap is properly prepared you are ready to unpack inspect install and test your CentriVap Read this chapter to learn how to e Unpack and move your CentriVap e Set up your CentriVap e Connect the electrical supply source to your CentriVap e Properly exhaust your CentriVap e Safely use solvents with your CentriVap CAUTION The CentriVap Concentrator weighs over 80 Ibs 36 Kg The carton allows for lifting with a mechanical lift truck or hand truck If you must lift the CentriVap manually use at least two 2 persons and follow safe li
13. with the other hand CAUTION Never attempt to defeat the latch or open the lid while the CentriVap is running Personnel injury can result from moving parts and chemicals CAUTION Moving rotor can cut hands Do not lift the lid while it is turning Do not disable the lid latch Do not reach into the chamber if rotor is turning Exhaust Port It the CentriVap is not located in a fume hood attach one end of a 50 inch ID hose to the exhaust port on the left side on the vacuum pump glass trap exhaust port Clamp securely Route the other end to a fume hood or other laboratory ventilation device Electrical Connection Plug the power cord into the receptacle on the back of the CentriVap Concentrator and plug the other end into a suitable power receptacle Rotor Installation Place the rotor onto the shaft of the Concentrator Rotate the rotor slightly to engage the drive pin in the shaft with the slots in the rotor hub The top of the shaft should be in line with the top of the rotor hub IMPORTANT Do not use a rotor if it shows any signs of damage Product Service 1 800 522 7658 7 COMPONENT Rotor Hub Lid Chamber Chamber Tubing Lid Gasket Rotor Rotor Shaft Bearings Chapter 3 Getting Started Chemical Resistance of CentriVap Components Your CentriVap DNA Centrifugal Concentrator is designed to be chemical resistant to most compounds that are commonly used in the concentration processes tha
14. 5 16 Closed 8 25 1 5 132 1 100 11 12 00 Ethanol 1 5 132 1 45 82 Closed 1 61 1 5 132 1 60 55 Closed 2 40 1 5 132 1 75 37 Open 3 57 1 5 132 1 100 22 Open 6 00 Product Service 1 800 522 7658 33 DECLARATION OF CONFORMITY Application Council Directive s 73 23 89 336 EEC 2002 95 EC RoHS 2004 108 EC Standard s to which conformity is declared EN61010 1 EN61326 1 EN61000 3 2 3 EN55022 Manufacturer s Name Labconco Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Laboratory Equipment Model No CentriVap DNA System 7970011 7970030 7970035 CentriVap Benchtop Concentrator 7810011 7810013 7810015 7810017 Acid Resistant 7310030 Refrigerated 7810030 7810031 7810032 7810033 7810034 7810035 7810036 7810037 CentriVap Refrigerated Concentrator 7310030 7310031 7310035 7310036 CentriVap Cold Trap 7811030 7811035 7460030 CentriVap Mobile Console 7812011 7812014 7812035 7812036 Serial No Various See Individual Declaration Year of Manufacture 2005 and Subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s See individual Declaration of Conformity which will be signed by the importer for your country Place Signature Date
15. HEAT button The display will show SP XXX ACT Press the increase or decrease button until the desired set point SP is displayed The actual chamber temperature ACT is displayed to the right When power is being supplied to the heaters a bar under the actual temperature will illuminate The chamber will continue to preheat until either the PREHEAT button is pressed again or the RUN button is pressed Select existing program Operating parameters can be stored in memory so protocols can be repeated Nine programs can be stored To select a program press the set point SELECT button until arrows point to the program number indicating that this set point can be run or altered To change the program number press the increase or decrease button until the desired program number is displayed When the program number is changed all its set points change also to indicate the last entered parameters for that program Store frequently run protocols in program 1 2 or 3 Then by pressing PROG 1 PROG 2 or PROG 3 the stored program will be initiated without having to press any other button Pressing just the one button starts the rotor the heater the timers and the vacuum pump Change Temperature Set Point To change the Temperature set point press the set point SELECT button until arrows point to the Temperature set point which can be changed from OFF up to 99 by
16. IM PARIET pO O2NO28V1 1099627009672 N d dVAINLN3D 02 0928 1 138v Qvd HOLIMS O 011 037108383 01003105 4907 011 3284005 NOYA 123NNOOSIQ ONINSVM Q0l Z N d SS3NHYH ASSY 011 DA M b 0005597 N d o SS3NBYH 123443 11VH dN3L 805835 dM3l lt moor 106 MJA LNOUS 553 437M YIMOd moot 140 15008 1Y3H N3d0 2 601 2 01 W101 511 009 3504 WWY3HL Nya 80i SAEN 3sv8 831V3H 15008 1V3H 131100 dMnd Mnnov 00 Av138 monavA 4 9 8v9i 00809 N d 5 NND 380315 p m 5 y EJ C 104100 380815 8 AYIYA WANIVA Product Service 1 800 522 7658 32 Appendix CentriVap Specifications Evaporation Rates Solvent Tube Size Numberof Sample Temp Time to Ballast Rate Overall ml Samples Size ml C Dry min ml min Acetonitrile 1 5 132 1 45 61 Closed 2 16 1 5 132 1 60 41 Closed 3 22 1 5 132 1 75 40 Closed 3 30 1 5 132 1 100 30 Closed 4 40 Methanol 1 5 132 1 35 68 1 94 1 5 132 1 45 60 Closed 2 20 1 5 132 1 60 45 Closed 2 93 1 5 132 1 75 34 Closed 3 88 1 5 132 1 100 23 Open 5 74 Water 1 5 132 1 45 440 Open 0 30 1 5 132 1 60 342 Open 0 39 1 5 132 1 75 195 Open 0 68 1 5 132 1 100 123 Closed 1 07 Methylene Chloride 1 5 132 1 45 20 Closed 6 60 1 5 132 1 60 18 Closed 7 33 1 5 132 1 7
17. Vap Specifications for complete CentriVap electrical and environmental conditions specifications and requirements Electrical Requirements The CentriVap Concentrator requires a dedicated grounded electrical outlet This outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V 60 Hz An 8 Amp circuit breaker or fuse is required for models rated at 230V 50 60 Hz Various models are available for use in different locations throughout the world Each has a unique plug suitable for the specific location See CentriVap Components in Chapter 3 for detail plug specifications If the plug supplied with the CentriVap Does not match with the available receptacle remove this plug and replace it with an approved plug of the suitable style It is recommended that an emergency switch for disconnecting the mains in the case of a malfunction be located remote from the CentriVap preferably outside the room in which the CentriVap is housed or adjacent to the exit from that room Product Service 1 800 522 7658 3 Chapter 2 Prerequisites Location and Exhaust Requirements The CentriVap Concentrator should be located on a surface that is stable flat and level WARNING The CentriVap System should be located within a fume hood if hazardous or flammable solvents are used Heating of materials could lead to the liberation of hazardous gases In all cases regardless of the solvent used it is strongly recommended that the vacuum pump is
18. assware Into the CentriVap Temperature Setting Guidelines Time Setting Guidelines CentriVap Controls Operating the CentriVap Controls Operating the CentriVap Operational Notes Interrupting a Cycle After it Has Begun Gas Ballast Safety Precautions CHAPTER 5 MAINTAINING YOUR CENTRIVAP Ne BBW 0 Oo CHAPTER 6 ACCESSORIES FOR YOUR CENTRIVAP Installing a Glass Lid Installing a Secondary Chemical Trap Installing a Vacuum Gauge Installing a CentriZap Strobe Light CHAPTER 7 TROUBLESHOOTING APPENDIX A CENTRIVAP COMPONENTS APPENDIX B CENTRIVAP DIMENSIONS APPENDIX C CENTRIVAP SPECIFICATIONS Electrical Specifications Environmental Conditions Wiring Diagram Concentrator 115V Wiring Diagram Concentrator 230V Evaporation Rates DECLARATION OF CONFORMITY 20 22 22 23 24 25 27 29 30 30 30 31 32 33 34 Chapter 1 Introduction Congratulations on your purchase of a Labconco CentriVap DNA Centrifugal Concentrator Models are available for operation on 115V or 230V The CentriVap Concentrator uses centrifugal force with heat and vacuum to rapidly dry precipitates of DNA and RNA samples Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial This allows recovery of solutes from sample volumes as small as a few microliters The concentrator is equipped with a 300 watt heater to speed evaporation by warming the
19. chever is sooner SteamScrubber amp FlaskScrubber Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco whichever is sooner Blood Drawing Chairs carry a ten year warranty Carts carry a lifetime warranty Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is not covered Returned or Damaged Goods Do not return goods without the prior authorization from Labconco Unauthorized returns will not be accepted If your shipment was damaged in transit you must file a claim directly with the freight carrier Labconco Corporation and its dealers are not responsible for shipping damages The United States Interstate Commerce Co
20. cked or shows signs of damage A damaged lid could fail under vacuum CAUTION Moving rotor can cut hands Do not lift the lid while it is turning Do not disable the lid latch Do not reach into the chamber if rotor is turning While the CentriVap Concentrator is operating do not lean on the lid do not stand near it longer than necessary and do not place hazardous materials within 12 inches Turn ON the CentriVap power switch Select a program or set the set point parameters Preheat the chamber if desired dec up cose Place samples in vials Normally the vials should be no more than half full Place vials in a rotor Load the rotor with samples into the chamber 6 Close the lid 7 Press RUN Ifthe display was showing set point parameters it will change to show actual parameters Press RUN again The S STOP in the display changes to RUN The program on the display alternately displays R or the number of the program that is running If you intend to run program 1 2 or 3 simply press PROG 1 PROG 2 or PROG 3 to quick start the CentriVap The rotor will start the vacuum break valve will close and the vacuum pump will start after the rotor reaches operating speed 8 Set point parameters can be altered at any time during a run by first selecting the parameter using the set point SELECT button and then pressing the INCREASE or DECREASE
21. e dumping of waste materials in or upon water land or air and to comply with such regulations 10 Product Service 1 800 522 7658 Chapter 4 Using Your CentriVap After your CentriVap has been installed as detailed in Chapter 3 Getting Started you are ready to begin using your CentriVap Read this chapter to learn how to e Set operating parameters Operate the controls e Properly select and position glassware inside your CentriVap e Understand the display Interrupt a cycle after it has begun NOTE See Appendix C CentriVap Specifications for electrical requirements NOTE Do not store or stack supplies or equipment on top of the CentriVap Product Service 1 800 522 7658 11 Chapter 4 Using Your CentriVap Planning Thoroughly understand procedures and the equipment operation prior to beginning work The unique performance of the CentriVap is dependent upon the proper balance of heat vacuum and centrifugal force If the proper balance is not established it is possible to damage or lose a portion of the sample Therefore if you are unfamiliar with the CentriVap or are attempting a new protocol it may be helpful to make a trial run that is void of the sample you are attempting to concentrate Glassware Selection Normally sample tubes should be filled no more than approximately half full Select the size of the sample tube so it is compatible with the rotor and the desired sample size Tubes should not be loos
22. e in the rotor Rotors are available with holes for various size tubes Refer to Chapter 6 Accessories for your CentriVap DNA Concentrator for available rotor sizes Loading Glassware into the CentriVap Smooth operation of the CentriVap is dependent upon proper balance of the machine Therefore if less than a full load of samples is run it is important to load samples into the CentriVap in a fairly symmetrical manner distributing the weight of the samples evenly in the sample rotor Temperature Setting Guidelines The evaporation rate achieved by the CentriVap is dependent upon a variety of factors These include the nature of the solvent the temperature and the pressure in the vacuum system As a general guideline to speed the evaporation process the CentriVap temperature should be set as high as possible as long as the temperature will not damage the sample or cause the sample to bump Time Setting Guidelines The CentriVap Concentrator has two timers The RUN TIME turns the entire concentrator off after the user set period of time This stops the rotation turns off the heater and vacuum pump and bleeds vacuum from the system The heater may be turned off prior to the concentrator turning off so heat sensitive samples may be protected from exposure to excessive heat after the solvent has evaporated To turn the heater off prior to stopping the entire system enter a set time into the memory at the HEATER TIME When the pr
23. e power cord from the wall receptacle Pull outward on the hinge pin knob Lift and remove the old lid To install the new lid reverse the process Installing a Secondary Chemical Trap An accessory secondary chemical trap is available to minimize the exhausting of solvents into the atmosphere Attach the bracket to the left hand side of the housing using the screws provided Attach the hose from the outlet on the vacuum pump to the out connector of the canister housing Unscrew the clear bowl of the canister housing from the head Remove both the upper and lower caps from the filter cartridge and insert the small end of the cartridge into the hole in the center of the head Reinstall the clear bowl LUEEEELOE 7 CLEAR CANISTER 22 Product Service 1 800 522 7658 Chapter 6 Accessories for Your CentriVap Be sure to use the proper cartridge for your application The moisture cartridge is used to trap water vapor The acid cartridge is used to trap acid vapors The solvent cartridge is used to trap solvent vapors and the radiochemical cartridge is used to trap radioactive waste When the media in the insert has changed color discard the insert and replace it with a new insert For the radiochemical trap insert no color indicator exists therefore it should be discarded after each use Use a Geiger counter to monitor the pump exhau
24. expected in accordance with IEC 664 Product Service 1 800 522 7658 5 t iVap Speci Centr endix C A Diagram 115V Model iring W Y AJY 005 6 N d 09 06 ASIL 18 dVAIIN32 WVYOVIC INIXIM OONOO28V 1 AZI NY 4 E lo NO NY4 N3dO QN 032194383 1NO3N3 010 3105 3201 Qn 204 lt 06 N d 91 021 1 2 02 028 1 138 1 HOLIMS HOLIMS 011 39unos u3MOd WONJ 123NNOOSIQ nO o ON ONINYVM P n NI lt 775 812 5 YOSN3S 1 2190 3sv8 M A79 viz lo 0042 N d m SS3NUWH ASSY 011 DA 3sv8 CA AINO 32N383338 N03 VOd 310N 318 131 00098 v7 N d udi SS3NHVH 123443 11VH dN31 G LHM 942 MOSN3S ANIL 4 lt moot 338NYH2 H31V3H NIV 00 45 lt N d 0 SS3NHYH avaa 038 8v6 NBB BYOI 338 581 MOOT UJ 00 N3402 60 250 5 Om W101 S11VM 009 Isna Waa 8 8 980 879 55 88 o z no WANIVA NYB VOI o AVT38 131 ANNIYA Av138 N31V3H 15008 1v3H uo1I0Ydv2 008092 S E Nn9 380B1S B b E S C V HE o JAIA 1 91 0 380515 3331238 038 WnnovA 31 Product Service 1 800 522 7658 CentriVap Specifications endix C A Wiring Diagram 230V Model A38 LOSL6EL N d AZI NY4 09 05 AOEZ 18 dYAIMIN32 WVYOVIC 9NIM
25. fting guidelines CAUTION Lifting hazard Do not lift or move this equipment without assistance Product Service 1 800 522 7658 5 Chapter 3 Getting Started Unpacking Your CentriVap Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit If your CentriVap is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery NOTE Do not return goods without the prior authorization of Labconco Unauthorized returns will not be accepted If your CentriVap was damaged in transit you must file a claim directly with the freight carrier Labconco Corporation and its dealers are not responsible for shipping damage Do not discard the carton or packing material for your CentriVap until you have checked all of the components and installed and tested the CentriVap CentriVap Components As previously mentioned the CentriVap DNA System is available in 115V or 230V Locate the model of CentriVap you received in the following table Verify that the components listed are present and undamaged Catalog Product Description 7970010 CentriVap DNA Concentrator 115V USA 1334500 Power Cord with NEMA 5 15P Plug 7462900 Rotor 7397609 User s Manual 7970011 CentriVap DNA Concentrator 230V
26. lvents used are compatible with these temperatures Do not evaporate solvents that have an autoignition temperature below 180 C Do not evaporate solvents that are classified as Group A B or C by the National Electrical Code Evaporate only non flammable or Group D solvents with autoignition temperatures 180 C or above Use of other compounds could cause an explosion Solvents may be flammable Evaporate only non flammable or group D solvents with autoignition temperature 180 C or higher Keep sources of ignition away from solvents Product Service 1 800 522 7658 9 Chapter 3 Getting Started CAUTION Solvents used in the CentriVap may be flammable or hazardous Use extreme caution and keep sources of ignition away from the solvents When using flammable or hazardous solvents both the CentriVap and the vacuum pump should be operated inside a fume hood If a sample is spilled in the chamber it must immediately be cleaned up Hazardous materials such as strong acids or bases radioactive substances and volatile organics must be handled carefully and promptly cleaned up if spilled Do not store flammable or hazardous solvents within 12 inches 300 mm of the CentriVap IMPORTANT The disposal of substances used in connection with this equipment may be governed by various Federal State or local regulations All users of this equipment are urged to become familiar with any regulations that apply in the user s area concerning th
27. mmission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical assistance contact Labconco s Customer Service Department or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Part 7397609 Rev ECO F572 TABLE Or CONTENTS CHAPTER 1 INTRODUCTION Safety Symbols CHAPTER 2 PREREQUISITES Electrical Requirements Location and Exhaust Requirements Space Requirements CHAPTER 3 GETTING STARTED Unpacking Your CentriVap CentriVap Components Setting Up Your CentriVap Emergency Access Into the Chamber Exhaust Port Electrical Connection Rotor Installation Chemical Resistance of CentriVap Components Solvent Safety Precautions CHAPTER 4 USING YOUR CENTRIVAP Planning Glassware Selection Loading Gl
28. ng parts Tighten or replace as required Do not use defective rotors 6 Inspect the chamber to insure that there are no cracks or structural damage Call Labconco if defects exist 7 Check the continuity of the protective earth between the ground terminal on the power inlet and a bare metal housing panel Contact Labconco if there is no continuity 8 Repair any defects to the surface where the CentriVap is installed 18 Product Service 1 800 522 7658 Chapter 6 Accessories for Your CentriVap Daily 1 To prolong the life of the DNA vacuum pump and to optimize its performance it is important to purge all liquid from within it When the DNA concentration process is completed open the CentriVap lid and push the rotor switch ON In ten seconds the pump will operate and will draw air through the system The rotor will not turn with the lid open The length of the liquid purge time is dependent on the volatility of the solvents used Use of water may require an overnight purge time Monthly 1 The rubber components the CentriVap may eventually deteriorate and require replacement The effective life of rubber parts depends upon both their usage and the surrounding environment Check all rubber hoses and gaskets and replace any that show signs of hardening permanent set or deterioration Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the glass lid Using a soft cloth sponge o
29. ogrammed time expires the heater will turn off but the rotation and vacuum will continue until the run time expires 12 Product Service 1 800 522 7658 Chapter 4 Using Your CentriVap The tables in Appendix C CentriVap Specifications indicate approximate times required to evaporate various common solvents Actual times must be determined by the user The CentriVap can be set to alarm after a preset period of operation When the time expires the CentriVap will give an audible alarm and turn itself off CentriVap Controls The control panel for the CentriVap is shown below with a description about its function 1 Display The liquid crystal display LCD shows set point parameters and actual measured conditions 2 Program Buttons Used to initiate the start of a run with the use of just one button Run Stop Button Used to start or stop a run 4 Preheat Button Used to turn on the heater to preheat the chamber prior to loading samples 5 Increase Button When pressed the last selected set point will increase Decrease Button When pressed the last selected set point will decrease 7 Set Point Select Button To select a parameter to change press the select button Arrows on the display will point to the parameter that may be altered 95 e Product Service 1 800 522 7658 13 Chapter 4 Using Your CentriVap Operating the CentriVap Controls Preheat To preheat the chamber press the PRE
30. or is capable of achieving an ultimate pressure of lt 2 MBAR Some solvents such as water may freeze when exposed to this very low pressure If the sample being processed freezes evaporation ceases and the sublimation process occurs which in turn greatly reduces the concentration rate Freezing of the sample may be prevented by opening the gas ballast valve which increases the ultimate pressure in the system Pressure may be monitored using a vacuum gauge as described in Chapter 6 Installing a Vacuum Gauge 16 Product Service 1 800 522 7658 Chapter 4 Using Your CentriVa GAS BALLAST VALVE Safety Precautions Special precautions must be observed if the materials used in the CentriVap Concentrator are known to be hazardous toxic radioactive or contaminated with pathogenic micro organisms These actions should include but are not limited to the following e Refer to the World Health Organization Laboratory Biosafety Manual paying special attention to information about centrifuges and the handling of hazardous materials Operate or vent the CentriVap Concentrator inside a suitable fume hood or ventilation device Load rotors in a ventilation device e Periodically inspect all parts of the CentriVap Concentrator including the lid gasket chamber plumbing components and rotors Product Service 1 800 522 7658 17 Chapter 5 Maintaining Your CentriVap Under normal operation the CentriVap requires little maintenance The
31. perating instructions could result in death or serious injury This is the safety alert symbol It is used to alert you to potential personal injury hazards Obey all safety messages that follow this symbol to avoid possible injury or death DANGER WARNING CAUTION CAUTION It is important that you understand the warnings listed throughout this manual DANGER indicates an imminently hazardous situation which if not avoided will result in death or serious injury WARNING indicates a potentially hazardous situation which if not avoided could result in death or serious injury CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury CAUTION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property damage before you operate the CentriVap Concentrator Product Service 1 800 522 7658 Chapter 2 Prerequisites Before you install your CentriVap you need to prepare your site for installation Carefully examine the location where you intend to install your CentriVap You must be certain that the area is level and of solid construction In addition an exhaust means must be provided An electrical source must be located near the installation site Carefully read this chapter to learn e The electrical supply requirements e The exhaust requirements Refer to Appendix C Centri
32. ptacle on the back of the CentriVap marked STROBE OUTLET Peel the protective backing off the enclosed Velcro and attach it to the back of the vacuum pump housing Secure the power supply box on the harness to the Velcro Place the light in the holder To use the strobe light while the CentriVap is operating remove the light from its holder press the trigger and shine the light on the samples in the rotor STROBE RECEPTACLE BOX SECURED WITH VELCRO 24 Product Service 1 800 522 7658 Chapter 7 Troubleshooting Refer to the following if your CentriVap fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance The following failure codes may appear on the display when problems are sensed by the internal self check routine CAUTION Disconnect power before corrective action is taken AX CAUTION Electrical shock hazard See appendix C CentriVap specifications for electrical operating parameters DISPLAY ERROR CODE CAUSE Heat Sensor Sensor failure Connection failure Close Lid Lid open Latch Fail Solenoid failure Switch or sensor failure Mem Fail P1 Memory failure New memory IC chip Bad memory IC chip CORRECTIVE ACTION Replace sensor assembly Repair connection Close lid Check component Check connections Push program button 71 Push program button 1 Call Labconco Replace IC chip or control PCB
33. r chamois and a mild non abrasive soap or detergent clean the exterior surfaces of the unit Liquid spray cleaners and polishes may be used on the exterior surfaces Do not use solvents to remove stains from the exterior surfaces as they may damage the finish Product Service 1 800 522 7658 19 Chapter 6 Accessories for Your CentriVap The configuration of your CentriVap can be changed to accommodate your needs If the solvents used in the CentriVap degrade the acrylic lid it may be replaced with an optional glass lid to gain added chemical resistance You may wish to add a secondary trap to trap vapors exhausted from the vacuum pump To observe the samples while they are processing an optional CentriZap Strobe light may be installed Read this chapter to learn how to e Install a glass lid e Install a chemical trap e Install an accessory vacuum gauge e Install a CentriZap strobe 20 Product Service 1 800 522 7658 Chapter 6 Accessories for Your CentriVap The following accessories are available for the CentriVap DNA Concentrator PART DESCRIPTION 7462900 Rotor DNA Holds 72 0 5 ml microcentrifuge tubes 7462901 and 60 1 5 ml microcentrifuge tubes or 60 2 0 ml microcentrifuge tubes 7450700 Rotor 1 5 ml Holds 132 1 5 ml microcentrifuge tubes or 7450701 132 2 0 ml microcentrifuge tubes 7455000 Rotor 15 ml Holds 44 12 x 55 mm tubes or 7455001 36 12 x 75 mm tubes or 36 12 x 95 mm
34. st Warning This radiochemical cartridge does not meet NRC filter design recommendations After operating properly dispose of all hazardous materials in compliance with all applicable codes Labconco is not responsible for improper disposal of any materials Installing a Vacuum Gauge A user supplied vacuum gauge may be attached to the CentriVap to monitor the vacuum level Attach the vacuum gauge to the barb end of the Quick Disconnect Coupling fitting that was supplied with the CentriVap using a length of suitable rubber hose The Quick Disconnect Coupling fitting can then be pushed into the mating connector on the left side of the CentriVap located towards the rear The Quick Disconnect Coupling fitting can be removed from the mating connector by pressing on the tab on the connector and then pulling out the Quick Disconnect Coupling fitting Always remove the Quick Disconnect Coupling from the CentriVap when a vacuum gauge is not attached to it PRESS TAB TO RELEASE COUPLING QUICK DISCONNECT COUPLING VACUUM TUBING USER SUPPLIED VACUUM GAUGE Product Service 1 800 522 7658 23 Chapter 6 Accessories for Your CentriVap Installing a CentriZap Strobe Light An accessory strobe light is available to enable you to see the samples as they are rotating in the rotor Attach the holder to the right hand side of the CentriVap Concentrator using the screws provided Plug the connector on the strobe light harness into the rece
35. t are performed in it However by necessity the CentriVap is comprised of a number of different materials some of which may be attacked and degraded by certain chemicals The degree of degradation is obviously dependent on the concentration and duration of exposure Some major components of the CentriVap that are susceptible to degradation are as follows Acids Bases Solvents i 9 E E E 2 A a 5 x 5 5 aS e els 5 S E eE Ex 5 8 519 5 5 2 _ 6 2 8 5 5 2 4 9 2 5 12 ela lt 4151554108 5 2 2 3 2 2 35 2 2 S gli g 8 o 5 5 E 515 5 9 lt S 2 Z Z E s So 5 Eleg x ele 5 lal 51 5 E olol 5 51 ojojo Delrin C C D D D ID Acrylic EE Jclib c Jpbfbf Epoxy coated C C Aluminum id ef fet j fe telef LETTE EEE LLLI LLL fed fel Anodized Aluminum Stainless Steel Di J High Carbon pip p pipipip D Steel C Moderate Degradation Questionable use D Severe Degradation Infrequent use recommended immediate thorough cleaning required e Diaphragm vacuum pumps sold by Labconco have wetted parts either made from Teflon or protected by Teflon coatings and are suitable for nearly all procedures 8 Product Service 1 800 522 7658 O O
36. vailable for your CentriVap The parts shown are the most common replacement parts If other parts are required contact Product Service Item Quantity Part No Description 1 1 7459000 Motor w Capacitor 2 1 7481700 Valve Vacuum Break 115V 2A 1 7481701 Valve Vacuum Break 230V 3 1 7453500 Heater 115V 3A 1 7453501 Heater 230V 4 1 1647106 O Ring Outer 5 1 1647105 O Ring Inner 6 1 7975902 Fuse High Temp Limiter 7 1 7456000 Harness Temp amp Speed Sensor 8 1 7479400 PCB 9 1 1289200 Relay Vacuum Pump 115V 9A 1 1289100 Relay Vacuum Pump 230V 10 1 7826000 Switch Latch 11 1 7478700 Solenoid Latch 12 1 7403701 Display 13 1 7398400 Label Switch Pad 14 1 7452600 Gasket Lid 15 1 7452500 Lid Acrylic 16 42 2 inch 7451700 Extrusion 17 2 7452200 Hinge Bracket Assembly 18 1 1289300 Circuit Breaker 115V 18A 2 1289308 Circuit Breaker 230V 19 1 7356500 Vacuum Pump 115V 19A 1 7356501 Vacuum Pump 230V 20 2 7450200 Jar Trap 20A 2 7450300 Gasket Jar Trap Not Shown 1 1334500 Power Cord USA 115V Not Shown 1 1338000 Power Cord USA 230V Not Shown 1 1336100 Power Cord EU 230V Not Shown 1 1332600 Power Cord UK 230V Not Shown 1 1332700 Power Cord China 230V 21 1 1302300 Switch 22 1 7397601 Coupling Insert 23 1 7441500 Fan Product Service 1 800 522 7658 27 Appendix CentriVap Components 28 Product Service
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