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Multimode Detection Software User`s Manual

1. 5 22 Defining Editing and Running Protocols 4 Select the orientation that matches how the plate is positioned on the plate carrier Beckman Coulter Inc Note Landscape the long edges of the plate run parallel to the front of the instrument with well A1 located in the upper left corner Portrait the short edges of the plate run parallel to the front of the instrument with well A1 located in the upper right corner Opposite Landscape the edges of the plate run parallel to the front of the instrument with well A1 located in the lower right corner Opposite Portrait the short edges of the plate run parallel to the front of the instrument with well A1 located in the lower left corner Well A1 is identified in the plate graphic by a red highlight When preparing to run a fluorescence top fluorescence polarization or time resolved TRF protocol choose Optimize Read Height to automatically determine and set the optimal read height used in the protocol run refer to Section 5 3 1 1 Optimizing Read Height 880 only gt DTX 880 Optimize Read Height is available for this instrument only OR Select the read height manually e Default uses the read height defined in Labware Information for the labware type used in the protocol refer to Section 4 2 1 Defining Labware Information e Maximum sets the read height at 10 6 mm the maximum height supported by the instru
2. 6 3 1 1 6 10 Viewing Measurement Results Viewing Information About the Protocol General the first worksheet in all Excel files exported by Multimode Detection Software contains information about parameters configured in the protocol and the protocol run itself Figure 6 7 Fd Microsoft Excel Basic Endpoint Measurement Fluorescein 96 _Fluorescein Top 0_04 23 2004_01 26 06 40 xnml File Edit view Insert Osean amp J29 M Lav f Format Tools Data Window Help Be S n oa A 4 3 iw o B Je Operator Header Identification Date Description PlateName DateMeasured DateEvaluated ValidState Software Version Instrument Name Serial Number FW Version PIC Version Labware Name Rows Columns ProtectionState WellShape BottomShape Created DateEdited Operator Fluorescein 96 Fluorescein 96 4 23 2004 13 26 Description PlateNamelsDetined 4 23 2004 12 06 4 7 2004 9 06 Defined Apex 1 0 0 13 Multimode Reader 0 Standard 96 8 12 ReadOnly Round Flat 12 3 2003 18 00 42 3 2003 18 00 Figure 6 7 Viewing protocol information in the General worksheet excerpt To view the General worksheet In Excel choose the General tab in the lower left corner of the window Beckman Coulter Inc Viewing Measurement Results 6 11 6 3 1 2 Viewing Measurement Results Measurement results exported to Excel display raw data in matrices that represent the layout of
3. Editing Labware Creating and Editing Labware 4 11 4 5 Deleting Labware User defined labware not used in any measurement protocols may be deleted from the Labware Selection List Default labware included with the software installation and labware used in protocols may not be deleted To delete labware 1 In the Labware Selection List select the labware to delete 2 From the toolbar choose Delete the selected labware OR From the Actions menu choose Delete the selected labware OR Right click on the selected labware and choose Delete the selected labware from the menu that appears Message appears Figure 4 6 Message lt 4re you sure you would like to delete the labware Microplate96 e Figure 4 6 Confirming a labware deletion 3 Choose Yes to delete the selected labware OR Choose No to cancel the deletion Multimode Detection Software User s Manual 4 12 Creating and Editing Labware 4 6 Optimizing Labware Microplate dimensions may vary slightly between production lots which potentially affects measurement accuracy Multimode Detection Software provides the ability to create different labware lots for each type of labware which increases accuracy by optimizing labware dimensions and information for each production lot used Labware is optimized in Optimizing Labware Figure 4 7 which guides the process with a wizard type interface Optimizing labware includes
4. In Delay Before Measure seconds enter the interval in seconds between switching off the light source and performing the measurement The delay may be set within the range of 0 000001 to 0 0075 of a second In Integration Time seconds enter the length of time in seconds each sample is measured The integration time may be set within the range of 0 00005 to 0 0075 of a second Choose Save to save the new time resolved fluorescence detection method The new method appears in the Detection Method Selection List OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without saving the new detection method Creating and Editing Detection Methods 3 13 3 3 Editing Detection Methods Parameters configured in saved detection methods may be edited however the method technique may not be changed Default detection methods provided with the software and methods used in protocols may not be renamed To edit a detection method 1 In the Detection Method Selection List select the detection method to edit 2 From the toolbar choose Edit the selected method oR From the Actions menu choose Edit the selected method OR Right click on the selected detection method and choose Edit the selected method from the menu The Method Editor appears Figure 3 10 ay Method Editor Fluorescein Top Method Para
5. Multimode Detection Software User s Manual Beckman Coulter PN 987958 Revision AA May 2004 Beckman Coulter Inc 4300 N Harbor Boulevard Fullerton CA 92834 3100 Copyright 2004 Beckman Coulter Inc Printed in Austria Except as provided in writing signed by an officer to Beckman Coulter Inc this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but Beckman Coulter Inc does not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability currentness omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user Except as expressly provided herein Beckman Coulter Inc makes no other warranty whether oral or written expressed or implied as to any matter whatsoever including but not limited to those concerning merchantability and fitness for a particular purpose nor is freedom from any patent owned by Beckman Coulter Inc or by others to be inferred LIMITATIONS OF LIABILITY Beckman Coulter Inc shall not be liable to any extent whatsoever for any damages resulting from or arising out of the use or performance of this system and related documentation or the procedures s
6. setting up 1 11 system requirements 1 5 using the software interface 1 3 O online help accessing 1 4 optimizing labware 4 12 optimizing read height 5 23 R read height optimizing 5 23 preparation methods setting before a protocol run 5 22 configuring in protocols 5 8 configuring set temperature 5 16 S configuring shake 5 15 shaking printing configuring a shake preparation method 5 15 configuring settings 1 17 measurement results 6 13 protocols 5 31 protocols configuring an external program 5 20 configuring detection methods 5 8 configuring general settings 5 3 configuring labware layout 5 6 configuring methods in basic mode 5 9 configuring output settings 5 18 5 19 copying 5 29 creating 5 3 deleting 5 30 optimizing read height 5 23 printing 5 31 running in simulation mode 5 26 on an instrument 5 21 selecting labware 5 5 configuring in protocols 5 10 manual control 2 12 simulated data files choosing default 1 18 selecting before a protocol run 5 26 simulation mode choosing default simulated data files 1 18 running protocols 5 26 software settings choosing data storage directory 1 19 choosing default simulated data files 1 18 configuring 1 15 configuring print settings 1 17 setting system language 1 16 T temperature control configuring a set temperature preparation method 5 16 configuring in Instrument Settings 2 11 time resolved fluorescence detection methods configuring 3 11 Multimode Detection S
7. 0 62 1 458 1569 900 8514 366 8905 643 9944 27 97318 768 5553 286 7331 779 607 seconds 2 73736 250007 411448 661601 3011252 3559676 1170803 2629258 2 67459 193641 3212196 3992894 2840880 3650614 1205041 431113 2 276 1445 351 1539 657 5561 578 2213 261 8923 222 6142 220 9037 792 4225 1315337 325008 923796 2861616 900184 1717639 782263 3445672 3 1519509 2174919 1882346 1818640 3628996 1042925 3930877 126188 3 165 3383 611 5731 116 3443 434 6914 428 4839 453 0081 514 0328 955 5967 4 3443220 1107335 24667 1635649 61070 1702857 2448136 1121300 4 98854 1441045 1822134 2224397 2158282 771704 3304224 3119764 4 365 0225 106 9017 957 264 85 08855 912 7076 561 3134 88 84565 266 0577 5 158672 4984 3686759 136983 2740933 3045573 2412549 3859774 5 1370032 868017 347143 3350656 1669376 3422259 643694 2748100 5 685 2033 981 6479 262 7933 876 2792 451 7861 178 7702 716 1156 387 5105 Overflow Error Incorrect 1566808 3009800 2058571 458374 648881 2449378 3232367 1423468 6 2889290 3546105 1146330 1534553 28500 228784 138517 3726412 6 66 86736 155 7517 486 7109 349 7304 946 9808 890 5181 948 2374 237 532 7 1271043 2478041 1269501 1763470 1220745 2807387 69319 3872121 7 3848080 3097786 3249579 1895139 31772 2794769 410455 497815 304 8432 126 72
8. ABS 260 Absorbance Monochromatic 1 28 2004 1 28 2004 Select Center of Left Bottom Well Select Center of Right Top Well Absorbance Monochromatic 1 28 2004 1 28 2004 Absorbance Monochromatic 1 28 2004 1 28 2004 Select Center of Right Bottom Well Absorbance Monochromatic 1 28 2004 1 28 2004 Verify Well Centers Absorbance Monochromatic 1 28 2004 1 28 2004 Absorbance Monochromatic 1 28 2004 1 28 2004 Absorbance Monochromatic 2 27 2004 2 27 2004 Abs620Bichromatic Absorbance Bichromatic 3 1 2004 3 1 2004 Cancel Figure 4 7 Selecting the detection method for labware optimization To select the detection method 1 Choose the detection method that most closely matches the method used in the desired protocol For absorbance and fluorescence intensity top and bottom measurements this is the exact method used For luminescence fluorescence polarization and time resolved fluorescence measurements choose a method performed at the same or similar wavelength 2 Choose Next to Prepare Labware refer to Section 4 6 2 Preparing and Loading the Labware OR Choose Cancel to close Optimizing Labware without performing the optimization Multimode Detection Software User s Manual 4 414 Creating and Editing Labware 4 6 2 Preparing and Loading the Labware To optimize labware dimensions the four corner wells of the plate are read while empty Prepare Labware provides controls to load and eject labware from the ins
9. Beckman Coulter Inc Creating and Editing Detection Methods 3 17 3 6 Exporting and Importing Detection Methods User defined detection methods saved in the Detection Method Selection List can be exported to an XML file and imported later to restore that configuration or share it with a copy of Multimode Detection Software installed on another computer Exporting default detection methods included with the software installation is not necessary Since a default detection method may not be edited deleted or overwritten it is available to users at all times Because of this importing a default detection method from an XML export file is not permitted by the software To export a detection method 1 In the Detection Method Selection List select the detection method to export 2 From the File menu choose Export gt Detection Method Browse for Folder appears Figure 3 12 My Documents 4 This Computer amp My Network Places gj Recycle Bin Exported Protocols Make New Folder Figure 3 12 Choosing the folder where an exported detection method will be saved 3 In Browse for Folder browse to the folder where the exported detection method will be saved OR Choose Make New Folder to create a new folder where the exported detection method will be saved 4 Choose OK to export the detection method The exported detection method is saved using the default file name format Method__ MethodName xml
10. CY Perform after completing measurement s E create XML and dat data files Fi le format options V Perform after completing measurement s P Print options M Perform after completing measurement s Protocol Information V Labware Information Print options C General Information C Method Information Execute program Run protocol Run this protocol now Run this protocol now before saving gt Execute a program after protocol executes Cancel Figure 5 14 Configuring output settings To configure Output Settings 1 Choose Excel data file and print output options as desired Table 5 1 describes the options available 2 Choose Run this protocol now before saving to run the protocol immediately Refer to Section 5 3 Running Protocols for more information about running protocols 3 Choose Execute a program after protocol executes to display options for configuring an external software application to run immediately after the protocol run is completed Refer to Section 5 2 6 1 Configuring a Program to Run after Protocol Executes for more information 4 Choose Save to save the protocol and close Create Protocol OR Choose Cancel to close Create Protocol without saving the new protocol OR Choose Back to configure methods used in the protocol refer to Section 5 2 5 Configuring Detection and Preparation Meth
11. In order to import the file at a later date the filename must not be changed OR Choose Cancel to stop the operation without exporting the detection method Multimode Detection Software User s Manual 3 18 Creating and Editing Detection Methods To import a detection method from an exported XML file 1 From the File menu choose Import gt Detection Method Open appears 2 In Open browse to and select the desired XML file to import 3 Choose Open The detection method is imported to the Detection Method Selection List Beckman Coulter Inc 4 1 Overview Creating and Editing Labware Multimode Detection Software supports a wide range of labware with many common microplate formats already preconfigured and ready for use in protocols Configured labware types are listed in the Labware Selection List and are available for use in protocols New labware types may be created at any time using the Labware Editor The Labware Editor also provides the ability to edit and delete existing labware types not used in protocols make copies of labware types and optimize labware dimensions to compensate for slight dimensional variations that may exist between production lots gt DTX 800 supports 96 well and 384 well microplates gt DTX 880 supports 6 to 384 well microplates All labware functions are accessed from the Labware Selection List Figure 4 1 Labware functions include Creating Labware Secti
12. Vv Check if plate is inserted before each read Shake Control nee Liner x Ee EnA A SA Conta Intensity Low X Eject Load Duration s f5 Emission Filter Slide Control Shake Eject Load OK Cancel Apply Figure 2 5 Instrument Settings Manual Control 2 3 3 1 Temperature Control 880 only Temperature Control is used to set the microplate chamber temperature The temperature is set by heating the microplate chamber cooling the chamber is not supported Depending on the measurement wavelength and light source used in the protocol the temperature may range from 3 C 5 4 F or 4 C 7 2 F above ambient to 45 C 113 F Actual displays the current temperature inside the instrument To set the temperature 1 In Set Point enter the desired temperature in Celsius 2 Choose Set Temperature control is activated for the instrument and begins to heat to the desired temperature The set temperature is maintained until it is changed or the instrument is powered off Note It takes a minimum of 30 minutes for the instrument to reach the desired temperature The actual time required depends on the relative change in temperature To turn off temperature control In Set Point enter 0 OR Turn power to the instrument off and on Multimode Detection Software User s Manual 2 12 Setting Up and Controlling Instruments 2 3 3 2 Shake Control Shake Control is used to manually shake a 96 or 384 w
13. edited and tested for the selected instrument even though it is not physically connected to the host computer Beckman Coulter Inc Setting Up and Controlling Instruments 2 3 2 2 Controlling Instrument Functions amp Many common instrument functions such as ejecting and loading the plate carrier and initializing the instrument can be performed directly from the Instrument Selection List using the buttons on the toolbar Controlling instrument functions includes e Ejecting the Plate Carrier Section 2 2 1 e Loading the Plate Carrier Section 2 2 2 Initializing the Instrument Section 2 2 3 Connecting to the Instrument Section 2 2 4 e Enabling Simulation Mode Section 2 2 5 2 2 1 Ejecting the Plate Carrier Eject the plate carrier moves the plate carrier outside the instrument to allow access for placement or removal of a microplate To eject the plate carrier 1 In the Instrument Selection List select the desired instrument Figure 2 1 2 From the toolbar choose Eject the plate carrier OR From the Actions menu choose Eject the plate carrier OR Right click on the desired instrument and choose Eject the plate carrier from the menu that appears 2 2 2 Loading the Plate Carrier Load the plate carrier retracts the plate carrier and microplate back inside the instrument in preparation of performing a measurement To load the plate carrier 1 In the Instrument Se
14. it automatically connects to the instrument or starts in simulation mode if no instrument is detected The connection to the instrument may be established manually after physically connecting a different instrument to the computer or when switching from simulation mode refer to Section 2 2 5 Enabling Simulation Mode To connect to the instrument 1 In the Instrument Selection List select the desired instrument Figure 2 1 I 2 From the toolbar choose Connect to the instrument The button remains depressed while the instrument is connected and not in simulation mode OR ol i Beckman Coulter Inc From the Actions menu choose Connect to the instrument OR Right click on the desired instrument and choose Connect to the instrument from the menu that appears Setting Up and Controlling Instruments 2 5 2 2 5 Enabling Simulation Mode Multimode Detection Software can operate in simulation mode whether or not an instrument is connected Simulation mode enables all features supported by the instrument currently selected in the Instrument Selection List but measurement results are randomly generated by the software or read from a file Refer to Section 1 4 2 3 Choosing Simulated Data Files for more information about selecting simulated data files To enable simulation mode 1 In the Instrument Selection List choose the desired instrument 2 From the toolbar choose Simulate the current instrument The butt
15. 1 1 9 1 2 633 1 19 1 1 10 1 0 745 1 20 1 0 301 1 21 1 3 967 2 7 1 0 49 2 17 1 2 503 1 22 1 0 384 2 8 1 2 709 2 18 1 2 253 1 24 1 3 589 2 10 1 1 369 2 20 1 3 122 2 1 1 3 969 2 11 1 1 993 2 21 1 3 017 2 2 1 1 032 2 12 1 0 412 2 22 1 2 247 1 901 2 3 1 2 4 1 1 445 0 585 2 13 1_ 2 14 1 3 045 0 727 2 23 1 2 24 1 0 555 2 5 1 1 849 2 15 1 2 362 3 1 1 0 894 2 6 1 2 282 2 16 1 2 475 3 2 1 3 842 3 3 1 2 873 3 4 1 1 537 3 6 1 1 511 3 7 1 1 397 3 8 1 2836 1 212 3 9 1 3 10 1 1 689 3 11 1 1 433 3 12 1 2 735 3 13 1 0 226 3 14 1 1 956 3 16 1 0 029 3 17 1 3 363 3 18 1 2 175 2464 3 19 1 3 20 1 0 367 3 21 1 1 312 3 22 1 1 552 3 23 1 3 818 3 24 1 0 35 4 2 1 0 254 4 3 1 1 592 4 4 1 2 456 1 65 4 5 1 46 1 0 259 4 7 1 2 709 4 8 1 3 972 4 9 1 1 519 410 1 4 2 08 4 12 1 1 051 4 13 1 1 093 4 14 1 0 325 2 935 4 15 1 4 16 1 Intemational Laboratories 2 077 4 17 1 2 571 4 18 1 Figure 6 10 Previewing a measurement results printout Multimode Detection Software User s Manual 6 144 Viewing Measurement Results 3 In Print Preview choose Print to print out the measurement results 4 In Print Preview choose Close to close the window Note
16. ADC overflow Make sure the correct filter slides are installed in the instrument and check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides Beckman Coulter Inc Error Code Software Error Codes A 3 Table A 1 Software Error Codes and Solutions Error Message LED energy too low Recommended Solution Make sure the correct filter slides are installed in the instrument and check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 63 No LED for selected filter installed Contact a Beckman Coulter Service Engineer 64 ADC gain cannot be maintained Contact a Beckman Coulter Service Engineer 65 LED gain setting failed Contact a Beckman Coulter Service Engineer 66 ADC gain fluctuated during measurement Run the measurement again correction of PMT counts is not possible Try measurement again 67 Reference ADC too high for valid Make sure the filter compartment and luminescence measurement microplate chamber doors are closed 68 PMT dark counts exceed valid range for Make sure the filter compartment and measurement Check threshold or ensure that microplate chamber doors are closed flaps are closed 69 Reference ADC LED too low retry Run the measurement again measurement 140 Command canceled Aborted
17. B14 Allocation Sample Sampie Sample 3 T a7 S17 3 TT T T3 TF T5 TS T7 E TS Sample_ Sample Sample_ Sample D T 7 3 Sample Sample Sample Sample Sample Sample Sampie Sample Sample 6 Sample Sample Sample Sample Sample Sample Sample Sample Sampie Sample Sampie Sample Sample Sample Sample International Laboratories Tnternational Laboratories Figure 5 24 Previewing a protocol configuration and sample layout printout 4 In Print Preview choose Print to print out the measurement results tal 5 In Print Preview choose Close to close the window Note Choosing Close without first choosing Print cancels the printout Beckman Coulter Inc Defining Editing and Running Protocols 5 33 5 8 Exporting and Importing Protocols User defined protocols saved in the Protocol Selection List can be exported to an XML file and imported later to restore that configuration or share it with a copy of Multimode Detection Software installed on another computer An exported XML file also saves detection method s and labware configurations used in the protocol Exporting default protocols included with the software installation is not necessary Since default protocols may not be edited deleted or overwritten they are always available to users at all times Because of this importing a default protocol from an XML export
18. Choosing Close without first choosing Print cancels the printout Beckman Coulter Inc A 1 Codes A 1 Appendix A Software Error Troubleshooting Software Error Codes Table A 1 lists software error codes error messages and recommended solutions for resolving errors Table A 1 Software Error Codes and Solutions Error Message Recommended Solution Commanded transport motion exceeds actual physical travel limits Check plate parameters configured in Labware Information refer to Section 4 2 1 Defining Labware Information Auto calibration ramp parameter exceeds valid range Make sure the microplate carrier movement is not obstructed and run the measurement again Transport is not operational or the transport sensor is defective Make sure the transport lock has been removed from the microplate carrier Transport lost more than 2 full steps Make sure the microplate carrier movement is not obstructed Calibration positioning failed N A Offset calculated in auto calibration is out of expected range Possible causes ADC values are too low lamp is not adjusted incorrect EEPROM parameters Check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides Offset calculated in auto calibration is out of expected range Possible causes ADC values are too low lamp is not adjusted incorrect EEPROM paramete
19. Current labware lot and choose True if necessary True must be selected in order to save changes made to the labware lot In the property grid edit lot dimensions and information as desired Refer to Section 4 2 2 Configuring Offsets and Well Dimensions for the Default Labware Lot for more information about the fields available in the property grid Edit the lot Notes if desired Choose Save to save changes made in the Labware Editor OR Choose Back to view or edit Plate Information refer to Section 4 3 1 Viewing and Editing Labware Information OR Choose Cancel to close the Labware Editor without saving changes Multimode Detection Software User s Manual 4 10 Creating and Editing Labware 4 4 Copying Labware Beckman Coulter Inc Labware can be copied and then used as a template for a new labware type by editing the dimensions and parameters in the Labware Editor refer to Section 4 3 Editing Labware To make a copy of a labware type 1 In the Labware Selection List select the labware type to copy 2 From the toolbar choose Make a copy of the selected labware OR From the Actions menu choose Make a copy of the selected labware OR Right click on the selected labware type and choose Make a copy of the selected labware from the menu that appears Note The default name format for copied labware types is Copy of OriginalName To change the name edit the labware type refer to Section 4 3
20. Detection Software Setup ps ioj xi Language Options a Operating System Language Selection Fy If the Multimode Detection Software is being installed on a German language Operating System check the box below J German Language Operating System Nullsoft Install System v2 0b4 Figure 1 3 Installer Language Options 4 If installing the software on a German language version of Windows choose German Language Operating System This ensures that all software screens are displayed correctly when German is set as the system language used in the Multimode Detection Software interface 5 Choose Install to start the installation Multimode Detection Software Installation appears Figure 1 4 Multimode Detection Software Installation E x 2 To start Multimode Detection Software Setup click OK To quit without installing click Cancel cme Figure 1 4 Starting the software installation Beckman Coulter Inc installing Setting Up and Using the Software 1 7 6 Choose OK to start the installation The Multimode Detection Software setup wizard appears Figure 1 5 j Multimode Detection Software Welcome to the Multimode Detection Software Setup Wizard The installer will guide you through the steps required to install Multimode Detection Software on your computer WARNING This computer program is protected by copyright law and international treaties Unauthorized duplication or dis
21. Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 4 In Integration Time seconds enter the length of time in seconds the signal is collected from samples The integration time may be set within the range of 0 00005 to 3600 seconds 5 Choose Save to save the new fluorescence intensity bottom detection method The new method appears in the Detection Method Selection List OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without saving the new detection method Multimode Detection Software User s Manual 3 10 Creating and Editing Detection Methods 3 2 3 5 Defining Fluorescence Polarization Method Parameters 880 only A fluorescence polarization method performs a fluorescence intensity measurement where two orthogonal perpendicular polarization states are measured A light source above the plate passes through an excitation filter which passes only the wavelength necessary for excitation and a polarizing filter The fluorescence resulting from the excitation of the sample is separated by polarization and passed through two emission filters equipped with polarizing filters to ensure both polarization states are measured The polarized signal is then read from above the plate by the photo multiplier tube 85 Method Editor Method Parameters Method Technique Method
22. Length 12 77 Reading height 2 422 g ri d width 8 57 B well Info Columns 12 Rows 8 Luminescence LuminescenceGlow LuminescenceFlash BioLuminescenceRET C FluorescenceRET O FluorescencelntensityTop C FluorescenceIntensityBottom C FluorescencePolarization TimeResolvedFluorescence Well bottom shape Flat Well shape Round Well volume 300 Piate Name Information about the Name of this labware i field being configured Notes None Cancel Figure 4 2 Defining plate dimensions and information Multimode Detection Software User s Manual 4 4 Creating and Editing Labware To define Labware Information 1 Ifnecessary click the next to Labware Info to display the fields in the category 2 Enter the Plate Name A name must be entered to proceed to the second configuration screen Labware Lots 3 Ifnecessary click the next to Labware Measurements to display the fields in the category Note More information about the field being defined is displayed below the property grid Figure 4 2 CAUTION The plate height configured must not be less than that of the A N actual plate Doing so may cause the DTX 880 optics transport to collide with the plate during a Read Height Optimization Enter the Height of the plate AI labware dimensions are entered in centimeters Enter the Height with lid Enter the Length of the plate NY D
23. Parameters gt Method Name Polarization Beckman Coulter Inc Excitation Filter nm 485 SL 1 Emission Filter nm 535 SL 1 Integration Time seconds o oo Date created Cancel Figure 3 8 Defining fluorescence polarization method parameters To define fluorescence polarization parameters 1 Enter a Method Name Figure 3 8 2 Select the Excitation Filter nm 3 Select the Emission Filter nm Note The filters available are those installed on the excitation and emission filter slides loaded in the instrument and configured for fluorescence polarization techniques in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 4 In Integration Time seconds enter the length of time in seconds the signal is collected from samples The integration time may be set within the range of 0 00005 to 3600 seconds 5 Choose Save to save the new fluorescence polarization detection method The Creating and Editing Detection Methods new method appears in the Detection Method Selection List OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without saving the new detection method 3 2 3 6 Defining Time Resolved Fluorescence Method Parameters 880 only In a time resolved fluorescence measurement the excitation light source is turned off and the measureme
24. Port 1 free serial port Operating Systems Windows NT 4 Service Pack 6a Windows 2000 Windows XP Operating System Languages English U S German Web Browser Internet Explorer 5 01 or later 1 3 2 Using the Setup Program to Install the Software The setup program on the installation CD installs all of the components required to run Multimode Detection Software Note The setup program also provides the ability to repair the software installation or remove uninstall the software from the host computer refer to Section 1 3 3 Repairing or Removing the Multimode Detection Software Installation To install Multimode Detection Software 1 Exit all open Windows programs before running the setup program 2 Make sure the current user account has Administrator privileges Accounts with Standard or Restricted access are not permitted to run the setup program Contact the site system administrator for more information about account privileges Multimode Detection Software User s Manual a 1 6 Installing Setting Up and Using the Softwar 3 Insert the Multimode Detection Software installation CD into the CD ROM drive After a few seconds Language Options appears Figure 1 3 Note IfLanguage Options does not appear automatically within a few seconds of inserting the CD into the drive use Windows Explorer to browse to the CD ROM drive and run MultimodeDetectionSoftwarelnstall exe amp Multimode
25. Selecting the Detection Method Section 4 6 1 e Preparing and Loading the Labware Section 4 6 2 Performing the Optimization Read Section 4 6 3 Selecting the Centers of the Four Corner Wells Section 4 6 4 Verifying Well Centers Section 4 6 5 To optimize labware 1 In the Labware Selection List select the labware to optimize 2 From the toolbar choose Optimize the selected labware OR From the Actions menu choose Optimize the selected labware OR Right click on the selected labware and choose Optimize the selected labware from the menu that appears Beckman Coulter Inc Creating and Editing Labware 4 13 4 6 1 Selecting the Detection Method Labware is optimized by performing absorbance measurements of the four corner wells of the microplate and then defining the well centers using images of the wells generated by the measurements To ensure the most accurate optimization is performed use Select Detection Method to select the most appropriate detection method for the optimization Figure 4 7 an Optimizing Labware BCLumi96 o x Select a Detection Method Prepare the Labware Optimize Select Center of Left Top Well Select Detection Method Select a detection method to proceed The detection method should use the same technique that will be used in the target assay Click Next to proceed Name Measurement technique Measurement type Created Last Edited
26. and well dimensions Figure 4 3 The offsets and dimensions entered when new labware is created define the default labware lot DefaultLot After the new labware has been saved additional lots may be created by optimizing the labware to compensate for dimensional variations between different production lots refer to Section 4 6 Optimizing Labware In Labware Lots x and y offsets are defined for all four corners of the labware An x offset is the distance from the edge of the microplate to the first row a y offset is the distance from the edge of plate to the first column Well dimensions defined include well depth length and width as well as distances between rows and columns To configure offsets and well dimensions 1 If necessary click the next to Labware Lot Measurements to display the fields in the category More information about the field being defined is displayed below the property grid Figure 4 3 Note The fields in Labware Lot Info may not be configured when creating new labware Multimode Detection Software User s Manual Creating and Editing Labware 2 Enter column and row x and y offsets for each of the four corner wells A offsets and well dimensions are entered in centimeters d Labware Editor SemiNewLabware x Labware Lots Labware Information Labware Lots gt DefautLot x B Labware Lot Info Current labware lot True Date edited Instrument Name Optimized Landsca
27. by user N A 141 Measurement time exceeds limit for current Use a shorter measurement time in the measurement method detection method configuration 142 Current measurement incomplete wrong Check the parameters configured in the command received protocol 144 Instrument is busy Wait until the amber LED on the instrument is off If instrument is still busy when the LED is off turn the instrument off and on 400 Unknown PIC error Contact a Beckman Coulter Service Engineer 401 Communication timeout with PIC controller Contact a Beckman Coulter Service Engineer 403 PIC timeout during a measurement or Contact a Beckman Coulter Service Engineer initialize 410 Number of steps are zero Check the parameters configured in the protocol 411 LED transport commanded to move to a Contact a Beckman Coulter Service Engineer position less than 0 412 Shutter transport commanded to move to a Contact a Beckman Coulter Service Engineer position less than 0 413 Z transport commanded to move to a position Contact a Beckman Coulter Service Engineer less than 0 415 LED transport commanded to move to a Contact a Beckman Coulter Service Engineer position greater than maximum travel 416 Shutter transport commanded to move to a Contact a Beckman Coulter Service Engineer position greater than maximum travel Multimode Detection Software User s Manual A 4 Software Error Codes Table A 1 Sof
28. files Figure 1 15 Choosing the Simulated Data files To choose different simulated data files 1 Choose the Simulated Data tab Simulated Data appears Figure 1 15 2 In the desired field enter the full path to the new simulated data file for example c program files beckman coulter inc multimode detection software templates DefaultDataFile dat Any data file with the dat extension may be selected including prior measurement results Proceed to step 5 OR Choose the Browse button next to the desired measurement type Open appears Note Simulated data files are used when the number of measurement points in the simulated protocol run is the same as those present in the data file When the number of measurement points is different the software generates random data 3 In Open browse to desired data file Any data file with the dat extension may be selected including prior measurement results Beckman Coulter Inc Installing Setting Up and Using the Software 1 19 4 Choose Open to select the data file and return to Software Settings OR Choose Cancel to close Open without selecting a different data file 5 Repeat steps 2 4 for each simulated data file desired to change 6 Choose Apply to set the new default data file s 1 4 2 4 Choosing the Directory Where Measurement Results are Saved All exported measurement results files regardless of format are saved in a single directory The default s
29. from the desired filter slide control section to unload the filter slide from the filter compartment and partially open the compartment door Note To remove the filter slide it is still necessary to grasp it by the tab and pull it until it is free of the geared track Store the removed filter slide in a protected dust free area preferably in the original packaging e Choose Load from the desired filter slide control section to retract the filter slide into position Beckman Coulter Inc Creating and Editing Detection Methods 3 1 Overview Multimode Detection Software stores measurement configuration parameters in detection methods Stored parameters include the method technique for example absorbance filter s used and parameters specific to the selected method such as integration time Absorbance luminescence and fluorescence method techniques are all supported The method techniques available to the user depend on the capabilities of the instrument being controlled Detection methods are created and edited using the Method Editor and stored in the Detection Method Selection List Once a detection method has been defined it may be used in a measurement protocol refer to Chapter 5 Defining Editing and Running Protocols Multimode Detection Software User s Manual 3 2 Creating and Editing Detection Methods To view available detection methods and access the Method Editor From the navigati
30. main SCreeN cccecceeceeseeeeceteceeeeeeaeecsaeeneenees 1 11 Figure 1 9 Warning Instrument is not connected cceccceceseceseceececeseceseceeeeeeseecseeeneeees 1 12 Figure 1 10 Instrument Selection List with simulated instrument selected eeeeeeeeeees 1 13 Figure 1 11 Configuring communications settings 0 c ccceesseeeceecceeeeceeeceeeeeeeeeseecsaeeneenees 1 14 Figure 1 12 Configuring Software Settings sass cessiasseasisssvsncssaanseasssosevanssanveaives dannsssaveenssesisastnas 1 15 Figure 1 13 Choosing the software interface language cceccesscesecseeeceeeeeeeeceaeceeeeaeeeeeenees 1 16 Figure 1 14 Configuring Print Settings sccecstevcxsunvcncuseancncaniecdaxcanansdencannaduactauntaxetsuatencarensntensatined 1 17 Figure 1 15 Choosing the Simulated Data files 00 0 cece essssecssecoseessseseescensessceseesoesoncensceneees 1 18 Figure 1 16 Choosing the directory where data is SaVCd escesesseeeseeeeeeeeeeceaeceeeeeeeneeenees 1 19 Figure 2 1 Instrument Selection List lt sscaansceacsvundenaccesstacencnsencanascuacesindanassuasantentenntactsngvacarmadenteste 2 2 Figure 2 2 Instrument Settings Basic Settings cssccssscssssssecsssscssccssscssseccsnnesersssncesensens 2 6 Figure 2 3 Instrument Settings Filter Slides scsasssnscacranssesvarveoransvaascaenvendsuanwannseansieorcaspronnssanys 2 8 Figure 2 4 Configuring filter properties lt 2 jscssussascssscpescsnshsdecteata
31. may be configured When Read by well is selected omy area Resolution mm Selected Scan Points fa 2248 Points x 14 Points Y 14 Well boundary Measurement points Click and drag until the desired number of points is selected OK Cancel Figure 5 9 Configuring an area scan 12 In Resolution mm use the up and down arrows to choose the proximity of measurement points Choosing a smaller value increases the number of measurement points available choosing a larger value decreases the number of points available Available resolutions are determined by the type of labware selected and configured in the protocol Refer to Sections 5 2 3 Selecting the Labware Type Used in the Protocol and 5 2 4 Configuring Labware Layout Settings for more information Multimode Detection Software User s Manual 5 12 Defining Editing and Running Protocols 13 To configure an area scan click anywhere inside the well boundary except the center row and drag until the desired number of measurement points is selected Figure 5 9 OR To configure a linear scan click anywhere on the center row or column inside the well boundary and drag towards the boundary until the desired number of measurement points is selected Figure 5 10 Note The type of scan that may be configured is determined by the Reading Direction Settings in Layout Settings refer to Section 5 2 4 Configuring Labware Layo
32. measured wells on the plate Figure 6 8 When multiple measurement cycles were performed in a protocol run data from each cycle is presented in a separate worksheet gt DTX 880 Fluorescence polarization measurement results contain raw data and calculated mP refer to Section 6 3 1 2 1 Viewing Fluorescence Polarization Results 880 only Ea Microsoft Excel Basic Endpoint Measurement Fluorescein 96 _Fluorescein Top 0_04 23 2004_01 26 06 40 xml File Edit View Insert Format Tools Data Window Help DESA 64 ABE S naa 4 FZ Me 44 20 B p E Arial Unicode mS e Overflow Temperature Error Time seconds MatrixFormat Incorrect OK 6 onan kwn a OROROORO PRR EEE EE PRR ER EE SH coco cocoon Figure 6 8 Viewing absorbance data in a Cycle worksheet excerpt To view results for a measurement cycle In Excel choose the Cycle tab for the desired measurement cycle Multimode Detection Software User s Manual 6 12 Viewing Measurement Results 6 3 1 2 1 Viewing Fluorescence Polarization Results 880 only Fluorescence polarization measurement results exported to Excel contain raw data measured at both the parallel and perpendicular polarization planes and calculate an approximate mP for each sample Figure 6 9 Data is laid out in matrices that represent the wells on the plate When multiple measurement cycles were performed in a protocol run data from each cycle is presented in a se
33. of such components and accessories is maintained at the factory b The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company will give reasonable assistance to Purchaser in obtaining from the manufacturer s own warranty c Any product claimed to be defective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportation charges collect unless the product is found to be defective in which case the product must be properly decontaminated of any chemical biological or radioactive hazardous material d The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company e Ifthe product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out o
34. or 48 well plates at low VAN speed only Shaking low density plates at higher speeds may cause liquid in wells to spill 7 In Shake Intensity select the desired intensity of shaking Low Medium or High 8 In Shake Interval enter the length of time in seconds 0 60 to shake the microplate 9 In Shake Mode select the desired shaking pattern e Linear shakes from side to side e Orbital shakes labware in a circular pattern e Squared shakes labware in a square pattern moving at right angles 10 To configure an area or linear scan click in Scan and select Yes Area scans read a number of measurement points arranged in a grid pattern across each well Linear scans read a number of points in a linear axis crossing the center of each well Scan measurements are not available when a kinetic measurement is configured Note Scans may only be performed on samples on plates with 96 or fewer wells Note Ifa scan measurement is not desired proceed to step 15 Beckman Coulter Inc Defining Editing and Running Protocols 5 11 J 11 To configure the type of scan measurement and the number of points measured click in Scan Points and choose the configuration button Scan Selection Editor appears Figure 5 9 Scan Selection Editor xj Select the scan resolution and measurement points to read The type of linear scan trg direction is conhgurod m s A tal linear scan may be configured Resolution mm
35. s Manual e 1 12 Installing Setting Up and Using the Software 2 Ifthe Protocol Selection List Figure 1 8 appears immediately the instrument was automatically detected by the software Proceed to Section 1 4 2 Configuring Software Settings to configure system settings OR If Warning appears Figure 1 9 the instrument was not detected by the software Choose OK to work in simulation mode until the communications settings are configured properly HE Warning Instrument is not connected Entering simulation mode Il Don t show this message again Figure 1 9 Warning Instrument is not connected 3 Ifthe instrument was not detected configure the communications settings following the steps in Section 1 4 1 Configuring Communication Settings 4 Configure system settings such as language and printing options following the steps in Section 1 4 2 Configuring Software Settings Beckman Coulter Inc Installing Setting Up and Using the Software 1 13 1 4 1 Configuring Communication Settings After physically connecting the instrument to a serial communications port on the host computer the selected serial port and communications speed must be configured correctly before Multimode Detection Software can control instrument operations Communications settings are configured in Instrument Settings Before communications with an instrument are configured a simulated instrument Seria
36. the Software 1 1 1 2 1 3 1 4 OVET VIEW 54 55 gia ae ey ead CSP e E a E E E E eee ese A a pna a S aa 1 1 Using Multimode Detection Software 0 0 ee eee 1 2 1 2 1 Launching the Software 22 lt 4 ves oceeckesadneeteearneadeesbueas 1 2 1 2 2 Using the Software Interface 64 4 0k 4 sxe conse Mee ee 5S Hae SRY 1 3 1 2 2 1 About the Navigation Pane 0000 e eee eee 1 3 1 2 2 2 About the Toelbars coscctodatane oda dese god padw ais 1 3 1 2 2 3 About the Selection and Configuration Pane 1 3 1 2 3 Accessing Online Help ij 2144c4asvessnne carded Sete edeeas 1 4 Installing Multimode Detection Software 0 0 cee eee eee eee 1 5 1 3 1 Meeting System Requirements lt 3 icc 5542454 0x00d600 baeadeaduuns 1 5 1 3 2 Using the Setup Program to Install the Software 1 5 1 3 3 Repairing or Removing the Multimode Detection Software Installation 1 9 Setting Up Multimode Detection Software 0 0 eee eee 1 11 1 4 1 Configuring Communication Settings 0c ee eee 1 13 14 2 Configuring Software Settings 0 cece eee eee eee 1 15 1 4 2 1 Setting the System Language 0 00 1 16 1 4 2 2 Configuring Print Settings 1 17 1 4 2 3 Choosing Simulated Data Files 0 5 1 18 1 4 2 4 Choosing the Directory Where Measurement Results ate SAVED owe abcd prene keai iE hohe ead EER 1 19 Multimode Detection Software U
37. the cycle appears in the right pane OR For linear and area scan measurements choose the desired sample Raw data measured at each point within the chosen sample appears in the right pane 3 If desired right click on the raw data results and choose Copy to copy the raw data values to the clipboard The values may then be pasted into another document or file 4 Choose a different cycle or sample to view OR Choose a different results tab to view OR Choose Finish to close Run Protocol Measurement results are saved and or prepared for printing when file and print options are defined in the protocol refer to Section 5 2 6 Configuring Output Settings Multimode Detection Software User s Manual 6 6 Viewing Measurement Results 6 2 3 Viewing Kinetic and Scan Graphs Graphs displays graphs for each sample measured in kinetic area scan and linear scan protocols Figure 6 4 The tab is not displayed unless a kinetic or scan measurement is performed Z Run Protocol 340 Scan Run Protocol Prepare to Run Prato Run Protocol gt Click Finish to export data APM ap gp a Mg yp GY py gp gw yey mp yw yp pp PY wp gw Ep yeep a Estimated Time 00 22 Measurement Time 00 39 State RawData Graphs Figure 6 4 Viewing area scan measurement graphs Graphs for individual samples can be viewed in detail To view a detailed graph of an individual sample Click on the desired samp
38. valid range 0 15 Contact a Beckman Coulter Service Engineer 441 LED not initialized Contact a Beckman Coulter Service Engineer 442 The LED is not powered Contact a Beckman Coulter Service Engineer 443 The LED has exceeded maximum allowed power consumption Contact a Beckman Coulter Service Engineer 444 LED power is low Contact a Beckman Coulter Service Engineer 445 LED power overflow Contact a Beckman Coulter Service Engineer 446 No LED installed Contact a Beckman Coulter Service Engineer 450 No temperature installed Check instrument configuration 451 Temperature setpoint exceeds maximum valid setpoint Decrease the temperature setting refer to Section 2 3 3 1 Temperature Control 880 only 452 Temperature sensor is not responding Check the current temperature setting refer to Section 2 3 3 1 Temperature Control 880 only 453 Temperature sensor not found Decrease the temperature setting refer to Section 2 3 3 1 Temperature Control 880 only Beckman Coulter Inc Index A absorbance detection methods configuring 3 6 selecting type 3 4 area scan measurements configuring 5 10 viewing graph 6 7 Cc communication settings configuring 1 13 copying detection methods 3 15 labware 4 10 protocols 5 29 creating detection methods 3 3 labware 4 3 protocols 5 3 D de
39. 1 If desired the offsets distances and lot name may be edited in Verify Well Centers 0 x Verify Well Centers Verify well measurements below Click save to save optimization or back to adjust values graphically Select a Detection Method Prepare the Labware Optimize Select Center of Left Top Well Select Center of Left Bottom Well Select Center of Right Top Well Select Center of Right Bottom Well Verify Well Centers gt Lower right y offset 1 123 Property grid B Labware Lot Measurements Column distance 0 9 Lower left x offset 1 438 Lower left y offset 1 123 Lower right x offset 1 438 Row distance 0 9 Upper left x offset 1 438 P Upper left y offset 1 123 Upper right x offset 1 438 Upper right y offset 1 123 E Labware Lot Name Lot ID Name Lot 2004 04 06T11 12 14 Column distance Information about the Distance between columns cm field being confi gure d Cancel Figure 4 11 Verifying well centers To verify and edit offsets distances and lot name 1 If necessary click the next to Labware Lot Dimensions to display the fields in the category Note More information about the field being defined is displayed below the property grid Figure 4 11 In Column distance verify the distance between columns and edit the dimension All offsets and well dimensions are entered in centimeters if desired Verify the x and y offsets for th
40. 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 1 28 2004 4 5 2004 4 5 2004 Figure 3 1 Accessing detection method functions Exporting and Importing Detection Methods Section 3 6 3 2 Creating and Editing Detection Methods 3 3 Creating Detection Methods Detection methods are created in the Method Editor which guides the creation process with a wizard type interface Creating a new detection method requires e Selecting a Method Technique Section 3 2 1 Defining Method Parameters Section 3 2 3 Note When creating absorbance measurements a third screen Method Type must also be configured refer to Section 3 2 2 Selecting the Type of Absorbance Method To create and define a new detection method From the toolbar choose Create a new method OR From the Actions menu choose Create a new method OR Right click in the Detection Method Selection List and choose Create a new method from the menu The Method Editor appears Figure 3 2 3 2 1 Selecting a Method Technique In the Method Editor the type of detection method to create is selected in Method Technique Figure 3 2 Only techniques supported by the instrument currently selected in the Instrument Selection List are available for configuration refer to Chapter 2 Setting Up and Controlling Instruments Method Editor Method Technique Method Technique gt M
41. 3 Select Basic Settings to display the Basic Settings tab if necessary 4 In Port select the serial communications port on the host computer that connects to the instrument 5 In Baudrate select 38400 6 Choose Apply to detect the instrument When the instrument is detected the instrument information fields are autopopulated with information about the connected instrument Refer to Table 2 1 for more information about each field 7 Choose OK to close Instrument Settings Beckman Coulter Inc installing Setting Up and Using the Software 1 15 1 4 2 Configuring Software Settings Multimode Detection Software can be customized using the options available in Software Settings Figure 1 12 Use the tabs in Software Settings to configure the system language printout options default simulated data files and directory where measurement results are stored To configure Software Settings 1 From the navigation pane choose Software Settings Software Settings appears Figure 1 12 a5 Multimode Detection Software Connected Fitter Slides Ex 2 Em 1 File Actions Help Software Settings Language print Settings simulated Data Directory Settings Name Culture File German Germany Deutsch Deutschland Apex de DE resources German Austria Deutsch Osterreich Apex de AT resources English United States English United States Apex en US resources NY q Software Settings Software set
42. 33 226 9134 200 2697 968 2394 236 6952 571 8421 852 3499 8 1123030 2937941 3773845 517249 2960518 1324213 440642 3989441 8 2527563 3119224 1983752 2122637 1868113 507987 1698008 3114636 165 0444 206 036 508 4003 435 7132 437 5802 615 7166 409 8836 347 6583 3 1839858 488922 262697 843349 1835794 3289586 3966519 374122 3 1501256 1317362 125762 1187665 3834173 2300031 3877177 3145153 3 328 112 251 0832 542 2399 67 73224 128 506 395 1804 245 3028 333 7325 14520 616583 2884305 3859345 1009347 2793620 982926 1316235 10 150524 3273950 403026 3006597 1918844 94093 1798485 2163633 10 960 1014 534 0269 840 5477 348 6114 82 00718 959 0894 62 98167 150 3441 1310257 2966643 838280 1039214 2791205 1246257 1582394 3562401 n 3478199 3784489 1576985 1791610 2239292 488748 2731733 1185695 n 244 0986 116 7432 _ 76 78958 _ 33 29479 335 6434 i 608 8201 33 96768 657 8323 t Figure 6 9 Viewing fluorescence polarization data excerpt Viewing Measurement Results 6 13 6 4 Printing Measurement Results When a protocol has Print options configured in Output Settings the measurement results are available to print after completing a protocol run Refer to Section 5 2 6 Configuring Output Settings for information about the printing options available Note Dependi
43. 4 Greiner 96 Round PP 96 21812004 21812004 218 2004 Greiner 96 Round PS 96 21812004 21812004 218 2004 Greiner 96 Thermal Cycler 96 2 8 2004 2 8 2004 2 8 2004 Greiner Flat 384 Square 384 21812004 21812004 218 2004 Greiner Shallow 384 Round 384 21812004 21812004 218 2004 X ps Software Settings Figure 4 1 Accessing labware functions Beckman Coulter Inc Creating and Editing Labware 4 3 4 2 Creating Labware New types of labware are created in the Labware Editor which guides the creation process with a wizard type interface Creating labware includes Defining Labware Information Section 4 2 1 Configuring Offsets and Well Dimensions for the Default Labware Lot Section 4 2 2 To create and define new labware From the toolbar choose Create a new piece of labware The Labware Editor appears Figure 4 2 OR From the Actions menu choose Create a new piece of labware OR Right click in the Labware Selection List and choose Create a new piece of labware from the menu 4 2 1 Defining Labware Information Use Labware Information to define labware names dimensions well parameters and supported measurement techniques Figure 4 2 8S Labware Editor Labware Information Labware Lots E Labware Info Date created 4 4 2004 Date edited 4 4 2004 Absorbance Supported techniques Plate Name E Labware Measurements Height 1 4 Height with lid 2 222 P rope rty
44. 4 MJ Microseal 384 384 Nunc 24 24 Nunc 6 6 Nunc Flat 384 Black 384 Nunc Flat 384 Square 384 Nunc Flat 384 384 SBS Flat 384 Round 384 SBS Flat 384 Square 384 5B5 Flat 96 Round 96 Standard 384 384 Standard 96 96 Figure 5 4 Selecting the type of labware used in the protocol Multimode Detection Software User s Manual 5 6 Defining Editing and Running Protocols To select labware 1 Select the desired Type of Labware from the list 2 Choose Next to configure the plate layout refer to Section 5 2 4 Configuring Labware Layout Settings OR Choose Back to change the measurement technique performed by the protocol refer to Section 5 2 2 Selecting the Technique Type OR Choose Cancel to close Labware Selection without saving the new protocol 5 2 4 Configuring Labware Layout Settings Use Layout Settings to configure how wells on the plate are read Figure 5 5 Settings are configured in two tabs e Layout Selection Settings configures which wells are read in the measurement Figure 5 5 e Reading Direction Settings configures the order of how wells are read row by row column by column or well by well Figure 5 6 To configure labware layout settings 1 In Layout Settings choose the Layout Selection Settings tab to view the layout of the selected labware Figure 5 5 Layout Settings General Settings Layout Selection Settings Reading Direction Settings gt PAS Wasi fc
45. 4 2 1 1 General Labware Selection Guidelines 4 5 4 2 2 Configuring Offsets and Well Dimensions for the Default Labware Lot 4 5 4 3 Editing LabWatevc 46 ince ens Sale kene dad VEEG EERE EREL EERTE Balhae 4 7 4 3 1 Viewing and Editing Labware Information 4 8 4 3 2 Selecting and Editing Labware Lots 0 0 0000 e eae 4 9 44 Copying Labware c oa0asd bed a hd deat Geese OiS A EO RRR LER REE R ER 4 10 Ao Deleting LabWates ccs 20ae cnn iinet eager San a Sadak ped Bek EEEE ges 4 11 4 6 Optimizing Labware naasa unanenn neren 4 12 4 6 1 Selecting the Detection Method uasanaanan nanan ee ee eee 4 13 4 6 2 Preparing and Loading the Labware 0 00000005 4 14 4 6 3 Performing the Optimization Read 0 00 0 0 cee eee 4 15 4 6 4 Selecting the Centers of the Four Corner Wells 4 16 46 0 Verifying Well Centers cidiasihccdann eR addaneidandtinavrdas 4 17 4 7 Exporting and Importing Labware 0000 00 cece eee eee 4 19 5 Defining Editing and Running Protocols 5 1 OVETVIEW 6 649 49 40 oS aeie a eg e a E a e a e E aed 5 1 5 2 Creating Protocols u esrdenie owed autos oho sad a i e a E Baa E ne dees 5 3 5 2 1 Configuring General Settings jock ean cae es nananana 5 3 5 2 2 Selecting the Technique Type 0 nnana 5 4 5 2 3 Selecting the Labware Type Used in the Protocol 5 5 5 2 4 Configuring Labware Layou
46. 5 1 Protocol Selection List Beckman Coulter Inc Defining Editing and Running Protocols 5 3 5 2 Creating Protocols New protocols are defined in Create Protocol which guides the creation process with a wizard type interface Creating a new protocol requires e Configuring General Settings Section 5 2 1 Selecting the Technique Type Section 5 2 2 Selecting the Labware Type Used in the Protocol Section 5 2 3 e Configuring Labware Layout Settings Section 5 2 4 e Configuring Detection and Preparation Methods Section 5 2 5 Configuring Output Settings Section 5 2 6 To create and configure a new protocol From the toolbar choose Create a new protocol x OR From the Actions menu choose Create a new protocol OR Right click in the Protocol Selection List and choose Create a new protocol from the menu Create Protocol appears Figure 5 2 5 2 1 Configuring General Settings Use General Settings to define the protocol name and enter any related notes about the protocol Figure 5 2 85 Create Protocol Absorbance 260 E xj General Settings General Settings gt Technique Type Please enter a name and notes for this protocol Click Next to proceed Labware Selection Layout Settings Method Selection Output Settings Protocol name Absorbance 260 Date created Wednesday April 14 2004 Date edited Wednesday April 14 2004 Date last run Wednesday April 14 2004 Notes B
47. 5 16 Defining Editing and Running Protocols 3 In Shake Interval enter the length of time in seconds 0 60 to shake the microplate 4 In Shake Mode select the desired shaking pattern e Linear shakes from side to side e Orbital shakes labware in a circular pattern e Squared shakes labware in a square pattern moving at right angles FA 5 To move the Shake preparation method to a different location in the execution aga Beckman Coulter Inc sequence in Method Selection select the method and choose Move the selected method forward or Move the selected method backward OR In Method Selection right click on the Shake preparation method and choose Move Up or Move Down 5 2 5 2 2 Configuring a Set Temperature Preparation Method 880 only A Set Temperature preparation method sets the temperature inside the microplate chamber The temperature is set by heating the microplate chamber cooling the chamber is not supported by the instrument Depending on the measurement wavelength and light source used in the protocol the set temperature may range from 3 C 5 4 F or 4 C 7 2 F above ambient to 45 C 113 F It takes a minimum of 30 minutes for the instrument to reach the desired temperature The actual time required depends on the relative change in temperature When a protocol with a Set Temperature preparation method is run the protocol does not pause to wait for the desired temperature to be rea
48. A numerical code used by the software to identify the instrument type Firmware Version The version of firmware loaded for the instrument PIC FW Version The version of firmware loaded for the PIC processor of the instrument Features The types of measurements the instrument is capable of performing Multimode Detection Software User s Manual 2 8 Setting Up and Controlling Instruments 2 3 2 Defining and Editing Filter Slides The Filter Slides tab Figure 2 3 is used to add remove and configure filter slides and the filters installed on a filter slide Filters used to perform measurements are mounted on two types of interchangeable slides One slide is reserved for excitation filters used in absorbance and fluorescence measurements the other is used for emission filters used in fluorescence and some luminescence measurements Excitation and emission filter slides are different sizes to prevent them from being installed in the incorrect position Each slide can hold up to six filters When a slide is exchanged an identification code built into the slide allows the Multimode Detection Software to recognize the new slide and filter configuration When a slide with a new configuration is inserted or the filters on a slide change the slide must be configured in the Filter Slides tab Up to 31 excitation filter slides and 31 emission filter slides may be stored in Multimode Detection Software at on
49. Creating and Editing Detection Methods e Labware opens the Labware Selection List and provides the ability to create edit optimize copy and delete labware types refer to Chapter 4 Creating and Editing Labware Instruments opens the Instrument Selection List and provides the ability to manually control instrument functions such as shaking and configure communications settings and filter slides refer to Chapter 2 Setting Up and Controlling Instruments Software Settings configures software settings including system language print options default simulated data files and the directory where measurement results are saved refer to Section 1 4 2 Configuring Software Settings 1 2 2 2 About the Toolbar The toolbar provides easy access to common software functions Functions available in the toolbar are specific to the module chosen in the navigation pane For example Optimize Labware is only available when the Labware module is active 1 2 2 3 About the Selection and Configuration Pane The selection and configuration pane is the large pane to the right of the navigation pane Options available in this pane change depending on which module is currently selected in the navigation pane For example when Protocols is selected the Protocol Selection List is displayed which provides access to configured protocols and functionality specific to the Protocols module Multimode Detection Software Us
50. G bMS Sa KERR AEE GRO REE SEAR ASE AGAR AREER HAR HERES 3 1 3 2 Creating Detection Methods nunana ann unanenn 3 3 3 2 1 Selecting a Method Technique 00 n cee eee eee 3 3 3 2 2 Selecting the Type of Absorbance Method 00 3 4 3 2 3 Defining Method Parameters 24102 ai eeeneeseibacakeeaeuess 3 5 3 2 3 1 Defining Absorbance Method Parameters 3 6 3 2 3 2 Defining Luminescence Method Parameters 3 7 3 2 3 3 Defining Fluorescence Intensity Top Method Parameters 3 8 3 2 3 4 Defining Fluorescence Intensity Bottom Method Parameters 880 only 0 0 00 cece eee eee 3 9 3 2 3 5 Defining Fluorescence Polarization Method Parameters 880 only 00 0 c ee eee eee 3 10 3 2 3 6 Defining Time Resolved Fluorescence Method Parameters 880 only 164 3444 sc 040 eeneo es Hues 3 11 3 3 Editing Detection Methods 44 0b0c 44 0s aude amas hatte nehsed Onn oe anes 3 13 3 4 Copying Detection Methods 0 ccc cen eee 3 15 3 5 Deleting Detection Methods 2 25 4 4 saas sea nee ene kobe awa s Reale ees 3 16 3 6 Exporting and Importing Detection Methods 000000000 eee 3 17 Beckman Coulter Inc 4 Creating and Editing Labware 4 1 OVERVIEW soatidan daw PE KREACE SE REM RURAL A RRA EEN SERRA EERE 4 4 2 Cr ating LaQWares escret einsame rE E da evan i Reekeataandadekewaaes 4 3 4 2 1 Defining Labware Information 00 000 e eee eee eee 4 3
51. H WN A In Reading height enter the height from the top of the plate at which the plate is read 8 Enter the Width of the plate 9 If necessary click the next to Well Info to display the fields in the category 10 Enter the number of Columns on the plate 11 Enter the number of Rows on the plate 12 Click in either column of Well bottom shape then click on the down arrow and choose the shape of well bottoms Flat Cone or Round 13 Click in either column of Well shape then click on the down arrow and choose the shape of the wells Round Square or Cone 14 Enter the maximum Well volume in microliters 15 In Supported Techniques select all measurement techniques compatible with the plate being defined Labware may be used only in protocols that use a compatible measurement technique Note Refer to Section 4 2 1 1 General Labware Selection Guidelines for more information about choosing the appropriate labware for the desired techniques 16 In Notes enter information about the labware or configuration if desired 17 Choose Next to define the default row and column offsets and well dimensions for the labware type in Labware Lots refer to Section 4 2 2 Configuring Offsets and Well Dimensions for the Default Labware Lot OR Choose Cancel to close the Labware Editor without creating or saving new labware Beckman Coulter Inc 4 2 1 1 Creating and Editing Labware 4 5 General Labware Sele
52. Me to allow only the user account currently logged in to access and use the software Note Only user accounts with Administrator or Standard access may use Multimode Detection Software User accounts with Restricted access do not have access to the software Choose Next to continue the installation OR Choose Back to return to the previous screen in the setup wizard OR Choose Cancel to close the setup wizard without installing the software Follow the remaining instructions in the setup wizard to complete the software installation When installation is complete communications and system settings may be configured refer to Section 1 4 Setting Up Multimode Detection Software Installing Setting Up and Using the Software 1 9 1 3 3 Repairing or Removing the Multimode Detection Software Installation Along with performing the initial Multimode Detection Software installation the setup program also provides the ability to e Repair the installation if required software components or files are missing or damaged Try repairing the installation if the software will not open or does not run correctly e Remove uninstall Multimode Detection Software from the host computer Note Multimode Detection Software may also be removed from the computer using the Add Remove Programs control panel in Windows To repair or remove the software 1 Exit all open Windows programs before running the setup program 2 Make sure t
53. Saj kG iv 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Labware Selection m Layout Settings gt i Method Selection 8SSS 060860686 Output Settings 1BOSSSSSSROOOe Click a column header to select Click this button SSO6S8S8688668 all wells in that column to select all wells Q Q e eoeoeooooooeodo onthe plate SOSSOOCCOSC08 OSVOSVSSOSIESOSSS A ELLI S660 Selected SSOESe wells Technique Type Click a row header to select all wells in that row aal 2 3 ua R u ae a Cancel Figure 5 5 Selecting which wells are measured on a 384 well plate Beckman Coulter Inc Defining Editing and Running Protocols 5 7 2 Select the desired wells to read All wells on the plate click the small button in the upper left corner of the plate layout display All wells in a column click the desired column header Multiple columns may be selected All wells in a row click the desired row header Multiple rows may be selected Individual wells click each desired well Groups of wells click and drag over the desired group of wells Multiple groups may be selected Note Wells are deselected using the methods listed above for example clicking the header of a selected column deselects all wells in the column 3 Choose the Reading Direction Settings tab to configure the order in which wells are read in th
54. Sample Sample Sample Sample Sample Sample Date Last Used Wednesday April 14 2004 asais aoas ama Lami Garena Sevens Notes Basic absorbance measurement with shaking eeaeee Tabeware Name Standard 384 Sample Sample Sampie Sample Sampie Sampie Sample Walls X Y count 24 x 16 384 3 3 J aS a6 ET 55 Used Wells 187 Sample Sample Sample Sample Sampie Sample Labware Created Wednesday December 03 2003 3 3 a 5 56 a3 Labware Edited Wednesday December 03 2003 Sample Sample Sample Sample Sample Sample Notes None 70 70 3 10 4 10 5 10 8 10 7 10 8 Sample Sampie Sample Sample Sampie Sample Lot DefaultLot TT TT 174 116 TT 778 Lot Created Wednesday December 03 2003 Sample Sample Sample Sample Sampie Sample Sample Lot Optimized Wednesday December 03 2003 Notes None T10 TIZ Tis T15 T16 TAT Measurement Method 1 Set Temperature Sample Sample_ Sample Set Point C 20 210 Wait to Reach Temperature False Sample Measurement Method 2 Shake 5 7 Shake Type Linear Sample Sample Sample Sampie Sample Shake Intensity Medium 7 7 5 7 Shake Duration Sample Sample Sample Sampie Sample 5 Measurement Method 3 ABS 260 512 513 514 Measurement Technic Absorbance Sample Sampie Sample Measurement Method 4 ABS 340 6 17 6 1 6 7 Meceuemont Technic Abwobance sample Measurement Method 5 ABS 405 Measurement Technic Absorbance Sample Sample Sample Sample Matrix Legend Position 812 B13
55. To print protocol configuration information 1 In the Protocol Selection List select the protocol to print 2 From the toolbar choose Print the selected protocol OR From the Actions menu choose Print the selected protocol OR Right click on the selected protocol and choose Print the selected protocol from the menu Note Depending on how Print Settings are configured Print and or Print Preview may appear before the protocol configuration prints Refer to Section 1 4 2 2 Configuring Print Settings for more information about enabling and disabling Print and Print Preview Multimode Detection Software User s Manual 5 32 Defining Editing and Running Protocols 3 If Print and Print Preview are not enabled in Print Settings no additional configuration is required The protocol configuration and layout of samples on the plate prints OR If Print appears configure printing options as desired and choose OK OR If Print Preview appears use the toolbar controls to change the magnification layout view or page s displayed if desired Figure 5 24 Sprint preview Change page s displayed Change H rnational Laboratories International Laboratories layout view 7 Repon o1 Lab Repo 0149 Tine 13612 PM Pate Wednesday Apa 14 2004 SEXA Protocol Definition Absorbance 260 Protocol Definition Absorbance 260 Change view se magnification Row Wedari Ka i pH
56. View displays the zoomed view of the selected region To print the graph 1 From the File menu choose Print Print appears 2 Configure printing options as desired and choose OK to print the graph To close Graphic View From the File menu choose Close Beckman Coulter Inc Viewing Measurement Results es 6 3 Viewing Exported Measurement Results When a protocol has export and or data file options configured in Output Settings measurement results are exported after completing a protocol run Exported results are always opened viewed and evaluated in a compatible software application such as Microsoft Excel Multimode Detection Software only provides the ability to view results before they are exported Two file export and save options are available e Export to Microsoft Excel saves results in a format compatible with Microsoft Excel and automatically opens Excel when the protocol run is completed Refer to Section 6 3 1 Viewing Measurement Results Using Microsoft Excel for more information about viewing exported results in Excel Note Multimode Detection Software automatically determines the appropriate export method based on the version of Microsoft Office installed on the host computer XML xml files are exported when Office XP is installed When Office 2000 installed the measurement results are copied into a new spreadsheet which must be saved in Excel Versions of Excel prior to Office 2000 a
57. Viewing Kinetic and Scan Graphs 0 0 000s 6 6 6 2 3 1 Viewing Detailed Graphs of Individual Samples 6 7 6 3 Viewing Exported Measurement Results 00 0 eee 6 9 6 3 1 Viewing Measurement Results Using Microsoft Excel 6 9 6 3 1 1 Viewing Information About the Protocol 6 10 6 3 1 2 Viewing Measurement Results 04 6 11 6 4 Printing Measurement Results 0 0 00 c cece eens 6 13 A Appendix A Software Error Codes A l Troubleshooting Software Error Codes 0 0 0 0 0000 cece eee A 1 Beckman Coulter Inc List of Figures Figure 1 1 Multimode Detection Software main Window 0 cccccccceseceeeeeeseeeeceeeceeeeeeseeeaeees 1 2 Fig r 1 2 WSS oS GIG pctccscsusc ivcsveeubssaudlcnnsaneesivoncssssnpssneeevosssiuanbadee E EELE E iE 1 4 Figure 1 3 Installer Language Options ccccecccessceesseceeceeeeeseeeeseeceaeceeeeeeeeeseecaecneeeeeeenseees 1 6 Figure 1 4 Starting the software installation ccc cescseeseeeeeeeeceeeceeecaeeeseeeeeseeeaeceaecaeenaeeneeees 1 6 Fig t 1 5 Set p We ALCL es oincea vesaseten asp tanaipt asde e a iaa p Eae AAEN aA Ea EE 1 7 Figure 1 6 Selecting installation options cccecccecsseesseceseceeeeeeeeecseeceaeceeeeeseeeseecaeeneeseeeeeseees 1 7 Figure 1 7 Setup wizard repair or remove the software installation cceeceseeeeeeeeeeees 1 10 Figure 1 8 Multimode Detection Software
58. ad the imported protocol uses the same default methods and labware stored in the software Beckman Coulter Inc 6 1 Viewing Measurement Results 6 1 Overview Multimode Detection Software displays measurement results in a series of tabs within Run Protocol Figure 6 1 Raw data is displayed immediately after each column or row is read All measurements display sample status and raw data kinetic and scan measurements also display graphs for each sample Detection methods and measurement cycles performed in the protocol are displayed in the left pane of the tab results are displayed in the right pane The tabs may be viewed until Finish is chosen x Run Protocol Prepare to Run Run Protocol gt Click Finish to export data PITT TTT TTT TTT TTT Estimated Time 00 00 25 Measurement Time 00 00 28 Selected State Raw Data Graphs cycle to EFasezo H Cc 1 view ae Cycle 2 Cycle 3 Cycle 4 N sO o Ta e Cycl Cycl Inmo noop hoo HR OHO Oe ee ee Raw data Detection methods may be expanded and collapsed Finish Figure 6 1 Measurement results displayed after a protocol run Multimode Detection Software User s Manual 6 2 Viewing Measurement Results When Finish is chosen Run Protocol closes and results are exported and printed according to the file and printing options defined in the protocol refer to Sec
59. ams gt Beckman Coulter Inc gt Multimode Detection Software gt Multimode Detection Software Multimode Detection Software appears Figure 1 8 Note If Multimode Detection Software is not found in the Start menu the software may have been installed for a single user account on the system instead of all accounts Check with the site system administrator or login to the user account with permission to access the software Refer to Section 1 3 2 Using the Setup Program to Install the Software for more information about installing the software for a single or multiple user accounts B Multimode Detection Software Simulated File Actions Help Protocol Selection List SZ Protocols Application Created Last Edited Last Exe 260 Area Scan Basic Application 4 15 2004 4 26 2004 4 15 200 w Detection Methods Absorbance 260 Shaking Basic Application 4 15 2004 4 20 2004 4 15 200 Basic Abs Basic Application 4 26 2004 4 26 2004 4 26 200 Labware Basic ELISA 405 96 Basic Application 4 7 2004 4 7 2004 4 7 2004 Basic Endpoint Measurement Fluorescein 96 Basic Application 4 7 2004 4 7 2004 4 7 2004 Basic Kinetic Measurement Absorbance 340 96 Basic Application 4 7 2004 4 7 2004 4 7 2004 Fluorescence Polarization Basic Application 4 23 2004 4 23 2004 4 23 200 Multiwavelength Absorbance Basic Application 4 7 2004 4 7 2004 4 7 2004 Instruments Figure 1 8 Multimode Detection Software main screen Multimode Detection Software User
60. an be used only for measurements of the selected technique type s When techniques are selected the read only Installed field displays Yes When No is displayed no techniques are selected and the filter may not be used in any techniques gt DTX 880 Select Polarization only for filter positions where a polarization filter is installed Multimode Detection Software User s Manual 2 10 Setting Up and Controlling Instruments Beckman Coulter Inc 10 In Name enter a name for the selected filter Filter names default to the wavelength entered but may be renamed as desired 11 In Bandwidth enter the bandwidth in nanometers of the selected filter 12 Repeat steps 6 to 11 to configure additional filters on the slide 2 3 2 3 Removing Filter Slides If a filter slide is no longer used with an instrument it can be removed from Multimode Detection Software To remove a filter slide 1 Select the type of filter slide to remove Excitation or Emission The list of filter slides displays all slides of the selected type 2 Select the desired filter slide to remove from the list Filter Slide Properties displays information about the selected slide 3 Choose Remove Slide The selected filter slide is removed from the list 2 3 2 4 Exporting and Importing Filter Slides Information for all excitation and emission filter slides configured for the instrument can be exported to an XML file and imported to restore that
61. anges the slide must be configured To configure a filter slide 1 Select the type of filter slide to configure Excitation or Emission The list of filter slides displays all configured slides of the selected type 2 Select the desired filter slide to configure from the list Filter Slide Properties displays information about the selected slide Figure 2 3 3 In Slide ID enter the identification number printed on the slide 4 In Slide Name enter a name to identify the filter slide 5 In the list of filter slides click the to the left of the filter slide name to display the list of filters installed on the slide 6 Select a filter to configure Filter Properties for the selected filter is displayed on the right side of the screen Figure 2 4 as Instrument Settings Selected filter Basic Settings Filter Slides Ma ual Control Excitation Emissi Add Slide B Filter Properties Wavelength 535 Technique s Fluorescence R Slide Name 535 No Filter Bandwidth 25 ExpottSlides Properties of stale Yes osition selected filter Import Slides Name Name of the selected filter Cancel Apply Figure 2 4 Configuring filter properties 7 In Wavelength enter the wavelength of the filter 8 Click in the Technique s field and then click the down arrow to display a list of the available techniques 9 Select all techniques for which the filter applies The filter c
62. anguage highlighted Figure 1 13 2 Select the desired language 3 Choose Apply The system language changes to the language selected Beckman Coulter Inc Installing Setting Up and Using the Software 1 17 1 4 2 2 Configuring Print Settings Measurement results and protocol configurations may be printed Printing parameters such as headers and footers are configured in the Print Settings tab Figure 1 14 a5 Multimode Detection Software Connected Filter Slides Ex 2 Em 1 File Actions Help Protocols if Detection Methods Labware 4 Instruments Software Settings Software Settings Language Print Settings simulated Data Directory Settings l Print Header Line 1 international Laboratories A International Laboratories Line 2 Fluorescence Intensity Top Line 3 l Footer Footer 5 R international Laboratories Comment Print Options I Print Preview IV Show printer settings Figure 1 14 Configuring Print Settings To configure print settings l 2 Choose the Print Settings tab Print Settings appears Figure 1 14 In Print Header enter text for each header Line as desired Header lines may be left blank In Footer enter text for the Footer and Comment as desired The comment appears on printed pages below the footer The footer and comment may be left blank In Print Options select Print Preview to preview the page layout onscree
63. ar to rotate the graph horizontally Puinis sf Figure 6 5 Three dimensional area scan graph To rotate the graph e Use the vertical scroll bar to rotate the graph vertically e Use the horizontal scroll bar to rotate the graph horizontally e Double click on the graph to start and stop a continuous animated horizontal rotation of the graph To print the graph 1 From the File menu choose Print Print appears 2 Configure printing options as desired and choose OK to print the graph To close Graphic View From the File menu choose Close Multimode Detection Software User s Manual 6 8 Viewing Measurement Results 6 2 3 1 2 Viewing and Printing a Kinetic or Linear Scan Graph Kinetic and linear scan measurements display a two dimensional graph tracking the optical density OD measured in each cycle or at each point Figure 6 6 Two dimensional graphs support zooming in on selected regions and printing HE Graphic View File Selected region to zoom Well A12 3 Measurement Points Figure 6 6 Two dimensional kinetic graph with zoom region selected To zoom in on a region 1 Position the cursor at the desired starting point for the region then click and hold the mouse button down 2 Drag the mouse until the desired region is selected Figure 6 6 The selected region is highlighted in black 3 Release the mouse button Graphic
64. are User s Manual Creating and Editing Detection Methods Beckman Coulter Inc 3 2 3 3 Defining Fluorescence Intensity Top Method Parameters In a fluorescence intensity top method a light source above the plate is directed through an excitation filter which passes only the wavelength necessary to excite samples The resulting fluorescence passes through an emission filter that separates background light from the specific wavelength generated by samples This signal is read from above the plate by the photo multiplier tube 85 Method Editor Method Parameters Method Technique Method Parameters gt Method Name Fluorescence Intensity top Excitation Filter nm 535 SL 1 7 Emission Filter nm 595 SL 1 x Integration Time seconds 0 001 Date created Figure 3 6 Defining fluorescence intensity top method parameters To define fluorescence intensity top method parameters 1 Enter a Method Name Figure 3 6 2 Select the Excitation Filter nm 3 Select the Emission Filter nm Note The filters available are those installed on the slides loaded in the instrument and configured for fluorescence techniques in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 4 In Integration Time seconds enter the length of time in seconds the signal is collected from samples The integration time may be set within the range of 0 00005 to 3600 seconds 5 Choose Save to save th
65. asic absorbance measurement with shaking Cancel Figure 5 2 Defining a protocol name and entering notes about the protocol Multimode Detection Software User s Manual Defining Editing and Running Protocols To configure the general settings for a new protocol 1 In Protocol name enter a unique name for the protocol The name must be entered before the next configuration screen in the wizard can be accessed 2 In Notes enter a description of the new protocol if desired 3 Choose Next to select the measurement technique used in the protocol refer to Section 5 2 2 Selecting the Technique Type OR Choose Cancel to close Create Protocol without saving the new protocol 5 2 2 Selecting the Technique Type Use Technique Type to select the measurement technique performed by the protocol Figure 5 3 Only techniques supported by the instrument and for which detection methods have already been defined are available For more information about technique types refer to Chapter 3 Creating and Editing Detection Methods 8 create Protocol Absorbance 260 x Technique Type General Settings Technique Type gt Select technique below Click Next to proceed Click Back to go back Labware Selection Layout Settings Method Selection Output Settings Technique Type Luminescence Fluorescence Intensity Top Fluorescence Intensity Bottom Time Resolved Fluorescence Fluorescence Polarization Fi
66. bove ambient The maximum temperature that may be set is 45 C 113 F Note The temperature remains at the current setting until overridden by another Set Temperature preparation method by changing the temperature in Manual Control or by turning the instrument off and on To move the Set Temperature preparation method to a different location in the execution sequence in Method Selection select the method and choose Move the selected method forward or Move the selected method backward OR In Method Selection right click on the Set Temperature preparation method and choose Move Up or Move Down Multimode Detection Software User s Manual 5 18 Defining Editing and Running Protocols 5 2 6 Configuring Output Settings Use Output Settings to configure how measurement results are saved and or printed after a protocol run Figure 5 14 Options available include choosing to export results and automatically open them in Microsoft Excel following a protocol run choosing the file formats used to save measurement results and configuring which protocol information and measurement results are included in printouts An external software application such as a database may also be configured to execute after a protocol run xl Output Settings General Settings Labware Selection Select output options for Excel export and printer settings Layout Settings Excel export Method Selection Export to Microsoft Excel
67. ceisiasanccantshecussisaectaassaemealsnccaunnss 2 9 Figure 2 5 Instrument Settings Manual Control ssesssessessseeseeseesresseessesreeseeseesessseeseeseese 2 11 Figure 3 1 Accessing detection method functions sssssssssserssssssessseseesessesetsesseseesessersessesee 3 2 Figure 3 2 Selecting a Method Technique sisisssssicciavssseasssssncnseassseceiasssnsiuassanevaamssteasesssineaaarsacecants 3 3 Figure 3 3 Selecting an absorbance Method Type oa sasiastssassisessesiseessventanrasassassvancsaawnosssunntontsans 3 4 Figure 3 4 Defining absorbance measurement parameters s sessessssseseesesstsetsesstseesesseesessesee 3 6 Figure 3 5 Defining luminescence measurement parameters sssssssssssstsersrsetsesstseessssersessrsee 3 7 Figure 3 6 Defining fluorescence intensity top method parameters cccesseeteeeteeeeeeeeeees 3 8 Figure 3 7 Defining fluorescence intensity bottom method parametePS c cesceseesteereeereees 3 9 Figure 3 8 Defining fluorescence polarization method parameterS ceceeceseceteeneeereeeeees 3 10 Figure 3 9 Defining time resolved fluorescence method parameterS ccceseeeeseeneeereeeeees 3 11 Figure 3 10 Editing a fluorescence intensity top method e eee eeeereeeeeeeeeeeceeeceeeeeeeneeeeees 3 13 Multimode Detection Software User s Manual Figure 3 11 Confirming the deletion of a detection method cee eeeeeeseeeeeeeeceeeceeeeneeeneeenee
68. ched To set an exact temperature before starting a protocol run use Manual Control to set the temperature and check it at regular intervals until the desired temperature is reached refer to Section 2 3 3 1 Temperature Control 880 only Note Set Temperature preparation methods are useful for kinetic measurements intended to measure the effects of temperature changes on samples Defining Editing and Running Protocols 5 17 To configure a Set Temperature preparation method 1 In Method Selection choose the desired Set Temperature preparation method Method Properties for the selected method appear Figure 5 13 xl Method Selection General Settings Labware Selection Layout Settings Select from available methods below Click Next to proceed Click Back to go back Method Selection gt Output Settings 2 HA 3 Basic New Method Method Selection Method Properties Set Temperature AB5 260 Set Temperature Temperature in C to set chamber Cancel Figure 5 13 Configuring a Set Temperature preparation method In Set Temperature enter the desired microplate chamber temperature in degrees Celsius In protocols that perform measurements only at visible wavelengths gt 369 nm the minimum temperature that may be set is 3 C 5 4 F above ambient When the protocol performs measurements in the UV band using the deuterium lamp the minimum temperature is 4 C 7 2 F a
69. col select Optimize Read Height The Read Height Optimization Wizard appears Figure 5 17 a Read Height Optimization Wizard Select Detection Method Select a Detection Method ts Select a detection method to proceed The detection method should use the same technique that will be used in Prepare the Labware the target assay Click Next to proceed Optimize F Name Measurement technique Measurement type Created Last Edited Optimization Complete Fluorescein Top FluorescenceIntensityTop Monochromatic 1 28 2004 1 28 2004 PicoGreen FluorescenceIntensityTop Monochromatic 4 1 2004 4 9 2004 Rhodamin Top FluorescenceIntensityTop Monochromatic 1 28 2004 1 28 2004 Figure 5 17 Selecting the detection method used in the read height optimization 2 In Select Detection Method select the detection method used in the optimization For fluorescence top protocols choose the same detection method used in the protocol For fluorescence polarization and time resolved fluorescence protocols choose the detection method that is the closest match to that used in the protocol 3 Choose Next to select the well measured to perform the optimization Prepare Labware appears Figure 5 18 OR Choose Cancel to close the wizard without performing the optimization Multimode Detection Software User s Manual 5 24 Defining Editing and Running Protocols 4 Pipette liquid with a known maximum signal to a single well
70. configuration or share the filter slide configuration with another instrument Importing the filter slide configuration from an XML file replaces the current configuration for all filter slides with the configuration from the file To export all filter slides 1 Choose Export Slides Save As appears 2 In Save As select the desired directory and enter a file name 3 Choose Save Slide information is saved as an xml file with the specified path and file name To import all filter slides from a previously exported file 1 Choose Import Slides Open appears 2 In Open browse to and select the desired xml file to import 3 Choose Open The filter slides defined in the xml file are imported into the filter slide list and replace all existing filter slides Setting Up and Controlling Instruments 2 11 2 3 3 Manually Controlling the Instrument The Manual Control tab Figure 2 5 provides options to control functions of the connected instrument such as shaking microplates and ejecting or loading filter slides Manual Control is divided into five subsections Temperature Control 880 only Section 2 3 3 1 e Shake Control Section 2 3 3 2 e Plate Control Section 2 3 3 3 Excitation and Emission Filter Slide Control Section 2 3 3 4 5 instrument Settings ae x Basic Settings Filter Slides Manual Control Temperature Control Plate Control Actual fo Eject Load Set Point fo
71. configured in Labware Information refer to Section 4 2 1 Defining Labware Information Filter wavelength or filter type in current detection method is not installed Check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides Filter wavelength in command is not defined in any Slide Check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides Currently inserted slide code does not match set slide code Reinsert slide Make sure the correct filter slides are installed in the instrument No filter slide detected Make sure filter slides are installed Currently inserted filter slide code is invalid Make sure the correct filter slides are installed in the instrument Currently inserted filter slide contains no valid defined filters Make sure the correct filter slides are installed in the instrument and check filter parameters configured in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 51 Temperature setpoint exceeds 45 C 113 F Check the temperature set in Manual Control and or the protocol Set a desired temperature less than 45 C if necessary refer to Section 2 3 3 1 Temperature Control 880 only Currently inserted LED is not defined Contact a Beckman Coulter Service Engineer LED causing
72. ction Guidelines When creating labware select only measurement techniques compatible with the microplate being defined Each measurement technique requires labware of a specific color and or material be used Table 4 1 provides general labware color and material guidelines for each measurement technique Along with these basic guidelines always select microplates with a surface treatment suitable for the desired application and follow any additional guidelines provided by the plate manufacturer Table 4 1 General Microplate selection guidelines Measurement Supported Plate Color Additional Considerations Technique Absorbance clear white with clear bottom or black with clear bottom Clear polystyrene or film plates with transparent bottoms are suitable Polypropylene or PVC plates do not provide sufficient optical quality Luminescence Glow solid black or solid Black plates are recommended unless the signal Type white is weak enough to require the higher sensitivity of white plates However with strong signals white plates may produce crosstalk Fluorescence Intensity solid black N A Top Fluorescence Intensity black with clear N A Bottom bottom Fluorescence solid black N A Polarization Time Resolved solid white N A Fluorescence 4 2 2 Configuring Offsets and Well Dimensions for the Default Labware Lot Use Labware Lots to define row and column offsets
73. d For example Figure 5 12 shows a protocol where the microplate is shaken before absorbance measurements are performed at three wavelengths Note A Shake preparation method only shakes the plate once for the configured duration and intensity To perform interval shaking between each cycle in a kinetic measurement shaking must be configured in Method Properties for the desired detection method refer to Section 5 2 5 1 Configuring a Method in Basic Mode To configure a Shake preparation method 1 In Method Selection choose the desired Shake preparation method Method Properties for the selected method appear Figure 5 12 Eb create Protocol Absorbance 260 NNNE Method Selection General Settings Technique Type Select from available methods below Click Next to proceed Click Back to go back Labware Selection Layout Settings Basic New Method Output Settings Method Selection Method Properties Shake Intensity Medium ABS 260 Shake Interval 5 ABS 340 Shake Mode Linear ABS 405 Shake Intensity The intensity of the shaking operation Cancel Figure 5 12 Configuring a Shake preparation method CAUTION Shake low density plates such as 6 or 48 well plates at low AN speed only Shaking low density plates at higher speeds may cause liquid in wells to spill 2 In Shake Intensity select the desired intensity of shaking Low Medium or High Multimode Detection Software User s Manual
74. ding technique type labware type detection method s and preparation methods such as shaking Protocols are stored in the Protocol Selection List Figure 5 1 which provides access to all protocol functions e Creating Protocols Section 5 2 Running Protocols Section 5 3 Editing Protocols Section 5 4 e Copying Protocols Section 5 5 Deleting Protocols Section 5 6 e Printing Protocol Configuration Information Section 5 7 Exporting and Importing Protocols Section 5 8 Multimode Detection Software User s Manual Defining Editing and Running Protocols To select protocols and access protocol functions From the navigation pane choose Protocols The Protocol Selection List appears Figure 5 1 5 Multimode Detection Software Connected Filter Slides Ex 2 Em 1 File Actions Help Protocol 19 15 3 e S functions Protocol Selection e Protocols Name Application Created Last Edited Last Executed Er Absorbance 340 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 Detection Methods Fluorescein Top 384 Basic Application 1 29 2004 1 29 2004 1 29 2004 Luminecsence 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 Labware Rhodamin Bottom 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 TRF Europium 384 Basic Application 1 29 2004 3 17 2004 1 29 2004 Instruments Software Settings Protocols Figure
75. e The run options available are different for each mode For more information refer to Running a Protocol on an Instrument Section 5 3 1 Running a Protocol When Simulation Mode is Enabled Section 5 3 2 5 3 1 Running a Protocol on an Instrument Running a protocol on an instrument performs measurements on samples and outputs results data following the parameters configured in the protocol To run a protocol on an instrument 1 In the Protocol Selection List select the protocol to run 2 From the toolbar choose Run the selected protocol OR From the Actions menu choose Run the selected protocol OR Right click on the selected protocol and choose Run the selected protocol from the menu Prepare to Run Protocol appears Figure 5 16 5 Run Protocol Fluorescein Top 384 x Prepare to Run Protocol Prepare to Run Proto gt Run Protocol Click Next to run protocol Eject Plate Carrier Optimize Read Height Only available for the DTX 880 Close Plate Carrier Landscape Optimized Portrait Select Plate Orientation C Opposka Lendscene Opposite Portrait Run the selected protocol C Default Optimize Read Height YS C Maximum C Set Value Figure 5 16 Preparing to run a protocol on an instrument 3 Use Eject Plate Carrier and Close Plate Carrier to load the microplate into the instrument if necessary Multimode Detection Software User s Manual
76. e lower two wells and edit the dimensions if desired In Row distance verify the distance between rows and edit the dimension if desired Verify the x and y offsets for the upper two wells and edit the dimensions if desired If necessary click the next to Labware Lot Name to display the default name assigned to the new labware lot Enter a new Lot ID Name if desired Multimode Detection Software User s Manual 4 18 Creating and Editing Labware 8 Choose Save to save the optimization data and create the new labware lot Note To use the optimized lot in a measurement protocol open the labware for editing and select the new Labware Lot refer to Section 4 3 2 Selecting and Editing Labware Lots OR Choose Back to redefine well centers graphically refer to Section 4 6 4 Selecting the Centers of the Four Corner Wells OR Choose Cancel to close Optimizing Labware without completing the optimization Beckman Coulter Inc Creating and Editing Labware 4 19 4 7 Exporting and Importing Labware User defined labware saved in the Labware Selection List can be exported to an XML file and imported later to restore that configuration or share it with a copy of Multimode Detection Software installed on another computer Exporting default labware included with the software installation is not necessary Since default labware may not be edited deleted or overwritten it is available to user
77. e new fluorescence intensity top detection method The new method appears in the Detection Method Selection List OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without saving the new detection method Creating and Editing Detection Methods 3 9 3 2 3 4 Defining Fluorescence Intensity Bottom Method Parameters 880 only In a fluorescence intensity bottom method a light source below the plate is directed through an excitation filter which passes only the wavelength necessary to excite samples The resulting fluorescence passes through an emission filter that separates background light from the specific wavelength generated by samples This signal is read from below the plate by the photo multiplier tube 8S Method Editor Method Parameters Method Technique Method Parameters gt Method Name Fluorescence Intensity bottom Excitation Filter nm 535 SL 1 z Emission Filter nm 5955L1 z Integration Time seconds 0 001 Date created Figure 3 7 Defining fluorescence intensity bottom method parameters To define fluorescence intensity bottom method parameters 1 Enter a Method Name Figure 3 7 2 Select the Excitation Filter nm 3 Select the Emission Filter nm Note The filters available are those installed on the slides loaded in the instrument and configured for fluorescence techniques in
78. e protocol Figure 5 6 Create Protocol Absorbance 260 E General Settings Technique Type Layout Settings Layout Selection Settings Reading Direction Settings Labware Selection Layout Settings gt Method Selection Output Settings Select the reading direction of the plate below To read a plate row by row select Read by row To read a plate column by column select Read by column To read well by well select Read by well The well by well reading method reads an entire well before moving on to the next well which can be useful for scan and fast kinetic measurements Read by row E E Read by Column Read by column Available for all measurement techniques except absorbance Read by well Sa Cancel Figure 5 6 Selecting how wells on the plate are read 4 Select the desired reading direction Read by row Reads plates row by row Read by column Reads plates column by column Available for all measurement techniques except absorbance Read by well Reads each well individually before reading the next well which is useful for kinetic and scan measurements Multimode Detection Software User s Manual 5 8 Defining Editing and Running Protocols 5 Choose Next to configure detection and preparation methods refer to Section 5 2 5 Configuring Detection and Preparation Methods OR Choose Back to select a different labware type refer to Section 5 2 3 Se
79. e technique selected for the protocol are available refer to Section 5 2 2 Selecting the Technique Type OR Choose New Method to create a new detection method using the currently selected technique type Refer to Chapter 3 Creating and Editing Detection Methods for more information about creating detection methods To configure a kinetic measurement in Method Properties click in Kinetic and choose Yes Kinetic measurements read wells several times at specified intervals Note Ifa kinetic measurement not desired proceed to step 6 In Kinetic Cycles enter the number of measurement cycles to be performed Kinetic measurements may be set to perform to 100 cycles Multimode Detection Software User s Manual 5 10 Defining Editing and Running Protocols 5 In Kinetic Interval enter the length of time in seconds between each measurement of the same well Note The minimum kinetic interval is populated automatically in Kinetic Interval and is determined by the labware type and layout settings configured in the protocol The maximum interval between each measurement cycle is 65 535 seconds 6 To shake the microplate before performing the measurement click in Shake and choose Yes In kinetic measurements the plate is shaken for the specified intensity and interval before each measurement cycle Note If shaking is not desired proceed to step 10 CAUTION Shake low density plates such as 6
80. e time Note Refer to the DTX 800 880 Multimode Detectors User s Manual Section 1 3 2 Exchanging Filter Slides and Individual Filters for information on installing filters and filter slides H Instrument Settings e x Basic Settings Filter Slides Manual Control Excitation C Emission Add Slide a Filter Slide Properties 535 Slide ID 1 485 Slide Name 1 Remove Slide 495 No Filter 370 Export Slides No Filter Import Slides Slide ID Identification number of the selected filter slide Figure 2 3 Instrument Settings Filter Slides 2 3 2 1 Adding Filter Slides When a new filter slide is used with the instrument for the first time it must be added so Multimode Detection Software can identify the slide and filter configuration To add filter slides 1 Select the type of filter slide to add Excitation or Emission The list of filter slides displays all slides of the selected type currently stored in memory 2 Choose Add Slide A new filter slide is added to the list of filter slides 3 Configure the slide following the steps in Section 2 3 2 2 Configuring Filter Slides Beckman Coulter Inc Setting Up and Controlling Instruments 2 9 2 3 2 2 Configuring Filter Slides The filter slide configuration includes a name and ID for the slide and information about the filters installed on the slide When a new slide is added or the filter configuration on a slide ch
81. ection Software the instrument must be set up and configured All configured instruments are listed separately in the Instrument Selection List The Instrument Selection List contains quick access to control common instrument functions such as loading and ejecting the plate carrier and to configure instrument settings Use the Instrument Selection List for e Controlling Instrument Functions Section 2 2 e Configuring Instrument Settings Section 2 3 Multimode Detection Software User s Manual Setting Up and Controlling Instruments To configure and manually control instruments From the navigation pane choose Instrument The Instrument Selection List appears Figure 2 1 as Multimode Detection Software Simulated File Actions Help Instrument Selection List Name Serial Measurement techniques Multimode Reader 0000 Absorbance Luminescence Fluorescence Intensity Top Fluorescence Intensity Bottom Fluorescence F by Detection Methods Labware X 55 Software Settings Figure 2 1 Instrument Selection List All instruments that have been connected to the host computer and configured in the software are displayed in the Instrument Selection List When an instrument not currently connected to the computer is selected the software automatically enters simulation mode refer to Section 2 2 5 Enabling Simulation Mode This allows protocols detection methods and labware to be defined
82. ed protocol from the menu A copy of the selected protocol appears in the Protocol Selection List Note The default name format for copied protocols is Copy of OriginalName To change the name open the protocol for editing and enter the new protocol name refer to Section 5 4 Editing Protocols Multimode Detection Software User s Manual 5 30 Defining Editing and Running Protocols 5 6 Deleting Protocols User defined protocols that are no longer used to perform measurements may be deleted from the Protocol Selection List Default protocols included with the software installation may not be deleted To delete a user defined protocol 1 In the Protocol Selection List select the protocol to delete 2 From the toolbar choose Delete the selected protocol OR From the Actions menu choose Delete the selected protocol OR Right click on the selected protocol and choose Delete the selected protocol from the menu Message appears Figure 5 23 mesae 2 Are you sure you want to delete the protocol 260 Area Scan e Figure 5 23 Confirming the deletion of a protocol 3 Choose Yes to delete the selected protocol OR Choose No to cancel the deletion Beckman Coulter Inc Defining Editing and Running Protocols 5 31 5 7 Printing Protocol Configuration Information Information about the protocol configuration and sample layout may be printed separately from measurement results
83. ee 5 17 Figure 5 14 Configuring output SCtIN GS 5ccccsccsssccascessccsssesanessantsessnensscssteniaespenssseeatenssnnsvnesines 5 18 Figure 5 15 Configuring an external software application in Output Settings 5 20 Figure 5 16 Preparing to run a protocol on an instruMent cece eeeeeesceeeeceeeceseecsaeeeteenees 5 21 Figure 5 17 Selecting the detection method used in the read height optimization 5 23 Figure 5 18 Selecting the well read in the read height optimization eceeceeeeeteeneereeeeees 5 24 Figure 5 19 Read height optimization in Progress ccsiassssanssaencccsssesndenancondansasnndenssutegananbenganss 5 25 Figure 5 20 Read height optimization completed eecceececeseceecneeeseeeeeeeeceaeceeeeeeeseeeeees 5 25 Figure 5 21 Preparing to run a protocol in simulation MOde eeeeseeeeeeeeceeecneeeeeeneeeeeeenees 5 26 Figure 5 22 Editing an absorbance protocol wssisssscsvsscsaccnnssnevnassesaetannsscastasssdeviasasei nvaupdevnduncaas 5 28 Figure 5 23 Confirming the deletion of a protocol 0 0 eceecceseeceeeceeeneeeseeeeeeeeceaeceeeeeeeneeeeees 5 30 Figure 5 24 Previewing a protocol configuration and sample layout printout 6 5 32 Figure 5 25 Choosing the folder where an exported protocol will be saved ee ceeeeeeeees 5 33 Figure 6 1 Measurement results displayed after a protocol run ecceeceeseeseceeeeeeeeeeseeeeeeens 6 1 Figure 6 2 State displayed for an area Sca
84. efer to Section 4 6 1 Selecting the Detection Method OR Choose Cancel to close Optimizing Labware without performing the optimization Beckman Coulter Inc Creating and Editing Labware 4 15 4 6 3 Performing the Optimization Read Optimization in Progress displays the status of the optimization read and provides the ability to cancel the optimization in progress Figure 4 9 The optimization read requires several minutes to complete ioj xi Optimization in Progress S Select a Detection Method Wells are currently being read Please wait Prepare the Labware Select Center of Left Top Well Select Center of Left Bottom Well Select Center of Right Top Well Select Center of Right Bottom Well Performing optimization scan Verify Well Centers Estimated Time 00 01 13 Measurement Time 00 00 06 Press Stop Optimization to cancel labware ETES optimization PSP Cancel Figure 4 9 Labware optimization in progress During the optimization read Choose Stop Optimization to cancel the optimization process and close Optimizing Labware without saving the optimization data When the optimization read is complete Choose Next to select the centers of the four corner wells refer to Section 4 6 4 Selecting the Centers of the Four Corner Wells OR Choose Cancel to close Optimizing Labware without performing the optimization Multimode Detection Software User s Manual 4 16 Creatin
85. efer to Section 6 2 3 Viewing Kinetic and Scan Graphs 6 2 1 Viewing State State displays which samples were measured successfully and which were not because of errors during the measurement Figure 6 2 e OK sample measured successfully e Error sample not measured because an instrument error occurred e Overflow no results available because the value measured exceeds the upper measurement limit e Incorrect sample was measured but is incorrect because an instrument error occurred e Unused sample not selected for measurement in the protocol Z Run Protocol Absorbance 260 Shaking p x Run Protocol Prepare to R repare co Sun Click Finish to export data Detection DANMNINTANTAMNCANANCANNNA N meth od s Estimated Time 00 00 12 Measurement Time 00 00 14 State Raw Data Graphs AB5 260 Cycle 1 Hia L1 2 1 3 ae 1 5 16 L147 Measurement p e 1 9 cycle or L110 sample ae 1 12 1 13 1 15 1 16 LI 1 18 1 19 Figure 6 2 State displayed for an area scan measurement Multimode Detection Software User s Manual 6 4 Viewing Measurement Results To view State of samples or measurement points 1 If necessary in the left panel of the tab click the next to the desired detection method to expand the view and access measurement cycles Figure 6 2 2 For absorbance luminescence and fluorescence measurements select
86. ell microplate in the plate carrier To manually perform a shaking operation CAUTION Shake low density plates such as 6 or 48 well plates at low AN speed only Shaking low density plates at higher speeds may cause liquid in wells to spill 1 In Mode select the desired shaking mode e Linear shakes from side to side e Orbital shakes in a circular pattern e Squared shakes in a square pattern moving at right angles 2 In Intensity select the desired shaking intensity Low Medium or High 3 In Duration enter the length of time to shake in seconds 4 Choose Shake The instrument shakes the microplate according to the configured settings 2 3 3 3 Plate Control Plate Control provides options to eject or load the plate carrier It also features an option to sense that a microplate is in the plate carrier before starting a measurement To manually control the plate carrier e Choose Eject to extend the plate carrier outside the instrument e Choose Load to retract the plate carrier inside the instrument e Select Check if plate is inserted before each read to sense if a microplate is in the plate carrier before starting each measurement 2 3 3 4 Excitation and Emission Filter Slide Control Excitation Filter Slide Control and Emission Filter Slide Control are used to manually eject or load the excitation or emission filter slides To manually eject or load the excitation or emission filter slide e Choose Eject
87. er s Manual 1 4 Installing Setting Up and Using the Software 1 2 3 Accessing Online Help Multimode Detection Software contains a detailed online help system that covers defining and editing labware detection methods and protocols performing measurements and exporting measurement results The online help is context sensitive which provides instant access to help for the active screen To access online help Press F1 at any time to display online help for the active screen Figure 1 2 OR From the Help menu choose Help to display the table of contents Multimode Detection Software Help ae gt Hide Back Forward Stop Refresh Contents Index Search Glossary Setting Up and Using the Software amp Setting Up and Manually Controlling Ins Creating and Editing Labware Creating and Editing Detection Method Q Defining Editing and Running Protocol 2 Overview QG Creating Protocols B Configuring General Settings Eit Selecting the Technique Type 2 Selecting Labware 12 Configuring Labware Layout Se Selecting Methods F Configuring Output Settings Running Protocols B Editing Protocols 2 Copying Protocols 2 Deleting Protocols 2 Printing Protocols e Viewing Measurement Results OHARA Beckman Coulter Inc You are here Defining Editing and Running Protocols gt Creating Protocols gt Selecting the Techn
88. eserved No part of this publication may be reproduced distributed or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise or stored in a database or retrieval system without the prior written permission of Beckman Coulter Inc Beckman Coulter Inc Warranty and Returned Goods Requirements All standard Beckman Coulter Inc policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Company warrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such one year period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows a Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list
89. ethod Type choose the desired method Monochromatic or Bichromatic 2 Choose Next to define Method Parameters refer to Section 3 2 3 Defining Method Parameters OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without defining a new detection method 3 2 3 Defining Method Parameters Parameters such as filters and integration time are defined in Method Parameters The parameters available for configuration depend on the technique selected in Method Technique refer to Section 3 2 1 Selecting a Method Technique Use Method Parameters for Defining Absorbance Method Parameters Section 3 2 3 1 Defining Luminescence Method Parameters Section 3 2 3 2 Defining Fluorescence Intensity Top Method Parameters Section 3 2 3 3 Defining Fluorescence Intensity Bottom Method Parameters 880 only Section 3 2 3 4 Defining Fluorescence Polarization Method Parameters 880 only Section 3 2 3 5 Defining Time Resolved Fluorescence Method Parameters 880 only Section 3 2 3 6 Multimode Detection Software User s Manual 3 6 Creating and Editing Detection Methods 3 2 3 1 Defining Absorbance Method Parameters A monochromatic absorbance method performs an absorbance measurement at a single wavelength A bichromatic method performs a second measurement at a refere
90. ethod Type Supported techniques absorbance Method Parameters Figure 3 2 Selecting a Method Technique Multimode Detection Software User s Manual 3 4 Creating and Editing Detection Methods To select a method technique 1 In Supported techniques choose the desired detection method 2 Ifdefining an absorbance method choose Next to select the Method Type refer to Section 3 2 2 Selecting the Type of Absorbance Method OR If defining a luminescence or fluorescence method choose Next to define Method Parameters refer to Section 3 2 3 Defining Method Parameters OR Choose Cancel to close the Method Editor without defining a new detection method 3 2 2 Selecting the Type of Absorbance Method When defining an absorbance detection method use Method Type to select whether a monochromatic or bichromatic method will be defined Figure 3 3 Monochromatic methods perform a single wavelength measurement Bichromatic methods perform a second measurement at a reference wavelength which is subtracted from the first to calculate the final result Note Method Type appears only when defining absorbance methods FjMethod Editor Method Type Method Technique MethodType gt Method Type Monochromatic x Method Parameters Cancel Figure 3 3 Selecting an absorbance Method Type Beckman Coulter Inc Creating and Editing Detection Methods 3 5 To select a method type 1 In M
91. f measurement cycles performed or points measured Protocol Information Prints the name of the protocol underneath the printout header Labware Information Prints information about the labware used including plate type number of wells measured lot name and the date that the lot was optimized General Information Prints general information about the protocol including when the protocol was created or last edited and performed Plate Graph Prints graphs of kinetic and scan measurement results Only available for configuration when a kinetic area scan or linear scan measurement is configured in the protocol Method Information Prints information about the detection method s used in the protocol Multimode Detection Software User s Manual 5 20 Defining Editing and Running Protocols 5 2 6 1 Configuring a Program to Run after Protocol Executes Output Settings can be configured to open an external software application such as a custom data logger or database after completing a protocol run If the selected application supports entering commands using a command line interface a specific command for the application may also be configured To configure a program to run after a protocol is executed 1 In Output Settings choose Execute a program after protocol executes Execute Program expands to show the fields that may be configured Figure 5 15 EE Output Settings General Setti
92. f the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement Representatives and warranties made by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company Parts replaced during the warranty period are warranted to the end of the instrument warranty Note Performance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used Multimode Detection Software User s Manual Safety Information All Warnings and Cautions in this document include an exclamation point a lightning bolt or a light burst symbol framed within a triangle Please pay special attention to the specific safety information associated with these symbols WARNING If the equipment is used in a manner not specified by AN Beckman Coulter Inc the protection provided by the equipment may be impaired Warning and Caution Definitions The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing is attempted When this symbol is displayed in this manual pay special attention to the specific safety information associated wit
93. file is not permitted by the software To export a protocol 1 In the Protocol Selection List select the protocol to export 2 From the File menu choose Export gt Protocol Browse for Folder appears Figure 5 25 My Documents p This Computer a My Network Places w Recycle Bin O Exported Protocols Make New Folder Figure 5 25 Choosing the folder where an exported protocol will be saved 3 In Browse for Folder browse to the folder where the exported protocol will be saved OR Choose Make New Folder to create a new folder where the exported protocol will be saved 4 Choose OK to export the protocol The exported protocol is saved using the default file name format Protocol_ProtocolName xml In order to import the file at a later date the filename must not be changed OR Choose Cancel to stop the operation without exporting the protocol Multimode Detection Software User s Manual 5 34 Defining Editing and Running Protocols To import a protocol from an exported XML file 1 From the File menu choose Import gt Protocol Open appears 2 In Open browse to and select the desired XML file to import 3 Choose Open The protocol as well as detection method s and labware used in the protocol are imported Any default detection method s or labware used in the protocol are not imported because default methods and labware may not be edited deleted or overwritten Inste
94. g and Editing Labware 4 6 4 Selecting the Centers of the Four Corner Wells Use Select Center to precisely define the centers of the corner wells read in the optimization Figure 4 10 Select Center displays an image of the well generated by the absorbance measurement Well centers are defined graphically by dragging cross hairs to the position visually identified as the center Select Center is performed for each comer well individually 101 x Select Center of Left Top Well Select the center of the well graphically below Click next to proceed or back to optimize the labware again Select a Detection Method Prepare the Labware Optimize Select Center of Left Bottom Well Select Center of Right Top Well Select Center of Right Bottom Well Verify Well Centers Figure 4 10 Selecting the well center To define the centers of the wells 1 Place the cursor in the well image then click and drag the cross hairs to the desired center of the well 2 Choose Next to define the center of the next well read When all four well centers are defined Verify Well Centers appears refer to Section 4 6 5 Verifving Well Centers OR Choose Cancel to close Optimizing Labware without completing the optimization Beckman Coulter Inc Creating and Editing Labware 4 17 4 6 5 Verifying Well Centers Use Verify Well Centers to verify that the x and y offsets and distances between rows and columns are correct Figure 4 1
95. gure 5 3 Selecting the measurement technique for a DTX 880 Beckman Coulter Inc Defining Editing and Running Protocols 5 5 To select a technique type 1 In Technique Type choose the desired technique 2 Choose Next to select the type of labware used in the protocol refer to Section 5 2 3 Selecting the Labware Type Used in the Protocol OR Choose Back to configure General Settings for the protocol refer to Section 5 2 1 Configuring General Settings OR Choose Cancel to close Create Protocol without saving the new protocol 5 2 3 Selecting the Labware Type Used in the Protocol Use Labware Selection to select the type of labware used in the protocol Figure 5 4 Labware must be configured prior to configuring the protocol Only labware configured for the selected technique type is available Labware cannot be edited once it is used in a protocol Refer to Chapter 4 Creating and Editing Labware for detailed information about creating and configuring labware E Labware Selection General Settings Technique Type Select labware in list below Click Next to proceed Click Back to go back Labware Selection Layout Settings Method Selection Output Settings Type of Labware Name Number of Wells Greiner 96 Cone PS 96 Greiner 96 Flat 96 Greiner 96 Round PP 96 Greiner 96 Round PS 96 Greiner 96 Thermal Cycler 96 Greiner Flat 384 Square 384 Greiner Shallow 384 Round 384 LIL Shallow 384 Round 38
96. h the symbol WARNING A WARNING calls attention to a condition or possible situation that could cause injury to the operator CAUTION A CAUTION calls attention to a condition or possible situation that could damage or destroy the product or the operator s work Multimode Detection Software User s Manual vi Safety Information Warnings and Cautions Found in this Manual A A A Beckman Coulter Inc Please read and observe all cautions and instructions Remember the most important key to safety is to use Multimode Detection Software with care The WARNINGs and CAUTIONs found within this document are listed below WARNING If the equipment is used in a manner not specified by Beckman Coulter Inc the protection provided by the equipment may be impaired CAUTION Shake low density plates such as 6 or 48 well plates at low speed only Shaking low density plates at higher speeds may cause liquid in wells to spill CAUTION The plate height configured must not be less than that of the actual plate Doing so may cause the DTX 880 optics transport to collide with the plate during a Read Height Optimization vii Table of Contents Safety Information 4 66 iewdheidiunewsediecdekewieaw ered iwedheddanneasedd enews ae v Tableof Contents asiscce cid cen ae 08 COWS ES e sd e ERE WE ROR wa E E E Vii List Of FIGUQeS 6 2524 vdsee Dede dS EEE ENS ERE Ed ER OREO eee Eee ede oes xi 1 Installing Setting Up and Using
97. he current user account has Administrator privileges Accounts with Standard or Restricted access are not permitted to run Setup Contact the site system administrator for more information about account privileges 3 Insert the Multimode Detection Software installation CD into the CD ROM drive After a few seconds Language Options appears Note If Language Options does not appear automatically within a few seconds of inserting the CD into the drive use Windows Explorer to browse to the CD ROM drive and run MultimodeDetectionSoftwarelnstall exe 4 If repairing software installed on a German language version of Windows choose German Language Operating System This ensures that all software screens are displayed correctly when German is set as the system language used in the Multimode Detection Software interface 5 In Language Options choose Install to start the installation Multimode Detection Software Installation appears 6 Choose OK to continue The Multimode Detection Software setup wizard appears 7 Choose Next to continue Options to repair or remove the software appear Figure 1 7 Multimode Detection Software User s Manual 1 10 Installing Setting Up and Using the Software Beckman Coulter Inc Multimode Detection Software Welcome to the Multimode Detection Software Setup Wizard Select whether you want to repair or remove Multimode Detection Software Repair Multimode Detection Softwa
98. he selected protocol from the menu OR Double click on the selected protocol Edit Protocol appears Figure 5 22 SS Edit Protocol Absorbance 260 General Settings General Settings gt Labware Selection Layout Settings Method Selection Output Settings Please enter a name and notes for this protocol Click Next to proceed Protocol name Absorbance 260 Date created Wednesday April 14 2004 Date edited Wednesday April 14 2004 Date last run Wednesday April 14 2004 Notes Basic absorbance measurement with shaking Figure 5 22 Editing an absorbance protocol 3 Edit the parameters in each Edit Protocol screen as desired Beckman Coulter Inc Note Refer to Section 5 2 Creating Protocols for detailed information about configuring protocol parameters Choose Save to close Edit Protocol and save the changes OR Choose Cancel to close Edit Protocol without saving changes Defining Editing and Running Protocols 5 29 5 5 Copying Protocols Copies may be made of existing protocols After a copy has been created it may be used as a template for a new protocol using the same technique To make a copy of a protocol 1 In the Protocol Selection List select the protocol to copy 2 From the toolbar choose Make a copy of the selected protocol OR From the Actions menu choose Make a copy of the selected protocol OR Right click on the selected protocol and choose Make a copy of the select
99. hoose Cancel to close the Labware Editor without saving changes Creating and Editing Labware 4 9 4 3 2 Selecting and Editing Labware Lots Use Labware Lots to select the active lot and or edit and save changes made in the Labware Editor Figure 4 5 Lots can be selected and edited for all labware including labware used in measurement protocols Note Labware Lots are created by optimizing labware refer to Section 4 6 Optimizing Labware 85 Labware Editor SemiNewLabware xi Labware Lots Labware Information Choose the lot Labware Lots gt DefaultLot X _ to use or edit E Labware Lot Info a Current labware lot True Landscape d with Absorbance Property B Labware Lot Measurements g rid Lower left x offset 1 435 Lower left y offset 1 135 Lower right x offset 1 435 CAUSE 1 135 Upper left x offset 1 435 Upper left y offset 1 135 Upper right x offset 1 435 Upper right y offset 1 135 E well Measurements Column distance 0 9 Row distance 0 9 x Lower right y offset Offset From lower right corner of labware to First row cm Figure 4 5 Configuring offsets and well dimensions in Labware Lots To select and edit lots 1 From the pull down menu choose the lot to use or edit The default lot created when the labware was defined and all lots configured using Optimizing Labware are available refer to Section 4 6 Optimizing Labware Click in
100. ines if this method uses a scan read Cancel Figure 5 11 Configuring a method in advanced mode Multimode Detection Software User s Manual Defining Editing and Running Protocols E Beckman Coulter Inc Add a detection method by choosing Add a method to this protocol then selecting the desired Detection Method from the menu that appears OR Right click in Method Selection and choose Add Method gt Detection Methods gt and the desired detection method from the menu that appears OR Choose New Method to create a new detection method Refer to Chapter 3 Creating and Editing Detection Methods for detailed information about creating detection methods Configure Method Properties as desired Refer to Section 5 2 5 1 Configuring a Method in Basic Mode for more information about configuring method property parameters Add and configure additional detection methods by repeating steps 2 and 3 for each method desired Add a preparation method by choosing Add a method to this protocol then selecting Preparation Methods gt Shake or Set Temperature from the menu that appears OR Right click in Method Selection and choose Add Method gt Preparation Methods gt Shake or Set Temperature from the menu that appears gt DTX 880 Set Temperature is available only when controlling this instrument Configure the preparation method as desired Refer to Section 5 2 5 2 1 Configu
101. ings OR Choose Cancel to close Create Protocol without saving the new protocol 5 2 5 2 Configuring a Method in Advanced Mode Configuring methods in advanced mode includes selecting detection and preparation methods arranging the execution sequence of methods and optionally configuring properties for a kinetic or scan measurement Protocols that require multiple measurements at different wavelengths such as multiwavelength absorbance or fluorescence resonance energy transfer FRET measurements must be configured in advanced mode To select and configure a method in advanced mode 1 In Method Selection choose Advanced to toggle from basic mode if necessary Advanced mode configuration parameters appear Figure 5 11 Refer to Section 5 2 5 1 Configuring a Method in Basic Mode to configure a protocol that requires only one detection method and no preparation methods f create Protocol Absorbance 260 Method Selection General Settings Technique Type Method Selection kacktogo Method Properties Labware Selection Add or remove methods and change Configure a kinetic or Layout Settings the order in which they are executed scan measurement Output Settings Method Selection Method Properties Adds a 4BS 260 Kinetic method Len et Scan Moves the selected method forward or backward in the execution sequence Deletes the selected method Scan Determ
102. ion 2 2 5 Enabling Simulation Mode To run a protocol in simulation mode 1 In the Protocol Selection List select the protocol to run 2 From the toolbar choose Run the selected protocol OR From the Actions menu choose Run the selected protocol OR Right click on the selected protocol and choose Run the selected protocol from the menu Prepare to Run Protocol appears Figure 5 21 A Run Protocol Absorbance 260 Prepare to Run Protocol Prepare to Run Proto gt Run Protocol Click Next to run protocol I Check to generate random data Use this Data Simulation File for this read c program files beckman coulter multimode detection software templates ABS96Simulated dat Run the selected protocol Directory path of simulated data file Figure 5 21 Preparing to run a protocol in simulation mode 3 Select Check to generate random data to run the protocol using random data generated by the software if desired Beckman Coulter Inc Defining Editing and Running Protocols 5 27 4 In Use this Data Simulation file for this read e Leave the directory path of the simulated data path as configured to use the data file selected in Software Settings refer to Section 1 4 2 3 Choosing Simulated Data Files e Enter the directory path of the desired data file The complete directory path must be entered for example c program files Beckman Coulter multimode reader simulatio
103. ique Type Selecting the Technique Type Use Technique Type to select the measurement technique used with the protocol Only techniques supported by the instrument are available For more information about technique types see Creating and Editing Detection Methods To select a technique type 1 In Technique Type choose the desired technique 2 Choose Next to make a Labware Selection see Selecting Labware OR Choose Back to configure protocol General Settings see Configuring General Settings OR Choose Cancel to clase Create Protocol without saving the new protocol Figure 1 2 Using online help nstalling Setting Up and Using the Software 1 5 ae 1 3 Installing Multimode Detection Software Installing Multimode Detection Software requires e Meeting System Requirements Section 1 3 1 Using the Setup Program to Install the Software Section 1 3 2 1 3 1 Meeting System Requirements To install and use Multimode Detection Software successfully the host computer must meet the minimum system requirements listed in Table 1 1 Where relevant Table 1 1 also lists recommended specifications Table 1 1 Host computer System Requirements Component Minimum Requirements CPU Pentium II 300 Mhz RAM 128 MB minimum 256 MB recommended Hard Drive 150 MB free space CD ROM Drive 4X Monitor 640x480 resolution Keyboard 101 key Mouse IBM compatible Serial
104. l 0000 appears in the Instrument Selection List Figure 1 10 When communications are configured correctly and the instrument is detected by the software the simulated instrument is replaced in the list by the connected instrument To configure communications settings 1 From the navigation pane choose Instruments The Instrument Selection List appears Figure 1 10 as Multimode Detection Software Simulated 5 01 x File Actions Help Instrument Selection List Name Serial Measurement techniques Multimode Reader 0000 Absorbance Luminescence Fluorescence Intensity Top Fluorescence Intensity Bottom Fluorescence F Simulated instrument Figure 1 10 Instrument Selection List with simulated instrument selected Multimode Detection Software User s Manual 1 14 Installing Setting Up and Using the Software y Figure 1 11 OR Ea 2 From the toolbar choose Instrument Settings Instrument Settings appears From the Actions menu choose Instrument Settings as Instrument Settings Basic Settings Filter Slides Manual Control Communication Settings Instrument Pott on E information fields Baudrate 38400 X Instrument Name Serial Number fooos Instrument Type fi 23 ed Fluorescence Firmware Version v0 1 03 16 2004 itive Temperature Controlled Features PIC Pw Version v1 0 16 03 04 Figure 1 11 Configuring communications settings
105. le to view Graphic View appears Figure 6 5 Refer to Section 6 2 3 1 Viewing Detailed Graphs of Individual Samples for more information about viewing and printing detailed graphs To exit Graphs Choose a different results tab to view OR Choose Finish to close Run Protocol Measurement results are saved and or prepared for printing when file and print options are defined in the protocol refer to Section 5 2 6 Configuring Output Settings Beckman Coulter Inc Viewing Measurement Results 6 7 6 2 3 1 Viewing Detailed Graphs of Individual Samples Graphic View displays a detailed graph of the measurement results for an individual sample Two types of graphs are available e Area scan measurements display a three dimensional graph that may be rotated horizontally and vertically refer to Section 6 2 3 1 1 Viewing and Printing an Area Scan Graph e Kinetic and linear scan measurements display a two dimensional graph that may be zoomed refer to Section 6 2 3 1 2 Viewing and Printing a Kinetic or Linear Scan Graph 6 2 3 1 1 Viewing and Printing an Area Scan Graph Area scan measurements display a three dimensional graph with the optical density OD of all points measured color coded Figure 6 5 The graph may be rotated horizontally and vertically and printed fl Graphic iew File WellA1 Use the vertical scroll bar to rotate the graph vertically ROm Use the horizontal scroll b
106. lecting the Labware Type Used in the Protocol OR Choose Cancel to close Create Protocol without saving the new protocol 5 2 5 Configuring Detection and Preparation Methods Use Method Selection to select and configure detection and preparation methods Figure 5 7 Two configuration modes are available e Basic a single detection method may be selected and optionally configured as a kinetic or scan measurement if desired Microplate shaking may also be configured refer to Section 5 2 5 1 Configuring a Method in Basic Mode e Advanced provides the configuration options available in basic mode and adds the ability to define multiple detection methods and to configure preparation methods such as shaking and temperature control 880 only Detection and preparation methods may be arranged in any order within the execution sequence refer to Section 5 2 5 2 Configuring a Method in Advanced Mode Ze Create Protocol Absorbance260 NE Method Selection General Settings Technique Type Select from available methods below Click Next to proceed Click Back to go back Labware Selection Layout Settings Method Selection gt P Advanced New Method Output Settings Method Selection Method Properties Jasse zeo hd Kinetic Shake Scan Toggle between basic and advanced modes Kinetic Determines if this method uses kinetic reads Figure 5 7 Configuring parameters in Me
107. lection List sayccscnnd exauundiensaasnaurstvensenteaasunttaansenesbasveaalenvedcuanianvanensuntanesens 5 2 Figure 5 2 Defining a protocol name and entering notes about the protocol ce ceeeeeeeeeee 5 3 Figure 5 3 Selecting the measurement technique for a DTX 880 o cee eeceseeseeeeeeeeeeeeeaeeneeees 5 4 Figure 5 4 Selecting the type of labware used in the protocol 0 cee eeceeeeeceeteeeeeeeeeeeeceeeeneeees 5 5 Figure 5 5 Selecting which wells are measured on a 384 well plate eee eseeseeeeeeteceeeeneeees 5 6 Figure 5 6 Selecting how wells on the plate are read 0 eee eeeeceseceeceeceeecneeeseeeeeeeeeaecaeeneeees 5 7 Figure 5 7 Configuring parameters in Method Selection cececeeecceseceteceeeeeeeeeeeeceseeneeeneeees 5 8 Figure 5 8 Configuring a method in basic mode ss sssssssssensessesesssssesessesessestesessesesessersessesee 5 9 Figure 5 9 Configuring an area Scan sesssesssessessersrssresresstsstssressesstesttsstestsseesteseestesseeseesee 5 11 Fig r 5 10 Configuring a linear SCAM c pescencsssneiedesusenescsassinamseveneduerotsussusuastestaunseeswuecsusinscions 5 12 Figure 5 11 Configuring a method in advanced mode 0 eeccesesseeseeeeeeeeeeeeeeceaecnseeeeeeeeeees 5 13 Figure 5 12 Configuring a Shake preparation method c sccsssssrscosssrcssssrecssorsnnssserssonsees 5 15 Figure 5 13 Configuring a Set Temperature preparation method esesssessssssssssesressssesses
108. lection List select the desired instrument Figure 2 1 2 From the toolbar choose Load the plate carrier OR From the Actions menu choose Load the plate carrier OR Right click on the desired instrument and choose Load the plate carrier from the menu that appears Multimode Detection Software User s Manual 2 4 Setting Up and Controlling Instruments 2 2 3 Initializing the Instrument Initializing the instrument moves the optics and microplate transports to home positions Each time the instrument is turned on an initialization procedure is automatically performed If necessary the instrument may be initialized manually for example after an emergency stop has been performed refer to the DTX 800 880 Multimode Detectors User s Manual Section 1 3 1 1 Performing an Emergency Stop Note When a hardware error occurs turning the instrument off and on is recommended The instrument initializes automatically on power up and will not require manually initializing the instrument using the software To manually initialize the instrument 1 In the Instrument Selection List select the desired instrument Figure 2 1 2 From the toolbar choose Initialize the instrument DJ o From the Actions menu choose Initialize the instrument OR Right click on the desired instrument and choose Initialize the instrument from the menu that appears 2 2 4 Connecting to the Instrument When Multimode Detection Software is launched
109. leting detection methods 3 16 labware 4 11 protocols 5 30 detection methods configuring absorbance 3 6 configuring fluorescence intensity bottom 3 9 configuring fluorescence intensity top 3 8 configuring fluorescence polarization 3 10 configuring in protocols 5 8 configuring luminescence 3 7 configuring time resolved fluorescence 3 11 copying 3 15 creating 3 3 deleting 3 16 editing 3 13 selecting absorbance type 3 4 selecting for labware optimization 4 13 selecting technique 3 3 E editing detection methods 3 13 labware 4 7 labware dimensions 4 8 labware lots 4 9 protocols 5 28 well information 4 8 error codes A 1 F filter slides adding 2 8 configuring 2 9 exporting and importing 2 10 overview 2 8 removing 2 10 Multimode Detection Software User s Manual fluorescence intensity bottom detection methods configuring 3 9 fluorescence intensity top detection methods configuring 3 8 fluorescence polarization detection methods configuring 3 10 viewing results 6 12 H host computer removing software from 1 9 system requirements 1 5 l instrument connecting to 2 4 initializing 2 4 instrument selection list overview 2 1 instrument settings configuring 2 6 filter slides 2 8 manual control 2 11 emission filter slide control 2 12 excitation filter slide control 2 12 plate control 2 12 temperature control 2 11 K kinetic measurements configuring 5 9 viewing graph 6 8 L labware configuring layou
110. ment e Set Value manually sets the reading height using the adjacent configuration field Setting the reading height manually is useful when the height determined by a prior optimization for the same labware type is known OR Choose Run or Next to run the protocol Choose Cancel to close Run Protocol without running the protocol Defining Editing and Running Protocols 5 23 5 3 1 1 Optimizing Read Height 880 only The DTX 880 features an objective lens that may be moved up and down to optimize the read height used in fluorescence intensity top fluorescence polarization and time resolved fluorescence protocols Read height is the distance between the top surface of the microplate being read and the lower surface of the objective lens Optimizing read height matches the focus of the optics with the sample volume This maximizes the raw signal which yields the highest precision and maximum sensitivity Read height is optimized using the Read Height Optimization Wizard Figure 5 17 A single sample with a known maximum signal is placed on the same type of microplate used in the protocol The sample is measured using the same or very similar detection method used in the protocol The optimized read height is saved in the protocol and is used for all subsequent runs of the protocol until reset by performing a new optimization or manually selecting a read height option To optimize read height 1 In Run Proto
111. meters Method Name Fluorescent Excitation Filter nm essi CYS Emission Filter nm sss H Integration Time seconds fo SSS Method Parameters gt Date created 1 28 2004 Figure 3 10 Editing a fluorescence intensity top method Multimode Detection Software User s Manual 3 14 Creating and Editing Detection Methods 3 Edit the method parameters as desired For more information about the parameters for a specific detection method refer to the section that covers defining the desired detection method Defining Absorbance Method Parameters Section 3 2 3 1 Defining Luminescence Method Parameters Section 3 2 3 2 Defining Fluorescence Intensity Top Method Parameters Section 3 2 3 3 Defining Fluorescence Intensity Bottom Method Parameters 880 only Section 3 2 3 4 Defining Fluorescence Polarization Method Parameters 880 only Section 3 2 3 5 Defining Time Resolved Fluorescence Method Parameters 880 only Section 3 2 3 6 4 Choose Save to close the Method Editor and save the changes OR Choose Cancel to close the Method Editor without saving changes Beckman Coulter Inc 3 4 Creating and Editing Detection Methods 3 15 Copying Detection Methods Copies may be made of existing detection methods After a copy has been created it may be used as a template for a new detection method using the same method technique To make a copy
112. n each time a protocol or measurement results are printed In Print Options choose Show printer settings to display printing options each time a protocol or measurement results are printed Choose Apply to save the new print settings Multimode Detection Software User s Manual 1 18 Installing Setting Up and Using the Software 1 4 2 3 Choosing Simulated Data Files Protocols may be run in simulation mode which allows the protocol configuration to be tested using simulated data before performing the protocol on actual samples In simulation mode all features for the instrument type currently selected in the Instrument Selection List are available but measurement results are either randomly generated by the software or read from a data file Use Simulated Data to choose the default data files for simulated absorbance luminescence and fluorescence measurements Figure 1 15 a Multimode Detection Software Connected Filter Slides Ex 2 Em 1 Software Settings Language Print Settings Simulated Data Directory Settings Absorbance Simulated Data File c program files beckman coulter inc multimode detection software templates ABS965i Luminescence Simulated Data File c program files beckman coulter inc multimode detection software templates Lumi965 Fluorescence Simulated Data File c program files beckman coulter inc multimode detection software templates Fluora9t coke Browse for desired data
113. n MEASUFEMENA ee ceeeeeteeneeesecneeeseeeeeeeeeaeenaeeneeens 6 3 Figure 6 3 Raw Data displayed for a kinetic absorbance measureMent ceeeeeeeeeeeeeeeteeees 6 4 Figure 6 4 Viewing area scan measurement graphs ceeeeeeeeceseceseceeecseeeseeeeeesecaecnaecaeeeneees 6 6 Figure 6 5 Three dimensional area scan GAG Nssecsussssscdeenivesnesissesssaverdsradennscauevensdvensinteaduwcasnes 6 7 Figure 6 6 Two dimensional kinetic graph with zoom region selected eceeceeseeseeeeees 6 8 Beckman Coulter Inc Figure 6 7 Viewing protocol information in the General worksheet excerpt 00 00 seers 6 10 Figure 6 8 Viewing absorbance data in a Cycle worksheet excerpt ccceeeeceeseeeteeeeeeeees 6 11 Figure 6 9 Viewing fluorescence polarization data CXCerpt cccceccseceseceeeceeeeeeeeceseeeeeeees 6 12 Figure 6 10 Previewing a measurement results printout 00 0 0 ceecceceeseeeteceteceeeeeeseeesseenteenees 6 13 Multimode Detection Software User s Manual Installing Setting Up and Using the Software Overview Multimode Detection Software configures and controls all measurement protocols and operations performed by the DTX 800 and DTX 880 Multimode Detectors Absorbance glow luminescence fluorescence intensity top and bottom fluorescence polarization and time resolved fluorescence TRF measurements are all supported The measurement methods available to users depend on the capabilitie
114. nce wavelength This measurement is subtracted from the first to calculate the final result 5 Method Editor Method Parameters Method Technique Method Type Method Name Jabs620 Method Parameters gt Excitation Filter nm fezo SL2 Reference Excitation Filter nm Date created 3 1 2004 Reference Excitation Filter Appears only when configuring bichromatic measurements Cancel Figure 3 4 Defining absorbance measurement parameters To define absorbance method parameters 1 Enter a Method Name Figure 3 4 2 In Excitation Filter nm select the measurement filter 3 Ifa bichromatic measurement is being defined in Reference Excitation Filter nm select the reference filter Note The filters available are those installed on the excitation filter slide loaded in the instrument and configured for absorbance techniques in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 4 Choose Save to save the new absorbance detection method The new method appears in the Detection Method Selection List OR Choose Back to define a different Method Type refer to Section 3 2 2 Selecting the Type of Absorbance Method OR Choose Cancel to close the Method Editor without saving the new detection method Beckman Coulter Inc Creating and Editing Detection Methods 3 7 3 2 3 2 Defining Luminescence Method Parameters A luminescence method performs glow lumine
115. ng on how Print Settings are configured Print and or Print Preview may appear before the measurement results print Refer to Section 1 4 2 2 Configuring Print Settings for information about enabling and disabling Print and Print Preview To print measurement results 1 When the measurement is complete press Finish If Print and Print Preview are not enabled in Print Settings no additional configuration is required The measurement results print out 2 If Print appears configure printing options as desired and choose OK OR If Print Preview appears use the toolbar controls to change the magnification layout view or page s displayed if desired Figure 6 10 Jl Print preview oxi si CA eee L ll Change page s nes hak layout view Date Friday April 23 2004 Time 11 53 21 AM Change view pplication Type magnification Date Measured Date Evaluated Valid Labeware Name Used Wells Lot Lot Optimized Protocol Definition Absorbance 260 Shaking DefaultLot Wednesday March 24 2004 Absorbance 260 Shaking BasicApplication Friday April 23 2004 i Thursday April 15 2004 Defined 7 Ab 384 Well Reaction Plate 24 x 16 384 Measurement Method 2 Measurement Technic 1 1 1 3 748 1 11 1 1 2 1 2 647 1 12 1 ABS 260 Absorbance 1 4 1 0 336 1 14 1 1 5 1 2 345 1 15 1 1 6 1 2 997 1 16 1 1 7 1 1 8 1 0 404 2 132 1 17 1_ 1 18
116. ngs The Basic Settings tab Figure 2 2 contains information about the connection from the instrument to the operating computer as well as identifying information about the instrument The Port and Baudrate must be configured correctly to enable Multimode Detection Software to communicate with the instrument aS Instrument Settings Basic Settings Filter Slides Manual Control Communication Settings Instrument Port on a information fields Baudrate 38400 m Features Instrument Name Serial Number fooos Instrument Type C Firmware Version V0 1 03 16 2004 PIC Pw Version v1 0 16 03 04 Figure 2 2 Instrument Settings Basic Settings To configure basic instrument settings Setting Up and Controlling Instruments 2 7 1 In Instrument Settings select Basic Settings to display the Basic Settings tab if necessary 2 In Port select the serial communications port on the host computer that connects to the instrument 3 In Baudrate select 38400 4 Choose Apply to detect the instrument When the instrument is detected the instrument information fields are autopopulated with information about the connected instrument Refer to Table 2 1 for more information about each field Table 2 1 Basic Settings Read Only Fields Field Instrument Name Description The model of the instrument Serial Number The serial number of the instrument Instrument Type
117. ngs Technique Type Select output options for Excel export and printer settings Labware Selection Layout Settings Method Selection Be Export to Microsoft Excel LY Perform after completing measurement s 8 Create XML and dat data files LY Perform after completing measurement s Print options M Perform after completing measurement s V Protocol Information M Labware Information C General Information Execute program Run this protocol now V Execute a program after protocol executes Execute program Command line parameter Figure 5 15 Configuring an external software application in Output Settings 2 In Execute Program enter the complete path of the program to run for example C Program Files Data Logger MyCustomDataL ogger exe OR el Choose Browse to find an executable program Open appears 3 In Open browse to the location of the desired application and select it 4 Choose Open to return to Output Settings The selected path appears in Execute Program OR Choose Cancel to close Open without selecting an application 5 Ifthe selected application supports command line parameters in Command line parameter enter the desired parameter Beckman Coulter Inc 5 3 Defining Editing and Running Protocols 5 21 Running Protocols Saved protocols may be accessed and run at any time either on an instrument or in simulation mod
118. ns simulated absorbance data dat e Browse to the location where the desired data file is saved and select it Note Results from prior measurements saved in dat format may be used as simulated data files refer to Section 5 2 6 Configuring Output Settings Simulated data files are used when the number of measurement points in the simulated protocol run is the same as those present in the data file When the number of measurement points is different the software generates random data When a different simulated data file is selected in Prepare to Run Protocol the file is used for the current simulated run only After the simulated run has finished the data file defaults to the file selected in Software Settings refer to Section 1 4 2 3 Choosing Simulated Data Files Choose Run or Next to run the selected protocol OR Choose Cancel to close Run Protocol without running the protocol Multimode Detection Software User s Manual 5 28 Defining Editing and Running Protocols 5 4 Editing Protocols The parameters configured for user defined protocols may be edited Default protocols included with the software installation may not be edited To edit a protocol 1 In the Protocol Selection List select the protocol to edit 2 From the toolbar choose Edit the selected protocol OR From the Actions menu choose Edit the selected protocol OR Right click on the selected protocol and choose Edit t
119. nt is performed after a specified delay Several of these excitation measurement cycles may be performed on each sample When multiple excitation measurement cycles are performed the results from all cycles are used to calculate a single measurement result for each sample 5 Method Editor Method Parameters Method Technique Method Parameters gt Method Name Excitation Filter nm Emission Filter nm LED on Time seconds Number of Pulses Delay Before Measure seconds Integration Time seconds Date created Figure 3 9 Defining time resolved fluorescence method parameters Multimode Detection Software User s Manual foot lt s hoo i CS ooo ooo tt s S 5 2 2004 3 12 Creating and Editing Detection Methods Beckman Coulter Inc To define time resolved fluorescence parameters l 2 3 Enter a Method Name Figure 3 9 Select the Excitation Filter nm Select the Emission Filter nm Note The filters available are those installed on the excitation and emission filter slides loaded in the instrument and configured for time resolved fluorescence techniques in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides In LED on Time seconds enter the length of time in seconds that the LED light source remains turned on In Number of Pulses enter the number of excitation measurement cycles performed in the measurement
120. ods Beckman Coulter Inc Defining Editing and Running Protocols 5 19 Table 5 1 Output Options Output Option Description Export to Microsoft Excel Perform after completing Saves results in a format compatible with Microsoft measurement s Excel and automatically opens Excel when the protocol run is completed Note Versions of Excel prior to Office 2000 are not supported by the Export to Microsoft Excel function but can open measurement results stored in tab delimited data dat files Create XML and dat data files Perform after completing Automatically exports measurement results to a tab measurement s delimited data dat file and an XML file These files may be opened by software applications compatible with tab delimited data or XML files Note The directory where the data files are saved is configured in System Settings Refer to Section 1 4 2 4 Choosing the Directory Where Measurement Results are Saved Print options Perform after completed Automatically prints the results after completing a measurement s protocol run This option must be selected to print measurement results Raw Data Prints raw data measured for each sample For kinetic measurements prints raw data measured in each cycle For scan measurements prints raw data for all measurement points Note Printing kinetic and scan measurement results may require numerous pages depending on the number o
121. odules Selection and configuration pane Navigation pane Figure 1 1 Multimode Detection Software main window Beckman Coulter Inc Installing Setting Up and Using the Software 1 3 1 2 2 Using the Software Interface Multimode Detection Software uses a simple interface that is divided into three basic sections e Navigation pane refer to Section 1 2 2 1 About the Navigation Pane e Toolbar refer to Section 1 2 2 2 About the Toolbar e Selection and configuration pane refer to Section 1 2 2 3 About the Selection and Configuration Pane The navigation pane provides access to the five modules that provide the majority of the functionality built into the software The options available in the toolbar and selection and configuration pane are determined by the module currently selected in the navigation pane 1 2 2 1 About the Navigation Pane The navigation pane is the narrow pane on the left of the Multimode Detection Software window Figure 1 1 Use the navigation pane to switch between modules e Protocols opens the Protocol Selection List and provides the ability to define run edit copy delete and print measurement protocols refer to Chapter 5 Defining Editing and Running Protocols Detection Methods opens the Detection Method Selection List and provides the ability to create edit copy and delete detection methods refer to Chapter 3
122. of a detection method 1 In the Detection Method Selection List select the detection method to copy 2 From the toolbar choose Make a copy of the selected method OR From the Actions menu choose Make a copy of the selected method OR Right click on the selected detection method and choose Make a copy of the selected method from the menu that appears Note The default name format for copied detection methods is Copy of OriginalName To change the name edit the detection method refer to Section 3 3 Editing Detection Methods Multimode Detection Software User s Manual 3 16 Creating and Editing Detection Methods 3 5 Deleting Detection Methods User created detection methods not used in any measurement protocols may be deleted from the Detection Method Selection List Default detection methods provided with the software and user created methods used in protocols may not be deleted To delete a detection method 1 In the Detection Method Selection List select the detection method to delete 2 From the toolbar choose Delete the selected method OR From the Actions menu choose Delete the selected method OR Right click on the selected detection method and choose Delete the selected method from the menu Message appears Figure 3 11 e Figure 3 11 Confirming the deletion of a detection method 3 Choose Yes to delete the selected detection method OR Choose No to cancel the deletion
123. oftware User s Manual
124. on remains depressed while the instrument is in simulation mode OR From the Actions menu choose Simulate the current instrument OR Right click on the desired instrument and choose Simulate the current instrument from the menu that appears Multimode Detection Software User s Manual 2 6 2 3 Beckman Coulter Inc Setting Up and Controlling Instruments Configuring Instrument Settings Communication settings and filter slides for the instrument are configured in Instrument Settings Configuring the instrument in Instrument Settings informs Multimode Detection Software about the instrument such as the communication speed and port used and the configuration of filter slides and individual filters Instrument features such as microplate shaking may also be controlled manually To configure instrument settings 1 Select the desired instrument in Instrument Selection List 2 From the toolbar choose Instrument Settings Instrument Settings appears OR From the Actions menu choose Instrument Settings OR Right click on the desired instrument and choose Instrument Settings from the menu that appears 3 Configure instrument settings on the three tabs as described in the following sections e Configuring Basic Instrument Settings Section 2 3 1 e Defining and Editing Filter Slides Section 2 3 2 e Manually Controlling the Instrument Section 2 3 3 2 3 1 Configuring Basic Instrument Setti
125. on 4 2 Editing Labware Section 4 3 Copying Labware Section 4 4 Deleting Labware Section 4 5 Optimizing Labware Section 4 6 Exporting and Importing Labware Section 4 7 Multimode Detection Software User s Manual 4 2 Creating and Editing Labware To define and edit labware From the navigation pane choose Labware The Labware Selection List appears Figure 4 1 H Multimode Detection Software Connected Filter Slides Ex 2 Em 1 File Actions Help Labware functions Labware Selection List Name Number of Wells Created Last Edited Last Optimization Ab 384 Well Reaction Plate 384 21812004 3 24 2004 3 24 2004 96 12 3 2003 4 5 2004 4 5 2004 96 12 3 2003 12 3 2003 12 3 2003 384 21812004 21812004 218 2004 12 2 15 2004 2 23 2004 2 23 2004 24 2115 2004 2 23 2004 2 23 2004 48 2 15 2004 2 23 2004 2 23 2004 6 2115 2004 2 23 2004 2 23 2004 6 2 15 2004 4 5 2004 4 5 2004 Costar Cone 96 Round 96 21812004 21812004 218 2004 Labware costar Flat 384 Square 384 21812004 21812004 2 8 2004 Greiner 24 24 2 15 2004 2 23 2004 4 5 2004 Greiner 384 Cone PP 384 21812004 2 8 2004 2 8 2004 Greiner 384 Flat pp 384 2 8 2004 2 8 2004 2 8 2004 Greiner 384 Thermal Cycler 384 2 8 2004 2 8 2004 2 8 2004 Greiner 6 6 2 15 2004 2 23 2004 2 23 2004 Greiner 96 Cone PP 96 2 8 2004 2 8 2004 2 8 2004 Greiner 96 Cone PS 96 2 8 2004 2 8 2004 218 2004 Greiner 96 Flat 96 2 8 2004 2 8 2004 218 200
126. on pane choose Detection Methods The Detection Method Selection List appears Figure 3 1 8 Multimode Detection Software Connected Filter Slides Ex 2 Em 1 File Actions Help Detection methods All detection method functions are accessed from the Detection Method Selection Detection method functions Detection Method Selection List Name Measurement technique Measurement type Created Last Edited AB5 260 Fluoroscein Bottom Rhodamin Bottom ABS 595 ABS 450 ABS 340 Fluorescein Top ABS 405 ABS 620 Luminescence Rhodamin Top TRF Europium TRF Europium Absorbance FluorescenceIntensityBottom FluorescenceIntensityBottom Absorbance Absorbance Absorbance FluorescencelntensityTop Absorbance Absorbance Luminescence FluorescenceIntensityTop TimeResolvedFluorescence TimeResolvedFluorescence Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 Monochromatic 1 28 2004 List Available detection method functions are Beckman Coulter Inc Creating Detection Methods Section 3 2 Editing Detection Methods Section 3 3 Copying Detection Methods Section 3 4 Deleting Detection Methods Section 3 5 1
127. on the microplate used in the optimization The liquid volume of the optimization sample should be the same as that of samples measured in the protocol 5 Load the plate into the instrument Read Height Optimization Wizard Hex Prepare Labware Select a Detection Method Prepare the labware by pipetting liquid into the well that will be optimized Please select that well below Make Prepare the Labware gt sure you enter a minimum value that you expect from your sample Click Next to proceed Optimize 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 l Select the well containing the optimization sample Optimization Complete Figure 5 18 Selecting the well read in the read height optimization 6 In Prepare Labware select the well containing the optimization sample Figure 5 18 7 Choose Next to start the optimization Optimization in Progress appears Figure 5 19 The optimization may take several minutes OR Choose Back to change the detection method used in the calibration OR Choose Cancel to close the wizard without performing the optimization Beckman Coulter Inc Defining Editing and Running Protocols 5 25 d Read Height Optimization Wizard Optimization in Progress Reading height is currently being optimized Please wait Optimization Complete Optimizing Estimated Time 00 05 43 Figure 5 19 Read height optimization in
128. parate worksheet The mP calculated for each sample is considered approximate because Parallel polarization plane raw data Perpendicular polarization plane raw data Calculated mP Beckman Coulter Inc Blank samples are not defined at run time but are required to increase the accuracy of the results The G factor used in the calculation which factors out differences in detection efficiency between the polarization planes is derived from fluorescein measurements performed on a number of DTX 880 instruments Note entered in the G factor cell in the spreadsheet Minor deviations between the actual G Factor present in the measurement and the average G factor used in the calculation result in a common shift for all mP values measured Differences in mP between samples and controls are not affected to a first order approximation If a more accurate G factor has been determined for the instrument it may be El Microsoft Excel Fluorescence Polarization _FP 485 0_04 23 2004_01 46 02 60 xml Data Window File Edit view Insert Format Dehean ay s A27 Cycle Temperature Time MatrizFormat Parallel 1 2 3 4 5 6 7 8 X amp 5 Tools pa Jlor Help 2 Kt Mb 75 2 _ Atrial Unicode MS 1300334 2008887 3735267 2563314 1543516 3608513 2227146 2509960 1 779350 169006 2790455 895296 2632831 761667 1991226 501359
129. pe Optimized with Absorbance Property Serial grid B Labware Lot Measurements Lower left x offset 1 435 Lower left y offset 1 135 Lower right x offset 1 435 aes 1 135 Upper left x offset 1 435 Upper left y offset 1 135 Upper right x offset 1 435 Upper right y offset 1 135 E Well Measurements Column distance 0 9 Row distance 0 9 x Lower right y offset 7 Offset from lower right corner of labware to Information about the first row cm field being configured Figure 4 3 Defining offsets and well dimensions 3 If necessary click the next to Well Measurements to display the fields in the category In Column Distance enter the distance between columns In Row Distance enter the distance between rows Enter the Well depth pl Oy ae i In Well Length enter the length of the well in the direction of the columns on the plate 8 In Well Width enter the length of the well in the direction of the rows on the plate 9 In Notes enter information about the labware lot or configuration if desired 10 Choose Save to save the new labware and close the Labware Editor OR Choose Back to edit Plate Information refer to Section 4 2 1 Defining Labware Information OR Choose Cancel to close the Labware Editor without creating new labware Beckman Coulter Inc 4 3 Creating and Editing Labware 4 7 Editing Labware Dimensions and information for user defined labwa
130. pecified in this manual regardless of foreseeability or the form of action whether in contract tort including negligence breach of warranty strict liability or otherwise and including but not limited to damages resulting from loss of data loss of anticipated profits or any special indirect incidental or consequential damages In no event shall Beckman Coulter Inc s liability to the user exceed the amount paid by the user to Beckman Coulter Inc hereunder The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results Beckman Coulter SAGIAN Operations Indianapolis Indiana www beckmancoulter com Biomek SAMI Orca and the Beckman Coulter logo are registered trademarks and Beckman Coulter and SAGIAN are trademarks of Beckman Coulter Inc Microsoft Windows and Windows NT are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries IBM is a trademark of International Business Machines Corporation in the United States other countries or both Pentium is a registered trademark of Intel Corporation Adobe Acrobat and Postscript are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries All other trademarks appearing in this manual are the property of their respective owners Copyright 2004 by Beckman Coulter Inc All rights r
131. progress 8 While the optimization is in progress choose Cancel to stop the optimization read and close the wizard if desired When the read is finished Optimization Complete appears displaying the Optimized Read Height Figure 5 20 dp Read Height Optimization Wizard Optimization Complete Note optimized read height below Optimization in complete click finish to save results Optimization Complete gt Optimized Read Height 0 97 mm Figure 5 20 Read height optimization completed 9 In Optimization Complete choose Save to save the optimized read height in the protocol The optimized read height is used for all subsequent runs of the protocol until reset by performing a new optimization or manually selecting a read height option OR Choose Cancel to close the wizard without saving and using the optimized read height in the protocol run Multimode Detection Software User s Manual 5 26 Defining Editing and Running Protocols 5 3 2 Running a Protocol When Simulation Mode is Enabled Running a protocol in simulation mode allows the protocol configuration to be tested using simulated data before performing the protocol on actual samples Simulated data is either generated randomly or read from a file Simulation mode is automatically enabled when the host computer is not connected to an instrument When an instrument is connected simulation mode may be enabled manually in Instruments refer to Sect
132. re C Remove Multimode Detection Software Cancel Bach Figure 1 7 Setup wizard repair or remove the software installation 8 Select Repair Multimode Detection Software to search for and automatically reinstall any missing or damaged files required to run the software OR Select Remove Multimode Detection Software to remove the application and support files from the host computer Note When removing the software only files installed by the setup program during the initial installation are removed Files created after the installation such as exported measurement results and labware detection method and protocol definitions are not removed Measurement results files may still be viewed after the software is uninstalled All file types may be copied archived or manually deleted Choose Finish to perform the selected action OR Choose Cancel to close the setup wizard without repairing or removing the software Installing Setting Up and Using the Software 1 11 1 4 Setting Up Multimode Detection Software After installing the software and physically connecting the instrument to a serial port on the host computer communications and software settings must be set up Setting up the software includes e Configuring Communication Settings Section 1 4 1 e Configuring Software Settings Section 1 4 2 To set up Multimode Detection Software 1 From the Windows Start menu choose Progr
133. re not supported by the Export to Microsoft Excel function but can open measurement results stored in tab delimited data dat files e Create XML and dat data files saves results in xml and tab delimited data dat files which may be opened by compatible software applications Note Refer to Section 5 2 6 Configuring Output Settings for more information about configuring export and file options To view saved measurement results 1 Open the desired software application 2 Browse to the directory where exported measurement results are stored and open the desired file Saved measurement results are stored in the data directory selected in Software Settings refer to Section 1 4 2 4 Choosing the Directory Where Measurement Results are Saved 6 3 1 Viewing Measurement Results Using Microsoft Excel When Export to Microsoft Excel is defined in the protocol protocol information and measurement results are exported to a file and automatically opened in Excel after completing a protocol run Each exported Excel spreadsheet contains data for a single protocol run Data is presented in a series of worksheets The first worksheet General contains information about the protocol refer to Section 6 3 1 1 Viewing Information About the Protocol Raw data is contained in separate worksheets for each measurement cycle refer to Section 6 3 1 2 Viewing Measurement Results Multimode Detection Software User s Manual
134. re not used in measurement protocols may be edited For default labware included in the software installation or labware used in protocols dimensions and information may be viewed but not edited When labware lots have been created by optimizing the labware to compensate for dimensional variations between production lots the active lot may be changed and or edited The active lot may be selected and or edited for all labware in the Labware Selection List Note Labware lots are created by optimizing the labware refer to Section 4 6 Optimizing Labware Labware is edited in the Labware Editor Figure 4 4 and includes e Viewing and Editing Labware Information Section 4 3 1 e Selecting and Editing Labware Lots Section 4 3 2 To view and edit labware dimensions and information 1 In the Labware Selection List select the labware to edit 2 From the toolbar choose Edit the selected labware OR From the Actions menu choose Edit the selected labware OR Right click on the selected labware and choose Edit the selected labware from the menu that appears Multimode Detection Software User s Manual 4 8 Creating and Editing Labware Beckman Coulter Inc 4 3 1 Viewing and Editing Labware Information Use Labware Information to view and edit labware dimensions information and supported techniques Plate information for default labware included in the software installation and labware used in pro
135. ring a Shake Preparation Method for more information about shaking Refer to Section 5 2 5 2 2 Configuring a Set Temperature Preparation Method 880 only for more information about configuring temperature control in the protocol Add and configure additional preparation methods by repeating steps 5 and 6 for each method desired To reorder the detection and preparation methods in the method execution sequence select the method to be moved and choose Move the selected method forward or Move the selected method backward OR In Method Selection right click on the desired detection or preparation method and choose Move Up or Move Down To delete a detection or preparation method from the protocol in Method Selection select the method to be deleted and choose Delete the selected method from this protocol OR In Method Selection right click on the desired detection or preparation method and choose Remove Method Defining Editing and Running Protocols 5 15 10 Choose Next to configure Output Settings refer to Section 5 2 6 Configuring Output Settings OR Choose Back to change Labware Layout Settings refer to Section 5 2 4 Configuring Labware Layout Settings OR Choose Cancel to close Create Protocol without saving the new protocol 5 2 5 2 1 Configuring a Shake Preparation Method A Shake preparation method shakes the microplate at the point in the execution sequence where it is positione
136. rs Contact a Beckman Coulter Service Engineer Z transport adjust error Possible causes wrong well for adjustment low fluorescein concentration In the Read Height Optimization Wizard confirm that the correct well is selected for the optimization read refer to Section 5 3 1 1 Optimizing Read Height 880 only 10 Measurement attempted with no labware loaded Load a plate into the instrument Multimode Detection Software User s Manual A 2 Software Error Codes Table A 1 Software Error Codes and Solutions Error Code 11 Error Message The current labware definition is invalid Recommended Solution Check plate parameters configured in Labware Information refer to Section 4 2 1 Defining Labware Information 12 Detection method parameters are invalid Check the configuration of detection method parameters 13 The current labware format is not supported Make sure the plate type has been defined refer to Section 4 2 Creating Labware Currently loaded labware is not in the correct orientation Load the plate into the instrument with the orientation configured in the protocol Linear scan range exceeds well diameter Reduce the number of measurement points in the scan configuration refer to Section 5 2 5 1 Configuring a Method in Basic Mode Bright measurement below limit Check Platedefinition Check plate parameters
137. s of the instrument being controlled Measurement results are easily exported and may be evaluated with an external application such as Microsoft Excel The simple interface divides the main window into three basic sections which provide easy access to system functionality Most operations use a wizard type interface to efficiently guide the task being performed This user s manual provides comprehensive coverage of Multimode Detection Software functionality including Setting Up and Controlling Instruments Chapter 2 Creating and Editing Detection Methods Chapter 3 e Creating and Editing Labware Chapter 4 Defining Editing and Running Protocols Chapter 5 e Viewing Measurement Results Chapter 6 This chapter introduces users to the software and provides instructions for e Using Multimode Detection Software Section 1 2 Installing Multimode Detection Software Section 1 3 Setting Up Multimode Detection Software Section 1 4 Multimode Detection Software User s Manual 1 2 Installing Setting Up and Using the Software 1 2 Using Multimode Detection Software Multimode Detection Software uses a simple interface that divides the main window into three basic sections navigation pane toolbar and selection and configuration pane Figure 1 1 The interface includes easy access to the five modules that provide system functionality and a comprehensive conte
138. s 3 16 Figure 3 12 Choosing the folder where an exported detection method will be saved 3 17 Figure 4 1 Accessing labware TIMCTIOMNS ssicezescencetavsreraiierandcnsvsnncendn ordeal daca eanas 4 2 Figure 4 2 Defining plate dimensions and information ccceecceseceeceeeceseeneeeseeeeeeeeeeaecnaeeneeees 4 3 Figure 4 3 Defining offsets and Well dimensions ejacvsusivenicssdicavaceednoveraautinetaasiundunoisuonnaacedys 4 6 Figure 4 4 Editing labware dimensions and well information ssssssssssssssssseseesessrserssssersessesee 4 8 Figure 4 5 Configuring offsets and well dimensions in Labware Lots csceeseeceeeeeeteeneeees 4 9 Figure 4 6 Confirming a labware deletion eeccceccceceesceeseeceseceeceeeeeeseecseceseeeseecsaeeneesaees 4 11 Figure 4 7 Selecting the detection method for labware optimization ceseeeeseneereeeeees 4 13 Figure 4 8 Preparing the labware for Optimization cccceccccceseceeceeeeceeeceeeeeeeeeseecsaeeneenees 4 14 Figure 4 9 Labware optimization in Progress ccccccccesceseeesseceeeceeeceeeeeeseecsaeceseeeeeeeeseeesaeenaes 4 15 Figur 4 10 Selecting th well Center sect caieesccasienevnneancne anes 4 16 Figure 4 11 Verifying well centers nsenesseseesseessessossecssesssesorsseerensorsrsseesonssrsseersesorsseesseseso 4 17 Figure 4 12 Choosing the folder where exported labware will be saved ss nssssssessssesssssses 4 19 Fig re 5 1 Protocol Se
139. s at all times Because of this importing default labware from an XML export file is not permitted by the software To export labware 1 In the Labware Selection List select the labware to export 2 From the File menu choose Export gt Labware Browse for Folder appears Figure 4 12 My Documents 4 This Computer amp My Network Places g Recycle Bin Exported Protocols Make New Folder Figure 4 12 Choosing the folder where exported labware will be saved 3 In Browse for Folder browse to the folder where the exported labware will be saved OR Choose Make New Folder to create a new folder where the exported labware will be saved 4 Choose OK to export the labware The exported labware is saved using the default file name format Labware_LabwareName xml In order to import the file at a later date the filename must not be changed OR Choose Cancel to stop the operation without exporting labware Multimode Detection Software User s Manual 4 20 Creating and Editing Labware To import labware from an exported XML file 1 From the File menu choose Import gt Labware Open appears 2 In Open browse to and select the desired XML file to import 3 Choose Open The labware is imported to the Labware Selection List Beckman Coulter Inc Defining Editing and Running Protocols 5 1 Overview A protocol stores all parameters required to perform a measurement inclu
140. scence measurements on samples Generally luminescence measurements do not require a filter however cutoff filtration using an emission filter may be specified when eliminating photoluminescence generated by the microplate itself is desired us Method Editor Method Parameters Method Technique Method Parameters gt Method Name Emission Filter nm Integration Time seconds Date created 5 2 2004 Cancel Figure 3 5 Defining luminescence measurement parameters To define luminescence method parameters 1 Enter a Method Name Figure 3 5 2 In Emission Filter nm select the cutoff filter Note Most luminescence measurements do not require cutoff filtration The filters available are those installed on the emission filter slide loaded in the instrument and configured for luminescence techniques in Instrument Settings refer to Section 2 3 2 Defining and Editing Filter Slides 3 In Integration Time seconds enter the length of time in seconds the signal is collected from samples The integration time may be set within the range of 0 00005 to 3600 seconds 4 Choose Save to save the new luminescence detection method The new method appears in Detection Method Selection List OR Choose Back to select a different Method Technique refer to Section 3 2 1 Selecting a Method Technique OR Choose Cancel to close the Method Editor without saving the new detection method Multimode Detection Softw
141. ser s Manual viii 2 Setting Up and Controlling Instruments 2 1 OVENI E Wa arent orate a E E E A E EAE EE eee ee ee 2 1 2 2 Controlling Instrument Functions nessas seuas aeaaeae 2 3 2 2 1 Wjectne the Plate Cater 145424 54 386eide8 ae eed ease rekene 2 3 2 2 2 Loading the Plate Carrier 5 ccccsceviacviaecvansstsesvnesas 2 3 2 2 3 Initializing the Instrument 0 0 0 00 0 2 4 2 2 4 Connecting to the Instrument 0 0 00 2 4 2 2 5 enabling Simulation Mode lt 0 ov vo9 sade inededa ie vea didnot ees 2 5 2 3 Configuring Instrument Settings lt 2 2 24 e4dcat ecbaasd decd ieetdees eness 2 6 2 3 1 Configuring Basic Instrument Settings 00 00 e ee 2 6 2 3 2 Defining and Editing Filter Slides 00 00 000 eee 2 8 2 3 2 1 Adding Filter Slides ic4 piige eens oS ad a dee wae es 2 8 2 3 2 2 Configuring Filter Slides 24 0 0co0eh4ee ioeesen gue ees 2 9 2 3 2 3 Removing Filter Slides 2254 2540 cease anaana 2 10 2 3 2 4 Exporting and Importing Filter Slides 2 10 2 3 3 Manually Controlling the Instrument 0 0 2 11 2 3 3 1 Temperature Control 880 only 005 2 11 2 3 3 2 Shake Controlar erac tates oo Sea ive ee Vee ass e 2 12 233 3 Plate Control silean okt thee inetd Rete ee adeas 2 12 2 3 3 4 Excitation and Emission Filter Slide Control 2 12 3 Creating and Editing Detection Methods 3 1 OVERVIEW doin d
142. t Settings 0 0 0 00 5 6 5 2 5 Configuring Detection and Preparation Methods 5 8 5 2 5 1 Configuring a Method in Basic Mode 5 9 5 2 5 2 Configuring a Method in Advanced Mode 5 13 5 2 6 Configuring Output Settings 4 ic 0ssiaceeeeddaasvasadas 5 18 5 2 6 1 Configuring a Program to Run after Protocol Executes 5 20 5 3 Running Protocols osaceae sade smackes gatere tedas a aites da teada hetau 5 21 5 3 1 Running a Protocol on an Instrument nosunu nananana 5 21 5 3 1 1 Optimizing Read Height 880 only 5 23 5 3 2 Running a Protocol When Simulation Mode is Enabled 5 26 54 Editing Protocols 24 4 rcncaue ceadde ted tab oeanwedneetat dake tegned 5 28 5 5 Copying Protocols 46h iadvi doi eeta 4 ate dete dees iute seated ees 5 29 5 6 Deleting Protocols sses lt tbwaesdintone treat eoet dh dasdtetadineteae ses 5 30 5 7 Printing Protocol Configuration Information 00 0000 00 00 5 31 5 8 Exporting and Importing Protocols 00 000 eee cece eens 5 33 Multimode Detection Software User s Manual 6 Viewing Measurement Results 6 1 OVGIVIEW otadir naire nagan enn Rehab ae eke serene ERANA anes 6 1 6 2 Viewing Measurement Results in Run Protocol 0 000 eee 6 3 6 2 1 Viewing State ae ag ene Rt Pe ARG WAR Ae WAR eH ES 6 3 G22 Viewing Raw DA cin eas ecues lash ees SER CEA E YaN cared eee 6 5 6 2 3
143. t settings in a protocol 5 6 copying 4 10 creating 4 3 defining dimensions 4 3 defining well information 4 3 editing dimensions 4 8 editing well information 4 8 Beckman Coulter Inc optimizing manually selecting well centers 4 16 overview 4 12 performing the optimization 4 15 preparing for optimization 4 14 selecting detection method 4 13 verifying well centers 4 17 selecting and editing lots 4 9 selecting in protocols 5 5 selection guidelines 4 5 languages installing on a German language operating system 1 6 setting 1 16 linear scan measurements configuring 5 10 viewing graph 6 8 luminescence detection methods configuring 3 7 M measurement results choosing storage directory 1 19 printing 6 13 viewing in Run Protocol 6 3 viewing kinetic graphs 6 6 viewing raw data 6 4 viewing results in Excel 6 9 viewing saved 6 9 viewing scan graphs 6 6 viewing state 6 3 measurement techniques selecting in protocols 5 4 microplate carrier ejecting 2 3 loading 2 3 Multimode Detection Software accessing online help 1 4 configuring communication settings 1 13 configuring software settings 1 15 error codes A 1 installing 1 5 launching 1 2 overview 1 1 removing from host computer 1 9 repairing an installation 1 9 xvii selecting methods advanced mode adding set temperature preparation methods 5 16 configuring a shake preparation method 5 15 configuring set temperature preparation methods 5 16 selecting technique type 5 4
144. the desired measurement cycle The status of each sample measured in the cycle appears in the right pane OR For linear and area scan measurements choose the desired sample The status of each measurement point for the chosen sample appears in the right pane 3 Choose a different cycle or sample to view OR Choose a different results tab to view OR Choose Finish to close Run Protocol Measurement results are saved and or prepared for printing when file and print options are defined in the protocol refer to Section 5 2 6 Configuring Output Settings 6 2 2 Viewing Raw Data Raw Data displays the results for each sample or point measured in every cycle Figure 6 3 Z Run Protocol Kinetic 02 Run Protocol Click Finish to export data Detection PITTI TITTY method s Estimated Time 00 00 08 Measurement Time 00 00 08 State Raw Data Graphs Measurement cycle or sample A B D E F G H Finish Figure 6 3 Raw Data displayed for a kinetic absorbance measurement Beckman Coulter Inc Viewing Measurement Results 6 5 To view Raw Data of samples or measurement points 1 If necessary in the left panel of the tab click the next to the desired detection method to expand the view and access measurement cycles Figure 6 3 2 For absorbance luminescence and fluorescence measurements select the desired measurement cycle Raw data measured for each sample in
145. thod Selection Beckman Coulter Inc Defining Editing and Running Protocols 5 9 5 2 5 1 Configuring a Method in Basic Mode Configuring a method in basic mode includes selecting a detection method and configuring properties for a kinetic or scan measurement and shaking if desired To select and configure a method in basic mode 1 In Method Selection choose Basic to toggle from advanced mode if necessary Basic mode configuration parameters appear Figure 5 8 Refer to Section 5 2 5 2 Configuring a Method in Advanced Mode to configure a method with multiple detection and or preparation methods Create Protocol Kinetic 260 General Settings Technique Type Labware Selection Layout Settings Method Selection Select from available methods below Click Next to proceed Click Back to go back Advanced New Method Output Settings T M et h o d Method Selection Method Properties s gt ABS 260 ha Kinetic Yes Selection Kinetic Cycles 3 Kinetic Interval 29 Shake Yes M eth o d Shake Intensity Medium Shake Interval 5 Pro pe rties Shake Mode Linear i Shake Information about the property gt Determines if shaking will be performed in the kinetic intervals field being configured Figure 5 8 Configuring a method in basic mode 2 In Method Selection select the detection method for use with the protocol Only detection methods of th
146. tings Figure 1 12 Configuring Software Settings 2 Configure the settings in the desired tab s Setting the System Language Section 1 4 2 1 e Configuring Print Settings Section 1 4 2 2 e Choosing Simulated Data Files Section 1 4 2 3 e Choosing the Directory Where Measurement Results are Saved Section 1 4 2 4 Multimode Detection Software User s Manual 1 16 Installing Setting Up and Using the Software 1 4 2 1 Setting the System Language English is set as the default system language when Multimode Detection Software is installed even when German Language Operating System is selected during installation refer to Section 1 3 2 Using the Setup Program to Install the Software The system language may be changed in the Language tab Figure 1 13 85 Multimode Detection Software Connected Fitter Slides Ex 2 Em 1 File Actions Help Software Settings Language print Settings simulated Data Directory Settings Name Culture File German Germany Deutsch Deutschland Apex de DE resources German Austria Deutsch Osterreich Apex de AT resources English United States English United States Apex en US resources We q Software Settings Available languages Figure 1 13 Choosing the software interface language To change the system language 1 Choose the Language tab All available languages are listed with the current system l
147. tion 5 2 6 Configuring Output Settings When no file or printing options are defined measurement results are deleted and may not be recovered Measurement results are exported to the data directory specified in System Settings refer to Section 1 4 2 Configuring Software Settings Exported results may be viewed edited evaluated and printed using Microsoft Excel or other applications compatible with the file format of the exported data Multimode Detection Software does not provide the ability to open view or evaluate exported results This chapter covers Viewing Measurement Results in Run Protocol Section 6 2 Viewing Exported Measurement Results Section 6 3 Printing Measurement Results Section 6 4 Beckman Coulter Inc Viewing Measurement Results 6 3 6 2 Viewing Measurement Results in Run Protocol Measurement results are displayed in a series of tabs within Run Protocol e State displays which samples were measured successfully and which were not because of errors during the measurement refer to Section 6 2 1 Viewing State e Raw Data displays actual values measured For kinetic measurements results are displayed for each measurement cycle For linear and area scan measurements results are displayed for each measurement point refer to Section 6 2 2 Viewing Raw Data e Graphs displays graphs of measurement data for kinetic and scan measurements only r
148. tocols may be viewed but not edited TETRI Labware Information gt Labware Lots Property grid Labware Information B Labware Info Date created 4 4 2004 Date edited 4 4 2004 Plate Name B Labware Measurements Height 1 4 Height with lid 2 222 Length 12 77 Reading height 2 422 width 8 57 E Well Info Columns 12 Rows 8 Well bottom shape Flat Well shape Round Well volume 300 Plate Name Name of this labware Notes Supported techniques Absorbance Luminescence LuminescenceGlow LuminescenceFlash BioLuminescenceRET FluorescenceRET FluorescenceIntensityTop FluorescenceIntensityBottom FluorescencePolarization TimeResolvedFluorescence OOOOOOO0OO8 j Cancel Figure 4 4 Editing labware dimensions and well information To edit labware information In the property grid edit labware dimensions and information as desired Refer to Section 4 2 1 Defining Labware Information for more information about the fields available in the property grid In Supported Techniques change the measurement techniques supported by the labware if desired Note Refer to Section 4 2 1 1 General Labware Selection Guidelines for more information about labware technique compatibility Edit the labware Notes if desired Choose Next to select and edit labware lots and save changes made to the labware refer to Section 4 3 2 Selecting and Editing Labware Lots OR C
149. torage directory is c program files beckman coulter inc multimode detection software data Use Directory Settings to change the storage directory if desired Figure 1 16 85 Multimode Detection Software Connected Filter Slides Ex 2 Em 1 File Actions Help Software Settings Protocols Language Print Settings Simulated Data a Detection Methods a Data Directory 5 Labware c program files beckman coulter inc multimode detection software datal ate WA Instruments Software Settings 5 Browse for desired storage directory Figure 1 16 Choosing the directory where data is saved To choose a different storage directory 1 In Data Directory enter the complete path of the desired storage directory for example C documents measurement results DTX 880 MyResults Proceed to step 4 E Choose Browse for a directory Open appears 2 In Open browse to desired directory Multimode Detection Software User s Manual 1 20 Installing Setting Up and Using the Software 3 Choose Open to select the directory and return to Output Settings OR Choose Cancel to close Open without selecting a new storage directory 4 Choose Apply to set the new storage directory Beckman Coulter Inc Setting Up and Controlling Instruments 2 1 Overview Before defining measurement protocols detection methods and labware or running protocols on an instrument using Multimode Det
150. tribution of this program or any portion of it may result in severe civil or criminal penalties and will be prosecuted to the maximum extent possible under the law Cancel Back Figure 1 5 Setup wizard 7 Choose Next to continue the installation Select Installation Folder appears Figure 1 6 i Multimode Detection Software Select Installation Folder The installer will install Multimode Detection Software to the following folder To install in this folder click Nest To install to a different folder enter it below or click Browse Folder c program files beckman coulter inc multimode detection software Browse Disk Cost Installation Install Multimode Detectio folder f or for anyone who uses this computer Everyone C Just me Cancel lt Back Figure 1 6 Selecting installation options Multimode Detection Software User s Manual 1 8 Installing Setting Up and Using the Software Beckman Coulter Inc 10 11 12 Choose Browse to select a different installation folder for the software if desired Choose Disk Cost to view the free space available on all hard drives installed in the computer if desired Select Everyone to allow all user accounts configured on the computer to access and use the software Everyone should be selected when more than one user will be using the software to perform measurements OR Select Just
151. trument and to choose the orientation of the plate on the microplate carrier Figure 4 8 H Optimizing Labware BetterLabware 96 Prepare Labware Select a Detection Method Insert the labware in landscape orientation into the reader The highlighted wells will be scanned to optimize Prepare the Labware gt the labware Click Next to proceed Optimize r A r Select Plate Orientation Select Center of Left Top Well Eject Plate Carrier Landscape Select Center of Left Bottom Well C Portrait Select Center of Right Top Well Close Plate Carrier Cc Opposite Landscape Select Center of Right Bottom Well 2 Opposite Portrait Verify Well Centers Plate orientation Well A1 is highlighted in red Cancel Figure 4 8 Preparing the labware for optimization To prepare labware for optimization 1 Choose Eject Plate Carrier to move the microplate carrier outside the instrument 2 Place the microplate to be optimized on the plate carrier 3 Choose Close Plate Carrier to load the microplate into the instrument 4 In Select Plate Orientation choose the orientation of the plate on the microplate carrier The selected orientation is displayed graphically to the right of the screen with well A1 highlighted in red 5 Choose Next to start the optimization refer to Section 4 6 3 Performing the Optimization Read The optimization read begins automatically OR Choose Back to select a different detection method r
152. tware Error Codes and Solutions Error Code 417 Error Message Z transport commanded to move to a position greater than maximum travel Recommended Solution Contact a Beckman Coulter Service Engineer 419 LED transport initialization failed Contact a Beckman Coulter Service Engineer 420 Shutter transport initialization failed Contact a Beckman Coulter Service Engineer 421 Z transport initialization failed Contact a Beckman Coulter Service Engineer 423 LED transport lost steps Contact a Beckman Coulter Service Engineer 424 Shutter transport lost steps Contact a Beckman Coulter Service Engineer 425 Z transport lost steps Contact a Beckman Coulter Service Engineer 427 LED transport is not energized Initialize the instrument refer to Section 2 2 3 Initializing the Instrument 428 Shutter transport is not energized Initialize the instrument refer to Section 2 2 3 Initializing the Instrument 429 Z transport is not energized Initialize the instrument refer to Section 2 2 3 Initializing the Instrument 431 LED transport error while positioning Contact a Beckman Coulter Service Engineer 432 Shutter transport error while positioning Contact a Beckman Coulter Service Engineer 433 Z transport error while positioning Contact a Beckman Coulter Service Engineer 440 LED number is out of
153. ut Settings For example horizontal linear scans along the center row may be configured only when Read by row is selected while vertical linear scans along the center column may be configured only when Read by column is selected When Read by well is selected only area scans may be configured xi Select the scan resolution and measurement points to read The type of linear scan that may be configured is determined by which reading direction is configured in Layout Settings When Read by row is selected a horizontal linear scan may be configured When Read by column is selected a vertical linear may be configured When Read by well is selected only area scans may be configured Resolution mm Selected Scan Points 0 2248 Paints x 24 Points Y 1 Well boundary Measurement points Click on the center row or column and drag until the desired number of points is selected OK Cancel Figure 5 10 Configuring a linear scan 14 Choose OK to save the scan configuration and close the Scan Selection Editor OR Choose Cancel to close the Scan Selection Editor without updating changes Beckman Coulter Inc Defining Editing and Running Protocols 5 13 15 Choose Next to configure how measurement results are saved and or printed refer to Section 5 2 6 Configuring Output Settings OR Choose Back to configure the plate layout refer to Section 5 2 4 Configuring Labware Layout Sett
154. xt sensitive online help system This section covers e Launching the Software Section 1 2 1 Using the Software Interface Section 1 2 2 e Accessing Online Help Section 1 2 3 1 2 1 Launching the Software To launch Multimode Detection Software From the Windows Start menu choose Programs gt Beckman Coulter Inc gt Multimode Detection Software gt Multimode Detection Software Multimode Detection Software appears Figure 1 1 Note If Multimode Detection Software is not found in the Start menu the software may have been installed for a single user account on the system instead of all accounts Check with the site system administrator or login to the user account with permission to access the software Refer to Section 1 3 2 Using the Setup Program to Install the Software for more information about installing the software for a single or multiple user accounts 5 Multimode Detection Software Connected Filter Slides Ex 2 Em 1 5 xj File Actions Help Toolbar Protocol Selection List Application Created Last Edited Last Executed Absorbance 340 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 Fluorescein Top 384 Basic Application 1 29 2004 1 29 2004 1 29 2004 Luminecsence 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 Rhodamin Bottom 384 Basic Application 1 29 2004 2 25 2004 1 29 2004 TRF Europium 384 Basic Application 1 29 2004 3 17 2004 1 29 2004 M

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