User Manual TCM 400
Contents
1. P N 7121000 515 P N 7121300 Fig 14 2 Fig 14 3 Bulb Replacement Use only recommended 6V 30W high intensity Halogen bulb Pull open the Lamp socket and pull out the blown bulb using a soft cloth Gently insert the new bulb Do not squeeze or press the bulb too much which can cause damage to the bulb and hand Fit the lamp socket by aligning the guide pins with condenser guide holes Push the lamp socket gently into the transmitted light illuminator Fig 15 16 Fig 16 Flip the main switch to ON i Place the specimen on the stage i Engage the 10X objective in the light path Bring the specimen in focus Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting Adjust the aperture iris diaphragm i Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the brightness Observe Specimen 1 Flip the main switch tol ON as shown in Figure 17 2 Rotating the light intensity adjustment knob in the direction of the arrow increases brightness and rotating knob in the opposite direction decreases brightne2. to clean all surfaces that may have come in contact with the specimen 2 To avoid potential electrical hazards when replacing halogen bulb turn the microscopes main switch to the OFF position and disconnect power cord from wall outlet in advance Whenever you replace your microscope bulb allow lamp socket and bulb to cool before touching Fig 1 Applicable bulb replacement 6V30W Halogen bulb P N EL 455 3 Install microscope on a sturdy level table or bench and avoid any restriction of air vents in the base of the unit Do not place microscope on a flexible surface as this could result in blocking the air vents and cause overheating 4 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 5 When installing the microscope route the power cord away from the microscope frame Should the power cord come in contact with the microscopes base the power cord could melt due to overexposure to heat 6 Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected If the unit is not grounded LABOMED can not warrant electrical safety 7 Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and damage to the microscope 8 After operation of microscope be sure to disconnect power cord from connector socket of the microscope or from the wa
3. a very lightly moistened micro fiber cloth Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose Ff TCM 400 Binocular Lamp Housing Binocular head VO N Stage plate Illumination housing Specimen holder Phase contrast ider Eyepieces Microscope body Illumination intensity control knob So e Va Mains switch js ls Revolving Nosepiece Cen Coarse and fine focusing knob C TCM 400 Trinocular Lamp Housing Trinocular Port Specimen holder Phase contrast slider Microscope body Illumination intensity control knob Mains switch Coarse and fine focusing knob TCM 400 Ergonomic Lamp Housing Ergonomic head Illumination housing Oy Stage plate Specimen S holder Eyepieces 9 Phase contrast slider O 3 aie Microscope body Illumination intensity control knob Mains switch Revolving Nosepiece Coarse and fine focusing knob UNPACKING YOUR MICROSCOPE TCM400 comes securely packed in the custom configured foam packing to prevent it from all shocks and transit damages Make sure that you receive all the following components before disca
4. microscope as this could result in malfunction or mitigated performance 4 When not using the microscope ensure that the frame is fully cooled before storing the unit in a dry locker or covering with a dust cover provided 5 To clean the condenser remove the condenser by rotating it anti closewise Wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue 6 Besure to observe your local rules regulations for product disposal w Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when notin use Optical Cleaning 1 The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage 2 To clean the lens surfaces remove dust using a soft brush or compressed air cans available at your local electronics store For removing finger marks or grease soft cleaning cloth or lens tissue lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use Methanol Observe sufficient caution in handling Methanol Place the ob
5. ocular sleeve and tighten the thumb screw 13 4 1 Fig 7 Allen wrench 3 0mm Base unit is assembled with co axial focus mechanism turret stage plate and the lamp house arm as shown in figure Fig 7 as shown 1 Insert the illuminator into the arm opening and rotate the illuminator 90 clockwise so that the letters NA 0 30 faces directly in front See fig 8 1 2 Tighten the clamping screw using a allen wrench 3 0mm provided with microscope See fig 8 2 14 Phase Slider Filter Head Locking screw Fig 11 1 Hold the phase slider face up LABOMED written side facing upward with the finger on the right and slide it in gently into the slot As shown in Fig 9 Phase slider has click stops at the three positions i e 4x 10x 20x 40x and bright field stop 2 The Blue filter is placed in the filter holder cartridge The cartridge can be easily fitted in the lamp house Hold the cartridge with your index finger and thumb and slide it in See fig 10 1 Loosen the Head Locking screw using allen wrench 2 0mm provided with the microscope to such a position where it does not come out See fig 11 2 Place the observation head on the stand and engage the dovetail ring provided at the bottom into the engaging recess provided on the stand 3 Tighten the head locking screw after rotati
6. 1 Amicroscope is a precision instrument with delicate glass components Please handle with care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations For the operating conditions see chapter 14 SPECIFICATIONS 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body when installing the unit A To prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unit to avoid damage to the specimen slide Maintenance and Storage 1 Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe with cleaning cloth slightly moistened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent Do not disassemble any part of the
7. LABOMED TCM 400 User Manual Inverted Tissue Culture Microscopy To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended CONTENTS INTRODUCTION The TCM 400 inverted microscope sets a new standard for inverted tissue culture microscopes It combines high performance and distinctive styling with the high quality craftsmanship for which Labomed is known The excellent image and comfort of use are the basics of Labomed TCM400 The ergonomics of the design an aesthetically configured TCM400 is the instant choice for IVF ICSI and Culture studies in the era of bio sciences Offered with Infinity Corrected True color optical system choices of observation tubes soft feel turret movement stage extenders micro manipulators giving a paramount performance The user should be aware that the protection provided by the equipment may be impaired if used with accessories not provided or recommended by the manufacturer or used in a manner not specified by the manufacturer SAFETY INFORMATION 1 After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to preventinfection E Be sure to remove the specimen before moving this product E Incase the specimen is damaged by erroneous operation it is important
8. druple positions fixed 4 Observation tube Binocular Trinocular Ergonomic Field number 22 22 22 Tube tilting angle 30 30 O to 25 Interpupillary distance 52 75 52 75 52 75 adjustment range 5 Stage Size 240 x 160mm with mechanical stage Movement range 78 x 54mm Specimen holder Slide and Petri dish 6 Condenser Type Incident condenser daylight filter detachable N A 0 30 Aperture iris diaphragm Built in 7 Dimensions amp Weight 495 0mm L x 300 0mm W x 470mm H 9 5 kg net 8 Electrical Lamp Halogen 6V 30W Lamp life up to 5 hours Input 100V 240V AC 50 60 Hz 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage 100 VAC TO 240 VAC Supply voltage fluctuations Not to exceed 10 of the normal voltage Power consumption 50W Fuse F5A 250V Power supply to the halogen lamp 6V AT 30W Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 CAUTION HIGH VOLTAGE FUSE REPLACEMENT WARNING HIGH VOLTAGE POWER INLET EARTH WARNING HIGH TEMPERATURE D Oppe ee TTT 22 www laboamerica com Our policy is one of continuous development Labo America Inc
9. epieces out from the observation heads ocular tube 2 Insert desired eyepieces in empty ocular tube Fig 3 1 eff L12 INITIAL SETUP amp ASSEMBLY Mounting the Objectives Settle down the nosepiece by rotating coarse adjustment knob towards back Remove the dust prevention caps from the nosepiece Screw the objective with the lowest magnification into the revolving nosepiece from left side of the microscope Turn the nosepiece clockwise and mount the remaining objectives in ascending magnification order It makes easier to change magnification Fig 4 A Clean the objectives periodically A Be sure to cover any unused threaded positions with the dust prevention caps to prevent from dirt and dust getting inside Install the observation head using the following procedure 1 Using allen wrench 3mm provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the Observation Head as desired See figure 5 Insert the eyepieces into the ocular tube of Observation Head using following procedure 1 Remove the protective caps from the observation tube 2 Insert 10x eyepieces into the
10. jectives and or eyepieces on a dust free surface e g aluminum foil All other optical components to be cleaned should be as accessible as possible 3 Blowall loose dust particles away with compressed air or mini dust blower Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab 5 To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol 6 Grease must always be removed using a solvent 7 Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above zig zag motion X spiral motion v Wipe using a spiral movement Do not use a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with
11. ll power outlet Safety Symbols The following code categories describe the degree of danger or damage likely to be incurred in the event of user error made in ignorance of these symbols Symbol Explanation Hot parts symbol A This symbol is placed on bulb housing and on other parts to indicate that they may be hot Never touch those parts just after the equipment has been used Allow sufficient time to cool before touching them In the event of user error it is possible to injury WARNING In the event of user error death or serious injury is possible artention In the event of user error product may be damaged The following describes icon directives This icon denotes Refer to User Manual and Caution This icon represents the productive earth This icon represents alternating current O This icon represents the power switch is turned OFF This icon represents the power switch is turned ON Labeling Infromation SN Number following this symbol indicate the serial number of the equipment REF Character following the symbol indicate the catalog number of the equipment A Caution If the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipments as outlined in this instruction manual Getting Started
12. m entering into your line of vision 1 2 TROUBLESHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take remedial action as needed In case of any other problem which cannot be rectified as below please contact the service agents Trouble Cause Remedy 1 Although the illumination is on the field of view is dark The socket pin is not connected to the illumination column at the back of the instrument The bulb is burned out The light intensity control is set to too low The objective is not in position Fuse is blown Connect it securely Replace the bulb Set the illumination to desired position Make sure the objective is clicked properly Replace fuses 250V 5 Amps 2 Dirt or dust is visible in the field of view Dirt dust on the specimen Dirt dust on the eyepieces Clean the specimen Clean them thoroughly 3 Visibility is poor Image is not sharp Contrast is not sharp Details are indistinct Phase contrast effect cannot be obtained The objective is not correctly engaged The aperture iris diaphragm is opened or stopped down too far in brightfield observation The light annulus of the condenser does not match the phase annulus and phase Replace it with a clean specimen Clean eyepieces gently Adjust the aperture O
13. ng the Binocular Head to your preffered direction of use 15 mmoommnnmooooo Adjusting the Interpupillary Distance and Diopter While looking through the eyepieces move both eyepieces until the left and right fields of view coincide completely This matches your IPD Fig 12 Adjusting the Diopter Adjust the Diopter setting to 0 on the left side eyepiece While looking through the left eyepiece with your left eye turn the coarse and fine focus adjustment knobs to bring the specimen into focus Adjust the Diopter setting to 0 on the right side eyepiece and while looking through the right eyepiece with your right eye turn only the diopter adjustment ring to focus on the specimen See figures 12 and13 Diopter ring Fig 13 qi Mounting the stage Extension amp Mechanical stage The stage extension plate can be mounted on the left or right side or both sides of the stage to expand the stage surface However the stage extension plate and mechanical stage cannot be used simultaneously on the same side Stage Extension Plate Screw the studded clamping screw into the plain stage from Underneath Tighten them with hand until the plate is securely attached Refer fig 14 1 and 14 2 Mechanical Stage This can be attached on either side of the stage Attach in the same way as the stage extension plate Refer to fig 14 3 oe
14. rding packing material Microscope body Observation tubes as ordered Eyepieces Illumination housing Stage extension plates Specimen plate Phase slider Phase telescope Objectives Blue filter Green Filter Frosted filter Installation tools Power cord Spare fuses as ordered Spare bulb as ordered Adapter for telescope Dust cover 1S oS pe nS Soe Fe oe of STANDARD COMPONENTS After removing your microscope from its packaging make sure that all of the following contents are present Please note that the contents of your microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highlighted here ST _ _ So ae Ne Daylight Blue filter Green filter Frosted filter Paired Eyepieces m Allen Wrench 3mm Phase Telescope with 6V 30W Halogen bulb adapter 10 OPTIONAL ACCESSORIES System Diagram of Optional Accessories Installation and Operation of Optional Accessories iVu Camera Module System 1 Mount the Video adapter 1 2 part 3143300 712 on Trinocular observation head 2 Mount iVu Camera Module System on video adapter 11 Optional Eyepieces 10X eyepieces are provided To replace 1 Pull out the 10x ey
15. reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 445 1317 CA 94538 sales laboamerica com LABOMED and TCM 400 are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2009 Labo America Inc 7125000 990A 12 2009 CE CA G uS ISO 9001 2000 File No A9020 Printed in U S A
16. ss The intensity bar on the knob indicates the direction of intensity level Fig 17 Placing specimen on the stage Place the specimen on the stage plate In case the specimen is in Petri dish keep it on the petri dish holder In case you are using high Flask remove the condenser front lens by rotating it anti clock wise to fit in the flask As shown fig 18 E In case your microscope has mechanical stage mounted and you are using either glass slide or 90 well plate use appropriate holders cen ZI I T Fig 18 Adjusting the Focus Focusing Procedure Figure 1 9 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recommend starting with 10X 2 While observing the specimen through the eyepieces slowly rotate the coarse adjustment knob 1 counterclockwise to lower the objectives 3 When coarse focusing of the specimen is obtained an image is observed rotate the fine adjustment knob 2 for fine detail focusing Working Distance WD The WD refers to the distance between each objective and the specimen when acute focus of the specimen is obtained Objective Magnification 4X 10X 20X 40X WD mm 16 7 2 5 35 2 0 19 Adjus
17. ting the Interpupillary Distance IPD Fig 20 Adjusting the Diopter Fig 22 The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly referred to in the future This is happen when multiple users work with the microscope Adjust the Diopter setting to 0 on the left side eyepiece While looking through the left eyepiece with your left eye turn the coarse and fine focus adjustment knobs to bring the specimen into focus Adjust the Diopter setting to 0 on the right side eyepiece and while looking through the right eyepiece with your right eye turn only the diopter adjustment ring to focus on the specimen Using the Eye Guards When Wearing Eyeglasses Use with the eye guards in the normal folded down position This will prevent the eyeglasses from being scratched When Not Wearing Eyeglasses Extend the folded eye guards outwards direction of the arrow to prevent ambient light fro
18. verlap adjust Phase annuli position 4 One side of image is blurred The revolving nosepiece is not correctly engaged The specimen is not correctly mounted on the stage The optical performance of the culture vessel bottom plate is poor Make sure that the revolving nosepiece clicks properly into place Place it correctly on the stage Use a vessel with a good profile irregularity characteristic 5 The bulb flickers and the brightness is unstable The line voltage fluctuates The bulb is nearly burned out The power cord is not connecting securely Use a voltage stabilizer Replace the bulb Connect it securely 6 The coarse adjustment knob is too difficult to rotate The tension adjustment ring is tightened too much The tension adjustment ring is loosened too much Loosen it appropriately Tighten it appropriately 7 The field of view of one eye does not match that of the other Incorrect interpupilary distance adjustment Incorrect diopter adjustment Adjust the interpupilary distance Adjust the diopter rA TF 1 Illumination Built in illumination system Halogen 2 Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 0 002 per graduation Fine adjustment stroke 0 2mm per turn Total stroke 28mm Co axial coarse and fine focusing on ball drive Coarse adjustment travel per rotation 3 Revolving nosepiece Qua
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