Home

CircuLex TM CircuLex TM

image

Contents

1. deviations to the mean optical density value of twenty standard replicates and calculating the corresponding concentration A Typical standard curve Q X Human PCSK9 Standard Curve 3 0 2 5 2 0 15 lt 1 0 0 5 0 0 4 m 0 2 4 6 8 10 Q PCSK conc ng ml o e Q m CY 8079 10 Versions 151105 LS d o S i Pu Human PCSK9 ELISA Kit re CirculLlex User s Manual For Research Use Only Not for use in diagnostic procedures m E A Intra assay Precision Precision within an assay Three samples of known concentration were tested sixteen times on one plate to assessrajwra assay precision Intra assay Within Run n 16 CVz1 5 2 6 96 g Human PCSK9 conc ng mL c o m CY 8079 11 Versions 151105 LS c L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures d Human PCSK9 ELISA Kit Inter assay Precision Precision between assays Three samples of known concentration were tested in five separate assays to assess in rassay precision S nter assay Run to Run n 5 CV 2 90 7 09 96 Qo Human PCSK9conc ng ml amp Serum 1 Serum 2 Serum 3 Y 1 81 7 259 2 3495 2 Y 2 68 9 252 2 357 REN 3 77 6 266 3 SARI 4 80 8 251 2 1 5 5 72 9 247 8 S MAX 81 7 266 3 S 3781 8 MIN 68 9 E 3495 2 MEAN 76 4 255 3633 9 S D 5 419 zf 107 931 C V 7 09 2 90 2 97 w 3 Spiking Recover xO Serum samples were spiked with different gmgo
2. d Human PCSK9 ELISA Kit CircuLex secu Manual For Research Use Only Not for use in diagnostic procedures gt ELISA Kit for Measuring Human PCSK9 3 CircuLex Human PCSK9 ELISA KiS Cat CY 8079 Qo Intended Use eee 1 od sr lr M 1 Y Introduction eese 2 QN Principle of the Assay erepta 2 3 a Materials Provided usse 3 Yon Materials Required but not Provided 4 S Precautions and Recommendations 5 N Sample Collection and Storage 6 amp Detailed Protocol eee 7 8 amp Calculations eeeeeeen 9 Q Measurement Range eese 9 o TroubleshoOBng ise entorno rtt reine 9 gt Reagent Stability 10 Assay Characteristlos ues ceo canat eonim 1 Example of Test Results S Referenc s eere tesete cec Ee Ibo soot eee e eiin 7 Related Products esee e 18 Intended Use e The CycLex Research Product CirgnLex Human PCSK9 ELISA Kit is used for the quantitative measurement of Human PCSK9 in Gym plasma cell culture medium and other biological media This assay kit is for researcha only and not for use in diagnostic or therapeutic procedures e Storage Upon receipt store ponents at 4 C Don t expose reage to excessive light gt o e Q m CY 8079 1 Versions 151105 LS d Human PCSK9 ELISA Kit
3. n CircuLex secu Manual d Human PCSK9 ELISA Kit For Research Use Only Not for use in diagnostic procedures amp Materials Required but not Provided 9 Pipettors 2 20 uL 20 200 uL and 200 1000 uL precision pipettors with disposable tips RS N Precision repeating pipettor KS Orbital microplate shaker g e Microcentrifuge and tubes for sample preparation c Vortex mixer e Optional Microplate washer Manual washing is possible but not proggie e Plate reader capable of measuring absorbance in 96 well plates aD wavelengths of 450 nm 540 nm Dual wavelengths of 450 550 or 450 595 nm can also be usi The plate can also be read at a single wavelength of 450 nm which will give a somewhat eon Optional Software package facilitating data generation qu analysis 500 or 1000 mL graduated cylinder K Reagent reservoirs Q Deionized water of the highest quality wW o Disposable paper towels o m CY 8079 4 Versions 151105 LS d CircuLex secu Manual n d Human PCSK9 ELISA Kit For Research Use Only Not for use in diagnostic procedures NEM Precautions and Recommendations AS All microplate strips that are not immediately required should be returned to the zip lock pouch which must be carefully resealed to avoid moisture absorption Q S Do not use kit components beyond the indicated kit expiration date n Allow all the components to come to ro
4. L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures amp n C Q Introduction 9 PCSK9 also known as neural apoptosis regulated convertase NARC 1 is a 692 residue e llular protein expressed primarily in the kidneys liver and intestines 1 representing the 9th mae of the secretory subtilase family Various genetic observations subsequently mapped PCSK9 as hird gene along with LDLR and APOB to cause autosomal dominant hypercholesterolemia studies suggested that gain of function mutations increase plasma levels of LDL nonsense or missense loss of function mutations which interfere with folding or lead to a reduction of plasma levels of LDL c and an 88 decrease in the risk of nary heart disease CHD 5 In mice adenoviral overexpression of PCSK9 results in increasedyplasma LDL c level in normal mice but not in LDLR deficient mice 7 Deletion of PCSK9 causeffn increase in level of LDLR protein but not mRNA 8 These findings lead to a hypothesis t CSK9 exerts its role in cholesterol metabolism through posttranslational down regulation of LDR fhe receptor responsible for clearing LDL c from plasma Evidence is consistent with the secreted form of PCSK9 binding direy to the LDLR and resulting in degradation of the receptor 9 10 Zhang et al 11 localized the binging site of PCSK9 in the LDLR to the first epidermal growth factor like repeat EGF A of the rere domain and showed that PC
5. 151175 MEINEN E Pp 150 e E E e voco EEEEEEEEEEEENN 126 150 s v i 9 lt 10 125 is 2 i i 8 L W 100 5 D n lt 76 100 MEN o e 51 75 NN Y F 50 7 25 50 i 24 0 0 4 8 12 Male Female e e Q m CY 8079 14 Versions 151105 Q amp C L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures d Human PCSK9 ELISA Kit Fig 2 Stability of PCSK9 in Human Serum 3 Stability of PCSK9 in Human Serum Y 400 de37 C 4 4 20 C a 80 C E a 3 5 200 o Oy x n o amp 100 0 0 10 20 30 40 50 60 Strage time Day Ww Fig 3 PCSK9 conc in various conditioned mein O PCSK9 conc in various conditioned media 50 E 40 oD amp g 30 8 g 20 n z 10 o HE m MRC5 SW480 MCF7 Hela HepG2 A431 KATOIII Culture Q Medium amp Cell line c o m CY 8079 15 Versions 151105 Q amp C nd e CircuLex Human PCSK9 ELISA Kit User s Manual For Research Use Only Not for use in diagnostic procedures Fig 4 Effect of Berberine on secretion of PCSK9 in A431 and SW480 Effect of Berberine on secretion of PCSK9 in A431 7 5 E 25 0 o S 2 2 5 IBI Ul i i amp T Control 10 ug ml 20 ug ml 50 ug ml 10uM 20uM 50uM Berberine Monensin NJ Effect of Berberine on secretion of PCS
6. T K M A Berge K E and Leren T P 2006 Hum Mol Genet 15 1551 1558 amp 11 Zhang D W Lagace T A Garuti hao Z McDonald M Horton J D Cohen J C and Hobbs H H 2007 J Biol Chem 18602 18612 e y ge S O e O S Q e Q qu CY 8079 17 Versions 151105 LS c Human PCSK9 ELISA Kit L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures no A C Q amp Related Products CircuLex Mouse Rat PCSK9 ELISA Kit Cat CY 8078 AS CircuLex Human PCSK9 ELISA Kit Cat CY 8079 NS CircuLex PCSK9 LDLR in vitro Binding Assay Kit Cat CY 8150 S Anti Human PCSK9 prodomain monoclonal antibody KS 3C8 Cat CY M1032Q Anti Human PCSK9 monoclonal antibody KS 4H12 Cat CY M1033 amp PCSK9 Wild Type in culture medium Cat CY R2310 Y PCSK9 A 33 53 Wild Type in culture medium Cat CY R2320 PCSK9 D374Y in culture medium Cat CY R2311 X PCSK9 A 33 53 D374Y in culture medium Cat CY R2321 Yon PCSK9 Wild Type A53 in culture medium Cat CY R2320 QN PCSK9 Wild Type Cat CY R2330 a PCSK9 D374Y Cat CY R2331 Yon PCSK9 R194A Cat CY R2333 wy LDLR EGF AB domain Cat CY R2340 amp LDLR EGF AB domain Myc tagged Cat CY R2341 m LDLR EGF AB domain H306Y Cat CY R2342 Y LDLR EGF AB domain H306Y Myc tagged Cat CY RQ3 PRODUCED BY amp CycLex Co Ltd y 1063 103 Terasawaoka Q Ina Nagan
7. dilution factor R 1 The dose response curve of this assay fits best to a sigmoidal ESA logistic equation The results of unknown samples can be calculated with any computer grogfam having a 5 parameter logistic function It is important to make an appropriate mathematical istment to accommodate for the dilution factor wy 2 Most microtiter plate readers perform automatic calculations of flyte concentration The calibration curve is constructed by plotting the absorbance Y of bros versus log of the known concentration X of calibrators using the four parameter function Alternatively the logit log function can be used to linearize the calibration curve 14 amp logit of absorbance Y is plotted versus log of the known concentration X of calibrators Measurement Range Q The measurement range is 0 16 ng mL to 10 aL Any sample reading higher than the highest standard should be diluted with Dilution Buff igher dilution and re assayed Dilution factors need to be taken into consideration in calculating thg human PCSK9 concentration Troubleshooting s 1 All samples and standards should b assayed in duplicate using the protocol described in the Detailed Protocol Incubation times or peratures significantly different from those specified may give erroneous results Q 2 Poor duplicates accompaff lj by elevated values for wells containing no sample indicate insufficient washing If all instructiQghs in the Detailed Protocol were
8. Add 100 uL of HRP conjugated Detection Antibody into each on Incubate the plate at room temperature ca 25 C for 1 ng skin at ca 300 rpm on an orbital microplate shaker Q Wash 4 times by filling each well with Wash Bufeo uL using a squirt bottle multi channel pipette manifold dispenser or microplate washer 9 Add 100 uL of Substrate Reagent Avoid MNN the microtiter plate to direct sunlight Covering the plate with e g aluminum foil is recommeng y Return Substrate Reagent to 4 C immediately after the necessary volume is removed amp Incubate the plate at room temperatu 25 C for 10 20 minutes shaking at ca 300 rpm on an orbital microplate shaker The incubyon time may be extended up to 30 minutes if the reaction temperature is below than 20 C Add 100 uL of Stop Solution each well in the same order as the previously added Substrate Reagent 12 Measure absorbance in M using a spectrophotometric microplate reader at dual wavelengths of 450 540 nm Dual waveJtagths of 450 550 or 450 595 nm can also be used Read the microplate at 450 nm if only a sin velength can be used Wells must be read within 30 minutes of adding the Stop Solution Note 1 Compleje removal of liquid at each step is essential to good performance After the last wash remo ny remaining Wash Buffer by aspirating or decanting Invert the plate and blot it agai clean paper towels Note 2 R le standard curves are obtained when either
9. K9 in SWA80 1 5 iL PCSK9 conc ng ml Nn 0 0 Control 10 n 20 ug ml 50 ug ml 10uM 20uM 50uM Berberine Monensin Oo S Q e n S ihi Human PCSK9 ELISA Kit e Circu Lex User s Manual A For Research Use Only Not for use in diagnostic procedures F References 9 Seidah NG Benjannet S Wickham L Marcinkiewicz J Jasmin SB Stifani S Basak QQ rat A Chretien M 2003 Proc Natl Acad Sci USA 100 928 933 S j N Abifadel M Varret M Rabes JP Allard D Ouguerram K Devillers M Cruaud Q Benjannet S Wickham L Erlich D et al 2003 Nat Genet 34 154 156 amp uo Leren TP 2004 Clin Genet 65 419 422 4 Allard D Amsellem S Abifadel M Trillard M Devillers M Luc G Krempf QV Reznik Y Girardet JP Fredenrich A et al 2005 Hum Mutat 26 497 SC Nn Cohen JC Boerwinkle E Mosley TH Jr Hobbs HH 2006 N Engl J MY 354 1264 1272 6 Berge KE Ose L Leren TP 2006 Arterioscler Thromb Vasc Biol 48910941100 7 Maxwell KN Breslow JL 2004 Proc Natl Acad Sci USA 10140007105 8 Rashid S Curtis DE Garuti R Anderson NN Bashmakov Ho YK Hammer RE Moon YA Horton JD 2005 Proc Natl Acad Sci USA 102 5374 5379 K 9 Lagace T A Curtis D E Garuti R McNutt M G Park S W Prather H B Anderson N N Ho Y K Hammer R E and Horton J D 20 Clin Investig 116 2995 3005 10 Cameron J Holla O L Ranheim
10. O D values do not exceed 0 25 units for the zero concentration or 3 0 units for the highest standard concentration The plate ould be monitored at 5 minute intervals for approximately 30 minutes Note 39 If the microplate reader is not capable of reading absorbance greater than the absorbance of the highest standard perform a second reading at 405 nm A new standard curve constructed e using the values measured at 405 nm is used to determine PCSK9 concentration of off scale Q samples The readings at 405 nm should not replace the on scale readings at 450 nm m CY 8079 8 Versions 151105 LS d Human PCSK9 ELISA Kit L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures amp n C Q Calculations 3 Average the duplicate readings for each standard control and sample and subtract the av Zero standard optical density Plot the optical density for the standards versus the concentr of the standards and draw the best curve The data can be linearized by using log log paper EC vii analysis may be applied to the log transformation To determine the PCSK9 concenfygtion of each sample first find the absorbance value on the y axis and extend a horizontal line to tb amp standard curve At the point of intersection extend a vertical line to the x axis and read the co onding PCSK9 concentration If the samples have been diluted the concentration read from the st nffard curve must be multiplied by the
11. SK9 binding to this site is required for LDLR degradation I t of these observations and the fact that PCSK9 in the circulation may cause the degradation of kep tic LDLR in the liver PCSK9 would seem to be an attractive drug target for lowering LDL c Q Principle of the Assay 3 The CircuLex Human PCSK9 ELISA Kit empl the quantitative sandwich enzyme immunoassay technique An antibody specific for PCSK9 has been pre coated onto a microplate Standards and samples are pipetted into the wells and the werovilized antibody binds any PCSK9 present After washing away any unbound substances an HRP conjugated monoclonal antibody specific for PCSK9 is added to the wells Following a wash to ve any unbound antibody HRP conjugate the remaining conjugate is allowed to react with the see H2O tetramethylbenzidine The reaction is stopped by addition of acidic solution and absorbar of the resulting yellow product is measured at 450 nm The absorbance is proportional to the conggntration of PCSK9 A standard curve is constructed by plotting absorbance values versus PCSK9 centrations of calibrators and concentrations of unknown samples are determined using this standard irve The CircuLex Human PCS ISA Kit is designed to measure the concentration of Human PCSK9 from human serum plasma o Qonditioned medium NI S oO e O S Q e m CY 8079 2 Versions 151105 o d CircuLex secu Manual For Research Use Only Not for use in diagnostic proc
12. cedures amp n C Q Sample Collection and Storage 9 Serum Use a serum separator tube and allow samples to clot for 60 30 minutes Copa ze the samples at 4 C for 10 minutes at 1 000 x g Remove serum and assay immediately or storages on ice for up to 6 hours before assaying Aliquots of serum may also be stored at below 70 r extended periods of time Avoid repeated freeze thaw cycles Plasma Collect plasma using EDTA Naz as the anticoagulant If possible colle e plasma into a mixture of EDTA Naz and Futhan5 to stabilize the sample against spontaneous vitro complement activation Immediately centrifuge samples at 4 C for 15 minutes at 1 000 x Assay immediately or store samples on ice for up to 6 hours before assaying Aliquots of plasma lso be stored at below 70 C for extended periods of time Avoid repeated freeze thaw cycles QN Note Citrate plasma has not been validated for use in this assay SS Other biological samples Remove any particulates by centrifugati nd assay immediately or aliquot and store samples at below 70 C Avoid repeated freeze thaw cy o m CY 8079 6 Versions 151105 LS c Human PCSK9 ELISA Kit L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures amp n C Q Detailed Protocol 9 The CycLex Research Product CirceuLex Human PCSK9 ELISA Kit is provided with vable strips of wells so the assay can be carried out on separate occasions using onl
13. edures d Human PCSK9 ELISA Kit gt Summary of Procedure 3 Add 100 uL of diluted sample to the wells AS i Incubate for 1 hour at room temp KS Wash the wells Q S Add 100 uL of HRP conjugated anti PCSK9 monoclonal Gtibody 1 Incubate for 1hour at room temp Y Wash the wells C gt Add 100 uL of Substrate Reagent 4 Incubate for 10 to 20 fides at room temp Add 100 uL of Stop Solution amp i Measure absorbance at 450 nm KG CA Materials Provided e All samples and standards should be assayeM aplicate The following components are supplied and are sufficient for the one 96 well microplate Microplate One microplate supplied re A use with 96 wells 12 strips of 8 wells in a foil zip lock bag with a desiccant pack Wells are coated with anti PCSK9 antibody as a capture antibody 10X Wash Buffer One 100 mL bf of 10X buffer containing Tween 20 Dilution Buffer One bottle ning 50 mL of 1X buffer use for sample dilution Ready to use PCSK9 Standard One vigheontaining 50 ng of lyophilized recombinant human PCSK9 N HRP conjugated Det Qton Antibody One vial containing 12 mL of HRP horseradish peroxidase conjugated anti PC monoclonal antibody KS 2C10 Ready to use Substrate Reago One bottle containing 20 mL of the chromogenic substrate tetra methylbenzidine TMB Ready se Stop souton bottle containing 20 mL of 1 N H2S01 Ready to use Q S Q m CY 8079 3 Versions 151105 LS c
14. followed accurately such results indicate a need for washer mai ce 3 Overall low signd ay indicate that desiccation of the plate has occurred between the final wash and addition of Substrate Reagent Do not allow the plate to dry out Add Substrate Reagent immediately after wash o S gt Q amp A m CY 8079 9 Versions 151105 LS d Human PCSK9 ELISA Kit L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures amp n C Q Reagent Stability 9 All of the reagents included in the CycLex Research Product CircuLex Human PCSK9 l A Kit have been tested for stability Reagents should not be used beyond the stated expiration qafe Upon receipt kit reagents should be stored at 4 C except the reconstituted PCSK9 Standard mu stored at below 70 C Coated assay plates should be stored in the original foil bag sealed by tly zip lock and containing a desiccant pack a Assay Characteristics Q 1 Sensitivity y The limit of detection defined as such a concentration of PCSK9 giving ifta higher than mean absorbance of blank plus three standard deviations of the absorbance of Blank A blank 3SD blank is better than 0 154 ng ml of sample S3 Dilution Buffer was pipetted into blank wells qe Eighty assays were evaluated and the minimum detectable do DD of PCSK9 ranged from 0 138 0 166 ng mL The mean MDD was 0 154 ng mL The MDD yas determined by adding three standard
15. o 396 0002 Q Japan N Fax 81 265 76 7618 E mail info cyclex c URL http www c CO j CycLex CircuLexproducts are supplied for research use only CycLex CircuLex products and components thebeof may not be resold modified for resale or used to manufacture commercial products with pt prior written approval from CycLex Co Ltd To inquire about licensing for such commggial use please contact us via email gt o e Q qu CY 8079 18 Versions 151105 LS d
16. of Std 11 25 ng ml 300 uL 0 63 ng mL Std 6 300 uL of 0 63 ng ml 300 uL 0 31 ng mL Std 7 300 Lg 6 0 31 ng ml 300 uL 0 16 ng mL Blank 300 uL 0 ng mL Note Do not use a renting pipette Change tips for every dilution Wet tip with Dilution Buffer before disp Ge Unused portions of Master Standards should be aliquoted and stored at below 70 C myy 1ately Avoid multiple freeze and thaw cycles Sample bd Seru plasma samples may require a 100 fold dilution e L serum sample 297 uL Dilution Buffer ge biological samples require neat to appropriate dilution oe Roa CY 8079 7 Versions 151105 o d Assay Procedure paa w n N oo 10 Human PCSK9 ELISA Kit L ircuLex User s Manual For Research Use Only Not for use in diagnostic procedures Remove the appropriate number of microtiter wells from the foil pouch and place them ingee well holder Return any unused wells to the foil pouch refold seal with tape and store at 4 C Q 2 Dilute samples with Dilution Buffer See Sample Preparation above Q Pipette 100 uL of Standard Solutions Std1 Std7 Blank and diluted sample duplicates into the appropriate wells d 4 Incubate the plate at room temperature ca 25 C for 1 hour shaking at EN rpm on an orbital microplate shaker C Wash 4 times by filling each well with Wash Buffer 350 uL using squirt bottle multi channel pipette manifold dispenser or microplate washer 6
17. om temperature before use Use only the microtiter wells provided with the kit e Rinse all detergent residue from glassware C Use deionized water of the highest quality Ro Do not mix reagents from different kits qe The buffers and reagents in this kit may contain preservatives Gu chemicals Care should be taken to avoid direct contact with these reagents Do not mouth pipette or ingest any of the reagents K Do not smoke eat or drink when performing ptr or in areas where samples or reagents are handled x Dispose of tetra methylbenzidine TMB conf solutions in compliance with local regulations Avoid contact with the acidic Stop SolutigGind Substrate Solution which contains hydrogen peroxide Wear gloves and eye protection whet Zadting immunodiagnostic materials and samples of human origin and these reagents In case ontact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and se amp lgmedical attention when necessary Biological samples may b taminated with infectious agents Do not ingest expose to open wounds or breathe aeros ear protective gloves and dispose of biological samples properly CAUTION Sulfuri Asia is a strong acid Wear disposable gloves and eye protection when handig Stop Solution o o S g o m CY 8079 5 Versions 151105 LS d Human PCSK9 ELISA Kit CircuLex secu Manual For Research Use Only Not for use in diagnostic pro
18. unts of PCSK9 and assayed The recovery of PCSK9 spiked to levels t hout the range of the assay was evaluated Sample Average 96 Recovery Range vd serum n 3 82 25 112 05 96 Serum 1 amp TAY None 00 ngmi 100 nem 30ngi Reve oe 98 91 11205 T 107 83 Serum 2 None _ 300 ng ml 100 ng ml 30 ng ml ptecoveryeate ooa 9083 ws Roa CY 8079 12 Versions 151105 Q amp C CircuLex secu Manual For Research Use Only Not for use in diagnostic procedures S Human PCSK9 ELISA Kit 4 Linearity To assess the linearity of the assay samples containing and or spiked with high concentratiOM of PCSK9 were serially diluted with the Dilution Buffer to produce samples with values wifhin the dynamic range of the assay Wh Q C Linearity 5 Serum 1 ri 4 e Serum 2 Sh E a Serum 3 5 E 3 eo e 2 z E E 1 0 0 0 25 0 5 0 75 1 Serum Dilution Ratio x 1 100 KY e Q o Ay q e e Q m CY 8079 13 Versions 151105 Q amp C n S i Human PCSK9 ELISA Kit e Circu Lex User s Manual A For Research Use Only Not for use in diagnostic procedures F Example of Test Results 9 Fig 1 Concentrations of PCSK9 in healthy Japanese volunteers sera n 37 Male 21 Female V e 250 e 201 ae e e 176 200 B e L a
19. y the numb Of strips required for the particular determination Since experimental conditions may vary an WM of the PCSK9 Standard within the kit should be included in each assay as a calibrator Disposqple pipette tips and reagent troughs should be used for all liquid transfers to avoid a OE reagents or samples Y Preparation of Working Solutions All reagents need to be brought to room temperature prior to the assay A D reagents are supplied ready to use with the exception of 10X Wash Buffer and PCSK9 Standard 1 Prepare a working solution of Wash Buffer by adding 100 mL of the X Wash Buffer to 900 mL of deionized distilled water ddH2O Mix well Store at 4 C for two weeks or 20 C for long term storage amp 2 Reconstitute PCSK9 Standard with 0 5 mL of ddH O ThaShcentration of the human PCSK9 in vial should be 100 ng mL which is referred as a Master Stan ard of human PCSK9 Prepare Standard Solutions as follows o Use the Master Standard to produce a dilution N below Mix each tube thoroughly before the next transfer The 10 ng mL standard St serves as the highest standard The Dilution Buffer serves as the zero standard Blank Y Volume of Standar Dilution Buffer Concentration Std 1 60 uL of Master Stand 540 uL 10 ng mL Std 2 300 uL of Std 1 10 D 300 uL 5 ng mL Std 3 300 uL of Std 2 5 D 300 uL 2 5 ng mL Std 4 300 uL of Std 3 ng ml 300 uL 1 25 ng mL Std 5 300 uL

Download Pdf Manuals

image

Related Search

Related Contents

MB556 User Manual - Stanley Hydraulic Tools  iPR-8 proximity reader User manual  手続きの流れとQ&A(PDF:100.6KB)  REX 配水用 EF コン トロ  Food Protection Mobile  Mode d`emploi - Circonscription Montpellier Sud  Chief FCA106 mounting kit  RC Lesen Komplett  "user manual"  S11.5 Indoor Cycle  

Copyright © All rights reserved.
Failed to retrieve file