Locator and Locator Plus User Manual
Contents
1. Locator and Locator Plus Cryogenic Storage Vessel with or without Ultrasonic Level Monitor Operation Manual and Parts List LT509X9 7018141 Rev 2 Visit us online to register your warranty Thermo www thermoscientific com labwarranty SCIENTIFIC Preface Locator Plus Locator Plus Catalog Number Fi CY50925 70 w o Monitor CY50935 70 w o Monitor Locator Locator Catalog Number m Inne CY50925 w o Monitor CY50935 w o Monitor CY50985 70 w o Monitor Locator 6 Plus CY50945 70 w o Monitor Locator 8 Plus CY509106 w Monitor 8237 CY50945 w o Monitor 105 w Monitor 105CN w Monitor 107 w Monitor 107CN w Monitor 113 w Monitor 110 w Monitor 110CN w Monitor CY50985 w o Monitor CY509106CN w Monitor CY509108 w Monitor CY509108CN w Monitor CY509109 w Monitor CY509109CN w Monitor 8210 Locator Plus China ES 8210 L CY509111 w Monitor 8713 CY509111CN w Monitor 8214 Locator 8 Plus China MANUAL NUMBER LT509X9 7018141 2 31623 5 15 15 Added caution for moving vessel in Section 3 CCS 1 31528 9 15 14 Updated conformity specs and declaration CCS 0 27564 1 31 14 Original new PCBA design CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Cryogenic Storage Vessel i Preface Cryogenic Storage Vessel A CAUTION Contains Parts and Assemblies M za Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to re2. 91 1L 91 GLE91 60 91 L0 91 Aiddng SE d 2 NOZX60SNO NOSLX60NO NOSLX60SNO NOBLXBOSNI NOBLXBOSNI NOLIXBOSND NISEXBOSNI iuo yum Ger 191 v0 91 v0 91 9 91 t 91 80 91 goegLe Alddng samog pue X603N GLX60SN9 GLX608N2 0 L1X60SN2 LLX60SN2 0 91X608N2 9LXBOGNI J01IUOJA YIM 18409 8 d01V201 SN 9 H01V201 9401V901 SNId t d01V201 bt d01V3901 SNId Hr d01V901 HF H01V201 jspoyy Ag 1517 Sueg Lt age Thermo Scientific Cryogenic Storage Vessel 1 2 ECETES DEt 1006 OSI OL S 0 ASX uoneuuoJul AJUBIIEM JO JOJNQIASIP 890 Jno 19e3u09 YSN sy episjino uonesildde jeloeds pue asinas esueuajuiewu uoneledo AueJew jueudinbe uo suonsenb noA Jamsue o peal 919M 9 t 0t4 L Jo epeueo pue VSN 158y 8 y 008 1 1e jueuniedeg sadas jeo1uyos INOA jeo aseajd peuinbeJ s anales jueuudinbe y eoueuojureui eAnueAeJd pue uonejedo uonejejsur 3ueuudinbo rejep AjjnJoued sjenueuJ uononujsur pejulud SOALIE jueuudinbe unoA e40Jeq uomneuuoJur uonejedoeJd ous eAisueueJdujoo uj dj y oj Apes si au sejes ows y 890 INOA SsjyonpoJd JO sso Jo SHJOJA SO 0 Dune sabewep uoneyu noyym HBulpnjoul sebewep jenuenbesuoo Jo apu Aue 104 ejqei eq Jou jeus oway AlddV TIVHS ISOdYNd YVINILLAVA V 404 SSANLIS YO ALETISVENVHOSSIN JO SSILNVYYVM ON Oaridigi YO 1V3O NALLIYM Y3HLIHM SSILNVYYVM Y3HLO T1V JO NAN NI ANY 3AISQT19X3 SI ALNVYYVM SI
3. Form Type 1 form B Load Voltage Maximum 24VDC Max Load Current Maximum 120mA Typical On Resistance Maximum 26 Q 6 2 Cryogenic Storage Vessel Thermo Scientific Section 6 LN2 Level Monitor Operation REMOVE 3 SCREWS AND PULL COVER UP AND OUT OF THE WAY REMOVE COVER ANI INSERT WIRES CONNECT TO ur CONNECT TO Figure 6 1 Connect to Remote Alarm Remote Alarm wires connect here Note polarity For normally open contact place jumper here For normally closed contact place jumper here Figure 6 2 Jumper Location Thermo Scientific Cryogenic Storage Vessel 6 3 Section 6 LN2 Level Monitor Operation Principles of The innovative Thermo LN2 Level Monitor utilises ultrasonic sound waves Op eration the level of liquid nitrogen inside a Locator or Locator Plus cryogenic storage vessel A microprocessor control provides continuous indication of the liquid nitrogen level inside the Locator or Locator Plus with accuracy to 2 Audible and visual alarms also provide an early warning of low level conditions Ultrasonic sound waves sense the level of liquid nitrogen inside the Locator or Locator Plus vessel By using ultrasonic sound waves to sense the level of liquid nitrogen no physical probe is required to sense the level of liquid nitrogen Conventional devices use probes which must be in physical contact with the liquid This physical contact increases liquid nitrogen consumpti
4. lightly dampened cloth containing mild soap solution Thermo Scientific Thermo Scientific Section 5 Replacement Parts Locator and Locator Plus Covers w o Monitor Covers and Rack Kits for units with lids made before 3 2010 Locator Jr Locator Jr Plus Locator 4 Locator 4 Plus Locator 6 Locator 6 Plus Locator 8 Locator 8 Plus Standard Covers CV509X51 CV509X51 CV509X51 CV509X51 CV509X50 321 CV509X50 321 CV509X23 31 1746 1746 1746 1746 651 651 7227 CV509X23 317227 CV509X23 317227 CV509X23 317227 Cryogenic Storage Vessel 5 1 Section 5 Replacement Parts Catalog Rack Description HR509X15A 317050 For Locator Jr 8141 8201 8202 with 4 2 inch boxes HR509X19A 315903 For Locator Jr 8141 8201 8202 with 2 4 inch boxes HR509X2A 70 317175 For Locator 4 Plus 8144 8207 8208 with 10 2 inch boxes HR509X31A 70 317192 For Locator 6 and 6 Plus 8147 8197 8198 8209 8210 with 10 2 inch boxes HR509X1A 317205 For Locator 8 8211 8212 8145 with 9 2 inch boxes 25 cell HR509X1A 70 317217 For Locator 8 Plus 8146 8213 8214 with 10 2 inch boxes 25 cell HR509X19A 70 315904 For Locator Jr Plus 8142 8237 8238 with 2 4 inch boxes 1 2 inch box HR509X20A 70 315906 For Locator 4 Plus 8144 8207 8208 with 5 4 inch boxes 1 2 inch box HR509X32A 70 315907 For Locator 6 and 6
5. HL uoneunsep go4J peddius eje sued 1ueujeoe doJ pue pied aBejsod oway 0 pauunja eq jsnuJ sued BuriuJojuoo uou je uondo s ouueu yy juaudinba Jo sjueuodujoo Aue Jo uJnjeJ JO I aoidde Joud af Jsnw jueumiedeq seorueg jeo1uyos eu poled jueJem jeuiBuo y puo eq Wed jueuoduioo y 0 Jo qjueuudinbe ay Jayya 0 Alueuem ey puojxe jou eys jueJem siy Jepun jueuudinbe JO sued sjuauoduloo Jo ed Jo jueuieoe dey QUeJem siy WO pepnjoxe eJe sjexseb pue say sep sway ejqepuedxa sureday Aue jo eoueuuojed oj sod uonoeJip pue uoneuriuJ9jep JUeJJIem JOJ p919ejuoo eq jsnuu jueuedeq sadas eoiuuoe eu ueuieoJ6e AjueJeM siy Aq peJeAoo ou 9Je uomneJqieo pue uonejejsu sJea 3314 40J peJeAoo si juBeju winnaeA zN7 pepnjoul Joqe esuedxe s ouuaeu 1e peoe daiJ Jo pauieda eq Wu diysuewyJom Jo sjenazew ui Buruuojuoo uou eq oj ue oud sued jueuoduuoo jee eut ayy Buung poued AjueJew Jeef SI ayy Buunp Jeuwo 1uenbesqns Aue o spuejxe uon 98 01d AjueJJe ay peJeAr ep si jueuudinboe noA eun wes ay Ajeyeuurxojdde je Pays out OD Ui jueJem y os euin Buiddius 104 smojje et Ayroej ano wo peddius si 3ueuudinboe noA opp ay WOA S499 OM SUEIS pollag AUeJIeM SUL unnoeA NU ALNVYIYVM LIONAOUd AUVANVLS SISILNAIOS YSHSI4 OWSSHL 8 1 Cryogenic Storage Vessel Thermo Scientific Section 8 Declaration of Conformity OE Declaration of Contormity Thermo Fisher Scientific Ashville LLC 401 Millcreek Road Marietta O
6. Plus 8147 8197 8198 8209 8210 with 5 4 inch boxes 1 2 inch box 5 2 Cryogenic Storage Vessel Thermo Scientific Section 6 Liquid Nitrogen Level Monitor Operation The liquid nitrogen LN2 level monitor provides a constant indication of the LN2 level and notifies you to low level conditions through audible and visual alarms It alerts you when your vessel needs filling The level monitor is shipped mounted directly to the appropriate Locator or Locator Plus cover It can be wired to a remote alarm system to alert you to problems when you are not in your laboratory This equipment was intended to be operated with an external power supply provided by the manufacturer It is designed for use with Thermo Scientific LN2 vessels only Caution Use this product for its intended usage only A I MN Warning Potential electrical hazards Only qualified persons should d 4 perform procedures associated with this symbol A Installation Note If the level monitor was purchased as part of a Locator or Locator Plus system skip to Step 3 If it is a retrofit for an existing Locator or Locator Plus begin with Step 1 A 1 Remove the level monitor and Locator or Locator Plus cover from box 2 Remove present cover from your Locator or Locator Plus and replace with your new level monitor and cover 3 Check the electrical specifications on the power supply for electrical data and plug in to appropriately grounded receptacle Thermo
7. Scientific Cryogenic Storage Vessel 6 1 Section 6 LN2 Level Monitor Operation Level Monitor 1 Disconnect the liquid nitrogen level monitor from the power supply Remote Alarm Disconnect power to your alarm system 2 Remove the three screws securing the liquid nitrogen level monitor s cover Pull the cover up and out of the way see Figure 6 1 Connection to Remote When connected to your remote alarm system the LN2 level monitor will Alarms alert you to a problem even when your laboratory is unoccupied The units alarm contacts are activated at the same time the audible alarm sounds see Microprocessor Functions 3 Remove the black plug from the side of the cover Insert the wires for the remote alarm through this hole 4 Connect the remote alarm wires as shown in Figures 6 1 2 and 3 5 Replace the LN2 level monitor s cover Reconnect power to the LN2 level monitor and to your remote alarm system Note The LN2 level monitor can be configured with either a normally open contact or a normally closed contact Which mode is currently operative depends on the placement of a single jumper on the circuit board For a normally open contact the jumper should be placed on J6 For a normally closed contact the jumper should be placed on J4 see Figure 6 2 Verify that the jumper is set appropriately for your remote alarm system A Alarm Contact The LN2 level monitors alarm contact is a Photomos type relay Specifications Contact
8. ad understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance A Caution All internal adjustments and maintenance must be performed by qualified service personnel A Caution It is recommended to have this vessel tested by the manufacturer or qualified cryovessel service technician every 7 10 years regardless of any problems or lack thereof you may have had in the past This will help insure your samples against sudden loss of liquid nitrogen due to vacuum failure A Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual Intended Use The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic temperatures These products are comprised of a vessel and a lid with or without a level monitor Unintended Use These products are not intended for use within the patient environment When this manual is translated into other languages the US English version is binding Thermo Scientific Preface Important operating and or maintenance instructions Read the accompanying text carefully Pote
9. and returned to the factory within 30 days to make warranty effective This information must include the serial number which is located on the label on the vessel Note The most prevalent cause of failure of liquid nitrogen storage vessels is mechanical The vessel necktube supports the full weight of the inner shell and all the liquid nitrogen it contains A side blow to the vessel causes the inner shell to swing in a pendulum like motion causing the necktube to be damaged Any storage vessel which has been in an accident has been dropped or lowered to hit on its side will tend to fail more rapidly than one that has not Caution Exercise caution when moving your Locator or Locator Plus IN vessel Locator and Locator Plus cryobiological storage systems are not transportation vessels Transport carts are designed for mobility within the labor lab to lab only Moving full vessels long distances over cracks in floor thresholds on inclined ramps or in elevators can cause premature vacuum failure Avoid using excessive force when moving vessel this can cause tipping resulting in damage and or spilling of liquid nitrogen A Note If samples must be transported under cryogenic conditions consider Arctic Express line of Dry Shippers available from Thermo Scientific Thermo Scientific Cryogenic Storage Vessel 3 1 A A N Filling Instructions Thermo Scientific Section 4 Operation Caution Never overfill your Locator or Locator Plu
10. cipate subtle changes in consumption and possibly react to problems before they arise You may opt to use a level monitor for this purpose see page 6 1 for level monitor operation Also if your samples are super critical one of a kind or irreplaceable consider allocating your samples to 2 separate vessels Remember the old adage about placing all your eggs into one basket Finally because no vacuum will last forever have your vessel tested by the manufacturer or a qualified cryovessel service technician every 7 10 years regardless of any problems or lack thereof you may have had in the past This will help insure your samples against sudden loss of nitrogen due to vacuum failure Do not use this product for anything other than its intended usage Use of the equipment in a manner not specified by the manufacturer may impair the protection provided by the equipment Cryogenic Storage Vessel 1 1 Section 1 Safety Information 1 2 Warnings Cryogenic Storage Vessel IN This unit is intended to be used by trained personnel in a well ventilated environment Liquid nitrogen is extremely cold it boils at 196 C Follow all necessary precautions and warnings relevant to applicable hazards such as frostbite contact burn asphyxiation etc in cryogenic area To avoid injury due to frostbite or ruptured vials use extreme care whenever handling liquid nitrogen liquid nitrogen storage or transfer vessels or any object
11. hio 45750 U S A Manufacturer Hereby declares under its sole responsibility that the following products s Product Description Cryogenic Storage Vessels Release ed S Cat N Model Release pos i Levels s BA at No No Levels s D CY509105CN CN509X7CN 316139 N A 2010 CY509108CN CN509X15CN 316305 N A 2010 CY509109CN CN509X16CN 315307 N A 2010 CY509110CN CN509X17CN 316309 2010 CY509111CN CN509X18CN 316375 N A 2010 CY509106CN CN509X19CN 316377 N A CY509107CN CN509X20CN 316390 N A 2010 Release level REL and date of manufacture shown on Serial Tag Conform s to the following European Union Directive s MDD 2007 47 EC This product conforms to the following Harmonized International and National Standards MDD EMC EN 61010 1 EN 61326 1 EN 61000 3 2 EN 61000 3 3 EN 60601 1 2 2007 CISPR 11 204 Ed 4 1 These products meet the applicable requirements of the MDD as transposed into national legislation of the member states where the product is placed on the market using Annex V combined with Annex VII of the Medical Device Directive 93 42 EEC as amended by 2007 47 EC Danish Medical Devices Certification EC certificate EDM 760 The authorized representative located within the European Community is Thermo Electron LED GmbH Robert Bosch Strasse 1 D 63505 Langenselbold Germany ac 28 August 2014 Eric Pickrell Regulat
12. ht position Do not use this Locator vessel for transport Cryogenic Storage Vessel 1 3 Section2 General Specifications EA Locator 4 Plus Locator 6 Plus Locator 8 Plus Locator JR Plus Height 37 5 VG a a 37 5 26 5 Fi including lid 95 2cm 39 5 100 3cm 39 5 100 3cm 95 2cm 39 5 100 3cm 67 3cm 28 9 73 4cm en Jem E zeen gcm 26 66cm EF gem 22 EE gcm 55 EE 22 urmem gcm LN2 Capacity Liquid Phase Static LN2 Evaporation Liquid Phase Capacity 2mL Vial actually 3600 4000 6000 6000 1800 2000 1600 2000 submerged in liquid nitrogen 1 The liquid nitrogen level monitor will add 1 inch to these heights The transportation cart will add 4 inches to these heights Using Nalgene System 100 cryoboxes with a 10 x 10 vial configuration Nalgene System 100 vial or equivalent are needed 3 Actual working time may vary due to current atmospheric conditions container history manufacturing tolerances and any individual patterns of use Environmental Operating temperature 17 C 27 C Conditions 20 to 80 relative humidity non condensing Installation Category II overvoltage in accordance with IEC 664 Pollution Degree 2 in accordance with IEC 664 Altitude limit 2 000 meters Storage 25 C to 65 C 10 to 85 relative humidity Locator and Locator Plus units are intended for indoor use only Thermo Scientific Cryogenic Storage Vessel 2 1 Section 2 General Specifica
13. ill take an accurate reading of the liquid nitrogen level Refer to the Specification Plate for the complete model number serial number and series number when requesting service replacement parts or in any correspondence concerning this unit All parts listed herein may be ordered from the Thermo Scientific dealer from whom you purchased this unit or can be obtained promptly from the factory When service or replacement parts are needed check first with your dealer If the dealer cannot process your request then contact Technical Services Prior to returning any materials contact our Technical Services Department for a Return Materials Authorization number RMA Material returned without an RMA number will be refused Cryogenic Storage Vessel 7 1 E LX60SAJ 06 91 NIOZX60GNI 8 191 OL LX60SN9 O6E9L NIOZXBOSNI gELgLE OL LX60SN9 SM1d 8 40LVIO1 EvE0S6 deg Hod 1u8A eulyo IVAY9Z 06 Ev LLLE SELXNL Alddns Du IVAOrZ 06 10128Uu00 SAY pue AN NI SN sepniout EELZLE ZXBOSNL SJ0 I9UU09 m A ddng Jamog EIXGOSAI ELXBOSAD ELXB0SAD ELXGOGAD ELXGOGAD ELXBOSAD ELXGOGAD EN 0102 E0 810489 6 191 pew spi jM NO X80SN2 206096 206096 016809861 80608561 90808561 1080856 suun 104 QuIyo SIN xoey pue Jano 010Z 0 ZEL9LE 8 0 9q apeu spi IXBOSNI 1090961 605056 2060561 8080861 060561 ULIM SHUN 104 SUN 198y pue 19407 eul EIS G0 91 G0
14. lacing unit on the vessel The green LED on the switch should be OFF 4 Cryogenic Storage Vessel 6 5 Section 7 LN2 Level Monitor Troubleshooting and Parts List Caution Preventative Maintenance Depending on environmental conditions and the number of lid openings the lid should be periodically inspected for ice and frost buildup Refer to chart below A ze e ee Level monitor alarm sounds intermittently Unit measuring liquid level incorrectly Level monitor measuring Full after Fog in top part neck area of removing samples and replacing lid cryogenic vessel Ordering Procedures Thermo Scientific Remove the cover w monitor from vessel and place in room for approx imately two hours or until no ice is visible on the transducer or tube NOTE Do not use an external heat source i e heat gun to accelerate Ice or frost buildup on the the thawing process as it can damage the cork Make sure lid is stand transducer ing upright on the cork to allow moisture to drain out of cover w moni tor Wipe any moisture away from the inside of the tube and face of the transducer with a clean soft cloth Place cover w monitor back on unit Remember to press Reset button The density of the fog that is formed after removing the lid and access ing samples is dependent on the humidity of the room where the cry ovessel is located The fog dissipates after about fifteen minutes and at this time the level monitor w
15. nal dip measurer part 180143 or Level Monitor 5 Fill Locator or Locator Plus as indicated see Caution in this section Allow for displacement of liquid nitrogen when racks and boxes are inserted 6 Insert and remove racks slowly Allow liquid nitrogen to run out of boxes and off racks Cryogenic Storage Vessel 4 1 Section 4 Operation 4 2 Measuring Liquid Nitrogen Quantity Storing Samples in Vapor Phase General Cleaning Instructions Cryogenic Storage Vessel me P Use a wooden yardstick or optional dip measurer part 4180143 to measure liquid nitrogen level NEVER use a hollow tube or plastic dipstick to measure the liquid nitrogen level Level will be indicated by the frostline which develops when dipstick is removed Level Monitor can be used for constant measurements See Section 6 Remove the 2 bottom most cryoboxes from each rack Measure the height of 2 cryoboxes stacked on top of each other Fill vessel with liquid nitrogen to the height obtained from Step 2 using a yardstick to measure liquid level in vessel Place racks into vessel without the 2 bottom most cryoboxes Samples are now above the liquid nitrogen and are stored in the vapor phase Important A level monitor should always be used when you store your samples in the vapor phase because of the already low levels of liquid nitrogen Refer to Section 5 for correct level monitor for your system A Wipe exterior surfaces with
16. ntial electrical hazards Only qualified persons should perform procedures associated with this E symbol IN Extreme temperature hazards Only qualified persons should perform procedures associated with this symbol k 2 Potential biological hazards Proper protective equipment and procedures must be used RX Marking of electrical and electronic equipment which applies to electrical and electronic equipment E falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermofisher com Y Always use the proper protective equipment clothing gloves goggles etc Y Always dissipate extreme cold or heat and wear protective clothing Y Always follow good hygiene practices Y Each individual is responsible for his or her own safety Thermo Scientific Cryogenic Storage Vessel iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you d
17. o please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the wa
18. on There is NO increase in liquid nitrogen consumption by using the Thermo level monitor Microprocessor The LED display is graduated in 8 segments and provides quick and Functions reliable visual indication of the level of liquid nitrogen inside the Locator or Locator Plus vessel from Full to Empty similar to a fuel gauge on an automobile A ee ee ag Load Voltage DC The position of J4 and J6 indicate whether the relay is normally open or normally closed Figure 6 3 Remote Alarm Connections Schematic 6 4 Cryogenic Storage Vessel Thermo Scientific Audi Thermo Scientific ble and Visual Alarms Safety Feature Level Monitor Operation Section 6 LN2 Level Monitor Operation LOW LEVEL indicator light illuminates continuously when liquid nitrogen level is below the approximate 2 level In this situation the level of liquid nitrogen is low and you are alerted to refill the vessel as soon as possible 2 An AUDIBLE and remote alarm activates continuously when liquid nitrogen is below the approximate 1 level inside the Locator or Locator Plus vessel additionally LOW LEVEL light will continue to flash In this situation the liquid nitrogen is dangerously low and you are alerted to refill the vessel immediately 3 The AUDIBLE and remote alarms activate continuously the 8 segment LED display and Low LEVEL lights flash to indicate an emergency event This alarm event occurs
19. ory Compliance Supervisor ThermoFisher SCIENTIFIC Rev 4 8 2 Cryogenic Storage Vessel Thermo Scientific Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 United States www thermofisher com
20. rranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is asal Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv Cryogenic Storage Vessel Thermo Scientific Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Table of Contents Safety Information ooooocccccccccccncncn nro 1 1 Please Read an EE EN 1 1 General Usage an an nee eg ada 1 1 WAND IIA AAA 1 2 CIT MEM CEDE 1 2 General Specifications 0 cece cece eee eee 2 1 Environmental Conditions 45 aussen e a use 2 1 Unpacking a een 3 1 Operation A ees date een 4 1 Measuring Liquid Nitrogen Quantity 000 00 e eee 4 General Cleaning Instructions 20 04 bes ERI a 4 2 Storing Samples in Vapor Phase 1 2 2 2 cae dee a 4 2 CAUtlbns ii Kees EA Hee VR Ee ee 4 2 Replacement Parts ee 5 1 Liquid Nitrogen Level Monitor Operation 6 1 E E id D Pp PS RE e ees 6 1 Remote ADI nee Poet e med D Ra tab rdc 6 1 Alarm Contact Specifications 22 E reir ku kl pie EE er EAS 6 2 Microprocessor Func
21. s vessel with liquid nitrogen The liquid nitrogen level in your tank with racks inserted should never be above 20 inches 50cm for Locator 4 and Locator 8 10 inches 25cm for Locator Jr 22 inches 55 8cm for Locator 4 6 and 8 Plus and 12 inches for Locator Jr Plus Filling the tank up to or above the bottom of the necktube may cause immediate or premature vacuum failure to occur A Caution When inserting or removing racks be careful not to come in contact with the necktube area of the vessel Remove or insert racks slowly in a vertical manner Scratches on the necktube area can cause premature vacuum failure A Caution Do not spill liquid nitrogen on vacuum port covered by a black plastic cap on side of vessel This can cause vacuum failure A To avoid damage to your Locator or Locator Plus cryogenic storage vessel which may result in premature vacuum loss it is important that the following procedure be used when adding liquid nitrogen to a warm vessel 1 Add only a small amount of liquid nitrogen 5 10 liters to a new or warm vessel 2 Allow this small amount of liquid nitrogen to sit in the covered vessel for a minimum of 2 hours This will limit stress caused by the sudden temperature change associated with adding liquid nitrogen to a warm vessel 3 Add an additional 15 liters of liquid nitrogen to the vessel 4 Allow vessel to sit for 48 hours and monitor liquid nitrogen consumption with a wooden yarsdstick optio
22. s which have come in contact with liquid nitrogen Leave no areas of skin exposed Wear appropriate PPE where necessary face shield cryogenic gloves cryogenic apron Do not tightly seal liquid nitrogen containers or prevent nitrogen gas from escaping Always handle ampules with tongs They may explode when removed from the vessel Use extreme care to prevent spilling and splashing liquid nitrogen during transfer and removal of storage contents and holders Immediately remove any clothing or safety attire on which liquid nitrogen has been spilled or splashed Get immediate medical attention for any frostbite injuries due to liquid nitrogen Thermo Scientific Thermo Scientific Cautions IN Section 1 Section title Only use the power supply provided by the unit Never overfill liquid nitrogen vessels Liquid nitrogen level should never be above the tops of the racks inside the vessel The excess weight may crack the neck and result in vacuum failure When inserting or removing racks be careful not to come in contact with the neck tube area of the vessel Remove or insert racks slowly in a vertical manner Scratches on the neck tube area can cause premature vacuum failure Do not tamper with or remove vacuum port covered by a black plastic cap on side of vessel this will void warranty Do not spill liquid nitrogen on vacuum port this can cause vacuum failure Always keep liquid nitrogen vessel in an uprig
23. tions Electrical Inputs 100 240VDC 08 04 amps single phase cover with monitors only O a Folic usta ne Een 47 63 Hz Output 422v 15VDC 10 0 2 amps 3 0 watts Declaration of CE models only We hereby declare under our sole responsibility that this Co nformity product conforms with the technical requirements of the following standards EMC EN 61000 3 2 Limits for Harmonic Current Emissions EN 61000 3 3 Limits for Voltage Fluctuations and Flicker EN 61326 1 Electrical Equipment for Measurement Control and Laboratory Use Part I General Requirements Safety EN 61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part I General Requirements per the provisions of the Electromagnetic Compatibility Directive 2004 108 EC Medical Device Directive 93 42 EEC as amended by 2007 47 EC C Copies of the Declaration of Conformity are available upon request 0543 2 2 Cryogenic Storage Vessel Thermo Scientific Section 3 Unpacking Before using your new Locator or Locator Plus vessel carefully inspect the vessel prior to use Check for signs of damage which may have occurred in shipment It is advisable to fill see filling instructions all new units with liquid nitrogen and watch liquid nitrogen loss rate for a few days If there are any problems call Customer Service as soon as possible The warranty registration card enclosed with the vessel must be completed
24. tions 1 664 a ak m Hee 6 4 Principles of Operation 2 cedere eR EET Eq mte LT ER 6 4 Level Monitor Operation ug Ah SOA ERG da 6 5 Safety Peri a e VERA SAX AQ e EC ERU UE T AS 6 5 Audible and Visual Alarms 100 wage eae e ee eee ee RS 6 5 LN2 Level Monitor Troubleshooting and Parts List 1 1 Ordering Procedures seed eme ic aet gine xe edat co dri akut 7 2 Cryogenic Storage Vessel V Please Read General Usage Thermo Scientific Section Safety Information This manual contains important operating and safety information The user must carefully read and understand the contents of this manual prior to the use of this equipment Your Thermo Scientific cryogenic vessel has been designed with function reliability and safety in mind It is the user s responsibility to install it in conformance with local electrical codes For safe operation please pay attention to the warnings and cautions throughout the manual All cryopreservation vessels regardless of who manufactures them use a vacuum to provide the super insulative properties needed to keep nitrogen in a liquid form They are very similar in fact to the Thermos vessels you may have used to store coffee soup or milk Remember how fragile they were Since no vacuum is perfect or will last forever we suggest that you monitor the consumption of liquid nitrogen used by your vessel on a regular basis i e every 1 2 days By monitoring you may be able to anti
25. when the Locator or Locator Plus vessel is empty of liquid nitrogen or the monitor has an error and is incapable of detecting LN2 levels The microprocessor must be reset each time the cover is removed from the Locator or Locator Plus Before removing the cover the RESET button must be pressed illuminating the RESET light While in this Reset Hold mode the monitor is not sensing After five minutes in the Reset Hold mode an audible and remote alarm will activate alerting you to deactivate the Reset Hold mode when the cover is replaced on the vessel Once the cover with the monitor has been placed on the vessel and plugged in wait about 15 minutes for the system to equilibrate before pressing the Reset button for an accurate measurement of the liquid nitrogen to be indicated This equilibration process will occur each time the cover is removed Each time a vessel is opened or moved the stabilized chamber temperature and or liquid level is disrupted The system requires a stable temperature and liquid level to determine an accurate liquid nitrogen level If you have just purchased a new Locator or Locator Plus along with your monitor allow at least 1 day for the liquid nitrogen to equilibrate the vessel before plugging in the unit This will avoid false alarms Note The Level Monitor may take up to 10 minutes to establish a reading after the unit is placed on the cryovessel or the cryovessel is moved Remember to press Reset after p
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