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User`s Manual

1. Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Copper Sulphate U 0 0 0 0 0 0 0 S 0 St 5 00000 0 Croesol S 0 0 00 S 0 U S US 0 0 8 M 8 0 Cyclohexane S 0 0 0 0 0 0 0Mt Sd Mt MU 0 0 0 0 0 Cyclohexanol Ss 0 U 00 00 5 MS S 0 0 85 0 8 0 Cyclohexanone 0 0 00 0 0 0 0M UU U U U 0 0 0 0 Cyclopentane 0 0 0 00 0 0 OM UU U U U 0 00 0 Decane 0 0 0 0 0 0 0 0 M Mt Mt Mt Sd O 0 0 0 O Dextran Sulphate MO 000 0 0 0 85 S S S S 0 S 0 0 Diacetone S 0 U 0 0 00 085 085 0 0 8 0 8 0 Diacetone Alcohol S 0 0 0 0 0 0 085 0 S S 0 0 0 0 0 0 o Dichlorobenzene 0 0 0 0 0 0 0 0M OU Mt M U 0000 0 p Dichlorobenzene 0 0 0 0 0 0 0 0M OU Mt M U 0000 0 Dichloroethane OU UU S O SU UU UO Os S os Dichlorophenol 0 0 0 00 00 0U UU U U 0 0 0 0 0 Diethylamine S 0 0 0 0 0 0 0 St U U St Sd 0 O 0 O 0 Diethyl Benzene 0 0 0 00 0 0 0 UM U U U 0000 0 Diethylene Glycol SS Ss ss Ss 0 US 85 S85 8 05 S S S Diethylene Glycol Ethyl Ether 0 0 0 00 00 0 SMS S M00 0 0 0 Diethyl Ether s 0 0 0 0 0 0 0U U O0 U 0 0 0 0 0 0 Diethyl Ketone S 0 U UM O OUUUMMO OS MS 0 Dimethylacetamide 0 0 0 00 00 0 85 USt S U 0000 0 Dimethylformamide S 0 0 0 0 0 0 085 U S S 0 0 SMS 0 Dimethylsulphoxide s 0 0 00 0 0 085 UO 85 0 8 S5 MS 0 Dioxane s UU 0M S 00M UM M O00 85 S SU Diphenyloxide S 0 0 0 0 0 0 0U 00 U 0 0 0 0 0 0 Dipropylene Glycol 0 0 0 00 0 0 0 8 Sd S S S 0
2. OID IO O oO Oo SR SR SRE 00000 0 0 0 0 0 U SSU U U U 0 0 0 0 0 00 0 S Carbazole U U U U U S 0 0 0 0 0 0 0 U Carbon Sulphide S S U M 0 0 0 0 0 SM S U S U S SM 0 0 0 0 0 0 0 0 0 U St MoO 0 0 0 0 0 0 St X Carbon Tetrachloride Cedarwood Essence Chlorine dry 000000 000000 St Sd St U 000000 0 Mt Sd St Mt Chlorine moist QO OO wo ojo julio O ju olojo jollon c Oo jo olojoljn jo O D D gt gt Oo gt o gt gt gt Aina gt n ele NM 2 gt Min EE S 3 2 3 52 2 gt 3 Ain gt gt jn lola ojojo gt pjojojojo o0jojojojojo jm jo Q Oo o jojo eue O S O S 3135 o0jo gt p jmiojoj gt ojo gt p gt jojo gt 3 olojojD jo jO D D SO S gt gt D c e SS o 3 Se S15 S ols 2 ho D ol5iso S9 g 98g to 4 B s S E L2 lt lt lt o SjalSlal elelo SIS CISISTE EI E olcS lojo jo o ol o lt Rl e s lt lt O ajojojao jo jo la 0 0 0 0 0 0 0 0 U St UUM 0 0 0 0 0 S Cinnamon Essence Citric Acid 1096 Copper Nitrate S S S 0 S 0 0 0 0 0 0 S S S S S S OMS SM SS S U 0000000 Thermo Electron Corporation CL31R IEC CL31 6 5 Chapter 6 Service and Maintenance
3. UU 00 0 Ss 0 0 0 Ss U M S 0 0 0 0 S 0 0 0 0 Potassium Hydroxide conc Potassium Nitrate 0 8 000000 S S DO S so S U 000 Ss Potassium Permanganate Propane Gas S 0 0 0 0 0 0 0 U St UM 0 0 0 0 0 U M Sd S 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sd Propionic Acid S 0 0 0 0 0 0 St S 0 0 00 00 0S Propyl Alcohol S 0 0 00 00 0 S Sd S ss 0 0 0 0 0 S 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 Propylene Glycol S S 0 0 0 S St 0 0 0M Propylene Oxide Pyridine US M 0 0 0 0 0 0 U 00000 0 0 0 S Sd Ss S Resorcinol Sat d Sol 6 8 IEC CL31 CL3TR Thermo Electron Corporation Chapter 6 Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Rubidium Bromide MO 0 00 0 0 OS 8 0 S 00000 0 Saccharose U 0 0 0 0 0 0 085 S S S S 0 0 0 0 0 Salycylic Acid Sat 0 0 0 0 0 00 0 S Sd S S S 0 0 0 0 0 Serum S 0 00 0 00 085 8 8 S 0 S S 0 0 Silver Acetate 00 00 0 0 0 0 S Sd S ss 0 0 0 0 0 Silver Nitrate U 0 0 0 0 00 085 8 S S S 0S 00 Sodium Acetate S 0 0 0 0 0 0 0 S Sd S ss 0 0 0 0 0 Sodium Bisulphate M O 3S 0 S 8 US 8 SS 9 O0 S 0 S 0 Sodium Borate MO 0 0 0 0 0 0 8 S Sd S 0 0 0 0 0 O Sodium Bromide U 0 0 0 0 0 0 085 50535 0 0 0 0 0 0 Sodium Carbonate
4. 2 M Ss SS oS oS S S S S D OSS oS Ss Sodium Chloride 1096 SS 58 350085 Ss Ss Ss SM S Sodium Chloride Sat d S S 0 0 S 00 SS 0 S SO SS SS S Sodium Hydroxide gt 1 UM S SU S 0S8 S US S SS SS SS Sodium Hydroxide 1096 UM U UU SOS S US SS SS SS SS Sodium Hydroxide conc U M U U U 0 0 0 M U S MO SS SM U Sodium Hypochlorite 5 MMS SUS Ss SsDS SS SMS SS S Sodium lodide MoO 0 0 0 0 0 0 S S O S 000000 Sodium Nitrate S 0 0 0 0 0 0 0 S 0 Sd S 00000 0 Sodium Sulfate S 0 0 0 0 0 0 0 Sd 0 Sd Sd 0 0 0 0 0 O Sodium Sulphide SS 35008085 35U S 80 S SM S Stearic Acid S 0 0 0 0 0 0 0 S Sd S S 850000 0 Sulphuric Acid 1096 MU S SU SS SS MSS S U S S S S Sulphuric Acid 2096 U 0 0 0 0 0 0 0 Sd Sd S Sd S 00 0 0 0 Sulphuric Acid 50 U U U U U SS U S S S S S US SM S Sulphuric Acid conc U U U U U S 0 U D U M D UMS S U S Sulphuric Anhydride dry or moist s 0 0 0 0 0 0 0 8 Sd S 5 5 0000 0 Tetrachloroethane M 0 0 00 00 0M 00M 0 0 0 0 0 0 Tetrachlorethylene 0 0 0 0 0 00 0U 08 U 0 00 0 0 0 Tannic Acid MoO 0 0 0 0 0 0 S 0 Sd S 0 0 0 0 0 O Tartaric Acid MoO 0 0 0 0 0 0 S Sd S S 0 0 0 0 O Tetrahydrofuran s 0 0 0 0 0 0 0U UU U U 0 S S500 Thionyl Chloride 0 0 0 0 0 00 O U UU UU 0 0 0 0 0 Toluene SUP SM S O U U UU U U S S SM M Trichlorethylene SU 0 0 0 Ss 0 UU UU U U US SM S Trichloroacetic Acid U 0 0 0 0 0 0 0Mt Mt Mt Mt S 0 0 0 0 0 1 2 4 Trichlorobenzen 0 0 0 00 00 O U UU UU 0000 0 Trichloroethane s U SOM SO SU UU UM OS sS 5 Triethylami
5. Rotor Removal dsd dd id 6 2 into Lab ion atea da 6 3 Chemical Compatibility Table for Rotors Tubes and Accessories rd 6 4 IEC CL31 CL31R ix User Manual Warning Symbols Sog B A Description Thermo Electron Corporation Chapter 1 Use and Function The user manual is part of the centrifuge and contains important information for your safety and for the best use of the equipment Always keep the manual close to the appliance and in a safe place so that it is always available Thermo strongly recommends that all users read this manual carefully The following symbols are provided to help the operator take advantage of the protection afforded by the equipment and to warn of potential danger On the main switch allows centrifuge to operate A On the main switch disconnects the centrifuge from the main power supply Only authorized personnel can touch the parts close to this symbol and in any case only after switching off the main power supply Coming in contact with high voltages could cause severe injuries A In this manual this symbol means that you will find important information for safety which if unobserved could result in damage to the appliance and or harm to the operator In this manual this symbol means that you will find important information about minimizing biological risk if unobserved the result could be harm to the operator The CL31 and CL31R centrifuges are designed for labo
6. Thermo Electron Corporation 6 19 Y 18 20 21 22 14 5 5a 4 4a LED Lights la Program 1 Indicator 2a Program 2 Indicator 3a Program 3 Indicator 4a Program 4 Indicator ba Program 5 Indicator 8a Acceleration Indicator 9a Deceleration Indicator 14 Lid Opening allowed Indicator 18 Speed Indicator 19 RCF Indicator 20 Time Indicator 21 Radius Input Indicator 22 Imbalance Indicator IEC CL31 CL31R 4 1 Chapter 4 Instructions for Use Display Screens Screen Description Upper screen 15 This screen displays the set speed rpm or RCF x g for the given program when the centrifuge is at rest depending on which parameter is selected During a run this screen displays actual speed or RCF Middle screen 16 This screen displays either the set run time or the centrifugal radius when the centrifuge is at rest depending on which parameter is selected During a run this display shows only time remaining timed runs or time elapsed during deceleration hold runs and pulse runs Lower screen 17 on the CL31 This screen displays either the acceleration or the deceleration profile selected for the given program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed Lower screen 17 on t
7. 0 0 0 0 Distilled Water SS SS S 0 8 SS SS SS SS US S S Ethanol 5096 SS S SM 8 SU S MS SS 8 S S S S Ethanol 9596 S S UUM S S U S U S S SSS SS 8 Ethyl Acetate MU U UM O UMUS U UO Ss S SU Ethyl Benzene 0 0 0 0 0 0 0 0M U St M U 0000 0 Ethyl Benzoate 0 0 0 0 0 0 0 0 Sd M S Sd U 0 0 0 0 0 Ethyl Butyrate 0 0 0 0 0 0 0 0 St U St St UO 0 00 0 Ethyl Chloride S 0 0 00 0 0 0 St U St St U 0 0 0 0 0 Ethylene Chloride s 0 0 0 0 0 0 O St U St St U 0 0 0 0 O Ethylene Glycol SS Ss ss S 0 US SS 8 ISOS S S S Ethylene Oxide 0 0 0 0 0 00 0M MMM 0000 0 Ethyl Ether S 0 U UO 00 0M UM M 0 OS MS 0 Ethyl Lactate 00 0 0 0 0 0 0 0 8 St 8 8 MO 00 0 Ethyl Malonate 0 0 0 0 0 0 0 00 SMS SMO 0 0 0 Fatty Acids S 0 0 0 0 0 0 0 Sd Sd Sd Sd S 00 0 0 0 Ferric Chloride U S 00M SS ss 0 S SOUS S S S Ferric Nitrate M 0000000 8 0 St M 00000 Ferric Sulphate S 0 0 0 0 0 0 0 0 S 0 0 0 0 0 0 Ficoll Paque MO 000 00 OS 858 5 0 5 00 Fluorine S 0 0 0 0 0 0 0 M Sd St M U 0 0 0 0 0 Fluorhydric Acid 10 UU MM U S 0 S S MS S SU SS SU O Fluorhydric Acid 5096 U U U U U S 0 0 S U S S MU S S UM Thermo Electron Corporation IEC CL31 CL31R 6 6 Chapter 6 Service and Maintenance Material BN CAB CN DL KY NO NY PA PC PE Chemical PPCO Formaldehyde 20 S Sd Formaldehyde 4096 S S Formaldehyde 50 S Sd Formic Acid 100 S M Freon TF Sd Sd Fuel Oil Sd Glucose Glutaraldehyde Glycerine Glycerol Heptane Hexane Hydrazine Hydrochloric Acid 5 Hydrochloric Acid 37
8. 0 0 0 0 085 US S Sd 00 0 0 0 Ammonium Acetate 0 0 000 00 085 535 0 0 85 Ss O Ammonium Carbonate SUS S 0 S 0 SS US 555550 Ammonium Chloride MO 0 OO 0 0 0 St O St SO 0 0 0 0 0 Ammonium Hydroxide 10 OS U 00 0 0 5 DU SDSS SS SS S Ammonium Hydroxide conc O U U 0000 SD US DO SS S S SU Ammonium Oxalate 0 0 0 0 0 0 0 0 Sd S S Sd S 0 0 00 O Ammonium Phosphate U 0000 00 085 MS S 0 0 0 0 0 0 Ammonium Sulphate S S 0 0 U 0 0 85 SS SOS 5 Ss O Ammonium Sulphide 0 0 0 0 0 8 00 5 U 0 8 0 0 85 0 0 0 n Amyl Acetate s 0 0 0 0 0 0 0 Sd U Sd Sd U O 0 0 O O Amyl Alcohol SM U 0S00SSSSsSsS00S sSSsSsM Amyl Chloride S 0 0 0 0 000 U U U U U 0 0 0 0 0 Aniline S 0 0 0 0 S 0 0 U 05 MO 0 S85 ss 0 Thermo Electron Corporation IEC CL31 CL31R 6 4 Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO 0 00 U Chemical S M U U 0 0 S S 0o S 8000000 0 8000000 U U 0 U 0 0 00 0 0 0 8 U U Aqua Regia Barium Chloride U 0 0 00 0 0 0 Barium Hydroxide Barium Sulphate Benzaldehyde S 0 0 0 0 0 0 S Sd M 0 0 0 0 0 0 St S 0 000 0 0 05 S 0 0 0 0 0 0 0 Sd Mt wlololmwlololololo AIO JOIN IO JOJO D O AIO JOIN O JOJO O jo v o lola O O O O jo AIO O lO O O O O jo SS 3 8 33 S 281815 je o jo o jo gt jojo 1 o o lo l gt o PIAS gt gt S gt gt gt 5318 a jm o o jo ID miojol gt jojojolo lo olo
9. 5 mL RIA 19 76 13 104 161 4100 3026 11175735 Set of 4 inserts 3mL 19 76 11 106 161 4100 3026 11175736 Set of 4 inserts 1 5 2 mL 13 52 10 42 159 4100 2988 11175738 Set of 4 inserts 15 mL vacu 4 16 16 124 163 4100 3063 11175749 Set of 4 inserts 7 mL vacu 12 48 12 5 106 160 4100 3007 11175740 Set of 4 inserts 5 mL vacu 12 48 12 5 75 160 4100 3007 11175753 Set of 2 cytobuckets 3 samples 12 samples 108 4100 2030 in open buckets only 3 5 IEC CL31 CL3TR Thermo Electron Corporation Chapter 3 Installation S41 Swing Out Rotor Cat No Description Capacity Max Min Max Radius Max Speed Max Tubeg Tube Ht mm rpm RCF xg mm mm 1210246 Swing out rotor 4 x 200 mL 161 4100 3026 1175611 Set of 4 bucket 200 mL 57 61 4100 3026 1175612 4 sealing lids 41193271 6 PP bottles with cap 200 mL 56 5 90 111 61 4100 3026 1174181 Set of 4 inserts 1x100 mL 45 80 121 61 4100 3026 1174501 Set of 4 inserts 1x50 mL flat bottom 35 56 123 61 4100 3026 1174528 Set of 4 inserts 1x50 mL conical 30 5 87 123 61 4100 3026 1174526 Set of 4 inserts 1 x 25 mL glass universal 29 87 124 61 4100 3026 1174527 Set of 4 inserts 1 x 25 mL plstic universal 25 86 125 61 4100 3026 1174502 Set of 4 inserts 3x 20 mL 22 80 120 6 4100 3026 1174537 Set of 4 inserts 3 x 15 mL conical 1 5 89 124 61 4100 3026 1174183 Set of 4 inserts 7x15mL 7 60 117
10. 6 IEC CL31 CL31R Thermo Electron Corporation Thermo Electron Corporation Chapter 4 Instructions for Use this reason the centrifuge is equipped with an imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides Also remember that the pins must be softly lubricated Inadaquate lubrication of the pins may cause incorrect swinging of the buckets resulting in imbalance phenomen See chapter 6 6 for details If the message persists despite your efforts to balance the load call your Thermo Service representative IEC CL31 CL31R 4 7 5 1 IEC 1010 2 020 IEC CL31 CL3TR Cautions Chapter 5 Hazards Precautions and Limitations of Use This centrifuge conforms to the IEC 1010 2 020 international standard which specifies particular safety requirements for laboratory centrifuges According to the standard warnings the operator must respect the following precautions e Mark a boundary area of 30 cm around the centrifuge e No person or hazardous material can stay within this boundary longer than necessary for operational reasons while the centrifuge is operating e An emergency switch for disconnection of the mains in case of malfunction has to be
11. Hydrochloric Acid 50 Hydrochloric Acid conc Hydrofluoric Acid 10 Hydrofluoric Acid 50 Hydrogen Peroxide 3 Hydrogen Peroxide 100 lodine Crystals Isobutyl Alcohol Isopropyl Alcohol Isopropylbenzene Kerosene Lactic Acid 20 Lactic Acid 100 Lead Acetate aq Lemon Essence Lime conc Magnesium Chloride Magnesium Hydroxide Magnesium Nitrate Magnesium Sulphate Manganese Salts Mercury Methanol 10096 Methyl Acetate Methyl Alcohol 10096 Methyl Butyl Ketone Methyl Ethyl Ketone gt m U U N N N mn N lt gt co co co c o co EL co C co e OC O 0 0 0 CO CO CO CO CO CO TC CO CO CO C C C C C C lt a a al a Z ou o mz L o E a z a a L oa co Ol co jco coo z co z c z aZ Cc El CO O o Ol Cl Ol o o an c eE ec El El El ol o o o al o nm ol El o aal Zoa nlololclololclolclo clolyv o jolclololo jo ololo jolclclolv v cl c ciololo o lolo ololo ololc lojolo olololcljololclolclo clolo o ololo lolo jo ololo jo clololv v cl lo clololo olo v ololo jolololojulo D O CO A OJ Of ZI GO AL Of A A co Df OJ HA DP OP OJ A A DP DJ OJ OJ DP DP olal A Gf HAL ololololo lv ololo ololv ojulo DLO A SDL OJ O A DOAJ A SD DOJ HLA ol olol A BD Daf AH olol AH A OQ Gf o
12. Liz Methyl Isobutyl Ketone St Methyl Isopropyl Ketone 0 Methylene Chloride X Mineral Oil 0 Nickel Chloride U Nickel Salts M 6 7 IEC CL31 CL31R Thermo Electron Corporation Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO Chemical o o in lo o O o in lo o Q co O nn O o o co O ja t O o ojajolaljnlolo O co c5 O 2 2 a nan z d la la S gt gt vja a 1 aan Iz IS I S 3 6 O QO co O o O O o ojn joln jo O o C 2 o 2 2 o o oln jo jo jolo ojmijol gt gt jojo 2 o 2 2 o o gt gt p gt Sl jo EEES sc CO S S ta S TiN EEEE elolololo c s alololo lo o e Oque Dlololololo 2S S 22224 ZIZ Z Z Z Z Z 0 0 0 S Sd S S Sd 0 0 0 0 0 0000058 0000000 S 0 00000 S0000 Octane Octyl Alcohol Oleic Acid S SS 5 5 SS S S S S Ss S S 0 0 0 0 MMS Ss 0 U M 0 0 0 0 0 S M Mt Mt S S S S 0 0 0 Sd Sd S Sd S 0 0 00 0 0 0 0 Mt 000 Ss 00 0 U S 0 Orange Essence Oxalic Acid S S S SM S S S S S S S S S S 0 0 000 S 0 0 000 S 0 0 000 s 0 0 00 0 0 0S S 0 0 00 0 0 0S Oxygenated Water 20 Oxygenated Water 50 Oxygenated Water 90 Ozone S 0 0 00 0 0 0 56 0 0 0 0 0 S 0 0 0 0 S 0 0 0 0 0 S 0 0 0 0 0 0 0 Paraffin 0 00 0 0 0 U U Pentane ojojn j
13. Pressing any button or opening the lid will bring the displays back to their normal at rest configuration display of set parameters Thermo Electron Corporation Chapter 4 Instructions for Use Preparing the First Before installation the rotor should be thoroughly inspected for Run of the Day evidence of corrosion or other damage and for cleanliness Chemical and stress corrosion of metallic parts will eventually lead to disruption of the rotor with potential severe damage to the centrifuge Particles stuck inside the pockets can cause breakage of tubes and lead to major imbalance and or loss of sample and contamination The central hole of the rotor and the drive spindle should also be clean and undamaged These parts should be wiped clean before each use The centrifuge also should be observed to verify proper appearance of screws lid hooks latches and for evidence of corrosion In case of problems contact your Thermo Service representative because any deviation from the above mentioned advice may have serious consequences for the safety of the appliance and of the operators Normal use of the centrifuge could require the manipulation of a biohazards Users and service personnel must have specific training for each substance they use according to the Laboratory Biosafety Manual from the World Health Organization Sam le Loadin The contents of each rotor pocket including sample tube cap and P 8 P P
14. Speed Max Angle Tube Y mm rpm RCF xg deg mm 41174928 Set of 20 adapters 1 x 500 800 uL 8 14600 23113 45 11174631 Set of 20 adapters 1 x 200 uL PCR 6 5 41174938 Set of 20 adapters 1 x 250 400 700 uL 6 Suitable for the AC 2 14 rotor DC 6 11 Microvolume Drum Rotor Cat No Description Capacity Max Radius Max Speed Max Angle Tube Y mm rpm RCF xg deg mm 11175743 Drum rotor 6 racks 72 14600 17156 90 11174561 1 rack 10x 1 5 2 mL 11 11174573 1 rack 20 x 500 800 uL 8 11174574 1 rack 20 x 700 uL 6 11174563 1 rack 21 x 600 uL 6 11174562 1 rack 32 x 250 400 pL 6 Total rotor load is 60 120 120 126 and 192 microtubes 3 7 IEC CL31 CL3TR Thermo Electron Corporation Chapter 4 Instructions for Use Controls and Indicators All the controls are located on the front panel The front panel is an intuitive interface no previous operational knowledge is necessary every parameter can be set by pressing the cursor key continuously from the minimum to the maximum and viceversa All parameters can be accessed and changed both during operation and while the machine is at rest 15 7 9 9a17 1311 12 B 1 5 Program Keys 6 Speed RCF Toggle 7 Time Radius Toggle 8 Acceleration rate set 9 Deceleration rate set 10 Stop Run 11 Pulse Run 12 Start Run 13 Temperature set R only Display Screens 15 Upper Screen speed RCF 16 Middle Screen time raduis 17 Lower Screen accel decel rates temp R mod
15. Thermo Electron Corporation For further information assistance or service United States of America Tel 1 888 213 17 90 Tel 1 866 984 37 66 Canada or Latin America Tel 1 866 213 17 90 Fax 1 866 984 37 66 China Shanghai Tel 86 21 68 65 45 88 Fax 86 21 6445 7907 86 21 6445 7830 India Mumbai Tel 91 22 5542 9494 Fax 91 22 5542 9495 Japan Tel 81 454 53 9220 Fax 81 454 53 9222 Other Asia Pacific Countries Tel 852 28 85 46 13 Austria Tel 43 1 80 14 00 France Tel 33 1 69 18 77 77 33 2 28 03 20 00 Belgium Tel 32 24 82 30 30 Germany Tel 49 61 84 90 69 40 Fax 49 61 03 40 81 012 Finland Tel 35 89 32 91 00 Thermo Electron Corporation Italy Tel 39 02 95 05 91 Netherlands Tel 31 7 65 71 44 40 Russia Cis Tel 7 09 52 25 11 15 Spain and Portugal Tel 34 9 32 23 31 54 Switzerland Tel 41 1 4 54 12 12 United Kingdom and Ireland Tel 44 87 06 09 92 03 Other Europe Middle East or Africa Tel 49 61 8490 6940 Tel 33 2 2803 2000 In Other Countries Contact your local THERMO products distributor or agent E mail Technical Service Representative for products at techsupport led asheville thermo com Visit our web site at www thermo com IEC CL31 CL3TR vii viii IEC CL31 CL31R Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents Uso aii FUNCOM rar ni 1 1 User Manli id 1 1 breui MT 1
16. adapter where used must be of the same weight as the one diametrically opposite centrifuge E Imbalance of the rotor may cause major damage to the rotor and Do not attempt to introduce liquids into rotor pockets or into tubes already in the pockets A Distribute the load correctly Loads should be inserted two by two samples of equal weight at 180 degrees from the rotation axis and at equal distance radius from the center of rotation see pictures When using fixed angle rotor weight deviation between the tubes must not exceed 5 grams Thermo Electron Corporation IEC CL31 CL31R 4 5 Chapter 4 Instructions for Use Correct ES Incorrect When using a swing out rotor the samples are placed in plastic inserts The load must be statically and dynamically balanced e Static balancing consists of balancing the weight of the 2 inserts which are in opposite locations in the rotor 8 to 10 grams e Dynamic balancing consists of placing the tubes in each insert symmetrically according to the axis of the trunnions Dynamic Balancing Correct E Incorrect If less than the maximum complement of samples is loaded the tubes must be placed in opposite pockets An odd number of tubes requires an additional blank water filled tube of identical total weight to balance Significant vibrations can damage the accessories the unit and the sam ples Most vibration is caused by incorrect loading of the tubes For 4
17. available and familiar to all the persons operating the centrifuge This switch has to be remote from the laboratory centrifuge preferably outside the room in which the centrifuge is located or adjacent to the exit from that room Moving or shifting the machine during centrifugation is dangerous e Using the centrifuge with rotating equipment showing evidence of corrosion and wear marks manipulating and or tampering with the electronic and mechanical parts are dangerous operations e Never try to bypass the lid lock safety while the rotor is spinning e Do not try to open the lid until the display returns to the stand by mode Only use a correctly grounded power source e Use only rotors and accessories designed for use in these centrifuges Thermo Electron Corporation Speed Control Operational Limitations Aerosol Risks Thermo Electron Corporation Chapter 5 Hazard Precautions and Limitations of Use Pay special attention to the following e Installation of the unit proper ventilation leveling of the centrifuge rigidity and stability of the support e Rotor installation verify that the rotor is locked in position before use e Cleaning of the accessories and of the rotor chamber is particularly necessary when corrosive products are present in the samples saline acids bases e Load balancing If any dangerous event occurs keep yourself far away from the instrument and switch off the main powe
18. corrosive liquids originating from overfilled uncapped tubes the liquid overflowing during centrifugation e Inserts adapters racks bottles whose exterior has been soiled by a chemical product or poorly rinsed after decontamination with bleach for example Note If the products are very corrosive simply rinsing is insufficient Residual traces dissolve little by little with the humidity present in the bottom of the rotor pocket Beware of the presence of solid particles beneath tubes inserts racks or adapters These particles are crushed by the centrifugal force and penetrate the protective anodized layer of buckets and rotors thus creating easy pathways for corrosion This term relates to the phenomenon of accelerated corrosion due to the effect of centrifugation when a corrosive chemical is in contact with the alloy From the time when the aluminium alloy has been attacked by chemicals stress corrosion begins to appear As it is on a microscopic scale it is even more dangerous than a macroscopic since it is invisible During centrifugation chemicals responsible for corrosion are also submitted to the very high g force which pushes them against the alloy This close contact facilitates the chemical reaction which occurs IEC CL31 CL31R 5 4 Chapter 5 Hazard Precautions and Limitations of Use Contamination Hazard 5 5 IEC CL31 CL3TR much faster than in a static situation Moreover centrifugal force
19. is very directional thus corrosion under stress creates with a very small amount of corrosive product straight microscopic fissures Each centrifugation run makes the chemical migrate further and further Fissures or cracks although it is microscopic are a cut in the metal breaking the cohesion of the material As one weak link in a chain allows the chain to break so the micro fissures break the chain of resistance of the accessory to centrifugal force As accessories are designed with high levels of safety rupture does not occur as soon as the first micro fissures are produced Depending upon the location of the fissure disruption may occur before it reaches the external surface of the accessory The fissure creates a weakness which makes the accessory less and less resistant to mechanical fatigue The corrosion by a small amount of corrosive product does not disrupt the accessory but makes it mechanically weaker and weaker until disruption due to both centrifugal effort and number of cycles Because stress corrosion is largely invisible it is essential that rotating equipment are scrutinized regularly paying particular attention to susceptible parts such as the bottom of pockets the outer edges and the base of the rotating equipment Thermo centrifuges are likely to be used in medical research and laboratory where hazardous substances are frequently present The user has the responsibility for carrying out appropriate deconta
20. ischen Richtlinien befindet Declaraci n de conformidad Nostros Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que el instrumen to bajo descrito est fabricado seg n las especificaciones establecidas por las siguientes normas europeas Declara ao de conformidade N s Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que o instrumento aqui apresentado est de acordo com as normas europ ias abaixo listadas Dichiarazione di conformit Noi Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France dichiariamo che lo strumento sotto descritto conforme alle seguenti Direttive europee e Machinery 98 37 CEE Low voltage 72 23 CEE 93 68 CEE e EMC 89 336 CEE 92 31 CEE 93 68 CEE e Safety standard EN 61010 1 EN 61010 2 020 Ch teau Gontier 28th June 2005 Thermo Electron Corporation IEC CL31 CL31R v vi IEC CL31 CL31R Guarantee Terms Thermo guarantees that this unit is free from defects in materials and workmanship when it leaves the factory and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR from the delivery This guarantee is invalid if the unit is incorrectly used poorly serviced or neglected mis used or accidentally damaged There is no explicit guarantee other than as stated above
21. la directiva RoHS explicado en www thermo com WEEERoHS France Conformit WEEE Ce produit doit tre conforme la directive euro p enne 2002 96EC des D chets d Equipements Electriques et Electroniques DEEE Il est merqu par le symbole suivant Thermo Electron s est associ avec une ou plusieurs compagnies de recyclage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Electron ces directives les recycleurs dans MU ocre pays et les informations sur les produits Thermo Electron qui peuvent aider le d tection des substances sujettes la directive RoHS sont disponibles sur www thermo com WEEERoHS iv IEC CL31 CL31R Thermo Electron Corporation Declaration of compliance Declaration of compliance We Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declare that the instrument defined here below complies with the following European directives Declaration de conformite Nous Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France d clarons que le mat riel d si gn ci apr s est conforme aux directives europ ennes suivantes Konformit tserkl rung Wir Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France erkl ren dass das hier beschriebene Ger t sich in bereinstimmung mit folgenden europ
22. mL 38 99 10000 11066 25 directly 1 x 94 85 80 mL 38 99 10000 11066 11174713 Set of 6 inserts 1x50 mL 29 93 10000 10397 11174714 Set of 6 inserts 1x50 mL con 30 93 10000 10397 Thermo Electron Corporation IEC CL31 CL31R 3 6 Chapter 3 Installation 11174715 Set of 6 inserts 1 x 30 38 mL 25 5 88 10000 9838 11174716 Set of 6 inserts 1x15ml con 17 93 10000 10397 11174717 Set of 6 inserts 2x10mL 16 83 10000 9279 11174718 Set of 6 inserts 4 x 1 5 2 mL 11 79 10000 8832 11174724 Set of 6 inserts 1 x 30 mL Corex 24 93 10000 10397 11174725 Set of 6 inserts 1x25 mL Corex 24 93 10000 10397 11174726 Set of 6 inserts 1x15 mL Corex Oak Ridge 18 92 10000 10286 AC 50 10A High Speed Rotor Cat No Description Capacity Max Radius Max Speed Max Angle Tube Y mm rpm RCF xg deg mm 11175754 Angle rotor 6 x 50 mL con round 29 5 x 118 112 10000 12520 40 11174606 Set of 4 adapters 1x15 mL conical 175x111 112 10000 12520 11177378 Set of 4 adapters 1x10mL 17x75 91 10000 10174 11174599 Set of 8 adapters 1x30 32 mL 26 105 10000 11738 Set of 6 conical to round adapter cushions supplied AC 2 14 Microvolume Rotors Cat No Description Capacity Max Radius Max Speed Max Angle Tube Y mm rpm RCF xg deg mm 11175741 Sealed angle rotor 24x 1 5 mL 1x39 84 14600 20016 45 11175642 Spare sealing lid FIBERLite Micro Cat No Description Capacity Max Radius Max
23. the instructions below before sending an instrument or Mr Mes names ere atos tert a ne Establishment cinc Department scooter tede pee aat titt t en tede dece dated WOES nr er eor nee tat ER Aer ELLE Post ZID CONE ie M State Country A E Ei Serial N iuge net tee RD TR Serial N OO D Serial N Atcessory Descriptions sarsa e dei pan Serial N Accessor DESC sen tir MA NR Serial N Nature of contamination Decontamination certified by MEME a e a Institution BAR RS Signature When an instrument or parts of it requires servicing by Thermo personnel the following procedure must be accomplished to ensure personnel safety e Clean the instrument and proceed to its decontamination from any kind of dangerous products e Compile this Decontamination Certificate with all the information required 1 1 IEC CL31 CL31R Thermo Electron Corporation Thermo Electron Corporation Certificate of Decontamination and Cleaning e Attach this Certificate to the instrument or part before sending it to Thermo Service or other authorized technical service Thermo personnel will not accept to work on instruments deprived of this Decontamination Certificate If an instrument is received at our Service facilities and in our opinion is a radioactive or biological hazard the item will be refused and resent to the Customer Disposition costs will be borne by the sender
24. with the centrifuge disconnected from the power outlet No daily cleaning is required except in the case of accidental tube breakage or when there is a large amount of spillage in the bowl Clean the bowl and the accessories with a cotton wool pad dipped in 70 alcohol Never use metallic brushes to clean accessories e After cleaning the accessories rinse them in running water preferably distilled water Dry the rotor with a soft absorbent non woven cloth or tissue Drying may be finished off with a warm air jet e g a hair dryer e Make sure that the pockets are well dried e Ideally spray with Anti Corrosion Spray Cat N 11175399 Store unused buckets upside down on a non metallic grid to allow free passage of air Alcohol 7096 ethanol or isopropanol applied for 10 minutes is ideal for bacteria and viruses Autoclave for 20 minutes at 120 C to destroy micro organisms Polypropylene components are autoclaveable Rotor lids must be disassembled from rotor bodies O rings autoclaved separately should be replaced when deformed Any part which has been subjected to temperatures above 130 C must be discarded Hypochlorite bleach used at 0 196 concentration with 10 minutes immersion is effective against bacteria spores and viruses but as an oxidizing agent is corrosive to metal alloys and must be thoroughly Thermo Electron Corporation Radioactive Decontamination Power Supply Circuit Breake
25. 1 D COR A nan tete 1 1 Refrigeration and Heating Stemi pais 1 2 Drive Syste RET Om 1 3 Safety Interlock System Rd a TE 1 3 Tmbalance Detection Syst m sessenta hausses 1 3 Relative Centrifugal Force inscrita 1 4 Specifications 2 1 Dimensions and ric c 2 1 Electrical Specifications ae abest ei itat sunitas DUM Rua HOMER 2 1 Performance acest resessie i ees NEST E EEEE EE EEEE EOE ENES 2 1 tala rca iai 3 1 Environmental Condo ia 3 1 A 3 1 Porton A iii 3 2 A M 3 2 Lid Opening and Rotor Checking 2 4 3 2 Manual Lid Unl cking Proced re il 3 3 Performance and Accessories D mean 3 5 Instructions for USO 4 1 Controls and Indicators ia ttene 4 1 Display Screens pp 4 2 Control Panel FAISONS airada 4 2 Control Panaderia 4 3 Description of Certain Events with session 4 4 Preparing the First Run of the Day iicet iii 4 5 Sample P 4 5 Hazards Precautions and Limitations of Use 5 1 TEG TAD 250 T li 5 1 A A 5 1 Speed Control utili Dal aaeeea iaeaea eani 5 2 Operational RMS Ad eneas SRS O 5 2 Aerosol REKS aeaa r A q 5 2 Metal AS a ie 5 3 Thermo Electron Corporation Thermo Electron Corporation Chapter 6 Contents Service and Maintenance eese ner etn rentas 6 1 Periode Clin ee dd 6 1 PRENONS A ER LUPO 6 1 Radioactive Decontamination eese eene 6 2 Power Supply Circuit Breaker ns rnit ticae icu 6 2
26. 1 118xR Speed rp m 946 RCF RCF R V x 946 R Radius mm RCF N x x 1 118 M RCF MR 1 118 N Transformations Key sequence To determine actual g achieved at a different speed RCF2 RCF1 MI N2 NI x x RCFI N1 To determine actual speed required to achieve a different g at the same radius N2 N1 RCF2 RCF2 RCF1 V x N1 RCF To determine actual speed required to achieve the same g at a different radius N2 N1 Ri R1 R22vVxN1 R2 Thermo Electron Corporation IEC CL31 CL31R 1 5 Chapter 2 Specifications Dimensions and Weight CL31 CL31R Dimensions HxWxD 375 x 400 x 500 mm 380x 575 x 620 mm Packed HxWxD 600 x 580 x 640 mm 630 x 730 x 750 mm Weight uncrated crated 40 kg 52 kg 72 kg 85 kg Electrical Specifications CL31 CL31R Operating Voltage 1096 230 V 120 V 100 V 230 V 120 V 100 V 100 V 50 60 Hz 60 Hz 50 60 Hz 50 60 Hz 6 0Hz 50 Hz 60 Hz Operating current steady 2 8 Arms 4 3 Arms 4 9 Arms 29 Arms 6 8Arms 7 Arms 8 1 Arms acceleration 5 5 Arms 9 5 Arms 10 5 Arms 9 Arms 13 3 Arms 13 Arms 15 Arms Power max steady 350W 600 W acceleration 1000 W 1300 W Heat dissipation 1195 BTU h 2050 BTU h Performance CL31 CL31R Max power 500 W 800 W Average power 350 W 550 W Refrigeration 235 W CL31 CL31R Max speed Swing out 4100 rpm Angle 14600 rpm Max RCF Swing out 3176 xg Angle 23113 xg Max capacity Swing out 4 x 280 ml An
27. 6 4100 3026 1174509 Set of 4 inserts 3 x 15 mL Vac 1 5 79 126 6 4100 3026 1174503 Set of 4 inserts 5 x 10 mL Vac 6 58 121 61 4100 3026 1174534 Set of 4 inserts 5 x 75 710 5 10 mL 17 42 118 161 4100 3026 1174533 Set of 4 inserts 5 x Z5 5 mL 17 42 61 4100 3026 1210764 Set of 4 inserts 7 x 5 7 mL Vac 13 58 121 161 4100 3026 w Hemogard 1174505 Set of 4 inserts 9 x 5 6 mL Vac 13 5 60 118 61 4100 3026 1175577 Set of 4 inserts 12x 5 mL open 12 5 72 115 161 4100 3026 1174506 Set of 4 inserts 12x 3 5 mL round bottom 12 42 116 161 4100 3026 1174508 Set of 4 inserts 6x1 5 2mL 11 45 120 61 4100 3026 1175613 Set of 2 3 cyto samples 18 4100 2218 cytobuckets per bucket T20 High Throughput Rotor Cat No Description Capacity Max Min Max Radius Max Speed Max Angle Tubeg Tube Ht mm rpm RCF xg deg mm mm 11175750 Swing out rotor 6 x microplates 115 4100 2160 90 with carriers or 2 x blocks 11175631 2 sealed carriers 3 plates 1 block each 11174207 Rubber cushion for flexible microtiter plates 11210762 2 adapters for 7 25 and T 75 Falcon type Cell Culture Flasks AC 15 4 High Throughput Blood Tube Rotor Cat No Description Capacity Max Min Max Radius Max Speed Max Angle Tubeg Tube Ht mm rpm RCF xg deg mm mm 11175755 Angle rotor 30 x 15 mL 17 5 100 120 135 4100 2537 37 AC 100 10A High Capacity Angle Rotor Cat No Description Capacity Max Radius Max Speed Max Angle Tube mm rpm RCF xg deg mm 11210437 Angle rotor 6 x 100
28. HS Conformita WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici WEEE E marcato col seguente simbolo Thermo Electron ha stipulato contratti con una o diverse societ di riciclaggio smaltimento in ognuno degli Stati Membri Europei Questo prodotto verr smaltito o riciclato tramite queste medesime Ulteriori informazioni sulla conformit di Thermo Electron con queste Direttive l elenco delle ditte di riciclaggio nel Vostro paese e informa zioni sui prodotti Thermo che possono essere utili alla rilevazione di sostanze soggette alla Direttiva RoHS sono disponibili sul sito www thermo com WEEERoHS WEEE Conformidad Este producto requiere cumplir con la Directiva 2002 96EC WEEE residuos de quipos electricos y electronicos Este producto esta marcado con el simbolo WEEE como se describe En cada Estado Miembro de la Union Europea EU Thermo Electron ha contratado con diversas companias para disponer y o re ciclar los resi duos de equipos electricos y electronicos Existe information adictional acerca de la conformidad de Thermo Electron con esta Directiva incluyendo los nombres de las diversas companias autorizadas para dis poner recyclar nuestros productos Adicionalmente suministramos IEC CL31 CL31R iii WEEE Compliance informacion acerca de los productos de Thermo Electron que puedes asistir en la deteccion de substancian mensionades en
29. IEC CL31 CL31R Multispeed User s Manual Analyze Detect Measure Control ELECTRON CORPORATION IEC CL31 CL31R Multispeed User s Manual IEC CL31 CL31R Multispeed Thermo ELECTRON CORPORATION Carefully read this manual before operating your instrument Information contained in this document is the property of Thermo Electron Corporation it may not be duplicated or distribuited without the owners authorization The validity of the guarantee is subject to the observation of the instructions and precautions described in this document Preface MANUAL NUMBER REV ECR ECN DATE DESCRIPTION BY a 11290087 02 06 Initial release Thermo Electron Corporation IEC CL31 CL31R i IEC IEC CL31 CL31R Packing List EJ 11210913 Centrifuge IEC CL31 230V 50 60 Hz 11210914 Centrifuge IEC CL31 120V 60 Hz EJ 11210915 Centrifuge IEC CL31 100V 50 60 Hz L 11210917 Centrifuge IEC CL31R 230V 50 60 Hz L 11210918 Centrifuge IEC CL31R 120V 60 Hz 11210919 Centrifuge IEC CL31R 100V 50 Hz L 11210920 Centrifuge IEC CL31R 100V 60 Hz Item number Quantity Check Centrifuge 1 E 11290087 User manual Manual lid opening instructions E 64227001 Mains cord E Toolbox 11202979 Rotor removal tool 25535010 Emergency unlocking tool 2 E 26387032 Spare fuses CL31 only 1 For missin
30. Instructions for decontamination and cleaning are explained in the User Manual Additional certificates are available from your local technical or Customer Service representative In the event these certificates are not available a written statement certifying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable For your convenience use a copy of this page IEC CL31 CL3TR 1 2 11290087 a
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32. al safety standards related to protection against indirect contact the supply of power to the instrument must be via a power socket fitted with a protection device ensuring automatic cut off in the case of an insulation fault A supply fitted with a circuit breaker of the correct rating complies with this requirement Ensure that the centrifuge has been switched ON Pull the handle on the centrifuge right side towards the front of the unit the lid is automatically unlocked and opens In the case of a mains power outage opening of the lid is prevented by the lid lock safety device It is recommended to wait for the mains power to be switched back on so that this safety device enables the lid to be unlocked refer to 3 6 for manual lid opening Carefully clean the inside of the centrifugation chamber removing any packing residues In fact due to air turbulence solid particles accidentally left in the centrifugation chamber create excessive wear of the chamber itself and of the outer rotating equipment surface Carefully lower the rotor onto the drive shaft Press down on the rotor until a click is heard Try to lift the rotor When correctly placed it will not move being automatically locked onto the drive 1 2 we XQ uM IEC CL31 CL3TR 3 2 Chapter 3 Installation It is not necessary to orientate the rotor relative to the drive shaft in order to achieve locking The AUTO LOCK rotor mounting system allows rotors to
33. arrows Set temperature R only To increase decrease the temperature set values press on the corresponding up down arrows To pre program the centrifuge There are five available memory locations for pre programmed protocols Each corresponds to one of the five memory buttons 1 5 To enter save a program into one of the locations press the memory button corresponding to the desired program position The corresponding LED 1a 5a will be illuminated While the LED is illu minated enter the desired parameter values These values are automatically saved in memory To lock a program To lock a program press and hold the corresponding button for several seconds until the correspon ding LED begins to flash Release the button and the program is locked Parameters of the program cannot be changed To unlock a program To unlock a program when a program is locked the associated LED flashes press and hold the corresponding button for several seconds until the associated LED stops flashing Release the but ton and the program is unlocked Control Panel Indicators Indicator o Q Description When the lid interlock indicator LED 14 is illuminated the lid interlock is not active The lid can be opened The indicator will only be illuminated when the rotor is at rest and it is safe to open the lid When the lid interlock indicator LED is not illuminated the lid cannot be opened The rotor is still in motio
34. basic safety features e The run cannot be started if the lid is not correctly closed e The centrifuge lid cannot be opened if the rotor is running Current safety regulations allow the centrifuge lid opening when rotor speed is below a certain defined level very few rpm This conditions is therefore not to be considered a fault or riscky for the operator A The centrifuge will not operate until the lid is closed and latched in place The lid remains latched until the rotor stops spinning The lid access lamp indicates when the rotor has stopped consequently the handle on the right hand side of the centrifuge can be used to open the lid If a power failure occurs access to the samples in the centrifuge is possible via a manual lid opening procedure see appropriate paragraph For this operation a special tool supplied with the centrifuge is required Bypass the interlock system only under emergency conditions as the rotor could still be rotating A The CL31 centrifuge is equipped with a load imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be IEC CL31 CL31R 1 3 Chapter 1 Use and Function 1 4 Relative Centrifugal Force Configuration formule IEC CL31 CL3TR decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diamet
35. be placed in any orientation Manual Lid Unloc king In the event of mains non availability or power failure opening of the Procedure lid is prevented by the lid locking safety device It is recommended that the user waits for the mains to be switched back on so that this safety device enables the lid to be unlocked Manual lid unlocking must only be done by someone informed of the possible danger and of the necessary precautions Rotating parts are a risk as they could come in contact with the user or be ejected There is particularly high risk of injury if e The user attempts to manually stop the rotor Any object falls inside the centrifugation chamber while the rotor is running Manual lid unlocking may be necessary under a very limited number of conditions such as the urgent recovery of critical samples that could be damaged if left in the centrifuge rotor In this case the lid can be opened by using the special tool supplied with the centrifuge ur Always set the power switch to the OFF position before performing this manual procedure even in the case of a mains power outage Should the emergency occur due to power failure during centrifugation wait at least 10 minutes for the end of the rotor rotation In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually Upon opening lift the lid by hand and observe the rotor be careful in this operation if it is still rotati
36. ends the use of its sealed buckets which are certified for bio containment through worldwide recognized testing procedures performed in recognized external laboratories Aerosols are produced naturally when using uncapped tubes or bottles They are also produced when a container deforms or breaks provoking a small or large sample leak We draw your attention to the use of tubes in angle rotors Even if the liquid level does not touch the cap when in a static state it could do so during centrifugation and leak You should therefore follow the instructions of the tube supplier concerning the fill level and sealing of the tube Once a biological risk is known or suspected samples should be placed in a sealed container Should such containers not be available sealed accessories should be used such as angle rotors with sealed lids P l l l l gt c When aerosols are created in a centrifuge bowl they persist in the air for a period of 3 5 minutes after the rotor stops moving Indeed the action of opening the lid itself causes the dispersion of aerosols into the environment When an elevated risk of aerosols or of breakage is perceived the accessories should be handled using gloves and opened in an environment ensuring the protection of the operator safety cabinet glove chamber wearing a mask even if they are apparently sealed A Thermo rotors made of aluminium alloy are designed to operate at their rated RCF
37. for many years With careful use they will resist corrosion lowing the possibility of excessive imbalance disruption and subsequent damage to the instrument The primary conditions for the initiation of corrosion exist in every laboratory during daily use of the centrifuge For this reason it is essential that due care and attention be paid to inspection and cleaning Thermo Electron Corporation Chemical Corrosion Stress Corrosion Thermo Electron Corporation Chapter 5 Hazard Precautions and Limitations of Use This corrosion is characterized by chemical reactions due to the existence of any electrolyte liquid on the surface of the rotating equipment If these substances are allowed to remain on the surface the corrosion will almost certainly occurs This produces first a discoloration of the anodization the pitting of the metal Acidic and alkaline solutions sustaining their pH level will create problems of corrosion in aluminium equipment Chlorides present in salts or even in skin contact with the rotating equipment are among the most aggressive and harmful substances commonly found in the laboratory The chemical products which are the origin of this corrosion do not necessarily originate from broken tubes for example they could come from e chemical vapors present in the laboratory which are dissolved in the residual humidity in condensed water refrigerated centrifuges present at the base of the rotor pockets
38. g parts call your nearest Thermo representative Thermo Electron Corporation Great Britain Deutschland Lx Italia Lx rri o Ss e 9 Thermo Electron Corporation WEEE Compliance WEEE Compliance This products is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96EC It is marked with the following symbol Thermo Electron has contracted with one or more recycling disposal companies in each EU Member State and this products should be disposed of or recycling through them Further information on Thermo Electron s compliance with these Directives the recyclers in your country and information on Thermo Electron products which may assist the detec tion of substances subject to the RoHS Directive are available at www thermo com WEEERoHS WEEE Konformittat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96ECerfiillen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Electron hat Vereinbarungen getroffen mit Verwertungs Entsorgungsanlagen in allen EU Mitgliederstaaten und dieses Produkt muss durch diese Firmen widerverwetet oder entsorgt werden Mehr Informationen ber die Einhaltung dieser Anweisungen durch Thermo Electron die Verwerter und Hinweise die Ihnen n tzlich sein k nnen die Thermo Electron Produkte zu identizfizieren die unter diese ROHS Anweisung fallen finden Sie unter www thermo com WEEERo
39. gle 6 x 100 ml Microprocessor controlled Display High visibility digital display Memory size 5 programs direct access Program protection Recall key lock Speed Range 500 to 14600 rpm Step 10 100 rpm Accuracy 20 rpm Timer Range 30 sec to 99 min hold position Step 30 sec to 1 min 2 1 IEC CL31 CL3TR Thermo Electron Corporation Chapter 2 Specifications CL31 CL31R Acceleration rates 5 Braking rates 5 Temperature CL31R Range 9 C to 40 C Step 1 6 Accuracy 1 5 C Typical performance 4 C at 4 000 rpm 4 x 280 ml swing out 1 C at 14 000 rpm 24 x 1 5 ml angle Maximum density 1 2 g cm3 Maximum energy 14 400 J Thermo Electron Corporation IEC CL31 CL31R 2 2 3 1 Environmental Conditions Unpacking IEC CL31 CL3TR Chapter 3 Installation General conditions accepted for centrifuge transport and storage are Ambient temperature 20 C to 50 C e Relative humidity up to 90 General conditions accepted for operating the centrifuge safely are e Indoor use Temperature 5 C to 40 C e Maximum relative humidity of 85 e Maximum altitude 2000 m e Installation category II e Polution degree 2 Due to the weight of the machine all lifting and transporting must be carried out using proper handling equipment i e fork lift trolley that complies with current regulations and by people having undergone the necessary traini
40. he R units This screen displays either the acceleration or the deceleration profile selected for the given program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed In addition it shows the temperature in the bowl of the centrifuge When the centrifuge is at rest the set bowl temperature is displayed C During a run the actual bowl temperature is displayed C Control Panel Functions Function Start cycle Keystroke sequence and description Press the start button 12 Stop cycle immediate Press the stop button 10 Pulse Press the pulse button 11 and hold The centrifuge will accelerate the rotor to the speed setting of the active program When the button is released the rotor will be decelerated to rest Set speed When the LED next to RPM 18 is illuminated the upper display shows speed values To increase decrease the speed values press on the corresponding up down arrows Set RCF When the LED next to G 19 is illuminated the upper display shows RCF values To increase decrease the RCF values press on the corresponding up down arrows When using RCF as a control display value make sure the correct centrifugal radius is entered into the control system see below If an inc
41. ing to a proper value During the run the upper display will show either actual speed or actual maximum RCF values whichever is currently selected The middle display will show either remaining time timed run or elapsed time hold and pulse runs During a run the middle display will always show time The user cannot change the display to show the given radius value during a run Most set parameters can be changed during a run when the selected program is not locked Set speed can be changed by selecting speed as the control parameter for the upper display then by pushing the up or down arrow Set run time can be changed by pressing the up or down arrow corresponding to the middle display Set deceleration profile can be changed by first selecting the deceleration profile and then using the up down arrows When the stop button is pressed or when the run timer reaches zero the centrifuge will begin to decelerate the rotor At this point the run timer will stop and the middle display will begin to show elapsed braking time The upper display will show either actual speed or actual maximum RCF values whichever is currently selected The lower display shows the given deceleration profile and the LED corresponding to the acceleration button is illuminated At the end of a run after the rotor has been decelerated to rest the centrifuge will produce an audible beep and the middle display will show the word End
42. jojojolojolo jo ololololololololo gt jo jo H jolo jolo o ololololololololo o loljolD jo jojololo gt DjoljojD o lolololo co c5 o c 2 2 2 2 c a g re 2 S 9 wm Si 8 N qajo Sim SIElo 2 o E 12 2 28 0 D O mm O 1 0 S BIBIBSISISIBS o o o o o lge 9 5 m oo co Joo a Ia jojo ja 000000000 S 0 0 0 0 0 0 0 0 Butane S S S 0 0 0 0 0 08 00 U 0 MS S MOS s 0 0 0 0 00 00 085 00 0 0 0 0 0 U S S 0 0 00 0 0 0 6 n Butanol U U S S n Butyl Acetate Butylene U U U 0 0 0 0 0 U S 0 0 0 0 0 U U 00000000 U Butyl Chloride Butyric Acid S 0 0 0 0 0 0 0 U Mt CIO O 10 JO JO JO O 1M 1M O ololjnlo jo jo jojo ju jun o a lo la O O joljolo a en en lo lo lau lo ju jm lo O O O O O O n 5D O nj lo jo jo jp jo 1M jun lo ona lo lo en jo ln ja jo lo a en en lo lo lo l lo lo s olojolo jo ln ln O gt S jo NIM en lo jo jo 1M jo lo O O O JO JO JO 1M 1M O Oojojojojojojojolojo lo O 0 O JO JO JO O JO MIO ce Ojo OS 1S S O e 2 10 0 IO MIO JO O 2 O O OO ololjnlo jo jo s joljnljolo S 0 0 0 0 0 0 0O N D O gt gt gt gt gt 2 gt gt D gt gt gt D 2 E 2 o o D D O olelel D C Oo lS OIS S rlels elolo j2 lolS ols 2 E lS 2 S o 38 2Q olzl lt lols Sl lslel s TIDO e jan lE CS C5 E co elelelelelele alala lalala 2 E 5 E IE oDio olo o oO 9 29 99
43. layed on the front panel of the centrifuge Under certain conditions high speed long duration runs the sample temperature can be higher than the bowl temperature on the display In this case the user may compensate manually after empirical determination of the temperature differential The lid should remain closed when the refrigeration system is in use to avoid condensation 3 l 0 Moe REFER TO INSTRUCTION MANUAL The centrifuge has a rubber hose that allows condensation to be drained from the bowl The drain hose exits at the back of the machine Near the drain hose you can find the following label To remove condensation please follow this procedure e Switch off the centrifuge and disconnect the power e Puta small basin under the hose e Unplug the hose Thermo Electron Corporation Drive System Safety Interlock System Imbalance Detection Thermo Electron Corporation System Chapter 1 Use and Function e Drain the centrifuge bowl e Insert the plug back into the hose Attention Do not remove the plug from the drain hose during centrifugation A three phase brushless motor drives the rotating equipment The rotor is contained in a sealed armor plated centrifugation chamber The brushless motor requires no maintenance A control system ensures the correct drive speed which is continuously monitored The CL31 and CL31R centrifuges are equipped with an interlock system that assures two
44. lrin KY Kynar M Moderate resistance NO Noryl NY Nylon U Unsatisfactory PA Polyallomer PPCO PC Polycarbonate X Explosion risk PE Polyethylene PP Polypropylene O No information PPCO Polypropylene Copolymer PS Polysulfone d Less resistance if T gt 50 C SS Stainless steel TF Teflon t Unsatisfactory if T gt 50 C TZ Tefzel T Titanium VA Viton A Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Acetaldehyde S U U U O 0 0 OM UMMO SS Ms U Acetamide 0 0 0 0 0 0 0 08 US S 350000 0 Acetic Acid 5 SMS SM SS SS SS DS SS Ss S M Acetic Acid 20 S 0 0 0 0 0 0 085 MS 8 80000 0 Acetic Acid 6096 S U U U U Ss SMS UMD S SS SSU Acetic Acid 80 S 0 0 0 0 0 0 0 S U S amp S MO 0 0 0 0 Acetic Acid Glacial S U U U U S 0 0 S UM DM SS SS U Acetic Anhydride S 0 0 0 0 0 0 0 Sd UU Sd U 0000 0 Acetone S U U UMMO US US MU S S Ms U Acetonitrile 0 0 0 0 0 0 0 OM U SM U 0 0 0 0 0 Acetylene S 0 0 0 0 00 085 0 S S U 0 0 0 0 0 Adipic Acid 0 0 0 0 0 0 0 08 S St S S 0000 0 Alanine S 0 0 0 0 0 0 085 S U S U 0 0 0 0 0 Allyl Alcohol 00 U 0 S 00 UO S SS 00 8 0 Aluminum Chloride 0 0 8 8580 3505385 85 8 8 S O US Ss S O Aluminum Fluoride 0 0 0 00 85 0 85 5U 5 0 0 8 0 0 Aluminum Hydroxide s 0 0 0 0 0 0 0 85 M Sd Ss Ss 0 0 0 0 0 Aluminum Nitrate MO 0 00 0 0 O S 0 0 S 0 0 0 0 0 0 Aluminum Sulphate s 0 0 0 0 0 0 0 Sd 0 S Sd 0 0 0 0 0 O Amino Acids S 0 0 0 0 00 085 SS 850000 0 Ammonia S 0 0
45. mination and elimination of the handled materials Users should be aware on the internationally recognized Laboratory Biosafety Manual which gives information on decontaminants their use dilutions properties and potential applications If decontamination procedures require the use of warming i e autoclave the rotating equipment should always be completely disassembled before being subjected to heat and after external chemical cleaning Seals Tubes and plastic components should be decontaminated with the method most suitable for them which might not be the same as for rotating equipment Note that the black color on the surface of the rotating equipment will be gradually washed out if the rotor is regularly autoclaved or bleached This does not necessarily denote degradation on the anodization A Thermo Electron Corporation Thermo Electron Corporation Chapter 5 Hazard Precautions and Limitations of Use Any part which has been subjected to temperatures above 130 C must be discered For any other unscheduled cleaning the customer must contact the manufacturer to verify that the proposed method does not damage the appliance Thermo makes no claims as to the effectiveness of proprietary brands of decontaminating solutions IEC CL31 CL31R 5 6 6 1 Periodic Cleaning Daily Weekly Disinfection IEC CL31 CL3TR Chapter6 Service and Maintenance All cleaning operations should be performed
46. n This feature is in accordance with international safety standards O Thermo Electron Corporation When the load imbalance indicator LED 13 is illuminated the centrifuge run has been automatically ended due to excessive load imbalance The rotor will be decelerated to rest at which time the lid can be opened and the load can be re balanced When the lid is opened this indicator will go off IEC CL31 CL31R 4 3 Chapter 4 Instructions for Use Description of Certain Events with Respect to the Control System Start and acceleration phase 4 4 Set error During the run Stop and braking deceleration phase IEC CL31 CL3TR End of run When the start button is pressed the centrifuge will begin to accelerate the rotor At this point the run timer will start and the middle display will begin to show remaining run time The upper display will show either actual speed or actual maximum RCF values whichever is currently selected The lower display shows the given acceleration profile and the LED corresponding to the acceleration button is illuminated During acceleration the centrifuge automatically detects the rotor type and therefore its maximum allowed speed If the operator sets a speed exceeding this the centrifuge stops acceleration when the maximum allowed speed is reached ensuring the operator s safety The display will blink and an audible alarm will invite the operator to correct the speed sett
47. ne 0 00 00 00 0U 0 0 U 0 0 0 0 0 0 Triethylene Glycol 0 0 0 0 0 0 0 0 8 Sd S ss 0 0 0 0 0 Tris Buffer neutral S 0 0 00 0 0 0 8 8 85 5 8 0 S S 0 O0 Trisodium Phosphate 0 0 50M 00 8 0 S 0 0 8 S S S Triton X 100 S 0 0 0 0 0 0 085 S S S S 0 S MO O Turpentine S 0 0 0 0 0 0 0 Sd Mt St Sd U O 0 0 O 0 Undecyl Alcohol 0 0 0 0 0 0 0 0 Sd Sd St Sd M 00 0 0 0 Urea MoO S S S 00 SS SS S S SS S S 0 Urine 00S 08 00 8 8 88 800 S S 0 Vinylidene chloride 0 0 00 0 00 OU UU U U 0 0 0 0 0 6 9 IEC CL31 CL31R Thermo Electron Corporation Chapter 6 Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Xylene SU P OM SO U U U U U U S S S Zinc Chloride M S S 0 0 00 S S Ss S SSMS SS S Zinc Hydrosulphite U 0 0 0 0 00 00 0 0 0 0 0 0 0 0 0 Zinc Sulphate U 0 0 0 0 0 0 0 Sd 0 S Sd 0 00 00 0 This table is intended as a guide only because of the difficulty in quantifying cross checking and monitoring the results under different conditions of temperature pressure and purity relating to the solvents and samples dissolved therein It is strongly recommended that you carry out your own trials particularly before protracted work periods Thermo Electron Corporation IEC CL31 CL31R 6 10 Certificate of Decontamination and Cleaning Certificate of Decontamination and Cleaning parts of it to Thermo Service Dept or to any Authorized Technical Assistance Service E Read carefully
48. ng Thermo strongly recommends that all operators comply with the local laws and regulations on safety and health in the workplace The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase professional handling assistance is required A e Unpack the centrifuge carefully removing any possible accessories and the material supplied for ordinary operations and maintenance e Check the contents of the package using the packing list provided above e Keep the packaging until the centrifuge has been tested and found fully functional Thermo Electron Corporation Positioning Mains Supply Lid Opening and Rotor Checking To install the rotor Thermo Electron Corporation Chapter 3 Installation The machine must be installed in a dust and corrosion free room Leave a 30 cm 12 in space free on each side of and behind the machine for safety reasons proper ventilation and maximum cooling performances Place the centrifuge on a bench top which must be rigid horizontal and sufficiently strong to support the centrifuge s weight and small vibrations Check mains and frequency they must correspond to the values shown on the instrument identification label For your safety check that mains wiring is effectively grounded Thermo declines all responsibility for any damages due to non grounding of the machine A Remember that in order to respect the electric
49. ng close the lid and wait 3 3 IEC CL31 CL31R Thermo Electron Corporation Chapter 3 Installation Figure 1 Insert the unlocking tool horizontally into the hole on the right hand side of the instrument Figure 2 While pressing up with your finger to press down with the unlocking tool pull the lid lever toward you the lid opens upwards The protection sticker must be replaced after it has been pierced item N 85241772 APRES Percer ici avec l outil f NS Pierce here With tool Se EN M ENS os Se Se Thermo Electron Corporation IEC CL31 CL31R 3 4 Chapter 3 Installation Performance and Accessories Note The performance figures indicated below are those of the rotors Their use at these speeds necessitates that the sample containers can support the corresponding forces T41 High Throughput Swing Out Rotor Cat No Description Container Capacity Capacity Max tube Maxtube Radius Maxspeed Max RCF Volume Insert Rotor 8 mm ht mm mm 11210435 141 rotating head 4100 11210436 Set of 4 standard buckets 77 11175712 Set of 4 sealing lids for 11175711 11175763 11175763 Set of 4 buckets 5 7 mL vacu 15 60 135 165 4100 3101 11175752 Set of 4 buckets 50 mL con 4 16 33 120 169 4100 3176 11175759 Set of 4 buckets 50 mL con 3 12 33 120 169 4100 3176 Self Standing 11175713 Se
50. o JOIN O O lo DIO c Oo o 2 o o o O c lao On IS O O olola c O o c O o CO Oo 2 o o wv o o o S Eee S S elim 2 Z2I2 i z 2 DD Z ZE l HID 2351515 5151335 D DIE l h gt OO o o o 2 o o o COO Oo o o o o o o olov olola O lo o ojojD o jojDl o jol o ojojojo jo o o o lo O o o o o o o o o O Oo o o 2 o ojo vV x 2 lt lv o 2 jo lo Se ce c o ES s o SID IO _ lo jololo Izl 2 E TL LI lt lt S 2 2 jejeje I6 B 5 S 238 83 83 l2s ls sels lt x x e elelele ls o s 5 5 DIDOD D IDO DICICTCITCIC DL jo jo jo jo jo jo jo jo Ss 0 0 S 0 S 0 S S Ss Ss S 0 S U 0000 8 S 0 0 Phenyl Ethyl Alcohol 0 S S S 0 S OM S SU Phosphoric Acid 10 v jo lolo jolo gt jojojojojn c o o jojo co o o jojo we gt jojojojo jm o ze o o mon lo c a 2 en jo co IID l 2 o lo O a 2 8 la jan jo Oo o o o o ly Oo o o o o co o o o o gt pojojojojo gt jojojojo jm zo lolo lol gt pjojojojojo on ol p gt 2 D 2 ele 2 E a e 1215 lt s ja lo eS SEE o al a Slo I Olalala lzosssg L9 elejlojo Ee fa as jo Oz 0 S 0 S S S S S S 0 SS S S 0 SS SS U S M 0 Potassium Chlorate 0 M S S506 S S Ss S U S S US SOSS U 0 0 0 0 00 05 UM SMU Potassium Chloride S S 0 0 Potassium Hydroxide 5 M
51. orrect radius value is in the control system the RCF value displayed will not be accurate Set run time When the LED next to TIME 20 is illuminated the middle display shows time values To increase decrease the run time values press on the corresponding up down arrows Set centrifugal radius 4 2 IEC CL31 CL3TR When the LED next to RADIUS 21 is illuminated the middle display shows the value of the cen trifugal radius in millimeters To increase decrease the centrifugal radius press on the correspon ding up down arrows This parameter can only be viewed and set while the centrifuge is at rest It is essential that this value be correct for the RCF control display function to be accurate The cor rect value of the centrifugal radius depends on the combination of rotor buckets adapters being used Thermo Electron Corporation Function Set acceleration profile Chapter 4 Instructions for Use Keystroke sequence and description When the button 8 is pressed the lower display 17 shows the number corresponding to the acceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down arrows Set deceleration profile When the button 9 is pressed the lower display 17 shows the number corresponding to the deceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down
52. r Rotor Removal Thermo Electron Corporation Chapter 6 Service and Maintenance rinsed off of metal parts and the parts dried It should never be used if there is surface damage to the rotor Formalin 3796 formaldehyde in water in contact for 10 minutes has a similar effect to chlorine bleaches Rotors should be thoroughly rinsed under running water for 5 minutes to remove all traces of formalin then dried completely Formaldehyde is toxic Gluteraldehyde 296 sold under many brand names such as Cidex and Glutarex requires total immersion for 10 minutes to ensure sterility Thorough rinsing and drying is essential to protect users Gluteraldehyde builds up to a toxic level in the fatty tissues of the body Phenols are very corrosive and should never be used A We recommend that all radioactive contamination be referred to your Radioactivity Safety Officer Rotors may be decontaminated by a mixture of equal volumes of Distilled water SDS diluted to 10 Ethanol diluted to 7096 The rotor should then be rinsed with ethanol followed by distilled water and then dried completely Thermo makes no claims as to the effectiveness of proprietary brands of decontaminating solutions If the main circuit breaker of the centrifuge cuts off the power to the instrument do not attempt to switch it on before a Thermo Service representative has checked over the unit To remove the rotor from the motor shaft Press
53. r source A view port located on the lid allows the measurement of the actual speed by a phototachometer Once a year check for correct r p m readout and speed control setting which should be within 100 r p m of the actual The CL31 centrifuge and its associated rotors offer a high level of RCF performance In some cases the maximum possible RCF will exceed the one sustainable by the sample container The rotor RCF performance displayed is the maximum available in the rotor pockets Depending on the sample containers or on other application needs the maximum RCF which must be used is to be determined by the operator The centrifuge is not explosion proof Using this centrifuge with explosive samples is entirely at the user s own risk Do not under any circumstances use the centrifuge in an explosive environment Due to the action of the turbulence created in the bowl a centrifuge is an aerosol generator A ventilated centrifuge like the CL31 exhausts into the room air which has passed through its bowl Thus the risk of the spread of aerosols is significant in a ventilated centrifuge In the case of samples presenting a biological or chemical risk the operator must take suitable precautions to prevent or reduce this risk IEC CL31 CL3TR 5 2 Chapter 5 Hazard Precautions and Limitations of Use 5 3 Duration of aerosol presence A Mechanical Risks Corrosion Information IEC CL31 CL3TR Thermo recomm
54. ratory use They will separate the components of fluids into layers of varying density by subjecting them to high forces Centrifugal force provided by the centrifuge can also be used to drive solvents and low molecular weight solutes through the membrane of a filtering device Retained macrosolutes will therefore be found above the membrane IEC CL31 CL31R 1 1 Chapter 1 Use and Function Refrigeration Systems 1 2 Condensation drain on refrigerated units IEC CL31 CL3TR Relative Centrifugal Force RCF generated by a rotor is directly proportional to its sedimentation useful radius and to the value of its speed squared A control system permits the user through an extremely intuitive control panel to set and control the speed the g force the temperature on the thermostated and refrigerated versions and the run time as well as to view different messages and warnings The CL31 is a ventilated unit A permanent air circulation reduces the heat level in the bowl However the temperature in the bowl can reach 10 C above the ambient temperature after hours of continuous operation The CL31R is equipped with a powerful refrigeration system that allows samples to be processed at low temperature The refrigerant R134a is free of CFC Chloro Fluoro Carbons in accordance with the Montreal Protocol directions for preservation of the ozone layer in the atmosphere The actual temperature in the bowl is continuously disp
55. rically opposite sides If the message persists despite your efforts to balance the load call your Thermo Service representative Note Imbalance tolerance depends upon the rotor in use The centrifuge will tolerate 10 gr of imbalance with the 4 x 280 mL rotor equipped with standard buckets Carefully balance the sample load to avoid actuating the imbalance detection system Relative Centrifugal Force RCF at the circumference of a rotor and bucket combination is directly proportional to the speed r p m and radius of the rotor Therefore a greater r p m and or a larger radius produces a greater RCF and improved faster separation of substances The centrifuge control system carries out and displays the results of all calculations related to speed radius and RCF Note The value introduced for the radius can be adjusted to allow for position within the tube such as at a boundary Maximum radii are quoted in the specifications tables Use of improper radius will adjust the speed setting automatically applying the wrong RCF A Legend R radius in millimetres N speed in r p m 1000 RCF gravitational acceleration g M add to memory MR memory recall Note To calculate actual results press the keys on a pocket calculator in the order shown Thermo Electron Corporation Chapter 1 Use and Function Primary calculations Key sequence not valid for CASIO HP RCF x g 1 118 R N Nx x
56. t of 4 inserts 280 mL 1 4 64 113 161 4100 3026 11175714 Set of 4 inserts 250 ml flat 1 4 61 112 161 4100 3026 11175715 Set of 4 inserts 200 mL 1 4 55 110 156 4100 2932 11175716 Set of 4 inserts 175 ml 1 4 60 120 166 4100 3120 11175717 Set of 4 inserts 135 mL 1 4 43 121 161 4100 3026 11175718 Set of 4 inserts 110 mL 1 4 40 122 158 4100 2969 11175719 Set of 4 inserts 100 mL 1 4 38 123 161 4100 3026 11175720 Set of 4 inserts 65 mL 2 8 34 104 156 4100 2982 11175722 Set of 4 inserts 50 mL con 2 8 29 114 134 4100 2518 11210348 Set of 4 inserts 50 ml Con 3 12 29 114 134 4100 2518 11175721 Set of 4 inserts 50 mL 4 16 28 5 106 132 4100 2481 11175742 Set of 4 inserts AMICON 1 4 28 135 160 4100 3007 Centriprep 11175723 Set of 4 inserts 38 mL 5 20 25 5 102 157 4100 2951 11175724 Set of 4 inserts 25 mL 5 20 24 104 155 4100 2913 11175725 Set of 4 inserts 25 mL Corex 4 16 24 07 155 4100 2913 11175726 Set of 4 inserts 16 mL 9 36 18 107 160 4100 3007 11175727 Set of 4 inserts 15 mL Corex 12 48 17 107 160 4100 3007 10 mL vacu 11175729 Set of 4 inserts 15 mL con 7 28 7 07 161 4100 3026 11175730 Set of 4 inserts 15 mL 4 16 7 119 163 4100 3063 11175731 Set of 4 inserts 15 mL 5 20 7 116 163 4100 3063 11175747 Set of 4 inserts 14 mL 4 16 15 5 121 163 4100 3063 Corning 11175732 Set of 4 inserts 13 mL 12 48 16 115 160 4100 3007 11175728 Set of 4 inserts 10 mL LP 9 36 16 12 160 4100 3007 11175733 Set of 4 inserts 8 mL 19 76 12 104 161 4100 3026 11175734 Set of 4 inserts
57. the unlocking device stored in the clip on the side of the centrifuge into the centre of the rotor and then lift out the rotor using both hands Replace the device in the clip IEC CL31 CL31R 6 2 Chapter 6 Service and Maintenance Trunnion Lubrification 6 3 IEC CL31 CL3TR Sealed rotors may be removed with the lids still in position and moved to a safety cabinet for manipulation of hazardous material This operation is necessary to allow the buckets to swing freely Clean the trunnions regularly with a dry wipe as well as the part of the bucket that rotates on the trunnions Then put a very small quantity of grease on the curved face of the trunnion Do not apply too much grease because it will eventually coat the bowl of the centrifuge as centrifugal force pulls the grease from the trunnions If the centrifuge is having imbalance problems try this operation before calling Thermo service In many cases imbalance problems arise due to the fact that poorly maintained bucket trunnion interfaces prevent the buckets from swinging freely A Thermo Electron Corporation Chapter 6 Service and Maintenance Chemical Compatibility Table for Rotors Tubes and Accessories S Satisfactory AL Aluminum BN Buna N D Discoloration but OK CAB Cellulose acetate butyrate CN Cellulose Nitrate P Pure chemical OK DL De

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