Precision Low Temperature Incubator User Manual
Contents
1. J2 14 3176707 3176720 J2 1 2 3 4 65232 Flat PCB Washer Control mns Lock Panel cy Washer 4 111 M NL P Spacer Screw SUGGESTED MOUNTING Your incubator requires the selection of 3 parameters for communications e Baud Rate This is the speed of communication between the incubator and computer Whatever is selected for the incubator must also be selected in the communication software selected to be used e Status Update Time Interval This is the time between information being sent from the incubator until it is received by the computer Status Display Type Selection of the format of data to be sent to your computer Read the following steps before performing any of them It will allow you to familiarize yourself with the procedure and to determine the values you want beforehand Note In the following setup mode there is a five 5 second time out feature that is active following each entry If the five 5 seconds is exceeded the unit will return to the normal operation mode If the time out occurs before the value was entered start over A Thermo Scientific Set Up the Incubator for Communications cont Thermo Scientific Section 6 Factory Options With the incubator ON simultaneously press and hold2. COMPRESSOR STARTING RELAY ONE SIDE OF POWER LINE H LMH YELLOW 12 LD TEP OVERLOAD PROTECTOR ORANGE _ BLACK BLACK _ WHITE 111 128 VOLTS HEATER 550W UNE us YELLOW WHITE P N 230 VAC ra 50Hz WHITE 9 2 Refrigerated Incubator Thermo Scientific thermoscientific com 2014 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 Thermo SCIENTIFIC A Thermo Fisher Scientific Brand
3. High Temperature Alarm Set Point Low Temperature Alarm Set Point Alarm Condition SET HIGH LOW ENTER TEMP CAL MUTE Refrigerated Incubator 1 3 Section 1 Installation and Start Up Explanation of Front Panel Controls cont Explanation of Auxiliary Panel 1 4 Refrigerated Incubator Set Temp Cal Key This key is used to view the operating setpoint tem perature or calibrate the unit to a certified traceable thermometer The first depression of the key selects the operating setpoint and the second depression selects the calibration mode The respective green indicator lamp will light Enter Key This key is used after any setpoint selection is made to enter the value into memory Up Down Arrow keys These keys are used to increase or decrease the desired setpoint value On the left side of the incubator control panel there is an auxiliary panel which has the items shown in the illustration below They are described as follows 1 Fuse This fuse is in line with the main power cord that comes into the incubator The rating of this fuse is printed above the fuse holder The physical size of this fuse is 5mm x 20mm 2 Alarm Relay This Alarm Relay output is provided to the user for the purpose of remotely monitoring the incubator in case of a high or low temperature alarm condition This alarm relay will operate just as the audible alarm would The contact itself is an isolated form C nor
4. Thermo Scientific Precision Model 815 Refrigerated Incubator Microprocessor Controlled Operating and Maintenance Manual 7013721 Rev 0 Visit us online to register your warranty Thermo www thermoscientific com labwarranty SCIENTIFIC Preface Units covered in this manual Catalog Model Numbers Volume L cu ft Voltage PR505755R 3721 566 20 115 PR505750R 3722 566 20 230 PR505750R CN 3722CN 3723 566 20 230 Manual Number 7013721 0 40096 4 3 15 Original new cabinet cooling On Off switch model numbers CCS Rev ECR ECN Date Description By Thermo Scientific Refrigerated Incubator i Preface A CAUTION Contains Parts and Assemblies r aA Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Warning If the incubator is not used in the manner specified in this operating manual the protection provided by the equipment design may be impaired Warning Depending on ambient temperature and settings surface can become warm to touch up to 60 C Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo
5. unplugging it from plug J302 The resistance between contacts at vari ous temperatures are as follows TEMPERATURE RESISTANCE QE Lu cn des etui E 100 OHMS DONE e s Ph e ease 108 OHMS BE auda n EE Es 114 OHMS JU CU Cue dia tn val conn rake 119 OHMS Note Only 2 of the 3 connector positions are used 4 Malfunctioning RTD Temperature probe The Control Board can detect if the temperature probe or the connec tion of the temperature probe to the circuit board is OPEN or SHORTED These two conditions are shown on the display as fol lows DISPLAY CONDITION PCT OPEN CIRCUIT SHORT CIRCUIT Before coming to the conclusion that the probe is bad when one of these displays appear check the connection of the probe to the circuit board 5 RTD Temperature Sensor Probe Location The Proper RTD location is important The RTD is supported by the stainless steel bracket attached on the rear wall and near the top of the chamber Two tube shaped clips securely support the RTD probe into place The RTD must be inserted fully into its support bracket Constantly Experiencing Low temperature Alarm If the incubator is constantly going into low temperature alarm then most likely the setpoint temperature setting is too close to the low temperature alarm setpoint setting The setpoint temperature setting should be a
6. for gases and 5 for humidity Our service personnel can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services 1 electrical service connection 2 tubing connections 3 gas regulators 4 gas tanks 5 unit leveling 6 room ventilation 7 adverse ambient temperature fluctuations 8 any repair external to the unit Damage resulting from accident alteration misuse abuse fire flood acts of God or improper installation Repairs to parts or systems resulting from unauthorized unit modifications Any labor costs other than that specified during the parts and labor warranty period which may include additional warranty on CO sensors blower motors water jackets etc Thermo Scientific Refrigerated Incubator V Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Section 8 Table of Contents Introduction mn dr ee exa Y 1 1 Unpacking and 1 1 Performance Wata iss elt ER com Sle a AE tmu a a 1 2 Theory of Operation te utn de Ha Pr C 1 2 General Informatio
7. None 0 Data Bits Eight 8 Stop Bits One 1 COM Port Selected by user Format ASCII Using the Communication The incubator transmits information on its RS232 port as long as the status update time interval is not 0 0 If you start your communication software with the incubator on most likely you will see the error s messages as follows ERR VALUE IS READ ONLY ILLEGAL COMMAND Wait for the Status Update Time duration you selected to complete and the communications will correct itself Besides having the incubator transmit information in one of the three formats the user can monitor other values or even change some of these from their computer Thermo Scientific Refrigerated Incubator 6 5 Section 6 Factory Options Using the Communication The following table lists the available commands for monitoring or continued controlling your unit view Description Range Change Command pion ge Command Actual Temperature 10 to 50 Read Only Setpoint Temperature 10 to 50 T10 thru T50 p pee EC Status Display Type DO thru D2 Display the Alarm Status Read Onl To View Data If Status Update Time is 0 0 no data will automatically be displayed To view data within a particular command code type that command code followed by the question mark symbol and then ENTER Return Example To view Setpoint Temperature type T CR To Change Data To change the value within t
8. avoid nuisance alarms caused by door openings Refrigerated Incubator 3 3 Section 3 Operation and Calibration PREVENTIVE MAINTENANCE Incubators Your equipment has been thoroughly tested and calibrated before shipment Regular preventive maintenance is important to keep your unit functioning properly The operator should perform routine cleaning and maintenance on a regular basis For maximum performance and efficiency it is recommended the unit be checked and calibrated periodically by a qualified service technician The following is a condensed list of preventive maintenance requirements See the specified section of the operating manual for further details We have qualified service technicians using NIST traceable instruments available in many areas For more information on Preventive Maintenance or Extended Warranties please contact us at the number listed below Cleaning and calibration adjustment intervals are dependent upon use environmental conditions and accuracy required Tips for all incubators Do NOT use bleach or any disinfectant that has high chloros Do not use powdered gloves for tissue cultures e Use sterile distilled or demineralized water 401 Millcreek Road Box 649 Marietta Ohio 45750 USA 740 373 4763 USA and Canada 800 438 4851 e Telefax 740 373 4189 http www service led marietta thermofisher com Preventive Maintenance for Model 815 Incubators
9. is the carrier s responsibility If you follow the above instructions carefully we will guarantee our full support of your claim to be compensated for loss or concealed damage Note Do not for any reason return this unit without first obtaining authorization In any correspondence to Thermo supply the nameplate data including catalog number and serial number Refrigerated Incubator 1 1 Section 1 Installation and Start Up General Information The incubator is commonly referred to as the standard model 815 The Model 815 Refrigerated Incubator has been designed to provide the ultimate in temperature control The user is assured of dependable and precise performance through the use of microprocessor technology in the temperature control circuitry This incubator is operable in the range of 10 to 50 C These temperature ranges will meet a wide variety of applications such as BOD determination general incubation as well as the preservation of critical materials Performance Data Uniformity values Temperature stability Heat up time Cool Temperature deviation Maximum temperature down time from Energy from set value spatial difference at chamber ambient temperature 27 point measurement center point 1 point P according to DIN according to DIN 12880 measurement 12880 0 Uniformity values Temperature deviation temperature from set value spatial 3 point measurement Recovery time after
10. minimum of 2 above the low temperature alarm setting If the problem persists then see Solution 3 Constantly experiencing high temperature alarm If the incubator is constantly going into high temperature alarm then most likely the setpoint temperature setting is too close to the high temperature alarm setpoint setting The setpoint temperature setting should be a minimum of 2 below the high temperature alarm setting If the problem persists then see Solution 3 Thermo Scientific Possible Solutions Thermo Scientific Section 4 Service Note Software version 2 00 and later has a 30 minute delay for the lowe continued 224 high alarm to avoid nuisance alarms caused by door openings 8 9 Ice buildup on evaporator coils Excessive frost buildup is the result of the introduction of outside moisture This may be caused by the evaporation of liquids placed in the chamber or it may be the result of excessive opening of the door during high humidity conditions The door gasket should also be examined for proper seal If repairs are necessary contact Technical Services Excessive line voltage variation Incubator will operate satisfactorily over full temperature range 10 C to 50 C when line voltages vary from nominal voltage by 5 or 10 Larger voltage variations may impair the Temperature Control Correct as required 10 Excessive ambient temperature variation If the incubator is placed close t
11. the UP and DOWN keys then press the ENTER key The letters will be shown in the display A number other than 96 might be shown in the display Choose the number appropriate for the Baud Rate desired 3 300 6 600 12 1200 24 2400 48 4800 96 9600 Press the UP or DOWN key until the display shows your selection Press ENTER The baud rate has been entered The display should change to the following Again the number in the display might be different than 0 1 but it will have a decimal point Choose the number appropriate for the Status Update Time Interval desired 0 0 Minutes No report 0 1 Between reports 0 5 Between reports 1 0 Between reports 2 0 Minutes Between reports 3 0 Min tes o esce eS siao ness Between reports 10 0 Minutes 30 0 Minutes 60 0 Minutes egret Between reports et aid Between reports piura xe ore Between reports Press the UP or DOWN key until the setpoint display shows your selection Press ENTER The value is entered Refrigerated Incubator 6 3 Section 6 Factory Options e incuDator 5 e display should have changed to the following Again the number Set Up th bator f The display should h hanged to the foll g Ag h b Communications cont in the display might be different than 1 This selection is for Status Display Type
12. Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2015 Thermo Fisher Scientific All rights reserved ii Refrigerated Incubator Thermo Scientific Preface T Important operating and or maintenance instructions Read the accompanying text carefully In Potential electrical hazards Only qualified persons should perform procedures associated with this symbol 77 N Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Si Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo Scientific products will be available at www thermoscientific com VY Always use the proper pro
13. Listed are the numbered selections with explanations and examples of the values displayed on your computer s monitor when the incubator transmits information 0 Used for a raw one line status output suitable for importing into a spreadsheet 1 Used for a multi line format with English headings with continuous screen 2 Used for the format as 1 but preceded by a clear screen command used by terminals or computers running terminal software This mode overwrites the previous transmission With Status Display Type 0 10 50 0 Where 10 Actual Temperature 5 0 Setpoint Temperature 0 Normal With Status Display Type 1 or 2 Actual Temperature 10 Setpoint Temperature 5 0 Alarm Status Normal LOW HIGH 4 Allow the setup to time out The incubator is now ready to communicate with your computer 6 4 Refrigerated Incubator Thermo Scientific Section 6 Factory Options Setting up the Computer Your communications software will most likely have a setup routine to set for Communication the parameters listed below These are the values the incubator is using so the computer parameters must match accordingly Also you will probably have to select the COM serial port you chose when you connected the cable from your computer to the incubator The software may ask what format the data is in You should choose ASCII Parameter Settings Baud Rate Baud Rate value selected earlier in Step 1 Parity
14. M OUTPUT P N 3176720 OPTIONAL P N 3176804 KSEE NOTE CABLE ASSEMBLY P N 3177654 GREEN 1 CONDUCTOR OF ORIGINAL LINE CORD RIBBED INSULATION BLACK A WHITE WHITE E WHITE COMPRESSOR STARTING RELAY YELLOW EY OVERLOAD eS PROTECTOR ORANGE BLACK BLACK WHITE WHITE HEATER 550 THERMAL CUTOFF LINE YELLOW WHITE P N 3175264 120 VAC o 60 HZ WHITE Thermo Scientific Refrigerated Incubator 9 1 Section 9 Electrical Schematics 815 ASSY PCB PWR SUP P N 3176814 ASSY PCB LIMIT ALARM P N 3176813 ASSY PCB CPU P N 3176707 ASSY PCB DISPLAY P N 3176815 J304 J305 2 2 1 TEMPERATURE Woe Jes Q Cie Ti BLACK Si rans 9 19 91 y 19 9 I E HHen SER gt Ol 2 8 5 COOLING eld No om SLEEVED MARKER MUST BE CONNECTED ON THIS END TO AUX D A FUSE r3 5 i 2 CERTE les io 3 5 20 V5 45 55 hae 65232 AUX D A I P N 3176720 P N 3176804 x L k SEE NOTE gt gt OPTIONAL LINE VOLTAGE a D ASSEMBLY IE ae ie TERM o 5 a P N 3177654 I 3 lt a 8 a m 5 2 1 CONDUCTOR OF ORIGINAL LINE CORD 2 RIBBED INSULATION 2
15. P 3176814 ASS Y PCB 815 HI LIMIT 3176813 ASS Y RTD TEMP PROBE 100 3176738 COOLING SWITCH POWER SWITCH 3175318 ASS Y CABLE INTERFACE 3176759 FUSE KIT 10 AMP 115V 3167218 FUSE HOLDER KIT 115V 3167216 FUSE HOLDER KIT 230V 3167217 CORD W PLUG 115V 3178034 HARNESS 815 818 115V 3177651 CORD W PLUG 230V 3176551 HARNESS 815 818 230V 3177652 FUSE KIT 5 AMP 230V 3167219 RS232 COMMUNICATIONS OPTION 3166245 THERMAL CUTOFF SWITCH 400141 STOPPER KIT 3167215 BULB KIT 3167220 MOTOR FAN REAR 130630 AUTO TRANSFORMER 230V 3174991 INS TEMP SENSOR BRACKET 316129 BACK HARNESS 3177654 REAR COMPRESSOR COVER 1900381 Thermo Scientific Refrigerated Incubator 8 1 Section 8 Spare Parts 8 2 Accessories Refrigerated Incubator Name Glass Thermometers RS232 Interface Kit Digital Chart Recorder Chart Paper 100 sheets Access Port Stopper Shelf includes fall protection strip and shelf mounting grommets Part Number 3173710 3166245 3166207 3174255 3167215 613185275 Thermo Scientific Section 9 Electrical Schematics 815 ASSY PCB PWR SUP P N 3176814 ASSY PCB CPU ASSY PCB DISPLAY P N 3176707 P N 3176815 TEMPERATURE PROBE J304 7 ON OFF switch BLACK SLEEVED MARKER MUST BE CONNECTED ON THIS END TO AUX D A 19 WHITE is el eH ASSY PCB AUX D A TBI De COM NC NO ALAR
16. Precautions Refrigerated Incubator The power cord of this instrument is equipped with a three prong grounding plug which mates with a standard three prong grounding wall receptacle to minimize the possibility of electric shock hazard from this apparatus The customer should have wall receptacle and circuit checked by a qualified electrician to verify receptacle is properly grounded Where a two prong wall receptacle is encountered it is the personal responsibility and obligation of the user to have it replaced with a properly grounded three prong wall receptacle Warning Do not under any circumstances cut or remove third ground prong from the power cord Do not use a two prong adapter plug Determine the total amount of current presently being used by other apparatus connected to the circuit that will be used for this unit It is critical that the added current demand and other equipment on the circuit not exceed the rating of the fuse or circuit breaker in use Caution Be sure the power supply is the same voltage as specified on the nameplate When loading the incubator a space of 1 2 must be allowed between adjacent items This will allow maximum air circulation which is necessary for proper temperature uniformity The uniformity will be adversely affected if air circulation is obstructed Liquid containers should never be placed in the incubator without covers The evaporation of moisture within the chamber will on
17. Refer to Action Daily Weekly Yearly Manual Section 3 Check temperature display versus setpoints w independent instrument v Inspect door latch hinges and door gasket seal v 3 Verify and document temp calibration as applicable See Calibration section v 5 Perform a complete decontamination procedure Wipe down interior shelves Between experiments and side panels with disinfectant Rinse everything well with sterile distilled More frequent decontamination water may be required depending on use and environmental conditions 3 4 Refrigerated Incubator Thermo Scientific Troubleshooting Symptoms and Possible Thermo Scientific Causes Section 4 Service Warning Service should be performed only by qualified service personnel Exercise care as line voltage is present in the control compartment A If refrigeration service is required after review of the Troubleshooting Section contact Technical Services In any communication with Thermo include the model and serial numbers from both nameplates Problems encountered with any constant temperature chamber will most frequently be related to temperature control Before proceeding with detailed troubleshooting be certain that the Low Limit and High Limit controls are adjusted properly If the limit controls are set too close or beyond the operating temperature the incubator may be consistently going into alarm This will result in erratic temperature control Firs
18. arance between the rear top and sides of the incubator and adjacent walls If two or more incubators are positioned side by side allow a minimum of 8 between cabinets Adjust the front leveling feet of the incubator so that the front is higher than the rear This will assist in door closing Choose a site free from rapidly changing ambient temperature conditions Wall mount brackets are attached to the top rear of the cabinet Remove any packaging materials from the brackets Rotate the brackets so they point straight out from the back Secure in place with the included hardware Attach brackets to wall Figure 2 1 Brackets During Shipping Figure 2 2 Brackets During Use The power cord is the mains disconnect Make sure the outlet and power cord are accessible at all times Caution The incubator should not be operated in an environment where the ambient temperature exceeds 90 F as the compressor thermo overload will be tripped and will result in a wide band control cycle of approximately 4 C Such cycling should not be misinterpreted as a malfunction of the electronic controls Radiators air conditioning outlets other ventilating system outlets and drafts can affect the operation of the incubator by a sudden inrush of air that is at a temperature different than operating conditions Warning For personal safety this apparatus must be properly grounded Refrigerated Incubator 2 1 Section 2 Calibration 2 2 Safety
19. c is told to operate when need be by the microprocessor Refrigerated Incubator 4 3 Section 4 Service 4 4 Possible Solutions continued Refrigerated Incubator There is another device known as an Optoisolator which serves as the high low voltage isolator between the triac and the microprocessor When the incubator starts experiencing temperature problems and or variations due to known controller malfunctions these two components become prime suspects The reason being they are under higher operating stress than other components If the incubator starts experiencing temperature problems such as no heat constant heat or creeping upward heat then follow the troubleshooting instructions below These instructions require the use of a voltmeter being able to measure DC and AC voltages and preferably a digital voltmeter Warning The following troubleshooting instructions require that power be on only qualified service personnel should perform these procedures A Remove the control cover and familiarize yourself with the power supply assembly 3176814 Locate the Triac Q2 the Opto isolator U1 resistor R4 Also locate the test point 2 TP2 The first measurements will be DC voltage measurement so a DC scale of at least 10 volts should be selected Connect the negative lead to TP2 This is DC ground Also locate the terminal block TB1 B This first section will be to verify the heater command is correct fr
20. continuous operation Warning Turn off power switch and unplug line cord from power source before starting any maintenance procedure If the chamber needs to be cleaned a mild soap and water solution or bicarbonate of soda 1tbsp gallon of water is recommended When the incubator is used frequently below ambient temperatures or in any manner that increases moisture buildup within the chamber an appropriate defrosting schedule must be determined Defrosting is accomplished by turning the incubator off for 24 hours Moisture will collect in the drip pan beneath the incubator General Cautions Overcharging a refrigeration system can cause excessive system pressure and can be dangerous 2 The compressor terminal protective cover must be properly in place before applying electric power to the compressor 3 Caution should be exercised by service personnel when servicing refrigeration system 4 Service should be performed by a certified refrigeration technician only Thermo Scientific Refrigerated Incubator 5 1 Thermo Scientific RS232 Section 6 Options Instructions to install and operate the RS232 option follow 1 Remove the eight 8 screws securing the control panel There are four 4 on the top and 2 on each of the sides Then remove the cover by sliding it towards the front of the incubator 2 Slide open the auxiliary panel door and locate the mounting area of the RS232 board as shown in illustration follo
21. door open ing according to DIN 12880 0 5 C 5 5 5 A lt 0 15 X ads Note The above performance figures are based upon the following operating conditions Ambient temperature of 22 C 3 C Line voltage 115V 5 115V version and 230V 5 230V version The following test protocols have been used 3 point measurement test 1 thermocouple sensor in center of each shelf 1 shelf top position 1 shelf lowest position 1 shelf middle position 27 point measurement test 9 x thermocouples on each shelf 1 shelf top position 1 shelf lowest position 1 shelf middle position based on DIN 12880 1 point measurement test 1 thermocouple at chamber center point temporal temperature variation characterized by the maximum temperature difference 1 2 Refrigerated Incubator Thermo Scientific Theory of Operation Explanation of Front Panel Controls COOLING On Off gt AMBIENT 5 C Figure 1 1 Front Panel Thermo Scientific Section 1 Installation and Start Up The refrigeration system defrost heater and air circulating fan are used in conjunction with a microprocessor controlled proportioning circuit to achieve sensitive temperature control An RTD Resistance Temperature Detector located in the airstream senses any temperature deviation from the control point and heat is provided proportionally to maintain the desired temperature Regardless of what temperature is being maintai
22. e is 10 millivolt degree C When the incubator is operating at a negative temperature the chart recorder output is still positive Use the chart below to relate temperature to output voltage Temperature umet Degree C Millivolts RELAY um VOLTAGE CLASS 88 232 2 CINECTIIN ONLY Ko qe 9 Instrumentation Port Thermo Scientific Located on the chart recorder printed circuit board is an offset potentiometer R3 If for some reason the recorder output voltage does not match the temperature as shown in the chart this potentiometer can be used to tune it in When connecting a chart recorder to the recorder output connector it is recommended to use a shielded cable with the shield grounded at the chart recorder and to keep the cable short as possible Located on the rear side of the incubator is an instrumentation port for the user to insert sensor wires external meter leads etc into the chamber The rubber plug provided must always be used in this port to insure the uniformity specifications An extra plug is supplied for the user s convenience Be sure to seal any gaps around the wire s going through the plug Refrigerated Incubator 1 5 Thermo Scientific Section 2 Installation Use the proper lifting equipment when moving this unit The unit s shipping weight is 315 lbs 142 9kg To assure proper ventilation allow a minimum of 4 of cle
23. e operating SETPOINT TEMPERATURE the incubator alarm will activate Caution If the default high temperature alarm setpoint 1 C above setpoint is increased the new alarm temperaure value will remain valid until power is removed from the unit turning off the power switch unplugging or power failure When power is restored the alarm setpoint will revert back to the default To change the low temperature alarm setpoint After selecting a setpoint temperature the LOW TEMPERATURE SETPOINT automatically defaults to 1 lower than the setpoint that was entered This 1 default difference can be overridden if desired The following paragraph describes this Press the High Low Limit key twice The green Low Temperature Alarm Setpoint indicator should be lit Use the UP or DOWN arrow keys to select your limit value Then press the ENTER key If the LOW LIMIT value you have selected is above the operating setpoint temperature the incubator alarm will activate Thermo Scientific Thermo Scientific Section 3 Preventive Maintenance Caution If the default low temperature alarm setpoint 1 C above setpoint is increased the new alarm temperaure value will remain valid until power is removed from the unit turning off the power switch unplugging or power failure When power is restored the alarm setpoint will revert back to the default Note Software Version 2 00 and later has a 30 minute delay for the low and high alarm to
24. ect from the microprocessor through the optoisolator and through the Triac when the incubator is requesting heat The steps in this section must be followed in succession 1 Select a setpoint temperature at least 10 above what the actual temperature is The incubator should be requesting heat as indicated by the Heater On indicator lamp on the front panel It should be on continuously not flashing 2 Measure the voltage at U1 Pin 2 with respect to TP2 It should be no greater than 4 volts DC 3 Measure the DC voltage across R4 since the incubator is requesting heat there should be current flowing through this resistor making the voltage drop equal to 3 volts 0 5 volts 4 If the last two steps are not as stated then most likely the CPU board is bad and should be replaced 5 Switch voltmeter to AC Volts scale capable of reading 120 volts 6 Measure the voltage between U1 Pin 4 and UI Pin 6 It should be less than 1 VAC 7 lfit is not most likely the optoisolator is bad and the power supply board should be replaced 8 Measure the voltage between TB1 4 Orange wire and TB1 6 Red wire It should be line voltage 110 VAC to 120 VAC 9 Ifit is not then most likely the triac is bad The power supply board should be replaced Refrigerated Incubator 4 5 Section 4 Service 4 6 Possible Solutions continued Refrigerated Incubator D Measure RTD temperature probe resistance at the connector after
25. ee 3 possible values 0 Normal no alarms 1 Low alarm 2 High alarm Thermo Scientific Refrigerated Incubator 6 7 Section7 Specifications External dimensions D x W x mm in 787 x 864 x 1956 31 x34 x 77 Internal dimensions D x W x mm in Loading capacity per shelf kg Ibs Power line voltage 5 frequency Humidity 80 RH at or below 31 C decreasing linearly to 50 RH at 40 C Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It Safety Specifications depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply Pollution Degree describes the amount of conductive pollution present in the operating environment Pollution Degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Measured at ambient temperature of 32 C 90 F Thermo Scientific Refrigerated Incubator 7 1 Section 8 Spare Parts DESCRIPTION PART NO ASS Y PCB DISPLAY 815 3176815 ASS Y PCB 815 CPU 3176707 ASS Y PCB 815 PWR SU
26. ference might be only a few tenth s of a degree Refrigerated Incubator 3 1 Section 3 Operation and Calibration 3 2 Refrigerated Incubator The CALIBRATE key should be used ONLY to match a stable incubator s actual temperature to the calibrated thermometer Place a calibrated thermometer or the probe of one at the center of the third shelf of the incubator Select the SETPOINT desired Allow the unit to run for at least an hour after it reaches the setpoint before calibration is performed Note If possible place the thermometer into a liquid for stability To perform calibration press the SET TEMP CAL twice The green calibration indicator should be lit Then press either the UP or DOWN arrow keys to make the display match the noted thermometer reading After the satisfied display is achieved press the ENTER key The display will then return to showing the newly calibrated actual temperature To change the high temperature alarm setpoint After selecting a setpoint temperature the HIGH TEMPERATURE ALARM SETPOINT automatically defaults to 1 higher than the setpoint that was entered This 1 default difference can be overridden if desired The following paragraph describes this Press the HIGH LOW LIMIT key once The green High Temperature Alarm Set Point indicator should be lit Use the UP or DOWN arrow keys to select your limit value Then press the ENTER key If the HIGH LIMIT value you have selected is below th
27. he command type the command code followed by the new value and ENTER Return Example 1 To change Setpoint Temperature to 25 0 C type T25 0 lt CR gt Example 2 To change Setpoint Temperature to 8 C type T 8 lt CR gt A Actual Temperature The actual temperature within the incubator T Setpoint Temperature The temperature at which the user has selected the incubator to operate 6 6 Refrigerated Incubator Thermo Scientific Section 6 Factory Options Using the Communication _ R Baud Rate The rate at which data is transmitted between the continued incubator and your computer 0 300 Baud 1 600 Baud 2 1200 Baud 3 2400 Baud 4 4800 Baud 5 9600 Baud S Status Update Time Interval The time between data messages sent from the incubator to your computer 0 0 Minutes No report 1 0 1 Minutes 6 seconds 2 0 5 Minutes 30 seconds 3 1 0 Minutes 4 2 0 Minutes 5 5 0 Minutes 6 10 0 Minutes 7 30 0 Minutes 8 60 0 Minutes V EPROM Version Number The incubator software version level D Status Display Type Selection of format that data is sent to your computer 0 Used for a raw one line status output suitable for importing into a spreadsheet 1 Used for a multi line format with English headings 2 Used for the format as 1 but preceded by a clear screen command used by terminals or computers running terminal software O Display the Alarm Status The alarm has thr
28. ly add frost and hasten the need for defrosting This chamber is not self defrosting Excessive frost buildup on the evaporator coil located on the lower rear wall will also affect temperature uniformity Do not place any explosive combustible or flammable materials in the chamber 2 Do not place sealed containers in the chamber Sealed containers filled with materials do not provide room for expansion and can develop dangerous vapor pressure as the temperature increases 3 Avoid spillage of liquids within the chamber 4 Do not evaporate noxious fumes Caution Do not store containers filled with acidic or caustic solutions as vapors from these materials will attack the evaporator and void the warranty amp Thermo Scientific Thermo Scientific Section 3 Operation and Calibration Power up by depressing the ON OFF switch Immediately after turning on power all the segments of the display and all of the indicators will be on After one second the display will show the actual temperature and the actual temperature indicator light will be lit If the setpoint temperature is greater than the actual temperature then the heater on lamp will be on or start to flash If it flashes then the actual temperature is near setpoint temperature To display any temperature value With the use of either the SET Temp Cal key or the HIGH LOW LIMIT key any temperature value can be displayed To view the set temperature press the set Te
29. mally open normally closed dry contact This contact is to be used for low voltage class 2 connections only The contact rating is 24 volts 1 25 amps resistive Typical usages of the output are shown below NC LIGHT NO VOLTAGE SOURCE In the above configuration the light will illuminate whenever the unit goes into an alarm condition High or Low In the configuration below the light will go off in a high or low temperature alarm condition LIGHT NC ZT NO VOLTAGE SOURCE L Warning Wiring must conform to all local electrical codes Thermo Scientific Explanation of Auxiliary Panel continued 3 4 Section 1 Installation and Start Up RS 232 Output Optional Kit P N 3166245 This output is used for two way communications between the incubator and a personal computer With the use of communications modem software program the user can record the temperature of the incubator at their selected time periods and store it in a file for use with a spreadsheet program The user can also change the setpoint temperature from their personal computer and periodically monitor the actual temperature setpoint temperature and alarm status Recorder Output This is a DC millivolt output which represents the temperature of the incubator The recommended main use of this is with a chart recorder having an input impedance of at least 1 megohm The scaled temperature output chang
30. mbly see Solution 3 Does not reach a high set point temperature No air circulation in chamber inoperative fan see Solution 1 Low line voltage less than 110V see Solution 9 RTD Temperature Probe see Solution 3 Step D and or Solution 4 Power Supply PCB Assembly see Solution 3 Overcharged compressor see Solution 11 Differences between Temperature Set and Digital Temperature Readout see performance figures page 3 RTD Temperature Probe see Solution 3 Step D and or Solution 4 Malfunctioning Power Supply PCB assembly see Solution 3 Takes an extremely long time to reach high temperature set point No air circulation in chamber inoperative fan see Solution 1 Low line voltage less than 110V see Solution 9 Power Supply PCB assembly see Solution 3 Overcharged Compressor see Solution 11 m tiriiritt Display indicates Malfunctioning RTD Temperature Probe Solution 4 4 2 Refrigerated Incubator Thermo Scientific Possible Solutions Thermo Scientific Section 4 Service 1 Inoperative Fan Open the door Place your hand near the grill at the top rear of the chamber and check for air circulation If there is no air circulation the fan motor is malfunctioning Contact Technical Services 2 Burned out heater To check this the control panel cover must be removed Warning Hazardous high voltage conditions exist inside control panel Turn OFF line switch on front panel and
31. mp Cal key once The green indicator for the set temperature will light If no other keys are pressed within 5 seconds the display will automatically return to the actual temperature To view the calibrate temperature press SET CAL twice while the display is showing the actual temperature The same sequence of key operation is true for the use of the HIGH LOW LIMIT key To change the setpoint temperature Press the SET TEMP CAL key once Then press the UP or DOWN arrow key until the desired temperature is displayed When continuously pressing either the UP or DOWN keys the display will begin to change rapidly With a little practice one will become accustomed to this After the desired set temperature is achieved then press the ENTER key The display will then return to showing the actual temperature If the ENTER key is not pressed the expected new set temperature will not be accepted and the incubator will control at the previous set temperature If the setpoint temperature is over ambient temperature by more than 5 C the compressor cooling function isn t needed Set the cooling switch at the position Then the energy and temperature performance will provide better values The incubator is calibrated at the factory for use over a wide range of temperatures Due to the slight non linearities in the control system it may be necessary to make the display match a calibrated thermometer s reading even though the dif
32. n 1 2 Explanation of Front Panel Controls 1 3 Explanation of Auxiliary Panel 1 4 Instrumentation Port seo eres Eae XC Ib eb xp e 1 5 Installation STEP URS tenes Suga 2 1 Safety Precautions deve SUIS bo y eet aka d EE EP 2 2 Operation and Calibration 3 1 asks ness DRE ERREUR QE ICE NE 4 1 Troubleshooting 22 tot cues dba Seu iei SE ER CE UR 4 Symptoms and Possible Causes 4 1 Possible Solutions ssa nunnana 4 3 Maintenance ivo cess eR RR RR RR ER ERE E e REOR QE ERI CK 5 1 General 5 1 Options Emm 6 1 cea Ao Wee eae be ede CEOS 6 1 Setting Up the Incubator for Communication 6 2 Using the Communication d 315 0022 CREER OR DRE RES 6 5 Setting up the Computer for Communication 6 5 Specifications 25 3 i Sheu testes td Eo ER rua VAS 1 1 Spare Parts ocio IE PLE 8 1 NOCESSONES ui nt oe ge piu eto ee RUE RE OEY NUR DU Ke RS S 8 2 Refrigerated Incubator vi Unpacking and Thermo Scientific Damage Section 1 Introduction Your satisfaction and safety are important to Thermo and a complete understanding of this unit is necessary to attain these objectives Warning As a routine laboratory precaution always
33. ncing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv Refrigerated Incubator Thermo Scientific Preface Warranty Notes Information You Should Know Before Requesting Warranty Service Locate the model and serial numbers A serial tag is located on the unit Itself For equipment service or maintenance or with technical or special application inquiries contact Technical Services at 1 800 438 4851 or 1 740 373 4763 USA and Canada Outside the USA contact your local distributor Repairs NOT Covered Under Warranty Calibration of control parameters Nominal calibrations are performed at the factory typically 1 C for temperature 1
34. ned the compressor operates continuously This constant operation alleviates component failures associated with cycle type operation The circulating fan provides even air distribution throughout the chamber and ensures temperature uniformity Heater On Lamp Illuminates when power is applied to the heater LED Display Four digits used to display the actual set calibrated high limit and low limit values Indicator Lamps There are five 5 green indicator lamps that support the parameter showing on the display The sixth indicator is for a high or low temperature alarm condition An internal audible alarm will also sound when this alarm indicator illuminates Power On Off Switch The Power On Off switch provides the main power to the incubator Cooling On Off Switch The On Off Cooling Switch provides better energy and temperature performance when setting a temperature greater than ambient 5 C When the setting temperature is greater than ambient 5 C set the cooling switch to Off position Mute Key This key is used to silence the internal audible alarm and to de activate the external alarm device if one is being used High Low Limit Key This key is used to view the high or low tempera ture alarm setpoint The first depression of the key selects the high limit The second depression selects the low limit The respective green indicator lamp will light Actual Temperature Set Temperature Calibration
35. o the air conditioning outlet hot radiators or direct sunlight or if the ambient temperature changes drastically the chamber temperature will be affected Correct as necessary Caution The incubator should not be operated in an environment where the ambient temperature exceeds 90 F as the compressor thermo overload will be tripped and will result in a wide band control cycle of approximately 4 C Such cycling should not be misinterpreted as malfunction of the electronic controls 11 Overcharged compressor This would happen only if compressor was overcharged after installation For correct charge see nameplate located behind lower portion of door If unit has more charge than shown it will not reach 50 C Compressor must be discharged and recharged correctly 12 The circuit design calls for continuous compressor operation If compressor cycles On Off it is an indication that the starting relay is overriding the control circuit This may be the result of excessive 90 F ambient temperature adjacent to the compressor excess dirt on the compressor or a relay malfunction Provide additional wall clearance and or circulation around the unit clean compressor or contact Technical Services Refrigerated Incubator 4 7 Section5 Maintenance The design of the incubator is such that periodic maintenance is kept to a minimum No lubrication is required The following paragraphs cover the few minor procedures necessary for
36. om the microprocessor through the optoisolator and through the Triac when the incubator is NOT requesting heat The steps in this section must be followed in succession 1 Select a setpoint temperature at least 10 below what the actual temperature is The incubator should not be requesting heat as indicated by the Heater On indicator lamp on the front panel It should not be on continuously or even flashing 2 Measure the voltage at U1 Pin 2 with respect to TP2 It should be no less than 4 volts DC 3 Measure the DC voltage across R4 since the incubator is not requesting heat there should be no current flowing through this resistor making the voltage drop equal to zero volts 4 If the last two steps are not as stated then most likely the CPU board is bad and should be replaced 5 Switch the voltmeter to an AC Volts scale capable of reading 120 volts Thermo Scientific Possible Solutions Thermo Scientific continued C Section 4 Service 6 Measure the voltage between U1 Pin 4 and UI Pin 6 It should be line voltage 110 VAC to 120 VAC 7 If it is not most likely the Optoisolator is bad and the power supply board will have to be replaced 8 Measure the voltage TB1 4 Orange wire and TB1 6 Red wire It should be approximately 0 VAC 9 Ifit is not then most likely the triac is bad and the power supply board should be replaced This next section will be to verify the heater command is corr
37. t the symptoms and possible causes will be listed for quick reference Solutions will be suggested later Warning Hazardous high voltage conditions exist inside the control panel unplug line cord and turn off line switch before removing the cover Only qualified electrical instrument personnel are authorized to perform troubleshooting and or servicing A Freeze up or gradual drop in chamber temperature No air circulation in chamber inoperative fan see Solution 1 Heater see Solution 2 Power Supply PCB Assembly see Solution 3 RTD Temperature Probe see Solution 3 Step D and or Solution 4 Side walls are getting warm Depending on ambient temperature and the specific settings the side walls may get warm to the touch up to 60 C 140 F This is not a malfunction This is normal due to the location of the con denser heat exchanger of the cooling system Note that it has no impact on the specified performance of the unit Refrigerated Incubator 4 1 Section 4 Service Symptoms and Possible Temperature variations at set point poor control Causes continued Excessive ambient temperature variations see Solution 10 Compressor Relay failure see Solution 12 No air circulation in chamber inoperative fan see Solution 1 RTD Temperature Probe Location see Solution 5 Ice buildup on evaporator coils see paragraph on Defrosting RTD Temperature probe see Solution 3 Step D and or Solution 4 Power Supply PCB Asse
38. tective equipment clothing gloves goggles etc VY Always dissipate extreme cold or heat and wear protective clothing v Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Scientific Refrigerated Incubator iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experie
39. unplug line cord Remove all screws that fasten the cover Locate nylon connector J1 on the Limit Alarm PCB Assembly Depress both ears on the plug housing and gently pull out the connector With an Ohmmeter measure the resistance between TB1 4 Orange wire and TB1 6 Red wire The proper heater resistance is 32 ohms If heater resistance is correct then measure between one heater lead RED or ORANGE and incubator ground it must be infinity Open If the heater resistance check indicates an open circuit it may or may not be open In series with the heater is a thermal overload switch It might be that this thermal overload is open and not the heater To check the heater and thermal switch directly the shelfs of the incubator and the back wall cover must be removed Unplug heater and measure its resistance If the resistance is correct check resistance between heater terminal and the heater sheath It must be an open circuit If the heater is good then check the thermal overload switch At room temperature it should be closed 0 ohms This switch should only open at 80 C and then close at 65 C If the heater and thermal switch are good then the problem is directed towards the heater wires in the harness If heater is defective contact Technical Services Malfunctioning Power Supply PCB assembly The Power Supply PCB Assembly has the triac solid state AC voltage switch on it which supplies the power to heater This tria
40. wear safety glasses when working with this apparatus This product is not intended nor can it be used as a sterile or patient connected device In addition this apparatus is not designed for use in Class I II or III locations as defined by the National Electrical Code Warning If this equipment is not used as specified in this manual the protection provided by the unit design may be impaired Save all packing material until unit is put into service This merchandise was carefully packed and thoroughly inspected before leaving our factory Responsibility for safe delivery was assumed by the carrier upon acceptance of the shipment therefore claims for loss or damage sustained in transit must be made upon the carrier by the recipient as follows 1 Visible Loss or Damage Note any external evidence of loss or damage on the freight bill or express receipt and have it signed by the carrier s agent Failure to adequately describe such external evidence of loss or damage may result in the carrier s refusing to honor your damage claim The form required to file such claim will be supplied by the carrier 2 Concealed Loss or Damage Concealed loss or damage means loss or damage which does not become apparent until the merchandise has been unpacked and inspected Should either occur make a written request for inspection by carrier s agent within fifteen 15 days of the delivery date then file a claim with the carrier since the damage
41. wing 3 Mount the RS232 printed circuit board assembly using the screws or standoffs provided See illustration below 4 Connect one end of the cable to J304 located on the left edge of the left printed circuit board p n 3176707 Be careful of alignment 5 Connect the other end of the cable to the cable connector on the RS232 PCB assembly see illustration 6 Replace the control panel and secure with the eight 8 screws The RS232 PCB provides a bi directional communication port that will allow the user to monitor the performance or the change the operating valves of the incubator from a remote computer RS232 Communication will require a communication program such as a modem program installed in your computer which will allow the storage of data within your files The RS232 PCB utilizes one of the users computer serial ports such as COMI or COM2 The communication software must be configured to the selected port The serial port is an IBM PC AT style port The cable that connects to it must end in a DB 25 25 pin male connector The cable is a one to one wiring format The pin assignments for the serial port are Serial Transmitted Data Serial Received Data Signal Ground O V Refrigerated Incubator 6 1 Section 6 Factory Options Setting Up the Incubator 6 2 for Communication Refrigerated Incubator 4304 Assy PCB 815 CPU 3176707 TOP VIEW OF CONTROL PANEL
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