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Gradient Pump

1. Part Number Description Remark Gradient Pump 1001012020 Analytical Carrier Assembly 1601123010 Pulse Damper Assembly 1001633430 Timing Belt 1602423150 In line Filter Assembly 1602423130 Prime purge valve 1602423270 Zirconium Plunger Analytical 1602424030 Outlet Check Valve Cartridge 1601823310 High Pressure Seal Kit 9311032000 Gradient Pump Main Board Assembly 1301032000 Gradient Pump Motor Power Board Assembly 1301032010 Gradient Pump Transformer Assembly 9311032010 Gradient Pump Terminal Board Assembly 1301032040 Middle Board Assembly 9311333030 Gradient Pump Keypad amp Silk 1301533210 LCD Module 20 x 2 1301533160 Cooling Fan 126LH 1301032020 Transducer and Cable Assembly 9311032020 Rinse Pump and Cable Assembly 1301032050 Photo Sensor Assembly 1602323160 Stepping Motor Young Lin Gradient Pump MAIN POWER BOARD mmorum Joa las nee 1 piwa mawn 1 1 pajos swana jor we jo s X 26 27 28 29 30 3 MAIN POWER BOARD 93 17 J942 BOSSE Ue ER ME 18 10 lt amp p 3 U RGT 1 20 CAUS KEN SA BA VIEW A 2 SET SCREW ZI ARAU ZUA SCALE NONE Fig 5 5 The drawing of the Gradient Pump carrier housing Young Lin Gradient Pump Fig 5 6 The drawing of the Gradient Pump UV Vis Detector User Manual Ah YOUNG LIN INSTRUMENT CONTENTS 1 Introduction 2A 1 1 Fun
2. New Password Yes 8020 Setup the password to input PID control value in order to track the oven temperature After passing identification you can edit the value The default password is 8020 Failure of identification displays Reboot No display for restarting the Column Oven 6 Input of PID constants K0 47 10 12 8 D0 3 2 T0 1 0 The column oven control oven temperature by digital PID The PID constants can be inputted from 0 to 160 degree by 16 degree segment K is proportional coefficient is integral coefficient D is differential coefficient and T is integration and differentiation time Young Lin Column Oven 4 Maintenance When any of error messages are appeared refer to table 2 and remove the cause Table 4 1 Error messages and their Cause D Solvent Leak Leak on the oven inside Remove cause of leak by replacing O S ien aeea oon Door Opened Door is opened during heater Decrease current temperature and orking or when door open limit close Flammable Gas Leak of volatile solvents by Open the door and ventilate inside and loosening fittings or in flowing remove cause volatile gases Various problems occurred during use are described in the Table 3 Table 4 2 Problems and their fixing Current temperature is Turn off and contact us over setting temperature Heating does not work Heater does not work 1 Replacement of fuse 1 Fuse out No LCD d
3. 12 Setup of proportional differential and integral control value for isocratic mode Display PID Set gt Gain 0 36 P 0 24 1 4 80 D 0 12 Input range 0 01 9 99 Input of constant values according to PID trace at isocratic mode Gain Setup for response gain If the input value is higher excessive response is increased and if the input value is lower response is slow P Setup for response proportional coefficient corresponding flow rate As Gain does Excessive response is increased and if the input value is lower response is slow l Setup for compensation between real pressure and setup pressure If the input value is higher hunting appearance is occurred and if the input value is lower stabilization time is increased D Setup for preventing from rapid excessive response If the input value is higher time for reaching integration is increased and the input value is lower is sensitive to external turbulence The values from 10 to 12 can not be changed on the normal Configuration mode These can be changed only on the QC configuration mode If you change these value the system can not work properly 4 5 ISOCRATIC MODE After switch on the Gradient Pump is automatically setup as an isocratic mode unless the Gradient Pump is previously used for a slave mode If succeed to setup as an isocratic mode following is displayed Flow Rate 000ml min A 100 B 0 C 0 Aand Wkeys are used for moving lines and the CLR SCL
4. so exact gradient ratio is transferred 2 5 Gradient curve The Gradient Pump can edit 10 gradient programs of 10 lines for gradient analysis Gradient curve whose shape is obtained by the following exponential equation may be selected and inputted for each line Gradient curve may be applied for both of gradient and flow rate If solvent B is introduced and mixed to solvent A at flow rate F and mixed solvent flows to column at flow rate Fap concentration of solvent B is c 100 x 1 Fab Fe over V x tPF 1 Fay Fp over V x t PF In the event that the above equation is actually applied the difference between gradient ratio of current line and gradient ratio or flow rate of next line is the form of the above exponential curve which 1 8 Young Lin Gradient Pump increases and decreases 2 6 Washing port When using buffer solution salts are generated on back side of high pressure seal and these deposits wear pump seal to cause shortening of seal life which has bad effect on pump Washing port enables to insert proper solvent in back side of high pressure seal to prevent salts from being deposited and activated Mixed solution 20 MeOH of water and methanol is used as cleaning solution and life of seal is extended with lubrication action in general analysis Young Lin Gradient Pump 3 Installation and preparatory test 3 1 Inspection and installation The Gradient Pump is delivered along with the follow
5. 1 1 Requirements In addition to the detector itself you will need the following items for setup and initial operation Autochro 2000 or Autochro 3000 Chromatography Data System 1 HPLC 2 Column 3 Standard test mix 5 Nuts ferrules appropriate to the column end fittings being used 4 Appropriate solvents reagents etc 6 Wrenches appropriate to column end fittings 7 Connecting tubing and union if column cannot be connected directly to the cell 2 2 Site requirement of the UV Vis Detector 1 Room with 20 C temperature with variation 5 C with and 60 humidity 2 Where no direct and straight sunlight 3 Plain floor without carpet 2 6 Young Lin UV Vis Detector 4 Where having spare space of 20cm or more 5 Where there are 10 or less voltage change no frequency change and 100 or less grounding point 6 Where there is no generation of corrosive gas and ventilation is well done 7 Where stable power of 110 or 220V is supplied 8 Where not receiving electromagnetic induction from large transformer high frequency heater UPS etc 2 2 1 Location Place the detector on a laboratory benchtop in close proximity to the HPLC column outlet Allow at least 5 inches of clear space between the rear panel of the unit and any wall or obstruction This provides both access to the rear panel connections and a free flow of air 2 3 Connections and control 2 3 1 Connections for power Ther
6. 6 Wavelength Scan mode The scan mode is used for getting absorbance spectrum from a sample From the absorbance spectrum you can get information for maximum absorbance wavelength After selection of the scan mode input the file number that you want to use Load File 1 3 After select the file number input the values for wavelength range scanning step and sampling count You can get better spectrum if you select less scanning step and more sampling count but scan rate is slowed lt Scan Setup gt Start Range 190nm Stop Range 900nm Scanning Step inm Sampling Count 1x After finished the values input select blank sample A and B by using the Aand the Wkey WAVELENTH SCAN Blank Scan WAVELENTH SCAN gt Sample A Scan WAVELENTH SCAN Sample B Not Scanned On the above screen press the START STOP key to start scanning One time pressure to pause and press one more time to re start If you press two times consequently the UV Vis Detector cancel scanning Young Lin UV Vis Detector gt Sample B Scan gt PAUSE After scanning is finished press the ENTER key to display scanning result Press the Aor the Wkey to move wavelength and see absorbance for the wavelength by using the CLEAR SCROLL key If you want to the sample again press the ENTER key or the START STOP key On t
7. Before connecting any new tube or column to the detector flow several mL of clean solvent through the new tube to a waste reservoir This will clean any particulates or oil that may be residing in the tube that could clog the heat exchanger or contaminate the sample cell of the detector 2 3 3 Connections for cable The UV Vis Detector can be controlled by RS 232C and also be controlled and acquired digitally by Autochro 2000 or Autochro 3000 via RS 232C For communication using RS 232C connection use the RX and TX loop connection as shown in the Fig 2 1 At first connect the RS232C cable between PC and UV Vis detector and then RX TX loop connection between each module If you want to use AD module have to connect RS232C cable between PC and AD module for communication And then connect signal and remote cable between UV Vis detector and AD module EJ Imi 1m EE e at m Column Owen TS Ayuboc hiro 2000 CELLS om ie ee Fr Gradient Pump Aulschro Dalia Module Fig 2 1 The RS 232C connection of the UV Vis Detector with the Gradient Pump 2 8 Young Lin UV Vis Detector 2 3 4 The connection of the terminals You can designate input signal recognition of terminals on the Setup Edit mode The UV Vis Detector recognizes the input signal on the point of TTL level 0 5V and Trigger rising and falling edge of the terminals Each terminal function is described as per default values as follows Be careful about
8. EMH Yellow H Blue Black 7H Blue 3 Black amp 44 Green Black 5 Orange 0 N A SODA DNFWNH Fig 5 6 The connection of a transformer BH ANC White S44 Black BH ANC White 34 Black 2 37 Young Lin UV Vis Detector MPU control Main B D The configuration of the pre amp board AMP 442022 4p Trans line used Molex 5559 2p 100mm 204 WG Young Lin UV Vis Detector Ba Yel BVAC 2A singl ais single u ATVAC OSA dual 220 VAC input a 17VAC OSA dual White Ge 18VAC 1A single 1 bla 1 Black 1 i N 15VAC 2A single ed 415VAC 180mA dual ne TISVAC 180mA dual aie eee eres ease eo eee Oe Be el we pe eet as ee ee mean u Whl 40 VAC 1 5A signle White Bla ne 4 i green Case GND input Fig 5 7 The electricity of a transformer 5 3 6 The AC inlet connection Select Switch Noise filter Green 200mm 18AWG brown 100mm 18AWG brown 100mm 18AWG brown 150mm 18AWG Blue 100mm 18AWG Blue 100mm 18AWG White 100mm 18AWG White 100mm 18AWG White 100mm 18AWwG Black 100mm 18 AWG Black 100m m 18 AWG d 1 awe UJIO Fig 5 8 The AC inlet connection 2 38 5 4 Trouble shooting guide Young Lin UV Vis Detector Remaining noise and drift problems can be due to various sources within and external to the system The Table 5 2 provides the check list of
9. Lock Works immediately after selection and the character shaped a key is lightening on off on the upper right of the LCD during locking All keys except the A the Wor the CLR SCR do not work For unlocking press 7 3 0 sequentially 3 8 Execution of Remote mode If you select the remote mode you can use most of the UV Vis Detector s functions The mode should be selected when you control the UV Vis Detector by Autochro 2000 or Autochro 3000 control program On the LCD elapsed time during the remote mode is displayed 3 9 Time event program mode lt TIME EVENT PROGRAM gt Load File Number Clear File No Initial Event No In the time event program mode you can edit file numbers from 1 to 20 which have maximum 40 lines The status worked by a time event program is maintained after the program is finished If you select Yes on Initial event you can set new value for all initial event values If select No previous initial event values are applied lt Initial Ch A gt 1 6 lt lnitial Ch B gt 1 6 Wavelength 254 nm Wavelength 254 nm Sensitivity 0 0001FS Time Constant 9 9sec Offset 0 00 mV Polarity Positive Plot Log Absorbance Sensitivity 0 0001 FS Time Constant 9 9sec Offset 0 00 mV Polarity Positive Plot Log Absorbance 2 20 Young Lin UV Vis Detector lt Initial Sw 1 gt 1 7 lt Initial Sw 2 gt 1 7 Channel Set A Channel Set A Threshold 0 0001AU Thresho
10. Vis Detector 1 Introduction The Young Lin UV Vis Detector is a dual wavelength ultraviolet visible absorbance detector for HPLC The detector is capable of performing applications from capillary to preparative scale making it unmatched by any other detector in its class This manual describes the installation operation troubleshooting maintenance and service for the UV Vis Detector The manual is organized in the following manner Section 1 This section briefly describes the capabilities and specifications of the unit Section 2 This describes the procedure for unpacking and installation of the instrument and for routine operation as an LC detector Section 3 This described operation Section 4 This section describes routine maintenance and service Section 5 This section is a troubleshooting guide Section 6 This section describes the theory of operation 1 1 Functions The UV Vis Detector is the most sensitive and versatile absorbance detector available for HPLC It features dual wavelength capability improved signal to noise performance minimized drift and reduced optical bandwidth The UV Vis Detector is designed to provide higher performance in UV VIS detection for HPLC application With easy programmable front panel keypad and dual wavelength the UV Vis Detector will improve the productivity of any chromatography laboratory Improved Feature and Design e Greatly reduced and compensated RI effect makes to minim
11. and remedy 1 Error and malfunctioning Errors of Vacuum Degasser amp Mixer are occurred when a Not approach normal vacuum state 15 minutes after power on b Vacuum pump operates continuously more than 15 minutes c Rapid pressure drops by solvent leak during normal operation When above errors occurred degasser will alarm with turning red LED and stop automatically Open above 3 6 Young Lin Vacuum Degasser amp Mixer solvent reservoir and check if vacuum lines are installed correctly If you cannot find any thing wrong please contact us or our representative In order to re start turn off and wait for more than 5 seconds and turn on 2 Malfunctioning of 4 way solenoid valve There are a green LED for power and a red LED for valve in the front They display the status If the power LED or any of valves LED is not on check if the pump is on and confirm the connection of BNC cable Refer to the connection drawing of BNC cable If the problem is not solved please contact us or our representative 3 Continuous air bubbling If air bubbles are continuously happened after turning Vacuum Degasser amp Mixer on more than 1 hour check if input and output port and valve fitting are completed connected Do not tighten the screw Fitting and joint part can be damaged If the problem is not solved please contact us or our representative 3 7 Column Oven User Manual Ah YOUNG LIN INSTRUMENT CONTENTS 1 Introd
12. etc There are a power input a power transition switch and a fuse holder on the real panel of gradient pump The fuse specification is 1 A when uses 220V Be sure to be careful on the power volt you use before turning on the pump The Gradient Pump is set at 220V during shipping In case of using 110V change location of power selection switch and use 1 10 Young Lin Gradient Pump 3 2 Mobile phase filter and vessel Solvent vessel should be positioned at higher location than pump and not be positioned below pump and inlet tubing length should be as short as possible This can minimize pressure drop caused at inlet of pump during suction When using solvent having high vapor pressure as hexane formation of air bubble is caused due to large pressure drop in suction part in high flow rate so particular care should be taken and mobile phase should be maintained after air separation filtration and air tightening Mobile phase filter of 10 u m porosity is inserted into inlet tubing in order to prevent entering of small particles Mobile phase filter is blocked if mobile phase is bad or is used for long time it is necessary to clean or change filter in this case 3 3 Preparation of solvent Proper solvent prevents various problems caused during actual analysis Solvent gas removal and filtration are necessary because they have great effect on result of analysis and maintenance of instrument 1 Degassing Solvent gas removal is
13. filter for VIS range enter the used time of a D2 lamp When replace with a new lamp enter 0 enter the used time of a W lamp When replace with a new lamp enter 0 Young Lin UV Vis Detector Lamp Warming set pre warming time for a deuterium lamp CHA Calib set the counter value for voltage correction against 0 0V of channel A terminal CHB Calib set the counter value for voltage correction against 0 0V of channel B terminal Pre AMP Gain set the gain for the pre amp which is connected a light sensor 2 25 Young Lin UV Vis Detector 4 Regular Maintenance and Service 4 1 Light sources The UV Vis Detector uses light sources A deuterium D2 lamp assembly 190 600nm and tungsten W lamp assembly 380 800nm All lamp assemblies are supplied pre aligned and no further alignment adjustment is required 4 1 1 The deuterium lamp The Deuterium Lamp Dz is rated for 1 000 hours of life to 1 2 the original intensity this does not mean that the lamp will burn out after 1 000 hours merely that its intensity will be reduced to 50 of the original output A D lamp using time is counted in the system indicating the total hours of operation To check the lamp intensity Power on the unit if it is not already on Wait for a period of approximately 10 minutes 2 Set the wavelength to 254 nm Select the Reference Light Intensity If the displayed value is equal to or greater than 30 the lamp is good If the d
14. gradient curve and frequency ratio between channel 1 and channel 2 via analog output terminal 10 Setup of DA Voltage Output Offset Display Chart Offset 420 0 1000 Input range 0 9999 Input of offset value for DA voltage output 11 Setup of DA Voltage Output Gain Display Chart Scale 1 00 Input range 0 01 9 99 Input gain value of DA output 12 Change of Zero Point for Pressure Display Pressure Zero No Input range Yes No Changes the zero point of current pressure output As the offset value of a pressure sensor can be largely changed by temperature and pressure recommended to re setup the zero offset after drop pressure 13 Setup of Auto flushing Display Rinse Operate Yes Selection Yes No Select Yes if you want to clean the Gradient Pump inside by an auto flushing pump built in inside It flushes every 3 minutes Young Lin Gradient Pump 4 10 GRADIENT PROGRAM MODE Creates and edits a file s for gradient operation As the mode the Gradient Pump asks you the number of a file and if you want to create or edit a file Can select any file number from 0 to 20 Grad Table No 0 Clear Table No When clears a file flow rate is set to 0 A mixing rate to 100 and other channels mixing rate also set to 0 Other lines except the first line are cleared Open another file during editing mode press the key then returns to the above display When open a file following table are displayed on the LCD s
15. heater off status from temperature program status and turns to initial status from temperature program stop ID TIME SET CV ID TIME SET CV 00 05 20 05 00 05 OFF 05 Temperature program Temperature program stop At this display press the Aor the Wkey to display current temperature and upper temperature limit Act Set Limit 25 20 80 ID TIME SET CV 00 05 20 05 If you want to call another file press the ENTER key The new file does not affect the file which is on use Program Execute File Number 0 4 15 Young Lin Column Oven 3 6 Error Messages The Young Lin Column Oven displays various error messages with beep sound when any errors are occurred There are following operation errors When error message is occurred take measure to remove the error and press the Wkey However any of communication errors are automatically disappeared within few seconds In case error message can not be disappeared refer to 4 maintenance part or contact us Flammable Gas High temp limit Solvent leak Door opened Communication Error gt Command Communication Error gt Value Communication Error gt ID During oven temperature programming if Start or Stop signal is received at rear terminal following message is appeared with beep
16. holder assembly 2 39 Output span of the detector does not match input range of integrator Young Lin UV Vis Detector Press event mark to see if the spike is approximately 20 of scale External triggering device is causing a ground loop problem Use only triggering device with ground isolated from earth ground 3 Baseline drifts excessively Contamination of sample cell windows has occurred Clean cell by flushing with solvents methanol acetone water nitric 5N acid Inspect cell and photodiode for fingerprints and smudges and clean if necessary Solvent from column is changing absorption Column is filled with UV absorbers that are bleeding replace column impure solvent is equilibrating with the column replace solvent with more pure grade switch to a longer wavelength so that background absorption does not fluctuate as much Leakage in the lines from column to flow cell Check lines for leakage Tiny bubbles trapped in the sample cell Increase flow rate and or back pressure Output span of detector does not match input span of recorder or Press Event Mark to see if a spike of 20 of full scale occurs Large temperature fluctuations are occurring Remove detector from the source of hot and cold air Flow cell photodiode assembly or flow cell cover is loose Tighten thumbscrews fastening flow cell holder an
17. ie Keyboard of the UV Vis Detector Press any of following keys for run mode and press the CLEAR SCROLL key and select Yes and press the ENTER then you can select new run mode SINGLE DUAL selects single or dual wavelength If you press the key sequentially selection for single or dual wavelength is displayed accordingly TIME RUN runs wavelength and time constant to be set for the UV Vis Detector according to a time program SCAN scans wavelength range which is set and get absorbance spectrum REMOTE used for control of the UV Vis Detector by external communication Young Lin UV Vis Detector 3 1 2 Keys for edit mode MENU used for change run conditions during run mode PROGRAM used for creating a time program for TIME RUN mode SETUP used for changing setup values for normal status CONFIG used for changing configuration values for the UV Vis Detector lt START STOP gt used for start and stop of a program during program run mode and for scanning during scan mode One press for start and one more press for stop lt Number key 0 9 gt used for input of numbers during edit mode cursor is displayed lt CLR SCR gt used for clear display during edit mode and for changing next selection Also shows next display when a cursor is no there lt A V gt used for moving next display during edit mode or display status 3 1 3 Keys for operation When the UV Vis Detector is on displ
18. is higher than the upper limit at the isocratic the gradient or the slave mode Low pressure limit When current pressure is maintained for 30 seconds lower than the lower limit at the isocratic the gradient or the slave Flow upper limit When flow current rate pressure is maintained for 30 seconds higher than the upper limit at the isocratic or the slave mode Flow lower limit When flow rate is lower than the limit at the isocratic or the Max flow limit When flow rate is higher that the limit at the gradient mode When slave pump is stopped due to protection or When any Communication error errors are occurred during communication for decoding transmission or receipt Max Max frequency Limit Limit When frequency output is over than the range ni limit When pump head is moving unstable due to air or check Remote start When start input is from a terminal or RS 232C Remote stop When stop input is from a terminal or RS 232C Young Lin Gradient Pump 5 Maintenance In the event that problem occurs or it is necessary to change part due to wear of seal in using The Gradient Pump perform maintenance for instrument by referring to the following items 5 1 Caution of use In order to protect instrument take care for the following items in using it 1 After using solvent with sediment such as buffer solution replace solvent with pure water at first and then methanol or iso propanol and make it flow for 30 minutes using each solvent a
19. it with new one at same location and insert it 5 If there are contaminants on surface of plunger remove contaminant by applying methanol on cloth without dust 6 Arrange plunger in the manner that plunger may enter the center hole of loosened pump head assembly and then press pump head by hand so that head may be pressed into body When pressing it press it carefully and take care so that pump head may be maintained vertical 1 39 Young Lin Gradient Pump 7 Tighten head nut with it pressed Tighten it so that left and right sides may be same in turn and tighten it until it may be tightened no more by hand while confirming tightening status finally 5 4 Replacement and cleaning of check valve If check valve is not well operated due to contamination pressure change is severe during operation and pump does not operate properly Many problems of check valve are caused by small impurity which interferes with operation of check valve Therefore if impurity is prevented from entering inside of pump head using mobile phase filter malfunction of check valve is almost not caused Most of problems can be solved by cleaning the check valve cartridge and the pump head inside 1 Separate tubing connected to pump head 2 Loosen inlet and outlet check valve housing of pump head using spanner 3 Wash check valve cartridge in separated check valve housing for about 30 minutes using ultrasonic cleaner with 10 nitric acid solution 4 Usin
20. key Input oven temperature and temperature curve number consecutively then the cursor returned to the ID and adding one line is finished 2 Delete one line When the cursor in on the ID after confirm the ID which is really deleted that you want press the Program key 3 Search a file contents When the cursor is on the ID press the Aor the Wkey to see a file contents ID TIME SET CV 01 19 20 i When you program oven temperature consider the heating and the cooling rate for the Column Oven otherwise it will not work properly The curve types for oven temperature program can be used from 00 to 19 4 13 Young Lin Column Oven and each curve is shown on Fig 7 and Fig 8 The curve No 5 and 15 are linear programming and No 00 is for temperature change at the end of line while No 10 is for temperature change at the start of line Fig 3 1 Various Curves for Temperature Program from 00 to 09 Young Lin Column Oven 3 5 Execution of Temperature Program Press the Start Stop key and input a file number to execute a file for temperature program edited at 3 3 Program Execute File Number 0 After input of the file number following is displayed ID TIME SET CV 00 init 20 05 At this display if you press the Start Stop key consecutively the Column Oven turns to temperature program status from initial status turns to temperature program stop
21. key is used for change the display 1 Setup flow rate Display Flow Rate 000ml min Input rage 000 16 Young Lin Gradient Pump 2 Setup solvent ratio Display A 100 B 0 C 0 Input range 0 100 for all channels The total sum should be 100 if use 3 solvents but can be less than 100 if use 4 channels The difference is output via SW1 terminal 3 Display of current pressure Display Pressure 0 psi or 0 Obar Current pressure is displayed as psi or bar 4 Display of maximum pressure limit Display Hi Limit 6000psi or 420 0 bar Maximum pressure limit is displayed 5 Display of minimum pressure unit Display Low Limit Opsi or 0 0 bar Minimum pressure limit is displayed 6 Terminal switch on off Display S1 Off S2 Off S3 Off Selection On Off S1 is used for display of gradient rate of channel 4 if used 7 Display of accumulated volume and pressure Display Volume Acc 0 0 ul Input range The input of 0 100 0 can be possible when the Gradient Pump is stopped When the unit is set as u input 100 0 then changed to the unit of 0 1 ml The accumulated volume is displayed upto 999 9Lit 8 Elapsed time and input Display Lapse Time 0 0 min Input range 0 999 9 9 Display of relative viscosity Display Viscosity Ratio N A Displays the relative viscosity calculated by reference value and current pressure and flow rate If the viscosity is over range of 0 01 9 9 or the Gradien
22. mark out input the events are executed subsequently If re start the event program all waiting event are neglected Event file number 0 ID Time Event Action Mark Explanation 00 INIT Grad Start N A Starts the gradient mode 01 10 00 SW1 On Wait Outputs a pulse via SW1 when received the mark 02 10 10 SW2 On Wait Outputs a pulse via SW2 when received the mark 03 15 00 Mk out Pulse N A Outputs a puls via mark out 04 20 00 Event 1 N A Changes the event file number to 1 05 20 10 Grad 2 N A Changes the gradient file number to 2 06 100 00 Event Start N A Executes the event table number 01 1 35 Young Lin Gradient Pump Event file number 01 ID Time Event Action Mark Explanation 10 INIT Grad Start N A Starts the gradient program mode 11 100 00 Event Start N A Executes event table number 01 Note 1 When the mark out terminal is connected to the mark in terminal you can switch the SW1 and the SW2 on simultaneously With this function you can operate simultaneous operation for two external valves or other instruments Note 2 However please be careful when use 4 channel valve which is used for the mixing rate ofthe channel 4 They can not be used together 4 12 Error messages and their causes Following messages are occurred by error during use or by external input Table 4 3 describes various error messages and their causes Table 4 3 Various error messages and their cause High pressure limit When current pressure
23. no cursor is occurred As for special reason an operator can change by pressing the CONFIG key just after switching on but ask us for details The configuration can be seen after passing identification by input of password If want to change the password press the CONFIG key and then the CLEAR key and input new password you want The default password is 930 Password After passing identification an operator can input the values for the pump configuration itself in sequence The values already set up are replacements when the SRAM backup fails If change the values they affect the Gradient Pump operation so be sure to clearly understand what they mean 1 Gradient Channel Selection on the Slave Mode Display A Selection A B C When more than 2 Gradient Pumps are used for high pressure gradient operation Each pump should be set up as a master and slaves Following is instructions how to set up each pump Young Lin Gradient Pump SP930D CH1 CH2 A A B C B B A C C C A B 2 Designation of ID for communication Display Instrument ID 1 Input range 1 8 Designate the ID via RS 232C If the received ID is different from the Gradient Pump transmits the communication over TX Also if the protocol is different the Gradient Pump does not handle If the Gradient Pump is set as a slave designate the ID not to overlap for correctly communication 3 Selection of communication parameters Di
24. output Gradient 12V Black line BNC Pump Valve A Red line Cable amp Side Valve B Yellow line CONNECTOR Valve C Green line Side SW1 White line 3 8 Connection of RS232C communication cable The Gradient Pump has communication function so as to perform high pressure gradient by connecting up to 3 sets of the Gradient Pump and is able to perform major function of Gradient Pump using RS232C with computer Command is described in chapter 4 Communication cable is connected by taking RX and TX as chain loop type That is when connecting each pump closed loop is made by connecting TX and RX when connecting it with computer one TX output among each pump is connected to RX pin and RX pin is connected to computer TX pin And in case of using computer Gradient Pump should be in slave mode in case of using Gradient Pump by connecting each other there should be only one master pump More detailed content for this is described in chapter 4 1 16 Young Lin Gradient Pump Master Gradient Pump Slave 2 Gradient Pump Fig 3 3 The connection of RS 232 cable for the Gradient Pump 3 9 Connection of injector and terminal panel START STOP MARK OUT and HOLD of the terminal panel are used for automatic repetitive analysis with the autosampler START operates instrument and perform gradient program and event program together in gradient mode STOP stops instrument MARK IN can control event program during gradient with t
25. sound Remote Start Remote Stop 3 7 Remote Operation via Communication Column Oven can be controlled by Autochro 2000 or Autochro 3000 software In this case Column Oven has to be set remote mode Details are described on the Autochro 2000 or Autochro 3000 software manual Young Lin Column Oven 3 8 Setup Press the OVEN key within 3 seconds within turning on you can edit parameters for initial setup 1 Instrument ID and baud rate Instrument ID 5 RS232C 19200bps The instrument ID can be setup from 0 to 9 for communication Default ID of column oven is 5 Select the baud rate as any of 9600 19200 and 38400bps 2 Safety detection Gas Detect Yes Leak Detect Yes Select the safety function for flammable gas and solvent leak to use 3 Temperature display unit Temperature Unit Celsius Select the temperature unit as one of Celsius or Fahrenheit that you want 4 Input of lower temperature limit Door Open Limit 60 Input lower temperature limit to display error when open the door Means If open the door when Column Oven is ready status and current temperature is over 60 Celsius degree the error message is appeared 4 17 Young Lin Column Oven 5 Setup password for PID constants Reboot No PID Diagnosis Password
26. unscrew four bolts 4 Open the PCB J1 DIP switch for 220V and connect the switch for 110V 3 Select the power selection switch to the voltage that you want to use 5 Close the cover and screw the bolts 4 4 Young Lin Column Oven Jumper f i PCB J1 DIP Switch Selection a AO 20V ov p ke 110V ee 220 o Power Selection Switch SSE ar ololo ol e wW 110 Fig 2 1 Power Selection 2 5 Installation of Manual Sample Injector Column Switching Valve and Columns If the injector or the valve is ordered with column oven they are already installed at our factory However if ordered separately the injector or valve can be field installed Follow instructions as below The columns should be installed by yourself 1 Open the door and unscrew the bolts which are at upper side of the door 2 Detach the valve mount by proper strength 3 Install the injector or valve as per instructions of Rheodyne or Valco 4 Connect the tubes through the white hole holders at each side of the Column Oven The tube length can be 30cm longer than that of required length for convenience 5 Connect the waste line 5 and 6 of the injector to the holes of the waste bottle holder 6 After all connection made assemble the valve mount and tight by screwing the bolts 7 After assemble the valve mount connect the injection tube of the injector to the column and column outlet to the
27. up pump and wait until solvent is completely changed 3 4 Keeping method after use If you do not use Vacuum Degasser amp Mixer more than 1 day use ultra pure water and flow into vacuum degasser and rinse with alcohol completely and keep it Specially when use with buffering solution be sure to rinse Vacuum Degasser amp Mixer otherwise precipitation can be occurred In the case Vacuum Degasser amp Mixer is kept lower than 0 C dry completely after rinse 3 5 Young Lin Vacuum Degasser amp Mixer A Maintenance 4 1 Filtration of solvent Be sure to filter solvent by lt 5 um when specially use buffering solution Keep filtered solvent in the bottle which is cleared from minute particles When use filtered solvent which kept more than 1 week please filter again before use 4 2 Caution of use 1 Do not pressurize the vacuum degasser module More than 120 kPa pressure will damage degassing membrane inside When you put solvent bottles 2 m higher than degasser the pressure is approximately 120 kPa So place the bottles less than 2 m lower 2 Be sure to prevent from any contamination when you connect or disconnect tubes Entering fine dust will reduce degassing efficiency greatly 3 If you want to increase degassing efficiency make parallel connection of input port to output port Do not connect it by serial way 4 Can not use Vacuum Degasser amp Mixer for output of solvent delivery pump 4 3 Diagnosis of malfunction
28. wich Fig 5 1 The circuit diagram of the UV Vis Detector 2 34 Young Lin UV Vis Detector 5 3 2 Configuration of the power board MPU Control D2 lamp electrics Main B D Ignition coil power nput 10YDC 5 DC control ON OFF statio ourrent 300mA check for ignition 450YDC W lamp electrics On Off control Main electrics 5YDC 2A 15 DC 0 5A Main B D 12VDC 1A Fig 5 2 The power board diagram of the UV Vis Detector 2 35 Young Lin UV Vis Detector 5 3 3 Configuration of the main board RS 232C PREISE zn port 2 MPU m M Interface Sa Backup battery 2 phase motor Interface control Interface Power control 17bit Counter m Interface PA with S phase motor 50mS timer control LCD amp Key Interface Interface N b 100mS timer DAC 18bit os gt Channel LCD contrast __ Decoder DAC 12V2 ae a I O Interface Switch Out Self diagnostics uyt YFC _ TTL level I0 Interface Fig 5 3 The configuration of the main board TP2 TP3 TP4 TPS TP7 0000o TP8 TP6 Fig 5 4 The main board circuit diagram 2 36 5 3 4 Configuration of the pre amp board Fig 5 5 5 3 5 The connection of a transformer r 1 oa Molex 5273 Trans line co co co co co co co co co co pa pa he we et alg as FE ed 1 SA4 Black 2 Hal White _ AM Red 34 Black 4 Red wh PONM Black
29. 3 Young Lin Gradient Pump Generally in case it is used at low flow rate or delivery volume should be small one of narrow inner diameter may be used but it is recommended to use proper size because it may be cause to reduction of accuracy of flow rate due to large pressure difference in suction process of solvent in high flow rate On the other hand the standard of 1 is used for connection of the Gradient Pump and injector for analysis and tubing of 0 01 inner diameter is used on the back side of injector The cut surface of tubing should be cut at right angle without dust tube should not be contracted and middle inner diameter shall not be blocked In order to cut stainless steel tubing tubing cutter should be used plastic tubing cutter or shaving cutter should be used for teflon and similar material of tubing and the surface should be clean and have no crumbling Connect tubing of washing port with 1 4 28 fitting as figure 6 inject 20 methanol of 10ml to 20ml into outlet of bottom side using common syringe to make methanol flow out and then make piston cleaned automatically using natural load drop Because cleaning liquid is much consumed due to increase of cleaning liquid flow rate if drop difference is large it is desired to reduce consumption of cleaning liquid by reducing drop difference In case of re using cleaning solution having come out check change period by inspecting base concentration with litmus test paper and
30. A Sensitivity 0 0001 5 FS sets the sensitivity The Channel B output is divided by the sensitivity value Positive Negative Switch 1 Switch 2 1 99 secl 0 99 9 secl 0 99 9 Isecl 2 22 Young Lin UV Vis Detector 3 10 Setup mode The setup mode is used for setting the output of ratio calculation the recognition of terminal input filter type second cut filter autozero and chart marker lt Setup gt 1 14 Ratio Min AU 0 001 Ratio Maximum 5 0 Ratio Minimum 0 1 AZ on Start Yes AZ on WL change Yes CMK on Start No Ext Auto Zero Low Ext Chart Mark Low Ext Start Low Ext Stop Low Ratio Minimum AU enter minimum absorbance for ratio calculation output If this minimum absorbance is not used for calculation the terminal outputs O volt Also if two absorbances are all negative the terminal outputs negative volt and if two absorbances are different in polarity outputs 0 volt Ratio Maximum enter maximum ratio value This value is outputted as maximum volt Ratio Minimum enter minimum ratio value If the ratio is less than the value the terminal outputs 0 volt AZ on Start set autozero when starts a program scan or any run mode AZ on WL Change set autozero when change wavelength CMK on Start set marker output when start a program scan or any run mode Ext Auto Zero set autozero by external terminal input Ext Chart Mark set chart marker input by external terminal input Ex
31. Backup Clear VER1 10 SELF TEST Data Initialize In case no malfunctions or after initialization On initializing VER1 10 SELF TEST OK If you turn on the power of the pump with pressing Config Button the QC set up is on processing and the pump will ask you of maximum flow maximum pressure and maximum motor frequency etc VER1 10 SELF TEST Max Flow 16ml min After input of all these values the calculated pump head volume is displayed for short a while and the pump is operated accordingly Therefore be sure to confirm the pump head volume and ask us if the volume is different from the one that you purchased 1 21 Young Lin Gradient Pump If the pump is used as a master pump and uses RS 232C for gradient Mode the system checks connection for communication with a slave pump Serial Slave Check Pe ae Accepted Serial Link Check Out of Time After initialization is finished the pump is run at isocratic mode If the pump was set up as a slave it will be run as a slave 4 4 CONFIGURATION The values on the configuration are already factory set Understand clearly what each value means The default values should not be changed without special reason On the configuration there are the values for maximum flow rate gradient Mode with two or more pumps and calibration factor for constant flow rate or constant mixing rate operation Meanwhile an operator can not change the values for the pump itself where
32. DA 3 Digital Bessel filter Variable programmable operation for better sample information Young Lin UV Vis Detector Absorbance mode Absorbance range is selectable within 0 001 3 000 AUFS for detection from 190 nm to 900 nm Two wavelength can be monitored simultaneously MaxPlot mode MaxPlot monitors absorbance at two selected wavelengths and plots the maximum absorbance value for each sample components This feature lets you maximize sensitivity even when exact Young Lin UV Vis Detector retention times of specific components unknown within a single spectrum run RatioPlot mode RatioPlot divides absorbance at two selected wavelengths and plots the resulting ratiogram to help detect hidden components that may be present under individual peaks Easy and convenient operation The intuitive front panel display allows for increased with the flexibility you need From the keypad on the front panel you can program such capabilities as dual wavelength operation spectral scanning storage and playback time and event based protocols method storage and retrieval Also accessible from the front panel is the prealigned lamp and cone type flow cell for easy instrument maintenance 1 2 Specifications Wavelength 190 900 nm Lamp 1 000 hr lift Deuterium and Tungsten lamp Spectral Bandwidth 5nm Wavelength Accuracy 1 nm Wavelength Precision 0 1 nm Optics Czerny Turner Mount Blazed Holographic Grating S
33. Oven communication is industry standard RS 232C The data bit is 8 bit the parity is none and the stop bit is 1 bit The connection of the RS 232C cable is shown on Fig 5 and is configured as closed circuit with Gradient Pump and UV Vis Detector Young Lin Column Oven povie g w ac OME Column Oven Fig 2 4 RS 232C Connection with Gradient Pump and UV Vis Detector 4 8 Young Lin Column Oven AUTOCHRO DATA MODULE Windows XP Column Oven COM 2 Re Fig 2 5 RS 232C Connection with Autochro 2000 4 9 Young Lin Column Oven 3 Operation 3 1 Front Panel The front panel is consisted of LCD LEDs for status display and keyboard The LCD is 16 characters and 2 lines and displays as per key operation YOUNGLIN CTS30 First amp Best The LEDs for status display are turning on as follows Power When power is on Ready When the temperature is stable Remote When the Column Oven runs by external control Reset When any of errors occurred The keys are used for followings OVEN Move to mode selection Isothermal Remote Programmed Op and set the temperature Limit PROGRAM Create a file for temperature program A Move up each setting parameter and select displayed parameter v Move down each setting parameter and select displayed parameter Also used for releasing Reset START STOP Start the oven heater and temperature program when the mod
34. Table 3 1 Specially Ratio output can be used for dual wavelength mode and for finding hidden peak over large peak Young Lin UV Vis Detector Table 3 1 Various output setting for Channel A and B BIT Channel A Channel B Max Higher value between A and B AB 3 4 Single and dual wavelength mode Press the SINGLE DUAL key and use the CLEAR SCROLL key until Yes is displayed then press the Enter key to select anyone of Single or Dual SINGLE 4A 254nm Absorbance 0 00001 On the upper line mode channel and wavelength are displayed and using the CLEAR SCROLL key changes the mode from Single to Dual or vice versa On the lower line you can see the following data for selected mode and channel by using the key of Aand V Absorbance absorbance Transmittance transmittance Output output voltage of terminal Sample Energy light energy of sample cell Ref Energy light energy of reference cell Time Constant time constant Sensitivity output sensitivity of terminal full scale Plot output settings such as Max Add Diff Ratio Lamp used time of a deuterium lamp and a tungsten lamp Run Time elapsed time after input of the START STOP key Young Lin UV Vis Detector On the above display press the Menu key and change the values if you want to select wavelength time constant sensitivity output and etc The wavelength for the Single wavelength mode uses the wavelength of the chann
35. The Gradient Pump can perform high pressure gradient by connection with other manufacturer s pump which is able to receive 5V TTL input because setting of frequency output range proportional to flow rate is possible In order to connect it to the product of other company it is necessary to take care in electrical connection Please contact the manufacturer for details In case of performing gradient using the Gradient Pump frequency range of slave proportional flow rate and related designation value are described in detail in chapter 4 3 7 Connection of the Vacuum Degasser amp Mixer The Gradient Pump has terminal board which is able to connect solenoid valve and switch in order to perform low pressure gradient Solenoid valve and switch may be connected for 12V and output is disconnected when on if it is connected with OV The Gradient Pump is configured suitably for three solvents gradient generally but gradient ratio for D solution is output to SW1 terminal board in order to make four solution gradient possible This is 1 15 Young Lin Gradient Pump operated only if sum of gradient ratios of three solutions is less than 100 12V 12V solenoid valve and switch power source VALVE1 gradient ratio A output VALVE2 gradient ratio B output VALVE3 gradient ratio C output OV Valve or switch output grounding 12V 12V solenoid valve and switch power source SW1 gradient ratio D output or switch 1 output sw2 Switch 2 output SW3 Switch 3
36. Used for clear the setting value or scrolling the selection value Also used for clearing the limit for pressure or flow which are already set Also used for change the LCD display of the Gradient Pump 3 A V Used for moving a cursor to display during Isocratic Mode and Constant Pressure Mode and for moving a cursor to editing line during Program Event Mode and Gradient Mode Also used for moving editing line up and down 4 ENTER Used for input of value and parameter scrolled 5 PURGE Used for quick removal and change of solvent in the Gradient Pump Works only when pressing the key during the pump stops while the LCD displays as follows When pressing the key the Gradient Pump runs at high speed so be sure to open the prime purge valve PRIME PURGE Execute 4 e Used for recalling another file number during Gradient Mode and Event Mode Also used for input of a decimal point when input values 1 19 Young Lin Gradient Pump 4 2 Mode Selection There are two modes selectable Editing Mode and Run Mode on the Gradient Pump operation Each Mode is selected by pressing the corresponding operation keys and then Enter key For example if press the GRAD key following message is displayed and the mode is selected by pressing the ENTER PROGRAM GRAD FILE Mode Select Yes If press the CLR SCR key following pressing the GRAD Key following display is appeared and by key pressing the ENTER key then changi
37. WARRANTY Products sold by us unless otherwise specified are warranted for a period of one year from date of shipment or delivery to be free of defects in materials and workmanship If any defects should occur in the product during this period of warranty we will repair or replace the defects parts or product free of charge This warranty shall not apply to defects resulting from following actions 1 Improper or inadequate operation maintenance adjustment or calibration 2 Unauthorized modification or misuse 3 Use of parts that are not supplied by us 4 Disaster 5 Consumable parts such as fuse battery and fittings The warranty period for all parts and repairs supplied under this warranty expires with the warranty period of the original product For inquiries concerning repair service contact your supplier after confirming the model name and serial number of your instrument The contents of this manual are subject to change without notice in accordance with product improvements This operation manual describes the operation over the life of this instrument carefully read this manual to obtain a through understanding of the operation of the unit before attempting to use it Special consideration and precautions for safe and efficient use are also described throughout the manual These appear in the following forms WARNING Warns potentially hazardous situations and outlines the correct procedures or practices required to prev
38. a reference light level less than 30 indicates the lamp to be bad 2 32 Young Lin UV Vis Detector 5 2 Error message If any errors are occurred during self diagnostics or operation following messages are occurred Please fix accordingly Table 5 1 Error messages and its cause and checking CMOS Back up Fail Back up data loss Check RAM battery Check the voltage 3 V VCC 5V DC fail Check VCC TP4 and fuse F3 VDD 15VDC fail Check VDD TP5 and fuse F4 Check VSS TP6 and fuse F5 Grating drive has not Limit switch Check pin connection on J6 Pre amp B D and filter System voltage error Lamp not turn on Check fuse F1 Light path is blocked Check cut filter blocks light path Drive have not work properly Pre amp B D has not Data from pre amp is not Check R and S value on service mode Worked properly normal Filter drive has not Filter drive can not move Ons a Check filter drive movement Worked properly To initial position Flow cell is contaminated Contamination Fat a Check contamination of cell window Or not aligned properly Misaligned cell Optical assemblies are mn Fail to calculate wavelength Check mirror position Misarranged Cut filter check 2 cut filter Check light intensity W lamp ignition Lamp not turn on Check fuse F7 DA AD check Analog Digital conversion I ll 2 33 Young Lin UV Vis Detector 5 3 Configuration diagram 5 3 1 Connection diagram of the UV Vis Detector Oo irki Power
39. adient Pump DETAIL A im DETAIL A IN LINE FILTER ASS Y Fig 5 3 The replacement of the in line filter 5 9 Replacement of pulse damper Diaphragm film of pulse damper may be damaged due to over pressure Blue solution is discharged into outlet of pump in case diaphragm film is damaged so that damper should be replaced In order to replace damper separate power code of instrument and remove cover Because high voltage is maintained within instrument power source of system should be off 1 Power source of system should be certainly off Pull out power code and remove cover 2 Separate tubing from pulse damper using spanner 3 Loosen 4 nuts which fixes pulse damper located at bottom side of the Gradient Pump and remove attached pulse damper 4 Locate new pulse damper and arrange it with 4 holes in bottom side of the Gradient Pump 5 In order to fix pulse damper at proper location tighten 4 nuts at bottom side of pump cabinet Young Lin Gradient Pump mper Diophragm Pulse J Do The replacement of the pulse damper Fig 5 4 1 44 Young Lin Gradient Pump 5 10 Troubleshooting In case general problem occurs as the following table confirm the possible causes regarding this first and then take proper countermeasures The following table is countermeasure in case general problems occur Table 5 1 Problems cause and fixing Feen ee __ __ Am Tubing inside is blocked _ Replace blocked tub
40. aining In order to conditioning it use organic solvent only and do not use buffer solution and base solution Young Lin Gradient Pump 2 Mix iso propanol or methanol by 50 50 and fill instrument with it using the prime purge valve And plug the outlet of pump 3 Set the high pressure limit to 2000psi and make flow rate be 0 2ml min at isocratic mode and do not make air bubble be present inside using prime purge valve again 4 Start pump The pressure will increase upto 2000psi and then the pump will be stopped with a high pressure limit message Repeat this procedure 2 3times and then conditioning of seal is completed Gide Pin A Fump Housing Plunger Fig 5 2 The replacement of the piston plunger 5 3 Replacement of piston plunger If piston plunger is used for long time it should be replaced due to wear The worn piston causes leakage of liquid as well as shortens life of high pressure seal Piston wear is not well observed visually so care should be taken when observing it 1 Loosen tubing of inlet check valve and outlet check valve of pump head 2 Press pump head to main body of instrument by hand and loosen head nut 3 Separate pump head assembly from instrument Then screen and plunger are left in the place where pump head assembly is loosened When loosening it pull it carefully to the direction of pump head guide so that high pressure seal may not be damaged due to eccentricity 4 Pull plunger replace
41. ay status press the following keys to run their functions immediately Contrast adjusts the contrast of LCD display After adjust brightness by the key Aand Y press the Contrast key to return lt LCD Contrast 5 gt LLLLLLILLLLLLLLLLLLLL Auto Zero automatically change current absorbance to zero Young Lin UV Vis Detector Chart Mark outputs 50 chart market of full scale for 0 2 seconds to D A output 3 2 Self diagnostics After turning on the UV Vis Detector checks stored data and power then self wavelength correction The function of self wavelength correction assures accuracy and precision of wavelength When any of fatal errors are occurred operation of the UV Vis Detector is terminated If errors are not fatal the errors will be displayed 3 3 Internal data processing You can get various data output according to setting of Channel A and B Time Constant used for the digital filer If value is larger the baseline is smoother while peak width is wider Sensitivity outputs after dividing by this value 0 1 means 10x Offset outputs after adding this value Polarity outputs by negative or positive Plot outputs after applying various plot calculation gt Calculation between channel A the absorbance of channel A B the absorbance of channel B If A and B are designated as above each setting output for channel A and B is outputted as shown in the
42. cell Assembly 2 31 Young Lin UV Vis Detector 5 Troubleshooting Most problems with HPLC detectors are in fact caused by other parts of the system Noisy and drifting baselines poor reproducibility in quantitative analysis and similar problems are more often the result of dissolved air bubbles contaminated eluents dirty samples or damaged columns rather than of actual problems with detector hardware In order to focus more effectively on troubleshooting detector problems we will first discuss on board diagnostic tips and later present a troubleshooting table organized by symptom cause remedy 5 1 Light intensity diagnostics The detector provides the capability of monitoring relative light intensities at both the sample and reference photodiodes If an unusually noisy baseline is noted relative intensities of reference and sample light should be assessed Acceptable values are dependent upon the flow cell used wavelength of operation and background absorbance The basic guidelines are as follows NOTE This reference light value of 30 is only applicable for wavelengths within the acceptable range for a given lamp 1 Sample light intensity is higher than 50 of reference light intensity if flow cell and lamp are normal condition 2 If sample light intensity is lower than 50 it means a flow cell is contaminated b excessive absorbance by solvent 3 An acceptable ratio of reference to sample light accompanied by
43. creen It is one of examples if want to create a new file please clear Example ID Time Flow A B C CV 00 init 1 00 100 0 0 i 01 0 1 1 10 60 40 0 05 02 12 1 150 40 40 20 06 03 30 5 2 00 30 40 30 06 04 40 2 2 00 20 40 40 06 05 100 0 150 10 40 50 06 06 200 8 2 00 0 40 60 13 07 300 0 1 00 0 30 70 13 08 500 0 1 00 50 50 0 15 09 600 0 1 50 10 40 50 06 10 700 8 2 00 0 40 60 13 11 999 3 1 00 100 0 0 19 Item description ID for two digits The first number is for a file number and the second number is for a edit line number Range is 00 39 Time for gradient time from 0 999 9 in minute Flow for flow rate from 001ml min to the maximum flow rate you setup A B C for mixing rate from 0 to 100 CV for a gradient curve from any of following number you want CV is applied to flow rate and mixing rate as well Range is from 00 to 19 Young Lin Gradient Pump Fig 4 1 Various gradient curve and its number from 00 10 Fig 4 2 Various gradient curve and its number from 11 19 How to create or edit the gradient table Line editing When the cursor is on the ID press the ENTER key to start input of one line and if return the cursor to the ID input of the line is finished If change the operation mode during input the input on the line is not saved nor effected Line adding When the cursor on the time please input any time you want but not overlapped After finished the input the lines are automat
44. ctions 2 1 1 2 Specifications 2 4 2 Installation 2 6 2 1 Unpacking 2 6 2 1 1 What your will need 2 6 2 2 Site requirement of the UV Vis Detector 2 6 2 2 1 Location 2 7 2 3 Connections and control 2 7 2 3 1 Connections for power 2 7 2 3 2 Fluid Connections 2 7 2 3 3 Connections for cable 2 8 2 3 4 The connection of the terminals 2 9 3 Operation 2 11 3 1 Keyboard operation 2 11 3 1 1 Keys for run mode 2 11 3 1 2 Keys for edit mode 2 12 3 1 3 Keys for operation 2 12 3 2 Self diagnostics 2 13 3 3 Internal data processing 2 13 3 4 Single and dual wavelength mode 2 14 3 5 Time program mode 2 15 3 6 Wavelength Scan mode 2 16 3 7 Menu settings 2 17 3 7 1 Setting for channel A B selects the number key of 1 or 2 2 18 3 7 2 Setting for external switch 1 and 2 selects the number key of 3 or 4 2 19 3 7 3 3 7 4 Lamp setting selects the 5 key Key Lock 3 8 Execution of Remote mode 3 9 Time event program mode 3 10 Setup mode 3 11 Configuration mode 4 Routine Maintenance and Service 4 1 Light Sources 4 1 1 4 1 2 4 1 3 4 1 4 4 1 5 The deuterium lamp To remove the D2 lamp The Tungsten lamp W The W lamp To replace the tungsten lamp 4 2 Cleaning of the flow cell 5 Troubleshooting 5 1 Light intensity diagnostics 5 2 Error message 5 3 Configuration diagram 5 3 1 5 3 2 5 3 3 5 3 4 5 3 5 5 3 6 Connection diagram of the UV Vis Detector Configuration o
45. d mobile phase filter and in line filter is 1 41 Young Lin Gradient Pump blocked due to small particles contained in solvent If filter is blocked pressure within flow path of pump is largely reduced when solvent is sucked to generate air bubble make flow rate reduced and make precision reduced so it is desired to check it periodically Main cause of mobile phase filter blocking is growth of bacteria and two causes to block inlet filter is growth of bacteria and use of solvent containing impurity In order to prevent growth of bacteria use at least 10 20 organic solvent or solvent containing growth depressing component If pure water or soluble solution without interfering material is used many bacteria will grow in mobile phase filter though it is replaced with fresh solution everyday Therefore use solvent of HPLC grade filtered well at all times for mobile phase Blocking of in line filter is caused by accumulation of small particles generated due to wear of high pressure seal by using of impure solvent and long use of instrument In case mobile phase filter and in line filter are contaminated condition of filter may be improved by washing it by ultrasonic cleaner with 10 nitric acid solution for 30 minutes If it is not improved by ultrasonic wave cleaning replace it with new filter Change and cleaning of mobile phase filter 1 Separate mobile phase filter from tubing As the surface of teflon tubing of mobile phase filter of i
46. d flow cell cover 2 40 Young Lin UV Vis Detector UV VIS Detector 7301012040 Analytical Flow Cell Assembly 7301012120 Deuterium Lamp Assembly 7301012130 Tungsten Lamp Holder Assembly 1301032120 UV Detector Main Board Assembly 1301032130 UV Detector Power Board Assembly 1301532140 UV Detector Transformer Assembly 1301032470 Pre AMP Board Assembly 1301032160 Connector Board Assembly 1301032170 MPU188 Board Assembly 1301032180 UV Vis Detector Terminal Board Assembly 7301333010 UV Vis Detector Keypad amp Silk 1301533210 LCD Module 20 x 2 2 41 Vacuum Degasser amp Mixer User Manual Ah YOUNG LIN INSTRUMENT CONTENTS 1 Introduction 3 1 1 1 Function and Feature 3 1 1 2 Specification 3 1 2 Installation 3 2 2 1 Unpacking 3 2 2 2 Preparation of installation 3 2 2 2 1 Preparations details 3 2 2 2 2 Site requirement of the Vacuum Degasser amp Mixer 3 2 2 3 Connecting and controlling 3 3 2 3 1 Connecting of the power 3 3 2 3 2 Connecting of BNC cable 3 3 2 3 3 Connecting of tubing 3 4 3 Operation 3 5 3 1 Degassing of the solvent 3 5 3 2 Operating of solvent delivery pump 3 5 3 3 Replacing solvents 3 5 3 4 Keeping method after use 3 5 4 Maintenance 3 6 4 1 Filtration of solvent 3 6 4 2 Caution of use 3 6 4 3 Diagnosis of malfunction and remedy 3 6 Young Lin Vacuum Degasser amp Mixer 1 Introduction It is not easy but imp
47. detector Young Lin Column Oven Waste bottle holder M4x20 Connects the 5 and 6 waste lines to these holes 5 6 waste a A va Sample Loop TA L i a 7 gt Sample Injector g A A Scree these M4x6 om To detector Fig 2 2 Installation of Injector and Column 2 6 Connection of terminals and communication cable The terminals located at rear panel are used for consecutive operation with other instruments such as Gradient Pump and the RS 232C communication is used for control or reading temperature of the Column Oven Terminal input works when short and the terminal output TTL voltage Note When a terminal input is connected with other instrument s terminal input the terminal input may not work In this case confirm other instrument s input circuit so that the terminal input can work Also refer to the terminal I O circuit as follows 4 6 Young Lin Column Oven Input Circuit Output Circuit l Photo coupler Photo coupler Fig 2 3 Circuit Diagram of Terminal I O 1 Terminals and their functions START Used for start of a temperature program STOP Used for stop of a temperature program MARK OUT Start of a temperature program and output a pulse by communication command HOLD Used for output of the Column Oven status to external instrument When the Column Oven is ready output TTL LOW Status to the terminal 2 Connection of the RS 232C cable The Column
48. dule leak rate lt 1x 10 6 cc sec Helium ATM Power requirements AC 220 V 50 60 Hz 25 W 3 1 Young Lin Vacuum Degasser amp Mixer Dimensions 297 x 135 x 495 mm W x H x D 2 Installation 2 1 Unpacking Open the package and carefully take out Young Lin Vacuum Degasser amp Mixer When we deliver Vacuum Degasser amp Mixer it is included the power cord this operation manual and a letter of guarantee Before you install please confirm the contents correctly delivered If not please contact us 2 2 Preparation of installation 2 2 1 Preparation details When install Vacuum Degasser amp Mixer at first please prepare followings to be ready 1 HPLC System Young Lin Gradient Pump HPLC Column Injector 2 3 4 Mobile phase solvent Nut Parrel appropriate fitting types Appropriate wrench types 2 2 2 Site requirement of the Vacuum Degasser amp Mixer The conditions of appropriate installation are as follows 1 Where the temperature is kept 5 35 C and there is no sudden change of temperature 2 3 4 Where there is no generation of corrosive gas Where no generation of moisture much Where no direct and straight sunshine 2 3 Connecting and controlling 3 2 Young Lin Vacuum Degasser amp Mixer 2 3 1 Connecting of the power Check power 220V and its capacity Power consumption is 25W Check fuses mounted above the power cable connector The connector is rear side o
49. e are a power input a voltage selection switch and a fuse holder on the rear panel of the UV Vis Detector The fuse is 0 6A for 220V and 1 2A for 110V Please be careful to note the voltage which is setup at our factory before turning on Normally 220V is setup for exporting detectors 2 3 2 Fluid Connections The Detector fluid inlet is the short tube which comes from the front of the UV Vis Detector As a general rule the less tubing between the column outlet and the flow cell the better Ideally the column outlet should be connected directly to the detector inlet line If this is not possible you should use a minimum length of narrow bore 0 010 inch I D connecting tubing and a zero dead volume union Because different columns use different fittings the detector is supplied with a bare tube end to allow connection to any column accepting 1 16 inch O D tubing You should use nuts and ferrules suitable to your column NOTE Tubing size and position is different for the adjustable pathlength preparative flow cells high pressure microbore flow cell off column capillary flow cell and on column capillary flow cell See their owner s manuals for details 2 7 Young Lin UV Vis Detector Connect the cell outlet to a line leading to an appropriate waste reservoir If bubble formation in the detector cell causes problems you may wish to connect the cell outlet to a restrictor or back pressure device providing 20 60 psi back pressure NOTE
50. e equipped Relatively stable room temperature No straight light such as window side No dusts and vibration Maintain the voltage within 5 variation No high frequency nor strong electro magnetic field Avoid the site from fire spark and flame Be sure to ground Se Yi Bae THe ae FHS YH WH Drain should not be blocked Note Do not install closed to CRT monitor or TV which the Column Oven can affect the display Please place at least 50 wider Note The column oven emits heat at rear part so minimum 10 cm is required between wall 4 3 Young Lin Column Oven 2 3 Power input The column oven is set as 220V at the factory Please be careful to confirm your power voltage If you use 110V please change the voltage for the Column Oven as per 2 4 Power Selection Warning If the setup power voltage is different from your power voltage the Column Oven can be fatal damaged and caused for fire Note Please be sure to ground the Column Oven The tap water pipe can not be used for the earth because the pipe is not made completely of metal Also gas tube should not be used due to safety No proper earth can cause electric shock After confirming the power voltage close the door of the column oven and input the power cable and switch on 2 4 Power Selection If you want to change the voltage from 220V to 110V follow the instructions as below 1 Switch OFF 2 Open and detach the upper cover after
51. e is Program Op ENTER Input of values Young Lin Column Oven 3 2 Self diagnostics After turning on the Column Oven on the LCD initial display is appeared and self diagnostics is executed YOUNGLIN CTS30 First amp Best YOUNGLIN CTS30 Column Oven If no errors during the self diagnostics the Column Oven immediately turns to ready status for oven temperature input by operator or external communication Ver X XX SelfTest If any errors are occurred the Column Oven initialize the data of CMOS RAM and execute the self diagnostics again Ver X XX SelfTest Ver X XX SelfTest CMOS Data Fail Data Initialize Ver X XX SelfTest Ver X XX SelfTest RS232C 19200bps M OK After finished the self diagnostic followings are displayed However if you use the Column Oven as external communication REMOTE is displayed repeatedly Act Set Limit Remote 25 25 80 25 25 80 If press following keys within 3 seconds after switching on the keys operate as follows OVEN Corrects the values for initialization Young Lin Column Oven START STOP Explains key operation shortly ENTER Initializes the data of CMOS RAM for initialization 3 3 Isothermal Operation After above initialization is finished if present mode is
52. e seal The replacement of the piston plunger The replacement of the in line filter The replacement of the pulse damper The drawing of the Gradient Pump carrier housing The drawing of the Gradient Pump IV 1 3 1 5 1 6 1 6 1 13 1 15 1 17 1 18 1 33 1 33 1 38 1 39 1 43 1 44 1 48 1 49 Contents of Table Table 4 1 Various Modes and their operation for the Gradient Pump Table 4 2 Various events and their effects and operations Table 4 3 Various error messages and their cause Table 5 1 Problems cause and fixing 1 20 1 35 1 36 1 45 Young Lin Gradient Pump I Introduction The Younglin Gradient Pump was designed and fabricated as pump for high performance liquid chromatograph requiring exact precise solvent delivery and has very diverse function and excellent performance As the Gradient Pump controlled by microprocessor involves gradient program gradient program of various curves may be executed using the Gradient Pump by connecting 3 instruments or using solenoid valve And the Gradient Pump has frequency output function so it may be connected to similar product of other company which may be operated by receiving frequency input from other instrument The Gradient Pump is a precise small quantity pump which is able to indicate constant pressure operation and elapsed time cumulative liquid delivery quantity relative viscosity etc as well as to realize control of various input and outpu
53. egasser Pull out slowly until when tubes are will with solvent 3 4 Young Lin Vacuum Degasser amp Mixer 3 Operation 3 1 Degassing of the solvent Power on Vacuum Degasser amp Mixer by pressing power switch which is located in the front When you power on check if pump is stopped otherwise stop pump before turning on Vacuum Degasser amp Mixer Vacuum Degasser amp Mixer will start immediately to degas solvent If normally operational green LED located far left is twinkling Time of LED on is getting longer when degassing is proceeding wait for until time LED on is more than 20 seconds Usually it takes 5 20 minutes to degas complete but can be shorter or longer depending on solvents Do initialization until when complete degassing is made 3 2 Operating of solvent delivery pump Set mixing rate constant for all solvents and start pump at flow rate of 1 0 ml min Air bubble will be coming when start To remove the bubble open the prime purge valve and pull out air bubble by syringe slowly until the bubbles are removed After remove close the valve for normal use of pump 3 3 Replacing solvents When necessary to replace solvents during analysis stop pump and replace solvents and re start pump But do not turn off Vacuum Degasser amp Mixer If you want to change solvent filled inside if Vacuum Degasser amp Mixer completely set mixing rate at 100 for the solvent pull out more than 15 ml solvent by syringe or start
54. el A MENU Edit On the Dual wavelength mode you can select two wavelengths for channel A and B simultaneously and use for the absorbance for two wavelengths and calculations between two wavelengths 3 5 Time program mode Press the TIME RUN key and select Yes by using the CLEAR SCROLL key and then press the ENTER key Following is displayed and the Single Dual wavelength mode is executed For execution of the Time program mode load the file number you want to run which is created in the Program edit mode Also you can limit maximum chain count or external trigger by input of the value If you press the key you can re input the value again and edit the file number which is in use However the edited file is applied after a new run Time Event Run Time Event Run Mode select Yes Load File 1 20 Load File Number 11 F ID MD Chain Time Max Chain Count 999 11 OSG 999 Stop File Number 1 20 the number of a program Max Chain Count 1 999 for use of the execution numbers of the trigger START input or the event start On the above display pressure of the CLEAR SCROLL key followings are displayed Then press the Aorthe Wkey to see transmittance light intensity or output data TIMERUN 4A 254nm TIMERUN BP 254nm Absorbance 0 00000 Absorbance 0 00000 2 15 Young Lin UV Vis Detector 3
55. ent from personal injury CAUTION Alert the operator to the correct operating or maintenance procedures required to prevent instrument failure or damage NOTE Provides additional information for operator to obtain the best performance from the instrument Pressurized and hazardous solvents are used in high performance liquid chromatography Take care to follow proper laboratory procedures to insure operator safety Always wear eye skin and clothing protection when operating the instrument especially during sample injection the opening of values etc Gradient Pump User Manual Ah YOUNG LIN INSTRUMENT CONTENTS 1 Introduction 2 Configuration and principle 2 1 2 2 2 3 2 4 2 5 2 6 Configuration Operation method Compensation of compressibility Opening and closing of valve Gradient curve Washing port 3 Installation and preparatory test 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 8 3 9 Inspection and installation Mobile phase filter and vessel Preparation of solvent Connection of tubing Initialization of the Gradient Pump Connection of the frequency in and out terminal cable Connection of the Vacuum Degasser amp Mixer and the switches Connection of RS232C communication cable Connection of injector and terminal panel 3 10 Tubing connection in high pressure gradient 4 Operation 4 1 4 2 4 3 4 4 4 5 4 6 4 7 Key function Mode Selection Starting and Power On Conf
56. f Vacuum Degasser amp Mixer Fuse is 250V and T 0 5A 2 3 2 Connecting of BNC Cable Connect the BNC cable supplied with Vacuum Degasser amp Mixer to the terminal of Gradient Pump as per following drawing Check colors of cable lines Do not make connection wrong Vacuum Degasser amp Mixer Gradient Pump Fig 2 1 The connection drawing of BNC cable 2 3 3 Connecting of tubing 3 3 Young Lin Vacuum Degasser amp Mixer Connecting of mobile phase solvent Vacuum Degasser amp Mixer can connect 4 kinds of solvent If you do not use less than 4 solvents you must plug up the solvent port which is not used Open a dust cover in the front you will see 8 ports 4 ports left are for input and right 4 ports are for output Use a plug screw supplied to plug up un used ports There is solvent reservoir in the upper part of Vacuum Degasser amp Mixer Place solvent bottles on the reservoir and connect input tubes All input tubes have in line 10 um solvent filter in the end to prevent minute particles from entering into pump Connecting of Solvent delivery pump Connect the mixed tube to the input of Gradient Pump The mixed tube is located in right middle of Vacuum Degasser amp Mixer Open prim purge valve by turning in counter clock wise and setting mixing rate as 100 for each solvent and pull out solvent by a syringe Check if all tubes are filled with solvent Do not pull out quickly nor strongly it will damage the in line vacuum d
57. f the power board Configuration of the main board Configuration of the pre amp board The connection of a transformer The AC inlet connection 5 4 Trouble shooting guide 2 19 2 20 2 20 2 20 2 23 2 24 2 26 2 26 2 26 2 26 2 37 2 29 2 29 2 30 2 32 2 32 2 33 2 34 2 34 2 35 2 36 2 37 2 37 2 38 2 39 Fig 1 1 Fig 2 1 Fig 3 1 Fig 4 1 Fig 4 2 Fig 4 3 Fig 4 4 Fig 5 1 Fig 5 2 Fig 5 3 Fig 5 4 Fig 5 5 Fig 5 6 Fig 5 7 Fig 5 8 Table 1 Table 3 CONTENTS OF FIGURE The Young Lin UV Vis Detector The RS 232C connection of the UV Vis Detector with the Gradient Pump Keyboard of the UV Vis Detector The front side of the UV Vis Detector The deuterium lamp assembly The Tungsten lamp assembly The flow cell assembly The circuit diagram of the UV Vis Detector The power board diagram of the UV Vis Detector The configuration of the main board The main board circuit diagram The configuration of the pre amp board The connection of a transformer The electricity of a transformer The AC inlet connection CONTENTS OF TABLE 1 Terminal and their outputs 1 Various output setting for Channel A and B Table 3 2 Programmable events and their works Table 5 1 Error messages and its cause and checking Table 5 2 Various Problems and their cause and remedy 2 5 2 8 2 11 2 28 2 28 2 30 2 31 2 34 2 35 2 36 2 36 2 37 2 37 2 38 2 38 2 10 2 14 2 22 2 33 2 39 Young Lin UV
58. g membrane before use Removal of small particles is necessary to compensate reliable operation of piston seal and is necessary measure for reliability of other components in liquid chromatograph Filtration process is necessary after mixing of solvent and is more necessary in case of buffer to which un dissolved impurities are source of deposits After filtration solvent should be keep in air tight bottle from which small particles are removed once solvent has been filtered it is not necessary to filter this solvent everyday unless reaction produce bacteria or indissoluble material occurs If solvent is kept in storage vessel for more than one week it is desired to filter it again before use 3 Solvent effect on the instrument All parts of the Gradient Pump contacting with mobile phase is manufactured from 316 stainless steel ruby sapphire zirconium or fluorine carbon polymer Most of these materials are sensitive to chloride and it is desired to avoid use of solvent which contains even small quantity of chloride Main solvents which should be avoided especially are as follows Aqua Regia Hydrochloric Acid HCL 20 Bromine HCL 37 Chlorine Anhydrous HCL 50 Copper Chloride HCL 20 Ferric Chloride HCL 75 Ferrous Chloride Hydrofluorsilicic Acid 20 Freon 12 Hydrogen Peroxide Guanidine Lodine Hydrochloride 6M Mercuric Chloride Hydrobromic 20 Dilute Solution In addition it should be avoided to leave chl
59. g pure water rinse check valve cartridge to remove the nitric acid used for cleaning 5 Assemble loosened check valve in the reverse order 5 5 Replacement of low pressure seal Wearing of the low pressure seal is caused when pump has been used for long time without using washing port In order to prevent wear of low pressure seal it is desired to use washing port and it is more desirable in case of using buffer solution Leakage of liquid due to wearing of the low pressure seal is caused between pump head and body 1 Separate pump head with reference to 5 2 2 Separate washer and pull out low pressure seal assembly from pump head body with seal insertion removal tool 3 Replace low pressure seal attached to low pressure seal assembly with new one 4 Assemble pump head in reverse order by referring to 5 2 5 6 Cleaning of flow path within pump In order to prevent occurrence of problem in instrument remove impurity accumulated in instrument and it is better to clean flow path when it is not used for long time Clean inside of flow path in the following method and be careful when treating strong acid and strong base Young Lin Gradient Pump 1 Separate column inlet tubing connected to column 2 Orient column inlet tubing toward waste bottle 3 Set flow rate at 1ml min 4 If injector is installed turn it to injection position 5 Pump 100 iso propanol through pump and injector for 10 minutes 6 Pump distilled water filte
60. h the lamp assembly to the detector Connect the lamp lead to the lower of the two terminals in the lamp compartment CAUTION NEVER loosen the screw holding the lamp to the mount and never attempt to rotate or move the lamp up or down in the mount Doing so will degrade the system performance The lamp is provided as a pre aligned assembly NOTE No harm will be done if the lamp is plugged into the wrong connector but the lamp will fail to light 2 27 Young Lin UV Vis Detector Tungsten lamp Ass y D2 lamp Ass y Flow cell Ass y Fig 4 1 TFig 4 1 The front side of the UV Vis Detector Fig 4 2 The deuterium lamp assembly 2 28 Young Lin UV Vis Detector 4 1 4 The Tungsten lamp W The lifetime of the tungsten lamp is approximately 1 500 hours To check the W lamp intensity Power on the unit and W lamp if it is not already on Wait approximately 10 minutes Set the wavelength to 720nm Select the Reference Light Intensity If the displayed value is equal to or greater than 10nA the lamp is good If the displayed value is between 5 and 10 the lamp is marginal If the displayed value is less than 5 the lamp should be replaced In general a value less than 3 for the 380 450 nm range and less than 5 for 450 800 nm range is indicative of a bad W lamp The W lamp should be used for all wavelengths above 380 nm The user may notice higher relative light intensities for the D2 than the W lamp fr
61. he following display you can see the absorbance and sign to confirm maximum height and size of a peak WL ABS Sign 220 0 00001 gt gt gt gt WL T Output 220 100 0 1000 0 If you want to scan again press the key 3 7 Menu settings The MENU key is used for channels settings lamps on off and variables for external switches CHA setting for the channel A CHB setting for the channel B Switch1 setting for the switch 1 Switch2 setting for the switch 2 Lamp Tungsten or deuterium lamp s on off Key lock Locking keys gt Select Menu 1 6 1 Channel A setting 2 17 1 Channel A setting 2 Channel B setting 3 Switch 1 setting 4 Switch 2 setting 5 Lamp On Off Young Lin UV Vis Detector Press the MENU key and select the number by using the Aor the Wkey and pressure the ENTER key or press the number key that you want to set directly Then you can enter the setting values If you press the MENU key again previous status is displayed 3 7 1 Setting for channel A B selects the number key of 1 or 2 Wavelength Time constant Sensitivity Offset Polarity Plot lt Channel A gt 1 6 Wavelength 254nm Wavelength 254nm Sensitivity 1 0000FS Time Constant 1 0sec Offset 0 0 mV Polarity Positive Plot Absorbance channel A in the single mode enter from 0 1 to 9 9 sec o
62. his input condition MARK OUT can control output with event program during gradient HOLD in case the Gradient Pump is started and prepared to perform analysis HOLD output is disconnected Using this condition waiting status of objective instrument may be induced 3 10 Tubing connection in high pressure gradient In case of performing high pressure gradient using the Gradient Pump it is desired to change tubing connection in order to make solvent mixed well At this time pump outlet used as slave should be connected to tubing to enter damper of inside of instrument in master side using TEE or CROSS 1 17 Young Lin Gradient Pump Injector Column Master Gradient Pump Slave Gradient Pump Fig 3 4 The tube connection of high pressure gradient configuration for the Gradient Pump 1 18 Young Lin Gradient Pump 4 Operation 4 1 Key function The keyboard of the Gradient Pump is consisted of function keys and number keys The function keys are as follows and each function is described 1 START STOP Used for starting or stopping the run of Gradient Pump which is operation on Run Mode On Isocratic or Constant Pressure Mode press 2 times stops the Gradient Pump and press 1 time more starts the Gradient Pump On Gradient Mode press 1 time turns to standby state One more time press starts gradient run of the Gradient Pump and then 2 times press during gradient run stops the Gradient Pump 2 CLR SCL
63. ically sorted by time Please do not input over 40 lines If input by mistake over 40 lines it will not work Young Lin Gradient Pump Line deleting When the cursor on the ID pressure the CLR SCR key However the first line will not be deleted Line moving When the cursor on the ID press the A or the Wkey Opening another file Press the key when the cursor is on the ID 4 11 EVENT PROGRAM MODE At the mode you can edit a event file which can change the gradient mode parameters and the external output parameters for the gradient file that you want to change When you select the mode the Gradient Pump ask you if you want the file number that you want to change and also if you want to clear the file Files can be selected from 0 to 20 The event program file is same with the gradient program file to create edit or save If some of file numbers are being used or run the file s can not be edited Event File No 0 Clear File No Key operation for clear edit create and edit for file and line is same with that of the gradient program mode Press the CLR SCR key to select each item The items for the event program at the mode are described on the Table 4 2 Young Lin Gradient Pump Table 4 2 Various events and their effects and operations Event Operation Init Maintains the first gradient program at initial status of Grad waiting events Start Starts the first gradient program of waiting events aH Stop
64. iguration Isocratic Mode Gradient Mode Constant Pressure Mode 1 1 1 4 1 4 1 7 1 8 1 8 4 8 4 9 4 10 4 11 4 12 Slave Mode Setup Mode Gradient Program Mode Event Program Mode Error Messages and their causes 5 Maintenance 5 1 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 9 5 10 5 11 Caution of use Change of high pressure seal and conditioning Replacement of piston plunger Replacement and cleaning of check valve Replacement of low pressure seal Cleaning of flow path within pump Supply of lubricant Change of mobile phase filter and in line filter Replacement of pulse damper Troubleshooting Number and name of major parts 1 28 1 28 1 32 1 34 1 36 1 37 1 37 1 37 1 39 1 40 1 40 1 40 1 41 1 41 1 43 1 45 1 47 Fig 1 1 Fig 2 1 Fig 2 2 Fig 2 3 Fig 3 1 Fig 3 2 Fig 3 3 Fig 3 4 Fig 4 1 Fig 4 2 Fig 5 1 Fig 5 2 Fig 5 3 Fig 5 4 Fig 5 5 Fig 5 6 Contents Of Figure The Gradient Pump The Gradient Pump Head The drawing of the Gradient Pump rear panel The flow diagram drawing of the Gradient Pump The tube connection of the washing port The connection of frequency I O terminal for the Gradient Pump The connection of RS 232 cable for the Gradient Pump The tube connection of high pressure gradient configuration for the Gradient Pump Various gradient curve and its number from 00 10 Various gradient curve and its number from 11 19 The replacement of the pressur
65. ing by loosening to be from tail side Pressure upper in turn limit is loaded Check valve is blocked Replace outlet check valve Solvent is changed Wait until solvent is completely changed Pressure Change of solvent is Wait until change is completely increases or incomplete performed decreases Wait until pressure is stable Air bubble is in pump head Perform prime purge again Air separation status of Take measures so that air solvent is bad separation condition of solvent Solvent is not may be good and perform flowed out prime purge again Check valve is not good Wash or replace check valve Liquid containing oil flows Remove oil in head sufficiently into head part with strong organic solvent using prime purge port Check valve is bad or Wash or replace check valve defected Air separation or mixing Use mobile phase with well mixed conditions of solvent is solvent and improve air separation bad condition Compression Initialize compensation value or Pressure is compensating reference re input proper value unstable value of configuration mode is wrong Cam shaft is loosened Tighten wrench bolt of cam shaft High pressure seal was Replace high pressure seal worn Pump head was loosened Tighten nut of pump head Young Lin Gradient Pump Liquid is leaked or pressure High pressure seal was worn Replace high pressure seal reduces when i Plunger wa
66. ing neglected is caused actually due to compressibility of mobile phase proportional to pressure and elasticity of high pressure seal so the Gradient Pump is controlled in real time so that occurrence of pulse proportional to pressure may be depressed Control method of the Gradient Pump uses supervision of pressure and control of location simultaneously so it has advantage to improve precision and accuracy of flow rate without being affected by range of pressure and flow rate 2 3 Compensation of compressibility Most of pumps for HPLC analysis are used at high pressure However pulse occurs in high pressure due to compressibility of liquid and elasticity ratio of seal so flow rate is also reduced Occurrence of pulse due to this reduces precision and accuracy of pump flow rate so compensation is necessary for this The Gradient Pump reads actual pressure and calculates compensation value for this compressibility compensating operation to control angular velocity of cam with this value reduces occurrence of pulse flow remarkably as well as improves accuracy of flow rate largely 2 4 Opening and closing of valve The Gradient Pump outputs valve opening and closing signal to terminal board so that gradient analysis may be performed at low pressure as high pressure The Gradient Pump compensates speed of valve circulation opening and closing pursuant to location of cam in low pressure gradient as well as suction interval is broadly distributed
67. ing parts when being shipped Before opening transportation package perform inspection for trace of shock or mistake and if there is abnormality do not open the contents and inform this company of it And if contents are opened perform inspection for existence of shock in the contents and contact with this company when trace of shock is found The Gradient Pump is a delicate instrument so use original box and buffer material as far as possible when re packing it to transport instrument If it is impossible to use original box wrap pump with several layers of buffer material and fill the bottom top and all other sides of pump with buffer material in order to make pump endure shock or vibration during transportation Standard configuration of the Gradient Pump 1 Body of instrument 2 Power source code 3 SUS316 tubing 60cm 4 2 sets of ferrule male nuts 5 Operation manual Site requirement of the Gradient Pump 1 Room with 20 C temperature with variation 5 C with and 60 humidity 2 Where no direct and straight sunlight 3 Plain floor without carpet 4 Where having spare space of 20cm or more 5 Where there are 10 or less voltage change no frequency change and 100 or less grounding point 6 Where there is no generation of corrosive gas and ventilation is well done 7 Where stable power of 110 or 220V is supplied 8 Where not receiving electromagnetic induction from large transformer high frequency heater UPS
68. isothermal followings are displayed Act Set Limit 20 OFF 80 Isothermal Input Operation At this status the heater is not turn on yet If you want to turn on the heater and increase or decrease the temperature press the Start button and select the temperature using Aor the Wkey and the ENTER key then the heater is on Act Set Limit 20 35 80 The upper temperature can be set up to 80 C When oven temperature is over this limit the heater is automatically off and error message is displayed The upper temperature limit is also applied during oven temperature program or remote operation If you want to execute isothermal operation during other operation press the OVEN key Young Lin Column Oven 3 4 Temperature Program The Column Oven can program oven temperature according to time Press the Program key then displayed as follows Program Edit Key Lock No The key lock is used for protection from other user s input If you want to release the key lock please press the keys 4 9 OVEN PROGRAM consecutively File Number 1 Clear File No The temperature program can be edited for file No 0 to 20 while each file is consisted of 41 lines ID TIME SET CV 00 init 20 Edit a temperature program file as follows 1 Add one line When the cursor is on the ID press the ENTER
69. isplay nor 2 Contact us 2 Electric problem normal display 3 Change input voltage or 3 Input voltage wrong Column Oven s voltage Over heat 1 Turn off and after 10 minute turn on Stop and no function Do 3 Fuse out and use Electric problem 2 Replacement of fuse 1 Key locked 1 Release key lock Key input does not work 2 Board problem 2 Contact us Young Lin Column Oven Column Oven 5501032090 Key board ass y 5501012040 Solvent leak sensor 5501012030 Oven fan motor ass y 5501032060 Main Board 1301533210 LCD Module 5501533270 Oven heater coil 5501032100 Terminal board ass y Please contact your local supplier or us when requires service or repair after confirming followings 4 2 3 4 5 6 Model number Serial number Purchase year Detailed problem and its history Cause for problem we zz Hae HS DH WH Any other information available 4 20
70. isplayed value is 1 2 the original intensity the lamp is marginal If the displayed value is less than 1 2 the original intensity the new lamp should be prepared As a general rule the D2 lamp should produce reference light intensity greater than 30 at 254 nm 4 1 2 To remove the Dz lamp 1 Make sure that the power cord is disconnected from the rear panel of the detector 2 Unscrew the screws and remove the lamp assembly on the right front panel 2 26 Young Lin UV Vis Detector CAUTION UV light can damage eyes and skin Always disconnect the power cord before working in the vicinity of the lamp The Ds lamp gets quite hot Care must be taken while handling it to prevent from burning Always allow the lamp to cool before removing it 3 4 _ 3 Disconnect the UV lamp from the detector by gently pulling it straight back toward you DO NOT twist the connector while pulling see lt Figure 4 1 gt and lt Figure 5 1 gt Unscrew the two thumbscrews holding the lamp mount in place and pull the lamp mount straight back towards you Be careful not to lose the two thumbscrews Be careful not to get fingerprints on the lamp 3 To install the Dz lamp Slide the lamp mount onto the lamp housing on the right front panel the mount has a pre drilled hole to accommodate the dowel see lt Figure 4 1 gt and lt Figure 4 2 gt The lamp leads should emerge from the top of the lamp Use the thumbscrews to attac
71. ize noise and interference of solvent peak e Improved baseline stability with heat exchange effect 2 1 Young Lin UV Vis Detector e Minimized drift by heat balance e Easy access to lamp cell as prealigned on the front panel Czerny Turner Mount Split beam Optics e See Through Light Source Cone type flow cell Improved Capabilities Dual wavelength mode simultaneously monitors absorbance at two user selectable wavelength It allows you to get more productive and additional analyte information by using Max Min Add Diff and Ratio Plot modes The UV Vis Detector allows for optimized performance for analytical semi preparative and microbore whether you work in R amp D Analytical research or QA QC 1 Wavelength Range 190 900 nm 1 2 lamps used Halogen and deuterium 2 2 filters used for removal of 2nd light 3 Self wavelength correction 2 Operation Mode 1 Single and Dual Wavelength Mode Enhanced selectivity purity check Calculation Max Add Diff Ratio 2 Programmable Mode Automatic repetition 2 2 20 files and 40 lines Dual wavelength 3 Scanning Mode Blank scan Max Add Diff Ratio Plot between each spectrum 4 Remote Control Mode via RS 232 control and data acquisition 3 External Solenoid Valve and Switch option 1 used for sample preparation 2 recycling for solvent 4 Digitally controlled and operated 1 High speed wavelength scanning 2 High resolution AD
72. ld 0 0001AU Peak Width 10sec Peak Width 10sec Output On Output On Pulse width 1 0 sec Pulse width 1 0 sec Start Delay 0 1 sec Start Delay 0 1 sec Stop Delay 0 1 sec Stop Delay 0 1 sec After finish entering initial event values following displays are appeared Event Action Chart Mark ID Time Select 00 Init Function Mark in N A Edit a time event program as following instructions Add a line enter new time then is displayed on the ID and select After all entering are finished a line is added Lines are arranged in the order of time Delete a line When the cursor is on the ID press the CLR SCR key to delete a line However the first line can not be deleted Edit a line On the above display press the ENTER key to edit a line After finish editing the line press the Enter key to close Confirm a line Use the Aor the Wkey to confirm lines or the CLR SCR to see another display Programmable events and their workings are described in the Table 3 2 When the Mark In is set as Wait the event is waiting for the marker input before works If one more events are waiting for the marker input the events work simultaneously when the marker is inputted 2 21 Young Lin UV Vis Detector Table 3 2 Programmable events and their works Tine Soket event Woks ai Max 0 Function Wait ee as 999 99 min Autozero J o Chart Mark oS Channel
73. ncy Display Max Freq 38400Hz Input range 320 64000 8 Setup of maximum pressure Display Max Pres 9000 Input range 80 16000 for psi unit 9 Setup of frequency and flow rate for external output Display Max Freq Conv CH 1 40000 Hz 20 0 ml min Max Freq Conv CH 2 40000 Hz 20 0 ml min Input range for frequency 320 64000 Flow rate range 0 1 200 If the Gradient Pumps are connected by frequency in and out for high pressure gradient the Gradient Pump outputs frequency in proportion to gradient ratio via DIN output on the rear of the Gradient Pump Input maximum frequency and its corresponding maximum flow rate 10 Setup of origin point at Even Compensation Mode Display DPL 90 DPR 270 Input range DPL lt 82 98 gt DPR lt DPL 176 DPL 184 gt If want to change original point at Even Compensation Mode input desired value However check real pressure change before changing the value as the change affects operation Be careful not to input any value which is not checked or tested DPL is for left head and DPR is for right head of the Gradient Pump Young Lin Gradient Pump 11 Setup of compensation factor intercept and valve position compensation Display CFB 4 CFV 18 Input range 1 Intercept 1 16 2 Correction factor 1 32 The correction factor is for the end point of pressure compensation at 2000psi 140bar and the intercept is for the end point of pressure compensation at 0 psi 0 bar
74. ng mode is cancelled and returned to the first mode PROGRAM GRAD FILE Mode Select No However Run Mode is always effective during Editing Mode All parameter settings work immediately and are not removed after re starting the Gradient Pump by switching on off Table 4 1 Various Modes and their operation for the Gradient Pump ee Gen We _ Bremen Isocratic Mode with constant Input the values for ISOCRATIC ARE CONFIGURATION flow and constant mixing time configuration of the pump i Input of calibration factors for Gradient Mode with gradient GRADIENT display of pressure limit flow and event file rate limit and relative viscosity CONSTANT Maintain constant pressure PROGRAM Edit the gradient file by each PRESSURE inside pump by adjusting flow GRADIENT line Set as slave Mode by Making or editing a file for SYSTEM PROGRAM frequency in and out and gradient Mode condition SLAVE Be EVENT serial communication according to elapsed time Young Lin Gradient Pump 4 3 Starting and Power On On switching on the pump following messages are displayed in sequence and the self diagnostics is YOUNGLIN SP930D Solvent Delivery Pump YOUNGLIN INSTRUMENT If previous values saved at SRAM are not valid or press the Enter key the edited values are initialized done and set up as defaults In case SRAM is in defect VER1 10 SELF TEST CMOS Backup Fail In case the operator press the Enter key VER1 10 SELF TEST CMOS
75. nsertion type is slippery separate it with tubing held avoiding slippage using 1000 sand paper 2 In case of performing ultrasonic wave cleaning wash head part by ultrasonic cleaner with 10 nitric acid solution for 30 minutes wash it again by ultrasonic cleaner with pure water for about 10 minutes then dry it In order to replace filter with new one prepare new mobile phase filter of same size 3 If washing has been completed assemble filter to be replaced newly again Hold teflon tubing using sand paper and insert mobile phase filter of insertion type with center adjusted into middle hole Changing and cleaning of in line filter 1 Separate connected tubing from in line filter assembly using spanner 2 Separate head part of in line filter assembly from body using spanner 3 In case of performing ultrasonic wave cleaning perform ultrasonic wave cleaning to head part for 30 minutes with 10 nitric acid solution perform ultrasonic wave cleaning for 10 minutes with pure water and then dry it In order to replace filter with new one separate filter located at back side of head part 4 Incase of replacing it with new filter replace it with new in line filter located at the location where it was separated and in case ultrasonic wave cleaning has been completed re assemble head part of dried assembly 5 Using spanner tighten head part sufficiently so that there may be no leakage of liquid even at 6000 psi Young Lin Gr
76. nt Pump FACO IN a g o o a g o 9 Q g o a g a a g o a g H 1 WOOEL NO MORI neoan SEAL NO 30 000 Lin oe WARMIMD OISTUNNELT POWER N BEFGFE OPENING LINE VE10 Va SO B0Hr YOUNG LIN INSTRUMENT CO LID WIDE IN KORRES elo AA Fig 2 2 The drawing of the Gradient Pump rear panel PolSo Re TRSHEr icra a m m m m m m a a a a IHLIME Fa FILIEF amp E 4 G x Luana POAT l Onion ss 1 e i FEHEJIVHE 77231 F MJEC OT IL S TEFLON WAM FELE D ae VOLUE IELI Ged COPPA TLA JEEIILE ale bfo Swan Fig 2 3 The flow diagram drawing of the Gradient Pump 1 6 Young Lin Gradient Pump 9 Flow Diagram The flow path diagram of the Gradient Pump is illustrated on FIG 2 3 Teflon tubing is used for inlet check valve of pump head and SUS316 tubing is used thereafter 10 Pulse damper The Gradient Pump uses diaphragm damper which reduces pulse generation of pump and serves as the mixer for solvents in high pressure gradient The Gradient Pump ensures precise flow rate with compression ratio compensating program only so that trouble is not caused for result of analysis without damper in case gradient analysis is unnecessary or in case detector is not sensitive to pulse 11 Pressure sensor This is used to protect instrument from excessive pressure and realize constant pressure drive and compressibility compensating drive by reading pressure between performance
77. nt pressure while the isocratic mode runs by maintaining constant flow rate So input the pressure that you want to operate and upper and lower range of flow rate then current flow rate will be displayed Other factors are same with those of the isocratic mode Young Lin Gradient Pump 1 Input of the pressure that want to operate Display Pres Set 1000psi Input range 0 maximum pressure The Gradient Pump adjust flow rate to maintain the input pressure 2 Display of current flow rate Display Act Flow 1 00ml min Current flow rate is displayed 3 Display of upper limit of flow rate Display Hi Limit 10 0ml min 4 Display of lower limit of flow rate Display Low Limit 0 00ml min 4 8 SLAVE MODE The mode run the Gradient Pump by input of flow rate from the master Gradient Pump via serial or frequency in and out Pressure of the CLR SCI key shows current pressure and flow rate SYSTEM SLAVE Serial Input HPG B If the pump is on the Slave mode it can not be controlled by keyboard Only controlled by master pump or Autochro 2000 or Autochro 3000 software 4 9 SETUP MODE If select the setup mode first you can see the selection of key lock function If select the key lock by pressing Yes shows you the mode which is currently running If the key is locked have to return to the current run mode and can not use key operation further until the key lock is unlocked Press 9 3 0 Key Lock No sequentially f
78. om 380 450 nm However this light represents the second order diffraction spectra of the D2 lamp and should not be used 4 1 5 To replace the tungsten lamp Make sure that the power cord is disconnected from the rear panel of the detector Unscrew the screw and remove the lamp housing the rear housing on the left side of the detector to expose the W lamp Disconnect the W lamp lead from the detector by gently pulling straight back toward you Do not twist the connector while pulling Unscrew the thumbscrews holding the lamp assembly in place and pull the lamp assembly straight back toward you Be careful not to lose the aluminum standoffs or the thumbscrews Be careful not to get fingerprints on the lamp Replace the lamp assembly 2 29 Young Lin UV Vis Detector Fig 4 3 The tungsten lamp assembly WARNING The W lamp gets quite hot Allow enough time to cool before attempting its removal 4 2 Cleaning of the flow cell If at all possible we do not recommend disassembly of the flow cell assembly for routine cleaning purposes Most cells can be adequately cleaned by flushing with several milliliters of appropriate solvent We recommend the following solvents for this purpose 1 Methanol 2 Tetrahydrofuran 3 Methylene Chloride 4 HPLC Grade Water 5 6N Nitric Acid followed by flushing with HPLC Grade Water NOTE Use only spectroscopic grade solvents 2 30 Young Lin UV Vis Detector Fig 4 4 The Flow
79. or unlock Young Lin Gradient Pump If you select the Key Lock to No you can see the setup menu as below 1 Display of upper and lower limit of pressure Display Pressure Limit Low Opsi The maximum value of upper pressure limit should be inputted within the maximum pressure limit of system 6000psi which are already setup and larger by 10 psi or 0 7 bar than the upper limit of pressure The minimum value of lower limit of pressure should be lower by 10 than the upper limit of pressure If input 0 it means no lower limit you apply In case current pressure is higher than maximum upper pressure the Gradient Pump immediately stops so if you want to clear the limit pressure the key CLR SCR 2 Input of upper and lower limit of flow rate Display Flow Rate Limit High 10 0ml min Flow Rate Limit Low 001ml min Input range The upper limit is lower than maximum flow rate and the lower limit is 0 Input of lower limit to 0 means no application of lower limit In case current flow rate is higher than maximum upper limit the Gradient Pump immediately stops so if you want to clear the limit pressure the key CLR SCR Input of 0 means no application of flow rate Young Lin Gradient Pump 3 Input of Viscosity Conversion Value Ref Viscosity 1 00 Test Flow 1 00ml min Ref Viscosity 1 00 Disp Pres 1000 psi Display Input range 0 01 9 99 Reference viscosity can be within 0 01 9 99 Te
80. oroform carbon tetrachloride etc in instrument for long time and use of ammonium hydroxide should be avoided because it has effect on stator and rotor of injector even though it has no effect on pump When not using it for long time keep it with iso propanol filled with in flow path 1 12 Young Lin Gradient Pump 4 Measures when not uses for long time Prepare iso propanol for analysis Open prime purge pump and suck iso propanol of at least 50ml into instrument Separate outlet tubing of pump Press out iso propanol sucked into syringe in prime purge valve and discharge at least 5ml into outlet of in line filter Separate mobile phase filter assembly and block discharge hole and suction hole with cap 3 4 Connection of tubing The following tubing and fitting are used as standard size in flow path of the Gradient Pump fitting size UNF size 1 SUS316 1 16 0 02 10 32 2 Teflon 1 16 1 32 1 4 28 plain bottom 3 Teflon 1 8 1 16 1 4 28 plain bottom O D 1 16 LD 1 32 14 28 FITTING D D 1 810 1 16 1 4 28 FLAT BOTTUM Fig 3 1 The tube connection of the washing port Item 1 is used for tubing from outlet check valve to in line filter and injector item 2 is used for washing port and item 3 is used for tubing connected to mobile phase filter and inlet check valve housing Standard of tubing may be used by changing it with proper one depending on flow rate and pressure used 1 1
81. ortant to select and handle mobile phase solvent in HPLC High Performance Liquid Chromatography Vacuum Degasser amp Mixer is connected a high productive vacuum Degassing module which is built in the mixer and it is equipped with a precise 4 way valve module to provide optimum solvent degassing and mixing to HPLC user 1 1 Function and Feature Degassing of mobile phase solvent Solvent is degassed when passing through the vacuum degasser solvent preparation is greatly simplified and the degassed solvent is ready to use for analysis With an in line vacuum degasser you can realize excellent environment for HPLC analysis Vacuum Degasser amp Mixer consists of four channels for degassing and each channel removes efficiently air and oxygen in the solvent Also solvents which were degassed by each channel were mixed up with 4 way valve and then were degassed one time again Therefore the Vacuum Degasser amp Mixer provides the best optimized solvent The solvent mixing by 4 Way valve module Vacuum Degasser amp Mixer with 4 way solenoid valve can mix up to 4 different solvents easier and more precise than any other mixer available 1 2 Specification Max Flow rate 5 ml min per channel Degassing Channel Tubing Teflon AF Degassing Channel Pressure Rate 70 PSIG testing pressure Vacuum Wetted Materials Teflon AF Peek Class filled PTFE Valve electrical specification DC 12 V 2 5 W 211 mA 570 5 70 F Valve mo
82. performed in order to remove gas such as nitrogen or oxygen contained in mobile phase Contained gas should be removed by air separation before mobile phase is used or while mobile phase is used and the most practical technology for air separation is to insert helium into solvent Helium is easily separated from HPLC solvent so other gases contained in solvent may be easily removed due to diffusion of helium gas Filtration of solvent using vacuum pump is not a effective method than helium separation but it is said to be a helpful method When separating air using helium insert it severely for 10 or 15 minutes before using solvent and maintain proper injection status during use If air is separated from solvent before use gas in the atmosphere is melted into mobile phase within 4 hours Helium purity used should be 99 9 standard laboratory grade When mixing organic solvent such as methanol or acetonitrile into water this mixture contains very small quantity of gas as compared to the quantity of pure composition so it has more strong tendency to discharge gas Back pressure regulator attached to outlet of detector prevents formation of noise in 1 11 Young Lin Gradient Pump base line due to air bubble and mobile phase vessel should be pressurized under 2 3psi pressure with helium if it is desired to reduce gas discharge due to solvent mixing 2 Filtration Solvent should be necessarily filtered through 0 45 u m or less filterin
83. plit beam Measurement Range 0 000 3 000 AUFS Baseline Noise 2 0 x 10 AU at 254 nm Baseline Drift 2 0 x 10 AU at 254 nm Linearity gt 99 7 at 1 0 AU Display 20 lines x 20 characters Flow Cells Type Cone type 2 4 Pathlength Cell volume Cell pressure Flow path material Attenuation Time constant Dimensions Power requirements Analytical 10 mm Narrow bore 5mm Semi prep 2mm 10 ul Analytical lt 1 500 psi Narrow bore lt 1 500 psi Semi prep lt 1 000 psi SUS316 Sapphire PTFE 0 2x 2000x 0 2 9 9 sec 297 x 198 x 495 mm W x Hx D 110 220 10 VAC 50 60 Hz Ane OD UV Vis Detector Fig 1 1 The Young Lin UV Vis Detector 2 5 Young Lin UV Vis Detector Young Lin UV Vis Detector 2 Installation 2 1 Unpacking Carefully unpack the detector from the shipping container and inspect both the unit and packing for any signs of damage If any damage is noted contact the shipping company immediately In addition to this manual the shipping container contains a power cord and any options which you ordered Carefully check the packing list against the contents of the container If anything is missing check the packing materials carefully for the overlooked items If items are missing contact us or your local supplier Place the detector on the bench where it will be used and familiarize yourself with the location and function of the controls and connections 2
84. polarity to connect cables START starts a time program or data acquisition by remote operation with Autochro 2000 STOP stops a time program or data acquisition by remote operation with Autochro 2000 LAMP turns on a lamp when terminal is short When short is released a lamp is turned off MK IN notes marker input during a time program A ZERO executes auto zero of absorbance CHART outputs 50 marker input of full scale for 0 1 second Terminal connections are explained as follows If SW1 and SW2 are connected to GND and TTL is connected to 12V the UV Vis Detector outputs the current maximum upto 0 5A for solenoid valve operation CHA 1 0V outputs 1 0V for full scale 1 0 AU on the channel A CH A 10mV outputs 10mV for full scale 1 0 AU on the channel A CH B1 0V outputs 1 0V for full scale 1 0 AU on the channel B CH B 10mV outputs 10mV for full scale 1 0 AU on the channel B HOLD outputs ready status of the Detector as TTL voltage However If the UV Vis Detector is not ready to receive the START input outputs TTL HIGH MK OUT If press the START key or designate the marker output outputs TT L HIGH for 200ms 2 9 Young Lin UV Vis Detector Table 1 1 Terminals and their outputs ee Te es GND CHBGND e ob Marko o Mozeo oo SW 3 Operation 3 1 Keyboard operation 3 1 1 Keys for run mode Fig 3 1 Young Lin UV Vis Detector TAR e ee e mooo o CEEE TETEE BE
85. prevent contamination of cleaning part by replacing it with very clean new solution 3 5 Initialization of the Gradient Pump When using pump for the first time initialize it through the following process in order to clean flow path and condition high pressure seal This process is necessary in case instrument is installed newly or is not used for long time 1 Preparation of iso propanol for analysis 2 Remove residual air bubble within instrument by turning prime purge valve in counter clockwise and loading iso propanol of at least 50ml with injector 3 Separate pump outlet tubing 4 Press the sucked iso propanol into injector with prime purge valve and discharge more than 5ml to outlet of in line filter 5 Operate pump with instrument outlet open for 2 3 hours at 0 2ml min flow rate and for 1 hour at 1 0ml min flow rate using iso propanol 6 Perform process of 2 using solvent which is desired to be used 1 14 Young Lin Gradient Pump 7 Remove inside residual iso propanol by operating it at 1ml min flow rate for 30 minutes with instrument outlet open 8 Form flow path by connecting injector column and detector tubing mutually Freq in Gradient Pump Freq in Gradient Pump Fig 3 2 The connection of frequency I O terminal for the Gradient Pump 3 6 Connection of the frequency in and out terminal cable The Gradient Pump has frequency input function to be used as slave pump by connecting
86. r requirement AC 110V or 220V 10 50 60Hz Size 297mmW x 198mmH x 495mmD Weight Approximate 27kg Specifications are subject to change without notice for improvement Young Lin Gradient Pump Fig 1 1 1 The Gradient Pump Young Lin Gradient Pump 2 Configuration and principle 2 1 Configuration The Gradient Pump is configured as follows in order to satisfy all functions and performance as a gradient pump for HPLC 1 Keyboard and LCD liquid crystal display This enables all operations of instrument to be performed easily while confirming it through 20 characters x 2 line LCD liquid crystal display using keyboard Detailed method for operation of key is described in chapter 4 2 Prime purge valve Prime purge valve is used for providing new solvent into flow path of pump using common injector When using for the first time or not using for long time insert solvent into pump head using prime purge valve It is possible to change solvent using prime function of keyboard during use of instrument but this function make pump operate in fine speed so it is better to use prime purge valve 3 Mobile phase filter and in line filter Use filter in order to protect system from small particles during operation Mobile phase filter extends life of high pressure seal by blocking small particle entering solvent first and in line filter prevents small particles generated through nor filtration in first stage and wear of seal from en
87. red through pump and injector for 10 minutes 7 Pump 10 nitric acid solution for 5 minutes 8 Wash pump and injector with distilled water filtered for at least 10 minutes 9 Pump 100 iso propanol through pump and injector for 5 minutes Now pump is prepared for use of mobile phase or for the period not being used for short time or long time If pump is not used for long time or there is contamination in flow path due to use of impure solvent it is desired to separate pump head assembly and wash it with ultrasonic cleaner In order to wash pump head separate pump head into parts in the same manner as seal change process of 5 2 wash it with ultrasonic cleaner and assemble each part again At this time the high pressure seal is damaged so replace it with new one 5 7 Supply of lubricant The Gradient Pump necessitates supply of proper lubricant into piston drive part for smooth operation of instrument It is desired to use lubricant or low viscosity grease for piston carrier and pump housing and small amount of grease such as 630 AA for bearing of cam shaft and piston carrier Care should be taken because pumping action is interfered with if lubricant is attached to surface of piston Because shortening of pump life is caused where powder or dust is much generated install instrument where surrounding environment is good 5 8 Change of mobile phase filter and in line filter In case instrument is used for long time or mobile phase is ba
88. ressure seal assembly appears Use seal insertion removal tool to separate low pressure seal assembly Then high pressure seal appears inside of head Young Lin Gradient Pump PUMP HOUSING wa LOW PRESSURE SEAL OUTLET PORT t BACK UP WASHER OSS LOW PRESSURE SEAL HOUSING WASH PORT De es MIDDLE WASHER H HI PRESSURE SEAL O RING amp PUMP HEAD t INLET PORT Fig 5 1 The replacement of the high pressure seal 5 Remove worn seal with seal insertion removal tool and insert new high pressure seal prepared at that place using seal insertion removal tool in the same manner Direction of seal should be such that the direction to see O ring is toward front of head Be careful not to change direction 6 Insert low pressure seal assembly and backup washer 7 Arrange pump head so that plunger left at the place where it is loosened be inserted into center hole of pump head assembly and then press pump head to main body by inserting pump head by hand When pressing it press it carefully so that pump head may be maintained horizontal 8 Tighten head nut in pressed condition Tighten it so that left side and light side may be same and tighten it until it is tightened no more by hand while confirming status of tightening finally 9 Change high pressure seal by applying the process of 1 to 8 to pump head of opposite side Conditioning 1 Prepare organic solvent such as iso propanol or methanol necessary for tr
89. s Pressure sensor of the Gradient Pump is designed as continuous flow path type so as to be suitable for use for HPLC 12 Auto flushing motor It is used for automatic flushing the Gradient Pump inside with 20 methanol for example every 3 minute 2 2 Operation method The Gradient Pump used for liquid chromatograph requires excellent flow rate precision flow rate accuracy and flow rate reproducibility in order to enhance reliability of analysis data and characteristics of detection limit The Gradient Pump minimized generation of pulse by using diaphragm pulse damper and especially designed cam and realizing high speed suction through microprocessor in order to satisfy such requirement Diaphragm pulse damper remarkably reduces pulse generated instantly up to 90 or more from low pressure to high pressure range with charged liquid serving as damper and serves as mixer in gradient analysis Compressive property of diaphragm pulse damper improves flow rate precision by making liquid iso propanol filled inside distribute change of kinetic energy due to flow rate of mobile phase even through compression and expansion processes The quantity of mobile phase contacting with pulse damper is only 1 2ml at 6000psi which ensures that flow path is completely cleared away Young Lin Gradient Pump The Gradient Pump was designed so that integrated flow rate may realize no pulse operation using specially designed cam However pulse incapable of be
90. s the Gradient Pump during running se Starts an event program that is on waiting Event Stops event program s which is waiting after the Stop line However Event Resume is not terminated at the command for recovering event runs already done swt Changes the SW1 terminal output of On Off or Pulse 200m s according to your selection Changes the SW2 terminal output of On Off or Pulse 200m s according to your selection Changes the SW3 terminal output of On Off or Pulse 200m s according to your selection according to your selection Alarm Changes alarms by On Off or Pulse 200mS according to your selection Changes the mark out terminal output of On Off or Pulse 200m s Grad Select a gradient file 0 19 number to run for event program Event Select an event file 0 19 number to run for event program The event program is very useful for repetition run of a gradient or an event program Please refer to following example to create or edit an event program that you want to run An example for the event program After 100 minutes elapse event file No 1 and gradient file No 2 are subsequently executed As for the item Mark it can be N A or Wait If you select Wait the event is started when received the external input such as load of RH7725 manual injector or any other input to designated terminal The waiting events are executed as per external input not by time However the waiting events which need same
91. s worn Replace plunger cleaning port is not used un Fitting in instrument was Tighten fitting in instrument Liquid is leaked u loosened from inside of Damper was damaged Replace instrument Low pressure seal was worn Replace low pressure seal Power voltage is unstable Use stable proper DC power Pump is not or low source operated after Strong induction voltage is Close induction power source in input of power generated in the surroundings surroundings or install instrument away from power source Load is caused to piston carrier Supply lubricant in carrier body Timing belt is loose Reduce clearance above belt by adjusting guide location of timing belt Motor is in defect Inspect connection status of motor Noise is too high cable and replace motor if noise is severe only in high pressure Young Lin Gradient Pump 5 11 Number and name of major parts We at Younglin Instruments have major components of the Gradient Pump on stock for product service 5 years after discontinuance For the Gradient Pump which was sold repair guarantee will be provided at no additional cost in case trouble occurs within 1 year under normal use However the following parts and cases are excluded 1 Consumable components such as ferrule nut filter seal etc 2 Damage to instrument due to default of user 3 Damage to instrument due to natural disaster fire etc
92. splay RS232C 9600 n 8 1 Selection 9600 19200 38400 For RS 232C communication should select the baud rate 9600 or 19200 according to desired rate Date configuration is 8 bit parity is none and stop bit is 1 4 Selection for gradient mode Display Grad Tool Valve4 Selection Serial Freq Valve3 Valve4 If run the Gradient Pump in high pressure gradient by RS 232C select Serial and by frequency in and out select Freq If run the Gradient Pump in low pressure gradient select Valve3 for 3 solvents and Valve4 for 4 solvents Note If you select Valve3 total sum of solvent should be 100 However if you select Valve4 total sum of solvent is not necessary to be 100 and remaining value is output to SW1 output 5 Selection for the Gradient Pump as a slave Display Slave Link Serial Selection Serial Freq Select connection between the Gradient Pumps If the Gradient Pumps are connected via RS 232C select Serial Flow rate and gradient profile are transferred directly to the master Gradient Pump via 1 23 Young Lin Gradient Pump RS 232C If the Gradient Pumps are connected via Frequency in and out select Freq Flow rate is transferred to the master Gradient Pump after conversion of frequency to flow rate 6 Setup of maximum flow rate Display Max Flow 16 0 ml min Input range 001 16 0 If want to change input desired value 7 Setup of maximum freque
93. st flow can be within 001 100 0mL min and Display pressure at Test flow can be setup within 10 9000 psi 0 7 630 bar At Isocratic and Constant Pressure Mode the value will be displayed by following calculation Flow rate at input x Current pressure Relative Viscosity Ratio Viscosity Input X Pressure at input x Current pressure 4 Change of Pressure Unit Display Pressure Unit psi Selection psi bar Select the unit you want to display 5 Selection for Even Compensation Display Even Compensate Yes Selection Yes No If select Yes the Gradient Pump runs by compensated flow rate which is compensated by even compensation value and current pressure 6 Selection for Even Compensation Mode Display Pressure Trace No Selection Yes No Selection of Yes Gradient Pump runs by pressure compensation 7 Input of Value for Flow Rate Calibration Display Flow Calib 1 00 Input range 0 80 1 25 Calibrate the difference between real flow rate and setup flow rate 1 30 Young Lin Gradient Pump 8 Change of Pressure Display Scale Display Pressure Scale 1 00 Input range 0 5 2 0 In case pressure displayed is not same with real pressure input the difference value for calibration 9 Selection of DA Voltage Output Display Chart out Pressure Selection Pres A B C Flow Visco Curve SL CH1 SL CH2 Output of pressure mixing rate flow rate viscosity
94. symptoms possible causes and some remedies Table 5 2 Problem CAUSE Various Problems and theirs cause and remedy REMEDY 1 Spikes on baseline Bubbles passing through cell Degas solvent and or supply back pressure to the sample cell also check all high pressure fittings for leaks both liquid and gasses External triggering device is creating electrical noise Check electrical lines for good connection and or interference from broad cast radiation Check for ground loops Extremely large supply voltage transient on the AC line Remove systems that consume high power from the AC line 2 Noisy baseline random Sample cell windows are contaminated Flush cell with solvents methanol acetone water nitric 6N acid water and check for leaks Sample input line has a leak Check all lines from the output of the column to the input ofthe sample cell for leaks Bubble trapped in the sample cell Increase flow rate and or back pressure on cell Recorder or integrator is grounded and is causing a ground loop problem Check recorder with voltmeter to see if either of the signal inputs is grounded to case or earth ground Photodiode window is dirty or not held down properly to the cell holder Remove and clean photodiode window Sample cell is not screwed down to the main unit Check sample cell mounts and cell
95. t 1 0ml min flow rate 2 Do not use solvent to corrode stainless steel material which is less than pH 2 3 3 Do not install instrument where corrosive gas is generated or where there is carpet on floor 4 Do not change flow rate rapidly in order to prevent from wrong operation of instrument damage to column and damage to damper 5 Do not operate instrument with excessive force 5 2 Change of high pressure seal and conditioning If instrument is used for long time high pressure seal is worn out to produce leakage of solution In this case after replacing it with new seal it is necessary to condition it in order to make seal be used for long time at high pressure Change and condition high pressure seal in the following method In case instrument is used for the first time after purchasing it is desired to perform training and it is better to change all seals of both head when change seal due to long use Leaked liquid flows out through washing port Change of high pressure seal 1 Loosen tubing of inlet check valve and outlet check valve of pump head 2 Press pump head to main body of instrument with hand and loosen head nut 3 Separate pump head assembly from instrument At this time screen and plunger are left at the place where pump head assembly was loosened When loosening it pull it carefully in pump head guide direction and take care not to damage plunger 4 If backup washer in back side of pump head is pulled out low p
96. t Pump is stopped N A is displayed Young Lin Gradient Pump 4 6 GRADIENT MODE The Gradient Mode is used for running gradient operation of the Gradient Pump The gradient is run as per a gradient file and an event program file If select this mode to run the Gradient Pump ask you of followings 1 Input of the gradient file number to run Display Grad File No 0 Input range 0 19 2 Input of repetition START number for a gradient file Display Max Chain No Input range 1 999 3 Input of the event file number to run Display Event File No 0 Input range 0 19 After input all of above values the Gradient Pump runs for gradient operation At the gradient mode key and its operation is as follows Press the CLR SCR one of following displays will be displayed Display 1 Display 2 Display 3 ID Time Time A Sere F TimeSi S2 S3 01 01 1 00 100 20 obh Ot 9 on soft on If press the key open a new gradient file and an event program file to edit and also set up the repetition run number Consecutive pressure of the START STOP key work as follows At Standby status one press of the key changes to initial status At Initial status one press of the key changes to run status for gradient At Run status one press of the key warning is appeared for Now Gradient Mode and 2 consecutive pressures of the key changes to initial status 4 7 CONSTANT PRESSURE MODE The mode runs the Gradient Pump by maintaining consta
97. t Start set start when low or high point by external terminal input Ext Stop set stop when low or high point by external terminal input Ext Lamp Off set lamps on off by external terminal input Filter Type set the type of a digital filter Average 1st RC or Bessel Second Cut Filter set use with the second cut filter 2 23 3 11 Configuration mode CONFIGURATION Password Young Lin UV Vis Detector Input password to enter the configuration mode by pressure of the CONFIG key The default password is set as UV Vis Detector at the factory The password can be changed by pressure of the CLR SCR key If you forgot the password ask us or your supplier lt Config gt 1 12 Instrument ID 1 COM1 Set 9600 n 8 1 D2 Lamp Time 0 WLamp Time 0 2nd Filter WL 220nm 2nd Filter2 WL 220nm Maximum WL 1100nm Minimum WL 190nm CH A Calib 0 CH B Calib 0 Lamp Warming 20sec Pre AMP Gain 10 Instrument ID set the ID used for communication between instruments Same ID can not be used ID of detector is 1 COM1 Set sets COM1 for communication between UV Vis Detector no parity 0 data bit 1 stop bit D2 Lamp Time W Lamp Time 2nd Filter1 WL 2nd Filter2 WL Max Wave set maximum wavelength Can enter up to 1100nm Min Wave set minimum wavelength Can enter down to 190nm 2 24 set the wavelength of the 2nd filter for UV range set the wavelength of the 2nd
98. t and program execution with involved event program and RS232C communication This enables execution of operation connected with the autosampler remote operation using computer etc The Gradient Pump is equipped with cam and damper devised especially to exert high level liquid delivery performance as well as function and it has compression ratio compensating function high speed operation to improve precision and accuracy of flow rate simultaneously On the other hand it has washing port of same type as head in pump head for durability of instrument and uses high density fluoro resin seal so that life of seal is largely extended even in solvent using buffer It has in line filter at outlet to prevent small particles from entering into column in order to protect column and is able to set upper and lower limit of pressure and flow rate In addition the Gradient Pump has a function of prime purge and key locking for convenience of user Major features of the Gradient Pump Gradient Solvent Delivery Pump 1 Wide flow rate range and excellent flow rate linearity 2 Realization of ultra precise liquid delivery by compression ratio compensating operation 3 Selection of 16 types of gradient curves of low pressure and high pressure by involvement of gradient program 4 Execution status control pursuant to proceeding of time with involved event program 5 Constant pressure and constant flow selection operation 6 Indication of elapse time accum
99. tering column and shortening life of column 4 Terminal board This is used to connect surrounding equipment such as solenoid valve switch autosampler etc for constant pressure gradient 5 Pump head assembly Pump head is a device to deliver solvent through check valve and reciprocation of piston which is composed of check valve washing port and high pressure seal washing port and low pressure seal etc Pump head assembly is equipped with washing port which may be used efficiently for using buffer solution Young Lin Gradient Pump 6 Main circuit panel The panel is equipped with electronic circuit part related to microprocessor control to realize diverse functions of the Gradient Pump 7 Motor operation and power circuit panel It is equipped with stepping motor control circuit and power circuit to drive motor Stepping motor control circuit drivers motor by micro step so it is possible to obtain constant motor speed even in lower noise and lower flow rate than common control circuit Fig 2 1 The Gradient Pump Head 8 Driving mechanism This is a device to change kinetic energy generated from step motor into reciprocation motion and transfer it to pump head This device uses especially designed cam so that occurrence of pulse in flow rate may be minimized even through single head pump and is composed of stepping motor for driving piston carrier carrier housing cam location sensor etc Young Lin Gradie
100. uction 4 1 2 Unpacking and Installation 4 3 2 1 Unpacking 4 3 2 2 Installations 4 3 2 3 Power input 4 4 2 4 Power Selection 4 4 2 5 Installation of Manual sample injector column switching valve and Columns 4 5 2 6 Connection of Terminals and Communication Cable 4 6 3 Operation 4 10 3 1 Front Panel 4 10 3 2 Self diagnostics 4 11 3 3 Isothermal Operation 4 12 3 4 Temperature Program 4 13 3 5 Execution of Temperature Program 4 15 3 6 Error Messages 4 16 3 7 Remote Operation via Communication 4 16 3 8 Setup 4 17 A Maintenance 4 19 Contents of Figure Fig 1 1 The Younglin Column Oven Fig 2 1 Power Selection Fig 2 2 Installation of Injector and Column Fig 2 3 Circuit Diagram of Terminal I O Fig 2 4 RS 232C Connection with Gradient and UV Vis Detector Fig 2 5 RS 232C Connection with Autochro 2000 Fig 3 1 Various Curves for Temperature Program from 00 to 09 Fig 3 2 Various Curves for Temperature Program from 10 to 19 Contents of Table Table 1 1 The specification of the Column Oven Table 4 1 Error messages and their Cause Table 4 2 Problems and their fixing 4 1 4 5 4 6 4 7 4 8 4 9 4 14 4 14 4 19 4 19 Young Lin Column Oven 1 Introduction Young Lin Column oven is excellent oven used for HPLC to maintain and program column temperature The Column Oven has various functions and self protection Also it uses PID to control temperature and uses an air forced heat exchange technology to enhance temperat
101. ulated flow and relative viscosity 7 Integrated configuration and convenient use Young Lin Gradient Pump Specifications of the Gradient Pump Gradient Pump Solvent delivery dual plunger pulse compensating operation Solvent delivery mode isocratic constant pressure gradient and external input Head volume cleaning part integrated 64 u Lx 2 Flow rate range 0 001 16 0ml min Flow rate accuracy 2 at 1 0ml min Maximum pressure 6000psi 0 420 bar Flushing Automatic every 3 minute Solvent compressibility compensation high speed even compensating operation type Material of liquid contacting part SUS316 zirconium sapphire ruby UHMWPE Gradient table 10 step 10 file at least 999 9 minutes per 1 file 16types of curves and linearity Number of gradient channel 3 channels frequency is used 4 channels Vacuum Degasser amp Mixer used Valve operation method phase compensating random circulation method Solvent mixing ratio 0 100 program execution capability 0 01 Event table at least 999 9 minutes per 1 file for 10 step 10 file change of gradient event execution status connected outside switch operation waiting operation alarm program External control input frequency input instrument stop and start marker for event execution control RS232C communication External output RS232C frequency output 2 channel pressure value and gradient curve relative viscosity 1 0 VFS and 10mVFS Powe
102. ure precision With RTD temperature sensor used for control it provides accurate temperature control in wide range of temperature Following table describes the standard specification of the column oven Fig 1 1 The Young Lin Column Oven 4 1 Young Lin Column Oven Table 1 1 The specification of the Column Oven Fon OOOO Maximum temperature leak sensor Over temperature block Safety Elammable gas sensor Installable option Manual injector Column switching valve Damper Mixer LCD display Temperature Setup value Error 16 character x 2 lines RS 232C protocol for temperature control protocol External input Start Stop External output Hold Mark Out Voltage 110 220V 50 60Hz 3A Dimension 210mm W x550mm H x 400mm D 4 2 Young Lin Column Oven 2 Unpacking and Installation 2 1 Unpacking Unpack the box and pull out the column oven carefully Confirm the serial number between that of rear panel and that of final inspection test sheet Also confirm the voltage stated on the rear panel carefully if it is matched with one that you use The Column Oven is set as 220V at our factory Also confirm the packing list if all accessories are included in the box If any of them are missing or defective please contact local supplier or Younglin Instrument 2 2 Installation As HPLC uses large amount of solvents which can damage or be volatile when leak please be careful 4 2 3 4 5 6 7 8 Vent utility should b
103. utputs analog voltage by converting the set values to maximum scale after divided by the set value sets offset for analog output sets polarity for analog output sets for analog output select the wavelength for each channel The channel B uses the wavelength for the Young Lin UV Vis Detector 3 7 2 Setting for external switch 1 and 2 selects the number key of 3 or 4 lt Switch 1 gt 1 7 Channel Set A Threshold 0 0001 Abs Peak Width 10 sec Output On Pulse Width 1 00sec Start Delay 0 1sec Stop Delay 9 9sec Channel Set sets the channel for the external output switch Threshold enters the threshold for the switch working by absorbance variance Peak Width sets the second for the switch working time Output sets the switch status on or off when absorbance variance is over the threshold Pulse Width sets the pulse width when the switch working is set as pulse Start Delay sets the second for start delay time of the switch working when absorbance variance is over the threshold Stop Delay sets the second for stop delay time of the switch working when absorbance variance is less the threshold 3 7 3 Lamp setting selects the 5 key Turns a lamp on or off A deuterium lamp is on 3 seconds after set to ON for warming up lt Lamp control gt 1 2 Deuterium Lamp On Halogen Lamp On Deuterium Lamp On Off Halogen Lamp On Off Young Lin UV Vis Detector 3 7 4 Key

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