Triart plus
Contents
1. YMC USER S MANUAL Column Care and Use Instructions Triart plus Triart C18 plus Triart C18 ExRS plus Trart C8 plus Triart Phenyl plus Triart PFP plus 1 Introduction Thank you for purchasing a YMC high performance liquid chromatography HPLC column Triart plus is a multipurpose reversed phase column using hybrid silica gel Triart plus has excellent resolution and high durability as well as conventional Triart products In addition a new hardware and packing technology of Triart plus column offer increased maximum operating pressure up to 45 MPa Triart plus column which are manufactured under highly controlled conditions must pass a series of strict tests before being accepted for shipment Please refer to the column inspection report To ensure optimal performance and durability of the column please read these instructions carefully before using this column 2 Specifications Particle Usable temperature range Packing material size i Functional group Usable pH range betas Upper limit um recommended e FL pH1 7 70 C Triart C18 ExRS ExRS 1 0 12 0 pH 7 12 50 C a eee 20 40 C Triart Triat Phenyl 2 1 10 100 10 0 aie TriartPFP TriartPFP Pentafluoropheny pf ee 1 0 8 0 3 Recommendations for column connections detector settings and data processing considerations The PTH at the end of the product code indicates the style of column endfittings see below for details Consideration of co2. e are shown in the specifications table in section 2 In addition please note the following Column lifetime varies depending on conditions of use such as pH temperature and mobile phase composition In general usage at higher temperatures higher concentrations of buffer salts additives and lower concentrations of organic solvents can shorten the column lifetime When using the column under alkaline conditions for a long time a column should be used with a low concentration about 1 10 mM of organic buffer such as triethylamine or glycine at a lower temperature less than 30 C The mobile phase containing methanol is expected to give a longer column lifetime under alkaline conditions than other organic solvents will Make sure of the miscibility among the organic solvents and take care to prevent the precipitation of buffer salts additives to avoid overpressuring the column When possible the sample should be dissolved in a solvent that is of the same composition as the initial mobile phase Using a stronger solvent than the initial mobile phase for sample dissolution might result in distorted peak symmetry and degraded resolution To prevent exposure of the column to excessive pressure the mobile phase and sample solution should be filtered through a 0 2 um membrane or smaller to remove particulates 6 Column cleaning general method After using mobile phase not containing buffer salts additives Flush the column with solutio
3. n containing a higher ratio of organic solvent for washing out the compounds that have a great capacity for retention in the column Usable concentration of organic solvent is up to 100 A cleaning solution containing THF might be effective when removing highly hydrophobic lipid soluble substances that are adsorbed onto the gel After using mobile phase containing buffer salts additives First replace with a water organic solution containing no buffer salts or additives A ratio of water to organic solvent should be set at the same proportions as a mobile phase Then flush the column in accordance with the method described above General proposals Flushing with 100 water after using the column around the pH limit might shorten column lifetime Flush the column with water organic solution as described above Once macromolecules such as proteins or polysacchandes are adsorbed onto the gel they are hardly removed even if solvents with high eluting capability are used To avoid contamination of the column by them conduct sample pretreatment carefully before introduction into the column Alternatively use a guard column YMC CO LTD YMC Karasuma Gojo Bldg 284 Daigo cho Karasuma Nishiiru Gojo dori Shimogyo ku Kyoto 600 8106 Japan TEL 81 75 342 4515 FAX 81 75 342 4550 URL www ymc co jp E Mail sales ymc co jp UM141119AE 2 2
4. nnector and endfittings Jen teva e Port depth EE of endfittings Tubing and connector Column product code p g i PH ca 2mm 0 09 inch Parker Parkerstye gt lt a Port depth Tubing must have flat ends and must bottom out in the column endfitting Tubing must be connected to the column correctly to avoid creating a void between the column frit and tubing which can cause a leak and result in poor column performance e g peak tailing loss of theoretical plate number The extra column volume has a great impact on band spreading To minimize the influence of band spreading on chromatographic performance especially when using columns in 2 0 mm I D and smaller the LC system should be optimized as described below gt The shortest possible length of tubing with narrow inner diameters tubing less than 0 15 mm 0 006 inch I D is recommended should be used for the connection from the injector to the column and from the column to the detector Make sure not to have a void in the connection gt Use a detector equipped with low volume flow cell designed for the narrow bore column gt Use an injector for the narrow bore column and a low volume sample loop A sampling rate and a detector response time constant should be optimized to acquire more than 10 data points across a peak UM141119AE 1 2 YMC USER S MANUAL 4 Shipping solvent 100 Acetonitrile Replace with this solvent for storage When replacing a mobile
5. phase containing buffer salts additives extra care must be taken to prevent salt precipitation 5 Precautions for use The correct direction of the solvent flow is indicated by an arrow on the column identification label Do not disconnect a column from the LC system before the pressure drops to zero The column pressure limit and recommended flow rate are the following Usable pressure range Column D and recommended flow rate Particle size Regular use Upper limit Mobile phase condition acetonitrile aqueous solution recommended 2 1mm ll D 0 2 mL min 3 um 5 um ee 45 MPa 30mmID 0 4mLmin 4 6 mm I D 1 0 mL min Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life Adjust the flow rate appropriately because the pressure changes depending on the column length temperature types of organic solvent etc Aqueous or non aqueous solvents can be used as a mobile phase Repetitive replacement among solvents with large difference in polarities might degrade the column performance In general acetonitrile methanol and tetrahydrofuran THF are recommended for regular use Please also see the recommendation above under alkaline mobile phase conditions When using THF as a mobile phase be mindful of the solvent resistance of your system or tubing PEEK parts are especially unsuitable for use with THF Recommendations of pH and temperature for column us
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