ValveLink Manual6 3-00
Contents
1. Applications Lure lock valves and syringes Pressure upgrade PerfusionPencil manifold System Ser ara sees esaeeovicnsetoe 5 Perfusion systems Micro manifold installation Pressure Kit Multi barrel Perfusion Pencil Chromatography Gel Blot washing PEEN EE EE OE ee 11 Manual control 3 way valves Back panel Case and valve grounding IVC O e o E 13 Serial COM mMMAMICAMOUNG sepen PP ino ni isere 16 TEQUES E E lo E eee ree 18 Dead volume Small bore Teflon tubing Backflow Syringe reservoirs AutoPrime System Faulty valves Leaks Replacing valve fittings Hardware troubleshooting oale ty ASAC A e AEEA aS 23 WA ica 27 Josef Kewekordes II Heath Lukatch Dave Ilstrup Gabriel Stern y 1996 2000 AutoMate Scientific Inc All rights reserved Introduction FOR RESEARCH USE ONLY The ValveLink is designed for solution switching use in research ap plications ONLY AutoMate Scientific Inc cannot be responsible for injury or death resulting from medical or pharmacological use Hardware Overview The ValveLink 8 and 16 are simple digital manual valve controllers designed for use with research automation software Valves can be controlled by digital TTL signals from any I O card or device Eight pushbuttons allow manual user control with eight bi colored LED indicators 16 LED s for ValveLink16 s plus a bank selector switch described later A serial RS 232 port is provided for fur ther valve co2. number is needed as long as you include a note with the following 1 Your name company institution name phone number and an address to return the repair to 2 Your original invoice number and date if available 3 A sentence stating the nature of the problem and any steps you have already taken to fix or identify it Does the valve energize click De energize Open close Leak 4 Whether the valve was driven by any device other than an AutoMate controller and its operating and holding voltages 5 The application used with the valve along with the exact chemical s and the duty cycle pulse rate if used 6 AStatement of Chemical Exposure as follows a These valves have not been exposed to chemicals other than H O except as described above b All applicable Safety and Handling data sheets for the above described chemicals are enclosed c We accept full 22 responsibility for any injury to AutoMate employees or employees of the valves original manufacturer caused by handling of the residues of chemicals contained in or on the valves to be returned 7 An authorized signature title and date AutoMate will gladly fax you a form with easy blanks to enter the above information We will process your return and notify you of further information as quickly as possible Leaks amp Replacing valve fittings The second bane of all liquid delivery apparatus These hints may help 1 Make sure the valve is turned off as o
3. these openings must not be blocked or covered Do not use this product on a bed sofa rug or other similar surfaces This product should never be placed near or over a radiator or heat register This product should not be placed in a built in installation unless proper ventilation is provided 4 Never push objects of any kind into the product through the cabinet open ings as they may touch dangerous voltage points or short out parts that could result in fire or electric shock Never spill liquids of any kind in the product 5 This product should only be connected to the AC power source indicated on your product system s information label If you are not sure of the type of AC power available consult your dealer or local power company Only connect this product to a power outlet that matches the power requirements of this product 6 Do not allow anything to rest on the power cord Do not locate this product where people will walk on the cord 7 If you have to use an extension cord with this product make sure that the total amperage rating of all equipment plugged into it does not exceed the amperage rating of the extension cord Also make sure that the total of all products plugged into the main AC power outlet does not exceed 15 Amps 8 Unplug your product from the main electrical power before cleaning Do not use liquid cleaner or aerosol cleaners Use a damp cloth for cleaning 9 Do not use this product near water 29 1
4. turns them off 2 Low bank Binary Addressing mode Inputs 1 3 are decoded as a binary number if the fourth TTL Input is low Turns off mode 1 3 High bank Addressing mode Inputs 1 3 are decoded as a bi nary number if the fourth TTL Input is high Turns off mode 1 4 Preselect mode TTL Input 8 turns the preselected valve on or off Requires a valve number parameter Turns off mode 1 5 Master mode Requires a valve number parameter 6 One at a Time mode The baud rate Mode 8 may not be changed via the serial com mands for obvious reasons See pages 13 15 for a complete description of these modes 17 Techniques Dead volume Dead volume should be minimized in order to switch solu tions as rapidly as possible Dead volume for this purpose refers to the volume in the single piece of tubing between the manifold and final destination perfusion chamber 96 well plate etc A certain amount of time must pass for a new liquid to clear the previous one from this final piece of tubing Solution switching takes place at the manifold assuming each channel s tubing has been primed from its valve to this point Therefore the manifold should be positioned as close to the ultimate destination as possible while the valves can be some distance away The micro manifolds available from Auto Mate Scientific are designed to be inserted without tubing directly into orifices in some perfusion chambers This absolutely min
5. feature is useful if a control solution is required when ever all other solutions are stopped 5 One at a time Mode When active Mode 5 automatically closes all other valves whenever a valve is opened either by push button TTL input or serial input This guarantees that no more than one valve is open at a time The only exception to this mode is an All Open command through the serial port code 192 7 4800 Baud The ValveLink normally expects serial communic ations at 9600 baud When Mode 7 is active the ValveLink listens for serial codes at a slower 4800 baud rate While several modes decode the TTL inputs the push buttons and serial input will always toggle valves according to which modes are active Remember button 0 leaves mode setting If Modes 3 and 4 are both active the user will be prompted for the Mode 3 pre selected valve first then the Mode 4 master valve 14 Multiple modes As mentioned above most of the six modes above can be active concurrently such as Mode 1 Addressing Mode 4 Master Valve and Mode 5 One at a time Valves are opened or closed accord ingly Only Modes 1 and 2 cannot both be on simultaneously Example mode selection LED s red off green on 0 1 2 3 4 5 6 7 Blink Off On Off On On Off On 0 blinking mode select press button 0 to leave 2 on Addressing Mode valves 8 15 2nd ValveLink8 4 on Master Valve 5 on One at a time Mode 7 on 4800 baud seria
6. 0 Unplug this product from the main power outlet and call for service under any of the following conditions A If the power cord or plug is damaged or frayed B If liquid has been spilled into the product C If the product has been exposed to rain or water D If the product has been dropped or the cabinet has been damaged E If the product exhibits a distinct change in performance indicating a need for service STOP If you ever have to remove the main system unit cover observe the following precautions A The power supply cord must be unplugged before the main system unit cover is removed Separe le cordon d alimantation et puis enleve le couvercle B Once removed the cover must be replaced and screwed in position before the power supply is plugged back in Apres le couvercle en place et remettre le cordon d alimentation Battery Warning Statement CAUTION There is a danger of explosion if the battery is replaced incorrectly Replace only with the same or equipment type recommended by the manufacturer Discard used batteries according to the manufacturer s instructions See inside of front cover for battery specifications ATTENTION Il y a danger d explosion s il y a remplacement incorrect de la batterie Rem placer uniquement avec une batterie de meme type ou d un type recommande par le constructeur Mettre au rebutled batteries usagees conformement aux instructions du fablicant See inside of front co
7. AutoMate Scientific Inc 336 Baden Street San Francisco CA 94131 U S A 800 998 MATE 6283 P international 415 239 6080 fax 415 239 6801 A e mail info autom8 com www http www autom8 com ValveLink 8 16 ValveLink 8 AutoMate Scientific Inc User s Manual Version 2 2 y AN AutoMate Scientific Inc ValveLink8 Part 01 18 For indoor use only from 5 to 40 C Do not get wet or subject to visible condensation Clean with a mild soap solution with a damp cloth only This equipment must be earth grounded Use any of the screws on the bottom of the case See page 12 A Do not unplug valves while on Do not attempt to repeatedly cycle valves on and off in under 10 milliseconds If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Supply voltage ValveLink8 controller 12 V AC up to 2 4 Amps 50 60 Hz External power supplies USA amp Japan 110 V AC up to 0 26 Amps 60 Hz European 220 V AC up to 0 17 Amps 50 Hz Internal battery type 3V Lithium coin battery type BR2325 coin or CR2032 23mm x 165mAh Please replace fuse with standard 2 5 Amp M medium time blow fuse CE ValveLink 8 amp 16 ValveLink 8 LES 00600009 User s Manual Version 2 2 The ValveLink is intended for research use only Table of Contents MTC apparel peo eee ee eee re 2 Hardware overview
8. asing the Reset button LED 0 should begin flashing Indi vidual modes are toggled on and off by pressing their button while LED 0 is flashing The LED associated with each button should change from red to green with green representing ON All of the modes can be used simultaneously except numbers 1 and 2 which cannot both be on Press button 0 to return to normal ValveLink operation with any modes activated The ValveLink cannot remember which modes are active when powered off Therefore all modes are deactivated when the ValveLink is turned off or unplugged However modes are main tained after pressing the Reset button Hold button 0 down while resetting the ValveLink to view and change the current mode set tings as described above NOTE Reset also closes all valves Mode Descriptions 1 Binary Demultiplexed Addressing Mode Valves 0 7 for two ValveLink8 s TTL inputs 0 2 decode into a binary number 0 7 representing which valve to open When this 3 bit number changes the previous valve is closed When Mode 1 or 2 is used TTL inputs 0 3 are used for addressing and no longer directly control valves 0 3 Example TTL input 0000 0101 opens valve 5 its decimal equiv alent For ValveLink16 s additional bit 3 is used as described in the next paragraph to further select bank 2 2 Addressing Mode Valves 8 15 2nd ValveLink8 This is the same binary decoding of bit 0 2 as Mode 1 but bit 3 is used to select between two Val
9. d flow regulator Always calibrate flow rates regulator values are relative see below Leave tubing between reservoirs and valves long enough to raise and lower reservoirs 1 Assemble ringstand 2 Attach valve unit 3 Attach reservoir bracket s 4 Insert reservoirs 5 Attach provided tubing to upper valve barb cut as desired but see note above first 6 Connect tubing between bottom valve barbs and manifold 7 Connect flow regulator tubing to manifold outflow and your perfusion chamber downsizing if necessary 8 Plug numbered valve cables into ValveLink ports Calibrate flow rates by running one line into a graduated cylinder for a fixed period of time 5 Standard Teflon Perfusion System without pressure Green clamps close to mani fold vacuum trap Unused lines should be replaced with plugs on manifold or temporarily clamped off just above manifold to avoid back bleeding into empty lines Priming one line at a time reduces bubbles Connect your vacuum trap line directly to the outflow of the manifold for run ning cleaning solutions and drying your lines quickly Determine dead volume from the manifold to sample by measuring time and volume required to flush a colored liquid with a clear one Flow rate adjusted by relative height of reservoirs and included flow regulator Always calibrate flow rates regulator values are relative see below Lea
10. end of the day fill each reservoir with cleaning solution distilled water or appropriate liquid and press Start A protocol can be easily written to flush each valve in sequence Saline solutions allowed to dry in stainless steel valves will cause them to rust This is considered improper use We also recommend rinsing your valves once before their first use Beware of flaking Teflon tape or silicone fitting glue Large debris are often the cause of valve failure Try backflushing a valve that is suspected to have failed for this reason by connecting a light ly pressurized liquid to the outflow of the valve while it is energized Sometimes this ejects the clog No Draino 2 Do not operate AutoMate valves with homemade controllers delivering over 12V DC or attempt to cycle them rapidly lt 10ms 3 Do not exceed the rated operating pressure gt 100psi Before returning any valves to us please check 1 That it was purchased in the last 12 months AutoMate and valve manufacturers record each by serial number 20 2 That the valve is faulty and not the controller channel find a valve that works in a particular output channel and try the bad valve in that port to see that it still does not function 3 If itis leaking see 1 above and the section below If it qualifies for all three of the above AutoMate will accept the valve for subsequent return to the factory Repair or replace ment is at their discretion as AutoMate does n
11. he top of Valve enclosure shown without front plate the pre po Ec 7 a couple of inches find the BAA ee offending cable trace it back 1 S5 from the bad valve and 3 Plastic split loom cable sheath 21 3 carefully slice its heat shrink tubing longitudinally Heat to remove it The wires may have come apart or shrink broken here inside the next piece of electrical tape ae isolating the two leads otherwise within the cable or valve itself which can be further determined with the volt ohmmeter If you find the break resolder the con nection wrap the inner wires in separate pieces of electrical tape retest the valve and reassemble the sheath following the above instructions in reverse order Valve returns amp Replacing a Valve If you need to return a defective valve you may either re turn the entire valve assembly for repair evaluation or remove and return the individual valve from its case This only involves five screws remove the two outer plate screws holding all of the valves into the black metal case the single screw holding the plas tic sheath into its underside hole the electrical tape at the top and bottom of the sheath the offending valve s cable from the plug end up through the valve casing and finally the two screws holding the valve on to the front plate Please package the valve s carefully and ship freight prepaid to AutoMate s address on the back cover of this manual No RMA
12. if it is used for high viscosity fluids flushing after each use is recommended Cutting The Removable Tips are shipped with 1 5 3 8 cm length polyimide needles They are intended to be cut to a desired length by rolling a razor blade on them against a hard surface However this may leave a small crack or barb on the tip To get a flat cut score the coating of the needle with a ceramic cleaving stone or a diamond cutter and pull directly apart making sure not to pull at an angle You may notice a larger outer tube enclosing the lower part of our smaller 100um needles This is simply for added rigidity and can be cut if needed Chromatography ANMA Fraction collector or waste Gel Blot Washing System Without a pump flow rate is determined by relative height of reservoirs 1 Position reservoirs above column ringstand 2 Attach valve unit to col umn stand 3 Run tubing from upper valve barbs into reser VOIrs 4 Connect short pieces of tubingbetweenlowervalve barbs and manifold 5 Connect manifold outflow to your pump with 1 16 i d tubing 6 Pump outflow connects to column inlet as usual 7 Plug numbered valve cables Flow rate is determined by relative height of reservoirs 1 Position reservoirs above washing area 2 Attach valve unit to ring stand 3 Run tubing from upper valve barbs to reservoir nipples 4 Connect tube from lower valve barbs to
13. ill escape through the tubing leaving the stale solution with a ruined pH and gas concentration The 8 channel AutoPrime System uses eight valves per four liquids with the second set used as waste 19 flush valves to waste Shortly before a solution is to be switched on its flush valve opens and re primes the upper tubing with fresh solution from the reservoir down to its delivery valve where a tee fitting is located connected to the flush valve The flush valve is then closed and the delivery valve can be opened The previous solution in a delivery sequence can continue to flow while re prim ing occurs Faulty valves The bane of all liquid delivery apparatus Despite AutoMate s best efforts and pursuit of high quality valve manufacturers some valves fail How often have space shuttle launches been delayed due to valve problems We can only pass along our valve supplier s one year warranty with their disclaimer Improper use or mishan dling of the units in the opinion of the manufacturer voids this warranty their exclamation mark too In order to minimize valve problems please observe these guidelines 1 Clean your valves after each use Accumulated debris or precipitate will rapidly cause leaking valves and seals Use an in line filter if you expect a chance of particles in your solutions Put your AutoMate controller s programmability to good use by writ ing a washing protocol At the
14. imizes dead volume resulting in switching times of under a second AutoMate Perfusion Systems include a blue disposable flow regulator which will increase dead volume if used improperly The regulator can either be eliminated if controlling flow rate is not necessary or positioned before the manifold to eliminate dead vol ume However this second method only controls the flow rate of individual solutions instead of all solutions when located after the manifold Therefore the researcher can either limit the flow of a single liquid or purchase flow regulators for each line A final method for reducing dead volume is to use smaller bore tubing although this may also reduce flow rate resulting in the same switching time as for larger tubing Small bore Teflon tubing AutoMate Scientific s micro manifolds use 1 16 o d tube ports which can either be used with this size tubing or enlarged up to 1 16 i d for valve hose barb fittings Please inquire about Au toMate s Teflon tubing and nut and ferrule valve fittings for small bore use Also see the micro manifold instructions on page 7 Cutting Teflon tubing at an angle makes it easier to insert 18 Backflow When a manifold is used in a plumbing arrangement none of its ports can be left unconnected or liquid will simply flow back wards out of the opening Small green hose clamps are included with AutoMate Perfusion System drippers which can be easily at tached to short
15. ip xxx 100 250 or 360 um 04 xxx specify 100 250 or 360 um Epoxy or silicon glue may be needed to completely seal connections between valve tubing and the manifold tip in ports Tighten removable tip for minimum dead volume One of the eight lines may be connected to vacuum through a valve programmed to suck the dead volume clear between solutions Chemical Information The tubing inside the manifold body is polyimide nylon The largest connection tubing is vinyl The Removable tips include a medical grade polypropylene Luer lock fitting with a fused silica quartz needle coated with polyimide These materials are resistant to most acids bases and organic solvents To avoid dust contamination we recommend pre rinsing the Perfusion Pencil and Tip with distilled water Also it is good practice to discard the first few microliters of solution before using the device The Perfusion Pencil and Removable Tips are shipped non sterile They can be chemically sterilized or autoclaved However repeated autoclaving may weaken the adhesive bond between the Lure lock fitting and the needle Maintenance You can expect several years of useful lifetime for your tip if you wash it daily Use a syringe or vacuum to pull first water then alcohol three times each through the tip If the Multibarrel Perfusion Pencil is filled with pure salt solution leaving it in the syringe overnight will not usually cause the tip to block However
16. ircuit board in clips labeled Spare 29 EPROM Installation Instructions 1 Open the ValveLink case as described in the preceeding paragraph Remove the last EPROM slowly with a flat screwdriver or IC 2003 AutoMate Scientific remover noting the dimple orientation p ValveLink 1 3__ pin 1 on its left side Insert the new chip with the same orientation being careful not to bend any pins 2 Make sure that the ValveLink turns on then turn it back off before reinstalling the case top Save the old EPROM until you are completely satisfied with the new one EPROM s can be erased and recycled and they cost around 3 99 each So if you re happy with an upgrade it s worth the few pennies stamp cost for U S customers to drop them in an envelope back to AutoMate International or busy U S customers will be excused if they cannot return old useless eproms 24 Safety Instructions The following instructions pertain to the risk of fire electric shock or bodily injury Please read all of these instructions carefully 1 Follow all the instructions and warnings marked on this product or included in this manual 2 Do not use this product on an unstable cart stand or table This product may fall causing serious damage to the product 3 Slots and openings in the cabinet and the back are provided for ventilation To ensure the reliable operation of your product and to protect it from overheating
17. l diameter pipette delivery The package can be added to any new or existing per fusion rig from AutoMate any third party manufac turer or even homemade Connect to house air tank or compressor 30 to 100 psi Precision regulator delivers zero to 10 psi For Single cell or Whole cell Superfusion Multi barrel Perfusion Pencil 8 micro bore tubes into 1 outflow e Single cell and patch delivery e Rapid solution change e Micro liter dead volume e 250 amp 100 micron removable tips System Set up Diagrams Perfusion System vacuum trap Unused lines should be replaced with plugs on manifold or temporarily clamped off just above manifold to avoid back bleeding into empty lines Priming one line at a time reduces bubbles Connect your vacuum trap line directly to the outflow of the manifold for run ning cleaning solutions and drying your lines quickly Determine dead volume from the manifold to sample by measuring time and volume required to flush a colored liquid with a clear one Instructions on this page for Econ omy perfusion systems Lure lock fittings Pressurized systems or Pinch valve systems Regular hose barb Teflon perfusion systems please see the next page Lure lock systems should also see photo on page 3 Find additional photos and diagrams of the pressure system on pages 4 and 8 Flow rate adjusted by relative height of reservoirs and include
18. l rate In this example when button 0 is pressed to leave mode selec tion all LEDs will flash waiting for the master valve Mode 4 to be selected Instant mode select In order to ease mode selection especially when the ValveLink is frequently turned on and off individual modes can be selected when power is turned on Just as button 0 is held to enter mode selection described earlier instead simply hold down the button corresponding to the mode to be activated during power on or reset It is not necessary to press anything to leave or finish instant mode selection Only one mode can be activated in this manner at a time However button 6 activates the frequently used combination of Modes 4 amp 5 master valve and one at a time As described under Mode Selection Modes 3 4 and 7 each require specific valves to be designated for their function The user will be prompted with all LED s flashing to select a pre desig nated or master valve after using instant selection for any of these modes 15 Serial Communications All of AutoMate s controllers are capable of communicating with a host computer connected to a chain of several devices Serial communications must be set to either 9600 or 4800 baud see Mode 8 and must be sent over a standard null modem cable Usually this is anormal serial cable with a null modem adapter Null modem cable diagram 1 ground 3 ie AutoMate has implemen
19. mplied war ranty or resulting from the use failure or malfunction of any product including damage to property and to the fullest extent permitted by law damages for personal injury even if AutoMate Scientific Inc has been advised of the possibility of such damages or if this warranty is found to fail its essential purpose AutoMate Scientific Inc s liability is limited to the reimbursement of the cost of the product All other warranties including but not limited to warranties for fitness or merchantability for a particular purpose are expressly excluded No verbal changes to this policy will be authorized AutoMate Scientific Inc manufacturers and sells goods in accordance with U S federal state and local laws but assumes no responsibility or liability for laws regulations duties or taxes imposed by any foreign country All terms and conditions hereof shall be governed by the laws of the State of California 27
20. ntrol by serial devices All three input sources digital serial and push buttons are simultaneously active and the LED s constantly display the current valve status The ValveLink is designed to drive 12 volt DC solenoid valves plugged into RCA jacks on the back of the box Why not connect valves directly to your computer Several reasons 1 Most computer interfaces provide 5V signals not strong enough to drive most valves 6 to 12V DC and up 2 Computer interface signals are often too noisy for electro physiology amplification The ValveLink is designed with special low noise circuitry to minimize interference with high gain amplification 3 Finally many solenoid valves are designed to be opened with a short full voltage pulse then held in with a lower voltage to keep the valves from heating The ValveLink au tomatically provides dual voltage hold in TTL stands for Transistor Transistor Logic a 5 0 volt standard for representing on and off used by the ValveLink for computer valve control 2 ValveLink Laboratory Applications Perfusion A ValveLink can be programmed to precisely switch solutions perfusing over biological samples for physiological research The ValveLink s circuitry is designed to open valves with low noise for electrophysiological amplification Several features Modes have been built into the ValveLink for advanced perfusion use Washing Combined with an AutoMate ported wa
21. ot have the ability to open the valves to determine the cause of trouble We may be able to loan replacement valves which may be billed if the valve factory finds abuse on the user s part and refuses to honor the warranty Stuck pinch Valves Occasionally pinch valves will stick closed Usually this is a sign that they are about to completely fail but sometimes they can be rejuvinated by simply prying them open First energize turn on the valve then using a flat screwdriver gently force the white plunger open where it normally pinches the tube closed The valve may begin functioning normally Also pinch valves will not usu ally work without a piece of pinch tubing installed Valve Cables One problem that is easy to diagnose and fix is a broken cable connection Use any ohmmeter to measure the resistance across the inner pin and outer barrel of the RCA plug end of the valve cable while unplugged from the controller A resistance of zero ohms indicates a short or infinite resistance means one of the wires is broken in the cable assembly This occurs most often at the joint between the heavy black cable and the valve lead wires under the heat shrink tubing inside the outer black sheath a few inches from the valve itself This can often be reached without completely dis assembling the valve enclosure Simply 1 remove the screw inside the black valve box holding the plastic sheath in its hole 2 remove the black tape at t
22. pieces of tubing to act as plugs for unused holes Likewise one must be careful not to open any valve whose reservoir is completely empty or inflow tube is disconnected Or dinary solution pressure in other lines will force liquid back up the outflow tubing through the valve and either back into the reservoir or out the disconnected inflow onto the floor Again clamp off any unused lines at the manifold or be sure all valves are closed while changing connections Syringe reservoirs AutoMate offers 30ml syringes photo on page 3 as alterna tives to its 100ml polypropylene reservoir cups Connected directly into luer lock fittings in the valves this method eliminates wasted solution in tubing between the reservoirs and valves Small 2 way stopcocks are included when this option is ordered These are placed between the syringe and valve fitting in case syringes must be disconnected before completely empty Simply loosen the luer lock from the valve fitting and remove the syringe and stopcock to gether These stopcocks are also useful if there is a chance of a valve opening after its reservoir is empty see above paragraph AutoPrime System The AutoPrime Perfusion System is de signed to deliver oxygenated or other gas sat urated solutions without administering stale liquid which has remained stationary in the tubing long enough to lose its oxygenation Unless one uses hard walled i e Tef lon tubing gas w
23. ports 3 way single solenoid valves 2 wire Program the ValveLink as you normally would for 2 way valves after determining which of two inlets or outlets are opened with the on and off states 11 Back Panel Serial Address 12 V AC Power up to 2 4 Amps 50 60 Hz pg 16 A AA A uoaa TERA Serial 1 2 3 4 5 6 7 Digital Input Address SS MaS E 12v oc Valves E E ValveLing 8 Par 6 01 18 For Research Use Only Mado in Caldomia USA ji 4 Use undors de ca o acre ler Groumcing Serial RS 232 Ports Digital inputs In computer and Out see previous page next device in chain 12 V DC valves See page 16 110 or 220 V AC Power Case amp Valve Grounding This equipment must be earth grounded Use any screws on the bottom of the ValveLink case for grounding Order AutoMate part no 01 05 Low noise valve and case grounding package for an extra grounding wire attached to each individual valve ex tending back to the controller Each of this item grounds four 4 valves Please order two for eight channel perfusion systems This item must be ordered at the same time as the valves Connect the ground wire from the valves to a screw on the bottom of the control ler case 12 Modes The ValveLink includes several useful features as auxiliary Modes The user alters the current mode settings by holding but ton 0 down while powering the ValveLink on or while pressing and rele
24. pposed to leaking 2 See all of the guidelines in the previous Faulty Valves section especially 1 3 Identify whether the valve leaks constantly under pressure inside or outside Try backflushing if an internal leak 4 Check and replace obvious loose or worn tubing 5 Outside leaks usually occur at the fittings and can be stopped with a bit of silicone glue epoxy super glue Cyanoac rylate or Teflon tape around the threads of the dry fitting screwed back into the valve Be sure not to use too much glue or tape as they can easily cause clogs or leaks of their own Overtightening can also cause the plastic fittings to leak hand tighten Glue on valve fittings is intended as a gap filler for small open ings between the threads and hole not as an adhesive Few glues will adhere to Teflon Hardware troubleshooting Should the ValveLink LED s fail to light while power is turned on check your power connections source and on off switch Dis connect all valves to check for a short Check the back panel fuse on ValveLink16 s Finally if all else fails unplug the ValveLink and check the fuse on the internal circuit board following these instruc tions ValveLink8 remove all of the screws on the bottom and 6 hex D connector screws from the rear or ValveLink16 remove all of the screws from the top cover Only replace the fuse with a stan dard not slow blow of the same size One extra fuse is included on the c
25. shing tray the ValveLink can automate washing of electrophoresis gels and blots and devel oping of x ray or photographic film or prints You have complete control over which washing steps of your Western Northern South ern Coomassie Blue and Silver staining runs are automated and which are handled conventionally Unattended washing can save a busy lab countless hours of tedious monitoring Liquid Chromatography A ValveLink can be easily programmed to automate solution se lection in multi step ion exchange gel filtration or affinity chro matography runs with excellent reproducibility The ValveLink is especially useful in preparative work when a protocol has been established and programmed to repetitively isolate your valuable molecule Luer lock Valves and Syringe Reservoirs AutoMate also offers valves with luer lock fit fin tings for direct connection sae ce ce Bae E i ee t 5 E 5 of syringes This method 1 fs Ma oa Se pE imi ba E E S s ae TS eliminates wasted solu eS as ae s a 3 8S r 3 3 ot tion in tubing between E en ee pe Be Be g 5 jE the reservoirs and valves n E Small 2 way stopcocks are b lt included when this option is ordered Pressure Regulated Perfusion System AutoMate Scientific now offers a Pressurized Perfusion Upgrade with either four or eight channels for e Faster Switching e Steady flow rate e Microliter fine delivery e Microinjecting e Smal
26. ted a communications protocol com patible with the one offered by Axon Instruments All commands begin with ATn where n is a single digit from 0 9 indicating the device number being addressed The address is set with the Serial Address switch on the rear of the ValveLink All commands must be terminated with a carriage return lt cr gt Possible commands are ATnO lt cr gt Open ALL valves ATnC lt cr gt Close ALL valves ATnV v lt cr gt Open valve v ie AT6V 3 lt cr gt opens valve 3 on device 6 ATnV v lt cr gt Close valve v ie AT5V 1 lt cr gt closes valve 1 on device 5 All valve and mode numbers are 1 based instead of 0 based That is on a ValveLink8 they range from 1 8 and ValveLink16 valves range from 1 16 ATnM x x y lt cr gt Activate turn on modes ATnM x x y lt cr gt Deactivate turn off modes 16 All modes are separated by commas The modes that re quire parameters 4 amp 5 have them specified following a forward slash but preceding the comma that separates the next entry For example AT3M 2 4 11 5 3 6 lt cr gt activates modes 2 4 5 and 6 on device 3 with valve 11 specified for mode 4 preselect and valve 3 specified for Master mode 5 Again all valves are referred to starting with 1 The modes for purposes of serial commands are 1 Straight through TTL mode The individual TTL Inputs turn valves on or off This is incompatible with modes 2 3 amp 4 mode 1
27. tray inlets 5 Connect tray outflow to fourth upper valve barb 6 Connect this valve s outflow to vacuum trap 7 Plug numbered valve cables Operation With a ValveLink8 a researcher can turn valves on and off using eight TTL pins UL 8 from an interfacing board on a per sonal computer and their own custom programs See the de scription of Mode 1 in the next chapter to accomplish this using only three or four TTL outputs The ValveLink will open and close valves mirroring the computer signals low off high on with sub 10 millisecond accuracy ValveLink16 s have one additional TTL input to multiply the eight common inputs by 2 into 16 full channels of valve control ValveLink8 Standard female DB 9 connector ValveLink16 Female DB 15 connector Back panel diagram view Pins 5 4 3 2 1 TTL inputs 0 4 Pins 8 7 6 5 4 TTL inputs 0 4 N Pin 12 ground 9 Pin 6 15 ground a Pins 9 8 7 TTL inputs 5 7 Pins 15 13 Pins 3 TTL bank TTL inputs 5 7 select Manual Control Each press of a front panel pushbutton toggles its valve on and off The Reset button immediately turns ALL valves off and resets the ValveLink s internal circuitry The ValveLink16 includes 16 valve status LED s but has only eight pushbuttons like the ValveLink8 An up down switch toggles the ValveLink16 s push buttons between control of two banks of eight valves 3 way Valves The ValveLink also sup
28. tubing and manifold ports you may need to supply extra small tubing for this option Valve with 1 16 o d barb Short piece of 1 16 id tubing over valve barb Small diameter 1 16 o d Ano inserted inside valve tube and inserted inside manifold ports Manifold without edo tubing 1 16 i d holes Cutting the tubing at an angle will make insertion easier Cutting the tubing square however will minimize dead volume when inserted as far as possible into the manifold being careful not block at the point of convergence Remember to keep all tubing as short as possible Perfusion Pressure Kit sold separately 09 04 08 8 16 Press regulator knob down to lock Pull up to unlock Brass input connector hose barb size 1 4 i d Max input pressure 100 psi Individual air lines can be adjusted or turned off 0 Li 5 micron bowl filter A Be very careful with these Press bottom button to empty RELENE plastic hose connections E g E i B E E pl i i 2 i i f y Syringes may also be placed in a water bath for temperature control 2 way stopcocks and lure lock to 1 16 hose fittings are included for each channel to connect syringes to hose Hoses should continue from syringes to valves Multi barrel Perfusion Pencil sold separately Replacement Tips 04 04 xxx 4 into 1 Pencil with tip Part No Description 04 08 xxx 8 into 1 Pencil with tip 04 16 xxx 16 into 1 Pencil with t
29. ve tubing between drippers and valves long enough to raise and lower reservoirs 1 Assemble ringstand 2 Attach valve unit 3 Attach reservoir bracket s 4 Insert reservoirs 5 Insert short pieces of 3 16 i d tubing over tops of drippers then insert over reservoir nipples 6 Attach dripper tubing to upper valve barb cut as desired but see note above first 7 Insert green clamps on short pieces of 1 16 tubing 8 Connect tubing between bottom valve barbs and manifold 9 Connect flow regulator tubing to manifold outflow and your perfusion chamber downsizing if necessary 10 Plug numbered valve cables into ValveLink ports Calibrate flow rates by running one line into a graduated cylinder for a fixed period of time Teflon Micro manifold Use Your valves should be equipped or retrofitted with 1 16 o d outflow hose barbs Micro manifolds are shipped with a short piece of 1 16 o d tubing in each hole inputs and outflow You can either connect the valves to manifold inports with a piece of 1 16 i d tubing over both the valve barbs and short pieces of manifold tubing Valve with 1 16 o d barb l 1 16 i d tubing over barb and over manifold short tubing l Manifold with short pieces of 1 16 o d tubing in each port Or for even less dead volume use a short piece of 1 16 i d tubing over the valve barb and piece of smaller 1 16 o d tubing inserted inside the valve
30. veLink8 s and con trol up to 16 valves with a single 4 bit TTL source Set the first ValveLink8 to Mode 1 the second to Mode 2 and connect TTL input bits 0 3 from both ValveLinks to your digital output Bits 0 2 decode which valve is opened on the ValveLink selected by the fourth bit 13 high ValveLink in Mode 2 low ValveLink in Mode 1 As in Mode 1 TTL inputs 0 3 are used for addressing and no longer directly control their respective valves Example TTL input 0000 1001 opens valve 9 valve 1 on the second ValveLink8 43 Pre selected Valve Mode When Mode 3 is activated the user is prompted to press a button 0 7 immediately after leaving mode selection all LEDs will flash waiting for a single push button selection There after the valve selected is toggled on and off by TTL input bit 7 This mode allows a researcher to permanently connect a single TTL output to ValveLink input seven and select which of eight valves to activate with that single TTL signal This mode prevents input 7 from controlling valve 7 as it normally would unless valve 7 is chosen as the pre selected Mode 3 valve 4 Master Valve When Mode 4 is selected the user is prompted to press a button 0 7 immediately after leaving mode selection as Mode 3 This valve then becomes the master valve Thereafter whenever all other valves have been closed through push buttons TTL inputs or the serial port the master valve will automatically open This
31. ver for battery specifications VORSICHT Explosionsgefahr bei unsachgemass em Austausch der Batterie Ersatz nur durch denselben oder einen vom Hersteller empfohlenen ahnlichen Typ Entaorgung gebraushter Batterien nach Angaben des Herstellers See inside of front cover for battery specifications 26 Warranty AutoMate Scientific Inc warrants its products against defects of workmanship and or material for ONE YEAR from the date of sale Any product that fails to perform as specified may be returned freight pre paid to the factory with a written explanation of the problem for examination and repair or replacement If it is defective AutoMate Sci entific will repair or replace at our option the product without charge and return it to you If the examination indicates that non compatible fluid destructive environment accidental damage modification or abusive practices have occurred all labor materials and freight costs shall be at the expense of the customer Due to the nature of clinical laboratory applications AutoMate Sci entific Inc will NOT accept the return of any products which have been used with HAZARDOUS MATERIALS or harmful environment This warranty is in lieu of all other warranties whether oral or written express or implied In no event shall AutoMate Scientific Inc or its licensor licensees be liable for contingent special direct indirect or consequential damages for the breach of any express or i
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