LC/MS Pre-Installation
Contents
1. NOTICE Varian Inc was acquired by Agilent Technologies in May 2010 This document is provided Varian 310 320 MS LC MS as a courtesy but is no longer kept current and thus will contain historical references to Varian For more Quad ru pole Mass Spectrometer information go to www agilent com chem A 2 Pre installation Instructions ody Agilent Technologies Checklist NOTE Do not unpack the shipping cartons Check off each checklist box after satisfying each requirement as described in the instructions All requirements must be met before requesting installation NOTE If the site is not ready for installation when the Varian Representative arrives Varian Inc reserves the right to invoice for the Representative s time REQUIREMENTS Installation site is in compliance with all relevant safety regulations User Representative will be available during the installation and Varian certification period Entrance to the installation site is at least 92 cm 36 in wide Bench space is sufficient Bench can support system weight Bench does not vibrate Exhaust system is suitable Temperature maintained between 16 and 30 C 61 86 F 5 oO EE o oO oO EA o Relative humidity maintained between 20 and 80 o 5 EE o a Installation site is free of excessive particulate matter Specified electrical supply and power outlets are installed Nitrogen gas at least 99 pure regulator and2. caution All phases of preparing the installation site must conform to local safety electrical and building codes These codes take precedence over any recommendations in these instructions The customer is responsible for compliance Safety Safety is the most important consideration for instrument use Determine if the installation site complies with all relevant safety regulations Check the checklist box Installation site is in compliance with all relevant safety regulations 395411000 Rev 5 2 of 14 Pre installation Instructions Before the Installation User Representative Schedule the installation when the User Representative will be available One of the important duties of the Varian Representative is to familiarize the User Representative with the basic functions of the LC MS Check off the checklist box User Representative will be available during the installation and Varian certification period Entrance Before arranging delivery of the LC MS determine that there is sufficient clearance to move the shipping cartons to the installation site The largest shipping carton is 72 cm 28 in wide by 92 cm 36 in long If the cartons are to be moved with the pallet at least 92 cm 36 in clearance is needed Allow additional room for maneuvering the shipping containers around corners and through doors Vv Check the checklist box Entrance to the installation site is at least 92 cm 36 in The MS f
3. 6 to 12 in behind the system At least 76 cm 30 in above the system At least 30 cm 12 in to side of the LC Decide how to arrange the modules Use Table 6 and Table 7 to determine the width of the bench and the load the bench must support Regardless of module the bench must be at least 84 cm 33 in deep for the MS Table 6 Bench Width Calculation Worksheet for Your System Bench Auto Other 320 MS or Monitor Width sampler components 310 ms CPU ang Tota p p Keyboard m in Bench LC Auto Other 320 MS or Monitor CPU Total Load sampler Components 310 MS and Keyboard kg kg Ib Ib Vv Check the checklist box Bench space is sufficient TA Check the checklist box Bench can support system weight Vibration Ensure that the benches are free from vibrations especially those caused by equipment in adjoining locations Because the foreline pump s vibrates during operation it belongs on the floor below the LC MS not a on the bench with the LC MS Al Check the checklist box Bench does not vibrate 395411000 Rev 5 6 of 14 Pre installation Instructions Foreline Pump s The following table has information about the foreline pump s Table 8 Foreline Pump Information O ie o Weight or Load for optional 72 kg or 160 Ib foreline pump bench Diameter of hole in wall if 2 each 5 8 cm 2 3 in needed Width 35 6 cm 14 i
4. Rev 5 7 of 14 Pre installation Instructions Humidity The relative humidity of the operating environment must be between 20 and 80 with no condensation Operating the LC MS at a very low humidity may result in the accumulation and discharge of static electricity shortening the life of electronic components Operating the system at high humidity may create condensation and result in short circuits Varian recommends using a combination temperature and humidity monitor in your installation site Tl Check the checklist box Relative humidity maintained between 20 and 80 Particulate Matter Your installation site must not have excessive dust smoke or other particulate matter Particulate matter may block airflow vents causing the electronics to overheat Vv Check the checklist box Installation site is free of excessive particulate matter Power Requirements 212 LC Power Input 85 264V ac 47 63 Hz 60 Watts 100VA 216 LC 115V ac 15 20 50 60 Hz 250VA 230V ac 15 20 50 60 Hz 250VA For UPS assume 250W For 115V ac one 5 0 AT fuse 5 x 20 mm IEC 127 For 230V ac one 2 5 AT fuse All fuses supplied with the 216 LC instrument are UL listed and CSA certified The 216 LC must be used with appliances and power sources that have the proper protective grounding 310 320 MS The 310 320 MS MS requires a separate circuit and the outlet must have adequate amperage capacity and a reliable ground The power sou
5. 4 Ib 395411000 Rev 5 4 of 14 Pre installation Instructions 212 LC and HTS PAL Figure 2 shows a possible layout for the LC MS with 212 LC pumps and CTC HTS PAL Autosampler Figure 2 Suggested Layout with 212 LC and HTS PAL Table 3 and Table 4 give the dimensions and weights of the modules in Figure 2 Table 3 Bench Space Bench 320 MS or 310 MS Monitor and width Solvents 212 LC WithHtrspaL CPU Keyboard Total cm 172 4 cm in 68 15 in Total 111 4 kg 251 4 Ib The system requires a clean flat bench The bench must have enough space for the modules the mouse solvent bottles and such see Table 5 The bench must be strong enough to support the weight of the modules The area under the bench must be large enough for the foreline pump s If the bench is against a wall drill a hole large enough for the vacuum tubing of your pump s through the rear of the bench See Table 8 for more information Refer to the Varian LC Systems Pre installation Instructions part number 391483100 for information about LC modules Plan to put the monitor and keyboard on the same bench as the LC MS If the plan is to use a separate bench position them within 3 m 10 ft of the rear of the LC MS The CPU can go on or under the bench 395411000 Rev 5 5 of 14 Pre installation Instructions Table 5 Additional Bench Space Requirements Space Allowance 15 to 30 cm
6. The customer is responsible for setting up the preventive maintenance procedures and schedules contact the nitrogen generator manufacturer Any MS problems caused by poor gas quality are not covered by the Varian Warranty It is the responsibility of the customer to conform to all regulations regarding the installation and operation of the gas system The nitrogen supply uses in connectors Check the checklist box Nitrogen gas at least 99 pure regulator and gas lines are installed Air A compressed air gas supply capable of providing up to 2 L min of gas regulated to 80 psi with a two stage regulator is required as a nebulizing gas for negative ESI The customer is responsible to conform to all regulations for the installation and operation of the gas system The air must be clean and dry with less than 0 1 ppm total hydrocarbons including methane and have a 40 C dew point The air supply uses in connectors Check the checklist box Air less than 0 1 ppm hydrocarbons regulator and gas lines are installed CID Gas triple quadrupole only Argon is required as collision gas for MS MS work with triple quadrupole instruments The argon supply uses 1 8 in connectors Use a two stage 0 100 psi pressure regulator with a stainless steel diaphragm The purity of the argon must be greater than 99 0 Vv Check the checklist box Argon greater than 99 0 pure regulator and gas lines are installed Argon required only for t
7. The power cables for the CPU monitor and printer are approximately 2 13 m 7 ft long Figure 3 shows the power plug and outlet types with strict compliance with all regulations including electrical codes power cord color coding and appropriate regulatory agency certification marks Replacing or substituting power cords or plugs must be done caution 395411000 Rev 5 9 of 14 Pre installation Instructions ae Se wr l oS i Se cee K A 7 j Figure 3 NEMA 5 15P and CEE 7 7 Outlets and Plugs Installation Site Power In the United States the power supply to the installation site must be 100 120V ac and 200 240 V ac In Europe the power supply to the site must be 200 240V ac The power supply must be stable free of fluctuations due to slow changes in the average voltage or to changes resulting from surges sags or transients The voltage must meet EN IEC 61000 4 5 and EN IEC 61000 4 11 standards for voltage stability A measured ground to neutral potential of greater than 3 volts ac or dc indicates grounding problems that may need correction Evaluate any power source suspected of having noise problems with a recording type power line monitor before operating the system NOTE If concerned about the quality of the power install an uninterrupted power supply or a power conditioner or both see Table 12 Table 12 Maximum Power Consumption 320 MS or 310 MS without pumps 1200VA 320 MS
8. gas lines are installed Air less than 0 1 ppm hydrocarbons regulator and gas lines are installed Argon greater than 99 0 pure regulator and gas lines are installed Argon required only for triple quadrupole instruments Materials and solvents of specified grade are on site 310 MS ONLY Syringe pump similar to the Harvard syringe pump Varian part number 2732070100 Shipping cartons inspected for damage If there was any damage the damaged shipping carton procedure was completed Requesting Installation After preparing the site contact the Customer Service office in your region to schedule installation 395411000 Rev 5 1 of 14 Pre installation Instructions Contents ItrOCGUCTION EE TE Safety hena eee ov atic aaeeea ieia tees dats Before the Installation cccccccssssceeeeeeessssssteeeeeeeess User Representative cceeeeseeeeeeeeeeeeeeneeeeeeeeees Entrantes oer aie aaee arani E eatae enti aires Bench Space and Load escceeeeeeeeeeeteeees Vibration enirn iii a a a ives tenes Exhaust SyStem cccccccceeeeeeeeeeeeeeeeeseeeeeeeneeees Temperatures e cists aonb eet ees BSE eke Humidity boc eke ccsiecteccieesii cate ceedeniecied cut cbeveaseceed cones es Particulate Matter ccccccccccccccceeeeeceeeeeeeeeeeeeeees Introduction Power Requirement cccceeeeeeeeeeeeeeeeeeeeeeeeeee 8 Installation Site POWe cccceeseseeeeeeseeeete
9. ast three days Spare Parts The 310 320 MS LC MS and GC MS Quadrupole Hardware Manual part number 395417900 has a list of spare parts for routine operation Preventive Maintenance The customer is responsible for performing routine and preventive maintenance of the LC autosampler MS and data system If using a nitrogen generator perform the preventive maintenance to ensure that the nitrogen supply is clean and dry Any instrument problems resulting from a contaminated gas supply are billable and not included in the Varian Warranty Perform regular preventive maintenance to increase the life of the system to maximize system uptime and to optimize system performance Refer to the 310 320 MS LC MS and GC MS Quadrupole Hardware Manual for details The Varian Representative will describe and demonstrate these procedures during the installation Trademark Acknowledgment Microsoft and Windows are registered trademarks of Microsoft Corporation PEEK is a registered trademark of the Victrex PLC Company Lancashire England Other brand and product names are trademarks or registered trademarks of their respective holders 395411000 Rev 5 14 of 14 Pre installation Instructions
10. es 10 Qualified Computer Equipment 0ee 11 Gas Requirement cccssscssseeeeeeesesstteeeeeeeess 11 Materials and Solvents cccceeeeeeeeeeeeeeees 12 When the LC MS Arrives cceceeceeeeeeeeeteeteeees 13 Inspecting the Shipping Cartons 6 13 Unpacking and Installing eeeeeeeeeeeeeeeee 14 Spare Parts cos 2c a e ete eee ee eee ee 14 Preventive Maintenance cecccceeeeeeeeeeeeteeeeeeeeees 14 Trademark Acknowledgment cccceeeeeeeeeeeeees 14 The Pre installation Instructions are a guide for each requirement of the checklist Follow these instructions to ensure that the installation requires no more than the usual three days Have the completed checklist available to schedule the installation After the Varian Representative completes the installation and it is accepted samples can be analyzed After meeting all of these requirements contact the Customer Service office in your region to schedule the installation The LC MS operates reliably under carefully controlled environmental conditions You must provide suitable power sources operating environment materials and solvents Failures may occur if you use or maintain the system outside of the power and operating environment ranges and limits described in these instructions The Varian Warranty and Service contract specifically excludes the repair of failures due to such causes
11. n Depth 52 1 cm 20 5 in Height 48 3 cm 19 in Power cord plug CEE 7 7 or NEMA 6 15P Exhaust System Do not put the foreline pump s in an enclosed space Foreline pumps exhaust most compounds introduced into the MS and small amounts of oil vapor Provide an adequate fume exhaust system for the outlet of each foreline vacuum pump Each foreline pump must be vented at least 2 L min The API spray chamber must be vented at least 12 L min to a separate vent line Ensure that the exhaust system does not pull a vacuum on the API chamber Consult local regulations for the proper method of exhausting the fumes Al Check the checklist box Exhaust system is suitable Temperature The optimal operating temperature is between 16 and 30 C 61 86 F NOTE As installation site temperature increases system reliability decreases due to heat generated by electronic components during operation This heat must dissipate to the surrounding air for reliable operation The airflow around the system must be adequate The air conditioning system must maintain a constant temperature within the operational limits around the system Do not place the system near air ducts windows or heating and cooling systems The average steady state heat load for the MS alone is 6 000 Btu with a possible short term heat dissipation of 15 000 Btu during startup Tl Check the checklist box Temperature maintained between 16 and 30 C 61 86 F 395411000
12. or 310 MS with foreline pump s 3200VA Vv Check the checklist box Specified electrical supply and power outlets are installed 395411000 Rev 5 10 of 14 Pre installation Instructions Qualified Computer Equipment If the Varian MS Workstation software is to be installed on a computer not purchased from Varian the customer is responsible to ensure that the computer is adequately equipped and compatible with the operation of the data system and its communication interfaces Please check the current list of requirements available at the following web site http www varianinc com cgi bin nav products chrom gcms msws computer req Varian does not guarantee the function of the Varian MS Workstation software on computer hardware or operating systems that do not meet the specified requirements NOTE Contact your Sales Representative for a list of qualified equipment or for more information Gas Requirements Nitrogen A nitrogen gas supply that can provide up to a maximum of 12 L min of gas regulated at 80 psi is sufficient for ESI or APCI operation for one LC MS If there is more than one LC MS use a nitrogen supply that can deliver a maximum of 18 L min The nitrogen must be at least 99 pure with less than 0 1 ppm hydrocarbons and less than 1 oxygen It must be clean and dry with a 40 C dew point Use a nitrogen generator or a liquid nitrogen boil off to supply the nitrogen gas All nitrogen generators require regular maintenance
13. oreline pump s and LC are heavy To prevent Ne AUTION personal injury use appropriate moving and lifting techniques Bench Space and Load Use the following information to plan the layout of your LC MS The Varian Representative will unpack the cartons and put the modules on the bench Although the figures show a 320 MS the information also applies to a 310 MS Figure 1 and Figure 2 show suggested layouts for the LC MS with the 212 LC and different autosamplers The tables after the figures provide information about bench space and load The bench must be strong enough to support the weight of all modules wide enough for all modules and at least 84 cm 33 in deep for the MS 395411000 Rev 5 3 of 14 Pre installation Instructions 212 LC and ProStar 430 Figure 1 shows a possible layout for the LC MS with the 212 LC pumps and the ProStar 430 AutoSampler The autosampler belongs on the side of the 212 LC pumps not on top NOTE Do not stack an autosampler on top of the 212 LC pumps Figure 1 Suggested Layout with 212 LC and ProStar 430 Table 1 and Table 2 list the dimensions and weights of the modules in Figure 1 Table 1 Bench Space Monitor Benen Solvents 212 LC ProStar 430 27MS cpu and Total Keyboard 12 1 21 7 4 10 5 1 17 25 79 15 in Table 2 Bench Load Bench 320 MS or Monitor CPU Load Solvents 212 LC ProStar 430 310 MS and Keyboard Total kg 122 4 kg Ib 269
14. rce must be clean and capable of providing up to 220 240V ac 50 60 Hz 3 Hz 1 0 kW 395411000 Rev 5 8 of 14 Pre installation Instructions 310 320 MS LC MS The 310 320 MS LC MS requires the following Table 9 301 320 MS LC MS Circuits Power Plug and Outlet Types Table 9 lists the circuits power plug and outlet types of the 310 320 MS LC MS Component Circuit Power Plug and Outlet type MS 1 each 220V US 1 each NEMA 6 15P EU 1 each CEE 7 7 plugs Foreline pump 2 each 220V US 1 each NEMA 6 15P EU 1 each CEE 7 7 plugs Computer 1 each 110V US 1 each NEMA 5 15P 1 each 220V US 1 each NEMA 6 16P EU 1 each CEE 7 7 plugs LC modules 110V or 220V see appropriate user manual Table 10 has the power requirements for modules of the 310 320 MS LC MS Table 10 LC MS Power Requirements Module Max Current Draw Amps 200 240V ac 320 MS or 310 MS 5 Foreline pumps 5 per pump Use a separate dedicated power source for HPLC modules and additional instruments and equipment Never plug the mass spectrometer and the chromatograph into the same power source or the power source may overload Never use the free outlet on any of the power sources for equipment that draws more than 2A Table 11 has information about the power cords and plugs Table 11 MS and Foreline Pump s Power Cords 1 each 2 5 m 8 ft 2 each 2 0 m 6 ft 7 in Power Plug 1 each CEE 7 7 plugs 2 each CEE 7 7 plugs
15. riple quadrupole instruments 395411000 Rev 5 11 of 14 Pre installation Instructions Materials and Solvents The Varian Representative will prepare solutions to tune and evaluate the LC MS Please supply the materials Table 13 and the solvents on Table 14 Table 13 Materials Materia OOOO O each 2 Varian part number 2732070100 Table 14 Solvents for ESI or APCI Solvents for ESI or APCI Quantity each 2 4 liter bottles HPLC grade or better water new and unopened 2 4 liter bottles HPLC grade or better acetonitrile new and unopened Additional tubing is required to complete installation Most of this tubing is included in the accessory kits of the LC modules Additional tubing PEEK or stainless steel may be required for installation of special valves or modules For most analytical HPLC systems use 1 16 in tubing with an ID of 0 005 in Use 0 005 in ID or smaller tubing downstream of the sample injector and autosampler to prevent peak broadening Keep tubing as short as possible to prevent peak broadening and to minimize run time Vv Check the checklist box Materials and solvents of specified grade are on site 395411000 Rev 5 12 of 14 Pre installation Instructions When the LC MS Arrives Inspecting the Shipping Cartons After the LC MS arrives carefully inspect the exterior of the shipping cartons for evidence of any damage that might have occurred during shipment Inspect the cartons for the follo
16. s 5 Contact the appropriate Varian office to report the damage Systems are shipped either FOB Varian or FOB Destination The manner of shipment determines who is responsible for filing a claim against the carrier if the system was damaged in transit Most systems are shipped FOB Varian so any damages incurred in shipment are the responsibility of the purchaser and the carrier Contact the Varian office for assistance with filing claims and billing repairs If the system ships FOB Destination contact the Varian office and that office files a claim against the carrier Check the checklist box Shipping cartons inspected for damage If there was any damage the damaged shipping carton procedure was completed 395411000 Rev 5 13 of 14 Pre installation Instructions Unpacking and Installing The Varian Representative will review the Pre installation Checklist with the customer to ensure that the site requirements were met The Varian Representative will unpack and install the instrument and demonstrate the fundamental operation and maintenance procedures The User Representative must be present during the installation The Varian Representative will demonstrate that the system meets the performance specifications which are on the data sheet and any additional criteria explicitly written into the sales contract Plan to analyze samples only after the installation is complete and the conditions of delivery accepted The process requires at le
17. wing e Water stains e Cuts punctures or deep indentations MONITORED SHIPMENT IF TIP N TELL ARROW POINT IS BLUE THIS e Crushed corners or excessively abraded edges PACKAGE HAS BEEN ON ITS SIDE OR TIPPED n x 2 OVER IN TRANSIT e Blue beads in the Tip N Tell arrow point scsi ke vas mas Gu Canis bas Giese One Tip N Tell indicator label is on the exterior a OA E NOTON S oO of each shipping carton Read and follow the instructions on the label If the Tip N Tell arrow Ny TIp point is blue the carton was on its side or tipped F in transit and instrument damage may have EA TELL occurred PATENT NO 3 926 144 PRINTED IN U S A If no external damage is apparent sign the receiving documents Received but not inspected to indicate that the cartons were not opened If the customer receive obviously damaged materials without noting the damage on the receiving documents Varian will not accept the liability Do not open any shipping cartons The Varian Representative opens them during installation Move the shipping cartons to a warm dry and secure area near the installation site If a shipping carton shows evidence of damage do the following procedure 1 Report the conditions to the carrier when you receive the shipment 2 Note the damage on all copies of the shipping documents 3 Write a brief description of the damage 4 Ask driver to sign next to your comments to signify agreement with the observation
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