BP/08/2004 OK*** 10/24/05 12:02 PM Page 1
Contents
1. above steps contact your Labnet representa tive Before returning the pipette please ensure that the pipette is completely free of any chemical 13 radioactive or microbiological contamination which could pose a threat during transport and repair As far as it is possible clean the pipette 10 CLEANING AND STERILIZATION Cleaning External surfaces of the pipetting pushbutton the ejector pushbutton the handgrip the shaft nut and the adjustment knob may be cleaned using a cloth dampened in isopropyl alcohol The remaining parts removed from the pipette during pipette disassembly may be washed with distilled water or isopropyl alcohol Sterilization The pipette can be sterilized in the autoclave at 121 C for 20 minutes After sterilization the pipette should be dried and cooled to room temperature It is recommended to sterilize the pipettes in an autoclave with an initial vacuum and drying cycle prior to sterilization unscrew the shaft nut slightly in the BP2 BP1000 pipettes and unscrew the shaft slightly in the BP5000 and BP10000 After autoclaving these parts should be screwed tight again The precision of the results should not alter if the pipetting process and autoclaving are carried out as described in this manual Because a slight change in the accuracy of the dosage may occur it is recommended to check the calibration of the pipette after the ini tial first third and fifth autoclavin2. AND STERILIZATION 11 PIPETTE KIT AND ACCESSORIES 12 SPARE PARTS 1 INTRODUCTION The BioPette is a volumetric instrument desig ned to measure and transfer liquids precisely and safely It can measure and transfer volumes from 0 1 ul to 10000 ul depending on the model The BioPette has a digital counter displaying the set volume The set volume is visible in the window on the handle The setting of the volume is done by turning of the pipetting pushbutton knob Fig 1A2 or by turning of the black knurled adjustment knob Fig 1B in the right direction The volume range is shown on the pipetting pushbutton Fig 1A1 The volume range from 0 1 II to 10000 ul is covered by eight pipette models 1 Model Volume range pl BP2 0 1 2 BP10 0 5 10 BP20 2 20 BP100 10 100 BP200 20 200 BP1000 100 1000 BP5000 1000 5000 BP10000 1000 10000 Measurement and transfer of micro volumes BP2 BP10 DNA sequencing and enzyme assay applications BP20 BP100 Measurement and transfer of general aqueous BP200 and BP1000 solution acids and bases BP5000 BP10000 Measurement and transfer of large volumes The BioPette uses disposable polypropylene tips Fig 1E The disposable tips ensure maxi mum safety for the user and no cross contamina tion between samples To protect the user from contamination by the tips the BioPette is eq
3. 00 975 1025 250 00 Perform 5 aspirations weigh each one and calculate the average value of the aspirations e Calculate average aspirated volume in ul mul tiplying the average aspiration amount mg by the distilled water density coefficient ul mg which depends on temperature and pressure according to the following table Pressure kPa 95 101 3 105 20 1 0029 1 0029 21 1 0031 1 0031 22 1 0033 1 0033 23 1 0035 1 0036 24 1 0038 1 0038 25 1 0040 1 0040 If the average aspirated volume exceeds the permissible value the following should be done Temperature C 10 BP 08 2004 OK 10 24 05 12 02 PM t FLcftc Remove the pipetting pushbutton Fig 4A Warning The pipetting pushbutton consists of 2 parts the knob Fig 1A2 and the pushbut ton Fig 1A1 After removal of the pushbutton both parts are separated Holding the volume setting knob to protect it against rotation insert the calibration key into the cuts of the calibration screw Fig 4B Turn the key clockwise to reduce the aspirat ed volume or counter clockwise to increase the volume Fig 4C One full turn of the cali bration key changes the pipette aspiration vol ume by the amount given in the table Take out the key and fix the pipetting push button Fig 4D The pipetting pushbutton should be fixed by placing the knob
4. BP 08 2004 OK 10 24 05 12 02 PM Page 1 Labnet International I C PO Box841 Woodbridge NJ 07095 USA Phone 732 417 0700 Fax 732 417 1750 email labnet labnetlink com www labnetlink com BP 08 2004 OK 10 24 05 12 02 PM t FLcftc lt A 3 N F G J D L BP 08 2004 OK 10 24 05 12 02 PM Ppiecrette 4A 4B 4C 4D J A IS L lt 5A 5B 5C 2 1000 ul 5000 10000 ul 5000 10000 ul L STANDARD gt H0 C gt H1 m 2mm E 5 8 E E 5 5 C 8 BP 08 2004 OK 10 24 05 12 02 PM t Fcf c CONTENTS 1 INTRODUCTION 2 SETTING THE VOLUME 3 OPERATION 4 PRE RINSING 5 DENSE AND VISCOUS LIQUIDS 6 LABNET PIPETTE TIPS 7 RECOMMENDATIONS 8 RECALIBRATION 9 TROUBLESHOOTING 10 CLEANING
5. ade the performance of the BioPette Some Labnet tips are color coded and corre spond to the color on the pushbutton White tips 10 These tips are used for volumes between 0 1 ul and 10 ul They are used with the BP2 and the BP10 models Yellow tips 200 These tips are used for volumes between 2 ul and 200 ul They are used with the BP20 BP100 and the BP200 models Blue tips 1000 These tips are used for volumes between 100 ul and 1000 ul They are used with the BP1000 model White tips 5000 These tips are used for volumes between 1000 II and 5000 ul They are used with the BP5000 model White tips 10000 These tips are used for volumes between 1000 ul and 10000 ul They are used with the BP10000 model 7 RECOMMENDATIONS The recommendations below will ensure maxi mum accuracy and precision from your BioPette Make sure to operate the BioPette slowly and smoothly e The depth of immersion in the sample liquid should be the minimum necessary and should remain constant during aspiration The BioPette should be held in a vertical position e Change the tip when volume setting is changed or when a different liquid is to be aspirated Change the tip if a droplet remains on the end of the tip from the previous pipetting operation Each new tip should be pre rinsed with the liquid to be pipetted Liquid should never enter the BioPette shaft To prevent this Press and release the pu
6. ation should be repeated when the volume to be aspirated is changed or when a new tip is used BP 08 2004 OK 10 24 05 12 02 PM Pi Fcftc 5 DENSE AND VISCOUS LIQUIDS For moderately dense or viscous liquids com pensation is possible by setting the volumeter higher than the required value For liquids which are less dense than water compensation is possible by setting the volu meter lower than the required value Example to transfer 10 ul of serum using BioPette model BP20 Set the volumeter to 10 ul Aspirate a volume of liquid and measure it gravimetrically If the cor responding volume is measured as 9 5 ul the error is 0 5 pl Increase the volumeter setting by 0 5 II to 10 5 ul and repeat the measurement If the second measurement is not accurate enough adjust the volumeter up or down to take account of the remaining error When dispensing dense or viscous liquids wait one or two seconds longer at the first stop before expelling the residual liquid 6 LABNET PIPETTE TIPS Labnet tips are made from high performance polypropylene and their quality guarantees the precision and accuracy associated with the BioPette Strict control is maintained throughout the man ufacturing process to ensure the highest quality The accuracy and precision figures for the BioPette are only guaranteed when Labnet tips are used The use of inferior quality tips will seriously degr
7. e colo red black and red For the BP2 BP10 BP20 BP100 and BP200 pipettes the numbers in black represent micro liters and the numbers in red represent tenths and hundredths of microliters AN EXAMPLE FOR EACH OF THESE PIPETTES IS GIVEN BELOW BP2 BP10 BP20 BP100 BP200 1 0 1 0 1 red 7 2 7 2 a red L 5 red red 5 5 1 25 ul 7 5 pl 12 50 pl 75 pl 125 ul For the BP1000 BP5000 and BP10000 pipettes the numbers in red represent milliliters and the numbers in black represent microliters AN EXAMPLE FOR EACH OF THESE PIPETTES IS GIVEN BELOW BP1000 BP5000 BP10000 red C red o red 7 2 red 5 5 5 0 75 m 1 25 ml 7 5 ml The volume of the pipette is set by the knob in the pipetting pushbutton Fig 1A2 or by the black adjustment knob Fig 1B To attain the maximum accuracy set volume must be approached from a higher value If the desired value is lower than the previous value simply use the knob in the pushbutton Fig 1A2 or the black adjustment knob Fig 1B to adjust 4 BP 08 2004 OK 10 24 05 12 02 PM Bicbette the value down to 1 3 turn above the required set ting If the desired value is higher than the pre vious value use the knob in the pushbutton or the black adjustment ring to adjust the value to 1 3 turn above the required value The setting should then be performed in the following man
8. en Labnet tips are used SPECIFICATIONS Volume Accuracy Precision Mecel I 1 I 1 0 2 12 0 6 0 BP2 1 0 27 1 3 M X 2 0 1 5 0 7 Min 0 5 4 0 2 8 BP10 5 0 1 0 0 6 M X 10 0 0 5 0 4 MI 2 3 0 1 5 BP20 10 1 0 0 5 Max 20 0 8 0 3 Min 10 1 6 0 80 BP100 50 0 8 0 24 Max 100 0 8 0 20 Min 20 1 2 0 60 BP200 100 0 8 0 25 Max 200 0 6 0 20 Min 100 0 9 0 40 BP1000 500 0 7 0 20 Max 1000 0 6 0 15 Min 1000 0 6 0 25 BP5000 2500 0 6 0 20 Max 5000 0 5 0 15 Min 1000 X25 0 6 BP10000 5000 0 8 0 3 Max 10000 0 5 0 2 These specifications are obtained in forward mode using a gravimetric method with the tem perature of the distilled water tips and all other conditions stabilized between 19 C and 21 C Number of measurements minimum 10 The values given include all components of error due to both normal handwarming and the changing of the tip 3 Performance test The pipette is calibrated in accordance with EN ISO 8655 Performance can be verified by checking the pipette using the procedures outlined in the EN ISO 8655 standard The pipette design enables the user to perform the recalibration process according to the rules presented in section 8 2 SETTING THE VOLUME The volume display consists of three numbers and is read from top to bottom The three num bers indicate the volume selected and ar
9. expel any remaining liquid Fig 2D e Keeping the pushbutton pressed to the end remove the pipette by drawing the tip along the inside surface of the vessel Release the pushbutton Fig 2E e Eject the tip by pressing the tip ejector button Fig 2F It is only necessary to change the tip if a different liquid is being sampled or if the volu meter setting is changed Filters A replaceable filter installed in a seat in the bot tom part of the shaft is used in 5000 ul and 10000 pl pipettes Fig 3L The filter prevents the aspirated liquid from entering into the shaft and thus from polluting the inside of the shaft and the piston Using the filter is especially important when aspirating and dispensing large volumes of liquid If the filter becomes wet during liquid aspiration it should be replaced with a new one 4 PRE RINSING When pipetting liquids which have a viscosity and density different than water for example organic solvents a film of liquid is formed on the inside wall of the pipette tip This film can create an error Since the film remains relatively constant in successive pipetting operations with the same tip this error can be avoided by form ing the film before transferring the first sample This is done by aspirating a sample and dis pensing it back into the same vessel Since the film is already formed all of the following sam ples will have better accuracy and repeatability This pre rinsing oper
10. g cycles and then after every 10 autoclaving cycles BP 08 2004 OK 10 24 05 12 02 PM 8 _ Bbiotette 11 PIPETTE KIT AND ACCESSORIES Pipette kit The pipettes are delivered in the kits including Pipette Instruction manual Calibration key Ejector regulation spacers for pipette models BP2 BP1000 Ejector cap for pipette models BP5000 BP10000 Identification labels for pipette models BP5000 BP10000 e Lubricant Accessories Cat No Description P4803 10 10 ul tips racked P4803 200 200 ul tips racked P4803 1000 1000 ul tips racked P3998 5000 ul tips bulk pack L943 10000 ul tips bulk pack P3985 Carousel stand for 6 pipettes P3989 Linear stand for 6 pipettes 15 12 SPARE PARTS The spare parts indicated in Fig 3 4 and 5 A Pipetting pushbutton A1 Pushbutton A2 Knob B Adjustment knob C Shaft D Ejector F Shaft nut G Piston assembly H Spacer l O ring J Seal K Calibration key L Filter M Ejector cap N Ejector pushbutton can be ordered from a Labnet representative type of the pipette and name of the part for this pipette should be specified Warning The replacement of the plunger requires conducting of calibration proce dure according to section 8 Contact Labnet at 732 417 0700 fax 732 417 1750 email labnet labnetlink com BP 08 2004
11. g the proper sequence of steps Fig 3 No lubricant on the sealing elements Remove the tip ejector Unscrew the shaft nut remove the shaft piston assembly seal and O ring Rinse the removed parts in dis tilled water and dry thoroughly Lightly lubricate the inside surfaces of the seal and the O ring with the included lubricant Reassemble the pipette in the reverse order e Contamination of the inside of the pipette caused by extensive aspiration of chemically aggressive liquids or because liquid got inside the pipette Remove the tip ejector Unscrew the nut remove the shaft piston assembly seal and O ring Rinse the removed parts with dis tilled water and dry thor oughly Lightly lubricate the inside surfaces of the seal and the O ring with the lubricant Reassemble the pipette in the reverse order If you find an increase in the pipetting force which could happen after repetitive autoclav ing of the pipette Remove the tip ejector Unscrew the shaft nut and then remove the shaft piston assembly seal and O ring Rinse the removed parts in distilled water and dry Lubricate the internal surfaces of the seal and O ring with lubricant that has been included with each pipette Reassemble the pipette in opposite order Note All parts of the pipette can be auto claved see Section 10 The shaft of the 5000 and 10000 models should be autoclaved without the filter If the problem continues after carrying out the
12. he shaft nut is loose Fig 3F in the models BP2 BP1000 Tighten the shaft nut The sealing surface of the shaft is cracked or scored Remove the tip ejector Unscrew the shaft nut inspect the shaft and the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened In the models BP2 BP10 and BP20 the dam age of the shaft may also cause a damage of the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened To remove the tip ejector in models BP5000 and BP10000 remove the ejector pushbut ton Fig 3N and using a screwdriver unscrew the tip ejector by turning the screwdriver counter clockwise e Damage to the piston or seal due to prolon ged use with the aggressive liquids Disassemble the pipette as described above Replace the piston seal and O ring see Section 12 Rinse the inside of the shaft in distilled water and dry Lubricate the seal and O ring with the lubri cant that has been included with each pipette The replacement of the piston requires conducting of the calibration procedure BP 08 2004 OK 10 24 05 12 02 PM Page 18 t FLcftc Note The parts of BP2 and BP10 pipette should be lubricated evently with a mini mum amount of lubricant The pipette is reassemled improperly Disassemble the pipette and reassemble it observin
13. ner With the volume set to 1 3 above the required setting slowly turn the pipetting pushbutton knob or the black adjustment knob to the required volume If the knob is accidentally turned too far ie is set lower than the required value the process must be repeated The approach to the set vol ume must always be made in the order of decreasing value 3 OPERATION Place a tip on the shaft of the pipette See Section 6 for the appropriate tip Press the tip on firmly using a slight twisting motion to ensure a positive airtight seal Note Never handle a liquid with a BioPette which has not been fitted with a tip Aspiration Press the pushbutton to the first positive stop Fig 2A Holding the pipette vertically immerse the tip into the sample liquid The depth to which the tip is immersed in the sample liquid depends on the model BP2 and BP10 lt 1 mm BP20 and BP100 2 3mm BP200 and BP1000 2 4mm BP5000 BP10000 3 6mm Release the pushbutton slowly and smoothly to aspirate the sample Fig 2B Wait one second and then withdraw the tip from the liquid Wipe any droplets away from the outside of the tip using a medical wipe Avoid touching the orifice of the tip Dispensing Place the end of the tip against the inside wall of the vessel at an angle of 10 to 40 degrees e Press the pushbutton smoothly to the first stop Fig 2C Wait one second Press the pushbutton to the second stop to
14. on the arbor first Fig 1A2 and then the pushbutton Fig 1A1 Determine the average aspirated volume The average volume should be within the permissi ble range given in the table II the volume exceeds the values stated the recalibration pro cedure should be repeated When pipetting liquids with physical properties considerably different from those of water fol low the rules given in section 5 9 TROUBLESHOOTING If you notice an improper pipette operation identify the cause and eliminate the fault To do this follow the instruction in the sequence pro vided Replacement of parts should be required only occasionally and should not occur under normal pipette use Droplets of liquid remain in the pipette tip The tip is emptied too fast Decrease the speed of pressing the pipette pushbutton The tip wettability has increased due to exten sive use Replace the tip with a new one Droplets of air appear in the liquid aspirated into the tip The pipette tip immersion is too shallow 11 Immerse the tip deeper according to the instructions The pipette tip is incorrectly pressed onto the pipette shaft Press the pipette firmly The tip is damaged or worn out due to exten sive use Replace the tip with a new one The pipette incorrectly aspirates the liquid or liquid drops out from the tip The pipette tip is incorrectly pressed onto the pipette shaft Press the pipette tip firmly T
15. riate sensitivity has been used Volume checked ul Balance sensitivity mg 0 1 10 lt 0 001 10 100 lt 0 01 gt 100 lt 0 1 mg ul conversion factor has been taken into account ethe requirements given in sections 3 and 7 have been lulfilled If the above conditions are satisfied and the ac curacy error for selected volume given in section 1 exceeds the permissible value the pipette recalibration procedure should be carried out The recalibration can be performed within one full turn of the key to the right or to the left only 9 Recalibration conditions e Ambient temperature and the temperature of the pipette tips and liquid should be within the range 20 25 C and stabilized during weighing within 0 5 C e Measurements should be conducted using distilled water e Balance sensitivity should be suitable for the volume to be controlled Recalibration procedure Set the dose volume depending on the pipette volume according to the following table Range Preset Permissible Volume Chand AV for full turn of the Model of the pipette volume volumes volumes ul MI I calibration key ul 24 increments BP10 05 10 0 5 0 48 0 52 0 33 BP20 2 20 2 1 92 2 08 0 63 BP100 10 100 10 9 84 10 16 2 50 BP200 20 200 20 19 76 20 24 6 30 BP1000 100 1000 100 99 1 100 9 25 00 BP5000 1000 5000 1000 994 1006 125 00 BP10000 1000 10000 10
16. shbutton slowly and smoothly Never turn the pipette upside down Never lay the pipette on its side when there is liquid in the tip e Never force the volumeter beyond its recom mended limits e When pipetting liquids with temperatures dif ferent from the ambient temperature it is rec ommended to pre rinse the tip several times before use BP 08 2004 OK 10 24 05 12 02 PM 8 _ P FLcftc Do not pipette liquids with temperatures above 70 C When pipetting acids or corrosive solutions which emit vapors it is recommended to dis assemble the shaft and to rinse the piston and seal with distilled water after finishing the pipetting operation 8 RECALIBRATION The pipette is calibrated by gravimetric method using distilled water at the temperature 20 1 C according to EN ISO 8655 standard If during pipette operation you find that the accuracy error the difference between the real aspirated volume and the preset volume exceeds the permissible value given in the table in section 1 the pipette recalibration procedure should be carried out Before starting the recalibration it is necessary to check whether the following requirements have been fulfilled during error determination the ambient temperature and the temperature of the pipette tips and water was identical e the density of the liquid used was close to that of distilled water balance with approp
17. uipped with a built in tip ejector Fig 1D When using very narrow test tubes it may be necessary to remove the tip ejector It is simply removed by pulling down Adjusting Tip Ejector length models BP2 to BP1000 pipettes Fig 5A The interchangeable H spacers included in the box allow for adjusting the length of the tip ejector by 1 mm or 2 mm An HO spacer is inserted on a standard basis The outside shape of the spacer identifies the size change in BP5000 and BP10000 II pipettes Fig 5B The length of tip ejector is regulated by screwing in or screwing out the tip ejector stem with a screwdriver Turn the screwdriver counter clock wise to increase the length of tip ejector and clockwise to reduce the length of tip ejector The ejector can be adjusted in the range of 5 mm If above described method of ejector adjustment is not sufficient or the diameter of the ejector opening is too large to eject the tip it is necessary to put the ejector cap M onto the ejector Fig 5C BP 08 2004 OK 10 24 05 12 02 PM Ppiecrette Adjusting the length of the ejector will enable you to use your BioPette with virtually any tip found on the market The BioPette is a high quality instrument which offers excellent accuracy and precision The figures for accuracy and precision given in the following table were obtained using Labnet tips These figures are only guaranteed wh
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