LabSpec 5 user manual Page
Contents
1. HORIBA Scientific Lambda 4 or Converts the laser beam polarization from linearly polarized to circularly polarized None Leaves the laser beam with its intrinsic linear polarization 9 11 3 5 Fiber Entrance Select Fiber On or Fiber Off to select whether apeere the Raman signal is detected from the microscope 7 l icroscope Adjustment Camera or from a separate fiber optically coupled remote TinoOn Tinon Show Raman probe head such as a SuperHead Raman Polarization Ir FiberEntrance f Horizontal Vertical t Circular ff e Fiber On Fiber On In this mode only Raman signal which is delivered via a fibre optic to the FC connector on the underside of the XploRA platform will be detected You should select this mode if you wish to work with a remote Raman probe Later Polarization amp Fiber Off Lambda Lambda d None White Lamp Cancel Fiber Off In this mode Raman signal which originates from the microscope will be detected You should select this mode if you wish to make standard microscope measurements with the XploRA 9 11 3 6 White Lamp The White Lamp slider controls the intensity of an XploRA Commutations internal white LED to illuminate the sample when it oae is visualized using the internal Adjustment Camera C Tino0n Trino Off Raman Polarization i Horizontal Vertical Circular m Fiber Oni Lager Pola2. POENAE arrar EEEO EEEO EO 220 Red cursor PRCT ATE dicey ce a EEA EAS 165 Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual EAE T E E T A E T 220 Return to center of current WINdOW eeeeeees 92 Spectrum CUS ON lt xsadin canton Seanrnaevianiteandsbendnecsutoneaeaues 164 CSE MI Osorio cest sc tereess ECER 30 Adding information ccccccceeeeececeeeceeeeeeeeeeeeesees 31 Adding new information Categories cccccccseeeees 31 Deleting information categories ssccccesseeeeeees 32 T 1 e AEREE E IEA A A A 18 D Data AUTO SAVE serisinin aE See Auto save LOE cou eanevnnqasumnanabetcessaeenengecsieeccenp centers 11 Custom information ccccceeeeeeeees See Custom info Display Format and scale ss iicetasscisschiavieciavedseadaweniariasdedss 204 E a NE EE E AEE E E 216 Overlay mode Quick select single overlay mode 195 Select MO 6 sissacesssiccsiavsceaceassisadarcenesvacwssasxsaas 205 Spectrum format iscsi ccsacvasisctacedissaceredsianniieesevens 217 Window layout ceeeeeeeeee ees See Window menu E A E aE A E E SE ON O A 21 PING TOMAS orssed AERE 10 Get current range VAIUCS ccccesscccecsseeceeeeeeeeeeeeees 21 Wee Mel e E E ON EE NOE ETS 21 Manually modify spectrum using pencil tool 167 Mathematical procesSing cccccsssseeeeeeeeeees See Math Name tag in Control Panel ccccccc
3. cccccccsssseceeeceeeeeeeeeeeeeesaees 141 COMTI E orokre EEEE E 140 Delete variables ccceccccccsssseececcessceseeeeees 142 Searching Adjust peak position manually eessen 174 Label peak Manually ce eeccesseseceeeeeeeeeeeeeees 172 COONS srera er nerra EANN E E A 135 Remove peak manually c csseeccceessseeeeseeeees 176 SE ETET 136 PS UO Ola a E A E OEN 167 POC IOC erni 167 Photo bleaching timMe cccccsseececceceeceeseseeeeeseeeeeeees 35 Status bar indicator ceecccccesssseeeeeeeeeeeeeeeeeeeeees 199 TOE sonaa E 36 Page 256 HORIBA Explore the future HORIBA Scientific TUN ON sade rosin E E E 35 Pointer soc hecvcesvencasdsdawacxcabanewieewcedousned cowctewsevanas See Cursors Polarization Control laser polarization LabRAM ARAMIS s cccccssseeeceeeeseeeeeeseeeeeees 244 XPIORA 0ccccccssscceccessecccceeeceeceeeceseesecessaeeeeeees 240 Control Raman polarization LabRAM ARAMIS sscccccsssececceeessececeaseeessees 244 ADOR wets sau nee ea oanacgetuasnin ENEA 240 POIATIZOTS soris omiiia See Polarization Pressure units ss sssessesssesssesseresrrsersressrrsseesereserseeesee 23 Printing fuUnctionS essseeesssrereessrseesssressreeessseeessseeesssese 13 Print template Page ceccccccsssscccceeeeeeeeeeeeeeeeeaees 13 Profile Intensity profile across 2D image See Intensity profile Spectral See Multidimensional spectr
4. Autofocus Trigger Apply a script before after acquisition 000008 85 Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Trigger message WINCOW ccccsseecccceeeeeeeceeeeeeesaeeeeeees 32 U IES a eee 22 CUSTOM UNICS eroriren rE EOE 24 Pr ss re UNILS orisirisi naron nasan EE EAEN ARS 23 User log in profiles seicsersississerisocaretin srein 25 Changing a user profile password ccceeeeeeeeeeees 28 Creating a new user profile eosseenesseeeessrreesseee 27 Log in during a SESSION sssssssssssseesssressrrressrrrsssee 27 LORAIN GE StI re r EEE OESE 26 User NEVES cass iecncsareccsdwstincaudindeayecareseastavunadubecncestios 25 V Version NUMDES cccceeeccceseccceeesceceeecceeecessuecetsneceseness 88 Video images AspectratiO seerne isre r EA TA 207 Brightness and contrast ccccccccsseececeeeeseeeeeeeeees 212 Camera configuration esssessssssreesssrrresreressrrresseene 67 Default X axis CIS Pla ss saisecsisencessesasceawdsewensasninrnvdsons 209 Default Y axis GiSplay ccccsssssccecessececeeseeeesaeesees 210 Define multidimensional spectral array position 184 Extended video image 0666 See Extended video List of installed CAMELAS ccccccssseeeeeceseeceeeeeeeeeees 72 Overlay map score information eccerre 222 SAN PO arenen vamineceaiattanteanaieiatinteciavinalens 222 Selec
5. Mean spectrum From Selected aF a ccccccccsssecccceseceeeeeeeeeeeaees 219 From Selected POInts cccsscccccsssseeeeeeeeeeeeeees 219 Modelling cccccccssssseceeeeseeeeeseeeeeees See Modelling Overlay map score information on video image 222 Peak searching and fitting See Peak searching and fitting Restore Point window if it has been deleted 148 Save all spectra in array as individual files 11 Spectral profile Add spectrum to profile cccccseseeeeeeeeseeeeees 152 Create from individual Spectra cccccseeeeees 151 Delete spectrum from profile ccccseeeeeeeeees 153 Insert spectrum into profile ce ceceeeee eee ee ees 152 Threshold Gata ireren n 122 Multiply spectra c cc ceecccecessescceeeeesceeaeeeeeesaees See Math Multipoint acquisition Marking points ON video iIMAaBEeE cccccessseeeeeeees 184 Using XYZ Coordinate list ccccccccsssseeeeeeeeeeeeees 104 Creating a ISt csc Sonscsucscachincecsicpniracicamsinweiiendeeatene 109 Note about coordinates used sssesssseeeess 110 Saving d ta srren aeia 107 MultiwindOW ss ssssenssssereessseressreres See Extended range N Neutral density filter eeccccsssseeceeeseeeeeeeeeeeeeseees 227 NOVIMANIZ Os ciiaccinssansevosidasdisdaussdanbersntdusstastvestiasoiesetaiaarons 121 DV OO St aie AAE dense E A E E E anette 123 Automotive Test Systems Process amp Environmental
6. Note that in a non confocal mode you are analysing more molecules because the analysis volume is large and the signal level you observe will increase Some instruments have fully adjustable holes whilst others have fixed hole settings The controls on these are different 9 3 1 Control of fully adjustable hole Type in the desired diameter of the hole and press lt enter gt so that the value is registered Click on the left hand arrow to send the hole to a closed position 0 um diameter La Click on the right hand arrow to send the hole to its La maximum position Page 228 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific Click on the initialization icon to send the hole to its reference position and then back to the displayed diameter 9 3 2 Control of fixed position hole The drop down box lists all of the hole diameters available on the instrument 300m KODU bg Click on the drop down box to display the list and then select the hole diameter to be used Click on the initialization icon to send the hole to its gt reference position and then back to the displayed diameter 9 4 Slit The slit is used to define spectral resolution and should be used in conjunction with correct choice of laser wavelength and diffraction grating On most systems equipped with array det
7. acquisition time s i 10 Y _ Acquisition time s i 4 2 _ Number of accumulations 9 9 1 Real Time Display RTD acquisition time The Real Time Display RTD provides a continuous readout of the detector and is useful to adjust the fine focus of the sample to optimise Raman signal and to monitor whether a sample is degrading or burning The RTD is started using the 2e UB icon see section 4 5 1 page 95 for more details Page 234 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific Normally the RID acquisition time should be kept small in order to allow fast and continuous spectrum read out Typical values are in the range 0 2s 2s To adjust the RTD acquisition time simply type the Acquisition desired time in seconds into the box and press oq lt enter e gt e of 9 9 2 Acquisition time The acquisition time is the time taken to acquire a Raman spectrum Typically the longer the acquisition time the better quality the resulting spectrum The acquisition time set affects the Spectrum Acquisition lt i WE see section 4 5 3 page 96 and Mapping Acquisition 88 amp gt see section 4 5 4 page 96 Note that for an extended range spectrum acquisition See section 3 5 6 page 49 the acquisition time is the time taken for each spectral window within the full spectral
8. reduce the confocal hole diameter or decrease the acquisition time ie Stop the spectrum readout by clicking STOP 6 6 Zoom in on the peak using the Zoom icon in the Graphical Manipulation toolbar Use the cursor to locate the center of the 520 7 cm silicon Si peak If the peak is within 1 data point of 520 7 cm the calibration is acceptable If the peak is at a position greater than 1 data point from 520 7 cm the calibration must be adjusted To do this manually adjust by left clicking and Laser typing the laser wavelength displayed in the Laser drop down box in the Control Panel and press lt enter gt L 632 817 n Repeat this laser calibration procedure for all other lasers which are to be calibrated Page 250 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific INDEX 3 3D image display Adjust shape and perspective cccccsseceeeseseeeees 193 Format and display Options cccssssseceeeeeeeeeeees 224 Select 3D display format cccccsseeceeeeeseeeeeeeeees 206 A Absolute Positioning for multidimensional spectral arrays 98 Acquisition Multidimensional spectral array ACQUISITION time per POINL cceeeceeeeeeeeeeeees 235 Number of accumulations per point 235 Start ACQUISILION ecesna 96 Spectrum Acquisition time ss ss
9. 7 page 37 it is recommended to have a minimum of two accumulations so that the spike filter can detect and remove random spikes in the spectrum Page 235 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific Data from multiple accumulations will either be averaged or summed the desired mode can be selected from Acquisition gt Options see section 3 5 4 6 page 37 To adjust the number of accumulations simply type Acquisition the desired number into the box and press a lt enter e gt cy 10 ok gt 9 10 XYZ Coords Te Coord x 200 W a 1130 w z 10 W 2 The XYZ Coords section shows the current position in micrometers um of installed XY and Z stages It can be used for a number of functions Move the stage by a set distance by inputting a value rather than using the joystick Noting a reference position on a sample and returning to that position as required Measuring distances moved by the stage Switching between different stage configurations for example motorized XY stage and DuoScan O O O There are a number of variants of the XYZ Coords section depending on the system configuration The operation of each variant is described below 9 10 1 Using XYZ Coords The current stage position X Y and Z coordinates Z Coords are shown in the respective boxes The units are wd 200 CO v El micr
10. Automotive Test Systems Process amp Environmental Medical Semiconductar Scientific HORIBA HORIBA amore Scientific OD Yo 100 no attenuation DO 3 50 DO 6 25 D1 10 D2 1 D3 0 1 D4 0 01 Click on the drop down box to display the list and then select the laser wavelength to be used 9 3 Hole The confocal hole is used to define the spatial resolution and analysis volume of a measurement and should be used in conjunction with correct choice of microscope objective and laser wavelength to fully optimise a measurement Typically the confocal performance of a system improves i e the spatial resolution increases as the confocal hole diameter decreases Confocal analysis Confocal analysis means the measurement will be made with high spatial resolution suitable for analysis of true microscopic particles and thin layers with dimensions in the range 500nm 10um To analyze these types of samples the hole should be set to a small diameter Note that in a confocal mode you are analysing less molecules because the analysis volume is small and the signal level you observe will decrease Macro or bulk analysis For analysis of bulk powders and liquids or any sample where high spatial resolution is not necessary it is best to run the system in a non confocal mode By setting the hole diameter to a large value the spatial resolution will be low and the analysis volume of the measurement will be increased
11. Medical Semiconductor Scientific LabSpec 5 user manual O ODjECTIVE serrercornoir erini iorri See Microscope objective Open OIEA arsana E E A E 11 Options MENU essencassaincatavaensesstounadeseattccebsieusseminnenaniunrss 22 SALET ie EAEE E E IE ET E E E 23 Pressure UME scusassi a 23 Profil S sa wansenessacsdhstnaduceastnawuodandinesaaeaadintaneestaddewanvoss 25 TRENT FBS A A A E A E 29 E i EEE E EEE AAE A PEN A A T E 22 Overlay Data for display srsscccasttiantaviendciecrnies See Data Display Map score information on video image secese 222 P Poe AE a enn een nr ere rey en oe 18 Peak area Ey OUI O ees acta ccennata whacecds EE 177 By ENE CU OEE EE E E T 135 Peak pick cccseeeceseeeeees See Peak searching and fitting Peak searching and fitting cccceeecccesssseceeeeeeeeees 134 Add peak to SpectrUM ccccssseececeseseeceeeeseeeesaeeees 172 Display OPtiONS cccccssssecccceesseeeceeeceesaeeeseeseaeeees 143 Display peak ParaMetels ccccccccssssccceceeseeeeeeeeees 138 Fitting Adjust peak position and shape manually 174 Create image of fit parameters for multidimensional spectral array c0066 139 Fit 137 OPON isa cca caa eaten cewtenstpaideadesendcen beednenendtenenaateia 136 Remove peak manually ccccsssececcessseeeeeeeeees 176 Shape function Define CUSTOM SHAPEC ccseeececeeeseeeeeeeeees 144 eS EEP ENE PE EE A EAT 135 Variables Add variables
12. On i y i aman Folanzation FiberEntrance Microscope eye pieces and top camera are active eoan or veid r veneer Raman path is inactive a Laser Polarization Lambda Lambda d C None White Lamp Fiber Off Trino Off Raman path is active microscope eye pieces and top camera are inactive SS Cancel Irit Page 239 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA Explore the future HORIBA SoVentittse 9 11 3 2 Adjustment Camera Click on Show to activate the internal adjustment camera and to switch the microscope to Trino Off mode This camera is useful to visualize the laser spot on the sample White light illumination can be provided using the White Lamp slider bar to control an internal white LED or by using the standard XploRA microscope illumination options 9 11 3 3 Raman Polarization Select Horizontal Vertical or Circular to insert a polarizor element into the Raman beam path to control the polarization of the analyzed Raman signal Horizontal Only Raman signal which is horizontally polarized will be detected Vertical Only Raman signal which is vertically polarized will be detected Circular All Raman signal will be converted to circularly polarized light prior to detection Most spectrometers have different sensitivities for h
13. Video IMABES cccccccseeeeeeceeeeeeeeeeees See Video images Camera CCD camera for spectrum acquisition See Detector Video camera for optical visualisation See Video images Ela 6 aCe P EE A A E E E 11 Combine data Combine individual spectral windows See Extended range Confocal pinhole wv sicvecsveisctcasvevendinsetiandioeuseveardseastentess 228 Context help cee ceseeccccseesseceeceeeeeeceseeesseeeceesseeeeeeeees 91 CONG PINE errre nera RE 227 POGUES ION seais aainan iE 234 Data Name taB serris iniri n E EAE 234 E E E R EE AEE EREA 227 Le AEAEE T E II AA A AE E 228 Instrument setup sssessessesssssssresessresrsresresresressese 238 LEASE PPP E O A E A 227 Microscope ODJECTIVE ccccccseeeseceeeeeeeeeeeeeeeeaees 233 DPO NE eA E A EAE E EESE 231 S AEE EE EEEE E E AE AE EERON ET 229 Spectrometer ssssssseesresresreseosrrsresreresresresreeresres 230 XYZ COOKS sicsccssscrsassssassevsvsssessvossecsawssarsedsasansensssanncs 236 Copy and paste formatting cccccsssseceecsseeeeeeeeeeeeees 18 COA rE O E E A OE S 18 Cursors Display values positions cccccceeeeeesseeeeeeeeeeeeeees 201 POI rr E S NE AOAN 218 Integral CUTSOT ssiseirersirrricsirerisisreirersrnsisraa 177 Multidimensional spectral array analysis cursors Blue cursor PCV AUS reniei A 165 FO A Eee E 220 Configure ssccisevciiencecastacscednasssnadvesionssacnrbonssiasduesas 147 Green cursor PACTIN AUS ciecie a a 165
14. be aware that some of these may not be visible in your software 9 1 Laser The drop down box lists all of the laser Laser wavelengths available on the instrument Click on the drop down box to display the list and then select the laser wavelength to be used On fully automated systems such as LabRAM ARAMIS automated LabRAM HR or XploRA the necessary hardware and optics will switch automatically On manual systems such as LabRAM 300 LabRAM 1B manual LabRAM HR U1000 or T64000 remember to change the optics necessary to use the selected laser These optics include switching mirror s interference filter s and notch edge filter s 9 2 Filter Most systems have a number of neutral density filters available which can be used to reduce the laser power incident on the sample Typically these are necessary if the sample is sensitive to the laser power and burns degrades when the full power is used Note that the detected Raman signal is proportional to laser power so the lower the laser power the longer your measurement will need to be to obtain a good quality Raman spectrum These filters are motorized and will automatically be inserted into the laser path once selected The drop down box lists all of the filters available on the instrument These will be displayed either in optical density OD or percentage The following table shows the relationship between OD and values Page 227 Explore the future
15. detector is not saturated if the signal is too intense insert a filter in the laser path reduce the confocal hole diameter or decrease the acquisition time Stop the spectrum readout by clicking STOP Zoom in on the peak using the Zoom icon in the Graphical Manipulation toolbar Use the cursor to locate the center of the zero order peak If the peak is within 1 data point of 0 nm the calibration is acceptable If the peak is at a position greater than 1 data point from 0 nm the calibration must be adjusted To do this click on Setup gt Instrument Calibration to open the Calibration dialog window Adjust the ZERO parameter and then click the Apply You should see the peak move Continue to adjust the ZERO value until the band is within 1 data point of 0 nm Click OK to save the calibration values for the current grating and close the Calibration dialog window Resend the spectrograph to zero order and retake the spectrum This will make sure that your calibration changes have taken effect Check the zero order position again and if necessary make further adjustments change ZERO resend to zero check position etc until the position is acceptable NOTE The ZERO parameter should be changed in small increments e g 5 at a time Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual S Instrument Calibration Calibration Options Refe
16. point of 520 7 cm Click OK to save the calibration values for the current grating and close the Calibration dialog window Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Spectrometer Ei aM 520 7 cm i Wo ie 6 6 io Instrument Calibration Calibration Options Reference 20027 50 Counts 2nd Spectrum 60 45 3rd Spectrum f 4 4th Spectrum o Pixel Calibration Set Unit to Pixel Show Diode Show Central Pix Open Shutter Motor Calibration Motor Spectro Position all 654360 gt nef Grating 1800 Zero 8 000 Apply Koeff 0 002526 Cancel Help Page 249 HORIBA HORIBA asain Scientific 10 3 Calibration of Additional Lasers The process outlined above is used to calibrate the diffraction grating using the reference laser Additional lasers must be calibrated using the following procedure each laser must be calibrated individually using this procedure Select the laser to be calibrated from the Laser es drop down box in the Control Panel L 632 817 n Move the spectrometer to 520 7 cm to analyse Spectrometer the silicon Si first order Raman peak El un Jj L aF E E Acquire a spectrum of the silicon Si sample using the real time display RTD function Ensure the detector is not saturated if the signal is too intense insert a filter in the laser path
17. range For multidimensional spectral array measurements the acquisition time is the time taken for each spectral window of each spectrum acquisition in the array Minimum acquisition times are in the order of 0 2s 0 5s for standard Raman spectra and mapping experiments and lt 50ms for SWIFT ultra fast Raman mapping experiments There is no maximum value for the acquisition time since this will depend on the desired spectrum quality however the CCD detector will saturate at and above a particular signal level so it is important that the acquisition time is not so large that saturation occurs The saturation level depends on the specific detector installed on your system Modern detectors generally saturate at approximately 65 000 counts whereas older detectors may saturate at lower values Such as approximately 32 000 counts To adjust the acquisition time simply type the Acquisition desired time in seconds into the box and press d moo entere gt oqo _ gt i 4 E 9 9 3 Number of accumulations Acquiring multiple accumulations of data and averaging them results in improved spectrum quality However with array detectors such as CCDs and InGaAs arrays it is generally best to improve spectrum quality by first increasing the acquisition time When the saturation point is reached then it is worth increasing the number of accumulations Depending on the chosen mode of the spike or cosmic ray filter see section 3 5 4
18. used Click on the initialization icon to send the slit to its reference position and then back to the displayed width 9 5 Spectrometer The spectrometer control allows the wavelength nm or wavenumber Raman shift cm position to be selected With an array detector such as CCD or InGaAs array the specified wavelength wavenumber position lies at the center of the detector and a certain range either side of this position will be detected The precise range depends upon the spectrometer focal length laser wavelength and diffraction grating As an example if the spectrometer position is set at 1500cm then the lowest position could be 970cm and the highest position could be 2030cm The central position in the spectrum will be 1500cm The spectrometer position can be quickly controlled allowing you to monitor Raman peaks in a specific area of the full soectrum In most cases the detected range will be less than the full Raman spectrum range which is typically 100 4000cm If you wish to acquire a spectrum across the full Raman range then you should use the Extended Range mode see section 3 5 6 page 49 Type in the desired central position of the Spectrometer spectrometer and press lt enter gt so that the value is registered The current units will be M alj displayed next to the input box M07996 om Click on the left hand arrow to send the aj spectrometer to the calibration Zero Order posit
19. 239 Adjustment CAMELA cccccceseecceceeeseeceeeeeeesees 240 Fiber entrante cccisviscrciapacseaiavaesiassadsagansananteiadnacwa 241 Z WINET OSCO DG sj ccuxcecawedvn toawaundebesonouancevivevebwelvedwanenres 239 Polarizors TOC Aas coctessns ard atose E EE ai 121 FOI TASC A E E E A E 240 Zero order position For Raman ssiinscinscaracvcsicesapedventasssesseabansaansioone 240 Manual calibration seser See Calibration R iNitiAaliSC 0 cee ceceeecceecceeceeeceusseeseusceusseueeeuees 242 Send spectrometer to zero order 230 White LAID csshivcacwnsawadevsiacsientauelcwdiavenieseenetensiens 241 EGON AAEE AEAEE deur E E E E A E 168 Page 259 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA
20. D AM EE A O E E E 10 ACQUIS OD dei sesaucencatecaatnechaeracaamiencrasmeusensdteasstmanceaoua 30 I E EENE son EEEE AN 19 E a E E A caus E EE E ENA E 17 PUO a A EA E neaens 10 FS ID OE EEE A A A 88 PAA SSISTOINE jnsiiwedssndsdaiansisniadsievbicaetsavessnsiansiereiwosaees 76 ION Seear hota E EE OTE 22 O E E OE E E AN O E 82 SEU UID EAEE E PE E E A EE ocuncanes 73 NM ICIS a IE EE EEEIEE E AT EEA 66 WINDOW vnisiccsscsdnscusssabicscecsscntcvesatscowsandabedsnidensavacsdboes 86 Microscope ODjECTIVE cccccccsssseececeeeceeeeeeeeesaeasees 233 Modelling Display ETON Map eeri n sane eocecusnenss 156 Model SUM cecccccessseccceeeeececseeeeceseeeeeessaeeees 156 How to automatically create a model 006 161 How to manually model data cccceeeeeeceeeeeees 157 Using previously saved reference spectra 160 Load current SPECTFUM c cseeccccseseceeceeseeeeeeasees 156 Load multiple SOCCE A iscisccsnscscvessacssvedieasaaessatainessaeias 156 The Create factor analysis procedure 6 155 Set number Of FACtTOIS ccsseeccecessseeeeeeeeeeeees 155 SE Alt EEEE noses ait ounwedeseuse tune 157 The DCLS modelling procedure cccecsseecceeeeees 154 NGG IZOD sancon 154 Unnormalized cseecccccssssecceceeeeceeeeeceeeaeeeees 154 Threshold data cccccccssssccceccesseceeeeeeeeseeeeeessaeeees 155 Montage video IMageS ccccseeeees See Extended video Motorized sampl
21. G PV EVIEW sossarna r N 13 PRIDE SE UD e eeaece ees 13 P e E A EE O AOA E 13 Save laane E 11 SNE r E AE TEE 11 Save picture aS va ncsvcminsveintancaenevandeadantvendeaancnsrauderenbiness 12 ae L A E geen E E EAE OEE OET 11 Format 3D image display ccccccsssscceeccsseeeeceeeeeeeeeeeeeees 224 Axes and labels Fast SE LUNES errre rerni rer E EN 197 Properties ED ANG 2D display icsisrssisisniesiiisennan 213 JD CS OA nersini ana EAE 215 Copy and past nrrainn 18 UNS ON a NERA See Cursors Display Style sa vecviveinessseatvensncexianwea Acametonnvescinndoteons 204 Scale DEM AVIOUM vccrrsicacacsescucarcossevcsariantabardeateanderties 208 Fourier traNSfOrM ccccccesssececeeeeeeeeeesees See Smoothing G EE O PERE AE See Guided Operation Wizard Get current data range values cccccsseecceceeeeeeeceeeeees 21 Page 253 HORIBA Explore the future HORIBA Scientific Graphical manipulation toolbar ceecceeceeeeeeeeeees 164 Add baseline POiNts cccssseccccecssseceeeseeeeeseeeeeeees 180 Add CONS CAR i scaendcos instar dunevncvanseetvindevinsnosvatiantetnesannss 170 PNG POS eesin a N 172 Adjust peak ceecccccessecccceceeeececceeceeeeeeeeeesaeeeeeees 174 POS PEEN E E N AN 182 AKES D ea T 193 Circle NAIDU sarreretan a eai 189 Correct shapE sacii iia 167 Hor line MAP OIINS sccracciwessarvensdpaccasieadienerenscciasanarventas 187 WG aa EEEE E E EPE ETE E tease eee 177 intensity SIM senisircrnininiar
22. LabSpec 5 user manual HORIBA Scientific Show data limits When Show data limits is ticked the mapping area is Superimposed on the video image Show data points When Show data points is ticked the individual acquisition positions for the map are superimposed on the video image Show data name When Show data name is ticked the name of the image component is superimposed on the video image Show index When Show index is ticked the XY index of the individual acquisition positions for the map are superimposed on the video image Overlay images When Overlay images is ticked the Map or Score image is superimposed on the video image Note that the overlay mode in the Map Score window will be reflected in the superimposition Page 223 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Scientific The superimposed image will always be displayed in True Color whatever the palette setting in the Colors dialog window see section 8 6 page 210 Font Select the font style for the data index and data name text display from the Font drop down box Note that the font color is automatically assigned by LabSpec 5 to match the color of the object being superimposed Available for Video 8 18 Image3D The Images3D dialog window allows control of formatting and display options for 3D imag
23. al array User log in profiles See User log in profiles R Real time display RTD Acquisition WINING scrcrsnicecniiis 234 Detector image read out RTD mode CONE eer E ENE E 33 Start ACQUISITION sssirsicini r rR 96 Spectrum read out RTD mode Start ACOUISILION cesa TO 95 Reinitialize INStrUMENL ccccccsesseeeeceseeeeeeeeeeeeseeeeees 75 Relative Positioning for multidimensional spectral arrays 98 Report PUNCO sess cevcnsrreaxcesdicasnovecancdenacaseuerteaSenerecseevens 13 FRESCO ederran iin AEN EEEE ONE See Scale RESE dalarni RAN ENEE NEN 21 Resolution Controlling depth resolution with confocal hole 228 Controlling spectral resolution WIth diffraction grating cccccccssseeeeeseseeeeees 231 ATT SU ogee vices A OE A EAT 229 Rient chek MENUS cersesc 203 A OR AEE AE AAEE ITEE E AA AE TTT 213 Blue CU O arara 220 Centre CUNSONS sacoainccantesnceateeracsdeaeidssactuinaiawtantdianecins 212 IS ON E EE O EE danas 218 Fast SOLUINGS casi onietcoayanccanusannersakenseteasabanteasasea encevs 209 Format and SCC sclesicceactectapnneeseatesncaceanieacrsiansentonanees 204 Green CUrSOTF xociscnrtinsoenssanscencseihendeynssaneseaaropasstansvoree 220 nA ET E E E E EE NE N E E E O 221 Image COIOSLS cccccccsseccccesseceeeeeeececseeceeseeeeeeseees 210 E E S EEEE EET ROA 224 NOVAS GS Oea ER 222 K g ose NE EAE A EENE N E E A 216 Automotive Test Systems Process amp Environmental Medical Semicond
24. arinn 169 Line Mappe coses 188 Map analysis CULSOMS ssscccccseecceeeeeeseeeeeeeeeeeeees 165 Multiply by constant cccccccssseeeeeceeeeeeeeeeeeeeeeees 171 PO a N 164 Points mapping ssssssesssessessrrrssersressrsressrrssereseese 184 Polygon mapping ccnicwcsneviertivevidadinrermiienemenvanaess 190 Rectangular mapping ccccsssseccececseecceeeeeeeeeees 187 Remove baseline points cccsssccccssssseeceeeeeeeeeees 181 REMOVE Peak ccccsesecccceeecceceeeeseeeeeeeceeseeeeeeeaees 176 REMOVE SPIKC ccccseescccceeseccceeseesceeeeeeceeseeeeseeeeees 166 Seale SEIU cissi ninrin dar ATF EEEE 169 SPE UE a aa 165 SPIMGrEEN sciecesoenscavavexsenssconsaneotasnineadicnadesoaisencsoasans 165 SPIRE d essiri ana E NENO 165 Ver line MaPpPiINg cccccccsssssececcesseeeceeeeeeeeeeeeeees 190 LOOM aa A A EAE E E 168 Graphical user INtErfaCe ccecccccessseceeeeeeeeeeeeeeeeeseeees 8 Guided Operation Wizard ccccccccsseseceeeeeeeeeseeeeeeees 82 H Help Context help sssssnssssenessserersssrerssserssseresssrresseeressne 91 Online TOPICS ecicesisdaiapissactsnicetisvotereianadsenianteraaianeeneens 88 Help NG sann earn EEEE N N 88 About LADS POC iz ciscdiascansdacatenebasvancinansaveedandsanddenntatiee 88 Help COGS casur 88 ANN ITET A E T E A AE 88 j LEON Dai eroriren E TERR 89 BIE O reser sees aac nsencesunene et eee esee cay 89 Cursors and data information iCONS
25. csseeeeeeeeeees 234 Normale serisinin 121 CS p R EEA A E EA E E E E A 11 Peak searching and fitting See Peak searching and fitting Range and values ceseccccceseceeeceeeeceeeeeeeeesueeeeeees 20 VG OM aa E A 92 Redo the last ACTION ciscscssssscrnedaisenverseivanedesververesvannes 18 BLEE a E A E A E A AA us 21 Restore deleted data cccccsssccccsseeceesseeceeseeeeeeees 18 eS AMM acct T A E E T E E T EAT 11 SIVE AS g E E 11 Save DICE AS nccnirsnnn ta 12 SLE AE E N E EENE A EE ne See Scale Selecting data objects Using data list cancrnct ce cncutycestecocswatespeesteesteneveennnts 19 Using selector radio tags ssesccccsseeeeeeeeeeees 198 Split spectral array nsosssenossrsenssssenssreressreressseress 11 Undo the last action ccccccssseccceeeeseeeeeseeeseaeeeeeees 18 DOO POE IEEE LENEE AA A N E 121 Data Daere iai EE al ours 195 Fast graph settings ccccccccssseeccceeesseeeeeseeeeeeeeees 197 Page 252 HORIBA Explore the future HORIBA Scientific Quick select Single or multiple data ViCW ccccessseeeeees 195 Selector radio tags sccccccsssseceeeceessceeaeseeeesaeaeees 198 Data MENU esera concausstaned ueavan easier danteerdoeneushahisavesess 19 Data VAN suncricesaosrencemareemencseeaisenss vers neS 20 Database S arch ccccssseeccecesseeeeeeeeeeeees See Spectral ID DENOI E eera ERO See Smoothing Derivative PINGS E EE A EEA E T te
26. d for fast Raman mapping applications Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Aramis Measure Location Polarization C Macro 30 Laser Raman C Vertical fe iiae Horizontal Horizontal C Circular f None Scrambler Eon Vertical f External Visualization f Video On C Video Off f Point Mode TrnoOn Trino Of FTIR Top Video Detection UY Visible CCD 6 gt PMTVIGA fe UY ZIR f Visible Cancel Help f Line Mode Page 245 HORIBA HORIBA Scientific 9 11 4 5 Detection Select CCD or PMT IGA to control the optics for eee multiple detectors mounted on the LabRAM 7 T easure Location Polarization ARAMIS E Macro si Lazer Raman Boon C Yertical C Vertical CCD O vice f Horizontal Horizontal In this mode the standard CCD detector will be 7 External Circular None used for the analysis M Scrambler Wisualization gt r Measure PMT IGA mie Bone ean Point Mode In this mode the detector mounted on the second TrinoOn f Trina Off C FTIR Aa ei port will be used for the analysis Typically this Top Video detector will either be a photomultiplier tube PMT Detection UN visible ae re x a InGaAs array IGA or a second specialized CCD SES wei PU Vg tal Cancel Help 9 11 4 6 UV Visible Select UV IR or Visible to control the dual path meme opt
27. e pieces and top camera are active for transmitted white light illumination only and inactive for reflected white light illumination Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Measure Location Polarization C Macro 90 Laser Raman C Wertical O lige Horizontal Horizontal ie C Vertical f Circular f None Scrambler Visualization Measure Video On C Video Off f External f Point Mode f TrnoOn Trno Of C FTIR Top Video Detection Uv z Visible CCD PHTVIGA f UY IR C Visible Cancel Help f Line Mode Page 243 HORIBA Explore the future HORIBA SoVentittse 9 11 4 2 Polarization Select the required polarizor element to control the polarization of the laser beam and the analysed Raman signal Laser Vertical Allows the laser beam to be linearly polarized in a vertical orientation Laser Horizontal Allows the laser beam to be linearly polarized in a horizontal orientation Laser Circular Converts the laser beam polarization from linearly polarized to circularly polarized Raman Vertical Only Raman signal which is linearly polarized in a vertical orientation will be detected Raman Horizontal Only Raman signal which is linearly polarized in a horizontal orientation will be detected Raman Circular All Raman signal will be converted to circularly polarized light prior to detect
28. e stage Move by inputting coordinates cccceeeeeeeeeeees 236 Switching stage cccccccssssecceeceeeeceeeeeceeeueeeeeeeeees 237 Viewing coordinate POSITION ceececceeeeeeeeeeeeees 236 Motors Calibrate erriei eiaa EE 74 Reinitialize 0 eee ceeeecceeeeeseeceeceeeeeceeecceeeaeeeeessaaseees 75 Multi batch processing ccccccsssssesececcesesseeeeeeeseeeees 23 Multidimensional spectral array Acquisition Measurement OFC ceccccccssseceeeeeeeeeceeeeeeeees 99 Changing the Order cssscccssssseeeeeeeeeeeees 101 Paramete S ude ccctitnonsesaicoansyoiphodepasemineseaestsevesteenseers 97 Page 255 HORIBA Explore the future HORIBA Scientific Start ACQUISITION siii 96 Use SWIFT for ultra fast acquiSitiOon 0066 99 Adding a new array dimension sseccceeeeeeeeees 100 Baseline correction ccccsesscccceceseeeeeeeeeeeeeeeeeees 120 Correct spectrum in array sssssssessesssessrrssesserssee 148 Cursors for aray analysis sccceeseeeeeees See Cursors Define position on video image OTS asia ore E E E A E 189 Line DSM dll sesanan en neice 188 Horizontal smerdai 187 a e E E E A E A 190 POIT on Ee N 184 PONEO erennere EEE 190 Rectangle erena 187 Deleting an array GIMENSION sseecceeeeeeeeeeeees 101 Factor ANALYSIS cccssescecceeeseeeeeeeeeeeees See Modelling Image POPE ItieS cceccssscceseccessceeseeneeees See Image
29. eaching time Explore the future Automotive Test Systems Prooess amp Environmental LabSpec 5 user manual SCANNING GOVICE ccceeeccceseeccceeececeeeeeeecesseeceteneeenen 40 Sh tter MODE sariaren iia 43 Signal ModE ansen chad sansuesonanaienionen 41 Spectral range ccescccesseeeeeees See Extended range Spike Filter cccccsessssecceceseeeeeeeeeeeees See Spike filter TCIM GALES scsi canssscicdicestivveentisinisnanseciss See LabAssistant PES CCU a eaoin ee See Math Adjust intensity ceccccseeeeeeeeeeeees See Extended range Alignment Use internal alignment di0de cccccceseeeeeeeeereees 74 ARAMIS s0eesccccecceeesseeeccceeeeeeeeeees See LabRAM ARAMIS ASPE TTO oE 207 Auto exposure CONE UTE certes ernus en n EE 65 TOOTE e 42 TUN O eeren anae A OA EE E E OTA 42 AUTO SIVE aisina E A 47 WPUUIRIT GUE oeaan E 49 PEON OU sense es N EA E E A E E 49 Auto SCANNING sss sssessssessserresrerssere See Extended range Autofocus COMMPGUNE oriirreniternronrin ee riepa ee ai ei 60 Set a focus ONSOUs sisxiasscvssainecaceiasvievsisdeeeseiarodcesicouises 40 Set when Autofocus is applied ccccceceeeeeeeeeeees 39 Status bar indicator vaviccsvecsiconsesenssscnxndsaveavascareonncede 199 The Autofocus Procedure cccccessccceeeeeceeeeeeeeceeees 60 TUNE O FE oirir a E E N NET 40 TON ON vo euasnitast oqceusnvesstunesaresuenaeteortes 40 Axes ELI A E A E E es See Format B Background subt
30. eatmecneaes 126 PONE 126 Detector Accumulation Mode esssssesssensssseeessssrersrrresssrresseeee 37 Binning FaCtOF cceecccceeseeeeeeeees See Binning factor CONTET O arer E EAEE O EKTA 57 Dark subtract sasacenssasncesevisnsnraviceseaeussadcndatrcentatoncndes 41 Image real time display RTD CONEUTE euor srann rins n een o E Ni 33 Shutter ModE sereias innan ENN 43 Signal mode sssseossnnssssenesssseersssrersssrrssseressrrressreressne 41 Switching detector serrierenrecrsenriireis ren 201 Temperature Set temperature ssessessessessesessresresessesresresres 59 Status bar indicator cccccccsssseseeeeseeeeeseeeeees 200 Warm up to room temperature essssssssssssssseses 66 Diffraction Qrating ccccccccsssseccecesseceeeseceeseeeeeeeseeees 231 Manual xchange ccccccsssscccceessscceseeeceeseeeeeeeseees 232 Bo 9 a or teeta ante tener ane ere nee nee me er ane See Data DUO Ce A E E datedat teense ONE 110 Modes of operation POE a E tous cenaneusetuetednesencees 113 cer ALD A EEE T T E 111 Scanning ZONE erenn 110 DE a A E EE O E E E 111 E ESAL A E AA EEE E E E E E 17 CODY aae E EA E EO EEN 18 OE E E E E EE LE ENE E PE EE EES 18 EA EEEE IEE AE A A A AA TAA 18 P aS UE orrori pen sterner eaeeeeesas 18 PRO CO OEE E TE AEE ET A E T T ETS 18 PROS LOM A ANAE E A O EE E EA 18 U a EE EEA E E AE 18 Extended range snecssiermeenen ne das 49 Adjust baselines of individual windows After acquis
31. ectors such as a CCD or InGaAs array the diffraction grating see section 9 6 1 page 231 has a more significant effect on spectral resolution than the slit The slit should only be adjusted once a suitable diffraction grating has been selected Typically the spectral resolution of a system will increase as the slit width is decreased High spectral resolution obtained with a narrow slit width allows subtle changes in a spectrum to be confidently analyzed and close lying peaks to be separated Some instruments have fully adjustable slits whilst others have fixed slit settings The controls on these are different 9 4 1 Control of fully adjustable slit Type in the desired diameter of the slit and press lt enter gt so that the value is registered Click on the left hand arrow to send the slit to a al closed position 0 um width Click on the right hand arrow to send the slit to its P maximum position Page 229 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA es Scientific Click on the initialization icon to send the slit to its reference position and then back to the displayed width 9 4 2 Control of fixed position slit The drop down box lists all of the slit diameters Slit available on the instrument i Surm z 100ur 200um Ls Click on the drop down box to display the list and then select the slit diameter to be
32. er if the laser is turned on and active I rt Setup Click on the toggle switch to open or close the Aramis Ararrs ae gt lt lt seun Laser shutter Laser shutter closed open Page 238 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific 9 11 3 Control of Hardware Options for XploRA The XploRA Setup window allows control of the following optional items Trinocular head on the microscope Raman polarizors Laser polarizors Internal adjustment camera White lamp illuminator for the internal adjustment camera Fibre entrance O O O O O AplioRA Commutations Microscope gt r Adjustment Camera C Trina On f Tino Off Show Raman Polarizatian FiberE ntrance i Horizontal Vertical t Circular C Fiber On Laser Polarization Lambda Lambda d C None Rite Lanip f Fiber Off ee eee Cancel Irit Please note that depending on the configuration of your system certain items in the Setup window may be greyed out The XploRA Setup window is opened by clicking yploRA z on the Setup button gt 9 11 3 1 Microscope C Fiber On Select Trino On or Trino off to control the optical XploRA Commutations path to the trinocular head Microscope Adjustment Camera C Tinon Trino Off Show Trino
33. es It should be used in conjunction with the Colors dialog window see section 8 6 page 210 Image SD r a Tope lp 3 lt gt Help Gradation soe 2 Cancel Color Y Fill C IA Type Select the display mode from the following options o Line The line color is selected from the Line drop down box Intensity a u Page 224 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific o Line and Fill The line color is selected from the Line drop down box The line display can be removed by setting the line color to None When there is no Gradation selected i e X Y and Z gradation boxes are all unticked the fill color is selected from the Fil drop down box The example shown right is displayed without gradation Intensity a u When Gradation is selected i e one or more of the X Y and Z gradation boxes are ticked the graded fill color is set according to the palette selected in the Colors dialog window see section 8 6 page 210 The example shown right is displayed with Z gradation using a 3 greyscale palette Gradation Select the axis or axes direction for graded fill color o X graded fill from low X axis value to high X axis value 100 100 Page 225 Explore the future Automotive Test systems P
34. ics in the LabRAM ARAMIS which allow T oe i o Measure Location Polarization optimised detection in either the UV IR or visible Peme pene Seer spectral regions C Retro C Vertical Vertical fe ohana f Horizontal Horizontal UV IR External f Circular e None In this mode mirrored optics are used which are Scrambler optimised for UV and or infra red IR analysis ies a PE f Video On C Video Off l f Point Mode Visible TrinaOn Trina Off C FTIR C Line Mode In this mode achromatic lense optics are used Top ideo which are optimised for standard visible analysis Detection CCD f PMT IGA Page 246 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific 10 Appendix Manual Wavelength Calibration of LabRAM Systems The procedure outlined below can be used to manually calibrate the LabRAM systems including LabRAM 300 LabRAM 1B LabRAM HR LabRAM ARAMIS LabRAM INV LabRAM IR and LabRAM IR The full calibration procedure uses two software parameters o ZERO for calibration of the diffraction grating s zero order peak position 0 nm KOEFF for calibration of the Raman peak position Note that this calibration procedure is a diffraction grating calibration and should be repeated for all diffraction gratings on the system The software will retain individ
35. ings available on the instrument Mig 1800 gt h hiso l Click on the drop down box to display the list and a BOO N then select the diffraction grating to be used fe sampleA fe sample A A message will prompt you to now manually select the desired diffraction grating Typically this is done on the instrument using a push pull bar or other switching device Once the diffraction grating is selected click OK 9 6 1 2 Selecting a grating motorized turret The drop down box lists all of the diffraction gratings available on the instrument Wy 1800 gt h 150 Click on the drop down box to display the list and F jau LE i N then select the diffraction grating to be used The sample A fe sample_A diffraction grating will now be moved into position by the software 9 6 1 3 Selecting a grating for manual exchange The drop down box lists all of the diffraction gratings available on the instrument 1800 l ani F 1800 B 300 100 sample A F j My Click on the drop down box to display the list a Gratings available for manual exchange are listed Re with either A gt or B gt in front of their identifier This implies that they are intended to be exchanged in the A position of the turret or the B position of the turret The first grating listed in this case 1800 is in the A position The second grating listed in this case the 600 is in the B position This exam
36. ion Raman Scrambler The polarization of the Raman signal will be scrambled removed prior to detection 9 11 4 3 Measure Location Select the required measurement location on the system including the microscope macro chamber and external fibre optically coupled probes Macro 90 In this mode Raman signal which originates from the macro chamber in a 90 orientation will be detected You should select this mode if you wish to analyse bulk materials in the macro chamber using a 90 orientation Retro In this mode Raman signal which originates from the macro chamber in a 180 back scattering retro orientation will be detected You should Aulomotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Aramis Measure Location Polarization C Macro 30 Laser Raman C Vertical Go Horizontal Horizontal C Circular f None Scrambler Visualization Measure f Video On C Video Off Fete O Vertical f External f Point Mode TrinoOn Trino Of FTIR Top Video Detection Uv Visible CCD PMTVIGA fe UY AIR f Visible Cancel Help f Line Mode Aramis Measure Location Polarization C Macro 90 Laser Raman C Vertical O ime Horizontal Horizontal C Circular f None Scrambler Wisuallzation Measure f Video On C Video Off peers O Vertical f External f Point Mode TrinoOn Trino Of FTIR T
37. ion 0 nm Page 230 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific Click on the right hand arrow to send the spectrometer to its maximum position Note that the maximum position will depend upon the spectrometer laser and diffraction grating Click on the initialization icon to send the spectrometer to its calibration position and then back to the displayed position 9 6 Options The Options section allows you to specify the diffraction grating microscope objective and data name tag Mn 1800 lt Diffraction grating FT E 00 Microscope objective Re sample_ Data name tag 9 6 1 Diffraction grating The diffraction grating is the dispersing optical element within the spectrometer which splits the spectrum into its constituent colours Each grating offers a certain level of dispersion which affects the spectral resolution of the system and wavelength coverage which affects the spectrum intensity and compatibility with particular laser wavelengths Gratings are typically classified by a number of grooves per mm for example 300gr mm low resolution or 3600gr mm high resolution The higher the number the higher the achievable spectral resolution Each system is equipped with different diffraction gratings which are chosen to take into account the available
38. isualizati N cnicndssanaatGanianacatendeanccaadenoansenisedsbaneae 243 Instrument SetuP xc cctseacsccastenisevanensduassntueateencsacassenvs 238 Turn on laser esseesrsiersisirrerssssrrisserssssrsssseiriserisrsssses 238 LaNg AS E siasa r E N S 88 Laser Create Macro SPOt cccsccsscceseeseeeeeenes See DuoScan Polarizati N ssicisvatsdescnsendisdsieatusontnnaiices See Polarization Power UET orerar EAE Ti 227 Reduc POWED senscssnwansnaannn aana 227 Scan spot over ALEA cceccseceeceeeeeeceseees See DuoScan Selecting laser wavelength c csssccccssseeeeeeeeeees 227 Set laser spot marker position on video image 67 Turning on LabRAM ARAMIS s scccccccssseeceeeeeeeeeeeeeeeeees 238 ADOR A eE RE T 238 Layout of LabSpec 5 software esssseessseresrrssrerserererere 8 Ee ae EE EEE EE T EE 216 LineScan Activate LineScan for XY Mapping ccccseeeeeeeeees 40 Scale the video image for the scan size sesen 71 Log in PASSWOLC cccseeeeeeeeeees See User log in profiles Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual M IVIAD vecsiededennesvecctwecs See Multidimensional spectral array A E ease tet E tees AE A oss ce cet ETE E T 130 Drag spectrum with mouse to add constant 170 Drag spectrum with mouse to multiply by constant E ea eect sata cases ee 171 Mathematical Manipulation ccccseseeeceeeeees See Math ICI
39. laser wavelengths and requirements for spectral resolution Most instruments have grating turrets with multiple gratings attached This allows you to quickly switch from one grating to another for example to switch from low resolution to high resolution analysis The following list shows the standard maximum number of gratings available on the turret 1 grating fixed position HE spectrograph Axial spectrograph 1 grating U1000 164000 2 gratings manual switching LabRAM 300 1B and IR IR and INV configurations 2 gratings motorized switching _LabRAM HR and IR IR and INV configurations 4 gratings motorized switching _LabRAM ARAMIS and IR and IR configurations XploRA lt is also possible to manually exchange diffraction gratings on the LabRAM HR base unit U1000 and T64000 For example a LabRAM HR could be equipped with three diffraction gratings in total Two would be mounted on the motorized turret and the third would be kept in a box by the system One Page 231 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA os Scientific of the gratings on the turret could be exchanged with the third grating if desired Manually exchanging grating typically takes a few minutes to complete and there is no optical realignment required 9 6 1 1 Selecting a grating manual turret The drop down box lists all of the diffraction grat
40. lease note that depending on the configuration of your system certain items in the Setup window may be greyed out The LabRAM ARAMIS Setup window is opened by clicking on the Setup button Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific Sramiz Page 242 HORIBA Explore the future HORIBA Scientific 9 11 4 1 Visualization Select Video On or Video Off to control the internal camera optics Video On Optics for internal camera and reflected white light illumination are in the light path Raman path is inactive This mode is used to visualize the sample and acquire white light images of the sample using the internal camera Video Off Raman path is active optics for internal camera and reflected white light illumination are out of the light path This mode is used to acquire a Raman spectrum Select Trino On Trino Off or FTIR in the Top Video section to control the optical path to the optional trinocular head and FTIR module Trino On Microscope eye pieces and top camera are active with illumination beam splitter in place Raman path internal camera optics and FTIR module are inactive Trino Off Raman path and internal camera optics are active microscope eye pieces top camera and FTIR module are inactive FTIR FTIR module is active Raman path and internal camera optics are inactive microscope ey
41. meter Select diffraction grating See Diffraction grating Send to zero order reference position 604 230 Spectral POSITION cccseeecececsesscceeeeseeeeaeseeeesaeeeees 230 Spectrum display sessiccesdsciecosavsineeercrcavataveinea verse See Data Spike filter Automatic spike filter After ACQUISITION ccccseeeeccceceeeeeeeaeeeeeeeeeeeees 126 D ring ACOUISITION sessirnir 37 TUNO nr A 38 TUN Oesman O 38 Manually remove spike after acquisition 166 Split spectral array sshosciseviviclsvaisncecsnniapulideierminesnettones 11 StU Al opera A 199 Active OPOS ezsuaqaswaaceesatunnseeneiatniateiorena teens nentess 199 Cursor WANES siasa nni a ni 201 Detector temperature ssssssssesesesesesrsrrrrsrsesesee 200 Progress baf esssssssesssessserssrrrssrresrereerrserresreseereseee 201 System messages csccesccssccssccsccessceescesscessceseenes 199 Stitch video IMABES cccceeeeeeeeee sees See Extended video Sub pixel prcne See Extended range SUD TRACTS DOCU Aicccsicsahctccusicovsues tees ansienossansnetonendt See Math SWIFT ultra fast mapping See Multidimensional spectral array T Temperature Operating temperature of detector See Detector Spectral profile See Multidimensional spectral array GWM DIGLOS esie See LabAssistant MOST OVC seroren anaE ai 122 Topography Acquire topography image See Extra images Use Autofocus to analyse rough samples See
42. ometers um The tick boxes must be ticked for coordinate read out to be active for each axis Y 130 cape zZ 10 w a Note that if one of the stages is not present for ea example the Z stage then it will be greyed out xo mM yha mE oo Fia Page 236 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA Explore the future HORIBA Scientific The current position coordinates can be set to 0 um by clicking on the set current position as origin icons for each axis To move the stage to a specific position highlight the current value and type in the desired position in micrometers um Press lt enter lt gt The stage will now move to the desired location CAUTION MOVE THE STAGE WITH CAUTION SINCE IT IS POSSIBLE TO DAMAGE THE SAMPLE STAGE OR MICROSCOPE OBJECTIVE IF THE SAMPLE HEIGHT IS NOT CHECKED THERE IS PARTICULAR RISK WHEN THE Z STAGE IS USED WITH HIGH MAGNIFICATION OBJECTIVES The coordinate read out and control can be deactivated for each axis independently by unticking the respective tick box 9 10 2 LabSpec 5 user manual STA Coords ka 200 v Y 130 cl 10 iw ere Coords aZ Coords lt i mal xid Fa IN Fo yha mi zo Mh zko Mi ey Coords ka 200 Y 130 Z 10 Using XYZ Coords with multiple stages The main control of XYZ Coords when multiple stages are configured and active is identical
43. op Video Detection UY Visible CCD PMTVIGA fe UY AIR f Visible Cancel Help f Line Mode Page 244 HORIBA Explore the future HORIBA Scientific select this mode if you wish to analyse bulk materials in the macro chamber using a back scattering geometry Micro In this mode Raman signal which originates from the microscope will be detected You should select this mode if you wish to make standard microscope measurements with the LabRAM ARAMIS External In this mode Raman signal which originates from an external location will be detected using the fiber optical input The light is passed directly from the fiber optical input into the spectrometer bypassing the microscope optics You should select this mode when working with SuperHead fiber optic remote probe heads 9 11 4 4 Measure Select Point Mode or Line Mode to control the optics for standard spot analysis or confocal line scan analysis Point Mode Sets up the measurement for standard spot or point analysis You should select this mode if you wish to make standard measurements with a 0 5 10um laser spot Line Mode Sets up the measurement for confocal line scan analysis You should select this mode in combination with the LineScan scanning mirror controller which allows the laser spot to be rapidly rastored across the sample This is useful for acquiring an average spectrum from along the entire line an
44. orizontally and vertically polarized light By converting the signal from horizontal vertical polarization to circular polarization the difference in spectrometer performance will be removed and all light will be detected in exactly the same way 9 11 3 4 Laser Polarization Select Lambda 2 Lambda 4 or None to insert a polarizor element into the laser beam path to adjust the polarization of the laser beam Lambda 2 or 2 Rotates the laser beam polarization by 90 Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual sploKRA Commutations Adjustment Camera Showy IDETE France Microscope i Trino On i Trino Off Raman Polarization f Horizontal Yertical C Circular i Fiber On Laser Polarization i Lambda Lambda d C None Fiber Off sploRh A Commutations Adjustment Camera Show FiberEntrance Microscope C Trino On i Trino Off naman Polarization f Horizontal Vertical C Circular C Fiber On acer Folanzation f Lambda Lambdar4 None White Lamp e Fiber Off ee Cancel rit sploRh A Commutations Adjustment Camera Show FiberEntrance Microscope C Trino On i Trino Off Raman Polarization f Horizontal Vertical C Circular i Fiber On Laser Polarization Ob Fiber IF f Lambda Lambdar4 None cae HORIBA LabSpec 5 user manual
45. ple shows that there is also a 300gr mm grating which could be exchanged in the B Page 232 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific position This means that it would replace the current 600gr mm grating To replace the diffraction grating first select the diffraction grating you wish to replace and send the spectrometer to Zero Order see section 9 5 page 230 Now take out the current diffraction grating and replace it with desired replacement diffraction grating Once the manual exchange is complete now select the replacement diffraction grating in the My 1800 drop down list 1800 E 300 A message box will appear prompting you to replace the grating Click OK CAUTION THE DIFFRACTION GRATING IS EXTREMELY FRAGILE AND EXPENSIVE ON NO ACCOUNT SHOULD YOU TOUCH OR SCRATCH THE SURFACE OF THE GRATING AS SOON AS IT IS REMOVED FROM THE INSTRUMENT IMMEDIATELY PLACE IT IN ITS PROTECTIVE CASING 9 7 Microscope objective On most systems the microscope is manually controlled and objectives must be manually selected and exchanged However it is important that the correct objective is also selected in LabSpec 5 in order to have correctly scaled video images and for Raman maps to be acquired over the correct part of the sample 9 7 1 1 Selecting a microscope objective The d
46. raction See Baseline correction Baseline correction ssss ssssseresssreressrressreressreressreress 115 Add baseline points manually sssnesseensesssnnssseenn 180 Attach baseline points to data eeeseeseenneee 118 Automatically subtract baseline 116 Convert baseline to Spectrum seecceeseeeeeeeeees 116 Fit baseline ccceeecccccssssecceceeeeceeseeeceeeeeeeeeeseaneees 116 ET Ws eee rcenpet esas eect on aneussiems tans eeeeanavountonss 117 Remove baseline points manually cccseeeees 181 Setting a baseline For a multidimensional spectral array 120 For a single Spectrum cccccccsseeceeeeceeeeeeeeesees 118 Subtract DaSeliNE ccccseeeecccceeeeeeeeeeeeeeeeaeeeeeesaees 116 Baseline points Adjust baseline points manually See Baseline correction Page 251 Medical Semiconductor Scientific HORIBA Explore the future HORIBA Scientific Batch PrOCOSSING sicctsssadsaccssceatcavaidviavasssnteasedearase See Multi BE CO Miva teas exer nereasetaie nese Gowers enna daaseuvaasecade See Icon bar Binning factor Detector image real time display RTD 33 Spectrum ACQUISITION ccceeccceececeeseeeeceeeseeeeeeeeees 36 C Calibration Instrument MOtOlSsrrisrrrseisossssoissesinrsnrinieeid ienien 74 Internal alignment diode cccccccsssseeceeeeeeeeeeeees 74 Manual procedure for LabRAM system 247
47. rence 20027 50 Counts 2nd Spectrum 160 45 es 3rd Spectrum f Be 4th Spectrum pf j x Pixel Calibration Set Unit to Pixel Show Diode Show Central Pix M Open Shutter Motor Calibration Motor Spectro X Position 654360 inf Grating 1800 Zero 8000 Apply Koeff 0 002526 Cancel Help Spectrometer aki 1o96 om Page 248 HORIBA Explore the future HORIBA Scientific 10 2 Calibration of Raman Peak KOEFF Move the spectrometer to 520 7 cm to analyse the silicon Si first order Raman peak Acquire a spectrum of the silicon Si sample using the real time display RTD function Ensure the detector is not saturated if the signal is too intense insert a filter in the laser path reduce the confocal hole diameter or decrease the acquisition time Stop the spectrum readout by clicking STOP Zoom in on the peak using the Zoom icon in the Graphical Manipulation toolbar Use the cursor to locate the center of the 520 7 cm silicon Si peak If the peak is within 1 data point of 520 7 cm the calibration is acceptable If the peak is at a position greater than 1 data point from 520 7 cm the calibration must be adjusted To do this click on Setup gt Instrument Calibration to open the Calibration dialog window Adjust the KOEFF parameter and then click Apply You should see the peak move Continue to adjust the KOEFF value until the band is within 1 data
48. rization i Fiber Off Lambda Lambda 4 None SS Cancel Init Page 241 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA ww Scientific 9 11 3 7 Init The Init button will re initialize all of the XploRA motors and confirm that they are at their correct AploRA Commutations Microscope Adjustment Camera positions C TrinoOn 9 Trino Off Show Ramar Polarization FiberEntrance Horizontal Vertical t Circular z Fha Tr m rae Ge ae Fiber Diff Lambda Lambda d None White Lamp 9 11 4 Control of Hardware Options for LabRAM ARAMIS The LabRAM ARAMIS Setup window allows control of the following optional items Measurement location microscope fibre optic probe macro chamber Laser and Raman path polarizors Trinocular head on the microscope Multiple detectors Point Mode and Line Mode optics UV IR and visible dual path optics O O O O O Measure Location Polarization Laser C Vertical f Horizontal f Circular C Macro 30 C Retro f Micro C External r isualization f VYideoOn C Video Off f Tino On Trno Of E FTIR Raman C Vertical C Horizontal f None l Scrambler Measure i Point Mode f Line Mode Top Video M Detection CCD C PMT IGA UM Wisible w UY IR C Visible Cancel Help P
49. rocess amp Environmental Medical Semiconductor Scientific HORIBA HORIBA Tes Scientific o Y graded fill from low Y axis value to high Y axis value 100 100 o Z graded fill from low Z intensity axis to high Z intensity axis 100 100 Color Select the line color from the Color drop down box Note that the Line and Width options in this drop down box are not active for 3D images Fill Select the fill color from the Fill drop down box The fill color selected in the Fill drop down box is only displayed when the Line and Fill type is selected and no gradation option is selected see above If a gradation option is selected the fill color is set according to the palette selected in the Colors dialog window see section 8 6 page 210 Note that the Fill option in this drop down box is not active for 3D images Available for Video Solm Map Score when displayed in 3D mode Page 226 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA LabSpec 5 user manual HORIBA Scientific 9 Control Panel The Control Panel located at the bottom of the screen contains sections which are directly related to the hardware configuration of the instrument This panel will only show sections for devices which are correctly installed and configured The description below shows all of the standard sections but
50. rop down box lists all of the objectives available on the microscope Ww 1800 gt heo Click on the drop down box to display the list and 9 100 F am then select the objective to be used w sample A Es Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA HORIBA malian Scientific A message will prompt you to now select the desired objective Once the correct objective is selected on the microscope click OK 9 8 Data name tag The data name tag is a prefix which is used for every newly acquired spectrum In addition it is used as the default file name during a File gt Save As procedure If the data name is unchanged for multiple accumulations each new spectrum will be acquired with the same prefix and then sequentially numbered In the example shown to the right the data name tag has been set as sample_A Each spectrum will My be acquired as sample_A_1 sample_A_2etc y a l x100 Re If the data name tag is left blank as in this example then spectra will be automatically Mn labelled as 7 2etc F jac he 9 9 Acquisition The Acquisition section allows the user to specify acquisition times also called exposure times for the Real Time Display Spectrum Acquisition and Mapping Acquisition and the number of accumulations or averages for each measurement Acquisition lt b i Real Time Display RTD
51. ssenvensesenscasnesaceanwerssatwuseaneavaeere 147 DVN ee i PEE E PETEN AI AA E A AT 130 Modelling sanvessiincnavvcivanndapvavtis easvandanendunvextoaxbunved 154 Peak searching and fitting ccccsseeeceeseeeees 134 POTE a ie eee dees ete 145 SMOOTHING cseesccccesssseccecaeeeeceeaeeeeesaeeeeeeseees 124 Spectral ID SCArCh ccccccsssseeececeeeeeeeeeeeeeeeaees 114 Spectral normalisation and correction 120 Delete data iCOM cccccsseeccceccessececeeeeceesaeeeceesaeeeeees 90 DETE vate seta ncn cecas tees N 90 SM all CO scis 89 Stop active FUNCTION ICON ceeeccecceeeseeeeeeeeeeeeees 163 Stop active FUNCTION cccceeeeccceceeeeeeeeeeeeeeeeees 163 Switch between Big and Small icons 06 89 Image Color pel ES aa 211 Contrast and brightness cccccccseessseceeeeeeeeeeees 212 Overlay map score information on video image 222 SCANS OW aa E acicutnstentepedennensicrnsneda 222 SIMOOU MOS serverer in ninr o a See Smoothing Information Custom information ccccceeeeeeeees See Custom info Parameters waccenceasreomacnvsidineesannsetvxcureanceverramedneceee 92 Ae TO a 94 Ea E EA A A ATS 94 FUS WOOK oreore Er EEE EIE 95 Intensity correction s sssessesssesssessssseresesserssessereseesse 43 Apply correction after aCquisition cccccceeseeeees 45 Automatic correction during acquisition TOT O 9 peer eee nero nitee ie Mir nn ESR r
52. ssseeeeeees 91 CONUS CUNSONS sajiccnesscaersarnecorvearianeviymtaxenmniesqenees 92 Aa WAIN C ce desea xndenc partons evetvacntaseussabesatnncesetotassacs 92 Intensity normalization ccceeeseeeeceeeeeeeeeeeees 92 Scale normalization cccccceesseeececeeeeceeeeceeeeees 91 Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual Data ACQUISITION ICONS c cecccseceeeeeceeeeeeceseeeeneees 95 LUOS NN a E AE E EA 110 Extended range acquiSition cccccsssseeeeeeeeees 102 Extended VIGGO ccccccccssseeeceeeesseeeeeseeeeaeaeees 102 Mapping ACQUISITION ccccseccceeecceeeeeeceeeeeeees 96 Mapping Properties ccccccssecccsecccsecceeeeneeeeeees 97 Multi P OUT tsssccssacirastentisadseateaemedanerdrateteapatdeds 104 Spectrum ACQUISITION ccecccseecccesceeeeceeeeeeeeeees 96 Spectrum RTD real time display cc ceeeee 95 NG EIE E EE E E EN E E E 102 Data management ICONS ss ssssssssssesssersreserssersrresee 90 B EEE AEE E inte EE EA TT 91 Biel AEE A T A A adic tap aan 90 PUR E E E E A E E 91 DAY EON ET EE TE EENE E T ETE 91 Data processing and analysis iCONS cccceeeeeee 114 Baseline COL CtION cc csseeccceceesseceeseeeeeeeeeeees 115 Create spectral profile cccccccesesceeeeseeeeeeeees 149 Fourier transform ccccccsssecccceeeeeeeeseeeeeeseesees 127 Map ANGIVSIS i cersesc
53. sssssesssesserssessreserssesserssees 235 Number of acCuUMUIatiOns sceecceeesseeeeeeees 235 Real time display RTD See Real time display RTD Start ACQUISITION scccsesenscancsenaisncpaadeveitenaiseosibalencses 96 Video Start acquisition recenser tiren EA 102 Start extended video image acquisition 104 ACQUISITION MENU ssscscearaodeasencedcrsonsedtecwsseceanadsenseanndeasayaes 30 AUTOFOCUS soscccasssnocicosaeeawansatasiasaanatinseduesitasnmnisdastnesacas 59 CUSTOM INFO ccceeeecccceeseseceeceeeeeeecaeeeeseaeeeeeesaeeeeees 30 SCC CUON enea E AAA 57 Extended range urere oe OEE R 49 Extia IWS OS ca E EE A 63 Heat COSCO rasen 66 DDOE arer a O A O T 34 PEO aa E ae vee ne 33 AUNTS SOY ona a E AE 32 Acquisition parameters Accumulation MOCE ccseeececesesseceeceeeeeeeeeeeeeesaees 37 Acquire spectrum with pixel units for X axiS 73 ACQUISITION TIME ccccceeccesecceecceeecesecseeessesenaess 235 AUTO CXPOSULE cccseccssceseceeeeees See Auto exposure AUTO SAVE a ceeotrraxtateveiseuaren wattenaenoaseenaniie See Auto save PUUTOTOCUS oreren annene EO ENEO See Autofocus DEDOS O er A E ate ee 46 Intensity correction See Intensity correction Mapping See Multidimensional spectral array Multidimensional spectral array See Multidimensional spectral array Number of acCUMUIAatiONnS cccccsseseeceeeeeeeeeeees 235 Photo bleaching time See Photo bl
54. te tear are Re nee 45 TO O inna E E A 44 How is the correction factor calculated 666 44 Select a filter nicecicsectncecavannessedeseunacpeaansteadtaueettarecsaenens 75 Page 254 HORIBA Explore the future HORIBA Scientific Status ar INGICALON icsiscaciesiisentevinedacdeisiacdsvinctcianiisas 199 Use with manually selected filters ccceccceeeeeees 75 Intensity PrOPFile ce ceccccssssecceceeeceeseeseceeeeeeeeesseeees 145 L LabAssistant TS SS anr 76 BEE essere ccass eshctasotaesieceienraesseroust AE AAA TA 78 CUSTO o o E E O E teaates 77 DET a EEE 76 Display templates in the Custom list 06 79 OVE INISINE serari aE ES 79 DA a E EEOAE A A A E 78 LabAssistant menu Create template eserssriseerereirrrrsererersarrissereressrersens 78 Custom templates cecccccsseecceceeeesececeeeeeeeeeeeeeeees 77 Default templates seienrinprriseren nnna 76 G O Guided Operation Wizard cccccceeeeeeeees 82 Manage templates cccssscccccssseeceeeeeeeeeeeeeeseeseees 79 LAID MOG T EE E EEPE E E 21 Label peaks ccccceeeee See Peak searching and fitting LabRAM ARAMIS Hardware CONO i sascaivesoncaadceanvenseaseceawssorwiuvevencaives 242 Measurement location cccccccsseeeeeeeeeeeeeeeees 244 Measurement mode point line 0086 245 POA Za LOU sicrie 244 Select detector snra 246 Select Visible or UV IR optical path 0 246 V
55. ting A CAMEL A ccccssseccccaesseecceeeeeceseuaeeeesseasees 72 Set the image scale cccessccccsssseeceeeeeeeeeeeeeeeeeaees 68 Manual ser sautteanactiansneticaseneecscrienspartetensseigneeannds 70 SUN ara E aes 68 Set the laser spot marker position cseeeeeeee ees 67 Set the LineScan image scale ceesccecsseeeeeeeeeeees 71 Start ACQUISITION ss cicaseosecvereessaeaacawersaswiyeavsnensentereemanens 102 Video MENU niei 66 FOIA ecran a ES ANRA 67 Laser POSITION eccessi crror Enr EATEN 67 List of installed CAMELAS ccccccssseseeeceeeeeeeeaeeeeeees 72 OSE aaan EETA 67 SOU CO rA E E E EEEN OT OE 67 Video image scale ccseeeccccsessseeceeeeseeesaeeeeeeseeeeees 68 Visual basic scripting iiccssssesscusssardecsoasverseesaevancen See Scripts W Warm detector to room temperature cecceeeeeeeees 66 Window MENU si ccscnsasscoeseaescicessseeanetasutsaoudasatebseavatdessasere 86 C 6 Renee ee me eee eer ener ae cee ener t 87 Page 258 HORIBA LabSpec 5 user manual HORIBA Scientific TIS E ES PEIES AIIE EI TE PE OET E E 87 INSTFUMENT SETUP sisisi senais ieni 238 Turn On lase cenin 238 X Y X axis Reverse display ceecccccsssecceeceeeeeeeseeeeeeseeeeeeesees 209 Y axis CAINS EN E E E EE E EE OEA E T 23 Reverse display a sssaacsstncnssadetvacesbaxvavenatdeaeaenisnnatinnes 210 XploRA AE ees T seas atenas 23 Hardware COMO sisscctcssassniet vasavacetntvensiwacsetaneassanabee
56. tion ssisccasssessiarscersadesravsisseneersesesesens 133 During ACQUISITION sssssssessessessrrssesrrssersrrssee 55 Adjust intensity ccise cai cseseconressecpasoaveavaserunnensessucenvennucas 55 Combine data acc case tacticacsantoraciccsconestaceca etestaencsaccettaunss 54 Combine individual windows Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific LabSpec 5 user manual After ACQUISITION ccccccessssecceceeeececeeeeeeesaeeees 133 During acquisition v ssocccscsvecsnnvescsssdasssedavedeveressnaves 54 EO E AE E E A A tea amners 102 Modes of operation sesssesssssereessseeesssressreressreressne 50 Status bar indicator cceccccccssssecceeeeeeeeaeeeeeeseees 200 SU od Ee 52 APCD SOUT sanean 56 TIA ONTE EE EEE EO EEO O E ANON 56 Extended VIGO crcire a NA 102 PRC CIM a E E E E A A A E 104 Extra NAG CS eaen rr ENT E TONOA 63 ACGUING an MARC seronsisnicroniisiro en 64 Acquire an image during a map acquisition 64 What are they TOM sccscicevacsoveneethesaswenteavavesiciesverendanses 63 ECL Ne estes sec tissiercttuz cut cu teosaics EEA 21 F Factor ANALYSIS csconwecciccedeneteanesanecentbnscsunecsesk See Modelling Fiber entrance ADIO A ner aE ONE 241 File TOMA e E E E E 10 File Menu ccccceeeeccccsesssecceceeseceeseeceesueeeessaeeeeesseeseeeeees 10 TSC ANE E O E E A OE E T A 11 B3 APTE A E N EA T A TAT 11 PE A esr EAE E EATE O E O ON 13 PUI
57. to that outlined above see section 9 10 1 page 236 Note that the name of the XYZ stage is displayed in this section typically the stages will be XYZ Motorized for a standard motorized stage and XYZ Scanlab for the DuoScan stage see section 4 5 10 page 110 Click on the switch stage icon to select the desired stage Aulomotive Test Systems Process amp Environmental Medical Semiconductor Scientific ee Motorized Yz Scanlab x 0 00 i x 0 00 i y foo WE jy foo mH z 0 00 z 0 00 mo Page 237 HORIBA HORIBA ws Scientific 9 11 Instrument Setup ploRA Aramis L d LCa Setup The Instrument Setup section is only present for the XploRA or LabRAM ARAMIS systems It allows control of the laser beam on off and other hardware options such as polarizors trinocular head macro chamber etc 9 11 1 Control of Laser Beam for XploRA On the XploRA system the selected laser see section 9 1 page 227 can be turned on or off using the laser toggle switch Setup it Click on the toggle switch to turn the laser on and ploRA ploR A off HL HE Laser off Laser on Setup 9 11 2 Control of Laser Beam for LabRAM ARAMIS On the LabRAM ARAMIS system the mechanical shutter for the selected laser see section 9 1 page 227 can be opened or closed using the toggle switch When the shutter is open the sample will be exposed to the las
58. ual calibration values for each diffraction grating A full calibration of a system with multiple lasers requires one of the lasers to be designated as the reference laser the wavelength of this laser is assumed to be constant and known The remaining laser wavelengths are then calibrated relative to the reference laser Before performing a manual calibration ensure that all lasers which are to be used for the calibration have been turned on and left to warm up for a minimum of 15 30 minutes This is essential to ensure the laser wavelength has stabilised 10 1 Calibration of Zero Order Position ZERO Insert the silicon Si calibration sample underneath the microscope and focus on it in the normal way using a 50x or 100x magnification objective Selecting the diffraction grating you wish to calibrate using the Diffraction Grating drop down My 1800 box in the control panel a0 Te Select the reference laser from the Laser drop P down box in the Control Panel d 632 817 n Send the spectrograph to zero order 0 nm using Spectrometer the icon in the Spectrometer section of the hardware toolbar M i 1073 95 E Page 247 Explore the future Automotive Test Systems Process amp Environmental Medical Semiconductor Scientific HORIBA Explore the future HORIBA Turn on the reference laser and acquire a spectrum at zero order 0 nm using the real time display RTD function Ensure the
59. uctor Scientific LabSpec 5 user manual Ri cre S O aaerem een e ett ether Conrernt race E 220 FR gags espa Geese acta ee 209 SC ANS 0 A o E EEA E TE 222 SPEE ee aE E 217 Swap X AXIS anssyeveanacvessanedensunsatosmaneathucaenesendseesounesanes 209 SWAN AKE arrie EE 210 RED oaeee See Real time display RTD S Save Options for saving data ccccsseeecceeceeeeeeeeeeeeeeseees 12 SEI alll Ca lel PE E E E T 11 Save all spectra in array as individual files 11 E SS E IE E EEE S EAT TE E 11 Save 6 4 sarc cera aA an nnn er 12 SaVvitSky Golay ccccssssecceceseseeeeeeeeeeeeeees See Smoothing Scale Format scale D EHAVIOUL ccccccsseeeeeeeeeeeeeeaeeeees 208 Rescale PV CS aea a arenes tenants EA R Giese 91 Drag spectrum with mouse to rescale 169 Intensity cssasssetsnasseensseecavonbareindneanegiexiucdeoeseneemeseetess 92 Drag spectrum with mouse to rescale 169 Scale bar fOr images secccccsssseeeceeeeeceeeaeeeeeeseees 222 Scale data manually shift stretch compress axis 21 Set scale of video images 666 See Video images CNE sasesawacanesueatunseuccaeuunseus oun cenaiieseacosas See LineScan S ae T E A E OA E AE E 82 Add scripts to menu list ceecccecsseeeeceeeeeeeeeeeeeees 85 Apply a script before after acquisition 0 85 BU A ni bitte occ vc ns detan dv A NEEE OAE 84 Load and Manage arscsspesscespaardeestasicasnnavaceussteiee
60. usenaenas 83 Memu Hst sccicascccsseccncessanctecsesasesdvessanodedaeesesstavenddsvaeees 86 Remove scripts from menu list ccccceeeeeeeeeeeeeees 85 NE SLOW arane E 85 Use scripts to control MOTOTS sesssessssrsereresrererere 86 Sonts men U ea aE ENE 82 COONS ae E 83 Se FO DAS A EEEE A E E T 207 SECU Me MU dia taeresemaenatntasis 73 CS E S EE ET A ET AE E ET 75 Instrument calibration s sesssesssssenseesserssrrresssrrssse 73 istrument Mit sacarrencerd teense a 75 SUE E seq deeccueecianesee seeeceenee 229 Small COM sasssncicassansasavssecncssasadeessssansearoaesscasaee See Icon bar Smoothing Data Denoise After ACQUISITION cccsseeccceceeeeeeeeeaeeeeeeaees 124 Automatic Denoise during acquisition 46 Page 257 HORIBA Explore the future HORIBA Scientific TONON aaa NA 47 MOEN O eea A A 47 Fourier transform ccccsseecccceeeeeceeceeesceeseeeeeeees 127 Mediaren EE 126 Savitsky Golay ccccsssscccccssscecccceseececeseeeesseeeees 125 Image pixel SMOOTHING ccccsseseecceeeeeeeseeeeeeees 221 Spectral database cccccssseeceeeeeeeeeeeeees See Spectral ID Spectral ID Configuring LabSpec correctly 114 Start search from LabSpec cccseseeeeeeseeeeeeeeeees 114 USING cccaciorsecsoasiveriins See separate Spectral ID manual Spectral profile See Multidimensional spectral array Spectral range ccccccsssccccsssseeeeeeeees See Extended range Spectro
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