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Thermal Cycler Temperature Verification System User Guide

1. Data Sheet Calibration Verification Test Data Sheet Temperature Non Uniformity Test Appendix A Recalibration Obtaining RecalibranlOn 3 dre GU d Ret eee nae ha E ER ke oh Ut Appendix B Instrument Warranty Information Computer Configuration lese rs Limited Prod ct Waranty 35329 riti WUS wur ac ORO CAR d ODORE p d d Cn Damages Claims and Returns Appendix C Certificate of Decontamination Index IV Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Preface How to Use This Guide Purpose of This The Applied Biosystems Thermal Cycler Temperature Verification System User Guide Guide provides information for using the Thermal Cycler Temperature Verification System with GeneAmp PCR Systems with a 0 2 mL Sample Block It describes the specific tests for the various thermal cyclers with which it 1s used Audience This guide is intended for novice and experienced GeneAmp PCR System users Text Conventions This guide uses the following conventions Bold indicates user action For example Type 0 then press Enter for each of the remaining fields Italic text indicates new or important words and is also used for emphasis For example Before analyzing always prepare fresh matrix e A right arrow bracket gt
2. 5 8 Data Sheet Calibration Verification Test 5 13 Data Sheet Temperature Non Uniformity Test 5 14 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 1 Chapter 5 Temperature Verification Tests for the System 2400 Overview Materials When you perform temperature verification tests on the System 2400 you use the materials provided in your kit Assembly Figure 5 1 shows a properly assembled system Note that the frame is positioned around the wells with the channel facing you and the probe wire 1s threaded through the channel to prevent damaging the wire when the thermal cycler s heated cover is closed Figure 5 1 Proper assembly of the Temperature Verification System for System 2400 5 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Test Overview Use this test to verify the temperature calibration of your system 2400 The Calibration Verification Test consists of the following subprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 5 3 Configuring the System 2400 5 4 Running the Test 5 4 Evaluating the Results gt Ending the Test 5 7 ANETE PHYSICAL INJURY HAZARD Hot Surface Use care when worki
3. Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 5 8 Configuring the System 2400 5 9 Running the Test 5 9 Evaluating the Results 5 12 Ending the Test 5 12 ANETE PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components This test requires the 0 2 mL Temperature Verification Kit PN 4317939 which includes Cotton swabs Light mineral oil 2400 Temperature Verification Frame 0 2 mL Probe Assembly Model 4500 digital thermometer with 9V battery installed To set up the 0 2 mL Probe Assembly l If the heated cover is in the forward position lift the lever then slide the heated cover back Place the 2400 Temperature Verification Frame on the sample block Using a cotton swab coat the following wells with mineral oil A2 A7 B1 B4 B8 C2 and C7 Place the 0 2 mL Probe Assembly into well A2 Note As the test progresses you move the 0 2 mL Probe Assembly to each of the test wells Thread the probe wire through the channel in the 2400 Temperature Verification Frame to prevent damage to the probe and lead wires See Figure 5 1 on page 5 2 Make sure the probe is connected to the digital thermometer 5 8 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test 7 Slide the heated cover forward and pull the lever
4. 4 12 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 1 Chapter 4 Temperature Verification Tests for the System 9600 Overview Materials When you perform temperature verification tests on the System 9600 you use the materials provided in your kit Assembly Figure 4 1 shows a properly assembled system Note that the frame is positioned around the wells with the channel facing you and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler s heated cover is closed Figure 4 1 Proper assembly of the Temperature Verification System for System 9600 4 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Test Overview Use this test to verify the temperature calibration of your 9600 system Required Materials Setting Up the 0 2 mL Probe Assembly The Calibration Verification Test consists of several subprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 4 3 Running the Test 4 4 Calculating Test Results 4 6 Ending the Test 4 7 AS erence PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components This test requires the 0 2 mL Temperature Verifica
5. Chemical Safety To minimize the hazards of chemicals Guidelines Read and understand the Material Safety Data Sheets MSDS provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials See About MSDSs on page xii Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines consult the MSDS Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the MSDS Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended on the MSDS Comply with all local state provincial or national laws and regulations related to chemical storage handling and disposal Electrical Safety MAMMA ELECTRICAL SHOCK HAZARD Severe electrical shock can result from operating the Thermal Cycler Temperature Verification System without its instrument panels in place Do not remove instrument panels High voltage contacts are exposed when instrument panels are removed from the instrument Fuses AMEL ELECTRICAL SHOCK HAZARD Improper fuses or high voltage supply can damage the instrument wiring system and cause a fire Before turning on the instrument veri
6. Sending To send your equipment for recalibration Equipment to 4 Alpha Technics Inform Alpha Technics of the recalibration order by either of the following methods Mail the purchase order directly to Alpha Technics 1560 Orangethorpe Way Anaheim CA 92801 Or Faxthe purchase order directly to Alpha Technics 714 773 9327 Ensure that the probe assembly has been decontaminated Pack the Model 4500 digital thermometer and 0 2 mL Probe Assembly in the black case then send to the address above A 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Obtaining Recalibration 4 Create a package with Black case containing digital thermometer and probe assembly Decontamination certificate see Appendix C Payment of 135 00 plus 15 00 for shipping handling purchase order or company letterhead with the words verbal purchase order or e Visa MasterCard credit card information Address and contact information billing address shipping address name and phone number of a contact person most familiar with the thermometer Note If payment is not included with the package Alpha Technics bills you for the amount of 150 00 plus a 10 administrative fee The total for the purchase order is then 165 00 5 Send the package freight prepaid to Alpha Technics at the address shown in step 1 Thermal Cycler Temperature Verification System fo
7. See Figure 3 1 on page 3 2 6 Make sure the probe is connected to the digital thermometer 7 For the 9700 System with manual Dual 96 Well Sample Block Module Slide the heated cover forward and pull the lever down For the 9700 System with Auto Lid Dual 96 Well Sample Block Module Press the red button to slide and close the heated cover IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Turn on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermomete Configuring the To configure the 9700 system System 9700 1 Start up the 9700 system The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The Diagnostics screen appears 4 Press F3 TempVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU Exit F1 F2 F3 F4 F5 5 Press F2 TNU The 9700 system is automatically configured for the Temperature Non Uniformity Test starting with the setpoint of 95 C and the TNU Performance screen appears The following screen appears when the cover reaches the setpoint TNU Performance Sample temp xx x C Cover temp xxx C P
8. L labels instrument safety x laser hazard symbol x M menu commands conventions for describing v moving and lifting computer xi monitor xi moving and lifting instrument safety xi moving parts hazard xiv hazard symbol x safety xiv symbol xiv MSDSs description xii obtaining xii referring to xiii whentoreview xi MSDSs obtaining vi N Notes description v O overvoltage category rating xiii overvoltage rating xiii P physical hazard safety xiv physical hazards xiv pressurized fluids hazards xiv safety xiv See also compressed gases probe assembly 1 5 H RA number B 4 RA number See return authorization number recalibration 1 4 A 1 to A 3 reporting damages B 4 return authorization RA number B 4 returning damaged items B 4 returns B 4 S safety before operating the instrument xi chemical xii compressed gases xiv conventions vili electrical xiii instrument xi instrument operation xi moving and lifting instrument xi moving parts xiv physical hazard xiv solvents xiv standards xiv safety alert words accompanying hazard icons viii CAUTIONS viii DANGERS viii description viii IMPORTANTS viii WARNINGS viii safety labels on instruments x safety standards Canadian xiv European xv U S xiv safety symbols oninstruments ix See also hazard symbols Services and Support obtaining vi Index 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL
9. 2 3 Temperature Non Uniformity Test 2 8 Data Sheet Calibration Verification Test 2 13 Data Sheet Temperature Non Uniformity Test 2 14 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide lii Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module SUCI eera nana a r T Aa a en sn Sate ee a eae ee ees Calibration Verification Test Temperature Non Uniformity Test Data Sheet Calibration Verification Test Data Sheet Temperature Non Uniformity Test Chapter 4 Temperature Verification Tests for the System 9600 OVerVIOW 4 sd te qi OR dod Ded deed x UR a Ded A Le oda do MEC e Eod ACD LAE e ou Calibration Verification Test Temperature Non Uniformity Test Data Sheet Calibration Verification Test Data Sheet Temperature Non Uniformity Test Chapter 5 Temperature Verification Tests for the System 2400 OVEN dc deans pane ee dad D ees Daci PAWS Rede due A alo dux oo Calibration Verification Test Temperature Non Uniformity Test
10. ISM Radio frequency Equipment Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide XV Safety and EMC Compliance Information XVI Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Introduction This chapter covers About his vanilla ies vtto TODA PSI OB dp IA M do bus 1 2 About the Temperature Verification System 1 2 0 2 ml Probe Assembly isa dnd Kh Sal ea e ERRORES EEG ERR E do od 1 5 Digital h MOMENT oe E d Pre pedo Ee v SE 1 5 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 1 1 Chapter 1 Introduction About This Manual This manual describes the Temperature Verification System and the tests for the thermal cyclers with which it is used GeneAmp PCR System 9700 96 Well Sample Block Module GeneAmp PCR System 2700 GeneAmp PCR System 9700 Dual 96 Well Sample Block Module GeneAmp PCR System 9600 GeneAmp PCR System 2400 This manual contains a separate chapter for each instrument except for the system 9700 and system 2700 which share the same instructions Each instrument chapter provides step by step instructions for performing two different tests which are described in Temperature Verification Tests on page 1 4 This manual is included in the kit the Temperature Verification System P N 43179
11. to keep a permanent record of the test Press Enter The system 2400 automatically begins the second reading 37 C setpoint and TNU Performance screen appears with the setpoint value displayed 5 10 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 37 C Sample must be within 1 0 C of setpoint Cancel F1 F2 F3 F4 F5 Note The sample block must be at 34 C 1 0 C 5 Repeat steps 2 through 4 for the second reading 6 Press Enter The TNU Performance screen appears with the following prompt TNU Performance Sample temp xx x C Cover temp xxx C Place probe in well xx Press Run Run Cancel F1 F2 F3 FA F5 7 Slide the heated cover back and repeat steps 4 through 7 of Setting Up the 0 2 mL Probe Assembly on page 5 8 and steps 1 through 6 of this procedure Complete these steps for the remaining 6 wells to be tested A7 B1 B4 B8 C2 and C7 8 The system 2400 evaluates the uniformity of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test 379 C XX X XX X XX X Cancel F1 F2 F3 FA F5 If you entered values on the Temperature Non Uniformity Test Data Sheet page 5 14 compare those values w
12. 37 C A2 A7 B1 B4 B8 C2 C7 5 14 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Hecalibration This appendix describes how to obtain recalibration service for your Temperature Verification System Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide A 1 Appendix A Recalibration Obtaining Recalibration Introduction Applied Biosystems recommends that the Model 4500 digital thermometer and 0 2 mL Probe Assembly be recalibrated on an annual basis To have your thermometer and probe recalibrated l Decontaminate the probe assembly as described below 2 Send the equipment to the manufacturer as described below Decontaminating To decontaminate the probe assembly the Probe Assembly l Gently wipe the probe cone with a cotton swab that has been moistened with bleach solution 20 bleach 80 water IMPORTANT Do not submerge the probe in bleach solution Do not disassemble the probe assembly CHEMICAL HAZARD Sodium hypochlorite bleach is a liquid disinfectant that can be corrosive to the skin and can cause skin depigmentation Please read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves Complete a Decontamination Certificate See Appendix C Certificate of Decontamination
13. C of 85 C Cancel F1 F2 Bo F4 F5 Note The cover must be at 105 C 1 C It may take several minutes for the system 2400 to ramp up The Calibration Verification screen counts down the time until the setpoint is reached Calibration Verification Block temp xx x C Cover temp xex C Stabilizing at setpoint Deae Cancel F1 F2 F3 FA F5 2 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer 3 Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field Calibration Verification Block temp xx x C Cover temp XxXxXX C Enter actual block temperature Cancel F1 F2 BS FA Fb5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the Calibration Verification screen Note Record this value on the Calibration Verification Test Data Sheet page 5 13 to keep a permanent record of the test 4 Press Enter The system 2400 automatically begins the second reading 45 C setpoint and the Calibration Verification screen appears with the setpoint value displayed Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 5 Chapter
14. Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide vii Safety and EMC Compliance Information Safety Conventions Used in This Document Safety Alert Words Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards Each alert word IMPORTANT CAUTION WARNING DANGER implies a particular level of observation or action as defined below Definitions IMPORTANT Indicates information that is necessary for proper instrument operation accurate chemistry kit use or safe use of a chemical N eie Indicates a potentially hazardous situation that if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices ANITY ndicates a potentially hazardous situation that 1f not avoided could result in death or serious injury N PYiicldid Indicates an imminently hazardous situation that if not avoided will result in death or serious injury This signal word is to be limited to the most extreme situations Except for IMPORTANTS each safety alert word in an Applied Biosystems document appears with an open triangle figure that contains a hazard symbol These hazard symbols are identical to the hazard icons that are affixed to Applied Biosystems instruments see Safety Symbols on page ix Examples The following examples show the use of saf
15. Diagnostics screen appears 4 Press F3 TempVer The Temperature Verification screen appears Temperature Verification Cover Setpoint is 105 C Cover must be within 1 0 C of setpoint Cancel F1 F2 F3 FA F5 5 Press F2 TNU The 9700 2700 system is automatically configured for the Temperature Non Uniformity Test starting with the setpoint of 37 C Then the TNU Performance screen appears TNU Performance Sample temp xx x C Cover temp xxx C Place Probe in well Al Press Run Run Cancel Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 9 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Running the Test The Temperature Non Uniformity Test uses the 0 2 mL Probe Assembly to test the temperature uniformity of 8 different wells in the sample block Note Press F5 Cancel if you want to exit the test To run the Temperature Non Uniformity Test 1 Press F1 Run The Temperature Non Uniformity Test starts and the TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 37 C Sample must be within 1 0 C of setpoint Cancel EL F2 F3 F4 F5 Note The sample block must be at 37 C 1 0 C The TNU Performance screen counts down the time until the setpoint is stabilized TNU Performance Sam
16. Kit for details on operating the Model 4500 digital thermometer Configuring the To configure the system 2400 System 2400 Start up the system 2400 The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The Diagnostics screen appears 4 Press F3 TmpVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU ecc F1 F2 F3 F4 F5 5 Press F1 Temp The system 2400 1s automatically configured for the Calibration Verification Test and the Calibration Verification screen appears Calibration Verification Block temp xx x C Cover temp xxx C Place probe in well B4 Press Run Run Cancel F1 F2 F3 FA Fb5 Running the Test In this test you take temperature readings of the sample well at two different setpoint temperatures using the 0 2 mL Probe Assembly Note Press F5 Cancel if you want to exit the test To run the Calibration Verification Test 1 Press F1 Run The Calibration Verification Test starts and the Calibration Verification screen appears with the setpoint value displayed 5 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Block temp sx x C Cover temp e xc Setpoint is 859 C Cover must be within 10
17. Sample Block User Guide solvents safety xiv standards EMC xiv safety xiv symbols hazard ix hotsurface ix moving parts xiv symbols on instruments electrical ix safety ix systems covered 1 2 T Technical Communications contacting vi e mail address vi Technical Support contacting vi technical support for computers with altered configuration B 2 Temperature Non Uniformity Test system 2400 5 8 to 5 12 system 2700 2 8 to 2 12 system 9600 4 8 to 4 10 system 9700 2 8 to 2 12 system 9700 with dual 96 well block 3 8 to 3 12 Temperature Verification System 1 2 to 1 4 tests introduced 1 4 See also Calibration Verification Test and Temperature Non Uniformity Test training obtaining information about vi U US safety standards xiv user attention words defined v W WARNING description viii warranty damages claims returns B 4 exceptions B 3 for computers with altered configuration B 2 limitations B 3 period B 2 warranty claims B 3 watranty exceptions B 3 warranty period effective date B 3 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Index 3 Index 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 Worldwide Sales a
18. TRADE ALL OF WHICH ARE EXPRESSLY DISCLAIMED Warranty THE REMEDIES PROVIDED HEREIN ARE THE BUYER S SOLE AND Limitations EXCLUSIVE REMEDIES WITHOUT LIMITING THE GENERALITY OF THE FOREGOING IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE INCLUDING WITHOUT LIMITATION ANY TRADE PRACTICE UNFAIR COMPETITION OR OTHER STATUTE OF SIMILAR IMPORT OR ON ANY OTHER BASIS FOR DIRECT INDIRECT PUNITIVE INCIDENTAL MULTIPLE CONSEQUENTIAL OR SPECIAL DAMAGES SUSTAINED BY THE BUYER OR ANY OTHER PERSON OR ENTITY WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES INCLUDING WITHOUT LIMITATION DAMAGES ARISING FROM OR RELATED TO LOSS OF USE LOSS OF DATA FAILURE OR INTERRUPTION IN THE OPERATION OF ANY EQUIPMENT OR SOFTWARE DELAY IN REPAIR OR REPLACEMENT OR FOR LOSS OF REVENUE OR PROFITS LOSS OF GOOD WILL LOSS OF BUSINESS OR OTHER FINANCIAL LOSS OR PERSONAL INJURY OR PROPERTY DAMAGE NO AGENT EMPLOYEE OR REPRESENTATIVE OF APPLIED BIOSYSTEMS HAS ANY AUTHORITY TO MODIFY THE TERMS OF THIS LIMITED WARRANTY STATEMENT OR TO BIND APPLIED BIOSYSTEMS TO ANY AFFIRMATION REPRESENTATION OR WARRANTY CONCERNING THE PRODUCT THAT IS NOT Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide B 3 Appendix B Instrument Warranty Information CONTAINED IN THIS LIMITED WARRANTY STATEMENT AN
19. Temperature Verification System be recalibrated once a year For instructions on having your system recalibrated by the manufacturer refer to Appendix A Recalibration 1 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 0 2 mL Probe Assembly 0 2 mL Probe Assembly The 0 2 mL Probe Assembly shown in the figure below consists of a cone that measures the temperature of the sample well The temperature is measured by a thermistor bead in the cone tip GR1914 IMPORTANT This probe assembly must be used only with 0 2 mL wells Digital Thermometer Overview The handheld digital thermometer shown below has a temperature range of 0 to 100 C and is accurate to within 0 1 C GR1915 IMPORTANT Do not try to recalibrate or perform any service on the digital thermometer The only user serviceable component in the unit 1s the battery Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 1 5 Chapter 1 Introduction Precautions When you use the digital thermometer Make sure the probe assembly is connected to the input connector at the top of the digital thermometer To power on the digital thermometer press the On Off switch on its front The temperature measured by the probe assembly appears on the digital thermometer display in degrees Celsius When you complete a te
20. Value Well R3 Date Tested By Probe Serial Thermometer No Serial No 85 C 45 C Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 13 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module Data Sheet Temperature Non Uniformity Test When you run the Temperature Non Uniformity Test record the setpoint values for the wells L1 to L6 and R1 to R6 listed on this data sheet At the end of the Temperature Non Uniformity Test check the values displayed on the 9700 system against the values recorded here Note You can photocopy this page Date Tested By Probe Serial No Thermometer Serial No Setpoint Value 95 C 55 C L1 L2 L3 L4 L5 L6 R1 R2 R3 R4 R5 R6 3 14 Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Temperature Verification Tests for the 4 oystem 9600 This chapter covers OVCIVIOW uela opone E qd d fase eod fede aeE ead abuse coma eee 4 2 Calibration Verification I68E s sexies cep eb Roe oar ie v dod s ULP CRUS 4 3 Temperature Non Uniformity Test 4 8 Data Sheet Calibration Verification Test 4 11 Data Sheet Temperature Non Uniformity Test
21. clock on the run time display will read 0 30 30 seconds Record this temperature The figure below shows when to measure the temperatures Measure well A1 Measure well A4 90 seconds into 90 seconds into Setpoint 1 Setpoint 1 Measure well A1 Measure well A4 90 seconds into Setpoint 2 90 seconds into Setpoint 2 95 Move the Move the probe probe 40 to well A4 to well A8 Temp ns OC Cycle 4 4 After you measure the second temperature of well A1 turn the cover knob completely counterclockwise then slide the heater cover back Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 9 Chapter 4 Temperature Verification Tests for the System 9600 Calculating Test Results Ending the Test 5 Move the probe assembly to the next well to be measured 6 Slide the heater cover forward then turn the cover knob clockwise until the white mark on the knob and the white mark on the cover are aligned 7 Repeat the measurements on wells A4 A8 A12 C1 C4 C8 C12 E1 E4 E8 E12 H1 H4 H8 H12 Note The temperature display on the digital thermometer may not match the temperature display on the system 9600 because the heated sample block cover affects the probe temperature If you suspect any temperature calibration problems perform the Calibration Verification Test described on page 4 3 Use the following information to ca
22. displayed Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 9 Chapter 5 Temperature Verification Tests for the System 2400 TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 94 C Sample must be within 1 0 C of setpoint Cancel F1 F2 F3 F4 F5 Note The sample block must be at 94 C 1 0 C In addition the cover must be at 105 C 1 C It may take several minutes for the system 2400 to ramp up The TNU Performance screen counts down the time until the setpoint is stabilized TNU Performance Sample temp xx x C Cover temp xxx C Stabilizing at setpoint X XKX Cancel FL F2 F3 F4 F5 2 When the Stabilizing at setpoint value decreases to 0 read the digital 4 thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field TNU Performance Sample temp xx x C Cover temp xxx C Enter actual block temperature 00 0 Cancel F1 F2 F3 FA F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the TNU Performance screen Note Record this value on the Temperature Non Uniformity Test Data Sheet page 5 14
23. down IMPORTANT Seat the probe properly and close the heated cover carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Power on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer Configuring the To configure the system 2400 System 2400 Start up the system 2400 The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The Diagnostics screen appears 4 Press F3 TmpVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU gr F1 F2 F3 F4 F5 5 Press F2 TNU The system 2400 is automatically configured for the Temperature Non Uniformity Test and the TNU Performance screen appears TNU Performance Sample temp xx x C Cover temp xxx C Place Probe in well A2 Press Run Run Cancel EJ F2 F3 FA F5 Running the Test The Temperature Non Uniformity Test uses the 0 2 mL Probe Assembly to test the temperature uniformity of 7 different wells in the sample block Note Press F5 Cancel if you want to exit the test To run the Temperature Non Uniformity Test 1 Press F1 Run The Temperature Non Uniformity Test starts and the TNU Performance screen appears with the setpoint value
24. heated cover forward and pull the lever down For the 2700 system pull the lever down to engage the heated cover and 9700 2700 Temperature Verification Frame IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Power on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for a detailed description on operating the Model 4500 digital thermometer Configuring the To configure the system 9700 2700 for the Calibration Verification Test System 9700 2700 1 Power on the system 9700 2700 The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The Diagnostics screen appears 4 Press F3 TmpVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU MESSE F1 F2 F3 FA F5 5 Press F1 Temp The system 9700 2700 is automatically configured for the Calibration Verification Test and the Calibration Verification screen appears Calibration Verification Block temp XX xC Cover tenp soac C Place probe in well A6 Press Run Run Cancel F1 F2 F3 FA F5 Running the Test In this test you take temperature readings of the sample well at two different setpoint temperatures using the
25. may be displayed on Applied Biosystems instruments in combination with the safety symbols described in the preceding section English Francais CAUTION Hazardous chemicals Read the Material Safety Data Sheets MSDSs before handling ATTENTION Produits chimiques dangeureux Lire les fiches techniques de s ret de mat riels avant la manipulation des produits CAUTION Hazardous waste Read the waste profile if any in the site preparation guide for this instrument before handling or disposal ATTENTION D chets dangereux Lire les renseignements sur les d chets avant de les manipuler ou de les liminer CAUTION Hazardous waste Refer to MSDS s and local regulations for handling and disposal ATTENTION D chets dangereux Lire les fiches techniques de s ret de mat riels et la r gulation locale associ es la manipulation et l limination des d chets WARNING Hot lamp AVERTISSEMENT Lampe br lante WARNING Hot Replace lamp with an Applied Biosystems lamp AVERTISSEMENT Composants br lants Hemplacer la lampe par une lampe Applied Biosystems CAUTION Hot surface ATTENTION Surface br lante DANGER High voltage WARNING To reduce the chance of electrical shock do not remove covers that require tool access No user serviceable parts are inside Refer servicing to Applied Biosystems qualified service personnel DANGER Haute tension AVERTISSEMENT Pour vite
26. of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test Calibration Verification Actual temperature at 85 C Actual temperature at 45 C Accept Cane FL F2 F3 F4 F5 6 Ifyou entered values on the Calibration Verification Test Data Sheet page 3 13 compare those values with the actual test results 7 Press F1 Accept Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Calibration Verification Test Evaluating the When the system 9700 completes the Calibration Verification Test one of two Results screens appears See the table below to evaluate the results If the sample block module Then the Is properly calibrated Calibration Verification screen appears with the following message displayed Calibration Verification Calibration is good F1 F2 F3 F4 F5 Does not pass the Calibration Verification Test Calibration Verification screen appears with the following message displayed Calibration Verification Instrument may require service Contact Applied Biosystems Technical Support EE F1 F2 BS FA F5 e Ifthe test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e fthe test fails again contact Applied Biosystems Technical Support Endin
27. separates successive commands you select from a drop down or shortcut menu For example Select File gt Open gt Spot Set Right click the sample row then select View Filter gt View All Runs User Attention Two user attention words appear in Applied Biosystems user documentation Each Words word implies a particular level of observation or action as described below Note Provides information that may be of interest or help but is not critical to the use of the product IMPORTANT Provides information that is necessary for proper instrument operation accurate chemistry kit use or safe use of a chemical Examples of the user attention words appear below Note The size of the column affects the run time Note The Calibrate function is also available in the Control Console IMPORTANT To verify your client connection to the database you need a valid Oracle user ID and password IMPORTANT You must create a separate Sample Entry Spreadsheet for each 96 well microtiter plate Safety Alert Safety alert words also appear in user documentation For more information see Words Safety Alert Words on page viii Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide V Preface Send Us Your Comments Send Us Your Applied Biosystems welcomes your comments and suggestions for improving its Comments user documents You can e mail your comments to techpubs appliedbiosystems c
28. to new customers They also provide MSDSs with the first shipment of a hazardous chemical to a customer after an MSDS has been updated MSDSs provide the safety information you need to store handle transport and dispose of the chemicals safely Each time you receive a new MSDS packaged with a hazardous chemical be sure to replace the appropriate MSDS in your files You can obtain from Applied Biosystems the MSDS for any chemical supplied by Applied Biosystems This service is free and available 24 hours a day To obtain MSDSs 1 Go to https docs appliedbiosystems com msdssearch html 2 In the Search field type in the chemical name part number or other information that appears in the MSDS of interest Select the language of your choice then click Search 3 Find the document of interest right click the document title then select any of the following Open To view the document Print Target To print the document e Save Target As To download a PDF version of the document to a destination that you choose Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Electrical Safety 4 To have a copy of a document sent by fax or e mail select Fax or Email to the left of the document title in the Search Results page then click RETRIEVE DOCUMENTS at the end of the document list 5 After you enter the required information click View Deliver Selected Documents Now
29. value displayed on the digital thermometer in the Enter actual block temperature field TNU Performance Sample temp xx x C Cover temp xxx C Enter actual block temperature 00 0 Cancel F1 F2 F3 FA F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the TNU Performance screen Note Record this value on the Temperature Non Uniformity Test Data Sheet page 3 14 to keep a permanent record of the test 3 10 Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test 4 Press Enter The 9700 system automatically begins the second reading 55 C setpoint and the TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 55 C Sample must be within 1 0 C of setpoint Cne F1 F2 F3 F4 F5 Note The sample block must be at 55 C 1 0 C In addition the cover must be at 105 C 1 0 C It may take several minutes for the 9700 system to stabilize at the setpoint temperature 5 Repeat steps 2 through 4 for the second reading 6 Press Enter The TNU Performance screen appears with the following prompt TNU Performance Sample temp xx x C Cover temp xxx C Place probe in well xx Press Run Run Cancel F1 F2 H3 FA F5 7 For the System 9700 with a manual Dual 96
30. 0 2 mL Probe Assembly Note Press F5 Cancel if you want to end the test To run the Calibration Verification Test 1 Press F1 Run to start the Calibration Verification Test The Calibration Verification screen appears with the setpoint value displayed 2 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Block temp sx x C Cover temp e xc Setpoint is 859 C Cover must be within 10 C of 85 C Cancel F1 F2 Bo F4 F5 Note The cover must be at 85 C 10 C It may take several minutes for the system to ramp up The Calibration Verification screen counts down the time until the setpoint is reached Calibration Verification Block temp xx x C Cover temp xxx C Stabilizing at setpoint X XX Cancel F1 F2 F3 FA BD 2 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer 3 Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field Calibration Verification Block temp xx x C Cover temp xxx c Enter actual block temperature Cancel F1 F2 F3 FA F5 Note The digital thermometer displays a four digit value round this
31. 39 and is shipped with the 0 2 mL Probe Kit P N 4317938 when the 0 2 mL Probe Assembly is purchased separately About the Temperature Verification System 1 2 Description The Temperature Verification System 1s a kit consisting of a probe a digital thermometer frames to prevent ambient air from entering the sample block light mineral oil and cotton swabs The system is used to verify temperatures of the sample block on your thermal cycler The major components of the system are shown in Figure 1 1 on page 1 3 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide About the Temperature Verification System Digital thermometer Frame for system 2400 Frame for system 9700 system 9600 or system 2700 GR1918 Plate for system 9700 with Dual 96 Well Sample Block Module qM v EEY EI C e C Se C C C q LERES oe TES 2 cd ao BLO f e e e e e e e e ac alela 22S 0 gel j e XO O OO O O O O MENS SUE CS qu eye Probe assembly Figure 1 1 Components of the Temperature Verification System Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 1 3 Chapter 1 Introduction Temperature Verification Tests Parts Included Recalibration Use the Temperature Verification System to perform the following tests on your GeneAmp PC
32. 5 Temperature Verification Tests for the System 2400 Calibration Verification Block temp xx x 0 Cover temp xxx C Setpoint is 45 C Cover must be within 30 C of 45 C Cancel EI F2 F3 FA F5 Note The cover must be at 105 C 1 C It may take several minutes for the system to ramp up 5 Repeat steps 2 through 4 for the second reading The system 2400 evaluates the calibration of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test Calibration Verification Actual temperature at 85 C Actual temperature at 45 C Accept Cancel F1 F2 F3 F4 F5 6 If you entered values on the Calibration Verification Test Data Sheet page 5 13 compare those values with the actual test results 7 Press F1 Accept 5 6 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Evaluating the After the system 2400 completes the Calibration Verification Test one of two Results screens appears depending on the test results See the table below to evaluate the results If the sample block module Then the Is properly calibrated Calibration Verification screen appears with the following message displayed Calibration Verification Calibration is qood F1 F2 F3 F4 F5 Does not pass t
33. D ANY SUCH MODIFICATION AFFIRMATION REPRESENTATION OR WARRANTY MADE BY ANY AGENT EMPLOYEE OR REPRESENTATIVE OF APPLIED BIOSYSTEMS WILL NOT BE BINDING ON APPLIED BIOSYSTEMS UNLESS IN A WRITING SIGNED BY AN EXECUTIVE OFFICER OF APPLIED BIOSYSTEMS THIS WARRANTY IS LIMITED TO THE BUYER OF THE PRODUCT FROM APPLIED BIOSYSTEMS AND IS NOT TRANSFERABLE Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties of liability such as liability for gross negligence or wilful misconduct or of remedies or damages as or to the extent set forth above In such countries and jurisdictions the limitation or exclusion of warranties liability remedies or damages set forth above shall apply to the fullest extent permitted by law and shall not apply to the extent prohibited by law Damages Claims and Returns Damages If shipping damage to the product is discovered contact the shipping carrier and request inspection by a local agent Secure a written report of the findings to support any claim Do not return damaged goods to Applied Biosystems without first securing an inspection report and contacting Applied Biosystems Technical Support for a Return Authorization RA number Claims After a damage inspection report is received by Applied Biosystems Applied Biosystems will process the claim unless other instructions are provided Returns Do not return any material without prior notification and authorizati
34. DANGER description viii example viii data sheet system 2400 5 13 5 14 system 2700 2 13 2 14 system 9600 4 11 4 12 system 9700 2 13 2 14 system 9700 with dual 96 well block 3 13 3 14 digital thermometer 1 5 to 1 6 documentation feedback vi E electrical hazard symbol 1x electrical safety xiii electrical shock hazards xiii electrical symbols on instruments ix electromagnetic compatibility standards See EMC standards EMC standards xiv Australian xv Canadian xiv European xv equipment overview 1 2 1 4 European EMC standards xv European safety standards xv G gases compressed xiv general hazard symbol ix guidelines chemical safety xiii H hazard icons accompanying safety alert words viii components vili described viii in documents vili on instruments viii ix Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Index 1 See also hazard symbols See also safety symbols hazard symbols electrical ix general ix hot surface 1x in documents viii laser hazard x moving parts x on instruments 1x See also hazard icons See also safety symbols hazards compressed gases xiv electrical shock xiii moving parts xiv moving lifting instrument xi physical xiv pressurized fluids xiv solvents xiv IMPORTANT description viii example viii installation category xiii instrument operational safety instructions for xi italic text when to use v
35. Equipment for Measurement Control and Laboratory Use Part 1 General Requirements XIV Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Safety and Electromagnetic Compatibility EMC Standards Canadian EMC This instrument has been tested to and complies with ICES 001 Issue 3 Industrial Standard Scientific and Medical Radio Frequency Generators European Safety Safety and EMC This instrument meets European requirements for safety Low Voltage Directive standards 73 23 EEC This instrument has been tested to and complies with standards EN 61010 1 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and EN 61010 2 010 Particular Requirements for Laboratory Equipment for the Heating of Materials EMC The 9700 9600 2700 and 2400 instruments meet European requirements for emission and immunity EMC Directive 89 336 EEC These instruments have been tested to and comply with standard EN 61326 Group 1 Class B for the 9700 2700 and 2400 instruments Group 1 Class A for the 9600 instrument Electrical Equipment for Measurement Control and Laboratory Use EMC Requirements Australian EMC This instrument has been tested to and complies with standard AS NZS 2064 Standards Limits and Methods Measurement of Electromagnetic Disturbance Characteristics C of Industrial Scientific and Medical
36. For Products used only with Risk Group 1 Non Pathogenic Agents reagents buffers and chemicals that have Material Safety Data Sheets Products that have NOT been exposed to any human source mate rial or cell culture including clinical samples 2 This classification of product does not require infectious agent decontamination This product may require chemical decontamination depending on what chemicals were used on this product 3 For Products used with any Risk Group 2 Agents or products that HAVE BEEN exposed to any human source material or cell culture including clinical samples c If your product requires decontamination by an outside service as defined by your User Manual or Applied Biosystems Representative follow all directions for that method of decontamination d Otherwise i Apply a freshly made solution of 1 10 v v dilution of 5 25 sodium hypochlorite e g Clorox bleach with water to applicable product surfaces Follow Universal Precautions and wear all applicable personal protective equipment e g labcoat gloves etc at all times while handling the product ii Place product in a well ventilated area Apply freshly prepared 10 aqueous solution of bleach iii Maintain surfaces in wet condition for 15 minutes A spray bottle may be used to carefully apply this solution iv After 15 minutes wipe dry v Repeatapplication of prepared solution and maintain in wet condition for an additional 15 minutes and again wip
37. Indicates a terminal that can receive or supply alternating current or voltage Indicates a terminal that can receive or supply alternating or direct current or voltage It A OO Safety Symbols The following table describes the safety symbols that may be displayed on Applied Biosystems instruments Each symbol may appear by itself or in combination with text that explains the relevant hazard see Safety Labels on Instruments on page x These safety symbols may also appear next to DANGERS WARNINGS and CAUTIONS that occur in the text of this and other product support documents Symbol Description Indicates that you should consult the manual for further information and to proceed with appropriate caution Indicates the presence of an electrical shock hazard and to proceed with appropriate caution Indicates the presence of a hot surface or other high temperature hazard and to proceed with appropriate caution gt gt gt Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide IX Safety and EMC Compliance Information Symbol Description Indicates the presence of a laser inside the instrument and to proceed with A appropriate caution Indicates the presence of moving parts and to proceed with appropriate caution Safety Labels on Instruments X The following CAUTION WARNING and DANGER statements
38. NETT PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block 5 12 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Data Sheet Calibration Verification Test Data Sheet Calibration Verification Test When you run the Calibration Verification Test record the setpoint values for well B4 on this data sheet At the end of the Calibration Verification Test check the values displayed on the system 2400 against the values recorded here Note You can photocopy this page Setpoint Value Well B4 Date Tested By Probe Serial Thermometer No Serial No 85 C 45 C Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 13 Chapter 5 Temperature Verification Tests for the System 2400 Data Sheet Temperature Non Uniformity Test When you run the Temperature Non Uniformity Test record the setpoint values for the wells listed on this data sheet At the end of the Temperature Non Uniformity Test check the values displayed on the system 2400 against the values recorded here Note You can photocopy this page Date Tested By Probe Serial No Thermometer Serial No Setpoint Value 94 C
39. R Systems Calibration Verification Test Checks the temperature of the sample block against specifications for temperature accuracy Temperature Non Uniformity Test Checks the uniformity of sample block temperature from well to well You can perform these tests as frequently as necessary depending on the type of work your laboratory performs For example a laboratory that does forensic typing may need to validate the instrument before or after running samples to have the sample test results considered valid in a court case Another laboratory may want to validate a protocol Typically run the Calibration Verification Test at least once a year and run the Temperature Non Uniformity Test whenever you suspect variation in well to well temperature Running these tests checks that your thermal cycler is working properly Your kit includes Model 4500 Digital thermometer with 9V battery installed e 0 2 mL Probe Assembly Cotton swabs Light mineral oil Temperature Verification Frames For system 2400 PN N8031076 For system 9600 system 9700 and system 2700 PN 4319613 For system 9700 dual 96 well left and right frames PN 4346188 and PN 4346189 Figure 1 1 on page 1 3 illustrates the major components of the kit Note If any part is damaged or missing contact the shipping carrier and Applied Biosystems immediately See Damages Claims and Returns on page B 4 Applied Biosystems recommends that your
40. Thermal Cycler Temperature Verification System For GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Applie App lec ns AS Copyright 2006 Applied Biosystems All rights reserved Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF NOTICE TO PURCHASER PLEASE REFER TO THE SPECIFIC GENEAMP PCR SYSTEM USER MANUAL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION The Applied Biosystems Thermal Cycler Temperature Verification System is covered by U S Patent No 5 224 778 TRADEMARKS Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the U S and or certain other countries AB Design and Applera are trademarks of Applera Corporation or its subsidiaries in the U S and or certain other countries GeneAmp is a registered trademark of Roche Molecular Systems Inc All other trademarks ar
41. Well Sample Block Module Slide the heated cover forward and pull the lever down For the System 9700 with an Auto Lid Dual 96 Well Sample Block Module Press the red button to slide and close the heated cover Repeat steps 4 through 7 of Setting Up the 0 2 mL Probe Assembly on page 3 8 and steps 2 through 7 of this procedure Complete these steps for all the remaining 11 wells to be tested L2 to L6 and R1 to R6 The 9700 system evaluates the uniformity of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test F1 F2 F3 F4 F5 8 Ifyou entered values on the Temperature Non Uniformity Test Data Sheet page 3 14 compare those values with the actual test results 9 Press F1 Accept Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 11 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module Evaluating the After the 9700 system completes the Temperature Non Uniformity Test the TNU Results Performance screen appears See the table below to evaluate the results If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature TNU Performance TNU at 95 C is xx xx Pass TNU at 55 C is xx xx Pass ances F1 F2 F3 F4 F5 Temperature variation of the sample b
42. al sheet if necessary Has this product been exposed to any radioactive substances If YES which radioactive isotopes Attach separate sheet if necessary If YES describe the procedures used to decontaminate the product of the identified radioactive substances Attach additional sheet if necessary Customer agrees that products will not be shipped until all radioactivity has been removed Ags Has this product been exposed to infectious agents YES L NOLI If YES which infectious agent s Which Biosafety Levels as defined in the 4 Edition Biosafety in Microbiological and Biomedical Laboratories USDHHS CDC NIH Attach separate sheet if necessary If YES describe all the procedures used to decontaminate the product of the identified infectious agents Attach additional sheet necessary The Customer understands and agrees that decontamination is critical to issues of health and safety and that thoroughly completing this Certificate is essential Customer represents and warrants to Applied Biosystems that it performed all decontamination procedures as described in this Certificate and completed this Certificate accurately truthfully and in full Customer hereby assumes all responsibility and liability for and shall defend and indemnify Applied Biosystems against injury or damage of whatever kind incurred by Applied Biosystems its employees contractors and or agents that result directly or indirectly from Customer s breach of t
43. d the heated cover and sample block to avoid being burned by hot components Clean the oil off the sample block Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Temperature Non Uniformity Test Overview Use this test to verify the temperature uniformity of the system 9700 2700 with a 0 2 mL Sample Block Module The Temperature Non Uniformity Test consists of the following subprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 2 8 Configuring the System 9700 2700 2 9 Running the Test 2 10 Evaluating the Results 2 12 Ending the Test 2 12 ANETE PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components Required This test requires the 0 2 mL Sample Block Module Temperature Verification Kit Materials PN 4317939 which includes e Cotton swabs e Light mineral oil e 9700 2700 Temperature Verification Frame e 0 2 mL Probe Assembly e Model 4500 digital thermometer with 9V battery installed Setting Up the To set up the 0 2 mL Probe Assembly 0 2 mL Probe Assembly 1 For system 9700 if the heated cover is in the forward position lift the lever then slide the heated cover back For system 2700 lift the lever and open the hinged h
44. e Block User Guide Temperature Non Uniformity Test 6 Slide the heated cover forward then turn the cover knob clockwise until the white mark on the knob is aligned with the white mark on the cover IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 7 Turn on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer Running the Test To run the Temperature Non Uniformity Test 1 Start up the system 9600 and set up a two temperature CYCL program with the following parameters Setpoint 1 Temperature 95 C Hold Time 2 00 minutes Ramp Time 0 00 minutes Setpoint 2 Temperature 40 C Hold Time 2 00 minutes Ramp Time 0 00 minutes Cycles 99 Note Refer to GeneAmp PCR System 9600 User s Manual P N 0093 8660 for details on how to set up a CYCL program 2 Onthe third cycle measure the temperature of well A1 90 seconds into Setpoint 1 95 C setpoint temperature using the digital thermometer The time remaining clock on the run time display will read 0 30 30 seconds Record this temperature 3 Again on the third cycle measure the temperature of well A1 90 seconds into Setpoint 2 40 C setpoint temperature using the digital thermometer The time remaining
45. e dry vi Asafinal step w ipe down all decontaminated surfaces with clear water to remove residual bleach e Analternative solution may be used if the Customer can provide information indicating that the alternative is as effective as 1 10 dilution household bleach solution Documentation for this shall be provided in written form and attached to the Certificate of Decontamination 4 For Products used with Risk Group 3 or 4 Agents or housed in a Biosafety Level 3 or Biosafety Level 4 Laboratory a Decontamination of Applied Biosystems Products used with Risk Group 3 or 4 or housed in a Biosafety Level 3 or 4 Laboratory must be discussed with and approved by Applied Biosystems Environmental Health and Safety Representative It may not be possible for Applied Biosystems to service or transport these products i Environmental Health and Safety US Western Region Phone 650 638 5088 ii Environmental Health and Safety US Eastern Region Phone 508 383 7835 iii Environmental Health and Safety Europe and Asia Phone 011 44 1793 829146 For the Valve Block 1 Flush the valve block and dry with an appropriate gas Argon Helium or Nitrogen 2 If the valve block is constricted and not able to be flushed properly remove the valve block and label it as a Hazardous Waste Solid and dispose in accordance with all local State Federal Environmental laws and regulations Other Should an alternative decontamination procedure be perfor
46. e the following information to calculate the results of the test Refer to the Results calibration label in your user s manual for the High and Low Offset values Note If there is more than one system 9600 in your laboratory verify that the serial number on the calibration label matches the serial number on the instrument you are testing Average Block Temperature at the 95 C Hold Use the following formula to calculate the average block temperature at the 95 C hold Block Average at 95 C T 95 High Offset If the block average is more than 0 75 C above or below 95 C your system 9600 must be recalibrated For example If the measured temperature of well EI was 95 2 C and the High Offset printed on your calibration label is 0 1 you would make the following calculation Block Average at 95 C 95 2 0 1 95 3 C In this example because 95 3 C does not differ by 0 75 C from your programmed target temperature your instrument would not need to be recalibrated Note The offset is the number of degrees Celsius that the temperature of well EI differed from the average temperature of the block when the instrument was calibrated at the factory Average Block Temperature at the 40 C Hold Use the following formula to calculate the average block temperature at the 40 C hold Block Average at 40 C T 40 Low Offset If the block average is more than 0 75 C above or below 40 C your sy
47. e the sole property of their respective owners Part Number 4319092 Rev D 08 2006 Contents Preface How to Use This Guide eer ns V Send Us Your Comments 3225548448 ab eb oed Cog e E T EOS ee ed E sa dn e D ee aes vi How to Obtain Services and Support Vi oafety and EMC Compliance Information Chapter 1 Chapter 2 Safety Conventions Used in This Document viii oymbols onminstrimellts vds ure ra o toe d Rue 9 agi s ae e eio ad a edi d ge Ix Safety Labels on Instruments cee ee eee eens X General Instrument Safety ri ss 4 a doa bees CR UP AAA o RACE SH PUR POI a ET Xi Chemical Sale uos bowed ese eee dans ERRARE PS Rete eee es eases Xii zzril M P C m xiii Physical Hazard Salely 4 3g ou cette ee E eng x vaudou ipod eed uva XIV Safety and Electromagnetic Compatibility EMC Standards XIV Introduction ADOUE TIS AVIAN scie bcd PR Dopod ee hide Ae a Ap bL uic bat ao e dn 1 2 About the Temperature Verification System 1 2 0 2 mL Probe Assembly 3 52 bog avatares Sos tot ote elm sin ene x tun inerte feces 1 5 Digital TTiSrmotmetelr 542 5 ss ss sien ua RR Sd REN ohne ee he RON E eR Rs 1 5 Temperature Verification Tests for the System 9700 and oystem 2700 S il PsC 2 2 Calibration Verification Test
48. eated cover 2 Place the 9700 2700 Temperature Verification Frame on the sample block 3 Use a cotton swab to coat the following wells with mineral oil A1 A12 C4 C9 F4 F9 H1 H12 4 Place the 0 2 mL Probe Assembly into well A1 Note As the test progresses you move the 0 2 mL Probe Assembly to each of the test wells 5 Thread the probe wire through the channel in the 9700 2700 Temperature Verification Frame to prevent damage to the probe and lead wires See Figure 2 1 on page 2 2 6 Make sure the probe is connected to the digital thermometer 2 8 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test 7 For system 9700 slide the heated cover forward and pull the lever down For system 2700 pull the lever down to engage the heated cover and 9700 2700 Temperature Verification Frame IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Power on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer Configuring the To configure the 9700 2700 system System 9700 2700 Start up the 9700 2700 system The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The
49. ee Leelee yee Calibration Verification Test Temperature Non Uniformity Test Data Sheet Calibration Verification Test Data Sheet Temperature Non Uniformity Test Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module Overview Materials Assembly 3 2 When you perform temperature verification tests on the System 9700 with a Dual 96 Well Sample Block Module you use the materials provided in your kit Figure 3 1 shows the right frame of a properly assembled system Note that the probe wire is threaded through the channel to prevent damage to the wire when the thermal cycler s heated cover is closed 8 CEPS p C C c 2 C oy ECRANS O ei m a cd QM Re LS SI YOOOOOOoO 3 i OEE WOO X o o ENS C RR Figure 3 1 Proper assembly of the Temperature Verification System for System 9700 with a dual 96 well sample block module right frame shown Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Test Overview Use this test to verify the temperature calibration of a system 9700 with a Dual 96 Well Sample Block Module The Calibration Verification Test consists of the following s
50. ents of its Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL sample Block will be free of defects in materials and workmanship for a period of one 1 year from the date the warranty period begins Applied Biosystems will repair or replace at its discretion all defective components during this warranty period After this warranty period repairs and replacement components may be purchased from Applied Biosystems at its published rates Applied Biosystems also provides service agreements for post warranty coverage Applied Biosystems reserves the right to use new repaired or refurbished instruments or components for warranty and post warranty service agreement replacements Repair or replacement of products or components that are under warranty does not extend the original warranty period Applied Biosystems warrants that all optional accessories supplied with its Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL sample Block such as peripherals printers and special monitors will be free of defects in materials and workmanship for a period of ninety 90 days from the date the warranty begins Applied Biosystems will repair or replace at its discretion defective accessories during this warranty period After this warranty period Applied Biosystems will pass on to the buyer to the extent that it is permitted to do so the warranty of the original manufacturer for such access
51. est When you run the Calibration Verification Test record the setpoint values for well A6 on this data sheet At the end of the Calibration Verification Test check the values displayed on the system 9700 2700 against the values recorded here Note You can photocopy this page Date Tested By a Thermometer Serial No Setpoint Value Well A6 85 C 45 C Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 13 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Data Sheet Temperature Non Uniformity Test 2 14 When you run the Temperature Non Uniformity Test record the setpoint values for the wells listed on this data sheet At the end of the Temperature Non Uniformity Test check the values displayed on the system 9700 2700 against the values recorded here Note If desired you can photocopy this page Date Tested By Probe Serial No Thermometer Serial No Setpoint Value 94 C 37 C A1 A12 C4 C9 F4 F9 H1 H12 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Verification Tests for the system 9700 Dual 96 Well Module This chapter covers OVERVIEW cs veo uate eee eaten cule ee etr
52. ety alert words IMPORTANT You must create a separate Sample Entry Spreadsheet for each 96 well microtiter plate AN eue The lamp is extremely hot Do not touch the lamp until it has cooled to room temperature ANITY CHEMICAL HAZARD Formamide Exposure causes eye skin and respiratory tract irritation It is a possible developmental and birth defect hazard Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves EN SNA ELECTRICAL HAZARD Failure to ground the instrument properly can lead to an electrical shock Ground the instrument according to the provided instructions viii Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Symbols on Instruments Symbols on Instruments Electrical The following table describes the electrical symbols that may be displayed on Symbols on Applied Biosystems instruments Instruments Symbol Description Indicates the On position of the main power switch Indicates the Off position of the main power switch Indicates the On Off position of a push push main power switch Indicates a terminal that may be connected to the signal ground reference of another instrument This is not a protected ground terminal Indicates a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument
53. fy that the fuses are properly installed and that the instrument voltage matches the power supply in your laboratory Ethel FIRE HAZARD For continued protection against the risk of ire replace fuses only with fuses of the type and rating specified for the instrument Power PME ELECTRICAL HAZARD Grounding circuit continuity is vital for the safe operation of equipment Never operate equipment with the grounding conductor disconnected MMM ELECTRICAL HAZARD Use properly configured and pproved line cords for the voltage supply in your facility e SN ELECTRICAL HAZARD Plug the system into a properly grounded receptacle with adequate current capacity Overvoltage The Thermal Cycler Temperature Verification System system has an installation Rating overvoltage category of II and is classified as portable equipment Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide xiii Safety and EMC Compliance Information Physical Hazard Safety 2 Moving Parts anne PHYSICAL INJURY HAZARD Moving parts can crush and cut Keep hands clear of moving parts while operating the instrument Disconnect power before servicing the instrument gt MSNM PHYSICAL INJURY HAZARD Do not operate the instrument without the arm shield in place Keep hands out of the deck area when the instrument is spotting Solvents and Nee PHYSICAL INJURY HAZARD Always wear eye pro
54. g the Test To end the test l Press F5 Exit Remove the 0 2 mL Probe Assembly from the sample block Power off the digital thermometer and clean off the oil Wait for the sample block to reach room temperature 725 C then remove the Dual 96 Well Temperature Verification Plates from the sample block ogres PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components Clean the oil off the sample block Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 7 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module Temperature Non Uniformity Test 3 8 Overview Required Materials Setting Up the 0 2 mL Probe Assembly Use this test to verify the temperature uniformity of the system 9700 with a Dual 96 Well Sample Block Module The Temperature Non Uniformity Test consists of the following subprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 3 8 Configuring the System 9700 3 9 Running the Test 3 10 Evaluating the Results 3 12 Ending the Test 3 12 AS erence PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components This test requires the 0 2 mL Sample Block Module Temperature Verification Kit PN 4317939 which i
55. he Calibration Verification Test Calibration Verification screen appears with the following message displayed Calibration Verification Instrument may require service Contact Applied Biosystems Technical Support BEEN F1 F2 F3 FA F5 e Ifthe test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e If the test fails again contact Applied Biosystems Technical Support Ending the Test To end the test 1 Press F5 Exit Remove the 0 2 mL Probe Assembly from the sample block 2 3 Power off the digital thermometer and clean off the oil 4 Wait for the sample block to reach room temperature 725 C then remove the 2400 Temperature Verification Frame from the sample block ANETE PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 7 Chapter 5 Temperature Verification Tests for the System 2400 Temperature Non Uniformity Test Overview Use this test to verify the temperature uniformity of the system 2400 Equipment Required Setting Up the 0 2 mL Probe Assembly The Temperature Non Uniformity Test consists of the following subprocedures which must be done in order
56. he Calibration Verification Test one of two Results screens appears depending on the test results See the table below to evaluate the results If the sample block module Then the Is properly calibrated Calibration Verification screen appears with the following message displayed Calibration Verification Calibration is good 2 6 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test If the sample block module Then the Does not pass the Calibration Verification Test Calibration Verification screen appears with the following message displayed Calibration Verification Instrument may require service Contact Applied Biosystems Technical Support Bo ET F2 F3 FA F5 e Ifthe test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e Ifthe test fails again contact Applied Biosystems Technical Support Ending the Test To l 2 end the test Press F5 Exit Remove the 0 2 mL Probe Assembly from the sample block Power off the digital thermometer and clean off the oil Wait for the sample block to reach room temperature 25 C then remove the 9700 2700 Temperature Verification Frame from the sample block ASN erence PHYSICAL INJURY HAZARD Hot Surface Use care when working aroun
57. he Temperature Non Uniformity Test consists of the following subprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 4 8 Running the Test 4 9 Calculating Test Results 4 10 Ending the Test 4 10 AS ene PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components This test requires the 0 2 mL Temperature Verification Kit PN 4317939 which includes Cotton swabs e Light mineral oil e 9600 Temperature Verification Frame e 0 2 mL Probe Assembly Model 4500 digital thermometer with 9V battery installed To set up the 0 2 mL Probe Assembly 1 Ifthe heated cover is in the forward position turn the knob completely counterclockwise then slide the heated cover back 2 Using a cotton swab coat the following wells with mineral oil Al A4 A8 A12 C1 C4 C8 C12 E1 EA E8 E12 H1 H4 H8 and H12 3 Place the 9600 Temperature Verification Frame on the sample block with the channel facing the front of the instrument 4 Place the 0 2 mL Probe Assembly into well A1 5 Thread the probe wire through the channel in the 9600 Temperature Verification Frame to prevent damage to the probe and lead wires Make sure the probe is connected to the digital thermometer See Figure 4 1 on page 4 2 4 8 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sampl
58. his representation and warranty The Customer accepts that Applied Biosystems has no obligation to repair service or transport any product if this Certificate is not completed in full Company Print Name By Signature Print Name AB Part Number 4343055 Rev B 1 C 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide lt gt Applied E Biosystems Examples of Decontamination Procedures The following are widely used industry standards for decontamination For Chemical buffers reagents carcinogens toxins sensitizers teratogens and mutagens Contaminants 1 Rinse areas associated with chemicals with an acceptable solvent such as methyl alcohol ethyl alcohol or isopropyl alcohol 2 Thenrinse with detergent and water 4 4 For Radioactive Materials 1 Apply an industry standard radioactivity decontaminant e g Radiacwash Rad Con or equivalent to product surfaces and wipe surfaces as directed by decontaminant manufacturer 2 Survey the product with appropriate radioactivity measuring instrument e g Geiger Counter or scintillation counter and attach the results to this form 3 Satisfactory decontamination is defined as survey results at or below local background levels 4 Repeat steps 1 and 2 until achieving satisfactory survey results App For Infectious Agents Customer will evaluate and determine type of decontamination required 1
59. ing the Test To run the Calibration Verification Test 1 Start up the 9600 system The Main menu appears 2 Press the OPTION key three times to move the cursor to UTIL then press ENTER The Utilities menu appears Select Function DIR CONFIG DIAG DEL 3 Press the OPTION key twice to move the cursor to DIAG then press ENTER The following menu appears Enter Diag Test 1 REVIEW History file 4 Press 5 then press ENTER to run the Verify Calibration Diagnostic Test Test 5 Note To ensure maximum accuracy the temperatures of the heated cover and the sample block are the same in this test This prevents the heated cover from affecting the accuracy of the probe assembly The temperature of the sample block and heated cover increases to 40 C and the following display appears Going to 40 C Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within 10 C of the sample block temperature the following display appears Wait 3 minutes Time MM SS Blk 40 0C This display shows the current sample block temperature Blk 40 0C and a clock that counts up from 0 in minutes and seconds Time MM SS 4 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test When the clock reaches 3 minutes the following di
60. ith the actual test results 9 Press F1 Accept Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 11 Chapter 5 Temperature Verification Tests for the System 2400 Evaluating the After the system 2400 completes the Temperature Non Uniformity Test the TNU Results Performance screen appears See the table below to evaluate the results If the Then Temperature of the Pass appears after each setpoint temperature sample block wells is uniform TNU Performance TNU at 94 C is xx xx Pass TNU at 37 C is xx xx Pass Cancel F1 F2 F3 F4 F5 Temperature variation Fail appears after the setpoint temperature s for which the of the sample block test failed wells exceeds performance specifications TNU Performance TNU at 94 C is xx xx Fail TNU at 37 C is xx xx Fail Cancel F1 F2 F3 FA F5 e Ifthe test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e lf the test fails again contact Applied Biosystems Technical Support Ending the Test To end the test 1 Press F5 Cancel 2 Remove the 0 2 mL Probe Assembly from the sample block 3 Power off the digital thermometer and clean off the oil 4 Wait for the sample block to reach room temperature 725 C then remove the 2400 Temperature Verification Frame from the sample block A
61. lace Probe in well L1 Press RUN Cancel Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 9 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module Running the Test The Temperature Non Uniformity Test uses the 0 2 mL Probe Assembly to test the temperature uniformity of 6 different wells in each sample block Note Press F5 Cancel if you want to exit the test To run the Temperature Non Uniformity Test 1 Press F1 Run The Temperature Non Uniformity Test starts and the TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 95 C Sample must be within 1 0 C of setpoint Cancel EL F2 F3 F4 F5 Note The sample block must be at 95 C 1 0 C In addition the cover must be at 105 C 1 0 C It may take several minutes for the system 9700 to stabilize at the setpoint temperature The TNU Performance screen counts down the time until the setpoint is stabilized TNU Performance Sample temp c x C Cover temp xxx c Stabilizing at setpoint Nice Cancel F1 F2 H3 FA F5 2 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer 3 Using the numeric keys type the
62. lculate the results of the test Note If there is more than one system 9600 in your laboratory verify that the serial number on the calibration label matches the serial number on the instrument you are testing For the 16 Setpoint 1 measurements 95 C hold subtract the lowest measured temperature from the highest measured temperature For the 16 Setpoint 2 measurements 40 C hold subtract the lowest measured temperature from the highest measured temperature If either result is greater than 1 C your GeneAmp PCR System 9600 must be serviced by an Applied Biosystems service representative To end the test 1 Remove the 0 2 mL Probe Assembly from the sample block 2 Power off the digital thermometer and clean off the oil 3 Wait for the sample block to reach room temperature 725 C then remove the 9600 Temperature Verification Frame from the sample block AS erence PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 4 Clean the oil off the sample block 4 10 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Data Sheet Calibration Verification Test Data Sheet Calibration Verification Test When you run the Calibration Verification Test record the setpoint values for well E1 on this data sheet At the end of the Calibration Verification Test check the values di
63. lock wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed TNU Performance TNU at 95 C is xx xx Fail TNU at 55 C is xx xx Fail Kaneel F1 F2 F3 FA F5 e lf the test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e lf the test fails again contact Applied Biosystems Technical Support Ending the Test To end the test 1 Press F5 Cancel Remove the 0 2 mL Probe Assembly from the sample block 2 3 Power off the digital thermometer and clean off the oil 4 Wait for the sample block to reach room temperature 25 C then remove the Dual 96 Well Temperature Verification Plates from the sample block AS erence PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block 3 12 Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Data Sheet Calibration Verification Test Data Sheet Calibration Verification Test When you run the Calibration Verification Test record the setpoint values for well R3 on this data sheet At the end of the Calibration Verification Test check the values displayed on the system 9700 against the values recorded here Note You can photocopy this page Setpoint
64. med the Customer shall document the procedure in detail The documentation shall be included with the Certificate of Decontamination as supporting data AB Part Number 4343055 Rev B 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide C 3 Appendix C Certificate of Decontamination C 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Index A Applied Biosystems contacting vi customer feedback on documentation vi Services and Support vi Technical Communications vi Technical Support vi Australian EMC standards xv B bold text when to use v C Calibration Verification Test system 2400 5 3 to 5 7 system 2700 2 3 to 2 7 system 9600 4 3 to 4 7 system 9700 2 3 to 2 7 system 9700 with dual 96 well block 3 3 to 3 7 Canadian safety standards xiv CAUTION description viii example viii chemical safety xii chemical safety guidelines xiii claims processing B 4 compressed fluids hazard xiv compressed gases hazard xiv See also pressurized fluids computer configuration requirement B 2 technical support for altered configuration B 2 conventions bold text v IMPORTANTS v in this guide v italic text v menu commands v Notes v user attention words v conventions safety viii customer feedback on Applied Biosystems documents vi D damage reporting B 4 damaged items returning B 4
65. mpt to lift or move the computer or the monitor without the assistance of others Depending on the weight of the computer and or the monitor moving them may require two or more people Things to consider before lifting the computer and or the monitor Make sure that you have a secure comfortable grip on the computer or the monitor when lifting Make sure that the path from where the object is to where it is being moved is clear of obstructions Do not lift an object and twist your torso at the same time Keep your spine in a good neutral position while lifting with your legs Participants should coordinate lift and move intentions with each other before lifting and carrying nstead of lifting the object from the packing box carefully tilt the box on its side and hold it stationary while someone slides the contents out of the box Ensure that anyone who operates the instrument has Received instructions in both general safety practices for laboratories and specific safety practices for the instrument Read and understood all applicable Material Safety Data Sheets MSDSs See About MSDSs on page xii N ue Before using a cleaning or decontamination method other than those recommended by the manufacturer verify with the manufacturer that the proposed method will not damage the equipment Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Xi Safety a
66. n Temperature of the sample block wells is uniform Pass appears after each setpoint temperature TNU Performance TNU at 94 C is xx xx Pass TNU at 37 C is xx xx Pass Cancel F1 F2 F3 FA F5 Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed TNU Performance TNU at 94 C is xx xx Fail TNU at 37 C is xx xx Fail Cancel F1 F2 F3 FA ius e Ifthe test fails repeat the procedure to make sure the digital thermometer was not misread or that data entry errors were not made e If the test fails again contact Applied Biosystems Technical Support Ending the Test To end the test 1 Press F5 Cancel Remove the 0 2 mL Probe Assembly from the sample block 2 3 Power off the digital thermometer and clean off the oil 4 Wait for the sample block to reach room temperature 25 C then remove the 9700 2700 Temperature Verification Frame from the sample block Are PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 5 Clean the oil off the sample block 2 12 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Data Sheet Calibration Verification Test Data Sheet Calibration Verification T
67. ncludes Cotton swabs e Light mineral oil Left and Right Dual 96 Well Block Temperature Verification Plates e 0 2 mL Probe Assembly Model 4500 digital thermometer with 9V battery installed To set up the 0 2 mL Probe Assembly 1 For the 9700 system with a manual Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then slide the heated cover back For the 9700 system with an Auto Lid Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then press the red button to slide the heated cover back 2 Place both Dual 96 Well Temperature Verification Plates on the sample block The plate labeled with L1 to L6 is for the left sample block The plate labeled with R1 to R6 is for the right sample block Position the plates so the angled corners face away from you Note The plates are used as guides to help you locate the probes within the sample blocks 3 Usea cotton swab to coat wells L1 to L6 and R1 to R6 with mineral oil Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test 4 Place the 0 2 mL Probe Assembly into well L1 As the test progresses you move the 0 2 mL Probe Assembly to each of the test wells L1 to L6 and R1 to R6 5 Thread the probe wire through the channel in the Dual 96 Well Temperature Verification Plate to prevent damage to the probe and lead wires
68. nd EMC Compliance Information Chemical Safety Chemical Hazard Anne CHEMICAL HAZARD Before handling any chemicals refer XII Warning About MSDSs Obtaining MSDSs to the Material Safety Data Sheet MSDS provided by the manufacturer and observe all relevant precautions Pi Attlee CHEMICAL HAZARD All chemicals in the instrument including liquid in the lines are potentially hazardous Always determine what chemicals have been used in the instrument before changing reagents or instrument components Wear appropriate eyewear protective clothing and gloves when working on the instrument rene CHEMICAL HAZARD Four liter reagent and waste bottles can crack and leak Each 4 liter bottle should be secured in a low density polyethylene safety container with the cover fastened and the handles locked in the upright position Wear appropriate eyewear clothing and gloves when handling reagent and waste bottles NUES CHEMICAL STORAGE HAZARD Never collect or store waste in a glass container because of the risk of breaking or shattering Reagent and waste bottles can crack and leak Each waste bottle should be secured in a low density polyethylene safety container with the cover fastened and the handles locked in the upright position Wear appropriate eyewear clothing and gloves when handling reagent and waste bottles Chemical manufacturers supply current Material Safety Data Sheets MSDSs with shipments of hazardous chemicals
69. nd Support Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches 150 countries on six continents For sales office locations and technical support please call our local office or refer to our Web site at www appliedbiosystems com www appliedbiosystems com y m AS Applied D Biosystems Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Printed in USA 08 2006 Part Number 4319092 Rev D an Applera business
70. ng around the heated cover and sample block to avoid being burned by hot components Required This test requires the 0 2 mL Temperature Verification Kit PN 4317939 which Materials includes e Cotton swabs e Light mineral oil e 2400 Temperature Verification Frame e 0 2 mL Probe Assembly e Model 4500 digital thermometer with 9V battery installed Setting Up the To set up the 0 2 mL Probe Assembly 0 2 mL Probe Assembly 1 Ifthe heated cover is in the forward position lift the lever then slide the heated cover back Place the 2400 Temperature Verification Frame on the sample block Using a cotton swab coat well B4 with mineral oil Place the 0 2 mL Probe Assembly into well B4 P d 2m mv Thread the probe wire through the channel in the 2400 Temperature Verification Frame to prevent damage to the probe and lead wires See Figure 5 1 on page 5 2 6 Make sure the probe is connected to the digital thermometer Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 5 3 Chapter 5 Temperature Verification Tests for the System 2400 7 Slide the heated cover forward and pull the lever down IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Power on the digital thermometer Note Refer to the instructions included with your Temperature Verification
71. off to three digits before typing it in the Calibration Verification screen Note Record this value on the Calibration Verification Test Data Sheet page 2 13 to keep a permanent record of the test 4 Press Enter The system 9700 2700 automatically begins the second temperature reading 45 C setpoint The Calibration Verification screen appears with the setpoint value displayed Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 5 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Calibration Verification Block temp XKX x C Cover temp xxx C Setpoint is 45 C Cover must be within 30 C of 45 C Cancel F1 F2 F3 F4 F5 Note The cover must be at 45 C 30 C It may take several minutes for the system to ramp up 5 Repeat steps 2 through 4 for the second temperature reading The system 9700 2700 evaluates the calibration of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test Calibration Verification Actual temperature at 85 C Actual temperature at 45 C Accept Cancel FL F2 F3 F4 F5 6 Ifyou entered values on the Calibration Verification Test Data Sheet page 2 13 compare those values with the actual test results 7 Press F1 Accept Evaluating the When the system 9700 2700 completes t
72. om How to Obtain Services and Support For the latest services and support information for all locations go to http www appliedbiosystems com then click the link for Services and Support At the Services and Support page you can e Search through frequently asked questions FAQs e Submit a question directly to Technical Support Order Applied Biosystems user documents MSDSs certificates of analysis and other related documents Download PDF documents Obtain information about customer training Download software updates and patches In addition the Services and Support page provides access to worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities vi Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide oafety and EMC Compliance Information This section includes the following topics Safety Conventions Used in This Document vili SVMDOlS ON I str tie ness d ope ET Me be ee aa SOL Rd aiat Boh bo n 1X Safety Labels on Instruments X GeneralInstr ment Sa3feby ris nr pe pe HUE eti ce at puce xi Chemical Saey sacret ne rai e EO a E Lee es xii Electrical Sale ccc ect ntaa eara P HL ILES UD xiii Physical Hazard SE anse irae ohn cae E E s mter Edo me deed XIV Safety and Electromagnetic Compatibility EMC Standards XIV Thermal
73. on If for any reason it becomes necessary to return material to Applied Biosystems contact Applied Biosystems Technical Support or your nearest Applied Biosystems subsidiary or distributor for a return authorization RA number and forwarding address Place the RA number in a prominent location on the outside of the shipping container and return the material to the address designated by the Applied Biosystems representative B 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Certificate of Decontamination The following form is supplied to you for the purpose of shipping your instrument to Applied Biosystems for repair or service and or prior to any on site service to be performed by Applied Biosystems A completed copy of the Certificate of Decontamination must be attached to the instrument or part itself and a copy of this certificate must be attached to the outside of the packing container when transporting to an Applied Biosystems facility Print out and enter the requested information as appropriate Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide C 1 Appendix C Certificate of Decontamination lt gt Applied ES Biosystems CERTIFICATE OF DECONTAMINATION This document must be completed in full and signed by the Customer a before transporting by any means a product to Applied Biosystems for repair o
74. ories With the exception of consumable and maintenance items replaceable products or components used on or in the instrument are themselves warranted to be free of defects in materials and workmanship for a period of ninety 90 days Applied Biosystems warrants that chemicals and other consumable products will be free of defects in materials and workmanship when received by the buyer but not thereafter unless otherwise specified in documentation accompanying the product Applied Biosystems warrants that for a period of ninety 90 days from the date the warranty period begins the tapes diskettes or other media bearing the operating software of the product if any will be free of defects in materials and workmanship under normal use If there 1s a defect in the media covered by the above warranty and the media is returned to Applied Biosystems within the ninety 90 day warranty period Applied Biosystems will replace the defective media Applied Biosystems does not warrant that the operation of the instrument or its operating software will be uninterrupted or error free B 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Limited Product Warranty Warranty Period Any applicable warranty period under these sections begins on the earlier of the date Effective Date of installation or ninety 90 days from the date of shipment for hardware and software installed by Applied Biosys
75. perature Non Uniformity Test 2 8 Data Sheet Calibration Verification Test 2 13 Data Sheet Temperature Non Uniformity Test 2 14 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 1 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Overview Materials When you perform temperature verification tests on the System 9700 with a 96 Well sample Block Module or System 2700 you use the materials provided in your kit Assembly Figure 2 1 shows a properly assembled system Note that the frame is positioned around the wells with the channel facing you and the probe wire is threaded through the channel to prevent damaging the wire when the thermal cycler s heated cover is closed Figure 2 1 Proper assembly of the Temperature Verification System for System 9700 and System 2700 2 2 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Calibration Verification Test Overview Required Materials Setting Up the 0 2 mL Probe Assembly Use this test to verify the temperature calibration of your System 9700 2700 with a 0 2 mL Sample Block Module The Calibration Verification Test consists of the following subprocedures which must be done in order Subprocedu
76. ple temp xx x C Cover temp xxx C Stabilizing at setpoint X XX Cancel F1 F2 F3 F4 F5 2 When the Stabilizing at setpoint value decreases to 0 read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer 3 Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field TNU Performance Sample temp xx x C Cover temp xxx C Enter actual block temperature 00 0 Cancel ET F2 F3 F4 F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the TNU Performance screen Note Record this value on the Temperature Non Uniformity Test Data Sheet page 2 14 to keep a permanent record of the test 2 10 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Non Uniformity Test 4 Press Enter The system 9700 2700 automatically begins the second reading 94 C setpoint and the TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 94 C Sample must be within 1 0 C of setpoint Cancel F1 F2 F3 FA ES Note The sample block must be at 94 C 1 0 C In addition the cover must be at 105 C 1 C I
77. r GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide A 3 Appendix A Recalibration A 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Instrument Warranty Information This appendix contains the following Computer Conto UtAHOD s s ooa da tese peS i b eREPS UI hous adhere eT ETE B 2 Limited Product Warranty un acean urbe dob be PRESE E MEE us B 2 Damages Claims and Returns B 4 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide B 1 Appendix B Instrument Warranty Information Computer Configuration Applied Biosystems supplies or recommends certain configurations of computer hardware software and peripherals for use with its instrumentation Applied Biosystems reserves the right to decline support for or impose extra charges for supporting nonstandard computer configurations or components that have not been supplied or recommended by Applied Biosystems Applied Biosystems also reserves the right to require that computer hardware and software be restored to the standard configuration prior to providing service or technical support For systems that have built in computers or processing units installing unauthorized hardware or software may void the Warranty or Service Plan Limited Product Warranty Limited Warranty Applied Biosystems warrants that all standard compon
78. r les risques d lectrocution ne pas retirer les capots dont l ouverture n cessite l utilisation d outils L instrument ne contient aucune piece r parable par l utilisateur Toute intervention doit tre effectu e par le personnel de service qualifi de Applied Biosystems CAUTION Moving parts ATTENTION Parties mobiles Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide General Instrument Safety General Instrument Safety Moving and Lifting the Instrument Moving and Lifting Stand Alone Computers and Monitors Operating the Instrument Cleaning or Decontaminating the Instrument Nee PHYSICAL INJURY HAZARD Use this product only as specified in this document Using this instrument in a manner not specified by Applied Biosystems may result in personal injury or damage to the instrument N en Yale PHYSICAL INJURY HAZARD The instrument is to be moved and positioned only by the personnel or vendor specified in the applicable site preparation guide If you decide to lift or move the instrument after it has been installed do not attempt to lift or move the instrument without the assistance of others the use of appropriate moving equipment and proper lifting techniques Improper lifting can cause painful and permanent back injury Depending on the weight moving or lifting an instrument may require two or more persons Nem Do not atte
79. r service and b prior to any on site service to be performed by Applied Biosystems A completed copy of this Certificate is required to be attached to the instrument part itself AND to the outside of the packing container when transporting to an Applied Biosystems Facility SUPPLY THE FOLLOWING INFORMATION ABOUT THE APPLIED BIOSYSTEMS PRODUCT Examples of Decontamination Procedures are described on the back of this sheet Instrument Model Type OR Part Name Instrument or Part Serial Number Reason for return service RMA or RA number required if product is to be returned to Applied Biosystems Field Service Engineer Name if applicable Has this product been used YESL NOL If YES the next question should be checked YES with all supporting information provided If NO then this product was received non functioning andlor NO buffers reagents chemicals or samples of any nature were used with or on this product Has this product been exposed to any buffers reagents andlor chemical s YES L NO Chemicals include but are not limited to carcinogens sensitizers mutagens teratogens and toxics Determine classification of chemical from chemical label or MSDS If YES list all buffers reagents and or chemicals used on or with this product Attach additional sheet if necessary If YES describe the procedures used to decontaminate the product of the identified buffers reagents and or chemicals Attach addition
80. re See Page Setting Up the 0 2 mL Probe Assembly 2 3 Configuring the System 9700 2700 2 4 Running the Test 2 4 Evaluating the Results 2 6 Ending the Test 2 7 AS ene PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components This test requires the 0 2 mL Sample Block Module Temperature Verification Kit PN 4317939 which includes Cotton swabs Light mineral oil 9700 2700 Temperature Verification Frame 0 2 mL Probe Assembly Model 4500 Digital thermometer with 9V battery installed To set up the 0 2 mL Probe Assembly l ue dm IO Mos For system 9700 if the heated cover is in the forward position lift the lever then slide the heated cover back For system 2700 lift the lever and open the hinged heated cover Place the 9700 2700 Temperature Verification Frame on the sample block Using a cotton swab coat well A6 with mineral oil Place the 0 2 mL Probe Assembly into well A6 Thread the probe wire through the channel in the 9700 2700 Temperature Verification Frame to prevent damage to the probe and lead wires See Figure 2 1 on page 2 2 Make sure the probe is connected to the digital thermometer Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 3 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 7 For the 9700 system slide the
81. splay appears Record Temperature Time MM SS Blkz95 0C 5 Measure the temperature of well E1 using the digital thermometer Record this temperature as T 40 6 Press ENTER The temperature of the sample block and heated cover goes to 95 C and the following display appears Going to 9596 Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within 10 C of the sample block temperature the following display appears Wait 3 Minutes Time MM SS Blk 95 0C This display shows the current sample block temperature BIk 95 0C and a clock that counts up from 0 in minutes and seconds Time MM SS When the clock reaches 3 minutes the following display appears Record Temperature Time MM SS Blk 95 0C 7 Measure the temperature of well E1 using the digital thermometer Record this temperature as T 95 IMPORTANT To end the test at any time press the STOP key This returns you to the Review History File display Press 5 and then press ENTER to return to the Verify Calibration Diagnostic test The figure below illustrates temperatures during the Calibration Verification Test Time in minutes Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 5 Chapter 4 Temperature Verification Tests for the System 9600 Calculating Test Us
82. splayed on the system 9600 against the values recorded here Note You can photocopy this page Setpoint Value Well E1 Date Tested By Probe Serial Thermometer No Serial No 95 C 40 C Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 11 Chapter 4 Temperature Verification Tests for the System 9600 Data Sheet Temperature Non Uniformity Test 4 12 When you run the Temperature Non Uniformity Test record the setpoint values for the wells listed on this data sheet At the end of the Temperature Non Uniformity Test check the values displayed on the system 9600 against the values recorded here Note You can photocopy this page Date Tested By Probe Serial No Thermometer Serial No Setpoint Value 95 C 40 C A1 A4 A8 A12 C1 C4 C8 C12 E1 E4 E8 E12 H1 H4 H8 H12 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Verification Tests for the oystem 2400 This chapter covers OVCPVIC Wt ua opea E qd dando esa neds qua iecdid Gs rd Rad erp 5 2 Calibration Verification Te8E s se sire rcp eb Roe ear dev dora ser cad leurs 5 3 Temperature Non Uniformity Test
83. st move the on off switch to power off the digital thermometer Temperature The sample temperature displayed on the thermal cycler is different from the Display temperature displayed on the digital thermometer during heating or cooling Differences transitions This is because the thermal cycler measures sample temperature but the digital thermometer measures block temperature The sample temperature displayed by the thermal cycler is a function of the tube type and the reaction volume Using the Probe Applied Biosystems recommends that you use the probe assembly and digital While Running thermometer only for the temperature Calibration Verification Test and the Programs Temperature Non Uniformity Test which are described in this manual If you use the probe assembly and digital thermometer while running programs other than those used in these two tests be aware that the accuracy of the probe assembly decreases due to the effect of the heated sample block cover The heated cover normally operates at 105 C The effect of the heated cover on the probe assembly decreases as the temperature of the sample block approaches 99 9 C 1 6 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Temperature Verification Tests for the P System 9700 and System 2700 This chapter covers OVCIVIOW ET E EEE E T EE E EA ENE A EN E E E EE 2 2 Calibration Verification Tests nina min mamie 2 3 Tem
84. stem 9600 must be recalibrated For example If the measured temperature of well EI was 39 9 C and the Low Offset printed on your calibration label is 0 1 you would make the following calculation Block Average at 40 C 39 9 40 1 39 8 C In this example because 39 8 C does not differ by 0 75 C from your programmed target temperature your instrument would not need to be recalibrated 4 6 Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide Calibration Verification Test Ending the Test When you have completed all measurements end the test To end the test 1 Remove the 0 2 mL Probe Assembly from the sample block 2 Power off the digital thermometer and clean off the oil 3 Wait for the sample block to reach room temperature 725 C then remove the 9600 Temperature Verification Frame from the sample block ASN erence PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components 4 Clean the oil off the sample block Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 7 Chapter 4 Temperature Verification Tests for the System 9600 Temperature Non Uniformity Test Overview Use this test to verify the temperature uniformity of the system 9600 Required Materials Setting Up the 0 2 mL Probe Assembly T
85. t may take several minutes for the system 9700 2700 to stabilize at the setpoint temperature 5 Repeat steps 2 through through 4 for the second reading 6 Press Enter The TNU Performance screen appears with the following prompt TNU Performance Sample temp xx x C Cover temp exc Place probe in well xx Press Run Run Cancel F1 F2 F3 F4 F5 7 Slide the heated cover back system 9700 or open the hinged heated cover system 2700 Repeat steps 4 through 7 of Setting Up the 0 2 mL Probe Assembly on page 2 8 and steps 2 through 7 of this procedure Complete these steps for all 8 wells to be tested A1 A12 C4 C9 F4 F9 H1 H12 8 The system 9700 2700 evaluates the uniformity of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test F1 F2 F3 FA F5 If you entered values on the Temperature Non Uniformity Test Data Sheet page 2 14 compare those values with the actual test results 9 Press F1 Accept Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 2 11 Chapter 2 Temperature Verification Tests for the System 9700 and System 2700 Evaluating the When the system 9700 2700 completes the Temperature Non Uniformity Test the Results TNU Performance screen appears See the table below to evaluate the results If the The
86. te the probes within the sample blocks Using a cotton swab coat well R3 with mineral oil Place the 0 2 mL Probe Assembly into well R3 Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 3 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module 5 Thread the probe wire through the channel in the Dual 96 Well Temperature Verification Plate to prevent damage to the probe and lead wires See Figure 3 l on page 3 2 6 Make sure the probe is connected to the digital thermometer 7 For System 9700 with manual Dual 96 Well Sample Block Module Slide the heated cover forward then pull the lever down For System 9700 with Auto Lid Dual 96 Well Sample Block Module Press the red button to slide and close the heated cover IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Power on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer Configuring the To configure the 9700 system for the Calibration Verification Test System 9700 1 Start up the 9700 system The Main menu appears 2 Press F4 Util The Utilities screen appears 3 Press F1 Diag The Diagnostics screen appears 4 Press F3 TmpVer The Temperature Verifica
87. tection Pressurized when working with solvents or any pressurized fluids Fluids Nei PHYSICAL INJURY HAZARD To avoid hazards associated with high pressure fluids in polymeric tubing e Be aware that PEEK tubing is a polymeric material Use caution when working with any polymer tubing that is under pressure Always wear eye protection when in proximity to pressurized polymer tubing Extinguish all nearby flames if you use flammable solvents Do not use PEEK tubing that has been severely stressed or kinked Donotuse PEEK tubing with tetrahydrofuran or concentrated nitric and sulfuric acids Beaware that methylene chloride and dimethyl sulfoxide cause PEEK tubing to swell and greatly reduce the rupture pressure of the tubing e Be aware that high solvent flow rates 740 mL min may cause a static charge to build up on the surface of the tubing Electrical sparks may result Safety and Electromagnetic Compatibility EMC Standards This section provides information on U S and Canadian Safety Standards Canadian EMC Standard European Safety and EMC Standards Australian EMC Standards U S and This instrument has been tested to and complies with standard UL 3101 1 Safety Canadian Safety Requirements for Electrical Equipment for Laboratory Use Part 1 General Standards Requirements U This instrument has been tested to and complies with standard CSA 1010 1 Safety c YL Jus Requirements for Electrical
88. tems personnel For all hardware and software installed by the buyer or anyone other than Applied Biosystems and for all other products the applicable warranty period begins the date the product is delivered to the buyer Warranty Claims Warranty claims must be made within the applicable warranty period or for chemicals or other consumable products within thirty 30 days after receipt by the buyer Warranty The above warranties do not apply to defects resulting from misuse neglect or Exceptions accident including without limitation operation with incompatible solvents or samples in the system operation outside of the environmental or use specifications or not in conformance with the instructions for the instrument system software or accessories improper or inadequate maintenance by the user installation of software or interfacing or use in combination with software or products not supplied or authorized by Applied Biosystems and modification or repair of the product not authorized by Applied Biosystems THE FOREGOING PROVISIONS SET FORTH APPLIED BIOSYSTEMS SOLE AND EXCLUSIVE REPRESENTATIONS WARRANTIES AND OBLIGATIONS WITH RESPECT TO ITS PRODUCTS AND APPLIED BIOSYSTEMS MAKES NO OTHER WARRANTY OF ANY KIND WHATSOEVER EXPRESSED OR IMPLIED INCLUDING WITHOUT LIMITATION WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF DEALING OR USAGE OF
89. tion Kit PN 4317939 which includes Cotton swabs Light mineral oil 9600 Temperature Verification Frame 0 2 mL Probe Assembly Model 4500 digital thermometer with 9V battery installed To set up the 0 2 mL Probe Assembly l i ee iw 4 If the heated cover is in the forward position turn the knob completely counter clockwise then slide the heated cover back Place the 9600 Temperature Verification Frame on the sample block Using a cotton swab coat well E1 with mineral oil Place the 0 2 mL Probe Assembly into well E1 Thread the probe wire through the channel in the 9600 temperature verification frame to prevent damage to the probe and lead wires See Figure 4 1 on page 4 2 Make sure the probe is connected to the digital thermometer Thermal Cycler Temperature Verification System for GeneAmp PCR Systems with a 0 2 mL Sample Block User Guide 4 3 Chapter 4 Temperature Verification Tests for the System 9600 7 Slide the heated cover forward then turn the cover knob clockwise until the white mark on the knob is aligned with the white mark on the cover IMPORTANT The probe must be seated properly and the heated cover closed carefully If the probe wire is crushed when the heated cover is closed the probe can be damaged 8 Turn on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for a detailed description on operating the Model 4500 digital thermometer Runn
90. tion screen appears Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU Exit F1 F2 F3 FA F5 5 Press F1 Temp The 9700 system is automatically configured for the Calibration Verification Test and the Calibration Verification screen appears Calibration Verification Block temp xx x C Cover temp KXXYC Place probe in well R3 Press Run Run Cancel 3 4 Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide Calibration Verification Test Running the Test In this test you take temperature readings of the sample well at two different set points using the 0 2 mL Probe Assembly Note Press F5 Cancel if you want to exit the test To run the Calibration Verification Test 1 Press F1 Run The Calibration Verification screen appears with the setpoint value displayed Calibration Verification Block temp xx x C Cover temp xxx 9C Setpoint is 85 C Cover must be within 1 C of 105 C Cancel F1 F2 F3 FA F5 Note The cover must be at 105 C 1 C It may take several minutes for the system to ramp up The Calibration Verification screen counts down the time until the setpoint is reached Calibration Verification Block temp xx x C Cover temp xxx C Stabilizing at setpoint XxX Cancel F1 F2 F3 F4 F5 2 When the Stabilizing at setpoint
91. ubprocedures which must be done in order Subprocedure See Page Setting Up the 0 2 mL Probe Assembly 3 3 Configuring the System 9700 3 4 Running the Test 3 0 Evaluating the Results 3 7 Ending the Test 3 7 ANETE PHYSICAL INJURY HAZARD Hot Surface Use care when working around the heated cover and sample block to avoid being burned by hot components Required This test requires the 0 2 mL Sample Block Module Temperature Verification Kit Materials PN 4317939 which includes Cotton swabs Light mineral oil Left and Right Dual 96 Well Block Temperature Verification Plates 0 2 mL Probe Assembly Model 4500 digital thermometer with 9V battery installed Setting Up the To set up the 0 2 mL Probe Assembly 0 2 mL Probe Assembly 4 l For System 9700 with a manual Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then slide the heated cover back For System 9700 with an Auto Lid Dual 96 Well Sample Block Module If the heated cover is in the forward position lift the lever then press the red button to slide the heated cover back Place both Dual 96 Well Temperature Verification Plates on the sample block The plate labeled with L1 to L6 is for the left sample block The plate labeled with R1 to R6 is for the right sample block Position the plates so the angled corners face away from you Note The plates are used as guides to help you loca
92. value decreases to 0 read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for details on operating the Model 4500 digital thermometer 3 Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field Calibration Verification Block temp xx x C Cover temp Xxx C Enter actual block temperature Cancel F1 F2 F3 F4 F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the Calibration Verification screen Note Record this value on the Calibration Verification Test Data Sheet page 3 13 to keep a permanent record of the test Thermal Cycler Temperature Verification System for a 0 2 mL Sample Block User Guide 3 5 Chapter 3 Temperature Verification Tests for the System 9700 Dual 96 Well Module 3 6 4 Press Enter The 9700 system automatically begins the second reading 45 C setpoint and the Calibration Verification screen appears with the setpoint value displayed Calibration Verification Block temp s06 xX 0C Cover temp xxcC Setpoint is 45 C Cover must be within 1 C of 105 C Cancel E F2 F3 FA F5 Note The cover must be at 105 C 1 C It may take several minutes for the system to ramp up Repeat steps 2 through 4 for the second temperature reading The 9700 system evaluates the calibration

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