ImageMaster VDS - GE Healthcare Life Sciences
Contents
1. Video System N umber of pixels System type Scanning system M inimum illumination Electronic shutter Gain control Resolution Signal to noise ratio Gamma correction Frame store Video output Power supply Safety Power consumption 110 120 V 60 Hz US model fuses 2 F 4A 250V 220 240 V 50 Hz European model fuses 2 F 2 5A 250V 100 V 50 60 Hz Japanese model fuses 2 F 4A 250V 300 VA M ax 10 40 C Relative Humidity 0 90 noncondensing Indoor use pollution degree 2 O vervoltage category Il Non Operating Environment 10 60 C relative humidity 95 max Lamps shut off after 10 minutes of inactivity O pen and close either door to reactivate Composite video signal 1 0 Vpp at 75 ohm BNC connector 25 pin D type male parallel communication port 645 mm H x 528 mm W x 3 90 mm D 795 h x 596 v US and J apanese models 811 h x 508 v European model NTSC US and J apanese models PAL European model 2 1 Interlace 0 2 Lux at f 1 2 amp shutter speed 1 50 0 0004 Lux at f 1 2 with 500 fields integration 1 100 sec PAL or 1 120 sec NTSC up to 10 sec Auto black restoration or manual selectable M ore than 570 horizontal TV lines M ore than 46db with AGC off 0 45 or 1 0 selectable STILL function button with field frame selection Composite video signal 1 0 V pp at 75 ohm 85 to 264 V at 47H z to 440H z automatic IEC 601 UL544 approved 12 W 8 Spec2. 5 O perating Instructions The PRESETS keys Five sets of parameters can be recalled by pressing the appropriate PRESETS key PRESET 1 is programmed at the factory for optimal UV fluorescence imaging PRESET 2 for white light transillumination and PRESET 5 for white light reflectance All PRESETS keys can be programmed using the SET menu option on the monitor see section 3 4 The SELECT keys The arrow SELECT keys move the cursor among the menu options on the monitor and the and SELECT keys increase or decrease the displayed value T U O O O 0 0 i DIAO IE T T moves the cursor to the left ites a 5 5 moves the cursor to the right ImageMaster VDS increases the value displayed decreases the value displayed Figure 5 Camera control panel TheDATA key The DATA function is used only if the optional LISCAP or ImageM aster VDS software and the Image Capture Board are installed on a PC computer and connected to the ImageM aster V DS system Pressing DATA sends the digitized image to the external computer While the DATA indicator lamp is lit the monitor may appear blank or noisy during the data transfer Under Windows 3 1 Windows 95 or 98 the data transfer take one second Under Windows NT the data transfer can take up to 20 seconds The STILL key The STILL function captures the most recent image in the camera memory and dis
3. controls 9 3 3 Video camera electronic controls 10 3 4 M enu options displayed on the monitor aml Ho PriNt samire eri pier ewes tweed orate AEE ii ee ena 12 4 Quick reference Guidelines a 14 5 Operating instructions 16 5 1 Preliminary Steps Li 16 5 2 UV transillumination procedure cc eee eee ee 17 5 3 White light imaging procedure ccc eee 19 6 Troubleshooting 00 cece eee eee es 21 7 Care and maintenance 0 ee 23 UL CACAMNG s sint i ta 23 7 2 Replacing lamps andina eee 23 7 3 Replacing fuses eran aa 25 74 Handling WM sos renne axe ea a Sheek caves 26 7 5 Cleaning the Printer pineta ein a bah avis 29 7 0 Printer presets i ce ila des iena vas hi 32 8 Specifications 34 9 Customer service information 36 9 1 Technical service and repair ee 36 9 2 Ordering informatin 352044 ia 36 1 Gel Documentation System Function and Description l Packing list M ain cabinet with monitor and camera body UV sample tray Zoom lens assembly zoom close up 2X diopter and orange filter Power cord Super VDS film 1 roll plus two shafted flanges User manuals 3 ImageM aster VDS monitor Sony and thermal imager Fuji Gel Documentation System Function and Description The ImageM aster VDS video documentation system is designed to quickly and accurately document electrophoresis results The basic system which include
4. until the image is properly sized and sharply focused Note If the UV tray isin place and the TRANS mode is selected pressing BY PASS turns on the UV lamps This is not recommended for focusing Use the REFLECT mode without UV light A ruler beside the gel is a convenient focusing target Close the compartment door once these adjustments are satisfactory and select TRANS Fine tune the electronic camera controls using the camera control panel The recommended starting point settings are B lev 6 Gain 0 Gamma 0 45 for video and printer images or 1 0 for transfer to a computer Unless the settings have been reprogrammed PRESET 1 is factory set for optimal UV imaging First optimize aperture and time settings and if further image adjustment is required try incrementally increasing the gain default 0 or decreasing the B lev default 6 N ote Changing the B Lev setting may reduce the dynamic range for instance decreasing the value removes part of the signal This could be useful in removing background noise O nce the image is optimized press the STILL key to hold and display on the monitor the image currently stored in camera memory Note Pressing the STILL key stores the image so that the sample can be removed from the UV tray to minimize photoknicking Pressing the STILL key stores the information but is not necessary to send the image to the printer or computer Print the image by pressing the PRINT key on
5. using for example the ImageM aster Software or for editing annotating or output to a printing device used for publication Unpacking Unwrap all packages carefully and compare contents with the list on this page making sure all items arrived If any part is missing contact your local Amersham Biosciences sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or for repacking should it become necessary to return the unit Note Retain the sheet documenting the factory set optimized parameters for your system for future reference 1 Gel Documentation System Function and Description Important system information Under normal operation of the ImageM aster V DS system the researcher is not exposed to UV light All UV lamps automatically turn off when any door is opened or when the sample tray is pulled out Pressing the BY PASS switch on the lamp control panel overrides the safety feature for the top compartment door however Prolonged exposure to UV light is harmful If you choose to operate the UV lamps while the top compartment is open be sure to wear protective clothing and UV blocking glasses to shield yourself The rear panels should only be removed by trained service technicians due to the danger of exposure to high voltage The AC power outlet must
6. 14 Cleaning the roller 2 Turn the printer power OFF and then ON to reset the printer mode TheLED will indicate error condition 1 and an audible beep will sound 3 M oisten a cotton swab with water to clean the roller do not use gauze or cloth because they may catch in the roller 29 7 Care and Maintenance 30 4 Press the REPRINT key to start the rubber roller rotating It will rotate for about 30 seconds The LED will first indicate error 1 and then cleaning mode CL1 Clean the roller with the cotton swab as it rotates Once the roller stops the printer will again display the error 1 message If the roller is not sufficiently clean repeat step 4 5 Allow the roller to dry 6 M ove the thermal head release lever into the RELEASE position 7 Reload the film as described in section 7 4 l Loading a roll of film ss as steps 5 to 7 Thermal head It is good practice to run the cleaning sheet after every roll of film to prevent printing quality problems such as blotches and streaks The maximum Figure 15 Cleaning the thermal head recommended interval between cleaning sheet runs is three rolls of film approximately 500 prints U se only the accessory cleaning sheet to clean the thermal head 1 Remove the film according to the instruction in section 7 4 Removing a roll of film 2 Lift the roll of film out Turn the printer power OFF and then ON to reset the printer mode The LED will ind
7. ImageMaster VDS Video Documentation System for Gel Electrophoresis User Manual 80 6254 80 Ame rsh am Edition AB e KY Biosciences Important user information Please read these instructions carefully before using and of the above prepacked columns or bulk media Should you have any comments on this instruction manual we will be pleased to receive them at Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences SE 751 84 Uppsala Sweden Amersham BiosciencesInc 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 USA Terms and Conditions of Sale All goods and services are subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Trademarks ImageM aster M ultiphor and PhasteGel are trademarks of Amersham Biosciences Limited or its subsidiaries AmershamBiosciences is a trademark of Amersham plc Fuji is a trademark of Fuji Inc Windows is a trademark of M icrosoft Corp Amersham Biosciences AB 1998 All rights reserved Contents Contents 1 Gel Documentation System Function and Description 3 2 Assembly and Installation 6 3 System Component Close up 0 0 00 ccc eee ees 8 3 1 Lamp controls 2 202 oot done eS eee es beer as Sadi api 8 3 2 Video camera manual
8. Initialization is complete when BOTH Please wait for initialization disappears from the monitor and the Lamps Control indicator signal OK remains lit stops flashing S To initialize install the lens cap SN31 WOOZ gt recommended or set the aperture to f 22 Close all the doors Then turn the mains power switch on Figure 7 Initializing the camera UV transillumination procedure 1 Follow the preliminary steps in the previous section M ake sure you have chosen the UV sample tray that the sample is in place and that the tray is properly installed Verify that the video camera is initialized and the orange filter is installed on the camera lens for ethidium bromide stained gels Set the camera aperture to f 1 2 maximum opening Switch the main lamp control switch on the lamp control panel to ON and choose REFLECT for initial image focusing The top compartment door must remain open while you adjust the camera zoom and focus To switch on the light while the door is open you 17 5 Operating Instructions N ote Adjust only one parameter at a time and only by small increments Note If no activity is detected for 10 minutes the lamps automatically shut off To reactivate open and close either door The image may be captured by pressing STILL or DATA before the lamps switch off 18 must press the BY PASS switch on the lamp control panel continuously Adjust the zoom and focus
9. White light 8 W 1 80 6249 29 Tube UV light 8 W 312 nm 1 80 6249 48 Lens filters Lens Filter orange 1 80 6248 53 Lens Filter blue 1 80 6248 91 Lens Filter yellow 1 80 6249 10 Film VDS Film 4 Rolls 14m 1 80 6249 67 Super VDS Film 4 Rolls 14m 1 80 6249 86 Film holders shafted flanges 2 80 6352 65 Printer cleaning sheet 1 80 6353 60 36 9 Customer Service Information Software Image Capture Kit PC Board amp LISCAP Software 1 80 6247 39 LISCAP NT Driver 1 18 1132 58 ImageM aster TotalL ab 1 18 1132 28 37 Amersham e Y Biosciences
10. appropriate PRESETS key 6 Print the image on the thermal printer by pressing the PRINT key on the printer or send the digital image to an external computer by pressing the DATA key Standard Settings Gel staining method EtBr Coomassie Silver M anual controls Light tray UV White White Lamp control mode Trans Reflector Trans Reflect or Trans Lens assembly Filter Orange Yellow Blue Aperture f stop 4 22 16 Electronic controls Camera control panel Preset key 1 2 5 Camera settings Integration time s b 1 8 0 07 0 2 Gain 0 0 0 B Lev 6 6 6 Gamma 0 45 0 45 0 45 The numbered keys on the camera control panel are factory set shortcut keys that set the electronic video settings to the optimal settings for the gel type These keys can be programmed to other settings The optimal integration time for each system is determined at the factory Refer to the sheet that accompanied your ImageM aster VDS for initial settings 4 Quick Reference Guidelines U sing the ImageM aster VDS as a quantitative tool The most accurate method to quantitate an unknown sample is to load multiple aliquots of several dilutions of the sample alternating lanes with well characterized standards which are as similar as possible to the unknown in composition and size Comparing samples side by side with standards on the same gel minimizes effects of uneven illumination differences in gel concentration pH stain concentration temperature an
11. be properly grounded to protect both personnel and the equipment All devices must be turned off before connecting to any other device such as a computer M aking connections with devices turned on may damage both devices Save the documentation showing the optimized factory set parameters for your system Refer to these reference parameters should the system require resetting The camera must be initialized every time the unit is turned on or for best performance at least once every 24 hours See section 5 1 Store film in a cool place away from sunlight If the thermal printer will not be used for an extended period more than one month move the thermal head release lever down to the RELEASE position see Figure 10 to prevent deformation of the rubber roller 1 Gel Documentation System Function and Description Video monitor Camera control panel ImageMaster VDS The top compartment contains the camera and lens assembly the lamp control panel and the secondary power switch The bottom compartment allows access to the sample Printer Removable transilluminator sample tray Lamps Control indicator see Section 7 2 Replacing lamps for a full description The UV filter tray is standard the white light tray is optional Figure 1 ImageMaster VDS system main components 2 Assembly and Installati
12. ch on the lamp control panel continuously Adjust the zoom and focus until the image is properly sized and sharp Close the compartment door once adjustments are final Select the final imaging light mode TRANS or REFLECT Close all doors The image should appear on the monitor Fine tune the electronic controls using the camera control panel The recommended starting point settings are B lev 6 Gain 0 Gamma 0 45 for output to the printer or 1 0 for output to computer Unless the settings have been reprogrammed PRESET 2 is factory set for optimal white light transillumination and PRESET 5 is factory set for optimal white light reflectance First optimize aperture and time settings and if further adjustment is required try incrementally increasing the gain default 0 or decreasing the B lev default 6 N ote Changing the B Lev setting may reduce the dynamic range for instance decreasing the value removes part of the signal This could be useful in removing background noise Once the image is optimized press the STILL key to hold and display on the monitor the image currently stored in camera memory Note Pressing the STILL key stores the image so that the sample can be removed from the UV tray to minimize photoknicking Pressing the STILL key stores the information but is not necessary to send the image to the printer or computer 19 5 Operating Instructions N ote If no activity is detected for 10 mi
13. cover by pressing the PUSH button on the upper left corner of the cover To rewind the film press the FUNC key If necessary apply gentle pressure to the film drum to help feed the film at the proper rate TheLED will indicate PrU Printer unwind Once the film edge is free release the FUNC key If loading a new roll of film Go to step 3 of section 7 4 Loading a roll of film If cleaning the printer roller go to step 2 of section 7 5 Rubber roller If cleaning the thermal head go to step 2 of section 7 5 Thermal head Clearing a film jam Important Allow the printer to rewind the film never pull the film out while the printer power isON or while the lever isin the PRINT position 1 The VDS mains power switch must be on 1 the secondary power switch must be switched to ON and the printer power switch must be ON Release the printer cover by pressing the Push button on the upper left corner Inspect the spool for proper placement the film path for obstructions and the film for signs of bunching or buckling To remove a damaged section of film lower the thermal head release lever into the RELEASE position and allow the roller to advance the film into the stacking tray If the printer is unable to feed the film try rewinding the film a Return the lever to the PRINT position and switch the printer power OFF b Simultaneously hold down both the PRINT and FUNC keys and turn the printer powe
14. ct the Technical Service Department for advice 21 6 Troubleshooting 22 M onitor and lamp variables 1 Check that the monitor is displaying the image currently in camera memory the STILL indicator lamp should be OFF 2 Lamps automatically shut off after 10 minutes of inactivity To reactivate open and shut either compartment door 3 Check that all lamps are functioning Replace as required see section 7 2 Sample variables 1 M ake sure the sample is completely flat against the sample tray 2 Flatten dry samples by placing a glass plate over them or placing weighted strips along the side 3 Remove any bubbles under wet gels Printer No film emerges upon pressing the print key 1 Turn the printer power OFF Check that film is properly loaded 2 If the printer is out of film reload See section 7 4 Printer output is spotted 1 Send a cleaning sheet through the printer to clean the thermal head after every 100 prints See section 7 6 Film jams 1 Check that the stacking tray is properly installed To install slide it into the stacking tray slot holding the tray level it should snap into place with a click Film quality 1 Check printer settings there are two film types and the proper setting must be programmed See section 7 4 step 9 2 Store film in a cool dark place to preserve film quality Error messages 1 R eset the printer by turning the printer power switch off and then on 2 R efer t
15. d other experimental variation all of which may affect fluorescence or absorbance by the samples M easuring at several concentrations assures that some of the samples will fall in the linear region of the system response To assure consistency from gel to gel and day to day establish and adhere to a well defined procedure for gel and sample preparation standard characterization sample and standard loading electrophoresis conditions voltage or current staining and destaining position on the light table image magnification and camera settings for both the lens and electronic parameters 15 5 Operating Instructions 5 Operating Instructions 5 1 Preliminary steps Place the sample and install the sample tray Note The bottom compartment door must be open in order to install or remove the sample tray 16 1 O pen the bottom compartment door to gain access to the sample tray Select the required tray The basic system includes a 312 nm UV transilluminator tray An optional white light sample tray can be ordered separately Place the sample on the tray It is easiest to place the sample before installing the tray but the sample can also be placed with the tray already in place If the gel is wet take care to not trap any air pockets or bubbles beneath it as they will interfere with image clarity If the gel is dry make sure the surface is flat This can be accomplished by placing a glass plate over it or lay
16. ermal head release lever down to the RELEASE position Lift the film roll out of the holder rails Remove the flanges from both ends of the spool and insert them into the new roll of film Seat the ready film Figure 11 Loading a new roll of film spool into the holder rails Once the spool seats the rubber roller l will start rotating Guide the film to the back of the compartment and gently press the middle of the spool just until the film starts to selffeed debiser inise Figure 12 Feeding the film through the roller 7 Care and Maintenance Allow the film to feed until it emerges at the stacking tray and then stop the roller by lifting the thermal head release lever into the PRINT position Close the front cover Optional Reset the counter to zero by simultaneously pressing the A and PRINT keys Each film roll will produce 170 records at the default 4 3 aspect ratio setting Figure 13 Closing the printer cover If you are switching to a different film type change two settings as follows Film select setting 9 1 a When the printer is in normal or standby state showing the print number press the W key and then also the FUNC key This will switch to a submenu in which 6 parameters can be set 9 1 6 Forward through the menu 5 steps by pressing the FUNC key until either LCr required setting for Super VDS film or TFF required setting for V DS film appears Press either W key
17. feature can be defeated for access to the manual camera controls in the top compartment by pressing the BY PASS button As long as this button is depressed the chosen lamps light Secondary power switch Both the mains power switch in the back and this switch must be on Under normal use leave the mains 7 power on and control power to the an AL unit with this switch Lighting mode selector switch 4REFLECT TRANS z A Bypass option bypasses the safety eer interlock that turns off lamps when BYPASS compartment doors are opened or the sample tray is removed Use with care to avoid UV light exposure Figure 3 Lamp control panel 3 System Component Close up 3 2 Video camera manual controls The highly sensitive CCD video camera captures and stores the image in 256 levels of gray and displays it on the monitor This image is manipulated both manually by adjustments to the lens assembly described in this section and electronically by specifying settings using the camera control panel described in the next section The three lens adjustments are aperture zoom and focus Aperture The aperture setting controls the amount of light that enters the CCD The aperture also referred to as the f stop ranges from f 1 2 to f 22 Settings i
18. hen BY PASS is pressed Wear protective clothing and UV blocking glasses to prevent exposure to UV light System Component Close up Lamp controls The top compartment houses the lamp control panel described in this section and the video camera CCD mount with the lens assembly described in the next section To turn on the lamps the sample tray must be in place and all doors must be closed Turn on the mains power switch at the rear of the unit on and then turn on the secondary power switch on the lamp control panel Depress the ON side of the switch A Lamps Control indicator to the right of the printer indicates lamp status OK indicates that all lamps are in good working order See the Section 7 2 Replacing lamps for a key to signals indicating lamp malfunction Lighting modes Choose TRANS for transillumination or lighting from below The UV tray automatically powers the UV lamps for UV transmittance and the white tray automatically lights the white lamps for white light transmittance Choose REFLECT for reflective lighting or lighting from above This mode powers a single white lamp located at the top of the chamber It is useful for 1 Focusing a sample that will be transilluminated with UV because reduced sample exposure to UV light reduces photoknicking and 2 imaging opaque media such as membranes UV light safety O pening any door or removing the sample tray switches off all lamps This safety
19. hite Auto selects the lightest area and compares it to a pre set white level for optimum contrast Selectable increments range from 0 9 The 0 setting shows raw data and is recommended especially for data sent to a computer for analysis Try adjusting the time and aperture settings before adjusting the gain or black level controls the dynamic range of the voltage signal which affects CCD sensitivity Auto selects the darkest area and compares it to a preset black level for optimum contrast The recommended starting setting for both UV and white light imaging is 6 Selectable increments range from 1 9 Any setting other than 6 decreases the sensitivity of the collected information but increases the contrast Controls the linearity of the CCD The gamma correction setting should be set to 0 45 for viewing the sample on the monitor and printing the image with the thermal printer It should be set to 1 0 if the image is sent to a computer 11 3 System Component Close up 12 3 5 Printer Printer parameters are preset for ImageM aster V DS system applications and Super VDS film is already loaded To print an image press the PRINT key Film types and film loading Two types of film are available The high grade Super VDS film is installed at the factory and the printer is set to process this thermally fixed film The regular grade film is UV fixed so the Film Select setting must be adjusted if sw
20. icate error condition 1 and an audible beep will sound 3 M ake sure the thermal head release lever is in the PRINT position 4 N ote the instructions on the cleaning sheet Do not touch the cleaning section of the sheet be sure to insert the sheet with the cleaning surface facing up the printed side of the sheet will be against the rubber roller and the matte side will contact the thermal head and take care to align the sheet To feed properly the positioning line printed on the cleaning sheet should align along the entire length of the edge just above the rubber roller Proper alignment requires that the sheet be even so that it won t skew as it feeds and that the sheet be making contact with the rubber roller for a firm grip gently edge the sheet down as far it will go 7 Care and Maintenance Simultaneously press the FUNC and A keys The cleaning sheet will advance but not pass all the way through As the sheet advances the LED will switch from indicating error 1 to indicating cleaning mode C3 If the cleaning sheet does not begin to feed move the thermal head release lever to the RELEASE position and reposition it When the cleaning sheet begins to enter the stacking tray an audible beep will sound and the LED will display cleaning mode CL2 M ove the thermal head release lever to the RELEASE position The printer will then eject the cleaning sheet and the LED will first display cleaning mode CL1 and then error 1 for abou
21. ifications Printer Transilluminator sample trays Recording system Fuji thermal film N umber of prints Resolution Gray levels Imaging mode Aspect ratio Image memory Analytical area Working area Transilluminator lamps PC options Image capture board LISCAP image capture software LISCAP NT Driver Image capture kit ImageM aster TotalL ab Product C ertifications Safety Emissions Immunity M arking Thermal VDS Film 101 mm W x 14 m L Super VDS Film 101 mm W x 14 m L Print cycle time 17 20 s 170 standard size portrait 1 1 140 long size landscape 4 3 Pixels 6 73 dots mm elements 13 46 dots mm Selectable 256 64 32 or 16 Selectable positive or negative mode Selectable 1 1 or 4 3 O ne frame image memory incorporated 18 x 23cm 21x 25cm UV lamps 8 W 312 nm 4 lamps White light lamps 8 W 5 lamps 8 bit board to allow transfer of the digital camera image data to an IBM PC type computer Data image capture software for the PC operating under Windows image capture board required Drivers for the Image capture kit under Windows NT 4 0 only Data capture board and LISCAP software Data capture and analysis software for the PC operating under Windows 95 98 and NT 4 0 image capture board required EN 60950 EN 55022 or equivalent documented standard IEC 801 2 IEC 801 3 IEC 801 4 CE mark This declaration of conformity is only valid for the instrument
22. ing weighted strips along the sides To install the tray Position it with the front edge slightly tilted up so that the sample tray rollers engage with the roller tracks Insert the drawer rollers into the track and lower the tray slightly The tray functions like a drawer close it completely 5 Operating Instructions Important The camera must be initialized every time the system is turned on in order for the CCD to be properly mapped N ote If an external computer is connected to the ImageM aster VDS system first initialize the camera and then turn on the computer 5 2 Caution Exposure to UV light can cause serious damage to eyes and skin Cover your skin and wear UV blocking glasses Initialize the camera 1 Important The camera must be initialized every time the system is turned on or a minimum of once every 24 hours in order for the CCD to be properly mapped The CCD requires complete darkness for this operation The recommended way to accomplish this is to install the lens cap Alternatively turn the aperture control to f 22 Install a sample tray close all doors and turn the mains power on Initialization requires 10 15 seconds During initialization Please wait for initialization appears on the monitor and the Lamps Control indicator flashes the signal OK If the message Please make sure all the doors are closed appears instead close all the doors to continue
23. itching film type Section 7 4 H andling film describes how to load new film and step 9 describes changing the Film Select setting Latch to open and close the front panel for film loading and roller cleaning RE FUJIFILM D _ Printer power switch THERMAL IMAGING SYSTEM FTI 500 EI a PUSH zs A FUNC REPRINT de N A JA a Xx i x Se The LED displays print x number or the printer settings if using the FUNC keys Slide the stacking tray into this slot holding the tray level When properly installed the tray snaps into place with a click Figure 6 Thermal printer functions r 4 7 Press the PRINT key for a print of the image displayed on the monitor Press the REPRINT key for additional copies of the image displayed on the monitor The same ID information is printed on each reprint The FUNC key accesses all printer settings The arrow keys increase or decrease the value displayed on the LED 3 System Component Close up Printer Printer function quick reference To print press the PRINT key Prints are numbered sequentially and the current print number displays when the printer is in standby ready mode pressing the PRINT key again increases the print number by 1 To print an identical image multiple times press the REPRINT key This produces the last image stored in printer memory which may be different fro
24. m the image on the monitor if the camera has collected data of a new image Pressing PRINT and the A key resets the print number to 0 notes Turning the power off clears the printer memory Allow about 30 seconds between prints for optimum print quality During the printing process the printer is not available to execute additional commands The LED will display P If the printer will not be used for one month or more release the thermal head to prevent deformation of the rubber roller See section 7 4 Loading a roll of film step 3 and also Figure 10 To clear a film jam refer to section 7 5 When the roll is nearly used 10 prints or fewer remaining a printer alarm sounds and the LED displays END See section 7 4 for film loading instructions If using the regular grade UV fixed film the UV fixing lamp stays lit even when the printer is not printing If the lamp goes off because the unit has not been activated for 2 hours turn the printer off then on to reset the lamp The factory settings for the printer are listed in Section 7 7 for 13 4 Quick Reference Guidelines 14 4 Quick Reference Guidelines 1 Place the sample on the appropriate sample tray and install the tray 2 Initialize the camera 3 Size and focus the image in REFLECT mode 4 Select the light mode TRANS for transillumination or REFLECT for lighting from above 5 Set the electronic camera controls Or select the
25. n the low range f 1 2 4 refer to the widest aperture allowing more light to enter M ore light is required for fluorescent imaging and other low light level applications Higher settings above f 8 narrow the aperture allowing less light to enter Less light is required for white light imaging Higher aperture settings generally require longer exposure times see the TIM E menu option section 3 4 Tabulated in the Quick Reference Guidelines section 4 are starting point apertures for imaging different gel staining methods Zoom The zoom adjusts the focal length from 8 48 mm Increasing the focal length enlarges the image Size the image to fill the monitor Focus The focus control sharpens the image N ote the 2 0 closeup diopter lens must be in place for proper focusing Aperture adjustment f 1 2 22 Zoom adjustment 8 48 mm Focus 2X diopter lens Color filter enhances the image use orange for UV imaging or the appropriate filter for your application NVdvf wwgg df 3SO19 Nvdv r WSS Figure 4 Lens assembly in the top compartment 3 System Component Close up 3 3 Video camera electronic controls Note Recommended settings for the DATA function gamma 1 0 B lev 6 gain 0 Adjust the aperture and time parameters to optimize the image 10 The camera control panel is located under the monitor Each function is described below Further manipulation options are described in section
26. nutes the lamps automatically shut off To reactivate open and close either door The image may be captured by pressing STILL or DATA before the lamps switch off 20 Print the image by pressing the PRINT key on the thermal printer While printing the printer displays P and does not accept further commands Once the print number appears the printer is ready to produce another print Note The printer prints the image on the monitor at the time the PRINT key is pressed For a record of the video settings press PRINT while the setting menu is on the monitor T he setting menu can be recalled by pressing any cursor key To send theimage to a computer running the optional LISCAP software press the DATA key Note The video settings cannot be sent to the computer 6 Troubleshooting Troubleshooting No indicator lamps light and the monitor remains blank Check the power source and connections 1 Check that the power cord is plugged into a working electrical outlet 2 Check the power cables for loose connections 3 Check the fuses Replace if they are damaged Lamps don t turn on 1 Check that both the mains power switch on the back panel and the secondary power switch in front top compartment areON 2 If the Lamps Control indicator shows a lamp failure replace the indicated lamp See Section 7 2 Replacing lamps 3 Lamps automatically shut off after 2 minutes of inactivity To reactivate open and sh
27. o the separate FUJI film Thermal Imager Instructions accompanying the ImageM aster VDS system The error code key is on page 16 7 Care and Maintenance 7 7 1 7 2 Care and Maintenance Cleaning U nit body Use a soft cloth dampened with water or a mild cleaning solution to clean the cabinet and display Sample trays Wipethe sample tray after each use to remove all residual materials Use only a damp cloth and a mild cleaning solution Do not mar the sample tray surface by cutting gel on it or scraping gel away Lens and lens filters The CCD camera mount closeup diopter lens zoom lens and the filter must all be clean because any foreign material will reduce image quality Periodically check for dust and dirt and clean with only professional quality lens cleaner and lens cleaning paper N ever over tighten any lenses to prevent moving the camera mount which may result in altering the plane of focus Replacing lamps Lamps Control indicator The Lamps Control indicator electronically monitors lamp output A lamp typically provides 500 hours of service A lamp failure is indicated by both a single lit lamp under the Lower and Upper row and by a row of lamps lit horizontally in the L1 to L4 field An example is illustrated below In case of failure replace the indicated lamp Refers to the ultraviolet UV and Refers to the white light WL white light WL lamps in the lamp in the upper lower compar
28. on 2 Note H andles ordered separately can be installed to facilitate moving the unit Assembly and Installation The ImageM aster VDS system is assembled with the following exceptions the video camera lens assembly the printer film and the power cord Place the unit on a vibration free level surface in an area away from direct sunlight and with relatively constant ambient temperature and humidity Video camera lens assembly The lens assembly comprises a 2X diopter lens a color filter and controls for the aperture zoom and focus See section 3 2 for component functions To install the lens assembly l Note The lens assembly includes an orange filter when shipped If the unit is to be used for white light photography of stained gels unscrew the orange filter and install a yellow or blue filter ordered separately O pen both the top and bottom compartments for better access Unscrew the protective screw cap from the CCD video camera head if present Screw the lens assembly into the CCD video camera head we recommend reaching up through the sample compartment so that you can support the lens assembly with one hand while screwing it into the video camera mount with the other hand N ever force the assembly into the thread finger tight installation is enough O ver tightening can alter the plane of focus by changing the camera mount position Leave the lens cap in place until after camera initializati
29. on is complete Load film into the thermal printer If no film is loaded into the thermal printer see Section 7 4 steps 1 8 to install the provided film 2 Assembly and Installation Power connection 1 Important Verify that the AC power outlet is properly grounded If the outlet is not grounded connect the ground terminal to an electrical earth ground 2 Important Check the voltage specification near the fuse holder for the voltage setting T he setting must match the voltage of the power supplied to the unit 3 Plug the power cable into the plug receptacle in the unit and then plug the connector into the power socket Fan vents 2 Serial number and specification panel D type 25 pin connector for computer cable ___ BNC connector Jj only the right one is functional e Fuse holder 1 Mains power On Off S switch 0 off I ly Power cord receptacle Ne RIE Figure 2 Rear panel The rear panel houses the mains power switch cable connectors and fuse holder 3 System Component Close up 3 3 1 N ote To prolong lamp life all lamps automatically switch off after 10 minutes of no detectable activity Lamps are re activated as soon as either door is opened and closed Caution If TRANS is selected and the UV tray is installed the UV lamps will light w
30. or the A key to toggle between the two choices Press and hold the FUNC key for longer than 0 5 s to set the current value and exit Tone setting 9 2 a When the printer is in normal or standby state showing the print number press the FUNC key to enter the Print mode submenu 9 2 6 Forward through the menu 3 steps by pressing the FUNC key until either T 3 recommended setting for Super VDS film or T7 recommended setting for VDS film appears Please note that the setting that results in the closest approximation of the image on the monitor may vary slightly from these values 9 2 c Press either amp key or the W key to scroll through the tone setting options until the desired setting appears Press and hold the FUNC key for longer than 0 5 s to set the current value and exit 27 7 Care and Maintenance N ote It is advisable to clean the roller after a jam to remove particles that may streak the film See section 7 5 28 Removing a roll of film Important Allow the printer to rewind the film never pull the film out while the printer power isON or while the lever isin the PRINT position 1 Set the printer to service mode The ImageM aster V DS mains power switch must be ON 1 the secondary power switch must beON and the printer power switch must be OFF Simultaneously hold down both the PRINT and FUNC keys and turn the printer power switch ON TheLED will indicate SEV Release the printer
31. ortant Turn the mains and secondary power switches off and disconnect the power cord from the mains power Remove the sample tray and open the lower compartment door N ote the four lamp pairs each pair labeled one UV lamp and one white light lamp Remove the malfunctioning lamp by carefully rotating it about Aturn counter clockwise until the pins align vertically Carefully pull the lamp upward To insert the new lamp hold it so that the metal pins align vertically Place the lamp in the sockets at each end of the unit and lightly push it into place Rotate the tube about turn clockwise until it locks into place 7 Care and Maintenance 7 3 Replacing fuses Fuses protect equipment by disconnecting loads too large for the instrument s circuit design For continued protection replace only with fuse of the specified voltage and current ratings The fuse holder above the mains power cord receptacle holds two 250 V fuses 110 120 V 60 Hz US model fuses 2 F 4A 250V 220 240 V 50 Hz European model fuses 2 F 2 5A 250V 100 V 50 60 Hz Japanese model fuses 2 F 4A 250V 1 Caution Turn the mains and secondary power switches off and detach the power cord before replacing fuses 2 Insert a small flat blade screwdriver into the slot on the fuse holder depress and pull the holder door forward 3 Pull the fuse out of its holder and inspect If the fuse element is burned or broken replace the fuse with an iden
32. plays it on the monitor O nce the STILL key is pressed the indicator lamp lights and the camera stops collecting video information To resume collecting image data press the STILL key again The STILL indicator lamp is not lit when the image on the monitor is displaying real time information 3 System Component Close up 3 4 Menu options displayed on the monitor As long as the camera is active i e it is sending images to the monitor because the STILL key has not been pressed camera settings can be manipulated to optimize the image before sending it to the printer or the optional software Setting guidelines are listed in the Quick Reference Guidelines section 4 Setting descriptions follow SET TIM E S GAIN B LEV Gamma Programs the four settings described below into a program memory keys 1 5 Once parameters are selected move the cursor to set and press the desired memory location one of keys 1 5 for 5 seconds Shows the camera integration time or exposure in seconds There are 70 selectable times ranging from 1 10 000 to 10 s The time value wraps around increasing or decreasing by a small increment except at the end values where increasing from 10 s results in the lowest value 1 10 000 and decreasing from the lowest value results in 10 s or the highest value W hen adjusting the time value keeping the arrow key depressed accelerates scrolling through the values Controls the level of w
33. r switch ON TheLED will indicate SEV N ote If a section of film is stuck in the exit pressing the W key in the SEV mode will trim it off 7 Care and Maintenance 7 5 c To rewind the film apply gentle pressure to the film drum to help feed the film at the proper rate and then press the FUNC key to start rewinding The LED will indicate PrU Printer unwind Once the film is free release the FUNC key M ove the thermal head release lever down to the RELEASE position Reload the film according to instructions in section 7 4 Loading a roll of film steps 5 to 7 If the roller is still unable to feed the damaged film turn the power to the printer OFF and lower the lever into the RELEASE position Lift the roll of film out and slowly but firmly pull the film free of the roller O nce the film is free cut off the damaged section It may be easier to reload the film if you angle the cut slightly Turn the printer power back ON Reload the film according to instructions in section 7 4 Loading a roll of film steps 5 to 7 Cleaning the printer Rubber roller White vertical lines or black spots on your prints may indicate that the rubber roller and or the thermal head require cleaning If cleaning doesn t improve the photo quality contact a qualified service technician to replace the thermal head or roller 1 Remove the film according to the instruction in section 7 4 Removing a roll of film Figure
34. read APBiotech follow the procedure below For additional codes to program your preferred text refer to the FUJI manual a Press the FUNC key while holding down both the 4 and Codes for a APBiotech Vv keys to access FUNCTION label on each print M ode 3 Then select S5 FUNCTION Mode5 by Character position character pressing the A key twice pode b Press the FUNC key two times to access the ID character set The position 0 and character 46 code for F in FUJI FILM will appear 046 41 50 42 69 67 74 65 63 68 c In this position usethe A and W keys to select the desired character code for the first 0 position For A the code should be 041 as shown in the table Press the FUN C key to accept The next set of character codes automatically appears The table lists all position and character codes required to program A PBiotech 0 YNQDQDUIASWN_O A P B i o t e t h d Press the FUN C key for more than 0 5 s to set the selected values and exit Check that the film type setting matches the film type in the printer section 7 4 step 9 To match the print to the monitor image follow the procedures in section 5 2 for UV transillumination imaging or section 5 3 for white light imaging 33 8 Specifications 34 8 Specifications ImageM aster VD S system Voltage Power consumption O perating environment System timer 1 0 ports Dimensions
35. s a 312 nm ultraviolet UV transilluminator sample tray and an orange lens filter is intended for documentation of ethidium bromide stained agarose and polyacrylamide gels O ptional accessories such as a white light sample tray and a blue or yellow lens filter allow documentation of Coomassie blue and silver stained gels as well as membranes films blots and autoradiographs The sample area is 21x25 cm This accommodates the developed area of one full size M ultiphor gel 8 mini gels or up to 15 PhastGel gels The imaging system comprises a highly sensitive charged coupled device CCD camera connected to a monitor which allows viewing of the image and manipulation of camera controls before the image is printed This imaging system is contained in a free standing dark room that includes a UV transilluminator as well as a white light source Once the image is optimized it can be printed by the integral thermal printer Two grades of film are available the higher grade Super VDS film is thermally activated and fixed and the regular VDS film is thermally activated and UV light fixed Both use a polymer emulsion technology that produces a sharp image on tough plastic and both have an image life of seven years under the proper storage conditions If the optional LISCAP image capture software and PC board are installed on your PC computer the digitized image can be transferred to your PC The digital image is then available for analysis
36. t 30 seconds If the sheet becomes stuck because it engaged with the roller too late turn the power to the printer OFF and lower the thermal head release lever into the RELEASE position Slowly but firmly pull the sheet free Go back to step 3 Repeat steps 3 to 5 as often as required To reload the film see section 7 4 Loading a roll of film steps 5 to 7 31 7 Care and Maintenance 32 7 6 Menu available by pressing FUNC key only Menu available by pressing both the FUNC PRINT mode and the W key simultaneously Brightness box FUNCTION Mode 1 Contrast co Output THr Tone T3 Super VDS film or Enhance EoF T7 Regular VDS film H size 50 Gain select Gin Format F10 Gain G28 H posi HOC V posi UOE The available range for these settings is 9 to 9 However 0 is the default and nominal setting and settings outside the 3 to 3 range are not recommended Menu available by pressing both the FUNC and Menu available by pressing the FUNC A the A key simultaneously FUNCTION Mode 2 and W keys simultaneously Aspect A43 First set the LED display for S3 with the Int Noint in A and Y keys and then press FUNC to Odd Even odd access FUNCTION Mode 3 Mono color Cro ar Direction do Signal select S_A A Film select LCr Super VDS film or ros Neda PoS TFE Regular VDS fil Film cutsize C_n Regular ilm Cutter Con Mirror obu Gradation G8 Menu available by pressing the FUNC A and Menu available by pressing the FUNC A V
37. the thermal printer While printing the printer displays P and does not accept further commands Once the print number appears the printer is ready to produce another print Note The printer prints the image on the monitor at the time the PRINT key is pressed For a record of the video settings press PRINT while the setting menu is on the monitor The setting menu can be recalled by pressing any cursor key To send the image to a computer running the optional LISCAP software press the DATA key Note The video settings cannot be sent to the computer 5 Operating Instructions 5 3 White light imaging procedure 1 2 3 4 5 6 N ote 7 Adjust only one parameter at a time and only by small increments 8 Follow the preliminary steps in section 5 1 M ake sure you have chosen the white sample tray that the sample is in place and that the tray is properly installed Verify that the video camera is initialized and that the appropriate filter is installed on the lens assembly Set the camera aperture in the smaller aperture range starting with f 22 for reflectance and slightly larger lower f stop for transillumination Switch the main lamp control switch on the lamp control panel to ON and choose REFLECT for initial image focusing Size and focus the image The top compartment door must remain open while you adjust the camera zoom and focus To switch on the light while the door is open you must press the BY PASS swit
38. tical type If the fuse appears to be intact check it with an ohmmeter 4 Insert a good fuse into the holder and press the holder door shut Fuse holder GaN Figure 9 Mains power module 25 7 Care and Maintenance 7 4 Note Keep film away from direct sunlight during loading Always store film in a cool dark place Note Cleaning the thermal head after each roll of film is a recomme nded preventative maintenance measure See section 7 5 Cleaning the printer Thermal head for the procedure which is easily performed when changing film 26 Handling film Loading a roll of film This section describes how to install a new roll of film when no film is currently installed or when the film roll is empty To switch to a different type of film first remove the installed film by following the instructions in the next subsection Removing a roll of film Important N ote the type of film you are installing Super VDS film is included with the unit and the printer is originally set to LCr to process it R egular VDS film can be ordered and if used the setting must be changed to TFF see step 9 1 The VDS mains power switch must be on I the secondary power switch must be switched to ON and the printer power switch must be on Release the printer cover by pressing the Push button on the upper left corner Figure 10 Removing an empty roll of film M ove the th
39. tment under the compartment behind the transilluminator camera lens assembly LOWER UPPER Example VV f y UV_ WL UV WL If this single lamp 20 O lights as well as the row of lamps to the _ left of Ly replace the _ L1 L2 L through Ly refers to L3 lamp sets numbered UV lamp in the lower L4 from the front to the rear compartment at the of the compartment rear of the unit LAMPS CONTROL Figure 8 Lamps Control indicator 23 7 Care and Maintenance Note If one or more lamps are burned out due to age all lamps should be replaced at the same time to ensure even light intensity 24 UV lamps Required 8 W 312 nm UV lamp Code no 80 6249 45 To replace a lamp 1 Important Turn the mains and secondary power switches off and disconnect the power cord from the mains power Remove the sample tray and open the lower compartment door N ote the four lamp pairs each pair labeled one UV lamp and one white light lamp Remove the malfunctioning lamp by carefully rotating it about turn counter clockwise until the pins align vertically Carefully pull the lamp upward To insert the new lamp hold it so that the metal pins align vertically Place the lamp in the sockets at each end of the unit and lightly push it into place Rotate the tube about turn clockwise until it locks into place White light lamps Required 8 W white light lamp Code no 80 6249 29 To replace a lamp 1 Imp
40. ut either compartment door Power indicator lamps are lit but the monitor remains blank 1 Check that the sample tray is properly installed 2 Check that both doors are closed and the lens cap was removed from the lens assembly after initialization 3 Check the UV or white light lamps by pressing BY PASS 4 Increase the aperture to allow more light to enter the camera Try Opening it completely f 1 2 then decreasing the aperture incrementally as required 5 The camera must be initialized each time the mains power is turned on and at least once every 24 hours To initialize be sure the lens cap is in place or set the aperture to f 22 and close all the doors See section 5 1 U nclear or no image on the monitor Camera variables 1 Initialize the camera by switching the mains power off and then on see section 5 1 No light must enter the lens during initialization so be sure the lens cap is in place or that the aperture is set to f 22 and all doors are closed Be sure to remove the lens cap after initialization is complete 2 For the zoom lens to be able to focus the close up diopter lens 2X must be in place See section 3 2 for a lens assembly diagram 3 M ake sure the camera lens filter and CCD camera mount are free of foreign materials Clean with lens paper if required 4 If the camera mount position has changed due for example to over tightening the camera lens the plane of focus may be out of range Conta
41. v keys simultaneously and W keys simultaneously First set the LED display for S4 with the A and First set the LED display for S5 with the V keys and then press FUNC to access A and V keys and then press FUNC to FUNCTION Mode 4 access FUNCTION Mode 5 LED display select d50 ID print Pon Print no print Con ID character set 050 T H temp print ToF Note EPDT 9A8 The 16 available characters are programmed VSDT 5Cd i ee n Filter select 5F1 to spel Amersham Biosciences at the factory To reprogram see the next section or the Fuji Thermal Imager instructions Printer pre sets Printer parameters are preset for ImageM aster V DS system applications The factory set parameters for the system are listed below for your reference Note The settings in bold are for Super VDS film See Section 7 4 Loading a roll of film step 9 for instructions on how to change film type settings Restoring default printer parameters If the printer settings arein an unknown state restore default parameters according to the instructions below For a full description of all printer options and settings see the Fuji Thermal Imager instructions accompanying your ImageM aster VDS system 1 To initialize all printer parameters hold down both the W and PRINT keys while turning the printer mains power switch ON 7 Care and Maintenance After printer initialization step 1 each print will be labeled FUJI FILM To program the 16 character label to
42. when it is used in laboratory locations used as delivered from Amersham Biosciences except for alterations described in the User M anual and connected to other CE labeled instruments or products recommended or approved by Amersham Biosciences 35 9 Customer Service Information 9 Customer Service Information 9 1 Technical Service and Repair Amersham Biosciences offers complete technical support for all our products If you have any questions about how to use this product or would like to arrange to repair it please call or fax your local Amersham Biosciences representative Important Request a copy of the Amersham Biosciences H ealth and Safety Declaration Form before returning the item No items can be accepted for servicing or return unless this form is properly completed 9 2 Ordering information ImageM aster VD S system Qty Codeno Each unit includes M ain body UV tray 8 48 mm zoom lens orange filter close up diopter lens operation manual power cord roll high grade film loaded in printer ImageM aster VDS DU 115 V 60Hz 1 80 6246 82 ImageM aster VDS DE 230 V 50Hz 1 80 6247 01 ImageM aster VDS DJ 100 V 50 60Hz 1 80 6247 20 H andles for moving the ImageM aster VDS unit 2 80 6382 29 Fuses F 4A 250V 5x20 mm 2 80 6111 54 Fuses F 2 5A 250V 5x20 mm 2 80 6406 23 Sample trays and lamps UV filter sample tray 1 80 6248 15 W hite light filter sample tray 1 80 6248 34 Tube
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