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User manual - Miltenyi Biotec

1. 4 4 Main menu The Main menu is used to select and run dissociation programs for each ofthe eight tube positions Users have the flexibility to run multiple programs simultaneously or sequentially Samples can also be run individually The Main menu also reports the status of each tube position 4 4 1 Status of sample tube positions All possible statuses of tube positions are shown in the screen below and described in table 4 4 a Programs Date y 37C_m_tumor_01 19 Apr 2013 Figure 4 7 Overview of tube position statuses displayed in the Main menu 1 Free 2 Selected 3 Ready and Heater installed 4 Ready 5 Running 6 Done 7 Aborted 8 Stand by No tube is inserted The position is free to use Tube is inserted After inserting a tube the position is automatically assigned the status Selected Programs can only be designated to positions with the status Selected The tube position is ready A tube and a heater were inserted and a program was subsequently assigned by confirming the selection with OK The program may be commenced by pressing Start The program name and duration are displayed In this example program 37 C_m_tumor_01 will be completed within 5 minutes The tube position is ready A tube was inserted and a program was subsequently assigned by confirming the selection with OK The program may be commenced by pressing Start The program n
2. Miltenyi Biotec gentleMACS Octo Dissociator with Heaters User manual Version 1 Original instructions Copyright 2014 Miltenyi Biotec All rights reserved No part of this user manual may be reproduced stored in a retrieval system transmitted published or distributed in any form or by any means electronically mechanically by photocopying microfilming recording or otherwise without the prior written consent of Miltenyi Biotec GmbH however notwithstanding the foregoing the owners of the gentleMACS Octo Dissociator with Heaters may make copies solely for purposes of training personnel in the use and servicing of the unit within their business or organization Maximal care has been taken by Miltenyi Biotec GmbH in the preparation of this user manual However Miltenyi Biotec GmbH shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this document The information in this document is provided as is without warranty of any kind and is subject to change without notice gentleMACS and MACS are registered trademarks or trademarks of Miltenyi Biotec GmbH All other trademarks mentioned in this publication are the property oftheir respective owners and are used for identification purposes only Unless otherwise specifically indicated Miltenyi Biotec products and services are for rese
3. Dangers lectriques et incendie Les risques potentiels r sultant d appareils lectriques sont le choc lectrique le court circuit et la surchauffe Un choc lectrique peut entra ner de graves blessures corporelles ou m me la mort Un court circuit ou une surchauffe peuvent provoquer un incendie Des quipements lectriques peuvent mettre des tincelles pouvant enflammer des vapeurs ou mati res combustibles ce qui pourrait engendrer une explosion ou un incendie N utilisez pas cet appareil dans des endroits jug s dangereux par exemple dans un environnement riche en oxyg ne Le bo tier de l appareil est con u de fa on r duire les risques de choc lectrique de court circuit et de propagation du feu A l exception du Safety Shield et de la Trough n enlevez aucun couvercle et n y p n trez pas Seul un personnel autoris a le droit d enlever les autres couvercles N introduisez jamais un corps trangers travers d une fente l int rieure de l appareil N utilisez jamais l appareil lorsqu il est ouvert ou d mont e il est tomb ou qu il a t endommag e ila des pi ces endommag es ou abim es e ila des c bles lectriques endommag s un objet y est entr par les fentes de ventilation UN corps tranger a p n tr dans l appareil Si des flammes ou la fum e apparaissent coupez imm diatement l alimentation lectrique d branchez l appareil de la prise lectrique puis contactez
4. _setup_ 14 36 Clock Tube positions 1to8 Programs Date Info button Selected gt folder m_neoheart_01_01 C 19 Apr 2013 O Scroll buttons File E command Figure 4 4 Main menu of the gentleMACS Octo Dissociator with Heaters The Main menu is used to assign gentleMACS Programs to each tube position and run the respective process Programs can be loaded from a total of eight folders a Favorites folder a Miltenyi program folder a Templates folder and five User folders Please refer to section 4 4 for more details BE INSTRUMENT OPERATION Editor menu The Editor menu is used to create and edit programs Moreover programs are managed here Links to programs can be added to the Favorites folder and programs can be transferred among folders Please refer to section 4 5 for more details Programs Tube Date program_01 C 07 Apr 2011 O Available folders Figure 4 5 Editor menu of the gentleMACS Octo Dissociator with Heaters 61 Setup menu Instrument settings can be modified using the Setup menu This includes switching the LEDs on or off setting the time and date and naming User folders Moreover this menu provides information on software and program versions Please refer to section 4 6 for more details Main menu editor _ Device settings Device Set time settings a for settings Figure 4 6 Setup menu of the gentleMACS Octo Dissociator with Heaters
5. 200 cycles can be defined Table 4 5 Overview of program commands Tips and hints for user defined programs Do not exceed a maximum of 3 200 rounds per run when creating a new program Running higher numbers of rounds per run may result in damage of the sealing of the gentleMACS Tube Typically gentleMACS Programs consist of several spin commands each with a different speed and clockwise rotation Integration of short counter clockwise spin commands can increase the efficiency of the dissociation or homogenization Incubation periods typically run at 20 rpm A counter clockwise rotation has been benefical in many cases Creation of a user defined program 1 Highlight a particular User folder In this example the folder User_1 is selected Main menu Setup _ Programs Tube Date Favorites Miltenyi program_02 C 27 Jun 2013 O 2 Press Add A new_program file template will be displayed 3 Press the tube button to select a C Tube or M Tube 4 To select further options e g spin ramp temp loop and endloop press Add A dotted line will appear above the end command File new_program i name Switch between Tend Du C and M Tube ramp loop end loop Commands File commands Figure 4 8 New_program file template to create user defined program 5 Enter respective values and press OK 6 Press Add to insert the next command or Save to name and store the program 73 BE INSTRUMENT OPER
6. 27 Favorites program_02 C 27 Jun 201 U Miltenyi gt Note The duration of a gentleMACS Program is limited to 3 200 rounds per run For example a program that consists of one spin command at 4 000 rpm can run for a maximum of 48 seconds a program that consists of one spin command at 200 rpm can run for a maximum of 960 seconds 16 minutes A program that consists of one spin command at 20 rpm can run for a maximum of 160 minutes It is not recommended to run programs exceeding this limitation Miltenyi Biotec does not guarantee proper function of the instrument and disposables above this value However when saving a program exceeding 3 200 rpr a pop up screen will appear displaying a warning message and the current rpr value Press OK to save the program Press Abort to reedit the program 4 5 2 Editing programs To edit aprogram 1 Select the Templates or User folder containing the required program Note Files located in the Miltenyi and Favorites folders cannot be edited The Miltenyi folder contains gentleMACS Programs that have been optimized by Miltenyi Biotec for a variety of applications These programs cannot be altered The Favorites folder contains shortcuts to the original programs located in the Templates Miltenyi or User folders Note The template programs A to E provided by Miltenyi Biotec can be used to create a user defined program The newly created program has to be renamed and saved in one of the five
7. 58 Starting the instrument 59 Menu tabs 61 4 4 4 4 1 4 4 2 4 4 2 1 4 4 2 2 4 4 2 3 4 5 4 5 1 4 5 2 4 5 3 4 5 4 4 5 5 4 6 4 6 1 4 6 2 4 6 3 4 6 4 4 6 5 5 1 5 2 5 3 Main menu Status of sample tube positions Processing samples Running a single sample Running multiple samples simultaneously Running multiple samples sequentially Editor menu Creating a new program Editing programs Creating program shortcuts into Favorites Transfering programs to other folders Deleting programs Setup Set LED Set clock and date Set folder name Software version Serial number Maintenance Servicing Cleaning Instrument disposal Troubleshooting Technical specifications Technical support Limited warranty 62 62 64 64 67 68 72 72 78 79 80 82 83 83 83 83 84 84 85 85 85 87 89 93 97 99 CAUTION The gentleMACS Octo Dissociator with Heaters is designed for safe use when installed correctly operated by trained personnel operated in accordance with general safety practices and the instructions in this user manual The guidelines in this section explain the potential risks associated with operating this instrument and provide important supplemental safety information to minimize the risks Follow the instructions carefully to protect yourself others and the equipment from potential hazards and create a safe work environment Use this instrument only as specified by the manufactu
8. Biotec The gentleMACS Octo Dissociator with Heaters provides the perfect solution for the fast and easy preparation of single cell suspensions and homogenates from virtually any tissue M Samples are processed using unique gentleMACS Tubes with caps that have specially designed rotors and stators C Tubes provide gentle but efficient tissue dissociation yielding cell suspensions with high viability rate M Tubes provide thorough homogenization of tissues or cells Figure 1 1 The gentleMACS Octo Dissociator with Heaters equipped with gentleMACS Tubes and Heaters 49 50 The gentleMACS Octo Dissociator with Heaters provides the preparation of viable single cell suspensions by mechanical and enzymatic dissociation including incubations at 37 C the preparation of thorough tissue homogenates for subsequent extraction of sub cellular material such as total RNA DNA proteins organelles and pathogens e simultaneous or independent processing of up to eight samples even using different programs easy access to pre defined gentleMACS Programs which are optimized for particular applications the option to create user defined programs 1 2 Sample preparation for cell and molecular biology applications Two dedicated types of gentleMACS Tubes have been specially designed for the optimal preparation of samples for cell biology or molecular biology applications Purple C Tubes are typically used for the gentle prepara
9. OK to save the value new_program spin ramp loop 4 CH end loop end loop 6 end Note The temp command enables the heating of a sample to 37 C during a series of program steps e g for incubation purposes The incubation period will only include the steps between a temp ON and temp OFF command 11 Highlight the end command and press Add In this example two spin commands are added a counter clockwise spin at 20 rpm for 30 minutes and a clockwise spin at 3 000 rpm for 10 seconds Main menu Editor Setup 13 13 new_program ramp loop 5 A end loo spin 20 rpm 30 0 p temp 9 end 12 Highlight the end command and press Add Select temp and OFF from the pop up screen to switch off the heating function Press OK to save the value Main menu Editor Setup 13 13 new_program in 2000 rpm 1 in 2000 rpm 10 spin 5 end loop ramp loop end loop 10 end 13 Press Save to store the new program Enter the filename when prompted In this example the new program is named program_03 Main menu Editor setup 10 13 Note Program names can be a maximum of 16 characters Select CAP to alternate between upper and lower case characters 14 Select OK on the keypad to save the program The program will be automatically saved to the folder selected in step 1 In this example this is the folder User_1 77 BE INSTRUMENT OPERATION 78 Programs Tube Date 0 Lau program_01 Cc
10. Si spenga immediatamente lo strumento e lo si scolleghi dalla presa di corrente quindi si contatti il service provider Miltenyi Biotec o Miltenyi Biotec Technical Support Rischi elettrici e pericolo d incendio Possibili rischi derivanti da dispositivi elettrici includono scosse elettriche corti e surriscaldamenti Una scossa elettrica potrebbe causare gravi lesioni personali o perfino la morte Un corto elettrico o un surriscardamento potrebbero causare un incendio Le apparecchiature elettriche possono generare scintille che potrebbero incendiare vapori combustibili o materiali causando esplosioni o incendi Non si utilizzi lo strumento in aree classificate come postazioni pericolose p e in ambienti carichi di ossigeno Il corpo dello strumento stato realizzato per ridurre il rischio di scosse elettriche corti elettrici e la dispersione di fuoco Con eccezione per il Safety Shield e Trough non si rimuova o si apra alcun rivestimento Solo il personale autorizzato pu rimuovere le altre protezioni dello strumento Non si inserisca mai alcun oggetto esterno nelle aperture dello strumento Non si usi lo strumento se e aperto o smontato e caduto o danneggiato ha danni o ha parti rotte e hail cavo di alimentazione danneggiato un oggetto entrato nelle fessure di ventilazione e un oggetto esterno stato inserito nello strumento Se si vedono fiamme o fumo si escluda immediatamente l alimentazione si scollegh
11. User folders 2 Highlight the program of interest In this example program_03 in the folder User_1 is selected Programs Tube Date program_01 C 27 Jun 2013 O program_02 C 27 Jun 2013 O Miltenyi USB stick 3 Press Edit to open the respective program 4 Highlight the required step Edit the highlighted command by changing or adding required values rpm min sec or use the following command buttons to modify the program Delete to remove the highlighted step from the list Add to insert an additional step above the highlighted step Note Steps can be highlighted using the touchscreen or the scroll buttons 5 Select Save to store the modified file or Exit to discard all changes When selecting Save a pop up window displays the following options for selection Save to overwrite the existing program Save as to save the program with another name Cancel to abort the process program _ 45 3 Creating program shortcuts into Favorites Shortcuts to frequently used programs in the Miltenyi Templates or User folders can be created in the Favorites folder These shortcuts can be used to quickly access the required programs 1 Highlight the folder containing the required program 2 Checkthe corresponding checkbox to select the program Note that it is possible to select multiple programs Any program highlighted or not can be selected Main menu Programs Tube Date program_01 program_02 p
12. before installation or use If manual is missing obtain replacement from Miltenyi Biotec La cuve est marqu e avec les symboles de s curit suivants Marquage color sur les manchons Symbole de s curit Figure 1 Symbole de s curit sur la fosse du gentleMACS Octo Dissociator with Heaters Les manchons b n ficient d un marquage color Surface chauffante Symbole de s curit Figure 2 Symboles de s curit relatifs la partie chauffante du gentleMACS 35 IT yFR ES DE EN INFORMATIONS DE S CURIT IMPORTANTES 36 Les manchons pr sentent un marquage color afin de signaler visuellement la partie rotative Les tiquettes et marquage de s curit doivent tre maintenus propres et lisibles Il convient de les inspecter p riodiquement et de les remplacer si elles ne sont pas lisibles ou perceptibles une distance d observation garantissant la s curit du manipulateur Contactez Miltenyi Biotec pour le remplacement des tiquettes de s curit Consignes de s curit g n rales Si l appareil ne fonctionne pas correctement et ou les instructions ou messages affich s vous avisent de contacter le support technique l utilisation s re de l appareil n est plus longtemps garantie teignez imm diatement l appareil et d branchez le de la prise lectrique puis contactez un prestataire de services Miltenyi Biotec agr ou l quipe de support technique de Miltenyi Biotec
13. donde se utilice el instrumento En la ausencia de una circulaci n adecuada el aire del ambiente puede no enfriar el instrumento a temperaturas de funcionamiento adecuadas Permita una suficiente circulaci n del aire alrededor del instrumento al menos 15 cm en todos los lados durante el manejo para asegurar un enfriamiento adecuado No coloque el instrumento cerca de radiadores registros de calor estufas u otro equipamiento incluyendo amplificadores que genere calor Proteja el instrumento de la luz directa del sol No cubra las ranuras y aperturas del instrumento ya que est n destinadas a la ventilaci n y protecci n contra el sobrecalentamiento del instrumento No coloque el instrumento en un estante empotrado o espacios similarmente confinados a menos que el espacio haya sido espec ficamente designado para proveer una ventilaci n adecuada Siga las instrucciones de montaje del instrumento Aseg rese de que el enchufe principal as como el conector para el cable de energ a est n f cilmente accesibles y colocados tan cerca del operador del instrumento como sea posible Si es necesario desconectar la fuente de energ a desconecte el cable de la toma de corriente El instrumento est equipado con un enchufe el ctrico con tres cables para la toma de tierra que tiene una tercera clavija para la toma Este enchufe solo se adapta a una toma de corriente de tierra Este es un procedimiento de seguridad No intente insertar el enchuf
14. instrument displays the message Memory is worn out File cannot be saved Folder cannot be accessed The instrument displays the message Program folder cannot be opened Table 6 1 Errors sources of errors and user actions Press the tube position on the instrument s display for more information Install heater Reinstall heater Make sure that the heater is installed properly Carefully clean the heater contacts of the instrument and the erps Heater using a cloak soaked with water or 70 ethanol Carefully check the heater for signs of damage If no visible signs of damage can be detected switch the instrument OFF and ON again and retry to run the program Contact Technical Service Delete other program shortcuts in the Favorites folder Restart the device and try again If the problem persists contact Miltenyi Biotec Technical Support 91 92 Color Size Weight Main power Motor Conditions of operation USB interface Length x Width x Height Power consumption Supply voltage Fuse Rotational speed Rotation direction Rotational acceleration Torque Working temperature without use of heaters Working temperature with use of heaters Humidity Altitude Storage temperature For service purposes and for loading and storage of programs Yellow orange 465x285x185 mm 11 kg max 450 W 100 240 VAC 50 60 Hz 2xT3 15AH250V 20 4 000 rpm Clockwi
15. instrumento si esta abierto o desensamblado se ha ca do o ha sido da ado tiene partes da adas o rotas e tiene un cable de energ a da ado un objeto ha entrado a trav s de las ranuras de ventilaci n un objeto extra o ha ca do en el instrumento Si aparecen llamas o humo inmediatamente apague la fuente de energ a desenchufe el instrumento de la toma de corriente y contacte un proveedor de servicios autorizado de Miltenyi Biotec o un servicio t cnico de Miltenyi El instrumento est destinado solo a uso en un lugar cubierto Si alg n fluido entra en el instrumento puede ocasionar cortocircuitos el ctricos shock el ctrico o incendio No permita que fluidos penetren el interior del instrumento Proteja el instrumento contra vertidos y salpicaduras accidentales Limpie inmediatamente los vertidos No utilice el instrumento si se han derramado l quidos en el instrumento No utilice el instrumento en un lugar mojado o h medo Evite reas con alta humedad o vapor Transportar el instrumento de un medio fr o como una habitaci n fr a a 5 C a temperatura ambiente puede causar una condensaci n dentro del instrumento En esos casos espere a que el instrumento se deshumidifique antes de utilizarlo Desenchufe el instrumento de la toma de corriente antes de limpiarlo No utilice agentes limpiadores l quidos o aerosoles use siempre un pa o h medo Aseg rese de que hay suficiente ventilaci n en el lugar
16. of the optical radiation sources Optical radiation of disassembled instruments may be harmful to the eyes Hot surface hazards The heating foil of the gentleMACS Heaters may become hot enough to cause burns if touched Do not touch the heating foil while the instrument is in operation Noise level hazards If the instrument is used at maximum load it might produce noise at a level which could cause a hazard Hazardous noise levels may result in hearing impairment It is recommended that the responsible body measures or calculates the sound pressure level at the operator s position and at whatever point 1 m from the enclosure of the equipment has the highest sound pressure level If it is not ensured during installation that the sound pressure level from the device will not reach a value at its point of use that could cause a hazard then protective materials such as noise reducing baffles or hoods ear protection equipment has to be used Servicing transport and disposal Unless otherwise specifically noted in this user manual or other Miltenyi Biotec documentation do not service the instrument yourself Servicing and repair must be performed by qualified service personnel Improper or incorrect servicing or repair of the instrument can cause hazards to users lead to unpredictable results cause instrument malfunction or damage as well as premature wear and reduced life of the instrument It may also void your warranty When replac
17. or homogenization Multiple samples can be processed with maximum flexibility The gentleMACS Octo Dissociator with Heaters provides for gentle but efficient tissue dissociation for cell separation cell analysis or cell culture as well as thorough tissue homogenization for subsequent subcellular or molecular analyses The instrument is capable of incubating the samples and runs mechanical and enzymatic dissociation procedures in one step Dissociate and discover Figure 1 The gentleMACS Octo Dissociator with Heaters equipped with gentleMACS Tubes and Heaters Table of contents EN DE ES FR IT 2 1 2 2 3 1 3 2 4 2 4 3 4 3 1 Important safety information 07 Wichtige Sicherheitsinformationen 15 Informaci n importante de seguridad 25 Informations de s curit importantes 33 Importanti istruzioni di sicurezza 41 Introduction 49 Preparation of single cell suspensions and homogenates from tissues 49 Sample preparation for cell and molecular biology applications 50 Sample preparation combining enzymatic and mechanical dissociation 51 MACS Tissue Dissociation Kits 51 Pre defined tissue dissociation and homogenization programs 52 Customized dissociation programs 52 Installation 53 Components included in the delivery 53 Installation 54 Materials required 55 Materials required to operate the instrument 55 Spare parts 56 Instrument operation 57 General information for operating the instrument 57 Instrument schematics
18. remplacer la prise femelle L appareil doit uniquement tre utilis partir d une source d nergie qui remplit les sp cifications mentionn es sur l tiquette de puissance lectrique du produit Si vous avez des questions sur le type de source de courant utiliser contactez votre prestataire de services Miltenyi Biotec agr ou votre entreprise locale de production et de distribution d nergie N utilisez aucune rallonge lectrique ou multiprise Ne surchargez pas une prise lectrique 37 IT per ES DE EN INFORMATIONS DE S CURIT IMPORTANTES 38 Dangers m caniques Des pi ces mobiles et tournantes sont de potentiels dangers m caniques Lorsque vous utilisez l appareil veillez ce que la Trough les Sleeves et le Safety Shield y soient bien fix s Quand les rotors de l appareil sont en rotation ne les touchez pas Ne chargez et n enlevez pas les tubes de l appareil Utilisez uniquement des gentleMACS Tubes correctement install s avec des chapes troitement ferm es N utilisez pas l appareil seulement avec des chapes de gentleMACS Tube en place Les tranchants expos s des chapes de gentleMACS Tube peuvent provoquer des incisions si ils sont touch s lorsqu ils sont en rotation Ne contournez aucune mesure ou dispositif de s curit Dangers chimiques et biologiques Selon la mati re biologique utilis e une contamination et infection peut entra ner des blessures corporelles graves ou m me la mort
19. salud humana Por este motivo todos los equipos deben ser espec ficamente recogidos y tratados por los centros de residuos designados y seg n planes de cumplimiento de la WEEE cualificada Al asegurarse de que se est deshaciendo de su equipo el ctrico y electr nico no deseado de acuerdo con la legislaci n y la WEEE aplicable de disposici n de residuos peligrosos estar ayudando a preservar nuestros recursos naturales y a proteger la salud humana Miltenyi Biotec est comprometido con la protecci n del medio ambiente Miltenyi Biotec ofrece productos que se encuentran en el final de su vida a programas de retorno de muchos pa ses y a socios con esquemas de cumplimiento de licencias WEEE en todo el mundo Miltenyi Biotec le ofrece reciclar su equipo de Miltenyi Biotec en el final de su vida til forma gratuita Los t rminos y la disponibilidad de esta oferta pueden variar seg n la regi n geogr fica debido a las diferencias en los requisitos reglamentarios Tenga en cuenta que dependiendo del tipo y uso del equipo pueden aplicarse requisitos adicionales Para m s informaci n o si desea eliminar su equipo de Miltenyi Biotec en el final de su vida til por favor p ngase en contacto con su representante local de Miltenyi Biotec o con el Servicio T cnico de Miltnyi Biotec CAUTION Le gentleMACS Octo Dissociator with Heaters est con u pour une utilisation s re si il est correctement install et mani par un pers
20. un prestataire de services Miltenyi Biotec agr ou l quipe de support technique de Miltenyi Biotec L appareil est uniquement destin une utilisation int rieure Si des fluides p n trent l int rieur de l appareil ceci peut entra ner des courts circuits des chocs lectriques ou des incendies Faites extr mement attention lorsque vous utilisez des liquides Veillez ce qu aucun fluide ne p n tre l int rieur de l appareil Prot gez l appareil contre des d versements et claboussements accidentels Nettoyez imm diatement tout d versement Ne pas utiliser l appareil si des liquides ont coul s dans l appareil Ne pas utiliser l appareil dans des endroits humides et brumeux Evitez des endroits ayant une forte humidit ou condensation Le d placement de l appareil d un environnement froid comme une chambre froide 5 C un endroit temp rature ambiante peut causer une condensation l int rieur de l appareil Dans ces cas l attendez que l appareil soit sec avant de l utiliser D branchez l appareil de la prise lectrique avant de le nettoyer Ne pas utiliser des d tergents liquides ou a rosols utilisez toujours un tissue humide Assurez une circulation ad quate de l air dans la pi ce o l appareil est utilis Si une telle circulation ad quate n est pas assur e l air ambiant ne peut pas refroidir l appareil des temp ratures de fonctionnement acceptable Veillez ce que l air puisse
21. ATION 74 Example of an user defined program 1 ramp 2 000 rpm 10 2 loop 3x 3 spin 2 000 rpm 1 4 spin 2 000 rpm 10 5 end loop 6 temp ON 7 spin 20 rpm 30 8 spin 3 000 rpm 10 9 temp OFF 10 end 4000 3000 2000 1000 rpm o 1000 2000 SON SAT IL a AR a Ara a A AV 1032 Se 79 13 15 07 19 21 231925 27 29 m 337350037 39741043 30min 47 49 51 53 55 time in sec SSS men Figure 4 9 Graph of the example user defined program Note It is possible to insert or edit a command at any step above the end command A new command will be inserted above a highlighted command Commands can be highlighted using the touchscreen or the scroll buttons Note Highlighted commands except for the end command can be deleted by pressing Delete 1 Press spin Enter all parameters for the selected command using the pop up keypad Enter speed rpm and time min and sec Main menu Editor Setup 15 06 ramp loop end loop 2 Press OK to confirm a value or Exit to cancel Parameters for an individual step will be displayed in an abbreviated form In this example spin 2 000 rpm 1 indicates a counter clockwise spin of 2 000 rpm for 1 second new_program 2 end ramp loop end loop temp 3 Press Add to insert the next command or Save to name and store the program for future use refer to step 11 In this example a second spin c
22. C 62471 questo sistema di illuminazione ha un valore di rischio espositivo EHV di 0 91 ed inoltre nel gruppo di rischio estremo Exempt Risk Group La distanza di rischio HD per il Exempt Risk Group di 61 cm La distanza di rischio per il gruppo di rischio 1 di 20 cm Il rischio per gli astanti dipende da come lo strumento installato e dalla modalit di funzionamento La radiazione ottica emessa dai LEDs pu essere nociva per gli occhi a breve distanza se il Trough viene rimosso Si sconnetta lo strumento prima di rimuovere il Trough Una volta rimossa il Trough non si guardi in direzione dell illuminazione dei LEDs se non ad una distanza di almeno 20 cm Anche il lettore di codice 2D integrato usa LEDs per l illuminazione dell area di lettura Non si fissi direttamente nel lettore di codice 2D a breve distanza se questo in funzione Non si smonti si modifichi o si rimuova alcuna delle fonti di radiazione ottica La radiazione ottica degli strumenti smontati potrebbe essere nociva per gli occhi Rischio superficie calda La lamina di riscaldamento del gentleMACS Heaters pu diventare abbastanza calda da causare ustioni se toccata Non toccare la lamina di riscaldamento mentre lo strumento in funzione Rischi di livello acustico Se lo strumento in uso con carico massimo pu generare rumore a livelli che possono essere pericolosi Rumori a livelli pericolosi potrebbero causare danni all udito Si raccomandano
23. MACS Heaters allow to apply 37 C either to all eight positions or individually to each tube By gently pressing the clamps the gentleMACS Heaters can be easily attached to the tube positions thus connecting the gentleMACS Heaters to the instrument via its electrical contact pins Clamps Heating foil Electrical contacts CR _ __o_ oo werdT_ ___ i l e Figure 1 3 The gentleMACS Heaters for incubation of samples at 37 C 1 4 MACS Tissue Dissociation Kits A broad selection of optimized and easy to use MACS Tissue Dissociation Kits including pre defined enzyme mixes allows for convenient and reproducible tissue dissociation using the gentleMACS Octo Dissociator with Heaters A comprehensive list of MACS Tissue Dissociation Kits for numerous applications is available for download at www gentlemacs com si 52 1 5 Pre defined tissue dissociation and homogenization programs More than 40 pre set gentleMACS Programs without heating step and including an incubation step at 37 C have been specifically developed to offer standardized procedures for tissue dissociation in virtually any research field A comprehensive list of optimized gentleMACS Programs and detailed gentleMACS Protocols for numerous applications are available for download at www gentlemacs com 1 6 Customized dissociation programs In addition to the ready to use gentleMACS Programs optimized for specific applications the ge
24. S Programs in the respective folder will be displayed Note Programs can be selected from a total of eight folders Favorites Miltenyi Templates and five User folders Press the folder icon several times to scroll through 3 Highlight the desired gentleMACS Program touching the screen or using the scroll buttons Note Press the column header Programs to sort programs within a folder alphabetically Press the column header Date to sort programs by the date of creation 4 Select the required program and press OK to confirm The program will be assigned to all eight tube positions The status of the tube positions will change to Ready Programs Tube Date y 37C_m_tumor_01 C 19 Apr 2013 67 BE INSTRUMENT OPERATION Note Use the Clear button to reset positions from the Ready status into the Selected status to assign new programs 5 Press Start to commence the program The status of the tube positions will change to Running The progress bar and timer indicate the time remaining Note After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start The program will immediately start and the status of the tube positions will change to Running Programs Tube Date y 37C_m_tumor_01 C 19 Apr 2013 6 Oncompletion of the program the status of the tube positions will change to Done The progress bar and timer display the elapsed time since completion of the program Programs Tube Dat
25. The following example shows the change of date 1 Press Set date 2 Press the desired parameter day dd month mm and year yyyy 3 Modify the parameter using the touchscreen 4 Press OK to confirm or Exit to cancel 4 6 3 Set folder name To name or rename User folders 1 Press Set folder name 2 Select the required folder Note Default values for User folders are User _1 User_2 User 3 User 4 User 5 3 Enter the new name using the touchscreen The folder name cannot exceed nine characters 4 Press OK to save or Exit to cancel 83 BE INSTRUMENT OPERATION 84 4 6 4 Software version To display the software version Press Software version The software versions and gentleMACS Program database version will be displayed 4 6 5 Serial number To display the serial number of the instrument Press Serial number The serial number will be displayed L WARNING A WARNING If the gentleMACS Octo Dissociator with Heaters needs servicing send the instrument back to an authorized Miltenyi Biotec service provider Ensure that all parts are decontaminated and sterilized for details refer to section 5 2 Use the original packaging material Do not service the instrument yourself Service needed on components inside the instrument must be carried out only by personnel trained and authorized by Miltenyi Biotec Servicing and an improperly serviced instrument may result in mechanical haz
26. Tout pr l vement clinique doit tre consid r comme potentiellement contagieux Si des mati res biologiquement dangereuses sont ou ont t utilis es l op rateur doit choisir et porter un quipement et des v tements de protection tel indiqu dans les avertissements et mesures de s curit pour la substance respective La mesure de s curit mentionn e ci dessus s applique galement pour toute substance chimique dangereuse y compris toxique ou corrosive acide ou radioactive pouvant tre pr sente dans le pr l vement Portez des gants et des v tements de protection ainsi que des lunettes protectrices pour que la substance n entre pas en contact avec le visage et les yeux Un quipement de protection d fectueux ou inad quate est dangereux L appareil doit tre utilis dans une hotte de s curit si des mati res dangereuses ou inconnues sont trait es Si des mati res dangereuses ont t utilis es ou renvers es l appareil doit soigneusement et compl tement tre d contamin Il est strictement interdit de continuer l utilisation d accessoires ou de pi ces de l appareil contamin es Des tubes et tout autre consommable qui taient en contact avec des mati res biologiquement dangereuses doivent tre d contamin s avant d tre jet s Tout liquide et d chet solide doivent tre consid r s comme dangereux et pour cela tre trait s selon les pr cautions universelles de laboratoire l enl vement de d che
27. ali come indicato negli avvisi e le precauzioni specifiche per la particolare sostanza Le precauzioni di sicurezza sopra indicate considerano qualsiasi altra sostanza chimica rischiosa incluse sostanze tossiche e corrosive acide o radioattive che potrebbero essere presenti nel campione Si indossino guanti e abiti di sicurezza nonch occhiali protettivi per prevenire il contatto tra la sostanza ed il corpo e gli occhi L uso di abbigliamento protettivo danneggiato o inadeguatoo pu essere pericoloso Lo strumento deve essere impiegato all interno di un area protetta nel caso vengano impiegati materiali pericolosi o sconosciuti Nel caso in cui del materiale percoloso sia stato utilizzato o sia fuoriuscito si faccia molta attenzione quando si sterilizzi decontamini lo strumento E severamente proibito continuare ad utilizzare parti contaminate o altri accessori dello strumento Le provette o il materiale ausiliario entrati in contatto con materiali biologici pericolosi dovranno essere sterilizzati prima dello smaltimento Tutt i rifiuti liquidi e solidi dovranno essere considerati pericolosi e quindi essere trattati secondo le precauzioni universali da laboratorio Lo smaltimento dei rifiuti dovr avvenire in accordo con le normative locali vigenti Rischi di radiazioni ottiche Lo strumento fornito di un potente sistema di diodi luminosi LEDs per illuminare il Safety Shield ed i gentleMACS Tubes Secondo gli standard internazionali IE
28. ame and duration are displayed In this example program m_neoheart_01_01 will be completed within 1 minute The program m_ neoheart_01_01 is running The progress bar and timer display the time remaining to complete the program in this example 15 seconds The program m_ neoheart_01_01 is finished The timer displays the elapsed time since completion of the program in this example 3 seconds The program was stopped It is possible to abort any program by pressing the required tube position on the touchscreen The timer displays the remaining time to complete the program since abortion in this example 58 seconds The tube position was deselected Inserted tubes are automatically assigned the status Selected Deselection of an inserted tube is achieved by pressing the required tube position on the touchscreen The status of the tube position will change to Stand by Note that programs cannot be assigned to tube positions in the Stand by mode Table 4 4 Overview of tube position statuses 63 64 44 2 Processing samples Tips and hints for the processing of samples The tubes are for single use only single procedure Only use C Tubes with the gentleMACS Heaters Sample sizes range from 300 uL to maximal 10 mL total volume and 20 to 4 000 mg of tissue The maximal amount of tissue depends on the consistency of the tissue as well as on the respective gentleMACS Protocol Dep
29. aminierung oder zum Versand des Ger tes haben wenden Sie sich zur Unterst tzung bitte direkt an unseren Miltenyi Biotec Technical Support Kundeninformation zur Entsorgung von Elektro und Elektronik Altger ten Waste of Electrical and Electronic Equipment WEEE Bitte entsorgen Sie Ihre Altger te von Miltenyi Biotec unter Einhaltung der jeweils geltenden Vorschriften f r die Erfassung und Behandlung von Elektro und Elektronik Altger ten und die Entsorgung von Gefahrstoffen Diese k nnen von Land zu Land sowie regional variieren Elektrische und elektronische Ger te k nnen Gefahrstoffe enthalten welche die Umwelt erheblich belasten und oder die Gesundheit gef hrden Deshalb m ssen Altger te speziellgesammelt und durch ausgewiesene Entsorgungsbetriebe im Rahmen der hierf r vorgesehenen Entsorgungssysteme fachgerecht behandelt werden Indem Sie sicherstellen dass Ihr Altger t gem den geltenden Vorschriften zur Behandlung von Elektro und Elektronik Altger ten sowie von Gefahrstoffen entsorgt wird tragen Sie dazu bei unsere nat rlichen Ressourcen zu schonen und die menschliche Gesundheit zu sch tzen Miltenyi Biotec setzt sich f r den Schutz der Umwelt ein Miltenyi Biotec bietet in zahlreichen L ndern eigene R cknahmeprogramme f r Altger te an und arbeitet weltweit mit lizensierten Partnern zusammen die an bestehende Recycling und Entsorgungssysteme angeschlossen sind Miltenyi Biotec erm glicht Ihnen ein kostenlos
30. arch use only and not for therapeutic or diagnostic use gentleMACS Octo Dissociator with Heaters User manual Version 1 EN Original instructions Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 0 Fax 49 2204 85197 macs miltenyibiotec de www miltenyibiotec com Read the user manual before using the instrument Before using the instrument read chapter Important safety information and all other information contained in this user manual including all safety and operating instructions Pay special attention to warnings displayed on the instrument Failure to read and follow these guidelines could lead to improper or incorrect usage and result in damage to the instrument Improper usage could also cause severe personal injury death unpredictable results instrument malfunction and premature wear of components shortening the lifetime of the instrument Such actions may void your warranty Keep this user manual and any other safety and operating instructions provided with the instrument in a safe place that is accessible to all users for reference If you are concerned about the safe use of the instrument please contact your authorized Miltenyi Biotec service provider or call Miltenyi Biotec Technical Support Thank you for choosing a Miltenyi Biotec product The gentleMACS Octo Dissociator with Heaters is an innovative benchtop instrument for automated tissue dissociation
31. ards optical radiation hazards electric shock or personal injury and even death Read the chapter Important safety information Read the section Risk of electric shock in the chapter Important safety information Unplug the power cord before cleaning the instrument Do not allow fluids or objects to enter the interior of the instrument Large amounts of accidentally spilled liquid are automatically collected in the Trough of the gentleMACS Octo Dissociator with Heaters Use a pipette to aspirate the liquid before removing the Trough for cleaning and sterilizing If biohazardous material is or has been used always use personal safety equipment while removing parts of the gentleMACS Octo Dissociator with Heaters for cleaning or sterilization In case of spillage or contamination clean the instrument appropriately For decontamination wipe the instrument with 70 ethanol Clean the instrument only with cleaning agents that are non corrosive and not damaging to the plastic housing of the gentleMACS Octo Dissociator with Heaters Suitable cleaning agents include household detergents and 70 ethanol Do not use acetone or any aggressive agents If in doubt contact Miltenyi Biotec Technical Support Note Do not leave gentleMACS Heaters and Tubes attached to the instrument while it is not in use 85 86 BR W N Unplug the power cord before cleaning the instrument Remove the Safety Shield Remove the Sleeves from the instrument for cleanin
32. ate heat Protect the instrument from direct sunlight Do not cover the slots and openings of the instrument as these are provided for ventilation and protect the instrument from overheating Do not place the instrument in a built in rack or similar confined spaces unless the space has been specifically designed to provide proper ventilation Follow the mounting instructions for the instrument Ensure that the mains switch as well as the connector for the power cable are easily accessible and located as close to the operator of the instrument as possible If it is necessary to disconnect the power supply unplug the cable from the power outlet The instrument is equipped with a three wire electrical grounding type plug that has a third pin for grounding This plug only fits into a grounded power outlet This is a safety feature Do not try to insert the plug into a non grounded power outlet If you cannot insert the plug into the outlet contact your local electrician to replace the outlet The instrument should only be operated from a power source that meets the specifications mentioned on the product s electrical ratings label If you have questions about the type of power source to use contact your authorized Miltenyi Biotec service provider or local power company Do not use extension cords or power strips Do not overload an electrical outlet Mechanical hazards Moving and revolving parts are potential mechanical hazards When operating th
33. bien circul au tour de l appareil pendant son fonctionnement au moins 15 cm des deux c t s pour garantir un refroidissement ad quat vitez de placer l appareil pr s de radiateurs d accumulateurs de chaleur de po les ou d autres dispositifs y compris des amplificateurs g n rant de la chaleur Ne pas exposer l appareil au soleil Ne couvrez pas les fentes et les lumi res de l appareil car elles servent de ventilation et vite une surchauffe de l appareil Ne placez pas l appareil dans un rack encastr ou des espaces confin s similaires moins que cet espace ait sp cialement t con u pour fournir une ventilation convenable Suivez les instructions de montage de cet appareil Assurez que l interrupteur g n ral ainsi que le connecteur pour le c ble lectrique soient facilement accessibles et qu ils se trouvent le plus proche possible de l op rateur de l appareil S il est n cessaire de couper l alimentation lectrique d branchez le c ble de la prise lectrique L appareil est quip d une prise lectrique trois fils et mise la terre avec une troisi me broche de connecteur pour la mise la terre Cette prise ne s embo te que dans une prise de courant mise la terre ceci pour des raisons de s curit N essayez pas d introduire la prise dans une prise femelle de courant non mise la terre Si vous ne parvenez pas introduire la prise dans une prise femelle contactez votre lectricien local pour
34. c non offre alcuna garanzia n accetta alcuna responsabilit per danni alla strumentazione o guasti dovuti ad un uso sconsiderato del componenti Dopo aver completato ogni servizio di manutenzione e lavoro di riparazione ci si assicuri che il 47 m FR ES DE EN IMPORTANTI ISTRUZIONI DI SICUREZZA 48 service provider Miltenyi Biotec autorizzato esegua tutti i controlli di sicurezza previsti dalle procedure per garantire che lo strumento funzioni in modo corretto Si usino solo opzioni ed aggiornamenti raccomandati da Miltenyi Biotec Si chiedano informazioni al rappresentante locale di Miltenyi Biotec in merito al servizio esteso di strumenti Miltenyi Biotec s e le modalit di supporto o ci si riferisca a www miltenyibiotec com support Lo strumento dovrebbe essere trasportato con cura nell imballo specificato da Miltenyi Biotec Danni interni possono essere provocati da eccessive vibrazioni o se lo strumento dovesse cadere Nel caso l apparecchio debba essere spedito al produttore lo si sterilizzi per eliminare ogni sostanza pericolosa prima della spedizione Nel caso ci fossero domande in merito all adeguata pulizia o la spedizione si contatti per l assisenza il Miltenyi Biotec Technical Support Informazioni per i clienti riguardo lo smaltimento delle apparecchiature elettriche ed elettroniche secondo le normative WEEE Waste of Electrical and Electronic Equipment Si prega di smaltire i prodotti Miltenyi Biotec in disu
35. cient air circulation To operate the instrument the display and the top of the instrument must be accessible To install the gentleMACS Octo Dissociator with Heaters 1 Carefully remove the device from the cardboard box 2 Place the instrument on a secure and stable workbench that can support its weight and size Ensure that the instrument is not exposed to direct sunlight CAUTION Do not place the instrument on an unstable table cart stand tripod or bracket As a consequence the instrument might fall down This may cause serious bodily harm and or serious damage to the instrument Use only on a table cart stand tripod or bracket recommended by Miltenyi Biotec Plug the instrument into the power mains WARNING The instrument is equipped with a three wire electrical grounding type plug This is a safety feature to reduce the risk of electric shock Do not insert the plug into a non grounded power outlet If you cannot insert the plug into the outlet or if you are not sure whether the outlet is grounded contact your local electrician The instrument should only be operated from a power source whose specifications match those indicated on the instrument electrical ratings label If you have questions about the type of power source to use contact your authorized Miltenyi Biotec service provider or local power company Ensure that the mains switch as well as the connector for the power cable are easily accessible and loca
36. d in before setting the switch to the ON position indicates ON O indicates OFF 2 The instrument starts within a few seconds and displays the Main menu Note Do not switch off the instrument while running a program to avoid severe damage of the instrument The gentleMACS Octo Dissociator with Heaters is operated using the touchscreen display Items that can be selected are displayed in yellow while items that cannot be selected are displayed in white Buttons and symbols Buttons Icon Function Description Al scroll up The scroll buttons are used to scroll up or down e g to highlight a gentleMACS v scroll down Program in the Main menu tab or device settings in the Setup tab Mm Info button A pop up screen is displayed when pressing on the Info button and displays information about the highlighted gentleMACS Program including program name selected tube type date of program creation duration ofthe program rounds per run rpr and temperature C applied Table 4 1 Buttons of the gentleMACS Octo Dissociator with Heaters display 59 BE INSTRUMENT OPERATION Folders Different folders can be accessed by pressing a folder icon The arrow indicates scroll function Miltenyi The Miltenyi folder contains gentleMACS Programs Miltenyi provided by Miltenyi Biotec The lock on the Miltenyi folder indicates that programs in this folder cannot be edited or deleted Templates The Templates
37. e 37C_m_tumor_01 C 19 Apr 2013 Note For further processing use the Clear button to reset all positions from the Done status into the Selected status to assign new programs 7 Remove the tubes from the tube positions The status of the tube positions will change to Free 4 4 2 3 Running multiple samples sequentially Multiple samples can be run sequentially In this example three samples are processed using three different programs Sample A is processed with program m_brain_01_01 a Miltenyi Biotec program in the Miltenyi folder optimized for the preparation of single cell suspensions from mouse neural tissue Sample B is processed with program 37 C_m_tumor_01 a Miltenyi Biotec program in the Miltenyi folder optimized for the preparation of single cell suspensions from mouse tumor including an incubation step Sample Cis processed with program C_01 a template program provided by Miltenyi Biotec in the Templates folder Programs in the Templates folder can also be used as a basis to develop customized programs Note Do not switch off the instrument while running a program to avoid severe damage of the instrument 1 Insert Sample A into a free position The status of the tube position will change from Free to Selected 2 Press the Folder icon to locate the Miltenyi folder Programs Tube Date m_brain_02_01 C 01 Mar 2011 0 C 01 Mar 2011 3 Select the program m_brain_01_01 from the list and press OK to conf
38. e instrument keep the Trough Sleeves and Safety Shield attached to it When the instrument s rotors are rotating do not touch them or load or remove tubes from the instrument Only use properly installed gentleMACS Tubes with tightly closed caps Do not operate the instrument with only gentleMACS Tube caps in place The exposed cutting edges of the gentleMACS Tube caps may lead to incision wounds if touched while they are rotating Do not circumvent any safety measures or devices IT FR ES DE Pen IMPORTANT SAFETY INFORMATION 12 Chemical and biological hazards Depending on the biological material used contamination or infection may lead to severe personal injury or death All clinical samples must be considered potentially infectious If biohazardous material is or has been used the operator must choose and wear personal safety equipment as indicated in the warnings and precautions for the particular substance The above safety precaution also accounts for any hazardous chemical including toxic or corrosive chemicals acidic or radioactive substances that may be present in the sample Wear protective gloves and clothing as well as safety glasses to prevent contact of the substance with skin and eyes Defective or inadequate safety equipment is hazardous The instrument must be operated in a safety hood if hazardous or unknown materials are being processed If hazardous material has been used or spilled care must be taken to
39. e Sweden Phone 0200 111 800 Fax 046 280 72 99 Customer service Denmark Phone 80 20 30 10 Fax 46 46 280 72 99 Customer service Norway Finland Iceland and Baltic countries Phone 46 46 280 72 80 Fax 46 46 280 72 99 Singapore Miltenyi Biotec Asia Pacific Pte Ltd 100 Beach Road 28 06 to 28 08 Shaw Tower Singapore 189702 Phone 65 6238 8183 Fax 65 6238 0302 macs miltenyibiotec com sg South Korea Miltenyi Biotec Korea Petra building 659 Yeoksam dong Gangnam gu Seoul South Korea Phone 82 2 555 1988 Fax 82 2 555 8890 macs miltenyibiotec co kr Spain Miltenyi Biotec S L C Luis Bu uel 2 Ciudad de la Imagen 28223 Pozuelo de Alarc n Madrid Spain Phone 34 91 512 12 90 Fax 34 91 512 12 91 macs miltenyibiotec es United Kingdom Miltenyi Biotec Ltd Almac House Church Lane Bisley Surrey GU24 9DR UK Phone 44 1483 799 800 Fax 44 1483 799 811 macs miltenyibiotec co uk www miltenyibiotec com Miltenyi Biotec provides products and services worldwide Visit www miltenyibiotec com local to find your nearest Miltenyi Biotec contact Unless otherwise specifically indicated Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use gentleMACS and MACS are registered trademarks or trademarks of Miltenyi Biotec GmbH All other trademarks mentioned in this publication are the property of their respective owners and are used for identification purposes on
40. e attorno allo strumento almeno 15 cm su tutti i lati durante il funzionamento per garantire un adeguato raffreddamento Non si posizioni lo strumento vicino a radiatori fonti di calore fornelli o qualsiasi altro apparecchio che generi calore inclusi gli amplificatori Non si coprano gli slots e le aperture dello strumento poich le stesse sono previste per la ventilazione e per proteggere lo strumento da surriscaldamento Non si posizioni lo strumento in uno scomparto integrato o in un analogo spazio limitato a meno che esso non si stato appositamente realizzato in modo tale da garantire un appropriata ventilazione Si seguano le istruzioni di montaggio dello strumento Ci si assicuri che gli interruttori di rete principali cos come il cavo di connessione alla corrente siano facilmente accessibili e ubicati il pi vicino possibile all operatore Se necessario sconnettaere l alimentazione si scolleghi il cavo dalla presa di corrente Lo strumento fornito di una spina con tre conduttori ed un terzo pin per il collegamento a terra Questa spina pu essere collegata solamente con una presa di corrente a terra Si tratta di una misura di sicurezza Non si provi ad inserire la spina in una presa non a terra Nel caso non si riesca ad inserire la spina nella presa si contatti l elettricista di fiducia per sostituirla Lo strumento dovrebbe venire alimentato solamente da una fonte di alimentazione che rispetti le specificazioni me
41. e en una toma de corriente que no sea de tierra Si no puede insertar el enchufe en la toma contacte a su electricista local para reemplazar la toma El instrumento deber a ser utilizado desde una fuente de energ a que cumple las especificaciones mencionadas en la etiqueta de potencia el ctrica del producto Si tiene preguntas sobre el tipo de fuente de energ a que usar contacte a su proveedor de servicios autorizado de Miltenyi Biotec o una compa a de energ a local No use alargadores o enchufes m ltiples No sobrecargue una toma de corriente Riesgos mec nicos Mover y quitar piezas son potenciales riesgos mec nicos Cuando utilice el instrumento mantenga la Trough Sleeves y Safety Shield junto a l No toque o quite los tubos del instrumento cuando los rotores del instrumento est n rotando Utilice solo gentleMACS Tubes instalados adecuadamente con tapas fuertemente 29 IT FR Es DE EN INFORMACI N IMPORTANTE DE SEGURIDAD 30 cerradas No utilice el instrumento con solo las tapas de los gentleMACS Tubes en su lugar Los bordes cortantes de las tapas de los gentleMACS Tubes pueden llevar a heridas por corte si se tocan mientras est n rotando No ignore ninguna medida o aparato de seguridad Riesgos qu micos y biol gicos Dependiendo del material biol gico utilizado una contaminaci n o infecci n puede llevar a graves da os personales o muerte Todas las muestras cl nicas deben ser consideradas potenc
42. e mati re dangereuse avant le chargement Pour toute question concernant une d contamination ou un chargement correct veuillez contacter le support technique de Miltenyi Biotec D chets d Equipements Electriques et Electroniques DEEE Information Client Pensez recycler vos produits Miltenyi Biotec en fin de vie en conformit avec la directive DEEE en vigueur dans votre pays Les quipements lectriques et lectroniques peuvent contenir des substances dangereuses qui peuvent avoir un effet n faste sur l environnement et ou la sant humaine C est pourquoi tous les quipements doivent tre sp cifiquement collect s et trait s par les centres d sign s et ce en conformit avec la R glementation DEEE En vous assurant que vous liminez vos quipements lectriques et lectroniques en accord avec la l gislation en vigueur vous contribuez pr server nos ressources naturelles et prot ger la sant humaine La protection de l environnement est au c ur de nos pr occupations Ainsi nous finan ons de nombreux programmes de collecte et de recyclage des quipements lectriques que nous mettons sur le march en reversant une partie du prix de vente de nos produits des amp co organismes agr s travers le monde Miltenyi Biotec vous permet de recycler gratuitement vos quipements Miltenyi Biotec en fin de vie Les conditions et la disponibilit de cette offre varient selon les pays et les diff rentes e
43. ement or spare parts are required ensure that the service provider uses only genuine Miltenyi Biotec parts or third party parts specified and recommended by Miltenyi Biotec Using unauthorized parts can cause malfunction of the instrument and impair results Miltenyi Biotec does not honor any warranty or accept any responsibility for instrument failure or damages resulting from the use of inappropriate parts After completing any service or repair work ensure that your authorized Miltenyi Biotec service provider performs all safety checks as required by the repair procedure to ensure that the instrument is operating correctly Only use options and upgrades recommended by Miltenyi Biotec Inquire with your local Miltenyi Biotec representative about Miltenyi Biotec s extensive instrument service and support arrangements or refer to www miltenyibiotec com support The instrument should be transported with care in packaging specified by Miltenyi Biotec Internal damage can occur if the instrument is subjected to excessive IT FR ES DE Pen IMPORTANT SAFETY INFORMATION 14 Vibration or dropped If the instrument needs to be shipped back to the manufacturer for service decontaminate the instrument to remove any hazardous material prior to shipment If you have questions regarding proper decontamination or shipment contact Miltenyi Biotec Technical Support for assistance Waste of Electrical and Electronic Equipment WEEE customer info
44. endent on the consistency of the tissue it might be recommended to cut the sample into smaller pieces before processing on the gentleMACS Octo Dissociator with Heaters The tubes are suitable for a broad range of tissues for example spleen liver or brain However very hard material such as bone may damage the tubes For further details refer to the respective gentleMACS Tube data sheets and to the gentleMACS Protocols at www gentlemacs com The following instructions assume that the instrument was successfully installed and initiated as outlined in sections 2 2 and 4 3 To process samples transfer an appropriate amount of tissue and buffer or other appropriate fluid into a gentle MACS C Tube or M Tube Note Tightly close the tube beyond the first resistance 4 4 2 1 Running a single sample Note Do not switch off the instrument while running a program to avoid severe damage of the instrument 1 Available tube positions are indicated as Free Programs Tube Date y 37C_m_tumor_01 C 19 Apr 2013 2 3 Attach the tube upside down to the Sleeve of an available tube position The instrument will automatically detect the inserted tube and assign the tube status Selected Programs Tube Date y 37C_m_tumor_01 C 19 Apr 2013 Note Please make sure that the tube is inserted and removed in an upright position without tilting Tilting the tube at an angle might result in damage of the tube seal and subsequent leakage No
45. entation of the Trough and the Safety Shield is correct 5 3 Instrument disposal The gentleMACS Octo Dissociator with Heaters must be disposed of in accordance with the biosafety level of its use Potentially contaminated parts must be sterilized before disposal in accordance with local laboratory regulations WARNING Decontamination is the responsibility of the operator of the instrument Contaminated instruments or contaminated parts of the instrument must not be sent or given to any person 87 Ea MAINTENANCE 88 Liquid droplets appear in the Trough Tube is blocked during a run Program is automatically aborted and the instrument displays the message Motor is blocked Program was aborted Tube comes offthe instrument Tube is not closed properly Sample is jammed between the cap and the tube and damages the seal This might cause liquid to run through the seal Tube seal is leaky Sleeve is worn Inappropriate sample material If the motor is blocked due to a high resistance of the sample material the instrument will try three times to get started again If the blockage can be resolved the instrument will continue the run of the program If the blockage cannot be resolved the program will automatically be aborted Tube is not correctly fitted into the snap fit of the Trough Tube is not closed properly Close the tube tightly beyond the first resistance The top area
46. entes Contacte la autoridad local a cargo de la fuente de energ a el ctrica construcciones mantenimiento o seguridad para m s informaci n sobre la instalaci n segura y manejo del instrumento Se usan se ales para identificar mensajes de seguridad y de da o de la propiedad Las siguientes se ales se usan a trav s de este manual de usuario o WARNING indica una situaci n potencialmente peligrosa la cual si no se evita puede resultar en muerte o da os graves o CAUTION indica una situaci n potencialmente peligrosa la cual si no se evita puede resultar en un da o menor o moderado Tambi n puede ser usado para alertar contra pr cticas peligrosas 25 26 ePpbbb gt b P QO o Simbolos de seguridad Los siguientes simbolos se usan para destacar condiciones que podrian causar da os al personal o da os al equipo Alerta de seguridad Riesgo de peligro Es necesario consultar la documentaci n del equipo siempre que este s mbolo de alerta de seguridad aparezca para de esta manera poder averiguar la naturaleza del peligro potencial existente y las acciones necesarias a realizar Riesgo biol gico Riesgo de contaminaci n si se usa material biol gico potencialmente peligroso Riesgo de shock el ctrico Radiaci n ptica peligrosa Riesgo involuntario de captura de dedo por parte rotatoria peligrosa Peligro superficie caliente Bloques de conexi n para conductores de protecci n El s mbolo est pegad
47. ento Segnali di avviso sono in uso per identificare un uso sicuro e messaggi in caso di danni a cose personali seguenti segnali saranno in uso in questo manuale o WARNING indica una situazione potenzialmente pericolosa che se non evitata potrebbe causare morte o gravi danni o CAUTION indica una situazione potenzialmente pericolosa che se non evitata potrebbe causare danni minori e lesioni moderate Puo essere impiegato per segnalare procedure pericolose 41 42 EEE gt O 9 Simboli di sicurezza seguenti simboli sono impiegati per sottolineare condizioni che potrebbero causare lesioni a persone o danni all attrezzatura Avviso di sicurezza Rischio di pericolo Consultare il manuale di istruzioni ogni volta che questo simbolo di avvertimento viene utilizzato sul dispositivo per identificare la natura del potenziale pericolo e stabilire quali azioni intraprendere Rischio biologico Rischio di contaminazione se in uso materiale biologio potenzialmente pericoloso Rischio di scosse elettriche Radiazione ottica pericolosa Rischio di intrappolamento involontario delle dita a causa di parti rotanti Superficie calda pericolosa Morsetto di protezione Questo simbolo applicato internamente allo strumento Si tratta di informazioni per il personale di servizio ON Alimentazione OFF Alimentazione Tutte le istruzioni operative devono essere lette prima di installare ed impiegare lo s
48. erador y en cualquier punto alejado 1 metro del equipo donde tiene su nivel de presi n m s alto Si no se asegura durante la instalaci n que el nivel de presi n del sonido del aparato no alcanzar en este punto de uso un valor que podr a suponer un riesgo entonces debe usarse equipamiento de protecci n como cubiertas reductoras del ruido Servicio transporte y eliminaci n seguros A menos que de otro modo se alado en este manual de usuario o en otra documentaci n de Miltenyi Biotec no repare o revise el instrumento Usted mismo Las revisiones y reparaciones deben ser llevadas a cabo por personal de servicio cualificado Una reparaci n o revisi n inadecuada o incorrecta del instrumento puede causar riesgos a los usuarios llevar a resultados impredecibles causar el mal funcionamiento o da o del instrumento as como un prematuro desgaste y vida reducida del instrumento Tambi n puede invalidar su garant a Cuando sean requeridas piezas de repuesto aseg rese de que el proveedor de servicios usa solo piezas originales de Miltenyi Biotec o piezas de terceros especificadas y recomendadas por Miltenyi Biotec El uso de piezas no autorizadas puede causar un mal funcionamiento del instrumento y resultados da ados Miltenyi Biotec no se hace responsable de ninguna garant a o de un error o da o del instrumento resultante del uso de piezas inadecuadas Al finalizar cualquier servicio o reparaci n aseg rese de que su proveedor de ser
49. erdo con el est ndar internacional IEC 62471 este sistema de luces tiene un valor de riesgo por exposici n EHV de 0 91 y es superior al Grupo Exento de Riesgo La distancia de riesgo HD para el Grupo Exento de Riesgo es 61 cm La distancia de riesgo para el Grupo de Riesgo 1 es 20 cm El riesgo del visor depende de c mo se instala y utiliza el instrumento La radiaci n ptica emitida por estos LEDs puede ser da ina para los ojos a distancias cortas si se quita la Trough Desenchufe el instrumento antes de quitar la Trough Si se quita la Trough no mire fijamente la iluminaci n de los diodos LEDs en funcionamiento desde una distancia menor a 20 cm Adem s el lector de c digos 2D integrado utiliza LEDs para iluminar el rea de lectura No mire directamente al lector de c digos 2D desde una distancia corta mientras est en funcionamiento No desensamble modifique o elimine ninguna de las fuentes de radiaci n ptica La radiaci n ptica de instrumentos desensamblados puede ser perjudicial para los ojos Riesgos superficie caliente La l mina calefactada de los calenfactores gentleMACS puede llegar a causar quemaduras si se toca No tocar la l mina calefactada mientras el equipo est en funcionamiento Riesgos del nivel de ruido Si el instrumento se usa en su carga m xima puede producir problemas de o do Se recomienda que el organismo responsable mida o calcule el nivel de presi n del sonido en la posici n del op
50. es Recycling Ihres Altger tes Die Bedingungen und die Verf gbarkeit dieses Angebots unterscheiden sich geographisch aufgrund unterschiedlicher regulatorischer Anforderungen Bitte beachten Sie dass je nach Art und Nutzung Ihres Ger tes zus tzliche Anforderungen gelten k nnen F r weitere Informationen oder wenn Sie Ihr Miltenyi Biotec Altger t entsorgen m chten wenden Sie sich bitte an Ihren lokalen Miltenyi Biotec Vertreter oder den Miltenyi Biotec Technical Support 23 IT FR ES oe EN WICHTIGE SICHERHEITSINFORMATIONEN 24 A VARNING CAUTION EI gentleMACS Octo Dissociator with Heaters est dise ado para un uso seguro si se instala correctamente y se maneja por personal cualificado de acuerdo con las pr cticas generales de seguridad y las instrucciones de este manual de usuario Las directrices en esta secci n explican los riesgos potenciales asociados con el manejo de este instrumento y proporcionan una importante informaci n complementaria para minimizar los riesgos Siga las instrucciones atentamente para protegerse a s mismo a otros y al equipamiento de riesgos potenciales y crear un ambiente de trabajo seguro Use este instrumento solo como especificado por el fabricante para evitar da os en el equipo y lesiones en el personal Siga siempre las instrucciones de seguridad y pol tica de laboratorio del rea de trabajo local as como los est ndares de salud seguridad y prevenci n de accid
51. es zu Kurzschluss Stromschlag oder Feuer kommen Seien Sie vorsichtig im Umgang mit Fl ssigkeiten Verhindern Sie dass Fl ssigkeit in das Innere des Ger tes gelangt Sch tzen Sie das Ger t vor versch tteten oder verspritzten Fl ssigkeiten Beseitigen Sie ausgetretene oder versch ttete Fl ssigkeit sofort Benutzen Sie das Ger t nicht sollte Fl ssigkeit in das Innere des Ger tes gelangt sein Setzen Sie das Ger t nicht in einer nassen oder feuchten Umgebung ein Vermeiden Sie Arbeitsbereiche mit hoher Luftfeuchtigkeit oder Kondensation Das berf hren des Ger tes von einer kalten Umgebung z B aus einem K hlraum bei 5 C in einen Raum mit Raumtemperatur kann zu Kondensation im Inneren des Ger tes f hren Warten Sie in solchen F llen mit der Benutzung des Ger tes bis dieses getrocknet ist Ziehen Sie den Netzstecker vor der Reinigung des Ger tes Verwenden Sie keine Fl ssig oder Spr hreinigungsmittel sondern nur ein feuchtes Tuch Erm glichen Sie im Betrieb eine ausreichende Luftzirkulation Die das Ger t umgebende Luft reicht nicht aus das Ger t bei unzureichender Bel ftung auf einer sicheren Betriebstemperatur zu halten Erm glichen Sie im Betrieb eine ausreichende Luftzirkulation einen Abstand von mindestens 15 cm in allen Richtungen um das Ger t um eine ausreichende K hlung zu gew hrleisten Das Ger t darf nicht in der N he von Radiatoren Hei l ftern fen oder anderen W rme erzeugenden Ger ten a
52. esigned to provide reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna Increase the distance between the equipment and the receiver Connect the equipment to an outlet on a circuit different from that to which the receiver is connected Consult the dealer or an experienced radio TV technician for help Changes or modifications of the instrument unless expressly approved by Miltenyi Biotec may void your authority to operate the instrument pursuant to FCC 47 CFR The gentleMACS Octo Dissociator with Heaters complies with all essential requirements of the following directives 2004 108 EC electromagnetic compatibility 2006 42 EC machinery and is in conformity with the following harmonized standards EN 61010 1 EN 61010 2 010 EN 61010 2 051 EN 61326 1 EN 62471 CE For other safety considerations refer to the product labe
53. folder contains gentleMACS Programs provided by Miltenyi Biotec Programs in this folder can be edited but must be renamed and saved in a User folder E T E E n Hi FE User 1 Five User folders are available to manage user defined programs a y 4 User 2 Dry User 3 SU User 4 sr User 5 pro Favorites Links of programs in the Favorites folder enable Favorites quick access USB stick A USB stick can be installed to transfer programs to SE stick and from a folder Table 4 2 Overview of folders and respective icons LEDs Various statuses of the instrument are indicated by LEDs illuminating the Safety Shield and the gentleMACS Tubes Green Steady Status of one or more positions is Free Note that Editor and Setup menus are illuminated by a steady green light Blue Steady Status of all eight positions is Running Blue Flashing Status of one or more positions is Done Red Flashing Error occurred on one or more positions Table 4 3 Overview of LED colors modes and status 4 3 1 Menu tabs The gentleMACS Octo Dissociator with Heaters is controlled using three menus present on the touchscreen of the instrument Main menu The Main menu displays the availability and status of all eight gentle MACS Tube positions Each tube position is diagrammatically shown as a square panel at the top of the screen In this user manual each panel is referred to as a tube position _Editor_
54. folder contains gentleMACS Programs From Miltenyi to that have been developed by Miltenyi Favorites folder Biotec for a variety of applications User folder There are a total of five user folders From any user Their names can be modified using the folder to Setup menu Favorites folder USB stick User folders Table 4 6 Overview of the gentleMACS Octo Dissociator with Heaters folders and program transfer rights 4 5 5 Deleting programs To delete a program 1 Select the User folder containing the required program 2 Highlight the program to be deleted 3 Press Delete to remove the program 4 Press OK to confirm or Cancel to abort the process Note Files located in the Miltenyi and Templates folder cannot be deleted Note Deleting a shortcut in the Favorites folder will not delete the original program 4 6 Setup The Setup menu is used to manage the following device settings Set LED switch the LEDs on or off Set time set the time Set date set the date in the format dd mm yyyy Set folder name name rename User folders Software version get information on the software version that is installed on the instrument Serial number get information on the serial number of the instrument 4 6 1 Set LED To set the LED for the instrument 1 Press Set LED 2 Press Set LED on or Set LED off as required 4 6 2 Set clock and date The procedures to modify the time and date are identical
55. g and or sterilization Remove the Trough for cleaning Note To remove the Trough pull on one side of the Trough Avoid pulling on both sides at the same time __ Remove the Saftey Shield Remove the yellow sleeves from the sleeve holders a 4 Remove trough from the baseplate WARNING Optical radiation emitted from gentleMACS Tube and Safety Shield illumination light emitting diodes LEDs may be harmful to the eyes at close viewing distances when the Trough is removed Do not stare at operating LEDs from a distance less than 20 cm when the Trough is removed Read the chapter Important safety information The surface of the gentleMACS Octo Dissociator with Heaters should be cleaned with a cloth soaked with a detergent containing aqueous solution or 70 ethanol Carefully wipe all parts Note For cleaning wipe the gentleMACS Heaters carefully with a soft cloth soaked with 70 ethanol Do not use any detergents Do not soak the gentleMACS Heaters directly into any cleaning solution Repeat the procedure with another cloth soaked with distilled water If the homogenized substances are not water soluble it is recommended to wipe all parts with ethanol Contact Miltenyi Biotec Technical Support for further advice 8 If necessary exchange the Sleeves and the Trough 9 Reassemble the parts while ensuring that the removed parts snap into position correctly Ensure that the ori
56. haben wenden Sie sich an einen autorisierten Miltenyi Biotec Servicedienstleister oder Ihren lokalen Stromversorger Benutzen Sie keine Verl ngerungskabel oder Steckdosenleiste berlasten Sie eine Steckdose nicht IT FR ES oe EN WICHTIGE SICHERHEITSINFORMATIONEN 20 Mechanische Gefahren Bewegliche und rotierende Teile sind potentielle mechanische Gefahrenquellen Benutzen Sie das Ger t nicht ohne befestigtes Trough Sleeves und Safety Shield Wenn sich die Rotoren des Ger tes bewegen ber hren Sie diese nicht und laden oder entfernen Sie keine gentleMACS Tubes vom Ger t Benutzen Sie nur korrekt installierte gentleMACS Tubes mit fest verschlossenem Deckel Benutzen Sie das Ger t nicht mit nur aufgesteckten Deckeln der gentleMACS Tubes Die freiliegenden scharfen Kanten der Deckel der gentleMACS Tubes k nnen Schnittwunden verursachen wenn diese im Betrieb ber hrt werden Umgehen Sie keine Schutzma nahmen oder Sicherheitsvorrichtungen Chemische und biologische Gefahren Abh ngig von dem benutzten biologischen Material kann eine Kontamination oder Infektion zu schweren Verletzungen oder Tod f hren Klinische Proben m ssen als potentiell infekti s angesehen werden Wird oder wurde mit biologischen Gefahrstoffen gearbeitet muss der Bediener des Ger tes entsprechend den f r die verwendeten Substanzen geltenden Warnhinweisen und Schutzbestimmungen eine pers nliche Schutzausr stung tragen Der oben genannte Sicherheits
57. hinweis gilt auch f r etwaige gef hrliche Chemikalien einschlie lich toxischer oder korrosiver Chemikalien tzender oder radioaktiver Substanzen die in der Probe anwesend sein k nnen Tragen Sie Schutzhandschuhe Schutzkleidung und Schutzbrille um Ber hrung der Gefahrstoffe mit Haut und Augen zu vermeiden Mangelhafte oder unzureichende Schutzausr stung ist gef hrlich Werden Gefahrstoffe oder unbekannte Substanzen eingesetzt muss das Ger t unter einem Abzug oder einer Sicherheitswerkbank benutzt werden Falls Gefahrstoffe verwendet wurden oder ausgetreten sind muss auf eine sorgf ltige Dekontamination des Ger tes geachtet werden Es ist strengstens verboten kontaminierte Ger teteile oder Zubeh rteile weiter zu verwenden R hrchen und alle weiteren Verbrauchsmaterialien die in Kontakt mit biologischen Gefahrstoffen gelangt sind m ssen vor dem Entsorgen dekontaminiert werden Alle fl ssigen und festen Abf lle m ssen als Gefahrstoffe angesehen werden Die Handhabung der Abf lle muss daher unter Beachtung der allgemein geltenden Laborsicherheits bestimmungen erfolgen Die Entsorgung der Verbrauchsmaterialien muss gem den rtlichen Bestimmungen erfolgen Gefahr durch optische Strahlung Das Ger t ist mit leistungsstarken lichtemittierenden Dioden LEDs ausgestattet welche das Safety Shield und die gentleMACS Tubes beleuchten Gem der internationalen Norm IEC 62471 hat dieses Lampensystem einen Gefahrenwert der Exp
58. i lo strumento dalla presa di corrente e si contatti un Miltenyi Biotec service provider autorizzato o Miltenyi Biotec Technical Support Lo strumento stato realizzato per un uso esclusivanente al coperto Se dei fluidi entarno nello strumento potrebbero verificrsi dei corti eletrici scosse elettriche o fuoco Si faccia particolare attenzione quando si ha a che fare con sostaze liquide Si faccia in modo che le stesse non fluiscano internamente all apparecchio Si protegga l apparecchio da fuoriuscite improvvise o getti d acqua Si puliscano immediatamente le fuoriuscite Non si faccia uso dello strumento se delle sostanze liquide sono venute a contatto con lo stesso Non si usi l apparecchio in un ambiente umido o con vapore Si evitino aree con elevata umidita o rischio di condensazione Spostrare lo strumento da un ambiente freddo come p e una stanza fredda a 5 C potrebbe causare condensazioni interne allo stesso In tal caso si aspetti che lo stesso si deumidifichi prima di renderlo nuovamente operativo Si stacchi lo strumento dalla presa elettrica prima di pulirlo Non si usino liquidi o prodotti di pulizia aerosol si usi sempre un panno umido Si garantisca un adeguato ricircolo d aria nella stanza dove amp in uso lo strumento Nel caso di assenza di un adeguata circolazione l aria dell ambiente circostante potrebbe non raffreddare lo strumento ad un acccettabile temperatura operativa Si permetta una circolazione sufficient
59. ialmente infecciosas Si se ha usado material de riesgo biol gico el operador debe elegir y llevar puesto equipamiento personal de seguridad como indicado en las advertencias y precauciones para la substancia particular La precauci n de seguridad anterior tambi n es v lida para cualquier qu mico de riesgo incluyendo qu micos t xicos o corrosivos cidos o substancias radioactivas que pueden estar presentes en la muestra Lleve guantes ropa y gafas de seguridad para prevenir el contacto de la substancia con la piel y ojos Un equipamiento de seguridad defectuoso o inadecuado es peligroso El instrumento debe ser utilizado con una cubierta de seguridad si est n siendo procesados materiales peligrosos o desconocidos Si se ha utilizado o derramado material peligroso debe tenerse cuidado en descontaminar a fondo el instrumento Est estrictamente prohibido continuar utilizando accesorios o piezas del instrumento contaminadas Los tubos y otros consumibles que han estado en contacto con material de riesgo biol gico deben ser descontaminados antes de su eliminaci n Todos los residuos l quidos o s lidos deben ser considerados peligrosos de acuerdo con las precauciones universales de laboratorio El desecho de residuos debe hacerse de acuerdo con las regulaciones locales Riesgos de radiaci n ptica El instrumento est equipado con poderosos diodos de emisi n de luz LEDs para iluminar la Safety Shield y los gentleMACS Tubes De acu
60. in replacement from Miltenyi Biotec Der Einsatz tr gt folgendes Sicherheitssymbol Farblich abgesetzte Sicherheits markierung Sicherheits symbol Figure 1 Sicherheitssymbol auf dem Einsatz des gentleMACS Octo Dissociator with Heaters Die Sleeves tragen eine farblich abgesetzte Sicherheitsmarkierung Heizfolie Sicherheits symbol Figure 2 Sicherheitssymbol auf den gentleMACS Heaters IT FR ES oe EN WICHTIGE SICHERHEITSINFORMATIONEN Die Aufsteckh lsen tragen eine farblich abgesetzte Sicherheitsmarkierung Diese Sicherheitsmarkierung erm glicht die Rotation der Aufsteckh lsen optisch wahrzunehmen Alle Sicherheitsaufkleber und Markierungen m ssen sauber und lesbar bleiben berpr fen Sie die Aufkleber regelm ig und ersetzen Sie diese sollten sie aus sicherem Abstand nicht mehr lesbar sein Kontaktieren Sie Miltenyi Biotec um Ersatzaufkleber zu erhalten Allgemeine Sicherheitshinweise Falls Ihr Ger t nicht einwandfrei funktioniert und oder Anzeigen auf dem Display Sie dazu auffordern den technischen Kundendienst zu kontaktieren ist die Betriebssicherheit des Ger tes nicht l nger gew hrleistet Schalten Sie das Ger t sofort aus ziehen Sie den Netzstecker und kontaktieren Sie einen autorisierten Miltenyi Biotec Servicedienstleister oder den Miltenyi Biotec Technical Support Elektrische Gef hrdung und Brandgefahr Elektrische Ger te bergen das Risiko eines Stromschlags Kurzschl
61. iotec Inc 2303 Lindbergh Street Auburn CA 95602 USA Phone 800 FOR MACS Phone 1 530 888 8871 Fax 1 530 888 8925 macs miltenyibiotec com Australia Miltenyi Biotec Australia Pty Ltd Unit 16A 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone 61 2 8877 7400 Fax 61 2 9889 5044 macs miltenyibiotec com au Benelux Miltenyi Biotec B V Schipholweg 68 H 2316 XE Leiden The Netherlands macs miltenyibiotec nl Customer service The Netherlands Phone 0800 4020120 Fax 0800 4020100 Customer service Belgium Phone 0800 94016 Fax 0800 99626 Customer service Luxembourg Phone 800 24971 Fax 800 24984 China Miltenyi Biotec Technology amp Trading Shanghai Co Ltd Room 2309 No 319 Xianxia Road Changning District 200051 Shanghai P R China Phone 86 21 62351005 Fax 86 21 62350953 macs miltenyibiotec com cn France Miltenyi Biotec SAS 10 rue Mercoeur 75011 Paris France Phone 331 56 98 16 16 Fax 331 56 98 16 17 macs miltenyibiotec fr Italy Miltenyi Biotec S r l Via Persicetana 2 D 40012 Calderara di Reno BO Italy Phone 39 051 6 460 411 Fax 39 051 6 460 499 macs miltenyibiotec it Japan Miltenyi Biotec K K Nittsu Eitai Building 5F 16 10 Fuyuki Koto ku Tokyo 135 0041 Japan Phone 81 3 5646 8910 Fax 81 3 5646 8911 macs miltenyibiotec jp Nordics and Baltics Miltenyi Biotec Norden AB Scheelev gen 17 223 70 Lund Sweden macs miltenyibiotec se Customer servic
62. irm The status of the tube position will change to Ready Programs Tube Date m_brain_02_01 C 01 Mar 2011 C 01 Mar 2011 69 BE INSTRUMENT OPERATION 4 Press Start to commence the program The status of the tube position will change to Running Note After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start The program will immediately start and the status of the tube position will change to Running Programs Tube Date m_brain_02_01 5 While Sample A is still running insert Sample B into a free position attach a gentleMACS Heater to the selected position as described in section 4 4 2 1 and select the program 37 C_m_tumor_01 as described in steps 2 and 3 6 Press OK and Start to commence processing of Sample B Programs Tube Date Cc m_neoheart_01_01 C 19 Apr 2013 7 While Sample A and Sample B are running insert Sample C into the next free position and select the program C_01 from the Templates folder Press OK and Start to commence processing of Sample C Programs Tube Date y h_tumor_03_01 C 01 Mar 2011 m_brain_02_01 C__01 Mar 2011 70 8 Remove the samples when the status of the tube positions changes to Done In this example Sample A and Sample C can be removed while Sample B is still running Programs y h_tumor m_bre z lt a LU a o E zZ Ww cc E n D 71 72 4 5 Editor menu The Editor menu is used
63. ith Heaters ist f r einen sicheren Betrieb ausgelegt wenn das Ger t richtig installiert von geschultem Personal bedient und entsprechend der Sicherheitsrichtlinien und Anweisungen in diesem Benutzerhandbuch eingesetzt wird Die in diesem Kapitel enthaltenen Sicherheitsrichtlinien erl utern die mit dem Betrieb dieses Ger tes verbundenen m glichen Gefahren und geben wichtige zus tzliche Informationen zur Sicherheit um diese Risiken zu minimieren Befolgen Sie diese Sicherheitshinweise sorgf ltig um sich andere und das Ger t vor m glichen Gefahren zu sch tzen und ein sicheres Arbeitsumfeld zu schaffen Um Sach und Personensch den zu vermeiden benutzen Sie das Ger t ausschlie lich nach Angaben des Herstellers rtliche Arbeitsschutzbestimmungen Laborrichtlinien Sicherheitsnormen und Unfallverh tungsvorschriften m ssen jederzeit beachtet und eingehalten werden Wenden Sie sich f r weitere Informationen zur Stromversorgung Geb udeinstallationen Wartung und Sicherheit f r die Installation dieses Ger tes an die rtlichen Beh rden und Ihren Stromversorger Gefahrensymbole werden verwendet um auf Sicherheits und Sachschadenhinweise aufmerksam zu machen Folgende Gefahrensymbole werden in diesem Benutzerhandbuch benutzt oder WARNING bezeichnet eine Gefahrensituation die falls sie nicht vermieden wird zum Tode oder schwerwiegenden Verletzungen f hren kann 16 ebbbb gt b gt O 9 oder CAUTION bezeichne
64. iziert und empfohlen werden Die Verwendung unautorisierter Ersatzteile kann Fehlfunktionen des Ger tes verursachen und die Ergebnisse beeintr chtigen Die Miltenyi Biotec GmbH akzeptiert keinerlei Garantieanspr che und haftet nicht f r Fehlfunktionen oder Sch den am Ger t die auf Verwendung ungeeigneter Verschlei oder Ersatzteile zur ckzuf hren sind Stellen Sie sicher dass nach jeder erfolgten Wartungs oder Reparaturleistung ein autorisierter Miltenyi Biotec Servicedienstleister alle notwendigen Sicherheitspr fungen welche die durchgef hrten Reparaturma nahmen verlangen durchf hrt um sicherzustellen dass das Ger t sich in vorschriftsm igem Zustand befindet Nutzen Sie nur von Miltenyi Biotec empfohlenes Zusatzger t und Upgrades zu Ihrem Ger t Fragen Sie Ihren rtlichen Miltenyi Biotec Vertriebsmitarbeiter nach Miltenyi Biotecs weit reichenden Vereinbarungen zum Ger teservice und Technical Support oder besuchen unsere Website www miltenyibiotec com support Das Ger t sollte vorsichtig gehandhabt in der von Miltenyi Biotec bereit gestellten Verpackung transportiert werden Im Ger t k nnen innere Sch den auftreten falls es gro er Ersch tterung ausgesetzt oder fallengelassen wird Sollte wegen Reparatur oder Wartungsleistungen ein R cktransport zum Hersteller notwendig sein dekontaminieren Sie das Ger t vor dem Versand um jegliche Gefahrenstoffe zu entfernen Wenn Sie Fragen zur vorschriftsm igen Dekont
65. k nnen zu H rsch den f hren Es wird empfohlen dass der Betreiber den Schalldruckpegel am Benutzerstandort und am Ort des h chsten Schalldruckpegels im Umkreis von 1 m des Ger tes misst und berechnet Es sollten sch tzenden Ma nahmen wie z B schalld mpfende L rmschutzvorrichtung L rmschutzhauben oder Geh rschutz benutzt werden wenn w hrend der Installation des Ger tes nicht sichergestellt werden kann dass der Schalldruckpegel des Ger tes am Standort der Benutzung einen den die Gesundheit gef hrdenden Wert nicht bersteigt Wartung Transport und Ger teentsorgung Versuchen Sie nicht das Ger t selbst zu warten oder zu reparieren es sei denn es ist in diesem Benutzerhandbuch oder anderen technischen Unterlagen der Miltenyi Biotec GmbH ausdr cklich vermerkt Wartung und Reparaturen m ssen durch geschulte Fachkr fte ausgef hrt werden Falsche oder unsachgem e Wartung oder Reparatur an Ihrem Ger t kann zur Gef hrdung des Anwenders unvorhersehbaren Resultaten Fehlfunktionen Ger tesch den 21 IT FR ES oe EN WICHTIGE SICHERHEITSINFORMATIONEN 22 vorzeitigem Verschlei und verringerter Lebensdauer f hren Es kann auch den Verlust Ihrer Garantieanspr che zur Folge haben Wenn ein Austausch oder Ersatzteile ben tigt werden stellen Sie sicher dass Ihr Servicedienstleister nur Originalteile der Miltenyi Biotec GmbH oder Teile von Drittanbietern verwendet die von der Miltenyi Biotec GmbH spezif
66. l or visit www miltenyibiotec com Design and specifications are subject to change without notice 95 E TECHNICAL SPECIFICATIONS 96 For technical support please contact your local Miltenyi Biotec representative or the Technical Support team at Miltenyi Biotec headquarters Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 830 Fax 49 2204 8306 89 macstec miltenyibiotec de Visit www miltenyibiotec com local to find your nearest Miltenyi Biotec contact 97 98 Except as stated in a specific warranty statement which may accompany this product or unless otherwise agreed in writing by an authorized representative of Miltenyi Biotec Miltenyi Biotec s warranty if any with respect to this product is subject to the terms and conditions of sale of the company within the Miltenyi Biotec group which supplied the product Terms and conditions of sale may vary by country and region Nothing in this document should be construed as constituting an additional warranty Miltenyi Biotec s warranty for this product only covers product issues caused by defects in material or workmanship during ordinary use it does not cover product issues caused by any other reason including but not limited to product issues due to use in a manner other than specifically described in this manual for example inappropriate or improper use incorrect assembly or installation by an operator or a third par
67. le misure indicate dall organismo responsabile o il calcolo dell livello della pressione sonora in prossimit della posizione dell operatore ed a una qualunque posizione ad 1 m dalla zona dove si trova l attrezzatura che abbia il pi alto livello di pressione sonora Se non viene verificato durante l installazione che il livello di pressione del suono dal dispositivo non raggiunga un valore tale durante il suo uso che possa causare dei rischi allora deve essere impiegato del materiale protettivo come pannelli acustici cappe o tappi per le orecchie Manutenzione e trasporto A meno che non differentemente specificato in questo manuale o in altra documentazione Miltenyi Biotec non si faccia la manutenzione dello strumento da soli La manutezione e le riparazione devono essere condotte da personale di servizio qualificato Una manutenzione impropria o scorretta ovvero riparazioni dello strumento possono causare pericolo per gli utenti o portare a danni irreparabili causare un malfunzionamento dello strumento o danni nonch usura prematura e ridotta vita utile dello stesso Potrebbe inoltre fare cadere la Vostra garanzia Sostituendo componenti o se sono necessari pezzi di ricambio assicurarsi che il service provider usi solo parti originali Miltenyi Biotec o altrimenti terze parti consigliate e raccomandate da Miltenyi Biotec L utilizzo di parti non originali pu causare malfunzionamenti dello strumento e risultati imprecisi Miltenyi Biote
68. ly Copyright 2014 Miltenyi Biotec GmbH All rights reserved 140 004 260
69. ly and or the displayed instructions or messages advise you to contact the technical support safe operation ofthe instrument is not longer possible Immediately switch off and unplug the instrument from the power outlet and contact an authorized Miltenyi Biotec service provider or Miltenyi Biotec Technical Support Electricity and fire hazards Potential risks arising from electrical devices include electric shock short and overheating Electric shock may lead to severe personal injury or even death An electric short or overheating may cause a fire Electronic equipment might emit sparks which could ignite combustible vapors or material resulting in explosion or fire Do not use the instrument in areas classified as hazardous locations for example in oxygen laden environments The housing of the instrument is designed to reduce the risk of electric shock electric short and spread of fire Except for the Safety Shield and Trough do not remove or penetrate any cover Only authorized personnel must remove the other covers of the instrument Never push a foreign object through an opening into the instrument Do not use the instrument if itis opened or disassembled it has been dropped or damaged it has damaged or broken parts it has a damaged power cable e an object has entered through the ventilation slots e a foreign object has been dropped into the instrument If flames or smoke appear immediately switch off the powe
70. ntleMACS Octo Dissociator with Heaters also allows users to create new programs This can be accomplished afresh or by modifying pre defined templates Please unpack the instrument carefully and confirm that all components have been successfully delivered without damage that may have occurred during transportation WARNING If there is any damage do not use the instrument Contact your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support Operating a damaged instrument may cause mechanical hazards optical radiation hazards fire hazards or electric shock Read the chapter Important safety information before installing and assembling the instrument Please retain the original packaging material The following components are included in the delivery ofthe gentleMACS Octo Dissociator with Heaters 130 096 427 1x gentleMACS Octo Dissociator with Heaters 8x gentleMACS Heaters 1x Power cord 1x User manual 53 54 2 2 Installation Read the instructions in the chapter Important safety information and ensure that the site is properly prepared before continuing with installation and assembly Incorrect installation can lead to the spread of fire explosion the risk of electric shock or mechanical hazards Note The gentleMACS Octo Dissociator with Heaters is a ready to use instrument that requires a power connection and a flat surface of the dimensions 465x285 mm plus at least 15 cm on all sides to allow suffi
71. nzionate nell etichetta di indicazione elettrica del prodotto Nel caso ci fossero domande in merito al tipo di fontte energetica si contatti il service provider Miltenyi Biotec autorizzato o la compagnia energetica di fornitura locale Non si usino prolunghe o prese multiple Non si sovraccarichi una presa elettrica Rischi meccanici Spostare o far ruotare le parti potrebbe causare rischi di natura meccanica Quando si fa uso dello strumento si mantengano collegati i Trough i Sleeves e i Safety Shield ad esso connessi Mentre i rotori dello strumento sono in funzione non li si tocchi non li si carichi o si rimuovano le provette dall appaecchio Si usino solamente i gentleMACS Tubes opportunamente installati con chiusure ermetiche Non si faccia funzionare l apparecchio con solo i tappi dei gentleMACS Tubes in posizione bordi taglienti dei tappi dei gentleMACS Tubes potrebbero causare 45 m FR ES DE EN IMPORTANTI ISTRUZIONI DI SICUREZZA 46 ferite se toccati mentre in rotazione Non si aggirino le misure di sicurezza o si usino diversi dispositivi Rischi chimici e biologici In base al materiale biologico impiegato alla contaminazione o all infezione possono verificarsi gravi lesioni a persone o anche la morte Tutti i casi clinici devono essere considerati come potenzialmente infetti Se un materiale a rischio biologico o stato in uso l operatore deve scegliere ed indossare delle protezioni di sicurezza person
72. o en el interior del instrumento Esta informaci n es para el personal de servicio ON Fuente de energ a OFF Fuente de energ a Lea todas las instrucciones de uso antes de instalar y utilizar el instrumento Etiqueta de seguridad La siguiente etiqueta de seguridad se encuentra pegada a la parte posterior del instrumento y juega un papel importante en el mantenimiento de la seguridad Avoid serious injury Read and understand operating instructions before installation or use If manual is missing obtain replacement from Miltenyi Biotec La base presenta los siguientes simbolos de seguridad Impresi n de contraste para seguridad Simbolo de seguridad Figure 1 Simbolo de seguridad en la base del gentleMACS Octo Dissociator with Heaters Los manguitos est n marcados con una impresi n de contraste para seguridad L mina calefactada Simbolo de seguridad Figura 2 Simbolo de seguridad en los calefactores gentleMACS Los manguitos est n marcados con una impresi n de contraste para seguridad Esta impresi n de contraste para seguridad permite la percepci n visual de los manguitos rotatorios 27 IT FR Es DE EN INFORMACI N IMPORTANTE DE SEGURIDAD 28 Las etiquetas de seguridad arriba mencionadas y las marcas de seguridad deber n mantenerse limpias y legibles Inspecionar peri dicamente las etiquetas y marcas de seguridad y reemplazarlas en caso de que estas no sean legibles
73. o perceptibles a una distancia visual segura Contacte con Miltenyi Biotec para reemplazo de etiquetas Instrucciones generales de seguridad Si el instrumento no funciona correctamente y o las instrucciones o mensajes mostrados le aconsejan contactar con el servicio t cnico no es posible utilizar el instrumento de una manera segura Apague y desenchufe inmediatamente el instrumento de la toma de corriente y contacte un proveedor de servicios autorizado de Miltenyi Biotec o un servicio t cnico autorizado Incendios y riesgos el ctricos Los riesgos potenciales causados por aparatos el ctricos incluyen shock el ctrico cortocircuito y sobrecalentamiento Un shock el ctrico puede llevar a graves da os corporales o incluso la muerte Un cortocircuito el ctrico o sobrecalentamiento puede ser causa de incendio El equipo electr nico puede emitir chispas las cuales podr an encender vapores o material combustible resultando en explosi n o incendio No use el instrumento en reas designadas como peligrosas por ejemplo en medios cargados de ox geno La caja protectora del instrumento est dise ada para reducir el riesgo de shock el ctrico cortocircuito el ctrico y propagaci n de fuego Excepto la de la Safety Shield y la Trough no quite o penetre ninguna tapa o cubierta Solo el personal autorizado debe quitar las otras tapas del instrumento Nunca introduzca un objeto extra o a trav s de una apertura en el instrumento No use el
74. of the cap and the tube must be perfectly clean for proper functioning of the seal Replace the tube Exchange sleeve Refer to the respective data sheet for technical specifications of the tubes Process a smaller amount of tissue or use a different gentleMACS Program Check if tissue is trapped between stator and rotor Release tissue reinstall tube and press continue to restart the program Ensure correct installation of the tube Close the tube tightly beyond the first resistance 89 90 A warning message appears in a pop up window when saving an edited program Input error Maximum rounds per run 3 200 exceeded New program containing iterations cannot be saved The instrument displays an error message with the cause of the error A warning message the heater was removed appears in a pop up window while running a heater program The duration of the program exceed 3 200 rounds per run rpr It is not recommended to run programs exceeding this limitation Miltenyi Biotec does not guarantee proper function of the instrument and disposables above this value Loop command is faulty End loop command is missing indicates that the end loop command has not been specified Loop command is faulty The command is nested indicates that there are more than one loop commands Loop command is faulty Loop has no body indicates that there is no c
75. ommand between the loop and end loop commands The heater was removed The heater is not installed properly The electrical contacts of the instrument or the heaters are dirty or corroded Press OK to save the program Press Abort to reedit the program Close iteration by adding an end loop command Use only one loop command and close the iteration by adding an end loop command At least one program step must be included between a loop and end loop command Add steps and define commands that shall be iterated Reinstall heater Make sure that the heater is installed properly Carefully clean the heater contacts of the instrument and the erps Heater using a cloak soaked with water or 70 ethanol Carefully check the heater for signs of damage If no visible signs of damage can be detected switch the instrument OFF and ON again and retry to run the program Contact Technical Service Program is assigned Program contains a to a tube position and heating step and no heater displays ared error is installed message A warning message The heater was removed the heater was removed appears The heater is not installed in a pop up window properly while running a heater program The electrical contacts ofthe instrument or the heaters are dirty or corroded Shortcuttoaprogram The Favorites folder has cannot be created in reached the maximum the Favorites folder limit of 20 shortcuts The
76. ommand is added namely a clockwise spin at 2 000 rpm for 10 seconds Main menu editor Setup 13 06 000 rpm 10 Note Loop commands enable the repetition of program steps The iterations will only include the steps between a loop and end loop command In this example the two spin commands shall be repeated three times 4 Highlight the first command and press Add to select the next command Select loop to mark the beginning of an iteration Enter the number of iterative cycles In this example 3 cycles are chosen Main menu Editor Setup 13 07 new_program in 2000 rpm 1 in 2000 rpm 10 d end loop 5 Click OK to confirm the selection The command loop and the number of cycles are displayed as a new step 75 BE INSTRUMENT OPERATION 76 6 Highlight the end command and press Add Select end loop to close the iterative cycle new_program pm 1 000 rpm 10 7 Press OK to confirm 8 Highlight the first command and press Add to insert another command Select ramp to reach a certain speed in a defined time 9 Enter speed and time Press OK to confirm the values In this example a ramp of 2 000 rpm in 10 seconds has been added Main menu Editor setup 13 09 new_program 1 loop 3 x spin in 2000 rpm 10 loop 4 end loop teed end loop 10 Highlight the end command and press Add Select temp and ON from the pop up screen to mark the beginning of the heating period Press
77. onnel qualifi conform ment aux mesures de s curit g n rales et aux consignes libell es dans ce manuel Les directives dans ce chapitre d crivent les risques potentiels associ s au maniement de cet appareil et fournissent d importantes informations de s curit suppl mentaires pour r duire les risques Suivez soigneusement les instructions afin de vous prot ger vous m me des tiers et le dispositif d ventuels dangers et de cr er un environnement de travail s r Utilisez cet appareil uniquement en respectant les instructions du fabricant afin d viter une d t rioration du dispositif et des blessures du personnel Suivez toujours les consignes de s curit du champ d op ration et les bonnes pratiques de laboratoire ainsi que les normes pour la sant la s curit et la pr vention d accidents Contactez les autorit s locales g rant l alimentation en courant lectrique la construction de b timents la maintenance ou la s curit pour plus d informations sur l installation et l utilisation s re de l appareil Des mots cl sont utilis s pour identifier des messages sur des d t riorations mat riels et de s curit Les mots cl suivants sont utilis s dans ce manuel ou WARNING indique une situation potentiellement dangereuse pouvant entra ner la mort ou des blessures graves si elle n est pas vit e ou CAUTION indique une situation potentiellement dangereuse pouvant causer des blessures mineures ou mod
78. osition EHV von 0 91 und f llt in die Freie Gruppe Der Gef hrdungsabstand HD f r die Freie Gruppe betr gt 61 cm Der Gef hrdungsabstand f r die Risikogruppe 1 betr gt 20 cm Das Risiko f r den Betrachter ist abh ngig von der Installation und Benutzung des Ger tes Die von den LEDs emittierte optische Strahlung kann gef hrlich f r die Augen sein wenn man aus sehr geringer Entfernung in das Ger t mit entferntem Trough schaut Ziehen Sie den Netzstecker des Ger tes bevor Sie den Trough entfernen Wenn der Trough w hrend des Betriebs vom Ger t entfernt wird blicken Sie aus einer Entfernung von weniger als 20 cm nicht direkt in das Licht der LEDs Ferner werden LEDs f r die Beleuchtung des Lesebereiches eines integrierten 2D Code Leseger ts benutzt Blicken Sie im Betrieb nicht aus kurzer Entfernung direkt auf das 2D Code Leseger t Nehmen Sie optische Strahlungsquellen nicht auseinander Ver ndern oder entfernen Sie diese nicht Die optische Strahlung aus ge ffneten Ger ten kann f r die Augen sch dlich sein Gefahr durch hei e Oberfl che Die Heizfolie kann ausreichend hei werden um bei Ber hrung Verbrennungen zu verursachen Die Heizfolie darf nicht ber hrt werden w hrend das Ger t in Betrieb ist Gefahr durch Ger uschentwicklung Wird das Ger t bei maximaler Beladung benutzt kann es zu einer Ger uschentwicklung kommen dessen Pegel eine Gesundheitsgef hrdung darstellt Gef hrliche Schalldruckpegel
79. ppareil est utilis une charge maximale il peut produire des bruits un niveau dangereux Des niveaux de bruits dangereux peuvent entra ner une d faillance auditive Il est recommand que le corps responsable mesure ou calcule le niveau de la pression acoustique la position de l op rateur Il doit galement d finir quel point un m tre du bo tier du dispositif l on mesure le plus haut niveau de pression acoustique S il n est pas garanti pendant l installation que le niveau de pression acoustique de l appareil n atteindra pas une valeur pouvant entra ner un danger son endroit d utilisation des mat riels de protection tels que des baffles ou hottes r duisant le bruit ainsi que des protections sonores doivent tre utilis es Maintenance transport et limination N entretenez pas vous m me l appareil moins que ceci ait t sp cifiquement mentionn dans ce manuel ou d autres documentations de Miltenyi Biotec La maintenance et les r parations doivent tre effectu es par un personnel de maintenance qualifi Une maintenance ou r paration impropre ou incorrecte de l appareil peut causer des dangers pour l utilisateur amener des r sultats impr visibles causer un dysfonctionnement ou une d t rioration de l appareil ainsi qu une usure pr matur e et une r duction de la long vit de l appareil Ceci peut galement faire expirer votre garantie Si des pi ces de rechange sont n cessaires assurez
80. r es si elle n est pas vit e Il peut galement servir d alerte contre une utilisation risqu e du gentleMACS Octo Dissociator with Heaters 33 34 PPPPP gt Symb les de s curit Les symboles suivants sont utilis s pour souligner des conditions qui pourraient entra ner des blessures du personnel ou une d t rioration du dispositif Alerte de s curit Risque de danger La documentation doit tre consult dans les cas o un symbole d alerte de s curit est mentionn sur l instrument afin de connaitre la nature du danger potentiel et les mesures prendre Danger biologique Risque de contamination si des mati res biologiques potentiellement dangereuses sont utilis es Risque d un choc lectrique Radiation optique dangereuse Risque de capture involontaire d un doigt par rotation d une partie dangereuse Surface dangereusement chaude Borne pour conducteur de protection Ce symbole est fix l int rieure de l appareil Cette information est destin e au personnel de service ON Alimentation lectrique OFF Alimentation lectrique Les instructions de service doivent tre lues dans leur int gralit avant l installation et l utilisation de l appareil tiquette de s curit L tiquette de s curit est fix e l arri re de l appareil et joue un r le important pour la maintenance de s curit A WARNING Avoid serious injury Read and understand operating instructions
81. r supply unplug the instrument from the electrical outlet and contact an authorized Miltenyi Biotec service provider or Miltenyi Biotec Technical Support The instrument is intended for indoor use only If fluids enter the instrument it may lead to electric shorts electric shock or fire Take special care while handling fluids Do not allow fluids to enter the interior of the instrument Protect the instrument against accidental spillages and splashes Clean up spillages immediately Do not operate the instrument if liquids have spilled into the instrument Do not use the instrument in a wet or damp location Avoid areas with high humidity or condensation Moving the instrument from a cold environment such as a cold room at 5 C to room temperature may cause condensation inside the instrument In such cases wait for the instrument to dehumidify before operating it Unplug the instrument from the electrical outlet before cleaning Do not use liquid or aerosol cleaning agents always use a damp cloth Ensure adequate air circulation in the room where the instrument is operated In the absence of adequate circulation ambient air may not cool the instrument to acceptable operating temperatures Allow sufficient air circulation around the instrument at least 15 cm on all sides during operation to ensure adequate cooling Do not place the instrument near radiators heat registers stoves or other equipment including amplifiers that gener
82. rer to avoid damage of equipment and injury to personnel Always follow local working area safety instructions and laboratory policies as well as the standards for health safety and prevention of accidents Contact the local authority governing electrical power supply building constructions maintenance or safety for more information about the safe installation and operation of the instrument Signal words are used to identify safety and property damage messages The following signal words are used throughout this user manual or WARNING indicates a potentially hazardous situation which if not avoided could result in death or serious injury or CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices ebpbbb gt gt 9 Safety symbols The following symbols are used to highlight conditions that could cause injury to personnel or damage to equipment Safety alert Risk of danger The documentation needs to be consulted in all cases where this safety alert symbol is used on the device in order to find out the nature of the potential hazard and any actions that have to be taken Biohazard Risk of contamination if potentially dangerous biological material is used Risk of electric shock Hazardous optical radiation Risk of unmeant trapping of finger by hazardous rotating part Hazardous hot surface Protec
83. rmation Please dispose of your end of life Miltenyi Biotec products in accordance with the applicable WEEE and hazardous waste disposal legislation which may differ by country or region Electrical and electronic equipment may contain hazardous substances which may have a serious detrimental effect on the environment and or human health That is why all equipment must be specifically collected and treated by designated waste facility centres and by qualified WEEE compliance schemes By ensuring that you dispose of your unwanted electrical and electronic equipment according to the applicable WEEE and hazardous waste disposal legislation you are helping to preserve our natural resources and protect human health Miltenyi Biotec is committed to protecting the environment Miltenyi Biotec offers product end of life return programs in many countries and partners with licensed WEEE compliance schemes throughout the world Miltenyi Biotec lets you recycle your end of life Miltenyi Biotec equipment free of charge The terms and availability of this offer vary by geography because of differences in regulatory requirements Please note that depending on the type and use of your equipment additional requirements may apply For more information or if you wish to dispose of your end of life Miltenyi Biotec equipment please contact your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support Der gentleMACS Octo Dissociator w
84. rogram_03 Exit Delete 3 Press Save 79 BE INSTRUMENT OPERATION 80 4 Select the Favorites folder in the pop up window Confirm by pressing OK Main menu Favorites Miltenyi USB stick x Y Exit Delete Add Edit 4 5 4 Transfering programs to other folders Programs can be transferred across folders and USB drives Refer to table 4 6 for an explanation of program transfer rights Note The Favorites folder contains only links to programs in other folders and cannot be used to transfer programs Note Before transferring programs to a USB stick ensure that the USB stick is inserted securely into the USB port 1 Highlight the folder containing the required program 2 Check the corresponding checkbox to select the program Note that it is possible to select multiple programs In this example three programs in the folder User_1 are selected for transfer Main menu Programs ram_01 3 Press Save 4 Choose the destination folder by pressing the folder icon in the pop up window until the respective folder appears Press OK to save the program or Cancel to abort the process In this example the programs are transferred to a USB stick Main menu MET 5 Open the destination folder to ensure that the transfer was successful Programs Tube Date 2 E lt Ww a o E A Ww cc E n gt 81 Folder Description Program transfer rights Miltenyi This
85. rs are stackable and thus allow filtration with decreasing mesh sizes in one go 55 56 MACS Tissue Dissociation Kits were specifically designed for the convenient and standardized enzymatic dissociation of multiple tissues in combination with the gentleMACS Octo Dissociator with Heaters For a comprehensive list of MACS Tissue Dissociation Kits please refer to www gentlemacs com 3 2 Spare parts Product Description Order no gentleMACS Octo Container for liquid waste 130 096 541 Trough Table 3 2 Spare parts of the gentleMACS Octo Dissociator with Heaters Note Only use spare parts and accessories recommended by Miltenyi Biotec Failure to use recommended spare parts and disposables may compromise safe operation and may result in inaccurate results and reduced life time ofthe instrument Miltenyi Biotec does not honor any warranty or accept any responsibility for damages resulting from the use of inappropriate accessories Read the instructions in the chapter Important safety information before operating the instrument When processing infectious radioactive poisonous or any other hazardous liquids always take the necessary safety precautions e Before using organic solvents or aggressive chemicals in the dissociation or homogenization process the compatibility with the instruments parts and tubes must be tested Do not operate the instrument if the Sleeves the Trough or the Safety Shield are removed For ins
86. se or counter clockwise 100 r s max 150 mNm Overload restriction over the range of 200 4 000 rpm 4 C to 35 C 4 C to 30 C 15 80 relative non condensing Maximum 2 000 m 15 C to 70 C Pin 1 USB 5 V Pin 2 USB Pin 3 USB Pin 4 USB GND 93 94 Technical specifications cont CAN port Pin 1 4 5 8 NC Pin 2 CAN L Pin 3 6 GND Pin 7 CAN H Pin 9 24VDC 1A Number of 8 heatable positions Working 37 C temperature heaters Table 7 1 Technical specifications of the gentleMACS Octo Dissociator with Heaters The gentleMACS Octo Dissociator with Heaters is a Protection Class instrument and must be plugged into a grounded power outlet The main power supply cord and plug of the instrument must comply with following specifications USA and Canada only UL listed and KAM cord minimum type SJ minimum 18 AWG 3 conductors Rated for aminimum temperature of 60 C Provided with grounding type NEMA 5 15P attachment plug rated 125 VAC 10 A Opposite end terminates in IEC 320 style connector rated 125 VAC 10 A Conditions of operation Supply voltage fluctuations up to 10 ofthe nominal voltage Transient over voltages present on the mains supply category Il The instrument is suitable for rated pollution degree 2 This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to Part 15 ofthe FCC Rules These limits are d
87. sition will change to Ready The timer indicates the duration of the program Programs Tube Date y 37C_m_tumor_01 C 19 Apr 8 Press Start to commence the program The status of the tube position will change to Running The progress bar and timer indicate the time remaining Note After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start The program will immediately start and the status of the tube position will change to Running Programs Tube Date 37C_m_tumor_01 C 19 Apr 9 On completion of the program the status of the tube position will change to Done The progress bar and timer display the elapsed time since completion of the program Programs _tumor_01 10 Remove the tube from the tube position The status of the tube position will change to Free 66 4 4 2 2 Running multiple samples simultaneously Up to eight samples can be run simultaneously using the same or different programs In this example eight samples will be processed using the same program Note Do not switch off the instrument while running a program to avoid severe damage of the instrument 1 Attach the tubes upside down to the Sleeves of the free tube positions The status of the tube positions will change from Free to Selected Programs Tube Date y 37C_m_tumor_01 C 19 Apr 2013 2 To select a dissociation program press the Folder icon to select the required folder The list of gentleMAC
88. so secondo le disposizioni di legge riguardanti lo smaltimento di apparecchiature elettriche ed elettroniche WEEE e dei rifiuti pericolosi che possono differire da paese a paese Le apparecchiature elettriche ed elettroniche possono contenere sostanze pericolose che possono avere un grave effetto dannoso per l ambiente e o la salute umana per questo che tutte le attrezzature devono essere specificamente raccolte e trattati da centri di smaltimento abilitati e qualificati in conformit con la normativa WEEE Il corretto smaltimento delle apparecchiature elettriche ed elettroniche secondo le disposizioni di legge WEEE e dei rifiuti pericolosi possono aiutare a preservare le risorse naturali e a tutelare la salute umana Miltenyi Biotec si impegna a proteggere l ambiente Miltenyi Biotec offre programmi per lo smaltimento dei prodotti dismessi in molti paesi e partner accreditati per lo smaltimento secondo le normative WEEE in tutto il mondo Miltenyi Biotec consente di riciclare le apparecchiature Miltenyi Biotec gratuitamente Le condizioni e la disponibilit di questa offerta variano da zona a zona a causa di differenze legislative Si prega di notare che a seconda del tipo e dell utilizzo delle apparecchiature possono essere necessari requisiti aggiuntivi Per ulteriori informazioni o se si desiderassero smaltire le apparecchiature Miltenyi Biotec in disuso si prega di contattare il Responsabile di Zona o il Supporto Tecnico Miltenyi
89. t eine Gefahrensituation die falls sie nicht vermieden wird zu leichteren oder mittelschweren Verletzungen f hren kann Es kann auch verwendet werden um vor unsicherem Gebrauch zu warnen Sicherheitssymbole Die folgenden Symbole werden benutzt um Gefahrensituationen anzuzeigen welche zu Sach und Personenschaden f hren k nnen Warnzeichen Gefahrenrisiko Dieses Benutzerhandbuch muss immer konsultiert werden wenn dieses Warnzeichen auf dem Ger t benutzt wird um mehr ber die m glichen Gefahren und entsprechende Handlungsanweisungen zu erfahren Biologische Gef hrdung Risiko der Kontamination wenn mit m glicherweise gef hrlichen biologischen Substanzen gearbeitet wird Gefahr eines Stromschlags Gef hrdung durch optische Strahlung Gefahr unbeabsichtigten Einklemmens der Finger durch rotierende Bauteile Gef hrdung durch hei e Oberfl che Schutzleiteranschluss Dieses Symbol ist innerhalb des Ger ts angebracht und stellt einen Hinweis f r das Servicepersonal dar AN Stromversorgung AUS Stromversorgung Vor der Installation und Inbetriebnahme des Ger tes ist das Benutzerhandbuch zu lesen Sicherheitskennzeichnung Der folgende Sicherheitsaufkleber ist auf der R ckseite des Ger tes befestigt Seine Beachtung spielt f r die Aufrechterhaltung der Sicherheit eine wichtige Rolle Avoid serious injury Read and understand operating instructions before installation or use If manual is missing obta
90. te Ensure that the tube is securely fastened to its sleeve and socket It should click into position Do not force the tube into the position In case of resistance slightly turn the tube clockwise in order to find the right position to click in Optional If running a program including an incubation step apply a gentleMACS Heater to the respective tube position Slightly open the clamp of the gentleMACS Heater by pressing the upper handles and adjust it over the tube Make sure the lower side of the gentleMACS Heater is facing towards you therefore allowing the electrical contact to close tightly Note Make sure that the gentleMACS Heater is securely fastened around the tube and to the socket Do not use the gentleMACS Heater without a tube To select a dissociation program press the Folder icon to select the required folder Note Programs can be selected from a total of eight folders Favorites Miltenyi Templates and five User folders Press the folder icon several times to scroll through The list of gentleMACS Programs in the respective folder will be displayed Highlight the required gentleMACS Program touching the screen or using the scroll buttons Note Press the column header Programs to sort programs within a folder alphabetically Press the column header Date to sort programs by the date of creation 65 BE INSTRUMENT OPERATION 7 Select the required program and press OK to confirm The status of the tube po
91. ted as close to the operator of the instrument as possible If it is necessary to disconnect the instrument s power supply unplug the cable from the power outlet 4 Setthe switch located above the plug socket to ON on O orr 5 The instrument will perform a start up check that runs for a few seconds The following materials are required to operate the gentleMACS Octo Dissociator with Heaters C Tubes For gentle preparation 25 tubes sterile 130 093 237 of single cell single packed suspensions from tissues for subsequent analysis and cell packed as 4x25 tubes culture M Tubes For homogenization 25 tubes sterile 130 093 236 of tissues and cells for single packed subsequent subcellular d con lysi 100 tubes sterile 130 096 335 SENSE packed as 4x25 tubes M Tubes with For homogenization 50 tubes per bag 130 094 392 Strainer of tissues and cells for subsequent subcellular and molecular analysis The strainer removes aggregates or large particles from the sample strainer 600 um mesh Table 3 1 Disposables required for the gentleMACS Octo Dissociator with Heaters MACS SmartStrainers are filters that are designed for the easy removal of cell aggregates or large particles after tissue dissociation The SmartStrainers are available with three mesh sizes 30 um 130 098 458 70 um 130 098 462 and 100 um 130 098 463 They easily fit into standard 15 and 50 mL conical tubes of all major suppliers Straine
92. thoroughly decontaminate the instrument It is strictly prohibited to continue to handle contaminated accessories or parts of the instrument Tubes and any other consumables that were in contact with biohazardous material must be decontaminated before disposal All liquid and solid waste must be considered hazardous and therefore be handled in accordance with universal laboratory precautions Waste disposal must be in accordance with the local regulations Optical radiation hazards The instrument is equipped with powerful light emitting diodes LEDs for illuminating the Safety Shield and the gentleMACS Tubes According to the international standard IEC 62471 this lamp system has an exposure hazard value EHV of 0 91 and is in excess of the Exempt Risk Group The hazard distance HD for the Exempt Risk Group is 61 cm The hazard distance for Risk Group 1 is 20 cm The viewer related risk is dependent on how the instrument is installed and used The optical radiation emitted from these LEDs may be harmful to the eyes at close viewing distances if the Trough is removed Unplug the instrument before removing the Trough If the Trough is removed do not stare at the operating illumination LEDs from a distance lesser than 20 cm In addition the integrated 2D code reader uses LEDs for illuminating the reading area Do not stare directly at the 2D code reader from a short distance while it is in operation Do not disassemble modify or remove any
93. tion of single cell suspensions from tissues for subsequent cell separation cell analysis or cell culture e Orange M Tubes are typically used for the homogenization of tissues and cells for subsequent subcellular or molecular analyses gentleMACS Tubes allow for the processing of sample volumes from 300 uL to 10 mL and from 20 mg to 4 000 mg of tissue in a single tube sample pipetting through a septum sealed opening in the center of the tube without opening the tube itself e sterile sample handling gentleMACS Tubes consist of a stator and rotor element that provides the exact sheer forces necessary to gently pull cells from tissues or intact molecules from cells A specially engineered tube enclosure always directs the sample flow towards the rotor and stator element The pierceable septum allows for removing the sample without opening the tube This sophisticated design results in highly efficient tissue dissociation and homogenization using C Tubes and M Tubes respectively Tube enclosure Septum seal az gt Rotor Stator INTRODUCTION Figure 1 2 Caps of gentleMACS Tubes exemplary M Tubes orange C Tubes purple a 1 3 Sample preparation combining enzymatic and mechanical dissociation For combined enzymatic and mechanical dissociation processes the instrument comes equipped with gentleMACS Heaters for each of the eight tube positions The gentle
94. tive conductor terminal The symbol is attached to the inside of the instrument This is information for service personnel ON Power supply OFF Power supply All operating instructions must be read before installing and operating the instrument Safety label The following safety label is attached to the back of the instrument and plays an important part in maintaining safety VANTINI Avoid serious injury Read and understand operating instructions before installation or use If manual is missing obtain replacement from Miltenyi Biotec The Trough is marked with the following safety symbol Contrasting safety print Safety symbol Figure 1 Safety symbol on the Trough of the gentleMACS Octo Dissociator with Heaters The Sleeves are marked with a contrasting safety print Heating foil Safety symbol Figure 2 Safety symbol on the gentleMACS Heaters The Sleeves are marked with a contrasting safety print The contrasting safety print allows the visual perception of the rotation of the Sleeves IT FR ES DE Pen IMPORTANT SAFETY INFORMATION 10 The mentioned above safety labels and safety markings must be kept clean and legible Periodically inspect the safety labels and safety markings and replace them if they are not legible or perceptible from a safe viewing distance Contact Miltenyi Biotec for replacement labels General safety instructions If the instrument is not working proper
95. to e create new programs for details refer to section 4 5 1 edit programs for details refer to section 4 5 2 create program shortcuts into the Favorites folder for details refer to section 4 5 3 e transfer programs between folders and to a USB stick and vice versa for details refer to section 4 5 4 Note Programs from the Miltenyi folder are not editable Miltenyi templates from the Templates folder can be edited but must be renamed and saved in one ofthe User folders 4 5 1 Creating a new program There are several optional commands to design a user defined program including options on the temperature rotation speed direction and duration acceleration and iteration Command Description temp ON Defines the beginning of an incubation period Any command between the temp ON and temp OFF command will be performed at 37 C spin Defines the speed direction and duration of rotation Speed is defined as revolutions per minute rpm Prefixing the rpm value with minus results in a counter clockwise rotation The total duration is given in minutes and seconds The following are the speed restrictions Clockwise 20 to 4 000 rpm Counter clockwise 20 to 4 000 rpm The change of velocity must not exceed 6 000 rpm per second loop Defines the beginning of a loop cycle Commands between the loop and end loop commands are repeatedly executed for the defined number of cycles A maximum of
96. trument schematics refer to section 4 2 Do not leave the gentleMACS Heaters attached to the tube position while not in use to avoid wearing of the gentleMACS Heaters Do not switch off the instrument while running a program to avoid severe damage of the instrument The gentleMACS Octo Dissociator with Heaters is used as a stand alone benchtop instrument A number of pre defined programs offer a variety of optimized protocols for the automated dissociation and homogenization of tissues The instrument also allows creation and use of user defined programs for specific applications gentleMACS C or M Tubes are required for processing the samples The gentleMACS Octo Dissociator with Heaters must be used exclusively with these gentleMACS Tubes Moreover the gentleMACS Heaters are specially designed for use with the instrument when running a program that includes an incubation step 57 4 2 Instrument schematics gentleMACS Heaters lt __ Safety Shield Touchscreen Figure 4 1 Front of the gentleMACS Octo Dissociator with Heaters 2D code reader USB port ON OFF switch Power socket Figure 4 2 Back of the gentleMACS Octo Dissociator with Heaters Sleeve Trough CAN port Safety and RS232 port type label Figure 4 3 Back of the gentleMACS Octo Dissociator with Heaters Safety Shield removed 58 4 3 Starting the instrument To start the instrument 1 Ensure that the device is plugge
97. trumento Etichetta di sicurezza La seguente etichetta amp applicata sul retro dello strumento e gioca un ruolo fondamentale nel mantenimento del livello di sicurezza Avoid serious injury Read and understand operating instructions before installation or use If manual is missing obtain replacement from Miltenyi Biotec seguenti simboli di sicurezza sono esposti sul fondo della vaschetta Stampa di sicurezza di colore diverso Simbolo di sicurezza Figure 1 Simbolo di sicurezza sul fondo della vaschetta del gentleMACS Octo Dissociator with Heaters Lamina riscaldante Simbolo di sicurezza Figura 2 Simbolo di sicurezza sul gentleMACS Heaters perni sono evidenziati da una marcatura di sicurezza di colore diverso La funzione delle marcature quella di evidenziare il movimento rotatorio del perno 43 m FR ES DE EN IMPORTANTI ISTRUZIONI DI SICUREZZA 44 Le etichette di sicurezza e i marchi di sicurezza devono essere mantenuti puliti e leggibili Ispezionare periodicamente le etichette di sicurezza e i marchi di sicurezza e sostituirli se illegibili o percettibili da una distanza di sicurezza Contattare Miltenyi Biotec per delle etichette sostitutive Istruzioni generali di sicurezza Se lo strumento non funziona correttamente e o le istruzioni visualizzate dei messaggi di avviso suggeriscono di contattare l assistenza tecnica non pi possibile un uso sicuro dello strumento
98. ts doit tre conforme aux r glementations locales Dangers de radiation optique L appareil est quip de puissantes diodes lectroluminescentes DELs pour illuminer le Safety Shield et les gentleMACS Tubes Selon la norme internationale CEI 62471 ce syst me de lampe a une valeur d exposition limite ELV de 0 91 et d passe le groupe exempt de risques La distance de s curit DS pour le groupe exempt de risques est de 61 cm La distance de s curit pour le groupe de risques 1 est de 20 cm Le risque li au spectateur d pend de la fa on dont est install e et utilis e l appareil La radiation optique mise de ces DELs peut tre nuisible aux yeux une proche distance de vue si la Trough est enlev e D branchez l appareil avant d enlever la Trough Si la Trough est enlev e ne pas regarder dans l clairage des DELs une distance inf rieure 20 cm De plus le lecteur de code 2D utilise des DELs pour illuminer la zone de lecture Ne fixez pas directement le lecteur de code 2D des yeux une courte distance lorsqu il fonctionne Ne d montez modifiez ou enlevez aucune des sources de radiation optique Une radiation optique d appareils d mont s peut tre nuisible aux yeux Surface chaude dangereuse La surface des parties chauffantes du gentleMACS peut provoquer des br lures en cas de contact Ne touchez pas la surface chauffante pendant le fonctionnement de l appareil Niveaux de bruits dangereux Si l a
99. ty reasonable wear and tear negligent or incorrect handling servicing or maintenance non adherence to the operating instructions unauthorized modification of or to any part of this product or use of inappropriate accessories or work materials Miltenyi Biotec s warranty does not cover products sold AS IS or WITH ALL FAULTS or which had its serial number defaced altered or removed or any consumables or parts identified as being supplied by a third party Miltenyi Biotec must be informed immediately if a claim is made under such warranty If a material or manufacturing defect occurs within the warranty period Miltenyi Biotec will take the appropriate steps to restore the full usability of your product Limitation on damages Miltenyi Biotec shall not be liable for any incidental or consequential damages for breach of any express or implied warranty or condition on this product Some states or jurisdictions do not allow the exclusion or limitation of incidental or consequential damages so the above limitations or exclusions may not apply to you This warranty statement gives you specific legal rights and you may have other rights which may vary from county to country or jurisdiction to jurisdiction 99 100 Miltenyi Biotec Germany Austria Switzerland Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 0 Fax 49 2204 85197 macs miltenyibiotec de USA Canada Miltenyi B
100. ufgestellt werden Verst rker eingeschlossen Sch tzen Sie das Ger t vor direkter Sonneneinstrahlung ffnungen und Schlitze am Ger t sind zur Bel ftung gedacht und sollten niemals bedeckt oder blockiert werden da sie das Ger t vor berhitzung sch tzen und einen sicheren Betrieb erm glichen Versuchen Sie nicht das Ger t in eine Einbaukonstruktion zu integrieren oder auf begrenztem Raum unterzubringen es sei denn es wurde eine spezielle Vorrichtung entwickelt und f r ausreichend Bel ftung gesorgt Befolgen Sie die Montageanleitung f r den Aufbau des Ger tes Stellen Sie sicher dass der Hauptstromschalter ebenso wie der Netzstecker f r das Stromkabel leicht zug nglich sind und sich in m glichst unmittelbarer N he zum Bediener des Ger tes befinden Sollte es erforderlich sein die Stromzufuhr zu unterbrechen ziehen Sie den Netzstecker aus der Steckdose Das Ger t ist mit einem Schutzkontaktstecker und einer dreiadrigen Anschlussleitung ausgestattet Dieser Stecker passt nur in eine Steckdose mit Schutzkontakt Das ist eine Sicherheitsvorrichtung Versuchen Sie nicht den Stecker in eine Steckdose ohne Schutzkontakt einzuf hren Wenn Sie den Stecker nicht in die Steckdose einf hren k nnen bitten Sie einen ortsans ssigen Elektriker diese zu ersetzen Das Ger t sollte nur von einer Stromquelle aus betrieben werden die den elektrischen Angaben auf dem Typschild entspricht Sollten Sie Fragen zur Art der Stromversorgung
101. usses und der berhitzung Ein Stromschlag kann zu schweren Verletzungen und bis zum Tod f hren Ein elektrischer Kurzschluss sowie berhitzung kann eine Brandursache darstellen Elektrische Ger te k nnen Funken schlagen welche leichtentflammbare D mpfe oder Materialien entz nden und somit zu Feuer und Explosionen f hren k nnen Benutzen Sie das Ger t nicht in ausgewiesenen Gefahrenzonen z B in sauerstoffangereicherten Umgebungen Das Geh use des Ger tes dient dazu das Risiko eines elektrischen Stromschlags Kurzschlusses und Feuers zu reduzieren Entfernen oder durchdringen Sie keine Abdeckungen mit Ausnahme des Troughs und des Safety Shields Jegliche andere Abdeckung darf nur von autorisiertem Personal entfernt werden F hren Sie nie einen Fremdk rper durch eine ffnung in das Ger t ein Benutzen Sie das Ger t nicht wenn es offen oder zerlegt ist es fallengelassen wurde oder besch digt ist Teile besch digt oder defekt sind die Netzanschlussleitung besch digt ist ein Gegenstand in die Bel ftungsschlitze eingedrungen ist ein Fremdk rper in des Ger t gefallen ist Wenn Flammen oder Rauch auftreten schalten Sie das Ger t unverz glich ab ziehen Sie den Netzstecker und kontaktieren Sie einen autorisierten Miltenyi Biotec Servicedienstleister oder den Miltenyi Biotec Technical Support Das Ger t ist ausschlie lich f r den Betrieb in Innenr umen ausgelegt Sollten Fl ssigkeiten in das Ger t gelangen kann
102. vicios autorizado de Miltenyi Biotec lleva a cabo todas las comprobaciones de seguridad como requerido por el procedimiento de reparaci n para asegurarse de que el instrumento est funcionando correctamente 31 IT FR Es DE EN INFORMACI N IMPORTANTE DE SEGURIDAD 32 Utilice solo opciones y actualizaciones recomendadas por Miltenyi Biotec Consulte con su representante local de Miltenyi Biotec sobre el amplio servicio del instrumento o dir jase a www miltenyibiotec com support El instrumento debe ser transportado cuidadosamente en embalaje especificado por Miltenyi Biotec Si el instrumento est sujeto a una vibraci n excesiva o ca da pueden ocurrir da os internos Si el instrumento necesita ser enviado de vuelta al fabricante para su servicio descontamine el instrumento para eliminar cualquier material peligroso antes del transporte Si tiene preguntas respecto a una correcto descontaminizaci n o transporte contacte al Servicio T cnico Miltenyi Biotec Informaci n sobre la Directiva de Residuos de Aparatos El ctricos y Electr nicos Waste of Electrical and Electronic Equipment WEEE Por favor al final de la vida til de su equipo de Miltenyi Biotec disponga del mismo de acuerdo a la ley WEEE aplicable la cual puede ser diferente seg n el pa s o regi n Los equipos el ctricos y electr nicos pueden contener sustancias peligrosas que pueden tener graves efectos perjudiciales sobre el medio ambiente y o la
103. vous que le prestataire de services utilise uniquement des pi ces authentiques de Miltenyi Biotec ou des pieces de tiers sp cifi es et recommand es par Miltenyi Biotec L utilisation de pi ces non autoris es peut entra ner un dysfonctionnement de l appareil et 39 IT per ES DE EN INFORMATIONS DE S CURIT IMPORTANTES 40 impacter les r sultats Miltenyi Biotec n accorde aucune garantie ou ne prend aucune responsabilit pour des d fauts de l appareil ou des d t riorations r sultant de l utilisation de pi ces inappropri es Apr s l ach vement de tout travail de maintenance et de r paration assurez vous que votre prestataire de services Miltenyi Biotec agr entreprenne tous les contr les de s curit comme le demande la proc dure de r paration pour garantir un fonctionnement normal de l appareil Utilisez uniquement des options et extensions recommand es par Miltenyi Biotec Renseignez vous aupr s de votre repr sentant local Miltenyi Biotec sur les arrangements extensives de support et de maintenance de l appareil ou bien r f rez vous www miltenyibiotec com support L appareil doit tre transport avec soin dans des emballages sp cifi s par Miltenyi Biotec Une d t rioration int rieure peut se produire si l appareil fait l objet de Vibrations excessives ou d une chute Si l appareil doit tre exp di de retour au fabricant pour la maintenance d contaminez l appareil pour liminer tout
104. xigences r glementaires Selon le type et l utilisation de votre quipement des exigences suppl mentaires peuvent s appliquer Pour plus d informations ou si vous souhaitez recycler votre quipement Miltenyi Biotec en fin de vie contactez votre interlocuteur Miltenyi Biotec ou notre support technique CAUTION Il gentleMACS Octo Dissociator with Heaters stato realizzato per garantire un uso sicuro se installato correttamente impiegato da personale specializzato e secondo la normativa generale di sicurezza e le istruzioni d impiego contenute in questo manuale Le istruzioni in questa sezione spiegano i potenziali rischi associati all uso di questo strumento e provvedono a fornire delle informazioni di sicurezza aggiuntive per minimizzare il rischio Si seguano le istruzioni attentamente per proteggere Voi stessi gli altri e l attrezzatura da potenziali pericoli e creare un ambiente di lavoro sicuro Si utilizzi questo strumento solo come specificato dal produttore per evitare danni al prodotto o lesioni al personale Si seguano sempre le indicazioni di sicurezza specifiche del luogo di lavoro e le disposizioni del laboratorio nonch gli standard di salute sicurezza e prevenzione incidenti Si contatti l autorit locale per il controllo delle forniture elettriche le costruzioni della struttura la manutenzione e la sicurezza per ottenere maggiori informazioni in merito all installazione sicura e l impiego dello strum

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