User`s Manual
Contents
1. AL eo okolab H401 T DUAL BL and H401 T SINGLE BL Temperature Controller User s Manual Vers 04 14 This page was left blank 2 1 Revision table Edited pe Giulio Pasquariello Edited with new user manual format July 2014 Amelia Liccardo Chamber connections paragraph ae 03 December 2014 Amelia Liccardo Removal Silvia Foppiano 04 Review December 2014 Amelia Liccardo Index 1 REVISION WER koooeiaae wie ie a eae 3 2 PRE CE EE 5 3 SYMBOLS DESCRIPTIONS rii n E AEN NE E E A 6 PY NOIES E 7 5S EQUIPMENT ZEIEN 8 6 H401 T DUAL AND HAOL T SINGLE DESCRIRTION kk REENEN 9 7 CONNECTIONS AND SYSTEM SEIUbR eessen 9 7 1 Thermal plate CON MCC LON EE 9 7 2 Serial Communication Cable e ein e A EE 12 8 UNIT OPERATION THROUGH TOUCH SCREEN INTERFACE 0000 oe eecsseeeeeeeeeeeeeeeeeeees 13 8 1 POMS ACS E 13 8 2 LED Colours Meaning WEE 14 8 3 Se CI SSS EE 14 Got IDEVICS were lo Leg e RE 14 Snowe SOPONNA OS E 15 onc ee Ma Keen g i igibe 105 e E 16 ede Pale I CIN So ee EE EE EEN 17 8 4 Enter gg e ue 18 54 1 Output Temperature RAMP RATE geegent gebt Seege ee eege beggen 19 GAZ THUIMIE ee e le EE 19 Sa ee H let eler EE 20 CA PENE Or le EE 22 8 4 5 Self Calibration Advanced SettingS EE 23 8 5 EE 24 8 6 WTO PAGS E 24 9 ERROR RUE tege geesde gege egegeeeE A EEEN OANE eebieet 25 10 TEMPERATURE CONTROL MECHANISM WITH OFFSETS 000 0 ec eessessssssesssessnneees 26 11 SUPPOR eege 28 11 1 Web conference for AS2. 2 37 0 Free sensor C 36 7 Accuracy C Figure 22 Advanced Self Calibration settings 8 5 Status Page Press the magnifying glass button to access the Status Page On this page you can see the Status of the Temperature Controller and check that the system is working properly The first two rows duplicate the information from the homepage in the Temperature Tab The lower part of the screen displays the Fine Gauge Thermocouple Free Sensor reading The additional parameters are displayed mainly for technical control reasons In case you requested support from one of Okolab engineers you may be asked for some of these data Device 1 Device 7 a 37 0 37 0 Alarm 4 ty Offset C 0 00 M 0 00 M Relay O DO 15 0 gt 0 169 0 237 Free Sensor C E3 Board Temp C Figure 23 Example of the status page when Fine Gauge thermocouple is disconnected In H401 T SINGLE BL there is only the Device 1 column 8 6 Info Page Press the button with the Okolab logo to access the Info Page This page shows all the information related to the T Unit version Please have this information handy when contacting Okolab for support 24 System information Product Code H401 T DUAL Release date 24 06 2013 Service date 24 06 2013 Serial number 1000 Version 4 36 0 Touch version 5 5 0 32 AD Version 3 2 01 System uptime 19 42 35 www oko lab com Figure 24 Info page showing also the total system up ti
3. 0 C while the Fine Gauge Thermocouple measures 36 8 OC NENNEN REENEN NNN NEEN AN 23 Figure 22 Advanced Self Calibration settings cccceece cece cece cere e reer eee e neers eee e esses eee eee e eee ee eens 24 Figure 23 Example of the status page when Fine Gauge thermocouple is disconnected ccceee eens 24 Figure 24 Info page showing also the total system up Dme cece eee eee e sees sees eee eeeeeeeeeeeee 25 Figure 25 Safe mode page ee eee eessceceeee eee e een e nee nsseceeeeeeeeeeen anne essceeeeeneeeeetnnanansaeeeeeeseneentnnees 25 Figure 26 COMIMUNICatiOn Error page erriren ninnaa n iin iA T E A EEEE A a Enea 26 Figure 27 Free Sensor attached on the internal base of the Petri Dish sssssusssnsunsnnsnnennrnrrnnenrrnrennns 27 31 WARRANTY Okolab S r l warrants the H401 T DUAL to be free of defects in materials and workmanship for a period of two 2 years starting from invoice date If the unit malfunctions it must be returned to the factory for evaluation If the equipment has to be returned to the factory please ensure that is carefully and properly packed Okolab S r l accepts no responsibility for damage due to unsatisfactory packing Upon examination of Okolab S r l if the unit is found to be defective it will be repaired or replaced at no charge This warranty does not apply to defects resulting from any actions of the purchaser Components which wear are not warranted Okolab S r l neither a
4. to connect the Serial Communication cable to A your PC you have to install a USB to serial converter following the installation instructions of the manufacturer If you want also to log you have to use the COM port number that Windows has assigned to the drive 8 Unit Operation Through Touch Screen Interface 8 1 Homepage Temperature set point temperature value O 3 0 Ipae Del Plate SM U Device 1 Max temperature reached Device 1 Status Led ithi i i evice atus Le FID within the time period Device 2 Name CH j 0 C Device 2 Name _ a 36 8 Device 1 minimum temperature reached within the time period Dev 2 j Device 2 Status Led late SM U 87 D Device 2 Max temperature reached within the time period s 6 8 gt Device 2 Current Device 2 minimum temperature temperature value reached within the time period Touch here to see details on unit statu Touch here to access system Touch here at any time to come back to the Main page options and settings Figure 5 Overview of the homepage screen The Temperature tab is located on the top of the screen It displays the temperature information relative to each connected heating device The first line on the display shows the temperature set point which must be the same for both devices connected Below it are displayed the current temperatures for Device 1 Dev 1 and Device 2 Dev 2 To the left of Dev 1 and Dev 2 a status indicator is also displayed green dot in t
5. EN EEN ENNEN NEEN EEN EEN 13 Figure 6 a how to enter settings page and b settings page cece cece cece sees eee eeeeeeeeeeeeeeeee 15 Figure 7 a configuration page and b device settings page cece cece eee e esses eee sees eee eeeeeeeeeeeeeee 15 FOUTE 35 e e ef CaO LEE 16 Foure 9 le lat ug d ue EE 16 Figure 10 a how to enter settings page and b settings Dade E 17 Figure 11 How to choose the touch screen Orientation ccc cece cece cere essere esses eee eeeeeeeeeeeeeeeeeee 17 Figure 12 a how to reach alarm page and b alarms settinge E 18 Figure 13 a how to enter settings page and b settings page 18 Figure 14 a how to configuration page b how to enter ramp rate page and c ramp rate settings 19 Figure 15 a how to enter calibration page from settings page and b calibration page cc ccceeee eens 20 Figure 16 Example of a petri dish with Fine Gauge Thermocouple placed onto a Thermal Plate 20 Figure 17 How to start self calibration procedure click on start and then OK ccecceee cece cess eeeeee eee eees 21 Figure 18 Examples of Self calibration procedure NNN 21 Figure 19 Example of a successful self calibration procedure ss ssesssrssrsnrrnrrnrrnrrnnrnnrnrrnrrnnrnnrnnennne 22 Figure 20 How to reset the offset stored after a calibration procedure sss ssssssssrssrrssresnrrsnrenrrnsrenns 22 Figure 21 Example of a manual calibration procedure the thermal plate sensor reads 37
6. SISTANCE ANC Craining teeter EEE E EERE EERE EERE REESE EEE REESE EE EES 28 La WCC e NEE 28 KSC EE nuer SOC CII eg Ten E 29 12 TROUBLESHOOTING teen anette neta tle h anette eee teeta lente eee tee eee te eee eet en tere ee ee eee te ee eee ee eee 30 13 PIGORE TABLE E 31 The H401 T DUAL BL is a dual channel temperature controller allowing temperature set up and control of two separate heating devices It is a compact and effective solution to reliably maintain the desired temperature for living cell cultures right on the microscope stage within the range 25 50 C The system comprises the Temperature Controller and two heating devices The H401 T SINGLE BL is a touch screen digital temperature controller allowing temperature set up and control of a single heating device A variety of heating devices can be connected to the Temperature Controllers for example heating plates both glass and metal for microscope stages glass heating tables metal heating pads or objective heaters The Heating Plates both glass and metal are designed to fit into the opening of XY stages of upright and inverted microscopes Several models of glass and metal heating plate inserts can be selected from the Okolab portfolio Custom solutions are available upon request The Glass Heating Table can be placed in the optical path of any stereomicroscope It provides a large flat clear warm surface where one or more specimens can be maintained at physiological temperat
7. T SINGLE BL with two or one Okolab devices H401 T DUAL BL 24 VDC Power Feeder Figure 2 Thermal plates connections 8 Connect the power adapter to the Temperature Controller Tip gt Okolab provides the correct power adapter compatible with the devices configuration selected by the user The majority of configurations use a power adapter with Rating Power of 60 W The following configurations REQUIRE a power adapter with Rating Power of 120 W two H401 T PAD two H401 Glass Table or one H401 Glass Table and one H401 T PAD 10 Please use the power adapter provided by Okolab or a power adapter for the recommended wattage to prevent system instability random reset unexpected shutdown or problems due to insufficient power If you start with a single device and you add a second one at a later time please ask an Okolab representative for the correct power adapter compatible with the new configuration In no event shall Okolab S r l be liable for any direct incidental or consequential damages of any nature or financial loss resulting from using the incorrect power adapter 9 If you have a single heating device connect it to Device 1 port If you have two heating devices connect one to Device 1 and the other to Device 2 There is no Device 2 port on the rear panel of H401 T SINGLE BL 10 Turn Temperature Controller on using the rocking switch located on the right side panel The following message
8. ber of degrees Celsius and for a specific amount of time See Figure 13 to access the Alarm Settings 17 Time Min j Touch Screen Alarms Dev C 2 Buzzer Calibration Configuration Cancel Soe SOCE BOCE Figure 12 a how to reach alarm page and b alarms settings Wait until the system has reached steady state The example in Figure 13 shows a temperature set point of 37 C and a Temperature Deviation of 0 5 C for a duration of 10 minutes This means that an alarm will be triggered if the temperature reaches a value equal or less than 36 5 C or equal or more than 37 5 C for a period equal or longer than 10 minutes Note k If you want the Alarm to be acoustical rather than just being displayed then flag Buzzer 8 4 Temperature Settings Temperature options are accessed by clicking the Settings icon look at the touching finger Tec 37 0 Touch Screen Alarms t Calibration Configuration Figure 13 a how to enter settings page and b settings page 18 8 4 1 Output Temperature Ramp Rate In some applications i e in heating microscope objective body and lens users may want to change the heating rate The Temperature Controller allows you to modify the heating rate of each device independently see Figure 14 LZ 2 Le SCENE Touch Screen Alarms Devices Ramp rate gt Cfmin Calibration Figure 14 a how to confi
9. g device you may have to modify the offset and re calibrate for better accuracy Offset values should be evaluated keeping in mind that the Specimen temperature reading error is 0 2 C This procedure can be performed for both devices 27 Okolab staff offers excellent customer support To contact one of our engineers please see below 11 1 Web conference for assistance and training Together with your equipment you received a web cam and headset You can request remote Support over the web Please follow these guidelines to facilitate setting up your web support Webcam installation instruction and Cd rom included Last Skype installed www skype com Register yourself on www skype com to have an account Set the audio and video and test them using Skype software Contact our technical support sibillo oko lab com by e mail to take an appointment for the web assistance 11 2 Technical Support Please do not hesitate to contact Okolab should you need any further commercial information or technical support Please check Okolab web site www oko lab com for news events new products and general FAQ For COMMERCIAL SUPPORT lanzaro oko lab com Phone 39 081 806 2624 Fax 39 081 876 4410 Mobile 39 348 96807 17 For HARDWARE SUPPORT sibillo oko lab com Phone 39 081 806 3470 Fax 39 081 876 4410 Mobile 39 348 96807 18 Okolab S r l Via A Olivetti 1 80078 Pozzuoli NA Italy 28 11 3 Tec
10. guration page b how to enter ramp rate page and c ramp rate settings In the Ramp Rate page of the configuration screen you can modify the heating rate of each device In the example shown in Figure 14c we have a thermal plate connected as Device 1 and an objective heater band connected as Device 2 In this configuration Device 1 was set at the max heating rate while Device 2 is set to be heated at a rate of 0 5 C per minute For H401 T SINGLE BL there is only Devicel Ramp rate 8 4 2 T Unit Calibration The Temperature Controller is factory calibrated at a room temperature of 23 C If your laboratory room temperature differs by more than 1 C from 23 C please consider performing an on site Calibration Procedure for better accuracy The procedure can be performed both on Device 1 and on Device 2 but only on one device at time since it requires the use of the Fine Gauge Thermocouple The following pages show the procedure in detail for Device 1 with a T Glass Plate connected It can be performed with all other compatible devices following the same steps 19 Calibration Device 1 Plate SMU kl OG Gem we TC 37 0 Touch Screen Alarms Free sensor SC 36 7 Offset C 0 00 M Accuracy C dEr Calibration Configuration Figure 15 a how to enter calibration page from settings page and b calibration page There are two different calibration modalities Manual and Self Calibration Select which one
11. his example The minimum bottom and maximum top temperature values that were reached within a selected time period are displayed on the right of the current temperature To see the temperature 13 measured by the Fine Gauge Thermocouple click on the Status button with magnifying glass on the bottom of the Touch Screen For H401 T SINGLE BL there are only the Device 1 variables Tip gt The Touch Screen is pre set with a set point Temperature of 37 C Once turned on it will start operating to reach this set point value 8 2 LED Colours Meaning you ve set and that the system is working properly actions on your part are required Please note the Yellow light will also appear every time you A GREEN indicator means that set point value has been reached within the tolerance A YELLOW indicator means that the system is working towards reaching set points NO change the Temperature set point An ORANGE indicator means that the current gas concentration is not correct and its value is out of the set tolerance see section ALARMS in paragraph 8 3 4 While giving time to the system to recover from this out of range value please double check if there may be reasons not depending on the unit for which it is not properly working Verify if all cables are correctly connected few hours the Red light doesn t turn to Green turn off the system and then turn it on again If A RED indicator means that some
12. hnical Specifications H401 T DUAL BL and H401 T SINGLE BL Technical Specifications Range 25 50 C Temperature Device1 2 Step size 0 1 C Accuracy 0 2 Operating Temperature 0 C 55 C 29 12 Troubleshooting Incorrect operations are often mistaken for malfunction If you think that there is something wrong with a component see the troubleshooting scheme below If the problem persists even after troubleshooting as described below please contact Okolab for support Symptom Probable cause Remedy P nnect the cables to the Input Output wrong connections dee S Se Device off input and to the output Actual temperature far from the Set Properly set the system Alarm rings Point temperature Cable disconnected Connect the cable ERR_1 Temperature Sensor Contact Okolab to receive assistance damaged No temperature displayed ERR_ E T ERR A Error on thermocouple Check the Thermocouple position ERR_6 Temperature read by Thermal plate sensor too high or too Contact Okolab to receive assistance low ERR_7 Generic Error Contact Okolab to receive assistance I check the previous troubleshooting but I cannot Contact Okolab to receive assistance solve the problem 30 13 Figure Table Figure 1 o RE EE Te E 9 Figure 2 Thermal plates connections EE 10 Figure 3 Initialization screechot E 11 Figure 4 Serial Cable connection NN Error Bookmark not defined Figure 5 Overview of the homepage screen NE
13. is fully immersed in liquid Place the Reference Sample so prepared on the heating device and turn the Temperature Controller on 3 Wait until steady state is reached By measuring the specimen temperature with an external sensor the offset values can be adjusted as described below 26 A Figure 27 Free Sensor attached on the internal base of the Petri Dish Place the Petri Dish onto the device Tip Please monitor the room temperature during the calibration and reduce air drafts to reduce the forced convection If possible avoid performing this procedure directly under AC vents 5 10 11 From the Home page press the Settings Icon and then follow the instructions of the paragraph 8 4 4 to access the Manual Calibration Page Manually adjust the Reading Offset to have the specimen reach the set point temperature Once you accessed Manual Calibration Page press or to increase or decrease the Reading Offset of your device Press Save to confirm the new Reading Offset of device Press Home Page icon to go back to the Home Page Wait until the steady state of the Fine Gauge Thermocouple is reached Reduce the Reading Offset of device if the temperature of the Fine Gauge Thermocouple at steady state is lower than Temperature Set Point Increase the Offset if the temperature of the Fine Gauge Thermocouple at steady state is higher than Temperature Set Point If you change heatin
14. me 9 Error Message If the system graphics fails to load the system reverts to a non graphic display and shows the following screenshot SAFE MODE Set point af 4 I C Dev 1 af 4 I C Dev Z af 4 RESTART IN NORMAL MODE Figure 25 Safe mode page If this occurs press the restart in normal mode button so you can restart the device in normal mode If this persists please contact Okolab for support When the system identifies a communication or electronic problem it may display the following screenshot 25 Error message Comunication Error Please contact Okolab Touch version 5 5 0 32 wWww w oko lab com 7 Figure 26 Communication Error page This error may be due to a communication problem between Touch Screen and Temperature Controller Contact Okolab for support http www oko lab com onlineSupport page Note gt Please note that this screen will always appear in the same orientation If your Temperature Controller is wall mounted it will appear upside down 10 Temperature Control Mechanism With Offsets The temperature of the heating device can be adjusted by manually changing offsets as follows Input temperature set point e g 37 0 C 2 Insert the Fine Gauge Thermocouple Free Sensor into a Reference Sample e g a 35mm dish If needed secure the external sensor to the bottom of the dish with tape Add liquid media or water to the dish Ensure that the tip of the sensor
15. nd Once you have input the new set point temperature press Set to save or Cancel to undo Figure 9 Set point setting 8 3 3 Touch Screen Settings Touch Screen Settings are reached by following the two steps shown below look at the touching finger 16 TLC 37 0 bev 1 Plate SMU 36 4 3 6 8 4 See Touch Screen Alarms bev 2 Plate SMU ti AK Calibration Configuration Figure 10 a how to enter settings page and b settings page 8 3 3 1 Rotate The first time you turn the system on it is important to set the screen orientation S Touch Calibration Rotate A Screen Saver GE S Figure 11 How to choose the touch screen orientation The screen is set by default in its portrait orientation If you press the rotate button you can rotate the screen by 180 A 180 screen rotation is recommended in case you want to wall mount the Temperature Controller In this page you can also disable the screen saver feature by default if the display has not been touched for 20 minutes a black page with only the Temperature Tab is shown Now you can press the Home button to go back to main page Tip gt If you want to wall mount the Temperature Controller contact Okolab to receive further assistance 8 3 4 Alarm Settings You can set an Alarm to be triggered in case the temperature of the heating device deviates from the set point by a specific num
16. product label Avoid excessive induction noise static electricity and magnetic fields Do not expose this instrument to rain or moisture Prevent throttling and kinking of cables Do not start up the equipment if the supply cable is damaged Connect the equipment only to grounded mains power socket Before starting assemble the equipment while unplugged from an outlet Prevent metal fragments or lead wire scraps from falling inside instrument case to avoid electric shock fire or malfunction International caution symbol marks this device IMPORTANT read the Safety Notes before installing using and commissioning this device as A the notes contain important information relating to safety and EMC Not following these instructions can result in damage or breakdown of the device and its accessories We reserve the right to make technical modifications IN NO EVENT OKOLAB S R L SHALL BE LIABLE FOR ANY DIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE OR FINANCIAL LOSS RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT 5 Equipment supplied 1 Control T Unit H401 T DUAL or H401 T SINGLE 2 One or two heating devices chosen by the User Required but not supplied purchased separately 3 Fine Gauge Thermocouple An external temperature sensor used when performing a Self Calibration Routine or to monitor ambient temperature 4 Power adapter and power cord x1 The T unit is powered with 24V DC powe
17. r supply Okolab provides a shielded power adapter compatible with the heating devices selected by the user 5 DATA LOG Software Data Logger to log temperature data from any OKOLAB device Optional 6 H401 T DUAL and H401 T SINGLE Descripti pa w i ce geg rest gue eae an eo ei dg Kemp w nlf a Figure 1 H401 T Dual BL Rear panel 24 VDC Power Input Power switch Serial port Ground connector Fine Gauge Thermocouple port Device 1 port Device 2 port This port is not present in H401 T SINGLE BL N OF OW BR U N Be 7 Connections And System Setup If your application requires controlling the sample temperature for a few hours under the microscope without controlling the composition of CO you can connect one or two heating plates or Glass Tables to the H401 T DUAL BL see paragraph 7 1 Note gt This temperature controller can manage up to two devices at the same time if you need to control the temperature of only one heating device use H401 T SINGLE BL 7 1 Thermal plate connections Okolab heating plates compatible with H401 T DUAL BL and to the H401 T SINGLE BL Glass and Metal Plate H401 T PAD Objective Band Heaters H401 Glass Table For a list of all available models and specs of each product see www oko lab com A Do not turn the device on until you have completed and checked all connections The following instructions will show you how to connect the H401 T DUAL BL or H401
18. rt and then ok Figure 18 shows the progress displayed during Self Calibration Self calibration Self calibration Device 1 Plate SMU Device 1 Plate SMU SE 37 0 dE 37 0 Free sensor C 36 4 Free sensor C 36 7 Offset C 0 00 Offset C 0 18 Progress 15 Progress 66 Figure 18 Examples of Self calibration procedure Wait until the dialog box in Figure 19 appears It is possible to use the touch screen while the calibration is ongoing and reach pages other than the one above Figure 18 21 Once the calibration will be completed the screen in figure 19 will automatically reappear regardless of the screen you will be in Self Calibration Completed successtully Figure 19 Example of a successful self calibration procedure Once the Self Calibration is completed you can remove the Fine Gauge Thermocouple from the Petri dish and start your experiment Note gt On the Self Calibration page you can also reset the offset to the factory value of 0 00 C by pressing the Reset button Calibration Device 1 Plate SML Device 3 d fei 37 0 Free sensor C 36 7 Offset C 0 00 Mi Accuracy C 0 15 Figure 20 How to reset the offset stored after a calibration procedure 8 4 4 Manual Calibration This procedure is alternative to the previous one and operates by adding a fixed offset to the temperature of the plate so that the sample temperature measured by the Fine Gauge thermocouple
19. ssumes responsibility for any omissions or errors nor assumes liability for any damages that result from the use of its products in accordance with information provided by Okolab S r l Okolab S r l warrants only the parts manufactured by it will as specified and free of defects Okolab S r l makes no other warranties or representations of any kind whatsoever express or implied except that of title and all implied warranties including any warranty of merchantability and fitness for a particular purpose are hereby disclaimed LIMITATION OF LIABILITY the total liability of Okolab S r l shall not exceed the purchase price of the component upon which liability is based In NO event shall Okolab S r l be liable for consequential incidental or special damages 32
20. thing may have been damaged inside the unit If after the problem persists please contact Okolab for assistance http oko lab com onlineSupport page 8 3 General Settings 8 3 1 Device configuration The first time you turn the system on it is very important to verify that you are using the correct configuration parameters for your device shows the homepage of a temperature controller with one Heated Glass Plate and one Heated Table If you have different heating devices or if you want to disable one of the outputs you could perform the following procedure 14 TLC 37 0 Touch Screen Alarms k T Calibration conti ijon Figure 6 a how to enter settings page and b settings page Access the Settings Page by pressing the settings icon on the homepage button with gear Next press Configuration Configuration Devices Device 1 Figure 7 a configuration page and b device settings page On the configuration page select Devices In the next screen you can set the device currently connected to Device 1 and Device 2 The system will load the correct parameters for the selected device For H401 T SINGLE BL Only Device 1 is present in the configuration 8 3 2 Set point change To change set point simply touch the Temperature Tab on the homepage 15 Figure 8 Set Point change The setting regulation page will appear Modify the set point by clicking a
21. upplies you with important information to successfully setup and use the instrument 4 Safety Notes In order to achieve maximum performance and to ensure proper operation of your new equipment please read carefully the following safety notes and the instructions If you have any question please contact Okolab The equipment must only be used as intended and as described in this Manual Equipment should only be operated by technically qualified personnel Do not start up the equipment if some of its parts are damaged This instrument is not intended for use in locations subject to flammable or explosive gases Transport the equipment with care Equipment and its internal parts can be damaged by dropping and by shock Some equipment parts may reach temperatures above 40 50 C Take care when touching it This device is not designed for use under medical conditions Avoid rapid changes in ambient temperature which may cause condensation avoid direct air draft from air conditioner exposure to direct sunlight and excessive heat accumulation Do not close Air Intake Do not disassemble any part of the system and in particular of the heating units Do not disconnect cables while in operation Do not use a volatile solvent such as paint thinner to clean the instrument Deformation or discoloration will occur Use a soft dry cloth to remove stains from the instrument Do not exceed voltage indicated in this manual and on the
22. ure It is also suitable for small animal surgery The Metal Heating Pad is a temperature controlled surface that can be positioned on any laboratory workbench or desk surface e g table top cabinet work surface etc to keep Petri dishes Chambered and Glass Slides and Tubes at the right temperature before microscope observation The Objective Band Heater is designed to reliably maintain the desired temperature of the objective body and lens This is important when the objective is in contact with the sample during water dipping observation or the tissue culture dish when imaging with oil immersion The system is controlled via an intuitive user friendly touch screen interface This instrument works with low voltage power and accepts 24 Vdc Temperature stability is 0 1 C Temperature measuring accuracy is 0 2 C Okolab DATA LOG is optional software allowing logging temperature data or modification of selected parameters DATA LOG can be installed on your PC Serial port connection to the Temperature Controller allows data download 3 Symbols descriptions The following symbols identify important information to note CAUTION or WARNING This symbol warns you about the risk of electrical shock CAUTION or WARNING or IMPORTANT This symbol warns you of circumstances or practices that can affect the instrument s functionality and must refer to accompanying documents Tip gt Supplies you with helpful suggestions Note gt S
23. will appear on the Touch Screen of the Temperature Controller fx 4 ee okolab Figure 3 Initialization screeshot 11 The Homepage will appear after completion of initialization The temperature indicator will be initially yellow 11 If any one heating device were to reach max temperature the Temperature Controller will automatically turn off both devices To avoid malfunctions disable disconnect the improperly working device 7 2 Serial Communication cable connection Figure 4 shows serial cable connection to the Serial port located on the right side of the Temperature Controller The Temperature Controller can be connected to a PC through the serial connection allowing collecting and saving the temperature by using DATA LOG Okolab DATA LOG is optional software allowing logging temperature data or modifying selected parameters With DATA LOG installed on your PC you can select variables to monitor over time through a simple and intuitive interface The collected data can then displayed in various graph forms for easier and faster analysis at the end of the experiment The DATA LOG also allows modifying the parameters of the system Temperature set point Offset etc directly from your PC Serial Port Figure 4 Serial Cable connection Note gt Use a standard Serial Communication cable to connect the PC to the Serial Port on the right side of Temperature Controller 12 If you do not have a serial port COM
24. will reach the set point The Temperature Controller will adjust the power output to keep the temperature of the Plate at the desired value taking offset into account Wait for the Fine Gauge Thermocouple to reach a stable value Next press or to input the difference in temperature between the plate and the reading from the Fine Gauge thermocouple in this example 0 2 C then press Save 22 Manual calibration Device 1 Plate SMU H Bei 37 0 Free sensor C 36 8 Offset C Figure 21 Example of a manual calibration procedure the thermal plate sensor reads 37 0 C while the Fine Gauge Thermocouple measures 36 8 C Only one offset can be stored at any one time Hence a Manual Calibration will overwrite the result of any previous calibration including a Self Calibration one 8 4 5 Self Calibration Advanced Settings The Self Calibration Procedure continuously adjusts the temperature of the heating module until the specimen reaches at 37 C Press Advanced Settings see Figure 20 Figure 22 shows how you can increase or decrease the accuracy of the calibration procedure from a minimum of 0 15 to a maximum of 0 3 C A lower value will extend the duration of the calibration procedure but it will result in higher accuracy This procedure is fully automatic and once complete it will permanently store the correction factor for the heating module 23 Advanced settings TAC Dev 1 37 0 TAC Dev
25. you want to perform by pressing the corresponding button see Figure 15b Place the Fine Gauge Thermocouple into a Petri dish Fill the Petri dish with enough water to ensure that the tip of the Fine Gauge Thermocouple is fully immersed 3 4 ml of distilled water Close the Petri dish with its lid and then place it on the Heating Plate see Figure 16 Figure 16 Example of a petri dish with Fine Gauge Thermocouple placed onto a Thermal Plate For H401 T SINGLE BL there are only the Device 1 calibration options 8 4 3 Self Calibration This procedure ensures the highest accuracy on specimen temperature It monitors the temperature measured by the Fine Gauge Thermocouple in a Petri dish placed on the device under calibration and adjusts the temperature of the heating module in order to 20 keep the specimen at 37 C This procedure is fully automatic and once complete it will permanently store the correction factor for the heating module Press the Start button to run the Self Calibration procedure O The Self Calibration procedure it may take 1 2 hours to complete the average calibration time is 90 minutes Calibration Device 1 Plate SMU 37 0 3 Please insert the TE Free sensor C 36 7 free Sensor in Petri Offset C 0 00 M S water Accuracy C 0 15 Self calibration will be performed with a setpoint of 37 0 C 5 Figure 17 How to start self calibration procedure click on sta
Download Pdf Manuals
Related Search