Micro10x - Hudson Robotics
Contents
1. Positive values will move the dispensing location above the travel height Entering O will keep the travel height and the dispense height the same Height This is the height the tips will travel above the nest during the dispensing This value should be at least 1mm above the measured plate height The Standard Microtiter plate is 13mm Wells Select from 96 384 or 1536 11 Orientation Select between the Portrait or Landscape plate orientation This is determined by which manifold you are working with 12 channel will run in landscape while the 8 channel runs in portrait configuration Tip Touch Activate or deactivate tip touching Tip Touch Adjust where in the plate well you would like the tips to touch after the dispensing Depending on the plate type you will center your tips in row A of either a 96 or 384 well plate Once the tip is centered you will adjust the Y axis either to the front or rear of the well until the tip is touching the plates well A good location for the height of the tip is Imm below the top of the Plate Figure 22 KE wt 2 A A W AR Ne A Va Ae e E Der Ne dre Figure 2 3 2 Fill Pattern Select the rows you would like to fill the default 1s all wells 2 4 Prime Submenu The MC10X can store up to 10 prime programs Fields are adjusted in the same manner as the Dispense programs Figure 2 4 1 12 Prime Program 01 Figure 2 4 1 Volume This is the total vol2. 7 Settings Submenu There are critical settings that can be adjusted by an administrator Figure 2 7 1 When you access this submenu you will be ask a question please click yes to continue to the Settings menu Figure 2 7 2 15 Dispenser Settings Adjust Prime Position EDD Adjust Track Nest Height Adjust Plate Origin Row Column Spacing Tips in Manifold Enable Disable Pump Figure 2 7 1 Altering settings may cause undesired operation Continue Auto Prime Figure 2 7 2 16 Adjust Prime Position This allows the user to jog the dispensing manifold to the position where they would like all priming functions to occur When you click on the button you will be asked 1f you want to move to the location If you are unaware of where the location is click no and work from the home position Figure 2 7 3 After making an adjustment in any of the fields press OK To lose the changes press Cancel Figure 2 7 4 The tips should sit 1 4 into the trough and be closer to the front of the trough so you do not kink the tubing coming from the pump fitting Figure 2 7 5 current location Figure 2 7 3 17 Calibration X Position Y Position z Position Figure 2 7 4 Figure 2 7 5 Adjust Track Nest Height This value should be evaluated and adjusted if manifold types or plate nests are changed This will set the 0 position for the Z axis All dispense pro
3. L Speed 30 Depth O mm Height 15 mm Wells 96 Orientation Landscape Perform Tip Touch Change Tip Touch Change Fill Pattern Figure 2 8 1 21 Section 3 Maintenance of the Mirco10X Fluid Path 3 1 Removal of the Pump Head Remove the 4 mounting screws from the face of the pump Figure 3 1 1 Support the pump with your hand to prevent it from falling from the mounting plate 4 mounting screws for Pump head Figure 3 1 1 Gently pull the pump diagonally away from the mounting plate Care must be taken not to pull the piston more than 1 out of the cylinder damage to the piston seals may occur Figure 3 1 2 Once the pump head is far enough away from the pump module you will slowly lift up on the entire pump assembly You will need to remove the piston drive shaft from the pivot bearing inside the pump module Figure 3 1 3 22 e 4 a in _ gr VS oo Figure 3 1 2 Figure 3 1 3 23 3 2 Disassembly of the Pump Head Module After the pump head module is removed from the pump you will able to access the Teflon adapters The adapters lock the liner and piston into the proper position inside the cylinder case You will remove the adapters by using a 5 8 open ended wrench and turning the adapters counter clockwise until they are removed from the cylinder case Figure 3 2 1 When the adapters are removed you will notice that there are two flat surfaces mach
4. Microl0x User Manual Rev 2 1c Hudson ROBOTICS INC 10 Stern Avenue Springfield NJ 07081 Tel 973 376 7400 Fax 973 376 8265 Fottoen ee 3 Section Le SUD EE 4 lee 4 Te 2 IP PAIS N S dedans de Rec et ee cn idee cn 6 AS Erte mal Cables a nets nee linda 7 Section 2 Operation of Micro10X through the Touch Screen Controller 9 ZE ORTO P TER as On ee Or Rte en 9 2 DISpense SUUDME E 10 EMS SU DIME OU naaa 12 2 Empty Dis Pen Se tai LS di dl caes 13 DO AUTO PIME EA IA dr 14 ZE SC Unas UDINE A ni en ee term enr moine 15 2 5 plating and Stopping LLOC ant RNA 20 Section 3 Maintenance of the Mirco10X Fluid Path ssssssssssssssesssee 22 SERA WE 22 3 2 Disassembly of the Pump Head Module ss 24 3 3 Cleaning of the Pump ASS MD Vaciar id 26 3A Pump module COMPORTER AUS LME MAN EE 26 Se Addinonak Pare EE 2 Section 4 Micro10x Valid ation cccccccssssssssssssssssssssssssssssssssssssssssssssecssees 28 Section 4 1 General Validation After Cleaning cc ceeccccccccceceeeeeseeeeeeeeeeeeneeeeees 28 Section 5 Hardware Specification ccccccccccccccccssssssssssssssssssssssssscccccccssssseees 29 Secondo ls General E 29 GEHEIT 29 AA O A de ne Do co 30 Ao A e 30 Section E TT latinas 30 SECO O Environmental etica 30 Introduction The Hudson Robotic Microl0x is a fast compact accurate benchtop instrument for reliable automated bulk reagent dispensing i
5. ear actuator 0 006mm resolution approximately 1 2 second for entire travel distance Stainless steel covering anodized aluminum frame Stainless steel casting Ceramic cylinder liner and Ceramic piston 29 e Liner Bore Size Va e Tubing Material Peek fittings on Telfon tubing e Max Shot Size 225ul per revolution e Min Shot Size 12ul per revolution e Max Flow 0 225 lps 60 Max speed Section 5 3 Manifold e Fixed Tip Manifold Material Peek e Fixed tip Material Stainless Steel Section 5 4 Dimensions e 11 HX7 W X 10 D Does not include Nest plate e 11 HX7 W X 15 D Includes Nest Plate e Weight I5LBS Section 5 5 Electrical e Power Input 24VDC 4A e Fuses Internal T4 0 A 250 V e Grounding Through the power cord must be properly earth grounded e Computer Interface RS 232 straight through serial cable Section 5 6 Environmental e Operating Temperature 15 to 40 C 59 to 104 F e Operating Humidity 0 to 85 no condensation e Storage Temperature 20 to 65 C 4 to 149 F e Altitude Up to 2000m 30
6. ed on The first cable is the pump cable this is a black coiled cable that has two 90 connectors The orientation of the cable does not matter when plugging the cable in The pump cable is plugged into the rear of the pump on one side and the other side plugs into the right side connector on the rear of the MC10X Figure 1 4 1 The second cable is the controller cable this is the black coiled cable that is attached to the controller this cable will get plugged into the left side connector on the rear of the MCIOX Figure 1 4 1 The last cable is the 24VDC power cable that will get plugged into the 4 position circular receptacle located on the right side rear panel of the MC10X Figure 1 4 1 D E T Taar fiie y f rr Le y CE GE WSA Pump Cable Power Switch Serial Port Power cord Port for optional DC Inlet for 4 way valve Pump Cable Controller Cable Figure 1 4 1 Section 2 Operation of Micro10X through the Touch Screen Controller 2 1 Controller Features The Microl0X comes with a windows based touch screen to control the unit On the top cover of the Microl0X there are two locations to slide the controller into Figure 2 1 1 So 1t doesn t matter which way the unit 1s facing because you will able to access the control panel from either orientation Figure 2 1 2 If either orientation 1s not accessible then you can keep the controller separate from the MC10X and leave
7. grams will reference this point to determine absolute dispense height The manifold should be positioned as close to the surface of the 18 plate nest without touching it To adjust place a sheet of paper or business card on the plate nest Jog the manifold down until it touches the paper but not pinch it to the nest Figure 2 7 6 aa d Figure 2 7 6 Adjust Plate Origin This value should be evaluated and adjusted 1f manifold types or plate nests are changed This 1s the reference point for all well positions in a SBS standard plate This 1s taught by aligning tip 1 on the manifold with the intersection of wells Al A2 B1 and B2 in a 384 well plate Tips 2 12 should align with every other well intersection in that row Figure 2 7 7 19 Figure 2 7 7 Adjust Row Column Spacing This setting 1s set by the manufacturer and should not be adjusted Of Tips in Manifold This setting is set by the manufacturer and should not be adjusted 2 8 Starting and Stopping a Program Press the Next or Prev key on the TouchScreen to select the program number you would like to run When the desired program 1s selected press the RUN button on the TouchScreen Figure 2 8 1 To stop a program press the CANCEL button on the TouchScreen The dispenser will pause you may then choose to abort or continue the dispense program To abort the program press YES to complete the program press NO 20 Dispense Program 01 Volume 100 u
8. ined in the liner that need to be re aligned at the correct location before the adapters are re installed Figure 3 2 2 Now remove the pump bracket from the pump cylinder by removing the flathead screw using a 1 8 allen wrench Figure 3 2 3 This will allow access to the seal nut Using an adjustable wrench remove the seal nut by turning the nut counter clock wise Figure 3 2 4 Figure 3 2 1 Flat Spot on Liner Figure a 24 Figure 3 2 3 Figure 3 2 4 With the Seal nut loose you will carefully slide the piston seals and seal nut out of the cylinder case Figure 3 2 5 Make sure that the seals do not come off the piston if you force the seals back on you may damage the surface of the seal that sits against the piston If this happens you will have to replace the seal with a new one 29 Figure 3 2 5 3 3 Cleaning of the Pump Assembly All of the pump parts may be cleaned in an ultrasonic cleaner with a dilute detergent solution The parts must be thoroughly rinsed prior to autoclaving 3 4 Pump module Components 26 toto PMPO Cylinder Nut Gland Washer o Teflon Lip Seal 4 Rulon RA PMP 1017 6 2 MCFI 18202 00 Adapter Teflon 3 5 Additional Spare parts MCF1 24000 00 Tubing Pump to Manifold set MCF 1 25000 00 Tubing Pump to Reservoir set MEC 1543 Dispense manifold 8 channel MEC 1544 Dispense manifold 12 channel 27 Section 4 Micro10x Validatio
9. it near the unit on the lab bench Hudson a Hudson Figure 2 1 1 Figure 2 1 2A amp B 2 2 Main Menu When you power the MC10X up it will automatically boot and start the MCIOX program on the controller The unit will also home all the axes when the unit is powered up After initializing successfully the controller will display the main menu screen From there you can access the following sub menus Dispense Prime Empty Dispenser Auto Prime Change Settings Figure 2 2 1 Figure 2 2 1 2 3 Dispense Submenu The MC10X can support up to 20 dispense programs When you first enter the dispense submenu you will be at dispense program 1 Press the Next or Prev key Fig 2 2 1 on the Touch Panel to select the program number you would like to setup Any setting for the dispense program can be modified by clicking on its menu bar Doing this will put you into the settings screen for that particular value 10 Dispense Program 01 Height 15 mm SH Wells 96 Change Fill Pattern L lt lt prev Next gt gt Run Figure 2 3 1 Volume Any integer value from 1 to 9999uL may be selected Speed This adjusts the percentage of the pumps maximum speed More viscous liquids will require a slower pump speed to avoid cavitation Depth Negative values are the distance below the travel height where the tips will move during the dispensing allowing the user to dispense with the tips in the wells
10. n Section 4 1 General Validation After Cleaning To Ensure that the unit is dispensing properly after cleaning you will need to perform a validation dispense To perform a validation dispense you will need the following items e Clear 96 well Polypropylene Plate e Bulk reagent to dispense preferably not clear e Optional Sartorius WZA225 CW balance or similar with at least a 0 001mg readability Setup a dispense program in the Micro10x to dispense 100ul into the 96 well plate Make sure to prime the system properly so the unit is dispensing the bulk reagent without any air bubbles Dispense into the entire plate once the unit is finished dispensing you will inspect the plate The inspect the plate you will raise the plate to eye level and visually inspect each well with the next in row A and H of the Plate You want to make sure that the fluid makes a level horizontal line across the entire plate If the fluid levels are not even remove the manifold and clean sonicate to clean Run the test again after the manifold is re cleaned Optional Before dispensing into the validation plate you will put it on the balance and tare the weight of the empty plate Now take the plate and place it on the Micor10X nest and run the validation program Once the program is completed you will weigh the plate on the balance The Plate should weigh the following luL of water Img A 100ul well 96 well plate 9600mg 2 tolerance 100ul Plate sh
11. nto all microplates The Micro10x provides a combination of fast filling speeds high accuracy and extreme flexibility not found in other benchtop reagent dispensers The Microl0x accurately dispenses volumes down to luL per channel and provides features like tip touch to ensure a clean full dispense of even high surface tension liquids Its positive displacement pump head is adjustable in 10nL increments and never requires calibration The Microl0x can be loaded and operated manually but is also fully compatible with robot loading systems such as Hudson s PlateCrane or track based microplate delivery systems such as Hudson s LabLinx for unparalleled throughput and ease of setup The Micro10x brings these advantages to lab applications e Conserves bench space with its small 7 x10 footprint e Graphical Image Row Selection to control the dispening pattern e Deep well filling with controlled rise to avoid bubbles or foaming e 96 384 1536 filling std and deep well e Tip Touch to control droplets e Autoclavable fluid path assures sterility Warning To prevent fire do not use Micro10x to dispense any type of flammable liquid Section 1 Setup 1 1 Tubing The MC10X is shipped with two tube assemblies Figure 1 1 1 The tube assembly with the two Flangeless fittings should be installed between the right port on the pump head and the MC10X manifold To create a proper seal tighten the fittings into the
12. ould weigh 9800mg 9400mg 28 Section 5 Hardware Specifications Section 5 1 General NOTE Specifications are subject to change without notice Micro10X Plate Format Housing Material Plate Nest Material Number of Axes Section 5 2 Mechanical Description X axis X axis Travel Distance X axis Stepper Motor Y axis Y axis Travel Distance Y axis Stepper Motor Z axis Z axis Travel Distance Z axis Stepper Motor Pump Module Material Pump head Housing Material Pump Material 96 384 or 1536 well plates in any shallow Medium or Deep well formatted SBS plate Painted steel covering anodized cast aluminum housing Peek Three X Y and Z axes Integrated stepper motor stepper controller with encoder This 1s a belt driven axis with pulley 2 hard stop to hard stop Integrated 2048 encoder steps per revolution stepper motor 0 013mm resolution approximately L second per revolution Stepper motor linear actuators with 250 count encoder Actuator has integrated 1 2 pitch Leadscrew Controlled by separate stepper controller with encoder option 5 GT hard stop to hard stop 1000 encoder steps per revolution linear actuator 0 026mm resolution approximately L second for entire travel distance Stepper motor linear actuator Actuator has integrated 9 74mm pitch Leadscrew Controlled by separate stepper controller 3 32 hard stop to hard stop 200 steps per revolution lin
13. pump head and manifold until they stop finger tight Once the fitting 1s finger tight turn the fitting an additional 1 8 turn to ensure a proper seal Figure 1 1 2 Install the longer tubing assembly in the same manner with the single Flangeless fitting between the left port on the pump head and your reagent reservoir Figure 1 1 3 Tubing Pump to Reservoir Tubing Pump to Manifold Figure 1 1 1 Figure 1 1 2 ef D 2 Left pump port La ES Figure 1 1 3 The priming trough uses a short silicon drain tube and waste basin The silicon tube can be replaced with a longer one 1f needed Standard silicon tubing will work as a replacement Figure 1 1 4 Priming Trough Figure 1 1 4 1 2 Plate Nest Install the plate nest onto the nest holder The legs of the plate nest should fit snugly into the mounting position The plate nest can be positioned in either the portrait or landscape orientation In Figure 1 2 1 you will see the plate nest in the landscape orientation You will need the 8 channel manifold to be installed 1f using the MCIOX in the portrait orientation Figure 1 2 1 1 3 Manifold Install the manifold with the inlet port closest to the pump head Use the provided thumbscrews to secure the manifold onto the mounting flange Figure 1 3 6 Figure 1 3 6 1 4 External Cables The Micro10X Comes with a few cables that need to be installed into the rear of the unit before the unit can be turn
14. ume that will be primed through the system Enter a value between 120 and 9999uL Speed This adjusts the percentage of the pumps maximum speed More viscous liquids will require a slower pump speed to avoid cavitation Do not exceed more than 30 speed on any value over 1000ul Tip Touch Activate or deactivate tip touching after each dispense Tip Touch Adjust where in the Priming trough you would like to touch the tips after a prime This is helpful when running viscous liquids 2 5 Empty Dispenser This feature reverses the pump removing most of the liquid from the manifold and tubing and returns it to the source container Figure 2 5 1 13 Figure 2 5 1 2 6 Auto Prime Priming can be programmed to occur at a user specified interval This allows the MCI10X to dispense a certain amount of fluid into the priming trough to prevent evaporation during every specified time interval Setting the time to O turns off this feature Fig 2 6 1 14 AutoPrime Time O sec volume 1000 uL EE SO Figure 2 6 1 Time This is the interval in seconds a time of O deactivates this feature Volume This is the total volume that will be moved through the manifold Enter a value between 120 and 9999uL Speed This adjusts the percentage of the pumps maximum speed More viscous liquids will require a slower pump speed to avoid cavitation Do not exceed more than 30 speed on any value over 1000ul 2
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