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2. lt gt Hed 95 000 40 logenics com www customb CLEANING amp MAINTENANCE Cleaning Exterior To clean the exterior use a mild non abrasive household type cleaner mixed with warm water Apply by using a damp cloth that you soaked in the cleaning solution and wrung out Do not expose LCD Display to liquids as this may cause damage Sanitizing and Decontamination of Interior When sanitizing the interior a mixture of 3096 chlorine bleach with 7096 water solution is considered the best method for decontamination However any household detergent or mild soap solution is suitable and can be used This includes bleach detergents and mild soaps Other cleaners and disinfectants that can be safely used include hydrogen peroxide and denatured alcohol NOTE DO NOT USE ANY PETROLEUM BASED CLEANING SOLUTIONS To perform this sanitizing procedure cover all inner surfaces with the solution let stand for 30 minutes and remove Spraying the solution into the inner vessel is preferred although agitation of the solution inside the inner will suffice Rinse the decontaminated surfaces with clean water and make sure all cleaner residues have been removed Allow the tank to dry completely before putting back into service If you have any questions concerning decontamination process please contact Custom BioGenic Systems Technical Support 1 800 523 0072 System Check 1 Testall alarm functions
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4. modicum Fill Valve V To Bypass Valve Back of Side Panel gt 3952157 2 4 6 8 10 12 14 16 1 Sequential Fill Output Black 11 Lid Temp A 0 5V Gray 2 Sequential Fill Output White 12 Lid Temp A 0 5V White 3 Sequential Fill Input Red 13 Lid Temp B 0 5V Brown 4 Sequential Fill Inut White 14 Lid Temp B 0 5V Pink 5 Lid Temp A 4 20 mA Green 15 LN2 Level 0 5V Orange 6 Lid Temp A 4 20 mA White 16 Lid Temp B 0 5V Purple 7 Lid Temp B 4 20 mA Blue 17 Thermal Printer Port Ground Green 8 Lid Temp B 4 20 mA White 18 Thermal Printer Port RX Black 9 LN2 Level 4 20 mA Yellow 19 Thermal Printer Port CTS Brown 10 LN2 Level 4 20 mA White 20 Thermal Printer Port TX Red www custombiogenics com 38 LO93 HOO9S K quuessy 1u04J 8 109J LZ957 K quuassy Z 9 1093 0 95 7 K quuessy yams Avy S pIOJTd109g emen 9529 J9 041u0 0006 t SEU TOUT 022 6 LO9I PLOG eee K quuessy 950 c 1003 1095 7777777 7777 000 1 19quunN Hed uondu saqg qI LOEZ C E3 lt gt 39 iogenics com www customb LC00 LOOM JaPUd 5 5 2 1 vC 0903 10957 F
5. rature Recorder Tempe 2301ISOCLN2 TM Rev D O 2001 2006 Custom BioGenic Systems All designs and materials contained herein are protected under Federal copyright law Unauthorized distribution or use will be subject to prosecution to the fullest extent of the law
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7. FAIL or SKIP is selected on display 841A This menu is for testing the lid temperature Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41 This menu is for testing the lid temperature Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 418 2 This menu displays current vent temperature Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41C This menu tests printer Controller advances to next test when PASS FAIL or SKIP is selected gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button if Press this button if Press this button to activate the Bypass test passes to test fails to advance skip test advance Valve for as long as advance to display to display 41F and to display 41F the button is held 41 and next test next test and next test below below below 41F Press this button to Press this button if Press this button if Press this button to activate Fill Valve test passes to test fails to advance skip test advance for as long as the advance to display to display 416 and to display 416 button is held
8. M q uone iunululo JONUOWOAID S8r SY 101 pesf LO S8r SY S19 O1JUOD 7 JONUOWOAID S8p SY JO P SN NI 68 58 55 woisuedxe 19449433 94 340d 3 3 3NVd 3415 43 TIOULNOD LOEC 531435 tN lt gt EBS www custombiogenics com V SERIES CAROUSEL MANUAL FILL INSTRUCTIONS The unique design of the new Isothermal Storage Unit is such that it is no longer necessary to fill the storage chamber with liquid nitrogen Instead the inner walls of the storage vessel are filled with liquid nitrogen and the sample storage chamber holds no liquid at all Because of this unique design the manual filling of this equipment is very different from storage units of the past Use the following instructions to manually fill the Isothermal Carousel Storage unit in the event of a power outage or if the auto fill is not working properly For the correct manual filling of the Isothermal unit perform the following steps 1 Turn off the liquid nitrogen supply valve at the supply tank 2 Disconnect the supply line from the Auto fill port on the back of the unit AUTO FILL CONNECTION 3 Remove the cap from the manual fill port also on the back of the unit MANUAL FILL CONNECTION 4 Place the cap on the auto fill port where the supply line was connected and tighten down 5 Connect the liquid nitrogen supply line to t
9. operation under most circumstances THE TEXT TO THE RIGHT OF THE DISPLAY EXPLAINS THE SPECIFICS OF THE DISPLAY MESSAGE AND THIS NUMBER TO THE LEFT OF EACH WINDOW IS A GENERAL IDENTIFICATION NUMBER USED TO ORGANIZE THE DISPLAYS FOR THIS MANUAL THIS NUMBER WILL NOT Press this button to Press this button to begi fill advance to display 99 CONTROLERORTHEACTUAL andpm WHY IT IS OCCURRING DISPLAY WINDOWS see page 13 THE TEXT BELOW THE BUTTONS EXPLAIN THE FUNCTION OF EACH OF THE BUTTONS ALSO TELLS WHICH DISPLAY THE BUTTON WILL ADVANCE TO AND THE PAGE NUMBER WHERE IT CAN BE FOUND IN THE INSTRUCTION MANUAL Standard Operating Mode Display Windows This is the standard display 1 which will appear during normal operation under most circumstances NOTE Use Up Down arrows to toggle display between Temp A and Temp B p D Cf JC JC OC This display will appear during normal operation when either a manual or automatic fill is occurring www custombiogenics com 14 lt gt es LCD DISPLAY FUNCTIONS Standard Operating Mode Display Windows This display will appear during normal operation when the lid is opened Press this button to Press this button begin a manual fill stop filling This display will appear when an alarm condition is occurring The specific nature of the alarm will be shown in li
10. 4106 and next test next test and next test below below below 4106 Press this button if Press this button if Press this button to test passes to test fails to advance skip test advance advance to display to display 41 and to display 41H 41H and next test next test and next test below below below 41H Press this button to Press this button to print test results and return to display 6 MAIN MENU 3 see page 16 return to display 6 MAIN MENU 3 see page 16 www custombiogenics com 22 This display will appear when PASS FAIL or SKIP is selected on display 410 This menu tests the fill valve Controller advances to next test when PASS or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41E This menu tests the fill valve Controller advances to next test when PASS or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41F This menu tests the lid switch With the lid closed the display should read LID IS NOW CLOSED with the lid open it should read LID IS NOW OPEN Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when the final test button is selected on display 416 and when all tests have been completed or skipped Use this menu to print test results if desired
11. Disconnected sensor tube see 7 on page 7 EH settings are enable on a non EH unit High pressure supply Blown fuse No power at wall outlet AC power cord not plugged in ON OFF switch in OFF position Obstructed pressure port Loose sensor hose Unit out of calibration Remote alarm wires connected to the wrong terminals 33 SOLUTIONS Shut off manual valve on liquid supply wait for the liquid to settle and do one of the following Carefully bail liquid out of vessel using approved cryogenic container and proper personal safety equipment or Wait for liquid to evaporate from vessel on it s own Shut off manual valve on liquid supply wait for the liquid to settle and do one of the following Carefully bail liquid out of vessel using approved cryogenic container and proper personal safety equipment or Wait for liquid to evaporate from vessel on it s own Replace with two amp fuse Use live power source Plug the AC power cord into a wall socket Move power key switch into the ON position Clear blockage from pressure port in bottom of vessel Check sensor hose connection Calibrate unit using the calibration guide on page 29 30 Reconnect wiring using the guide on page 12 Remote Alarm Wiring gt E lt gt i ay Y 590 Punim 9JIM
12. 9000 10037 TT 6 JEW 10957 TT 55 ETT T 49104340 L093 Zv9g7 77777 f qUuessy snd dWY Z GEOO 10037 pH urens 1093 9799 1 quuessy 9126 peauued 7 lt 9 e2u329 3 LOEZ 1093 0 95777 77777 qe pue J0129UUO S98t SH 1093 6599 96 pue 10129UUO 39449433 Er i T9 TUI Hed uondu seg OSN _ e e gt 8 L 9 S lt gt gt OO NN 36 logenics com www customb T1 Med pued LOEZ 2301 Controller Wiring Diagram Part I D Top View Under Clam Shell ya FER C poe SU Back of Side Panel 1 Relay Com Wire Red 7 Remote Alarm COM Wire White 2 Relay Com Wire Black 8 Remote Alarm NO Wire Blue 3 USB Cable Black 9 Remote Alarm NC Wire Red 4 RS 485 Output Cable Gray 10 24V DC Input From Key Switch Black 5 RS 485 Input Cable Gray 11 From Fuse To Key Switch Black 6 Ethernet Cable Blue 12 From 24V DC Board Connection to Fuse Black www custombiogenics com 37 C_B _ 2301 Controller Wiring Diagram Part I D Top View Under Clam Shell pum ju 4 To Manifold Tank b S
13. check to see that the valve is plugged in and or that there is power to the outlet which the valve is plugged into If power is present at the valve but the vessel is still not filling with liquid nitrogen the valve may be defective An automatic fill will begin and when the liquid level rises above the LOW level setpoint and the pressure port the alarm will subside Check pressure hose at all connection points a white clamp should be in place at each end of the hose inside the controller and at the hose connection on the back of the controller If hose appears loose disconnect it remove an inch off the end by cutting it with scissors and reconnect it If the vessel is holding liquid it may take some time to read the corrected level gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS SOURCE text is flashing and audible alarm is present ALARM SOURCE ALARM This indicates a weak or empty liquid supply Liquid level is above the LOW level set point but isn t rising fast enough to satisfy the system TEMP A text is flashing and audible alarm is present ALARM TEMP A NOTE Text may display Temp B OPEN FILL text is flashing and audible alarm is present ALARM OPEN FILL www custombiogenics com CAUSES Supply tank is empty Supply tank is shut off Supply tank pressure is too low or to high 3 or more storage units are filling at one ti
14. is done by adjusting the level set points at lease one inch below the actual liquid level If necessary refer to page 18 display 15 and 16 Example The liquid level is 6 inches Adjust the low set point to 2 and the high set point to 4 In 3 to 5 minutes a high alarm should occur This will be indicated by the HIGH ALARM text flashing and the audible alarm sounding The remote alarm output will activate also 2 Return set points to the desired settings Unit is now ready for normal operation If the alarm functions aren t occurring as described above repeat the verification procedure If one or both of the alarms are still not functioning properly refer to the troubleshooting guide on page 36 for further instruction Temperature Display Verification 1 Open Carousel storage unit Access Panel and locate temperature sensing probes A and B Access Panel 2 Remove probe from protective sleeve 3 Submerge the sensor probe into liquid nitrogen The digital temperature display should read 195 to 196 degrees celsius 4 Remove probe from liquid nitrogen and place tip of probe in an ice bath mixture of ice and water and wait 30 seconds While the probe is submerged the digital display should read 1 to 1 degrees celsius 5 Return probe into its protective sleeve at the desired depth 6 Close the Carousel storage unit Access Panel and resume normal operation If the temperature display doesn t oper
15. operation the sequence of events is as follows When the liquid level in the vessel falls below the low level set point a signal is sent to the controller At this time the FILL solenoid valve is activated allowing the flow of liquid into the vessel The Bypass Function will activate the VENT solenoid first to release gas or vapor from the transfer lines The venting will continue until the line temperature reaches approximately 150 to 160 C This can be adjusted consult the factory for more information When the line reaches this temperature the vessel will begin to When the liquid level reaches the high level set point a signal is sent to the controller which deactivates the solenoid valve and stops the flow of liquid into the vessel As long as the unit is powered up this fill sequence repeats as necessary maintaining the desired liquid level Check unit daily to ensure proper operation and safety of the stored samples For the V Series Carousel units it is essential to lift the lid each day and check for vapor and signs of proper freezing ae www custombiogenics com 5 C E L5 FRONI PANEL CONTROLS www CustomBioGenics com 1 Key Switch Power Control Main power control for the unit 2 LCD Display Window Text interface for the storage unit electronic settings See page 13 for complete listing of all display menus and functions 3 First Menu Select Button This is used to select various contro
16. the supply tank to the storage freezer but do not open the manual supply valve until instructed to do so Connect Remote Alarm or Auto Dialer if desired Plug unit into an appropriate power source Turn the power key switch to the ON position and the program lock to program Adjust the Liquid Level Set points See display 4 on page 16 for instructions press LIQ D level Adjust the High Temperature Alarm Set point after filling is complete See display 5 on page 16 for instructions press TEMP 7 Open the manual valve on the supply tank Check for leaks at the connection points then proceed to normal operation OPERATION The V Series Carousel Storage Units are designed to maintain liquid nitrogen levels within specified parameters inside the walls of a cryogenic storage vessel This is achieved by measuring gravitational weight of the liquid nitrogen with a pressure sensing unit in the controller The controller activates the FILL solenoid valve when the liquid level goes below the LOW level set point and deactivates the solenoid valve stopping the fill when the level reaches the HIGH set point The Bypass Function has an additional solenoid valve to vent vapor from the transfer lines before the FILL solenoid is activated In the description below the part of the sequence which is unique to the Bypass Function is in brackets All other information refers to units with or without the Bypass Function During regular
17. to display 5 return to display 5 MAIN MENU 2 MAIN MENU 2 see page 16 see page 16 www custombiogenics com 19 This display will appear when F 15 selected on display 19 Use this menu to select a temperature for the Temp A Alarm to be activated This display will appear when F 15 selected on display 19 Use this menu to select a temperature for the Temp B Alarm to be activated This This display will appear will appear when REPORT is selected on display 5 Use this menu to select the time interval between temperature reports NOTE A value of means temp report printing is disabled For use with thermal printer only This O This display will appear when will appear when ENTER is selected on display 22 Use this menu to select number of days between temperature reports NOTE A value of means temp report printing is disabled For use with thermal printer only gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows This display will appear when BYPASS is selected on display 5 This menu is a prompt to enable or disable the Bypass Option Press this button to Press this button to change current setting maintain current setting and return to display and return to display 4 MAIN MENU 4 MAIN MENU see page 16 see page 16 This display will appear when 40 VALIDATION is
18. 2 amp max Fuse VAC 2 amp Buss fuse 220 volts slow blow T2A 250V automotive glass tube type fuse Specification V3000 AB C 5000 5000 LN2 Capacity Static Holding Time Days ERE IR External Dimensions i O Width szemr 09m T 80203 ea 7307 585 3827 Usable Interior Height 27 686 35 889 Usable Interior Diameter 29037 29073 Weight Empty lbs ks 900072 10 Weight Full bs kg 82772208 Maximum Vial Capacity 2m 18000 22500 Maximum Blood Bag Capacity 85222 1462 Operating levels for LN Extra High Series 0 to 28 inches higher fill heights may be used call CBS for more info V Series Models 23 inches www custombiogenics com 12 ee SS LCD DISPLAY FUNCTIONS This section shows each display window and gives important information necessary for understanding and operating the LCD text functions of the 2301 Controller To start the index below shows where in this section to find each and every function of the controller This will enable the user to correctly program all functions of the storage unit to meet their individual needs The overall operation consists of two basic modes the Standard Operating Mode and the Program Mode The unit is in the Standard Operating Mode when the Program Lock key switch on the front pane
19. AB V5000 AB C V5000EH AB C are intended for use in the electromagnetic environment specified below The customer and user of these products should assure that they are used in such an environment Emissions Test Compliance Electromagnetic environment guidance The Cryo freezer uses RF energy only for its internal function RF Emissions CISPR 11 Group 1 Therefore its RF emissions are very low and are not likely to cause any interference in nearby electronic equipment RF Emissions CISPR 11 The Cryo freezer 15 suitable for use in all establishments other Harmonic emissions IEC Class than domestic and may be used in domestic establishments and those 61000 3 2 directly connected to the public low voltage power supply network that supplies buildings used for domestic purposes provided the following warning is heeded Warning This equipment system is intended for use by healthcare Complies professionals only This equipment system may cause radio interference or may disrupt the operation of nearby equipment It may be necessary to take mitigation measures such as re orienting or relocating the Cryo freezer or shielding the location Voltage fluctuations flicker emissions www custombiogenics com 42 Ce Ss 5 www custombiogenics com 43 Ce Ss rs ms R Freezing syste ntro oxes Freezer Bo i Transfer Line solenoid V larms erature A s vel amp Tem e Contro Liquid pie g Tempera tors
20. CUSTOM BIOGENIC SYSTEMS ISOTHERMAL CAROUSEL FREEZER USER MANUAL Custom www custombiogenics com BioGenic 74100 Van Dyke Bruce Twp MI 48065 USA GB Systems 500 523 0072 Tel 1 586 331 2600 Fax 1 586 331 2588 Leading the World with Innovative Cryopreservation Technology Solutions European Authorized Representative EMERGO GROUP Molenstraat 15 2513 BH The Hague The Netherlands Custom Biogenic Systems liquid nitrogen freezers equipped with 2301 controllers are CE marked to the Medical Device Directive The Medical Device Directive is a European Union directive regulating medical device construction and operation C 0086 Custom BioGenic Systems id 74100 Van Dyke Road Bruce Township MI 48065 European Authorized Representative EMERGO GROUP Molenstraat 15 2513 BH The Hague The Netherlands Custom Biogenic Systems maintains ISO 13485 2003 Quality Management System This manual covers the use of Custom Biogenic Systems Liquid Nitrogen Freezers equipped with 2301 controllers This equipment is intended for use by trained personnel only READ THE MANUAL BEFORE USING THIS EQUIPMENT Custom Biogenic Systems Liquid Nitrogen Freezers covered in this manual are non hazardous open mouth vacuum insulated dewars specifically designed for use with liquid nitrogen They are no subject to any pres sure vessel codes as they are open to atmospheric pressure They are shipped empty without liquid nitro
21. ERFLOW will appear on the display and power to all solenoid valves will be disabled www custombiogenics com 11 REMOTE ALARM WIRING Locate the Remote Alarm Connector on the controller 10 on page 7 amp 8 This is where the remote alarm or auto dialer will plug into the control unit All Global Alarm connections are dry contact relays with a max output 250 VAC 5A The Remote Alarm Connector is a Dry Contact This Diagram shows connections for wiring arrangement Common Normally Closed Normally Open First wires from the remote alarm or auto dialer must be connected to the plug end which plugs into the Remote Alarm Connector Use the diagram to connect wires from the remote unit Alarm connector jack on unit bdck panel Push into only goes one way p note Alarm connector to push inside pin after wiring When unit goes into an alarm condition or loses power the contacts will switch their Open or Closed state to be the opposite of when there was not an alarm condition thereby sending a signal to the remote alarm or auto dialer Wiring it to common and normally open will trigger the remote contact if a power failure occurs STORAGE UNIT PARAMETERS Cooling medium Liquid nitrogen only supplied by a pressurized cryogenic transport vessel Operating Pressure 22 p s i Power Supply Voltage 120 Volts AC 60 Hz standard 220 Volts AC 60 50 Hz optional Amperage
22. OUS MODE Connect all 2301 controllers to your LN2 supply source Select Simultaneous mode All freezers will begin to fill at the same time until all linked controllers have reached their High Level Set Point simultaneously Custom BioGenic Systems recommends selecting Sequential This mode allows freezers to fill one at a time with a primed and cold supply system making it easier to maintain proper filling pressure and reducing LN2 transfer loss This is more efficient than a keep cold system For best results with large pipeline LN2 supply systems adjustment to the source alarm timer may be required Please contact Custom BioGenic Systems with any questions regarding the function and setup of the SEQ OFAF feature In the event of an alarm status that stops the freezer from reaching the High Level Set Point the 2301 controller will not trigger the next freezer in line to fill However the auto fill function always takes priority over the SEQ OFAF settings SEQUENTIAL FILL SIMULTANEOUS FILL TANK1 2 TANK3 4 TANK1 2 TANK3 4 fra on 2 moe oa a LL LL LL www custombiogenics com CEB S 10 HL LL HL LL HL LL FEATURE DETAILS Fill Timer See page 16 screen 6 Enables setting the timer to fill the freezer at 24 48 or 72 hour intervals at the time selected Note that the Auto Fill setting will al
23. advance to display advance to display to display 41B 418 and nexttest 418 and next test and next test see page 21 see page 21 see page 21 www custombiogenics com 20 CBS LCD DISPLAY FUNCTIONS Program Mode Display Windows u Em Press this button if Press this button if Press this button to test passes to test fails to advance skip test advance advance to display to display 41B 2 to display 41B 2 41B 2 and next test next test and next test below below below Press this button if Press this button if Press this button to test passes to test fails to advance test advance advance to display to display 41C and to display 41C 41C and next test next test and next test below below below hm Press this button if Press this button if Press this button to test passes to test fails to advance skip test advance advance to display to display 410 and to display 410 410 and next test next test and next test below below below BEEN Press REN button to send TESTING PRINTER to the Press button if Press amu buttonif Press button to test passes to test fails to advance skip test advance advance to display to display 41 and to display 41E printer 41E and next test next test and next test see page 22 see page 22 see page 22 www custombiogenics com 21 This display will appear when PASS
24. ate as described there may be a problem with the probe or the probe connections See the Troubleshooting Guide on page 36 for further information if a problem is detected www custombiogenics com ea SS 2301 TEMPERATURE CALIBRATION TEMP PROBE A Satisfy alarm conditions so that the controller is in its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point 2 Place Temp A thermocouple probe in Ice Bath and adjust trimmer P8 until display reads 0 deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up 3 Place Temp A thermocouple probe in Liquid Nitrogen and adjust trimmer P9 until display reads 196 deg C Clockwise adjusts temp display down Counter clockwise adjusts temp display up 4 Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree TEMP PROBE B 1 Satisfy alarm conditions so that the controller is in its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point 2 Place Temp B thermocouple probe in an Ice Bath and adjust trimmer P10 until display reads O deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up 3 Place Temp B thermocouple probe in Liquid Nitrogen and adjust tr
25. ay number 13 page 18 Liquid Level Settings Display number 15 page 18 Main Menu 2 Display number 5 page 16 Provides access to Temp Settings Display number 19 page 18 Printer Functions Display number 22 page 19 Bypass Settings Display number 24 page 19 Main Menu 3 Display number 6 page 16 Provides access to Control Validation Display number 40 page 20 Fill Timer Settings Display number 7A page 17 Software Revision level Display number 42 page 23 Main Menu 4 Display number 7 Page 16 Provides access to One Fill All Fill Display 44 page 24 Log Display 45 on Page 23 Return to Main Menu Display 4 on page 16 www custombiogenics com 13 CBS LCD DISPLAY FUNCTIONS Using The Diagrams to Understand the Display Functions WARNING NEVER USE AN OBJECT OTHER THAN YOUR FINGER TO PRESS THESE BUTTONS DOING SO WILL RESULT IN PERMANENT DAMAGE TO THE CONTROLLER OVERLAY Below is a sample display window as they re shown in the following section Use the diagram to become familiar with the way this section is organized and how the information is presented in reference to each display window and it s functions NOTE Tolerance on Liquid Level Display is 1 2 54cm and The Tolerance on the Temperature display is 1 SAMPLE DISPLAY WINDOW X s ARE USED IN PLACE OF NUMBERS IN THE DISPLAY WINDOW SINCE THE ACTUAL NUMBERS WILL VARY This is the standard display which will appear during normal
26. basic explanation of the mechanics of the system Also be sure to read this manual completely before attempting to use this equipment If you are still not sure how to use this equipment properly contact a competent authority rather than risking your safety or the safety of the stored samples Patented Liquid Nitrogen Jacket there is no liquid nitrogen in the storage space When the unit is placed into service check daily to ensure proper operation and safety of the stored samples For the V Series carousel units it is essential to lift the lid each day and check for vapor and signs of proper freezing www custombiogenics com 4 C E3 lt amp INITIAL SET UP This equipment is designed to operate in the following environmental conditions Temperature 5 C 41 F to 40 C 104 F Humidity 80 at lt 31 C decreasing linearly to 50 at 40 C Altitude lt 2 000 meters 6 650 feet As a first step in the set up procedure be sure to read this manual completely to become more familiar with this equipment Next remove all packing materials and wipe unit with a clean cloth inside and out to remove any debris left from shipping Once the unit is wiped down complete the following Steps Note The Temp A Temp B alarm is set at 30 C from the factory Thus it will not alarm during the first fill and must be set by the user once the freezer cools 150 C 1 Connect the LN stainless steel flexible hose from
27. d Best Practices in the industry for safe sample storage Secondary or backup alarm It is strongly recommended to have at a minimum an Independent Temperature Alarm for each LN2 freezer Remote alarm connection It is strongly recommended to have at a minimum a Remote Auto Dialer connected to your Delta Room Facility Monitoring Station or Remote Auto Dialer for each LN2 freezer Split Samples Separate samples in to two different freezers Keep a daily log Track temperature levels daily Record fill intervals and amount of LN2 filled into vessel manual or pour fill freezer or dewar This information should be documented daily and reviewed monthly to foresee and prevent future problems such as temperature fluctuations and varying liquid levels Prepare a contact list Have at least 3 people on your contact list with Home Cell and Pager Number Review the list regularly for accuracy and changes Contingency plan What to do if no one returns a call from your call list What to do if we run out of LN2 and it is a weekend or holiday What to do if you have a High Temp Alarm What to do if you have a Low Level Alarm What to do if you have a High Level Alarm What to do if you lose power What to do if you get a Source Alarm www custombiogenics com C E L5 QUESTIONS and ANSWERS Keep your most important samples safe You can plan for the future you can t change the past Back up or secondary Temp Alarm Q Why do I need a Sec
28. d LN2 in a jacketed space between the vacuum insulated space and storage chamber it cannot be calibrated the same as S Series storage units Customers have multiple options to choose from in how they want to go about calibrating their Isothermal storage unit For all options below please call for pricing and further details N I S T traceable options Purchase Calibration Equipment Rent Calibration Equipment Have a qualified technician sent to your facility to perform the calibration Return the controller to Custom Biogenic Systems to be calibrated gt t www custombiogenics com 28 CB Ss 29 TROUBLESHOOTING GUIDE ALARMS CONDITIONS HIGH ALARM text is flashing and audible alarm is present ALARM HIGH ALARM Liquid level in the vessel has risen above the HIGH level set point LOW ALARM text is flashing and audible alarm is present ALARM LOW ALARM Liquid level in the vessel has fallen below the LOW level set point www custombiogenics com CAUSES Check to see if unit is filling If unit is filling If unit is not filling Supply tank is empty Supply tank is shut off Supply tank pressure is too low 3 or more storage units are filling at one time from a single supply line 29 SOLUTIONS Push the STOP button if fill continues the solenoid valve may be frozen open push the FILL and STOP buttons 15 to 20 times to un stick the valve If the fill cont
29. for proper operation 2 Checkany connected Remote Alarms or Automatic Dialing Systems to ensure proper operation See page 26 for detailed instructions on how to manually cause a HIGH ALARM or LOW ALARM to test the remote alarm contacts 3 Checkfor leaks at all connection points of the liquid nitrogen lines 4 sure that all electrical wires are free of damage and plugs are firmly in place The solenoid valves may be opened and cleaned if they are not working smoothly If this is done use the diagram below to re assemble the valves properly NOTE If the valve is found to be defective the entire valve must be replaced individual parts are not available 1 Valve Body 2 Plunger 3 Flange 4 Valve Coil Locknut 5 Coil 6 Spec Plate 7 Valve Cap www custom biogenics com 41 CBS Guidance and manufacturer s declaration electromagnetic immunity The CBS family of large LN2 freezers including the V1500 AB 51500 V3000 AB V3000EH AB V3000 AB C V3000EH AB C 53000 V5000 AB V5000 AB 5000 55000 SSOOOEH AB V5000 AB C 5000 are intended for use in the electromagnetic environment specified below The customer and user of these products should assure that they are used in such an environment Immunity test Hat rcc Electromagnetic environment guidance Floors should be wood concrete or ceramic tile If Complies floors are covered with synthetic material the re
30. gen or any hazardous material from out factory in Michigan Custom Biogenic Systems liquid nitrogen freezers cryogenic eugipmetn and accessories are designed and intended for use with various applications where extremely low temperature storage is desired such as vet erinarian horticultural pharmaceutical industrial scientific and research laboratories They are not inteded fo ruse in the diagnosis of disease or other conditions or in the cure mitigation treatment or prevention of disease and as such have no medical purpose They are designed for but not limited to use in the laboratory environment Thank you for choosing Custom BioGenic Systems Custom BioGenic Systems can supply you with everything you need to effectively and safely store your samples Our 2101 controlled rate freezer will freeze your samples down at a controlled rate prior to long term liquid nitrogen storage We have an extensive selection of freezer racks bag storage solutions boxes and dividers for liquid nitrogen freezers mechanical freezers as well as custom configurations For long term storage we offer our patented Isothermal dry storage liquid nitrogen freezers carousel freezers standard liquid nitrogen freezers cryosystems and dewars in many popular sizes VersAlert will monitor your temperature critical products and send alerts via text message or email if specified parameters are exceeded For more information please contact Custom BioGenic Sys
31. gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to maintain current setting and return to display 4 MAIN MENU see page 16 These arrows 7 jJ Press this button to Press this button to save the setting and discard changes and go to display 13C return to display OUTPUT SIGNALTYPE 13B Output Signal see below Type see below These arrows adjust settings 7 Press button to save changes and return to display 4 MAIN MENU see page 16 Press this CJ to discard changes and return to display 4 MAIN MENU see page 16 WU Press this button to Press this button to save the setting and discard changes and Return to display 5 return to display 5 Main Menu 2 MAIN MENU 2 see page 16 see page 16 These arrows 7 www custombiogenics com 23 adjust settings adjust settings This display will appear when you hit the REV button on display 6 on PAGE 16 Use this menu to view your revision number and date This display will appear after a selection is made on screen 13 see page 18 NOTE If this controller is connected to the PC it is to be set to master if it is in the chain leading to the master it is to be set to slave This display will appear after a selection is made on screen 13A see above Use this menu to activate either the 4 20mA outputs or
32. hat the automatic fill will occur These arrows adj cs value This display will appear when DATE TIME is selected on display 4 Use this menu to change the date and time settings NOTE DAY MONTH YEAR gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows These arrows adjust value 13 This display will appear when TANK ID is selected 7 on display 4 The Tank ID is necessary when using COV the CryoMonitor computer Press this buttonto Press this button to interface software save the setting and discard changes and return to display 13A return to display MASTER SLAVE 113A Master Slave see page 23 see page 23 15 This display will appear when LIQ D LEVEL is selected on display 4 Use this menu to select measurement C C preferences for the liquid level settings i e inches or cm Press this button to Press this button to Press this button to select INCHES and select CENTIMETERS discard changes and advance to display 16 and advance to return to display 4 below display 16 MAIN MENU below see page 16 These arrows i TE display 16 INCHES or CENTIMETERS is selected on display 15 Use this menu to select and LOW liquid level settings Press this button to Press this button to Press this button to Press this button to save settings and move cursor to movecursorto discard cha
33. he manual fill port and tighten down www custombiogenics com 9 C E L5 V SERIES CAROUSEL MANUAL FILL INSTRUCTIONS Without power there will be no digital display to give the liquid level and the liquid cannot be seen visually since it is contained inside the walls of the Isothermal Unit Thus the lid must remain open during the manual fill process With this in mind the best way to gauge the fill level is when the tank is nearing maximum fill liquid nitrogen will begin to spray from the vents located near the top edge of the inner walls of the vessel When this is seen the supply valve should be shut off immediately before excessive amounts of liquid nitrogen begin to spill into the storage space VENTS Check unit daily to ensure proper operation and safety of the stored samples For the V Series units it is essential to lift the lid each day and check for vapor and signs of proper freezing www custombiogenics com 9a C E3 lt gt es FEATURE DETAILS SEQ OFAF system See page 16 screen 7 SEQUENTIAL MODE recommended Connect all 2301 controllers to your LN2 supply source Select Sequential mode to maintain optimum fill pressure and significantly reduce LN2 transfer loss Once the first controller reaches its High Level Set Point it will activate the next controller This process will continue until all linked controllers have reached their High Level Set Point sequentially SIMULTANE
34. ill appear when OFAF function is set to SIMULTANEOUS or SEQUENTIAL see page 24 display 44 and the ENTER button was pressed Note Factory settings should be kept any questions please contact Custom BioGenic Systems This display will appear after screen 46 see above Note Factory settings should be kept any questions please contact Custom BioGenic Systems COC BS HIGH amp LOW LEVEL ALARM AND TEMPERATURE DISPLAY VERIFICATION The following section includes verification procedures for the High and Low Level Alarms and the Temperature Display on the Isothermal Carousel Liquid Nitrogen Storage Units Low Level Alarm Verification 1 Using the manual valve on the liquid nitrogen supply tank turn off the liquid supply 2 Create a low level alarm condition This is done by adjusting the level set points above the actual liquid level If necessary refer to page 18 display 15 and 16 Example The liquid level is 6 inches Adjust the high level set point to 10 and the low level set point to 8 This will cause the controller to go into an auto fill In 7 to 10 minutes low alarm should occur This will be indicated by the LOW text flashing and the audible alarm sounding The remote alarm output will activate also 3 Return set points to the desired settings 4 Open manual valve on supply tank to resume normal operation High Level Alarm Verification 1 Create a high level alarm condition This
35. immer P11 until display reads 196 deg C Clockwise adjusts temp display down Counter clockwise adjusts temp display up 4 Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree BYPASS TEMP 1 Satisfy alarm conditions so that the controller is in its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point 2 Place BYPASS thermocouple probe in an Ice Bath and adjust trimmer P6 until display reads 0 deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up 3 Place BYPASS thermocouple probe in Liquid Nitrogen and adjust trimmer P7 until display reads 196 deg C Clockwise adjusts temp display down Counter clockwise adjusts temp display up 4 Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree TEMP B TEMP A VENT TRIMMERS m zn d Uy n5 7 E RE Sewers es M LJ 457193 3 Lad 4 Go to custombiogenics com technicalguides html for more information www custombiogenics com 27 CB Ss V SERIES CAROUSEL LIOUID LEVEL DISPLAY VERIFICATION amp CALIBRATION Since the V Series storage units hol
36. inues turn off the manual valve on the liquid nitrogen supply tank and let the solenoid valve warm to room temperature then re open the manual valve to see if proper operation returns If not the solenoid valve may need to be disassembled for service see page 39 Cleaning and Maintenance There may be an obstruction in the solenoid valve Remove solenoid valve and disassemble for service see page 39 Cleaning and Maintenance Replace supply tank Open manual valve on the supply tank Check pressure in supply tank 25 p s i maximum 20 p s i minimum 22 p s i recommended Storage vessels will fill very slowly and alarm will correct itself gt continued on next page gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS CAUSES LOW ALARM text is flashing and audible alarm is present Solenoid valve operating erratically ALARM LOW ALARM The LN level in the vessel has fallen below the pressure sensing port LOW ALARM text is flashing audible alarm is present and level display reads 00 0 cm or 00 0 in ALARM LOW ALARM www custombiogenics com 30 SOLUTIONS Push STOP button briefly and listen for the fill solenoid valve to click This sound is the valve operating If the click is heard and the supply tank still has liquid and pressure in it there may be an obstruction in the valve or in the line If no click is heard
37. ith loose bulky dry and sterile dressings 2 Ifthe skin is blistered or there is a chance that the eyes have been affected seek medical help immediatley SPECIFIC FIRST AID PROCEDURES PROMPT MEDICAL ATTENTION IS MANDATORY IN ALL CASES OF OVEREXPOSURE TO NITROGEN RESCUE PERSONNEL SHOULD BE EQUIPPED WITH SELF CONTAINED BREATHING APPARATUS INHALATION Conscious persons should be assisted to an uncontaminated area and inhale fresh air Quick removal from the contaminated area is most important Unconscious persons should be moved to an uncontaminated area given mouth to mouth resuscitation and supplemental oxygen Further treatment should be symptomatic and supportive SKIN OR EYE CONTACT Flush affected areas with lukewarm water DO NOT USE HOT WATER A physician should see the patient promtly especially if the cryogenic burn has resulted in blistering www custom biogenics com 3 CES V SERIES CAROUSEL DESCRIPTION Throughout this manual references are made to the V Series Carousel storage unit vessel In order to give a complete understanding of the carousel model the diagram at the right is provided With the V Series Carousel model the liquid nitrogen is contained inside the vessel walls and the stored samples inside the carousel never come into contact with the liquid nitrogen except in the case of an accidental overfill To understand the way the system operates see the OPERATION section on the next page for a
38. l Page 6 number 9 is turned to Lock To enter the Program Mode the key must be inserted and the switch turned to Program In the Standard Operating Mode there are 6 different displays possible They are as follows Standard Display Display number 1 page 14 Standard Display While Unit is Filling Display number 2 page 14 Standard Display With Lid Open Display number 3 page 15 Alarm Condition Display Display number 90 page 15 Alarm Condition Display With Audible Alarm Muted Display number 91 page 15 Printing Report Display number 99 page 15 The Alarm Condition display will give the specific cause of each alarm condition that may be occurring The following is a list of the possible alarm conditions Low Alarm Open Fill High Alarm Open Bypass Source Alarm Temp A Probe Temp A High Temp B Probe Temp B High Open BP Probe Thermistor Open LN2 Overflow Optional Additional Equipment Required In the Program Mode there are various displays possible The following is a list of programming functions and the corresponding display windows For each of these programming functions there may be sub menus that follow the primary display window The sub menus are not listed here but are shown and explained following the primary windows The primary programming functions are as follows Main Menu Display number 4 page 16 Provides access to Date Time Settings Display number 8 page 17 Tank ID Settings Displ
39. l menus and to make changes in programming as shown by the text which appears above the button 4 Second Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 5 Third Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 6 Fourth Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 7 Scroll Down Button Scrolls to lower numbers when programming electronics 8 Scroll Up Button Scrolls to higher numbers when programming electronics 9 Program Lock Key Switch Switches the controller between the Standard Operat ing Mode and the Program Mode Also protects electronic programming from being changed by unauthorized personnel www custombiogenics com 6 C E L5 ON iae CLOO ETS OOF suo 01 68 C W s 59089 IW d car 214 aul Ell axA o0LtZ G 122135 la oouiw3 SS yuacog EM ER UGG Wav TV 1LOW3MH 1v8019 Ner S irs AS A 1940 4940 Y awa 8 di mn 13 11 4 dWal 8 BAT 035 035 e lt gt gt N JAHOd 58 59
40. lative humidity should be at least 30 Electrostatic discharge ESD IEC 61000 4 2 IEC 61000 4 3 2 5GHz p ansmitters as determined by an electromag sit survey should be less than 3 V m 6kV Contact 8kV Air Interference may occur in the vicinity of equipment marked with the following symbol Conducted RF 3Vrms 150kHz to IEC 61000 4 6 SOMHz Electrical fast transient 2kV power line d power quality should be that of a typical Cures differential commercial or hospital environment IEC 61000 4 5 2kV common Power frequency Power frequency magnetic fields should be at levels magnetic field characteristic of a typical location in a typical IEC 61000 4 8 commercial or hospital environment power quality be that of a typical commercial or hospital environment If the user of the Cryo Freezer requires continued operation during power mains interruptions it is recommended that the Cryo Freezer be powered from an uninterruptible power supply or battery Voltage dips short interrupts and voltage 60 dip 5 cycles variations on power supply input lines 70 dip 25 cycles IEC 61000 4 11 95 dip 5 sec Guidance and manufacturer s declaration electromagnetic emissions The CBS family of large LN2 freezers including the V1500 AB 51500 V3000 AB V3000EH AB V3000 AB C V3000EH AB C S3000 AB V5000 AB V5000 AB V5000 EH AB 55000 SSOOOEH
41. me from a single supply line Temperature has risen above set point Probe has been inadvertently moved Fill valve disconnected from power source Fill valve defective 31 SOLUTIONS Replace supply tank Open manual valve on supply tank if closed Check pressure in supply tank 25 p s i maximum 20 p s i minimum 22 p s i recommended Source alarms are not self correcting Lid has been open too long Low liquid nitrogen level Move probe to original location Connect fill valve power cord correctly using diagram on page 7 Replace fill valve gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS OPEN BYPASS text is flashing and audible alarm is present ALARM OPEN BYPASS TEMP A text is flashing and audible alarm is present ALARM TEMP A PROBE NOTE Text may display Temp B OPEN BP PROBE text is flashing and audible alarm is present ALARM OPEN BP PROBE Any ALARM text is flashing and an audible alarm is NOT present SOUND THERMISTOR text is flashing and audible alarm is present ALARM THERMISTOR OPEN www custombiogenics com CAUSES Bypass valve disconnected from power source Bypass valve defective Thermocouple probe plug is disconnected Thermocouple probe is damaged Thermocouple probe plug is disconnected Thermocouple probe is damaged There is an alarm condition of so
42. me kind and the alarm MUTE is activated Thermistor plug is disconnected Thermistor is damaged 32 SOLUTIONS Connect bypass valve power cord correctly using diagram on page 7 Replace bypass valve Connect probe plug properly using diagram on page 7 Replace damaged probe Connect probe plug properly using diagram on page 7 Replace damaged probe Correct the alarm condition and then push the SOUND switch so the audible alarm will be activated at the next alarm occurrence Connect Thermistor properly using diagram on page 7 Replace damaged Thermistor gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS 2 OVERFLOW test is flashing and audible alarm is present NOTE This is an optional function additional equipment is required If overflow alarm is activated power to the solenoid valve is disabled ALARM LN2 OVERFLOW Liquid Nitrogen has flooded vessel through internal vents V Series units only No lights Liquid level display showing incorrect reading Remote alarm working improperly or not at all www custombiogenics com CAUSES Liquid level has become too high inside the vessel walls because of stuck solenoid user error etc Disconnected sensor tube see 7 on page 7 EH settings are enable on a non EH unit High pressure supply Liquid level has become too high inside the vessel walls because of stuck solenoid user error etc
43. ne 90 of the display text The ALARM will flash on and off second intervals Press this button to Press this button to LOW ALARM is shown only as an example of reset alarm If it re mute audible alarm the alarm display window See page 13 for a repeat until all and advance to complete list of alarms alarms are corrected display 91 see below This display will appear when an 91 alarm condition is occurring AND the audible alarm is muted LOW ALARM is shown only as an example of the alarm display window See page 13 for a complete list of alarms Press this button to Press this button to reset alarm If it re enable audible alarm occurs repeat until all and return to display alarms are corrected 90 see above This display will appear during normal 499 operation when REPORT is selected from displays 1 2 or 3 Press button Press button Press REM button and to view alarms toview dataand the display will go back advance to display advance to display to whatever screen was 99 page 24 499A page 24 showing when the report was requested www custombiogenics com 15 CBS LCD DISPLAY FUNCTIONS Program Mode Display Windows This display will appear when the 4 PROGRAM LOCK switch is turned to PROGRAM Press this button to Press this button to Press this button to Press this button to advance to display ad
44. nges return to display 4 HI SET LOW SET return to display 4 MAIN MENU MAIN MENU see page 16 see page 16 This display will appear when TEMP 19 is selected on display 5 Use this menu to select temperature preferences i e F or C Press this button to Press this button to Press this button to select degrees select degrees discard changes and Fahrenheit and Celsius and advance return to display 4 advance to display 20 to display 20 MAIN MENU see page 19 see page 19 see page 16 www custombiogenics com 18 CBS LCD DISPLAY FUNCTIONS Program Mode Display Windows These arrows ve value Press button to Press button to save changesand discard changes and advance to display return to display 4 21 see below MAIN MENU see page 16 These arrows adjust settings 7 Press this button to Press this button to save the setting and discard changes and Return to display return to display 4 4 Main Menu MAIN MENU see page 16 see page 16 These arrows adj Press this button to Press this button to save changes and discard changes advance to display return to display 5 23 below MAIN MENU 2 see page 16 These arrows adj Ca value Press this button to Press this button to Press this button to print report instantly save changesand discard changes and return
45. o display 6 78 below Main Menu 3 see page 16 78 Press this button to Press this button to Press this button to Press this button to select a 24 hour time selecta 48 hour time selecta 72 discard changes and interval and advance interval and advance time intervaland return to display 6 to display 7 below to display 7 below advance to display MAIN MENU 3 7 below see page 16 Press this button to Press this button to Press this button to advance to display move the curser advance to display 16 Main Menu 3 between 6 MAIN MENU 3 see page 16 Hours and Minutes see page 16 8 o Press this button to Press this button to Press this button to move cursor left move cursor right return to display 4 MAIN MENU see page 16 www custombiogenics com 17 These arrows adj This display will appear when FILL TIMER is selected on display 6 This is a prompt to ENABLE or DISABLE the Automatic Fill Timer The Fill Timer gives the option of an automatic fill every 24 48 or 72 hours at a specified time of day These settings are programmed in the 2 menus that follow This display will appear when ENABLE is selected on display Use this menu to set the time interval that the automatic fill will occur This ese This display will appear will appear when any time interval is selected on display 78 Use this menu to set the time of day t
46. on the report display or printout Press this button to Press this button to return to whatever discard changes and screen was showing return to display 4 when the report was MAIN MENU requested see page 16 99C jJ Press this button to Press this button to return to whatever save the setting and screen was showing Return to display 4 when the report was Main Menu requested see page 16 www custombiogenics com This display will appear when enter is selected on display 99 see above Use this menu to choose whether you want the report to be printed or displayed on the screen COC BS LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to return to whatever screen was showing when the report was requested Press this button to scroll to the previous recorded event Press this button to scoll to the next recorded even C OV this to save the setting and Return to display 4 www custombiogenics com Press this button to save changes and advance to screen 47 below Main Menu see page 16 Press this button to advance to screen 47 below Press this to discard changes and return to display 4 MAIN MENU see page 16 This display will appear when DISPLAY is selected on display 99 see page 24 Use this menu to view either the ALARMS or DATA that was selected on screen 99 see 15 This display w
47. ondary or Back Up Alarm A Your freezer is a mechanical device and could malfunction By having a secondary or back up alarm you can minimize the possibility of a loss due to a malfunction Remote Alarm Connection to your Delta Room Facility Monitoring Station or Remote Auto Dialer Why should use my remote alarm feature or connection A If an alarm occurs after hours on a weekend or holiday the remote alarm connection will alert you to a problem and let you address it quickly Q lam not sure how to connect the alarm or what to connect the alarm to A First contact your lab manager supervisor or in house biotech representative If no one is sure what to do contact CBS customer service for help and direction What is an Auto Dialer and why would 1 use one A An Auto Dialer is used when a central connection is not available for the remote alarm When connected to a telephone land line the Auto Dialer will dial up to 8 different contacts If you have any question comments or concerns please contact Custom BioGenic Systems Customer Service Phone 586 331 2600 Toll free US only 800 523 0072 Email sales custombiogenics com www custombiogenics com 1 C E L5 SAFEI Y IMPORTANT The following section on SAFETY should be read carefully and followed completely Warnings regarding the handling of liquid nitrogen are by no means a complete volume on the safe use of cryogenic liq uids All personnel should have comple
48. priate PPE includes e Cryo gloves elbow length and loose fitting e Safety goggles full face shield Long sleeve lab coat e Cryo apron Long pants that come over the top of shoes e Fully enclosed footwear www custombiogenics com 9 C E L5 SAFETY Boiling Splashing and Spurting Introducing equipment which is at room temperature into liquid nitrogen is always somewhat hazardous Beware of splashing and boiling which may occur Never dip a hollow tube into liquid nitrogen it may spurt liquid nitrogen Compatibility Liquid nitrogen should ony be handled and transported in approved containers Liquid nitrogen may alter the physical characteristics of many materials make them brittle and fail Liquid nitrogen should never be used in combination with other substances or materials without knowing what the result will be When in doubt contact a competent authority Fire in Oxygen Enriched Atmostphere Liquid Nitrogen can condense oxygen from the atmosphere Liquid oxygen creates potential ly flammable or explosive conditions by greatly increasing the combustibility of many materials e g solvents hydrocarbons FIRST AID In the event a person has frostbite from liquid nitrogen the following first aid treatment should be given while awaiting the arrival of medics or a doctor 1 If the material has contaced skin or eyes flood those areas with large quantities of unheated water and protect frozen areas w
49. selected on display 6 This menu begins the testing of the overlay buttons Select BEGIN to C C proceed or SKIP to advance to the next window of the validation process Press this button to Press this button to begin testing the skip test and overlay buttons and advance to display advance to display 41 see below 41 and begin the final test see below This This display will appear when SKIP is will appear when SKIP is selected on display 40 or upon completion of testing the overlay buttons This menu tests the audible alarm This is the first in a series of test menus which will appear sequence when PASS or SKIP is selected Press this button to Press this button if Press this button if Press this button to test audible alarm test passes to test fails to advance skip test advance for each function being tested Buzzer should sound advance to display to display to display 441A 141A and next test and next test and next test below below below This display will appear when PASS FAIL or SKIP is selected on display 40 This menu tests remote alarm contacts Controller advances next test when PASS or SKIP is selected Press this button to Press this button if Press this button if Press this button to toggle remote alarm test passes to test passes to skip test advance contacts
50. t cover defects on cryogenic equipment resulting from abusive handling and subsequent structural failure To register you Custom BioGenic Systems product s please go to www CustomBioGenics com registration html Serial Number Model number For Technical Assistance Call 1 800 523 0072 U S Only Phone 586 331 2600 Fax 586 331 2588 www custombiogenics com amp t www custombiogenics com C_ RB Ss lt TABLE OF CONTENTS O amp A Liquid Nitrogen Safety Product Description Initial Set Up amp Operation Front Panel Controls ALL 2301 Carousel Side Panel V Series Manual Fill Instructions Feature Details Remote Alarm Wiring Storage Unit Parameters LCD Display Functions Standard Operating Mode Programming Mode Verification amp Calibration High amp Low Alarm Set Point Verification Temperature Display Verification 2301 Temperature Calibration V Series Level Display Verification amp Calibration Trouble Shooting Plumbing Wiring Diagram Part I D Plumbing Assembly Diagram Part I D 2301 Side Panel Diagram Part I D 2301 Controller Wiring Diagram Part 1 0 2301 Controller Wiring Diagram Part 1 0 2301 Controller Diagram Part I D 3000 Carousel Tank Diagram Part I D Cleaning amp Maintenance EMC Test Report www custombiogenics com Page 1 2 3 4 9 6 7 8 9 10 11 12 12 13 14 15 16 25 26 26 27 28 29 33 34 35 36 37 38 39 40 41 42 gt E lt gt RECOMMENDED BEST PRACTICES Recommende
51. te knowledge of the correct procedures as well as the hazards of work ing with liquid nitrogen Failure to do so could result in serious injury or death Be sure to read and understand the Liquid Nitrogen Safety Data Sheet provided by your Liquid Nitrogen Supplier WARNING To avoid risk of electric shock this equipment must only be connected to a supply mains with protective earth To prevent a rolling hazard ensure the casters are locked HAZARDS ASSOCIATED WITH LIQUID NITROGEN Asphyxiation When liquid is released into a confined space it displaces oxygen from the air This results in an oxygen deficient atmosphere particularly if vented into a closed space and asphyxia can result Entering an oxygen deficient room can cause unconsciousness without warning Always check air quality upon entering a room where cryogenic liquids are being used and if possible have an air respirator available Cold Burns Due to its extremely low temperature the cold vapor and gas that are produced when liquid nitrogen boils can give rise to cold burns or frostbite upon contact with unprotected skin Exposure may also produce discomfort in breathing and can provoke an asthma attack in susceptible individuals Never allow direct skin contact with liquid nitrogen or serious frostbite will result Personal Protective Equipment PPE must be used based on the principle that it should prevent liquid nitrogen from touching the skin and eyes Appro
52. tems at 1 800 523 0072 www custombiogenics com or email sales custombiogenics com www custombiogenics com CES IMPORTANT INFORMATION We at Custom BioGenic Systems are proud of our work and appreciate your purchase of this product With proper care this equipment will be trouble free for many years to come Before setting up and using your new cryogenic storage unit first check to see that all parts are accounted for and that no damage has occurred during shipping Also read this manual completely before proceeding to set up If at any time you are unsure of the procedures for set up and use of this product please contact CBS or your CBS sales representative NOTE If equipment is used in a manner not specified by Custom BioGenic Systems the protection provided by the equipment will be impaired PRODUCT WARRANTY Custom BioGenic Systems warrants all manufactured cryogenic equipment to be free from defects in workmanship or materials for a period of five year vacuum warranty and a 2 year warranty on electronics and parts Custom BioGenic Systems liabilities under the warranty shall be limited to correcting or replacing defective workmanship or materials A claimant under the warranty must notify Custom BioGenic Systems within 10 days after the discovery of the defect Custom BioGenic Systems reserves the right at their discretion to correct the defect s in the field without return shipment to the factory This warranty does no
53. the 0 5V outputs NOTE Outputs are for use with an external monitoring system through the 16 port connector This display will appear when LOG is selected on display 7 see page 16 This menu is used to choose how often you wish the data Temp A Temp B and Level to be recorded The recorded info can be displayed printed when the report function is selected from screens 1 2 or 3 COC BS LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button to Press this button to Press this button to disable OFAF and select Simultaneous select Sequential discard changes return to display and Advance to and Advanceto return to display 74 MAIN MENU screen 46 screen 46 MAIN MENU see page 16 see page 25 see page 25 see page 16 Press this button to return to whatever screen was showing when the report was requested Press this button to discard changes and return to display 4 MAIN MENU see page 16 This display will appear when OFAF is selected on display 7 see page 16 This display will appear when either alarms or data is selected on display 99 see page 15 Use this menu to select the start date that you want the alarm data to begin on the report display or printout This display will appear when enter is selected on display 499A see above Use this menu to select the end date that you want the alarm data to end
54. vance to display advance to display advance to display 8 DATE TIME 13 TANK ID 15 LIQUID LEVEL 5 MAIN MENU 2 settings settings settings see below see page 17 see page 17 see page 18 This display will appear when NEXT is 5 selected on display 4 Press this button to Press this button to Press this button to Press this button to advance to display advance to display advance to display advance to display 19 LID TEMP 22 24 55 6 MAIN MENU 3 settings settings settings see below see page 18 see page 19 see page 19 This display will appear when NEXT is 6 selected on display 5 Press this button to Press this button to Press this button to Press this button to advance to display advance to display advance to display return to display 40 VALIDATION 7 FILL TIMER 42 REVISION 4 MAIN MENU settings settings parameters see above see page 19 see below see page 22 This display will appear when NEXT is selected on display 6 7 ss this button to Press this button to Press this button to advance to advance to screen operation and Display 44 45 see page 25 return to display 4 see page 24 MAIN MENU see above www custombiogenics com 16 CB Ss LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button to ENABLE Fill Timer DISABLE Fill Timer this willadvance this will return you you to display t
55. ways override the Fill Timer setting Lid Temperature Display Lid Temperature Thermocouple is located approximately 11 from the top of the tank Lid Temperature Thermocouple is located approximately 11 from the top of the tank 0 5Vdc amp 4 20mA Outputs Easy screw terminal output connections for existing alarm and monitoring systems This feature is available through the Tank ID menu See page 16 screen 4 Select either 0 5Vdc with connections to Temp A Temp B and Level 4 20mA with connections to Temp A Temp B and Level Fill Vent In Progress Signal to activate the Custom BioGenic Systems TS 1B LN2 supply tank switcher or an additional 24Vdc valve to control the LN2 supply Report Log Feature See page 15 screen 99 Alarms Records Sequence of events such as POWER etc Data The LOG function is enabled for use with either Cryomonitor or Reporting This will Date Time stamp Temp A Temp B and Level at the programmable rate of 1 90 hours on the hour This information is selected by date range to either be displayed or printed for easy review Optional Features Overflow Sensor Not included as standard equipment can be purchased separately This feature uses a thermistor set at the bottom of the storage space along the probe holder tube If an overflow occurs it will cause the LN2 to make contact with the thermistor An audible and visual alarm will occur and SYSTEM STATUS ALARM LN2 OV

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