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ABX Micros CRP 200

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3. 289 ErP14 25 R16 2 e Pm lt 16 NEN Threshold Threshold Aperture Threshold Nollage 4 M Fan RF p34 Gain 11 15 28 PLT Pulse Process RBC Pulse Process WBC Pulse Process RS232 J22 p TP2 TP1 Supplies CN Em Em Lain DS6 TP20 IPs EEE DS3 22 Hil DS4 TP23 2 DS5 TP21 M vs16 CRP Printer J31 J5 board B Liquid valves 8 2 14 15 8 9 5v E A ES hi A ae Power ES ax ES Ground 4 KE Ni 1 1 lt Vacuum Syringe Reagent Syringe Carriage CRP Syringe 24V 12V 12V Cycle start pa F gt r 2 Geo Piercer a Sensor Motor Sensor Motor Sensor Motor Sensor 21 VACUUM SYRINGE REAGENT SYRINGE CARRIAGE CRP SYRINGE Fig 1 Mother board RAS262 2 ABX Micros 200 Technical Manual RAAO28A Micros CRP 200 2 WBC RBC PIT thresholds adjustment 2 1 WBC Threshold Adjustment Target 280 mV 5 Remove the MICROS CRP cover Switch on instrument Check between TP10 and TP31 Ground WBC threshold If necessary adjust WBC threshold by R68 potentiometer on mother board See Fig 1 Mother board page 2 2 2 RBC Threshold Adjustment Target 400 mV 5 Remove the MICROS CRP cover Switch on instrument Check between TP13 and TP31 Ground RBC threshold If necessary adjust RBC threshold by R75
4. 8 3 ABX Micros CRP 200 Technical Manual RAA028A 8 1 ABX Micros CRP 200 1 Maintenance 1 1 Introduction having attended a HORIBA ABX approved customer training course The system warranty may be affected if damage occurs after a non trained technician intervenes or if replaced spare parts and consumables do not come from a HORIBA ABX approved origin Customer maintenance has to be carried out according to the recommended frequency after 1 2 Daily customer maintenance Only a Startup and a Shutdown are required at the beginning and the end of the day Those cycles are described in the User manual Section 4 Startup amp sample analysis 1 3 Weekly customer maintenance An overall check for cleanliness of the system is recommended every week Any traces of blood or reagent have to be wiped off as soon as possible using a piece of cloth and distilled water Never use solvent or abrasive cleaning material to clean the system 1 4 Acces to the Technician functions In order to access to the special functions follow the procedure below Check that the instrument is in ready mode Press and hold the Service key blue Using the Up and Down keys set up the ten number of the required function on the WBC display then press Enter Keep holding the blue Service key and set up the unit number using the Up and Down keys Press Enter when the required unit number is selected Release the blue Servi
5. 3 6 ABX Micros 200 Technical Manual RAA028A 3 1 ABX Micros CRP 200 1 Hydraulic 1 1 Hydropneumatic connections Circuit Diluent reagent connection Liquid sensor 1 T1 1 EV11 3 Temperature sensor EV11 2 EV10 2 T2 3 Sample syringe 2 T2 2 EV15 3 EV15 1 EV14 1 EV14 2 T4 1 CRP flowcell bottom EV10 1 EV7 1 Rinsing block 1 EV7 2 T5 2 WBC chamber 2 RBC chamber 2 Lyse bottle Liquid sensor 2 EV1 3 EV1 2 Liquid earth T3 2 Cleaner bottle Liquid sensor 1 T1 3 EV11 1 Temperature sensor Diluent syringe 3 EV10 3 T2 1 Sample syringe 5 Sample needle EV15 2 CRP syringe 1 EV14 3 T1 2 T4 2 CRP flowcell top CRP mixing chamber EV7 3 Rinsing block 2 EV8 3 T6 1 WBC chamber 3 RBC chamber 3 EV6 2 Liquid sensor 2 EV1 1 Lyse syringe 4 Liquid earth 13 3 WBC chamber 1 Liquid sensor 2 ABX Micros CRP 200 Technical Manual RAA028A Hydraulic amp Pneumatic principles Hydraulic Liquid sensor 2 EV4 2 EV4 1 T5 3 ss Ss Air syringe 1 EV2 1 EV2 2 Air Air syringe 2 Pressure sensor on CRP board Air syringe 3 EV8 1 Air syringe 4 EV6 1 7 EA IZ GENE XS Air syringe 5 T6 1 T6 3 EV5 2 EV5 1 Waste connection T6 2 7 1 7 3 EV18 1 EV18 2 T4 3 T7 2 T8 3 T8 2 EV12 1 EV12 2 Isolator W Isolator W T3 1 T8 1 EV13 1 EV13 2 Isolator R Isolator R RBC chamber 1 ABX Micros 200 Technical Manual RAA028A 3 3 ABX M
6. Includes Startup Pause Mode and Shut down keys and 7 LCD displays Connected to CRP board via flat connector in J1 Keyboard board flat connector is connected in J2 Power supply is connected in J3 4 Switch board LIII O os 2 77 e SWS 567 4 503 SWe 1 i 1 I I E Le 2 rp 4 ccc 4 loo 4 poe sem 1 o I Rove 1 acd 1 I 1 I I sho Sk4 541 SWB L L L I L Les ar De c Support keyboard and is connected to Display board 4 6 ABX Micros 200 Technical Manual RAA028A Electric amp electronic principles Connections 5 Connections 5 1 Flat cables E1000349200 J1 on Switch board J2 on Display board E1000349100 CN3 on CRP board J1 on Display board E1000348900 J1 on liq valve block 8 10 235 on Main board H1009392001 J31 on Main board Printer output H1009391001 J22 on Main board RS232 output E1000349000 CN1 on CRP board J1 on liq valve block 5 18 6 Synoptic See Synoptic on next page ABX Micros CRP 200 Technical Manual RAA028A 4 7 ABX Micros CRP 200 4 8 ABX Micros CRP 200 Technical Manual 28 pyiverson VScEOLOLA kee hee 329342
7. CSNIFA FINE JD 52012 O O 20 O o Do O O OO canten pinejap T BOOK 5340 casn auning 33832 O iuo f El jumper configuration language setting 1 2 Main Board configuration 4 4 ABX Micros CRP 200 Electric amp electronic principles CRP board general view 2 CRP board general view Needle carriage Battery Sensor Motor CN2 CN6 Display board CRP blank adjustment CRP ac jal measurement unit VR1 Pressure sensor e Reagent yal cooling unit TP16 D lt a ressure sensor 2 adjustment Mother board Liquid valves 145 18 12 7 4 9 13 5 TP6 mu CN10 CNTS CN CNS Security Power Diluent Cleaner Lyse Mixing chamber Magnet Supply Sensor Sensor Sensor Reagent door 2 detection The CRP board drives all CRP functionnalities CRP chamber heating CRP reagents cooling CRP reagent door detection CRP measurement CRP board is connected to main board via CN4 connector Display board is connected to CRP board via CN3 connector Power supply board is connected via CN5 connector 2 1 Test points Adjustment Test point Potentiometer Target value Pressure sensor 16 on board TP6 CRP board VR2 on CRP board 1 7V 0 1 ABX Micros CRP 200 Technical Manual RAA028A 4 5 ABX Micros CRP 200 3 Display board E 0 El E
8. sepes Monocytes Granulocytes Pulse height Cells passing through the WBC aperture creating electronic pulses 5 2 ABX Micros CRP 200 Technical Manual RAA028A Analysis cycle technology Measuring principles Number of cells Cells are grouped according to the number of cells and the cell size Cell volume Pulses are electronically calculated and smoothed to produce the WBC odi distribution curve Results Number of cells counted per volume unit x calibration coefficient Histograms Distribution curves on 256 counting channels from 30fl to 450fl Cell volume Technical characteristics for the WBC count Dilution Initial blood volume 10 ul Vol ABX DILUENT 2500 pl First dilution rate 1 250 Vol Lyse 520 Second dilution rate 1 300 Measurement Method Impedance Ruby diameter 80um Depression of count 200mb Duration of the count 2x5 sec ABX Micros CRP 200 Technical Manual RAAQ28A 5 3 ABX Micros CRP 200 1 2 RBC PLT The RBC s and PLT s are measured by an electronic impedance variation principle This means that an electronic field is generated around the micro aperture within the chamber in which the blood cells are pulled through The sample is diluted with an electrolytic Diluent electronic current conducting fluid mixed then pulled through a calibrated micro aperture Two electrodes are placed on either side of the aperture and electric current continuously pass
9. 200 Technical Manual RAAO28A Micros 200 3 WBC and RBC chambers maintenance 3 1 RBC chamber Counting head electrode seals replacement 1 x GBG275A 1 x FAA046A Run a drain chamber cycle F 12 Remove the chamber cover See Fig 6 Chamber cover page 57 Fig 6 Chamber cover Record the tube positions before dismantling the chambers then unclip the RBC chamber See Fig 7 RBC chamber page 5 Fig 7 RBC chamber Dismantle the electrode 6 loosening the 2 fixation screws 7 and the terminal holding screw 3 See Fig 8 RBC chamber exploded view page 6 ABX Micros 200 Technical Manual RAAO28A RAS258 5 RAS258C Fig 8 RBC chamber exploded view Use a previously cut micropipette tip to replace the electrode O ring 1x FAA046A as shown on following diagram Install the chamber over a piece of white paper or cloth Carefully remove the counting head 4 and plunge the aperture 5 in distilled water Do not manipulate the aperture using hard instruments Clean the aperture with a piece of soft paper or preferably in between 2 fingers Replace the aperture seal 2 GBG275A Rinse thoroughly with distilled water Dry the exterior of the chamber with a soft paper A Do not apply too much pressure on the electrode fixation screws as it can break the aperture tightening torque 100mN m 14 2 Ozf in It is recommended to reconnect the tubes on
10. 5 Needle and carriage location over CRP chamber adjustment page 6 RAS261 Enter CRP needle value See 5 Needle and carriage location over CRP chamber adjustment page 6 RAS261 Enter CRP carriage value See 5 Needle and carriage location over CRP chamber adjustment page 6 RAS261 CRP R2 needle adjust See 6 Needle and carriage location over CRP R2 reagent chamber adjustment page 7 RAS261 Enter CRP R2 needle value See 6 Needle and carriage location over CRP R2 reagent chamber adjustment page 7 RAS261 Carriage holder adjust See 1 Carriage sampling location adjustment page 2 RAS261 Enter carriage holder value See 1 Carriage sampling location adjustment page 2 RAS261 Position check of the needle Up Down and Backward Forward over the WBC chamber Set the needle tip position to the one Value thirds position from the right side of the WBC chamber and press the Enter key The Backward Forward position is automatically adjusted 1000 Units Enter the pulse count of the needle Backward Forward over the WBC chamber Needle value Locate the tip of the needle to the right side of the CRP mixing chamber and press the Enter key The Backward Forward position is automatically adjusted Position check of needle Backward Forward over the CRP mixing chamber 1000 Units Enter the pulse count of needle Up Down over the CRP mixing chamber 1000 Units Enter the pulse count of the
11. ABX Micros 200 Technical Manual RAA028A 2 3 ABX Micros CRP 200 3 ABX Micros CRP 200 description 3 1 Front view MICPOS Start Pause key 4 E This key is used to change the sleep state to the measurement ready state Y It is also used to set the instrument in the sleep state Press this key to cancel an error Mode Key This key is used to toggle the measurement mode among Blood count measurement item mode CBC CBC measurement mode QC CBC and QC CRP check quality assurance This key is also used to open the sample holder in the sleep state Shut Down key Press this key when ending measurement for the day pressing this key performs automatic q r cleaning and fills the instrument with the cleaning liquid The power switch can be turned OFF in this state This key is used to start measurement This holder holds the sample tube and the control blood measurement starts by closing the holder O Measurement key amp sample holder 2 4 ABX Micros 200 Technical Manual RAAO28A Specifications ABX Micros CRP 200 description Reagent door 1 open close Allows the access to reagents by pushing the place where PUSH is written Display unit Displays the measurement results and operating state of the instrument 3 2 Display unit dy CYCLE M READY E zx BUSY O 47 Error Cycle LED Lights up or blinks during meas
12. See Fig 1 Mother board page 2 5 3 Power Supply 24V Target check 24V 2 1 Remove the MICROS CRP cover Switch on instrument Check between TP22 and TP31 Ground 24V Power suply voltage See Fig 1 Mother board page 2 5 4 Power Supply 5V Target check 5V 0 15 Remove the MICROS CRP cover Switch on instrument Check between TP23 and TP31 Ground 5V Power suply voltage See Fig 1 Mother board page 2 6 Aperture voltage check Target check 60V 1 5 2 8 Remove the MICROS CRP cover Switch on instrument then enter F 63 function Check between TP19 and TP31 Ground 60V Aperture voltage See Fig 1 Mother board page 2 ABX Micros 200 Technical Manual RAAO28A 7 CRP blank adjustment Run a diluent priming F 13 function Micros 200 Enter F 91 function Photometer adjustment The instrument automatically fills the flow cell with diluent and shows the CRP blank value on WBC display Perform F 91 five times in order to check the repetability of values and verify that the value does not fall more than 10 within 30 seconds By means of VR1 on CRP board See Fig 3 CRP board page 7 adjust displayed value to 3800 10 A The adjustable range of VR1 is 270 degrees Attempting to turn it in excess will damage VR1 Needle carriage Battery Sensor Motor CN
13. See Fig 1 Mother board page 2 adjust the HGB channel according to the room temperature using the following chart table Tab RAS262 1 HGB channel Channel Temperature room Nominal 15 240 245 250 16 240 245 250 17 239 244 249 18 238 243 248 19 237 242 247 20 236 241 246 2d 235 240 245 22 234 239 244 RAS262 8 ABX Micros 200 Technical Manual RAAO28A Micros 200 Channel Temperature room Nominal 24 233 238 243 25 232 237 242 26 231 236 241 27 230 235 240 28 229 234 239 29 228 233 238 30 228 233 238 31 227 232 237 32 226 231 236 33 225 230 235 34 224 229 234 35 223 228 233 9 WBC latex adjustment A As the WBC gain is a factory adjustment it is mandatory not to readjust it without taking the following precautions Carry out previously an autoconcentrated cleaning to make sure of the cleanliness of the WBC counting circuit If necessary clean the WBC chamber aperture Do not operate gain adjustment as long as the lympho and granulo values are not stable Make sure the Latex has been thoroughly mixed before Remove the MICROS CRP cover Switch on instrument Run F 64 function WBC Latex Adjust This function allows the dilution of latex in diluent and displays Lymphocyte and Granulocyte volumes on screen every 3 seconds Value blinking in WBC display shows the LYM target depending on the Latex lot used usually 52 1 if necessary you can change this value by
14. function enter previous Value 20steps for the vial bottom thickness then press Enter Enter F 82 function to check correct adjustment with the empty vial Press Enter the needle moves down Manually move the needle it must not touch the bottom of the vial Press Enter the needle goes up and the tube holder opens Repeat these operations until the adjustment is correct ABX Micros 200 Technical Manual RAAO28A RAS261 9 RAS261C RAS261 10 ABX Micros 200 Technical Manual RAA028A Micros 200 5262 Main board amp board Concerns Mother board and CRP board adjustment amp check Required tools Hexagonal keys Voltmeter Flat screwdriver Required products WBC Latex RBC PLT Latex ntervention time 1 hour Frequency On request Specific kit or consumables e WBC Latex LADOO1DS RBC PLT Latex LADOO2BS Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS262C 1 Mother board general view O HGB J38 J39 ETP31 ETP30 29 assembly J21 Temperature omg O di ade is sensor rt RBC Coax WBC Coax HGB m m TP32 D wi il pee il
15. or after a specific operational function Graphics All graphics including screens and printouts photographs are for illustration purposes only and are not contractual Trademarks Other product names mentioned within this publication may be registered trademarks of other companies Copyright 9 2005 by HORIBA ABX All rights reserved No part of this book may be reproduced or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without the prior written permission of HORIBA ABX HORIBA ABX Rue du Caduc e Parc Eurom decine 34184 MONTPELLIER Cedex 4 FRANCE Tel 33 0 4 67 14 15 16 Fax 33 0 4 67 14 15 17 ABX Micros CRP 200 Technical Manual RAA028A Introduction Working conditions 1 Working conditions 1 1 Environment The operation of the ABX Micros CRP 200 should be restricted to indoor location use only Operation of the instrument at altitudes of over 2000 meters 6000 feet is not recommended The instrument is designed for safety from voltage surges according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2 IEC EN 61010 1 Please contact your local HORIBA ABX representative for any information regarding the operation location when it does not comply with the recommended specifications 1 2 Location The ABX Micros CRP 200 should be placed on a clean and leveled table or workbench Please note that the ABX Micros CRP 200
16. 1 Check solenoid Remove any physical obstruction Check switches connections 2 Tighten or loose the push screw located behind the tube holder Adjust the spring position Run a Startup cycle ABX Micros CRP 200 Technical Manual RAAO28A Error Cause code E20 E22 E23 E24 E25 E26 E27 A28 A29 A30 The instrument is waiting for printing to end Diluent temperature sensor is disconnected or broken Diluent temperature sensor is broken HGB blank value exceeds the specified value Heater does not turn on off in the specified time Heater does not turn on off in the specified time CRP blank value exceed the specified value Diluent sensor Lyse sensor Cleaner sensor Alarm amp error list Measurement has been started though paper runs out during printout Diluent temperature sensor is disconnected or open circuit is present Diluent temperature sensor is defective HGB blank value is not within the range between 180 and 255 though measured up to 3 times or gap from previous value is more than 5 Temperature is low Wire of the heater is broken Temperature is high Cooler is in failure CRP blank value is not within the range between 3340 and 4014 Air bubbles in the flowcell Low intensity of CRP light source LED Too high needle position in mixing chamber may causes air bubbles during priming Diluent is not primed Connector is disconnected Lyse
17. 1 EACO10A FITTING LUER FEMALE I 3MM 2 F1000380800 FITTING ANTI ROTATION WASHER 3 G0166210 REAGENT CONNEXION PLATE CRP200 ABX Micros 200 Technical Manual RAA028A 9 17 ABX Micros CRP 200 14 Front view 1 XBA281A SENSOR TEMPERATURE MIC LC220 2 E1000117600 PROTECTION SHIELD FOAM L 1M 3 008 SYRINGE PLASTIC GUIDE L 63 5 4 DBE018A CABLE BUSHING D 9 5 BLACK 5 DBE017A CABLE BUSHING D 23 8 BLACK 6 DBE021A CABLE BUSHING D 12 7 BLACK 7 589 5 VALVE 7 110 VALVE ASSY 10 8 8 F1000386000 CUP OVERFLOW SYRINGES CRP200 9 18 ABX Micros 200 Technical Manual RAA028A Exploded views 15 Display board view 04148 085 IND B 1 E0013464600 PCB DISPLAY BOARD CRP200 ABX Micros 200 Technical Manual RAA028A 9 19 ABX Micros CRP 200 16 Syringe mechanical view 1 G0166330 G0167040 H1008310001 H1008311001 H1008333002 H1008357001 H1008359001 H1008376001 G2002534 G2002535 H1001636001 H1008338002 ON DN HR W PP 9 20 MOTOR STEP BY STEP GEAR CRP200 SENSOR OPTIC CRP DIL SYR CRP200 SYRINGE PULLEY SYR LIQ AIR CRP200 SYRINGE WASHER SYR LIQ AIR CRP200 MOTOR CROSSPIECE S LIQ AIR CRP200 SYRINGE GEARING S LIQ AIR CRP200 SYRINGE COGGRAIL FOR LIQ SYR CRP200 MOTOR REDUCTOR PLATE ASSY CRP200 MOTOR REDUCTOR 3 SYR CRP200 MOTOR REDUCTOR CRP SYR CRP200 SYRINGE 3 SYR TRANS GUIDE CRP200 SYRINGE CRP TRANS GUIDE CRP200 ABX Micros CRP 200 Technica
18. 10 MCHC Mean Corpuscular Hemoglobin Concentration is calculated according to the HGB and values The Mean HGB concentration in the total volume of RBC is given by the formula MCHC g dL HGB HCT x 100 1 7 Measuring the MPV MPV Mean Platelet Volume is directly derived from the analysis of the platelet distribution curve The MPV is expressed in um3 or fl 1 8 Calculating the PCT Thrombocrit is calculated according to the following formula PLT 10 uL x MPV fL 1000 PCT 1 9 Calculating the PDW This count is derived from the platelet curve PDW Platelet Distribution Curve Width of the curve between 15 of the number of platelets starting from 2 fl S1 and 15 of the number of platelets beginning with the variable top threshold S2 as shown on next diagram 5 6 ABX Micros CRP 200 Technical Manual RAA028A Analysis cycle technology Measuring principles 1 10 CRP measuring principles 1 10 1 CRP count principles Measurement Same as the hemoglobin measurement principle it is a spectrophotometric absorbance measurement Turbidimetry of the C Reactive protein after 2 dilutions This measurement is performed in CRP Chamber Technical characteristics for the CRP count Dilution 1 Initial blood volume 8 ul Vol ABX CRP REA R1 Saponin 100 yl Fisrt dilution rate 8 100 Total time for reaction 60 sec Dilution 2 Dilution 1 108 ul Vol ABX CRP REA R2 Buffer 100 ul
19. 9 12 ABX Micros CRP 200 Technical Manual RAA028A xploded views 9 RBC chamber view OA Ui W BP XBA413A FAA046A XDA470ES GBG275A GBC239A H0525166003 F2000037700 CABLE COAX RBC MICROS 60 O RING COAXIAL CABLE CHAMBER RBC COMPLETE O RING APERTURE D 0 5 P60 P80 CHAMBER COUNTING HEAD MIC 60 CHAMBER APERTURE 50 CHAMBER RBC BODY CRP200 ABX Micros 200 Technical Manual RAA028A 9 13 ABX Micros CRP 200 10 WBC chamber view 1 XBA412A CABLE COAX WBC MICROS 60 2 XDA472B CHAMBER HB ASSY MICROS 60 3 XDA471ES CHAMBER WBC HB MICROS 60 CPTE 4 H0525166002 CHAMBER APERTURE 80 5 GBG275A O RING APERTURE D 0 5 P60 P80 6 GBC239A CHAMBER COUNTING HEAD MIC 60 7 FAA046A O RING COAXIAL CABLE 9 14 ABX Micros CRP 200 Technical Manual RAA028A xploded views 11 Power supply view 1 DBMOO1A SWITCH POWER ON OFF 2 E1000342800 PCB POWER SUPPLY BOARD CRP200 3 DBK012A CLIP FAN HOLDER 4 DBK003A ADHESIVE HOLDER FOR FLAT CABLE 5 60166270 FAN POWER SUPPLY FAN CRP200 6 DBEO18A CABLE BUSHING D 9 5 BLACK ABX Micros CRP 200 Technical Manual RAA028A 9 15 ABX Micros CRP 200 12 Cooling fan view 1 2 3 4 G0172080 H1008391001 DBE018A 62002532 200 COVER REAR FAN PROTECTION CRP200 CABLE BUSHING D 9 5 BLACK FAN MAIN FAN FILTER ASSY CRP200 ABX Micros CRP 200 Technical Manual RAA028A Exploded views 13 Reagent connection plate view
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21. CELL LYSE DISP MIC 2 FAA053A O RING SAMPL NEEDLE MICROS OT 1 FAA055A O RING MICROS SAMPLING SYRINGE 2 GBG145A Reagent straw cap D 20 1 GBG155A Capsule D 25 1 GBC284A Cleaner straw 1 GAK302A Cap 1 XDA693A Waste tubing P60 1 JAG003A Plastic box 1 1 Bent key 2mm 1 002 Bent key 2 5mm 1 Bent key 1 5mm 1 018 Bent key 3mm 1 069 Screwdriver 2 5mm 1 MABO90A Torx bent key T10 1 019 Grease KM1011 1 G0166911 Diluent straw tube 1 G0166920 Waste straw for waste container 1 GBC285A Waste straw for waste plastic bag 1 G0310450 CRP reagent protection cover 1 G0166861 Waste water tank 1 G0166940 Sample cup 20 2 Printer Use the printer that is supplied or approved by HORIBA ABX Do not use any printer that has not been recommended by a HORIBA ABX qualified technician RAS256 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 3 Installation 3 1 Removing the fixture securing the carriage for transportation Fixture Open the reagent door 2 Move the fixture securing the carriage to the right Shift the fixture horizontally with your thumb and index finger Release the lock and remove the fixture EY Save the fixture since it is used for TRANSPORTATION Peel off the tape securing the reagent cooling unit and reagent cover open close When peeling off the tape be sure that the reagent protection cover between the reagent cooling
22. CRP 200 22 Main board view DBK014A CABLE HOLDER RS FLAT CABLE DBK015A CABLE HOLDER PRINT FLAT CABLE Not available at the time of publication Not available at the time of publication 9 26 ABX Micros 200 Technical Manual RAA028A xploded views 23 Front panel view Number Reference Designation 1 G0332140 COVER FRONT COVER ASSY CRP200 2 L0163761 COVER FRONT REAGENT DOOR CRP200 3 L0338230 COVER KEYBOARD CRP200 4 F0022990300 COVER REAGENT DOOR LOCK CRP200 ABX Micros CRP 200 Technical Manual RAA028A 9 27 ABX Micros CRP 200 24 Sample holder view 1 CAE006A SWITCH MICROSWITCH XC5 81 82 2 DAMOO6A VALVE SOLENOID MICROS CT PIERC 3 60166791 SAMPLING BLOCK ASSY CRP200 4 G0175390 SAMPLING STANDARD TUBE HOLDER 5 G0289970 SAMPLING BRAKING GEAR SPRING 6 H1016977001 COVER SAMPLING BLOCK DOOR CRP200 9 28 ABX Micros CRP 200 Technical Manual RAA028A xploded views 25 Cover view 1 F1001306800 COVER SIDE DOOR LOCK ASSY CRP2 2 G0166801 COVER MAIN COVER ASSY CRP200 3 H1031791001 COVER CRP REAGENT DOOR CRP200 ABX Micros 200 Technical Manual RAA028A 9 29 ABX Micros CRP 200 26 Back view Rewistration number im Japan 26820120 Product name AUTOMATIC BLOOD CELL COUNTER PLUS CRP 10 176 CRP 20503013 Date 2005 1 ACTOD 240N 15094 50 6002 Men WORIBA L td 2 Mivanch iash1 Kisthoin Minami kur Kyoto Jaran HORIBA TO AVOID ELECTRIC POWER CABL
23. Cutting pliers Required products Liquid soap Distilled water Scalpel Micropipette tip Silicone grease LAM 004 A Soft paper Grease for mechanical assemblies XEA381A Grease Oil Vactra XEA821A ntervention time 1h30min Frequency Once a year Specific kit or consumables Yearly maintenance kit XEA931AS Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS258C Yearly Maintenance Kit XEA931AS RAS258 2 FAA017A F0020373000 FAA036A FAA046A FAA053A FAA055A H1008304002 GBG275A 1 Vacuum syringe O ring replacement 1x FAA017A O RING TANK MIN AG WASTE MIC 15x1 5 Nit 70SH O ring O RING FLOW CELL LYSE DISP MIC O RING COAXIAL CABLE O RING SAMPL NEEDLE MICROS OT O RING MICROS SAMPLING SYRINGE Liquid Syringe Diluent Piston O RING APERTURE D 0 5 P60 P80 1 1 2 Swith off the instrument and disconnect the power supply cable Remove the cover Disconnect on vacuum syringe the fourth tube from the top of the syringe Unscrew the 4 screws A maintening the syringe body to the frame See Fig 1 Fourth tube page 2 Fig 1 Fourth tube Manually pull up the piston outside of the vacuum syringe body Unscrew the 4 screws from the top plate of the vacuum syringe See Fig 2 Syringe screws page 3 Remove the syringe
24. In order to access to the technician functions follow the procedure below Press and hold the Service key blue Using the Up and Down keys set up the ten number of the required function on the WBC display then press Enter Keep holding the Service key blue and setup the unit number using the up and down keys Press the Enter key when the required unit number is selected _ To access a function from F60 to F98 Press the Up and Down key while simultaneously pressing the Service key and the Mode key Release both keys F5 Technician 1 needle adjustment F51 Needle depth adjustment Locate the tip of the needle to the right Value See 3 Needle heigth side of the WBC chamber then press the adjustment page 4 RAS261 Enter key The Up Down position is automatically adjusted F52 Enter needle value Enter the pulse count of needle Up 1000 100 10 1 Down value over WBC chamber Units Units Units Units ABX Micros CRP 200 Technical Manual RAA028A 6 5 ABX Micros CRP 200 CE E CCC F53 F54 55 56 F57 F58 59 F5A F5B F5C F5D 6 6 Needle amp carriage check See 4 2 Adjustment Check page 5 RAS261 CBC carriage adjustment See 4 1 Adjustment page 5 RAS261 Enter CBC carriage value See 4 2 Adjustment Check page 5 RAS261 CBC amp CRP carriage adjust See 5 Needle and carriage location over CRP chamber adjustment page 6 RAS261 CBC amp CRP carriage check See
25. Power supply view page 15 11 Power supply view page 15 11 Power supply view page 15 11 Power supply view page 15 12 Cooling fan view page 16 12 Cooling fan view page 16 12 Cooling fan view page 16 12 Cooling fan view page 16 13 Reagent connection plate view page 17 13 Reagent connection plate view page 17 13 Reagent connection plate view page 17 14 Front view page 18 14 Front view page 18 14 Front view page 18 14 Front view page 18 14 Front view page 18 14 Front view page 18 14 Front view page 18 14 Front view page 18 15 Display board view page 19 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 16 Syringe mechanical view page 20 17 Carriage view page 21 17 Carriage view page 21 17 Carriage view page 21 OQ O KR 0 ON DY 0 FP UO P DY PN DY PF B gt F2000037700 XBA412A XDA472B XDA471ES H0525166002 GBG275A GBC239A FAA046A DBM001A E1000342800 DBK012A DBK003A G0166270 DBE018A G0172080 H1008391001 DBE018A 62002532 EACO10A F1000380800 G0166210 XBA281A E100011760
26. Valve 14 CRP syringe goes up CRP syringe goes down CRP syringe goes up Movement over WBC chamber and needle exterior and interior cleaning Sampling needle carriage initialization CRP reagent flap is closed Carriage goes right RAS260 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 CRP syringe goes down Sampling needle carriage goes down Valve 11 Liquid syringe goes up Valve 10 CRP syringe goes up WBC chamber and wastes draining Valve 12 Vacuum syringe goes up Liquid syringe goes down Vacuum syringe initialization Valve 5 Valve 11 Liquid syringe goes up 2 2 Preparation of CBC dilutions and counting Needle up and rinsing block cleaning carriage goes to sampling tube Sampling needle carriage initialization Liquid syringe initialization Valve 8 Vacuum syringe goes up Sampling needle carriage goes down Carriage initialization Liquid syringe goes down Valve 5 Vacuum syringe initialization Valve 2 4 10ul blood sampling for CBC count Needle carriage goes down Liquid syringe goes down Needle initialization and cleaning Vacuum syringe goes up Valve 11 Valve 8 Liquid syringe goes up Carriage initialization Sampling carriage goes up Sampling door is opened Carriage goes right Hgb blank measurement and counting heads cleaning Hbg blank calculation Va
27. and printer weigh approximately 30 kilograms 66 lbs Avoid direct exposure to sunlight Place your instrument where it is not exposed to water or vapor 4 Place your instrument where it is free from vibration or shock Place your instrument where an independent power receptacle can be used Use a receptacle different from the one used by a device that easily generates noise such as a centrifuge etc Proper ventilation is required on the ABX Micros CRP 200 Provide a space of at least 20 cm 8 inches at the back of the instrument to prevent overheating of the power supply The ON OFF switch and the input voltage connection must always be accessible When A positioning the system for operational use leave the required amount of space for easy accessibility to these items 1 3 Grounding Proper grounding is required when installing the system Check the wall outlet ground Earth for proper grounding to the facilities electrical ground If you are unsure of the outlet grounding contact your facilities engineer to verify the proper outlet ground 1 4 Electromagnetic Environment Check The ABX Micros CRP 200 has been designed to produce less than the accepted level of electromagnetic interference in order to operate in conformity with its destination allowing the correct operation of other instruments also in conformity with their destination In case of suspected electromagnetic noise check that the instrument has not
28. been placed in the proximity of electromagnetic fields or short wave emissions i e Radar X rays Scanners Cell phones etc ABX Micros 200 Technical Manual RAA028A 1 1 ABX Micros CRP 200 1 5 Humidity and temperature conditions Temperature range CBC mode and CRP mode CBC CRP ABX Micros CRP 200 must function between 18 to 30 C 65 to 86 F Results will be displayed under 18 64 4 F but are not reliable Humidity Maximum relative humidity should be 80 for temperatures up to 31 88 F decreasing linearly to 50 relative humidity at 40 C 104 F If the system is kept at a temperature less than 10 C 50 F or less it must be allowed to sit at room temperature for 1 hour before it can be used for operation 1 6 Environmental Protection Disposal Used accessories and consumables Must be collected by a laboratory specialized in elimination and recycling of this kind of material according to the local legislation Disposal ABX Micros CRP 200 instrument It should be disposed of in accordance with local legislation and should be treated as being contaminated with blood The appropriate biological precautions should be taken If any doubt please contact your HORIBA ABX representative service department 1 7 Transportation and Storage Conditions Storage temperature 20 C 68 F 50 C 122 F Storage humidity 85 or less Prior to the shipping of an instrument
29. by transporter whatever the destination an external decontamination of the instrument must be carried out 1 2 ABX Micros 200 Technical Manual RAA028A Specifications CONTENTS 1 Technical Specifications 2 2 2 Physical Specifications 2 2 3 ABX Micros CRP 200 description 2 4 4 ABX Micros CRP 200 description is 2 4 4 1 Front VIEW ier teet tro te mee end eee 2 4 4 2 Display M MA 2 5 4 3 Operation 0 2 6 4 4 Leftside ard Tear a i 2 7 4 5 Rightside VIEW HR 2 8 4 6 Sample tube holder tr iint a 2 9 4 7 Things to remember during 2 9 4 8 Precaution during operation or handling esses 2 10 4 9 Reagent door ene 2 11 ABX Micros 200 Technical Manual RAA028A 2 1 ABX Micros CRP 200 1 Technical Specifications Model ABX Micros CRP 200 Measurement mode CBC mode blood count item only CBC CRP mode blood count item CRP item QC CBC mode for CBC accuracy control QC CRP mode CRP Measurement item WBC RBC HGB HCT PLT CRP C reactive protein MCV MCH MCHC RDW MPV LYM LYM MON MON GRA GRA PCT PDW In the USA PCT and PDW parameters are for research use only and not for use in diagno
30. diluted bleach to 1 part of bleach for 4 of deionize water 1 5 Run F 76 function Prime cycle number ABX Micros 200 Technical Manual RAAO28A Micros 200 Enter 15 burn in cycles 1 in HGB display and 5 in display press Enter to exit function See Fig 1 Display page 3 ABX MiCPOS CRPeoo pies 3 n ES BUSY ERROR Fig 1 Display Run F 77 function Run burn in cycle and wait until the instrument stop 4 Drain and rinse 30 min Remove the 3 reagent straws from the bottle containing the diluted bleach Wrap the straws in absorbant paper Run two Prime cycles the bleach is drained Replace the diluted bleach by the distilled water bottle and re plunge the straws in distilled water Run six Prime cycles Rinse Remove the 3 reagent straws from the distilled water wrap the straws in absorbant paper Run two Prime cycles the distilled water is drained Run a Stand by cycle Re install the reagent bottles and the straws Switch off the instrument Close the instrument cover ABX Micros 200 Technical Manual RAAO28A RAS257 3 RAS257C RAS257 4 ABX Micros CRP 200 Technical Manual RAA028A Micros 200 RAS258C Yearly maintenance Concerns Yearly maintenance Required tools Hexagonal keys Torx keys Dynamometric screw driver A302 XEA586AS A301 XEA587AS A300 XEA585AS
31. displays Check the new adjustment install an empty reagent vial into CRP R2 Chamber and enter F 57 function press Enter Check that the needle is not touching the bottom of the vial If needle is not well located enter F 5b function and press Enter Needle is too much down decrease R2 needle value by the meaning of Up and Down keys 1 step for 0 1mm Needle is too much up increase R2 needle value by the meaning of Up and Down keys 1 step for 0 1mm Write down this value and press Enter to exit function This value is Needle R2 value in configuration ticket 4 ABX Micros 200 Technical Manual RAAO28A RAS261 7 RAS261C 7 Needle depth adjustment Enter F 81 function press Enter Close the tube holder Manually move down needle into the tube holder until contact with the bottom of one hole in any tube holder position Press Enter The status of the switches and the tube holder position are dispayed ABX Ini COS CRPaoo Switch 1 status Switch 2 status Switch 3 status em Tube holder position Press Enter again Needle moves up and needle motion value is displayed in WBC window ABX CRPaoo WBC 725 Needle motion value lt lt UB Switch 1 status Switch 2 status Switch 3 status Tube holder position Press Enter Sampling door is open and value is stored for the corresponding position If t
32. is not primed Connector is disconnected Cleaner is not primed Connector is disconnected Trouble displayed by code Actions Press online button Sequence restarts after data transmission finishes and error message is cleared after current measurement is completed Replace liquid temperature sensor Replace liquid temperature sensor Remove WBC and RBC black cover then check for bubbles in WBC chamber Clean the chamber put the cover back and run F62 function for blank measurement Run F96 function 40 minutes after turning on the power and check that 0 1 is blinking in WBC RBC windows Check the connector of the heater Check the exhaust fan operation Replace the cooler Run F96 function 40 minutes after turning on the power and check that 0 1 is blinking in WBC RBC windows Check the connector of the heater Check the exhaust fan operation Replace the cooler Check blank value variation by F91 If variation width is 10 counts or more air bubbles are present Clean the flowcell or replace it If checked values are ouside 3800 50 though no significant variations make adjustment Prime diluent Check the sensor connector Prime lyse Check the sensor connector Prime cleaner Check the sensor connector ABX Micros 200 Technical Manual RAA028A 7 5 ABX Micros CRP 200 code E31 E32 E33 E34 A35 A36 7 6 While air syringe is pressed Discharge error at 4 down and waste is
33. keys Barflex Required products None Intervention time 15min Frequency None Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS263C 1 Vacuum check Vacuum target from 220 mb to 190 mb Open the cover Switch on the instrument Disconnect second inlet from the top and connect the Barflex instead See Fig 1 Second inlet page 2 Fig 1 Second inlet Run F 69 function Pressure check Press ENTER the busy led is blinking and Vacuum syringe goes to generate vacuum Check that vacuum value read on the Barflex is within the following range from 220 mb to 190 mb Wait for 30s at least to check the stability of the vacuum The vacuum drop down must be less than 2 mb Press ENTER to exit function Disconnect the Barflex and connect back the tube on the Vacuum syringe If vacuum is not within the range check the O ring and the tube watertightness RAS263 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 RAS 640 Bubbling adjustment Concerns Bubbling adjustment Required tools Hexagonal keys Required products None Intervention time gt 10 mn Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operat
34. needle Backward Forward over the CRP mixing chamber Enter the auto adjustment of the CRP Value reagent depth The CRP cover opens Move the carriage to the CRP R2 position Locate the tip of the needle on the side of the drain hole Press the Enter key The pulse count is displayed Press the Enter key completed 1000 Units Enter the pulse count of the needle Up Down over the CRP mixing chamber Enter the auto adjustment of forward Value backward position of sample aspiration The Busy LED start blinking Locate sample holder s smallest hole in sampling position Close the sampling door Locate the needle in the center of the smallest hole and press the Enter key The pulse count is displayed Enter the pulse count of the needle Value forward backward position of sample aspiration ABX Micros CRP 200 Technical Manual 28 100 10 1 Units Units Units Carriage value 100 10 1 Units Units Units 100 10 1 Units Units Units 100 10 1 Units Units Units F6 Technician 2 F61 F62 F63 F64 F65 F66 F67 Needle sensor adjustment See 2 Needle home adjustment page 3 RAS261 HGB blank adjustment See HGB blank adjustment values table below See 8 HGB photometer adjustment page 8 RAS262 Aperture current adjustment See 6 Aperture voltage check page 6 RAS262 WBC latex adjustment See 9 WBC latex adjustment page 9 RAS262 PLT latex adj
35. one step for 0 1 mm If WBC display shows 000 re adjust the carriage home sensor position See Fig 2 carriage home sensor page 2 Fig 2 carriage home sensor his value is Sampling value on the configuration ticket E RAS261 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 2 Needle home adjustment Enter F 61 function press Enter Locate a plane part under the rinsing block See Fig 3 Needle home page 3 and manually move down the needle until contact with the plane part Fig 3 Needle home The correct position of the needle home detection sensor is when WBC display is empty and the value in RBC display is between 65 and 75 If the value is out of those limits a lt gt will be displayed in WBC window In this case the needle home detection sensor position must be adjusted See Fig 4 Needle home cell page 3 if the is in the upper part of the WBC window move the needle home sensor up If the is in the lower part of the WBC window move the needle home sensor down Fig 4 Needle home cell ABX Micros 200 Technical Manual RAAO28A RAS261 3 RAS261C 3 Needle heigth adjustment Remove chamber cover See Fig 5 Cover screws page 4 Fig 5 Cover screws Enter F 51 function press Enter The carriage moves over WBC chamber Manually move the needle down until it touch the edge of the WBC chamb
36. position F23 Carriage By pressing the Enter key the carriage is Left Right moved to the WBC chamber pos and the Busy LED starts blinking By pressing the Enter key again the carriage is moved forward to home position F24 Liquid By pressing the Enter key the liquid syringe syringe is moved up to the highest pos home and the Busy LED starts blinking By pressing the Enter key again the liquid syringe is moved down to the lowest position F25 Vacuum pressing the Enter key the Vacuum Waste Waste syringe is moved up to the highest syringe position home and the Busy LED starts blinking By pressing the Enter key again the Vacuum Waste syringe is moved down to the lowest position F26 Valves Check that the valves are opened closed Vaves N The valve N being checked is displayed on WBC display in order F27 Park The syringes and needle are moved to the system transfer position After running F27 turn the power OFF ABX Micros CRP 200 Technical Manual RAA028A 6 3 ABX Micros CRP 200 em F28 Door amp Check the status of the door and Tube Door Sensor 1 Sensor 2 Sensor 3 tube holder sensors 0 Close upper middle lower holder The status of sensors are displayed as 1 1 Open 0 Off 0 Off 0 Off sensor O 1 On 1 1 Press the Enter key to finish F28 The Ready LED lights up Control blood 0 1 0 Vacuum sample tube 0 0 1 Micro sample tube 0 1 1 Mini sample
37. potentiometer on mother board See Fig 1 Mother board page 2 2 3 PLT Threshold Adjustment Target 180 mV 5 Remove the MICROS CRP cover Switch on instrument Check between TP16 and TP31 Ground PLT threshold If necessary adjust PLT threshold by R82 potentiometer on mother board See Fig 1 Mother board 2 ABX Micros 200 Technical Manual RAAO28A RAS262 3 RAS262C RAS2 62 4 3 Motor voltages adjustment 3 1 Vacuum Syringe Target 2 5V 0 05 Remove the MICROS CRP cover Switch on instrument Check between TP36 and TP31 Ground Vacuum syringe motor voltage If necessary adjust Vacuum syringe motor voltage by R195 potentiometer on mother board See Fig 1 Mother board page 2 3 2 Reagent Syringe Target 2 5V 0 05 Remove the MICROS CRP cover Switch on instrument Check between TP35 and TP31 Ground Reagent syringe motor voltage If necessary adjust Reagent syringe motor voltage by R186 potentiometer on mother board See Fig 1 Mother board page 2 3 3 Carriage Target 1 5V 0 05 Remove the MICROS CRP cover Switch on instrument Check between TP37 and TP31 Ground Carriage motor voltage If necessary adjust Carriage motor voltage by R177 potentiometer on mother board See Fig 1 Mother board page 2 3 4 CRP Syringe Target 1V 0 05 Remove the MICROS CRP cover Switch on instru
38. return to home in the horizontal direction during the specified time frame CRP Liquid syringe does not return to home position in the specified time frame Sample needle does not return to home in the vertical direction during the specified time frame Printer does not accept print signal from the instrument Printer does not accept print signal printer paper is run out Printer does not accept print signal printer is turned off Content Vacuum syringe motor or position sensor is defective or has malfunctioned Liquid syringe motor or position sensor is defective or has malfunctioned 1 Sample needle carriage still has the shipment retaining clip secured 2 Sample carriage motor or position sensor is defective or has malfunctioned Liquid syringe motor or position sensor is defective or has malfunctioned 1 Sample needle is bent 2 Sample needle carriage motor or position sensor is defective or has malfunctioned 1 Reprint key was pressed during a function or a cycle 2 Printer is turned OFF No paper loaded when a result should be printed out or when the Reprint key is pressed Printer is not on line Actions Power OFF Wait 10 seconds Power ON and press the Startup Pause key to run Startup Power OFF Wait 10 seconds Power ON and press the Startup Pause key to run Startup 1 Power OFF remove instrument cover remove the black shipment clip from the lower
39. straws delivred with the instrument in the installation kit on the tubings the tubing with the label for the Miniclean straw Open the caps on the ABX Cleaner and ABX Lyse bottles Plunge the straws in the bottles then screw the caps RAS256 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 Set the ABX Cleaner on the right side of reagent door 1 Set the ABX Lyse to the left of the ABX Cleaner Be careful not to bend the tube when positioning the reagents 4 ln NNNM o PUN ABX LYSE N ABX CLEANER Let L ABX Micros 200 Technical Manual RAAO28A RAS256 5 RAS256C 3 3 Installing ABX CRP REA To enter the CRP reagent sensitivity factors please refer to RAS259 4 3 Input of the CRP reagent sensitivity factors page 6 install the ABX CRP REA only when measuring CRP gt This instrument is not provided with a function for checking the remaining quantity of CRP reagent The remaining quantity of ABX CRP REA should be checked visually When the remaining quantity decreases to below the lower portion of the CRP R3 reagent label replace all three reagents Take the ABX CRP REA CRP R1 CRP R2 and CRP R3 out of the refrigerator Push the lower front portion of the reagent door 2 on the main body side and open reagent door 2 4 Invert CRP R3 2 times Be sure to remove the cap and slowly set it in the r
40. the counting head before reassembling the electrode chamber counting head assy in order to avoid applying constraint on the chamber Position the chamber in its fixation clips then reconnect the tubes RAS258 6 ABX Micros 200 Technical Manual RAAO28A Micros 200 3 2 WBC chamber Counting head electrode seals replacement 1 x GBG275A 1 x FAA046A Proceed as described for RBC chamber to clean replace aperture seals and electrode O ring The spectrophotometer can not be dismantled from the chamber If this one has been dammaged it is necessary to replace the whole chamber assy When cleaning the spectrophotometer make sure to thorougly rinse it in order to obtain a correct HGB blank measure 4 CRP Syringe O ring replacement CRP syringe is located behind front panel to access this part you must remove the front panel See procedure RAS268 Front panel dismantling of this manual 1x FAA036A Switch off the instrument and disconnect power supply cable Remove the cover Remove the front panel Clamp and disconnect the CRP syringe tube See Fig 9 CRP syringe page 77 Unscrew the 2 CHC M2 5x25 fixation screws and remove CRP syringe 065021673 Fig 9 CRP syringe Open the syringe 3x Torx screws and remove O ring and piston Spread a little amount of silicone grease between two fingers and apply a very thin film of grease on the new O ring Install new O ring
41. the mean of Up and Down keys Press Enter Value blinking in RBC display shows the GRA target depending on the Latex lot used usually 183 2 if necessary you can change this value by the mean of Up and Down keys Press Enter the instrument is ready to sample WBC latex LYM or GRA Locate GRAN latex vial in tube holder and close sampling door cycle duration 3 min RBC display shows the actual measurement of the GRA latex volume When value is stable adjust GRA gain 183 2 by means of R74 potentiometer on mother board See Fig 1 Mother board page 2 At the end of the cycle average volumes and distribution curve are printed out Run F 64 function again using LYM latex ABX Micros 200 Technical Manual RAAO28A RAS262 9 RAS262C Check LYM value displayed in WBC window The value must be 52 1 4 If the LYM value displayed in WBC window is out of range 52 1 run F 64 function again using GRA latex and adjust GRA gain 183 2 by mean of R74 potentiometer on mother board Run F 64 function again using LYM latex and check LYM value displayed in WBC window For WBC gain the adjustment should only be done on GRA latex solution The LYM latex solution is used to check LYM gain only Never adjust WBC gain on LYM latex solution 10 RBC PLT latex adjustment As the RBC PLT gain is a factory adjustment it is mandatory not to readjust it without taking A the follow
42. 0 008 DBE018A DBE017A DBE021A XDA589CS F1000386000 E0013464600 G0166330 G0167040 H1008310001 H1008311001 H1008333002 H1008357001 H1008359001 H1008376001 G2002534 G2002535 H1001636001 H1008338002 DBE014A F1000391700 F1000391800 CHAMBER RBC BODY CRP200 CABLE COAX WBC MICROS 60 CHAMBER HB ASSY MICROS 60 CHAMBER WBC HB MICROS 60 CPTE CHAMBER APERTURE 80u O RING APERTURE D 0 5 P60 P80 CHAMBER COUNTING HEAD MIC 60 O RING COAXIAL CABLE SWITCH POWER ON OFF PCB POWER SUPPLY BOARD CRP200 CLIP FAN HOLDER ADHESIVE HOLDER FOR FLAT CABLE FAN POWER SUPPLY FAN CRP200 CABLE BUSHING D 9 5 BLACK FAN REAR FAN CRP200 COVER REAR FAN PROTECTION CRP2 CABLE BUSHING D 9 5 BLACK FAN MAIN FAN FILTER ASSY CRP200 FITTING LUER FEMALE I 3MM FITTING ANTI ROTATION WASHER REAGENT CONNEXION PLATE CRP200 SENSOR TEMPERATURE MIC LC220 PROTECTION SHIELD FOAM L 1M SYRINGE PLASTIC GUIDE L 63 5 CABLE BUSHING D 9 5 BLACK CABLE BUSHING D 23 8 BLACK CABLE BUSHING D 12 7 BLACK VALVE 7 LIQ VALVE ASSY 10 8 CUP OVERFLOW SYRINGES CRP200 PCB DISPLAY BOARD CRP200 MOTOR STEP BY STEP GEAR CRP200 SENSOR OPTIC CRP DIL SYR CRP200 SYRINGE PULLEY SYR LIQ AIR CRP200 SYRINGE WASHER SYR LIQ AIR CRP200 MOTOR CROSSPIECE S LIQ AIR CRP200 SYRINGE GEARING S LIQ AIR CRP200 SYRINGE COGGRAIL FOR LIQ SYR CRP200 MOTOR REDUCTOR PLATE ASSY CRP200 MOTOR REDUCTOR 3 SYR CRP200 MOTOR REDUCTOR CRP SYR CRP200 SYRINGE 3 SYR TRANS GUIDE CRP200 SYRINGE CRP T
43. 14510 18 49 21 3315YN 8 Y ONIMV3O 30I331X3 DRAWING NO VIRIOH V1010426 bl l al 0i 6 8 1 9 5 b 1 Analysis cycle technology CONTENTS 1 Measuring principles ia 5 2 le WBC and arde 5 2 1 25 RBC PLT sarono a 5 4 1 3 Hemoglobin measurement principle csse 5 5 1 4 Hematocrit measurement principle eee 5 5 1 5 RDW calculations icti da 5 6 1 6 MCHC calculation e 5 6 1 7 Measurihg the 5 6 1 8 Calculating PCT icc tire ndo ee Penta eese tre etus ema enda 5 6 1 9 Measuring the PD Wena ira a dci cia 5 6 1 10 CRP measuring principles 5 7 ABX Micros 200 Technical Manual RAA028A 5 1 ABX Micros CRP 200 1 Measuring principles 1 1 WBC and di ferential count 1 1 1 General counting principles The WBC measurement principles are the same as the RBC PLT measuring principles The WBC count is performed in the WBC HGB chamber The electronic signal processing device places an electronic threshold between the WBC and PLT signals The electronic pulses for the WBC are then placed into 256 channels according to their pulse size The pulses are then thresholded grouped and then mathematically calculated to create a numerical value for the determination of the WBC s 1 1 2 Differential Measuring principles The Diluent preserves and prepares the
44. 2 28 CRP unit view page 32 9 4 G0166610 G0166620 H1008358001 G2002319 7 H2003841001 G2002533 G2002318 H1008152001 GBF070A FAA017A GBC260AS G0166320 H1008317001 H1008355001 H1008373001 H2003334001 008 DBK014A DBK015A G0332140 L0163761 L0338230 F0022990300 CAE006A DAMOO6A G0166791 G0175390 G0289970 H1016977001 F1001306800 G0166801 H1031791001 DARO14A E1000072400 H1009391001 H1009392001 E0013099900 E1000349200 F0022990300 G0166461 G0166480 G0166511 Ne WwW LP O UO PW Qv Ui HRW UO PP Ut FP ON Ui UO TO PP H A UL PN Oo MOTOR STEPPER CRP200 SENSOR NEEDLE CARRIAGE CRP200 MOTOR PULLEY NEEDLE CRP200 KIT PULLEY ASSY CRP200 VALVE SOLENOID CRP BLOCK REAGENT SILICON STOPPER CRP200 CHAMBER CRP COLD ASSY CRP200 SENSOR DILUENT SENSOR CRP200 CABLE DILUENT SENSOR CRP200 CUP OVERFLOW AIR SYRINGE LC270 O RING TANK MIN AG WASTE MIC CHAMBER INJ WASTE VAC SYR BODY SENSOR VAC WASTE SYRINGE CRP200 CHAMBER WASTE VAC SYR PISTON SYRINGE COGGRAIL FOR AIR SYR SENSOR OPTIC DETECT TAB CRP200 CHAMBER WASTE CHICANE CRP200 SYRINGE PLASTIC GUIDE L 63 5 CABLE HOLDER RS FLAT CABLE CABLE HOLDER PRINT FLAT CABLE COVER FRONT COVER ASSY CRP200 COVER FRONT REAGENT DOOR CRP200 COVER KEYBOARD CRP200 COVER REAGENT DOOR LOCK CRP200 SWITCH MICROSWITCH XC5 81 82 VALVE SOLENOID MICROS CT PIERC SAMPLING BLOCK ASSY CRP200 SAMPLING STANDARD
45. 3 Inside screws RAS268 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 Disconnect all wirings from the front panel board 4 Disconnect tube holder s microswitch wirings Disconnect tube holder s openning system Remove front panel carefully 2 Front panel mounting Reassemble following steps of chapter 1 backward Following diagram shows connections for tube holder s microswitchs and openning system See Fig 4 front panel connections page 3 Refer to the labels on wirings for connection L1 E5 red L1 E6 yellow SW9 E10 blue SW9 E9 orange SW10 E12 blue pu 5W10 E11 purple SW11 E13 grey Fig 4 front panel connections Connect the 2 flat cables and the power supply cable on the Display board See Fig 5 Display board connections page 3 CRP board Keyboard flat cable flat cable Power supply cable Fig 5 Display board connections Switch on the instrument Check that all is operating normally ABX Micros 200 Technical Manual RAAO28A RAS268 3 RAS268C RAS268 4 ABX Micros CRP 200 Technical Manual RAA028A Micros 200 RAS 73C Autoconcentrated cleaning Concerns Instrument cleaning Required tools None Required products Minoclair Intervention time 15 mn Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab co
46. 4 233 238 243 25 232 237 242 26 231 236 241 27 230 235 240 28 229 234 239 29 228 233 238 30 228 233 238 31 227 232 237 32 226 231 236 33 225 230 235 34 224 229 234 35 223 228 233 6 8 ABX Micros CRP 200 Technical Manual 28 Software versions Software overview F7 Technician 3 Set up and display LRL CCC F71 Operating mode Factory use only 0 User Must be set to 0 1 Control E72 Serial number Instrument serial N is displayed 1000 Units 100 Units 10 Units 1 Units F73 Startup number N of Startup cycles is displayed 1000 Units 100 Units 10 Units 1 Units F74 Standby number N of Shutdown cycles is displayed 1000 Units 100 Units 10 Units 1 Units F75 Cycle number N of analysis cycles is displayed 1000 Units 100 Units 10 Units 1 Units F76 in cycle number of Burn in cycles is displayed 1000 Units 100 Units 10 Units 1 Units 577 Burn in Start Burn in cycle Burn in When stopping it turn OFF the cycle power number F78 LED Displays check Check the LED display Press the Enter key all LEDs light up at once Press again the Enter key the LED light up in order F79 Board type check 0 8p 255 16 18p F8 Technician 4 Tube holder F81 Automatic adjustment While the Busy LED blinks set a tube and close the Value See 7 Needle depth tube holder Manually move the needle to the bottom adjustment of the tube by the Reagent door 2 then press the page 8 RAS261 Enter key Press the Enter key ag
47. ABX Micros CRP 200 Technical manual P n RAA028A HORIBAASX 34184 MONTPELLIER Cedex 4 FRANCE Diagnostics Revisions revision RAA028A RAN153A V1 19 06 01 2006 This document applies to the latest software version as indicated above When a subsequent software version changes the information in this document a new section and or sections will be released Notice of liability The Information in this manual is distributed on an As Is basis without warranty While every precaution has been taken in the preparation of this manual Horiba ABX will not assume any liability to any persons or entities with respect to loss or damage caused or alleged to be caused directly or indirectly by not following the instructions contained in this manual or by using the computer software and hardware products described herein in a manner inconsistent with our product labeling Potential hazards To alert the operator of potentially hazardous conditions one of the bold captioned headings which are described below is provided wherever necessary throughout this text Flags a procedure that if not followed properly can prove to be extremely hazardous to either the operator or the environment or both Emphasizes an operating procedure that must be followed to avoid possible damage to the instrument or erroneous test results Emphasizes important information especially helpful to the operator before during
48. AS260 3 RAS260C Needle up amp cleaning carriage goes to sampling tube Valve 14 CRP syringe goes up Vacuum syringe goes up Valve 11 Valve 8 Liquid syringe goes up CRP reagent flap is closed Sampling needle carriage goes down Creation of a 4 ul air bubble in the needle while CRP chamber is drained Liquid syringe goes down Valve 18 Vacuum syringe goes up 8ul blood sampling for CRP count Valve 2 Vacuum syringe initialization Sampling needle carriage goes down Liquid syringe goes down Vacuum syringe goes down Movement over WBC chamber and needle exterior cleaning Vacuum syringe goes up Sampling needle carriage initialization Valve 11 Valve 8 Liquid syringe goes up Carriage initialization Carriage goes right Sampling needle carriage goes down Liquid syringe goes down Valve 5 Valve 2 Vacuum syringe initialization Valve 11 Liquid syringe goes up Needle up movement over CRP chamber rinsing block cleaning and CRP chamber drain Sampling needle carriage initialization Valve 8 Vacuum syringe goes up Carriage goes left CRP reagent flap is opened Valve 14 CRP syringe goes down Valve 18 Needle down into CRP chamber to distribute blood sample 8 and CRP R1 100 bubbling Valve 2 Vacuum syringe initialization Sampling needle carriage goes down Valve 15 CRP syringe goes up
49. CRP Position 4 910 5mm x 68mm for sampling cup 0 5 ml 3 7 Things to remember during operation 3 7 1 Sample When a whole blood sample is used use the anti coagulant EDTA K3 Also immediately after collecting the blood turn and mix the blood 10 times or more Before starting measurement slowly turn and mix 5 to 10 times Turbidity of the plasma etc will not affect the reaction However never allow foreign matters such as dirt bacteria and detergent to get into the mix 4 If the measurement range is exceeded dilute the specimen 3 7 2 Interfering substances Rheumatoid factor Rf bilirubin lipids abnormal proteins and hemoglobin in the sample do not affect the measurement of CRP only ABX Micros 200 Technical Manual RAA028A 2 9 ABX Micros CRP 200 3 8 Precaution during operation or handling When operating the instrument follow this manual 4 Observe Universal Precautions when operating this instrument or handling the specimen After the reagent is opened use it as soon as possible Close the lid of the container and store the reagent in accordance with the specified method Do not use any reagent after its expiration date has passed Do not use frozen reagent Latex particles will cause auto agglutination to generate sensitivity deterioration and mark error Mix ABX REA R3 before use Do not mix reagents of different lots Further in calibr
50. Check diluent level and prime EU on discharged to the tank Check air syringe for leakage p discharge pressure does not Check valve 5 and its tubings rise Check valve 5 and its tubings Pressure remains high and Check tube from air syringe to does not return to waste tank atmospheric level Check that waste tank is on atmospheric pressure level Discharge error at 4 pressure off While air syringe is pulled and waste is discharged from RBC and CRP chambers pressure does not Check air syringe for leakage Check valves12 13 and 18 are open Check diluent level and prime Discharge error at pressure on drop Check valves12 13 and 18 are Discharge error at 5 Pressure remains low and closed HE off does not return to Check tubings from chambers to p atmospheric level valves Temperature is out of the Use the instrument from 18 to Temperature is out of range 5 specified range 30 C CRP reagent blank is out of reagent blank is out of range range Set CRP REA properly ABX Micros 200 Technical Manual RAA028A Maintenance CONTENTS Te MA MENA CE 8 2 J RM pi coro 8 2 1 2 Daily customer maintenance uicit tee 8 2 1 3 Weekly customer 8 2 2 Maintenance Monarca 8 2 E AAA
51. Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS260C 1 Step by step analysis cycle introduction 1 1 Introduction The main cycle consists of six principal phases 1 CRP Preparation 1 2 Preparation Of CBC Dilutions And Counting 3 Preparation 2 4 During Preparation 2 Incubation 5 CRP Measurement 6 CRP After Cycle All phases are described in this procedure 1 2 Mechanical components Syringe and liquid syringe Vacuum syringe RAS260 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 Carriage and sampling needle carriage E Sampling needles carriage ce reagent flap o CRP Www rcagent 2 Step by step analysis cycle check 2 1 CRP Preparation 1 30s Carriage to CRP R1 Saponin and draining of needle rinsing block CRP reagent flap is opened Valve 8 Vacuum syringe goes up CRP syringe goes down Carriage goes right Creation of a 4pl air bubble in the needle while CRP chamber is drained Liquid syringe goes down Valve 18 Vacuum syringe goes up 1001 of CRP R1 reagent saponin sampling Valve 2 Vacuum syringe initialization Sampling needle carriage goes down Valve 15 CRP syringe goes down ABX Micros 200 Technical Manual RAAO28A R
52. E GUIDE CRP200 6 Sampling needle rinsing block view page 10 H1008308001 NEEDLE RINSING BLOCK CRP200 7 Carriage sensor amp pulley view page 11 G0167050 SENSOR OPTIC CARRIAGE CRP200 7 Carriage sensor amp pulley view page 11 G2002319 KIT PULLEY ASSY CRP200 8 LV2 view page 12 E1000349000 CABLE LIQ VALVE BLOCK 5 18 CRP 8 LV2 view page 12 XCA167A CHAMBER ISOLATOR SMALL 8 LV2 view page 12 XDA481B VALVE LIQ 2WAYS NC W O COIL 8 LV2 view page 12 XDA483B VALVE LIQ 3WAYS W O COIL 8 LV2 view page 12 F0023485400 CUP OVERFLOW RBC WBC CRP200 8 LV2 view page 12 588 5 VALVE 7 110 VALVE ASSY 18 5 9 RBC chamber view page 13 XBA413A CABLE COAX RBC MICROS 60 9 RBC chamber view page 13 FAA046A O RING COAXIAL CABLE 9 RBC chamber view page 13 XDA470ES CHAMBER RBC COMPLETE 9 RBC chamber view page 13 GBG275A O RING APERTURE D 0 5 P60 P80 9 RBC chamber view page 13 GBC239A CHAMBER COUNTING HEAD MIC 60 KRW FP O UL FP Pm tU W H 9 RBC chamber view page 13 H0525166003 CHAMBER APERTURE 500 9 2 ABX Micros CRP 200 Technical Manual RAA028A Exploded views Lu eee Ru uyu sssi drawing 9 RBC chamber view page 13 10 WBC chamber view page 14 10 WBC chamber view page 14 10 WBC chamber view page 14 10 WBC chamber view page 14 10 WBC chamber view page 14 10 WBC chamber view page 14 10 WBC chamber view page 14 11 Power supply view page 15 11 Power supply view page 15 11
53. E ONLY TO GRADE 1 DARO14A FUSE 3 15A 5X20 2 E1000072400 FILTER MAIN POWER CRP200 3 H1009391001 CABLE RS232 OUTPUT CRP200 4 H1009392001 CABLE PRINTER OUTPUT CRP200 9 30 ABX Micros CRP 200 Technical Manual RAA028A xploded views 27 Keyboard assy view 1 E0013099900 PCB KEYBOARD BOARD CRP200 2 E1000349200 CABLE DISPLAY KEYBOARD CRP200 3 F0022990300 COVER REAGENT DOOR LOCK CRP200 ABX Micros CRP 200 Technical Manual RAA028A 9 31 ABX Micros CRP 200 28 CRP unit view 1 G0166461 CHAMBER ADJ CRP UNIT ASSY UL 2 G0166480 SENSOR CRP DOOR SWITCH UL CRP200 B G0166511 CHAMBER CRP READ ASSY UL CRP200 4 G0332050 COVER PLASTIC REAG COVER 200 5 H1008320001 CHAMBER SAPONINE BOTTLE HOLDER 9 32 ABX Micros CRP 200 Technical Manual RAA028A Exploded views 29 Right side view 1 H1008348001 CARRIAGE BOTTOM HORIZ AXIS CRP 2 H1008350001 CARRIAGE TOP HORIZ AXIS CRP 3 H1008389001 COVER RBC WBC BLACK COVER ABX Micros CRP 200 Technical Manual RAA028A 9 33 ABX Micros CRP 200 30 Maintenance kit view 1 ON KR W 9 34 FAA017A F0020373000 FAA036A FAA046A FAA053A FAA055A H1008304002 GBG275A XEA931AS 8 O RING TANK MIN AG WASTE MIC O RING DILUENT PISTON CRP200 O RING FLOW CELL LYSE DISP MIC O RING COAXIAL CABLE O RING SAMPLING NEEDLE MICROS OT O RING MICROS SAMPLING SYRINGE SYRINGE DILUENT PISTON KELF O RING APERTURE D 0 5 P60 P80 KIT YEARL
54. GE value by the meaning of Up and Down keys 1 step for 0 1mm Repeat F 53 function until you get a correct adjustment ABX Micros 200 Technical Manual RAAO28A RAS261 5 RAS261C RAS261 6 5 Needle and carriage location over CRP chamber adjustment A Make sure that there is no CRP reagent vial in CRP assy Enter F 56 function and press Enter the carriage is moved over CRP glass chamber Manually move on the right hand side the carriage and move down the needle until it touch the edge on the right hand side of glass chamber See Fig 8 Needle on glass chamber page 6 Fig 8 Needle on glass chamber Press the Enter key to continue Needle moves up and carriage returns to home position Adjustment values are displayed WBC display shows CRP needle value RBC display shows CRP carriage value 4 Write down both value and validate to exit function Enter F 57 function press Enter Carriage is moved over CRP glass chamber and needle is coming down into it Check that needle is well centered into the CRP glass chamber Close sampling door and press Enter The needle is going inside CRP R2 chamber Press Enter The needle is going inside CRP R3 chamber Press Enter The needle is going inside CRP R1 chamber Press Enter to exit If needle is not well centered enter F 59 function and press Enter Needle is too much backward decrease CRP carriag
55. If you bypass the menu that you want to modify and or miss a step sequence when selecting a sub menu function you must do the following Re enter the Menu functions by pressing and holding the blue Service key Re select the sub menu within the menu that you have selected by using the Up or Down arrow keys Press the Enter key before releasing the blue Service key Carefully make your selection in the display windows by using the Up or Down arrow keys and then press the Enter key to accept the change and move to the next display window and or exit the menu F4 Laboratory Limits Select a number within a display window by using the Up or Down arrow keys and then press the Enter key to accept the change and move to the next display window and or exit the menu A Low Limits Set up the low limits WBC CBC MCV MCH MCHC F42 High limits Set up the high limits WBC RBC HGB HCT PLT CRP CRP CBC MCV MCH MCHC F43 PLT alarm Set up the threshold of PLT SCL SCH MIC adjustment flag F44 Low limits Set up the low limits of LYM MON GRA MPV RDW LMG LMG LYM MON GRA SEPT PDW F45 High limits Set up the high limits of LYM MON GRA MPV RDW LMG LMG LYM MON GRA PGT PDW F46 LMG alarms Set up the threshold of flags 11 M2 G1 G3 adjustment pug PCT and PDW are not used in the United States These parameters are strictly used for EZ investigational and research purposes only 1 2 Technician Menus Overview
56. LL LYSE DISP MIC SYRINGE 3 SYR BLOCK ASSY CRP200 O RING TEFLON 12uL SYRINGE CRP200 SYRINGE DIL BLOCK BODY CRP200 SYRINGE DILUTION BLOCK COVER SYRINGE DILUENT PISTON KELF SYRINGE LYSE PISTON CRP200 NEEDLE SAMPLE DISPENSER CRP200 ABX Micros 200 Technical Manual RAA028A Exploded views 5 CRP syringe view 1 2 3 4 5 G0166381 H1008322001 H1008323001 H1008305001 FAA036A SYRINGE CRP SYRINGE ASSY CRP200 SYRINGE CRP SYRINGE BODY CRP200 SYRINGE CRP BOTTOM PLATE CRP200 SYRINGE LYSE PISTON CRP200 O RING FLOW CELL LYSE DISP MIC ABX Micros 200 Technical Manual RAA028A 9 9 ABX Micros CRP 200 6 Sampling needle rinsing block view dl 2 3 4 5 FAA053A GBCO69AS DBK019A H1007659001 H1008308001 O RING SAMPLING NEEDDLE MICROS OT NEEDLE SAMPLING MICROS OT LC CLIP SAMPLING NEEDLE HOLDER NEEDLE GUIDE CRP200 NEEDLE RINSING BLOCK CRP200 ABX Micros 200 Technical Manual RAA028A Exploded views 7 Carriage sensor amp pulley view 1 G0167050 SENSOR OPTIC CARRIAGE CRP200 2 G2002319 KIT PULLEY ASSY CRP200 ABX Micros 200 Technical Manual RAA028A 9 11 ABX Micros CRP 200 8 LV2 view 6 1 1000349000 CABLE LIQ VALVE BLOCK 5 18 CRP 2 XCA167A CHAMBER ISOLATOR SMALL 3 XDA481B VALVE LIQ 2WAYS NC W O COIL 4 XDA483B VALVE LIQ 3WAYS W O COIL 5 F0023485400 CUP OVERFLOW RBC WBC CRP200 6 XDA588CS VALVE 7 LIQ VALVE ASSY 18 5
57. Manual RAAO28A 11 Summary adjustment table Adjustment WBC threshold WBC gain latex RBC threshold RBC gain PLT threshold PLT gain Vacuum syringe motor voltage Reagent syringe motor voltage Carriage motor voltage CRP syringe motor Needle motor voltage Pressure sensor Power supply check CRP blank HGB photometer Aperture voltage check Test point TPxx or function Fxx TP10 F64 TP13 F65 TP16 F65 TP36 TP35 TP37 TP38 TP16 on CRP board TP20 TP21 TP22 TP21 F91 F62 TP19 F63 TP31 TP31 TP31 TP31 TP31 TP31 TP31 Micros 200 R74 R75 R81 R82 R87 R195 R186 R177 R168 No adjustment required TP6 on CRP board TP31 TP31 TP31 TP31 TP31 VR2 on CRP board No adjustment No adjustment No adjustment No adjustment VR1 on CRP board R97 ABX Micros 200 Technical Manual RAAO28A R68 280 mV 5 LYM 52 1 GRA 183 2 400 mV 5 74 1 180 mV 5 64 1 2 5V 0 05 2 5V 0 05 1 5V 0 05 1 0V 0 05 1 7V 0 1 12 V 0 5 12 V 0 4 24 2 1 5 V 0 15 3800 10 See Tab RAS262 1 HGB channel page 8 60V 1 5 42 8 RAS262 11 RAS262C RAS262 12 ABX Micros 200 Technical Manual RAA028A Micros 200 RAS263C Vacuum check Concerns Vacuum check Required tools Hexagonal
58. Params CRP digits Select the number of decimal CRP DIGIT digits of CRP 0 1 Digit 1 2 Digits 6 10 ABX Micros CRP 200 Technical Manual 28 Alarm amp error list CONTENTS 1 Trouble not displayed by codes aria 7 2 2 Trouble displayed by code a 7 3 ABX Micros 200 Technical Manual RAA028A 7 1 ABX Micros CRP 200 Display code type Troubles that occur in the instrument include the following two types Trouble not displayed by code Trouble related mainly to the power supply Details are described forward in this Chapter Trouble displayed by code Trouble displayed by code on the sample display unit on the front of the main body Details are described forward in this Chapter 1 Trouble not displayed by code Before concluding that the instrument has broken down check the following points For whom of contact see the back cover Instrument does not operate even if power is turned ON Is the power cable connected Turn OFF the power switch then connect the power cable Is reagent door 2 open Close reagent door 2 Is the fuse blown Check the fuse holder at the back of the instrument referring to Section 07 Maintenance Replace the blown fuse Key is not accepted Is the instrument in the sleep state Press the Start up Pause key to light up the Ready LED Is reagent door 2 open Close reagent door 2 Is the error cod
59. RANS GUIDE CRP200 CABLE BUSHING D 3 2 BLACK BELT NEEDLE 285MM CRP200 BELT CARRIAGE 650MM CRP200 ABX Micros CRP 200 Technical Manual RAA028A 9 3 ABX Micros CRP 200 Lures ads drawing 17 Carriage view page 21 17 Carriage view page 21 17 Carriage view page 21 17 Carriage view page 21 18 CRP cold assy view page 22 18 CRP cold assy view page 22 18 CRP cold assy view page 22 19 Reagent sensor 1 view page 23 19 Reagent sensor 1 view page 23 20 Air syringe cup view page 24 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 21 Air syringe view page 25 22 Main board view page 26 22 Main board view page 26 22 Main board view page 26 22 Main board view page 26 23 Front panel view page 27 23 Front panel view page 27 23 Front panel view page 27 23 Front panel view page 27 24 Sample holder view page 28 24 Sample holder view page 28 24 Sample holder view page 28 24 Sample holder view page 28 24 Sample holder view page 28 24 Sample holder view page 28 25 Cover view page 29 25 Cover view page 29 25 Cover view page 29 26 Back view page 30 26 Back view page 30 26 Back view page 30 26 Back view page 30 27 Keyboard assy view page 31 27 Keyboard assy view page 31 27 Keyboard assy view page 31 28 CRP unit view page 32 28 CRP unit view page 3
60. T CNZ CN6 e Display board blank adjustment CRP NS 5 a measurement unit VR1 Pressure sensor e Reagent yal cooling unit TP16 mu Pressure sensor VR2 adjustment Mother board ____ Liquid valves ah 18 12 7 4 9 9 13 5 TP6 CNTO CN5 CN12 CNT CNM CN9 Security Power Diluent Cleaner Lyse Mixing chamber Magnet Supply Sensor Sensor Sensor ABX Micros 200 Technical Manual RAAO28A Fig 3 CRP board 5262 7 RAS262C 8 HGB photometer adjustment A The HGB photometer calibration must be done 20 min at least after the instrument has been switched on This adjustment must be done with the WBC chamber cover installed If the WBC chamber has been dismantled previously make sure no liquid has flown in between the spectrophotomer and the chamber Clean the inner surfaces of the spectrophotometer as well as the chamber Reassemble the assy and tighten the two screws to the following torque 400mN m Remove the MICROS CRP cover Dismantle the WBC HGB chamber cover Check the general cleanliness of the WBC chamber spectrophotometer assy Re install the chamber cover Switch on instrument Run F 62 function HGB photometer adjust after 40 seconds approximately the function is automatically exited The curent channel is displayed on HGB display See Fig 4 HGB display page 8 ABX Imi COS CRPaoo Fig 4 HGB display By means of R97
61. TUBE HOLDER SAMPLING BRAKING GEAR SPRING COVER SAMPLING BLOCK DOOR CRP200 COVER SIDE DOOR LOCK ASSY CRP2 COVER MAIN COVER ASSY CRP200 COVER CRP REAGENT DOOR CRP200 FUSE 3 15A 5X20 FILTER MAIN POWER CRP200 CABLE RS232 OUTPUT CRP200 CABLE PRINTER OUTPUT CRP200 PCB KEYBOARD BOARD CRP200 CABLE DISPLAY KEYBOARD CRP200 COVER REAGENT DOOR LOCK CRP200 CHAMBER ADJ CRP UNIT ASSY UL SENSOR CRP DOOR SWITCH UL CRP200 CHAMBER CRP READ ASSY UL CRP200 ABX Micros CRP 200 Technical Manual RAA028A xploded views E AA s i drawing 28 CRP unit view page 32 28 CRP unit view page 32 29 Right side view page 33 29 Right side view page 33 29 Right side view page 33 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 30 Maintenance kit view page 34 31 Printer view page 35 31 Printer view page 35 31 Printer view page 35 32 Straw and tubing view page 36 32 Straw and tubing view page 36 32 Straw and tubing view page 36 G0332050 H1008320001 H1008348001 H1008350001 H1008389001 FAA017A F0020373000 FAA036A FAA046A FAA053A FAA055A H1008304002 GBG275A XEA931AS CBE053A CBE057A 058 XDA693A GBC284A GBC285A Ut WON KRW WY ABX Micros CRP 200 Technical M
62. Vol ABX CRP REA R3 Latex 200 ul Final dilution rate 2 100 Incubation time 20 sec Measurement Method Photometry Wavelength 850nm Measurement duration 60 sec ABX Micros 200 Technical Manual RAA028A 5 7 ABX Micros CRP 200 1 10 2 CRP Reagent principles ABX CRP REA R1 Hemolysis reagent on whole blood lyses RBC After lyse of RBC antigens are still present in the solution ABX CRP REA R2 buffer reagent inhibits all different antigens except C Reactive protein Only C Reactive proteins are present in the solution ABX CRP REA R3 latex reagent agglutinates C Reactive proteins around Latex balls Agglutinations of C Reactive proteins and Latex are ready to be measured 5 8 ABX Micros CRP 200 Technical Manual RAA028A Analysis cycle technology Measuring principles 1 10 3 CRP Measurement principle Absorbance measurement C Reactive proteins and Latex agglutinations are measured by spectrophotometry through the optical part of the CRP chamber with a wavelength of 850nm First measurement is performed after 20 sec Then 1 measure each 10s for a total of 7 measurements The first absorbance measurement at 20 sec is used to trigger two different flags on display E on printer means that Diluent has ran out dilution is too low EE E on display P on printer means that CRP value is in Prozone In any case the cycle will be ended 1 10 4 CRP Calculation ABX Mi
63. WBC cell membrane for differentiation reaction The Lyse has specific reactions with each sub population of the WBC cytoplasmic membranes When the Lyse reacts with the Lymphocytes cytoplasmic membranes it allows the release of water soluble cytoplasm and shrinks the cell membrane around the nucleus When the Lyse reacts with the Monocytes cytoplasmic membranes it has an intermediate reaction maintaining it s large size in comparison to the Lymphocytes When the Lyse reacts with the Granulocytes cytoplasmic membranes it has a limited reaction due to a molecule in their cytoplasmic structure which protects them from the shrinking action of the lyse This limited reaction makes the Granulocytes the largest of the sub populations in the cell differentiation After the differential lysing action the ABX Micros CRP 200 analyzes the height of each pulse as the cells pass through the micro aperture in the WBC chamber These pulses are then channelized thresholded grouped according to their size 30fL to gt 450fL and calculated mathematically to create the WBC distribution curve which is also known as the WBC Histogram The 3 sub populations of WBC s are placed according to the number of cells and the size of cells in each sub population The distribution of WBC s are as followed Lymphocytes 30fL to 100fL Monocytes 100fL to 150fL Granulocytes 150 1 to 450fL This differentiation term is also known as LMG s Lymphocytes
64. Y MAINTENANCE CRP200 ABX Micros 200 Technical Manual RAA028A Exploded views 31 Printer view mem DPU 414 printer OFF LINE PAPER END um ml ON LINE FEED Number Reference Designation 1 CBE053A PRINTER SEIKO DPU414 W O CORD 2 CBE057A PRINTER DPUA414 SUPPLY 220V 6V 3 CBE058A PRINTER DPUA414 SUPPLY 110V 6V ABX Micros 200 Technical Manual RAA028A 9 35 ABX Micros CRP 200 32 Straw and tubing view 1 2 3 4 9 36 XDA693A GBC284A GBC285A G0166911 TUBING WASTE P60 CRP200 REAGENT STRAW CLEANER CRP200 REAGENT STRAW WASTE CRP200 REAGENT STRAW DILUENT CRP200 Not available at the time of publication ABX Micros CRP 200 Technical Manual RAA028A xploded views 33 Waste container view Number Reference Designation 1 G0166861 REAGENT WASTE CONTAINER CRP2 ABX Micros 200 Technical Manual RAA028A 9 37 ABX Micros 200 9 38 ABX Micros CRP 200 Technical Manual RAA028A
65. ain to validate the setup F82 Position check Position check of needle Up Down over the sample See 7 Needle depth holder adjustment page 8 RAS261 F83 Not used F84 Change position 2 value Enter the pulse count of needle Up Down over a 1000 100 10 Units See 7 Needle depth control vial adjustment page 8 RAS261 F85 F86 Change position 4 value Enter the pulse count of needle Up Down over 1000 100 10 Units vacuum sample tube F87 Change position 5 value Enter the pulse count of needle Up Down mini 1000 100 10 Units sample tube F88 Change position 6 value Enter the pulse count of needle Up Down over 1000 100 10 Units micro sample tube ABX Micros CRP 200 Technical Manual RAAQ28A 6 9 ABX Micros CRP 200 F9 Technician 5 CRP F91 CRP photometer adjust Press the Enter key then adjust Value See 7 CRP blank VR1 on CRP board to have adjustment 3800 50 on WBC display page 7 RAS262 F92 CRP 1 value adjust Do not change 0 2 6 8 4 F93 CRP 2 value adjust Do not change 0 0 8 0 2 F94 CRP 3 value adjust Do not change 0 2 5 9 5 F95 CRP 4 value adjust Do not change 0 4 0 0 0 F96 Cooler heater check Check the motor of switch ON Heater Cooler See 2 Diluent OFF of cooler and heater 0 Off 0 Off temperature in CRP 1 On 1 On chamber check page 3 RAS265 F97 Default values Set the instrument parameters to default values F98 System Config Select the number of Param measurement items 0 16 Params 1 18
66. al Manual RAAO28A RAS258 9 RAS258C Fig 14 Bottom plate Pull out the pistons 1 2 and 3 from the body with their respective O ring still around See Fig 15 Pistons page 10 Fig 15 Pistons Replace all O rings by new ones Replace Diluent piston H1008304002 and check other pistons and syringe body cleanliness If necessary clean with a soft paper Spread a little amount of silicone grease between two fingers and apply a very thin film of grease on each new O ring Reinstall the liquid syringe in reverse order Use the A302 dynamometric screw driver to tighten the 4 CHC screws A to 700mN m Use the A301 dynamometric screw driver to tighten the 2 FHC screws B to 400mN m RAS258 10 ABX Micros CRP 200 Technical Manual RAAO28A Micros 200 6 End of maintenance 6 1 Cleaning Remove and clean the CRP protection then put it back See Fig 16 CRP protection page 11 Fig 16 CRP protection 6 2 Lubricating Clean the carriage and the needle carriage axes See Fig 17 axes page 11 Put one drop of oil on each axes Fig 17 axes Disconnect the diluent and waste inputs located at the rear of the instrument Move the vacuum syringe by hand in order to have an access to the motor gearings Spread a little amount of grease on the gearings and on coggs of the piston axis See Fig 18 Vaccum syringe lubricating page 12 ABX Micros CRP 200 Te
67. al view 1 1 Test points Adjustment Test point Potentiometer Target value WBC line adjustment Factory adjusted WBC threshold TP10 TP31 R68 280 mV 5 RBC line adjustment TP14 Factory adjusted RBC threshold TP13 TP31 R75 400 mV 5 PLT line adjustment TP18 Factory adjusted PLT threshold TP16 TP31 R82 180 mV 5 Power supply check TP20 TP31 No adjustment 12 V 0 5 TP21 TP31 No adjustment 12 V 0 4 TP21 TP31 No adjustment 5 V 0 15 TP22 TP31 No adjustment 24 V 2 1 Reagent syringe motor voltage TP35 TP31 R186 2 5V 0 05 Vacuum syringe motor voltage TP36 TP31 R195 2 5V 0 05 Carriage motor voltage TP37 TP31 R177 1 5V 0 05 CRP syringe motor TP38 TP31 R168 1 0V 0 05 Aperture voltage check TP19 TP31 No adjustment 60V 1 5 2 8 4 2 ABX Micros 200 Technical Manual RAA028A Electric amp electronic principles Main board general view O HGB J38 J39 ETP31ETP30 29 assembly J21 Temperature 2 g p be o sensor gt RBC Coax WBC Coax HGB TP32 2 gt s EE 5 BE 1 1 1 MER 27 E 14 25 R16 ES ag m R80 _ a 19 Threshold Threshold Aperture Threshold m Voltage J TP4 1 1 Fon ca m c Bus 2 11 PLT Pulse Pro
68. aning Sampling needle initialization Valve 2 Liquid syringe initialization Valve 8 Vacuum syringe goes up Valve 2 Vacuum syringe initialization Liquid syringe goes down First dilution sampling for second dilution Sampling needle carriage goes down Liquid syringe goes down 28 5yl sampling of first dilution in WBC chamber Sampling needle carriage goes up Air drawing inside needle needle cleaning and initialization transfer from WBC chamber to RBC chamber Liquid syringe goes down Liquid syringe goes up Vacuum syringe goes up Valve 11 Liquid syringe goes up Sampling needle carriage initialization Valve 8 Valve 2 Carriage right over RBC chamber RAS260 6 ABX Micros 200 Technical Manual RAAO28A Micros 200 Second dilution in RBC chamber and lyse of first dilution in WBC chamber Sampling needle carriage goes down in RBC chamber Vacuum syringe goes down Valve 12 Control of the temperature Valve 2 Vacuum syringe goes down Valve 1 Liquid syringe goes up inject 0 5ml of lyse in WBC chamber Valve 11 Liquid syringe goes up 0 5ml of diluent outside needle Vacuum syringe goes down Valve 10 Bubbling of dilution in WBC chamber Valve 12 Vacuum syringe goes down Bubbling of dilution in RBC chamber Valve 13 Vacuum syringe goes down Liquid syringe goes down Sampling needle initializatio
69. anual RAA028A COVER PLASTIC REAG COVER CRP200 CHAMBER SAPONINE BOTTLE HOLDER CARRIAGE BOTTOM HORIZ AXIS CRP CARRIAGE TOP HORIZ AXIS CRP COVER RBC WBC BLACK COVER O RING TANK MIN AG WASTE MIC O RING DILUENT PISTON CRP200 O RING FLOW CELL LYSE DISP MIC O RING COAXIAL CABLE O RING SAMPLING NEEDLE MICROS OT O RING MICROS SAMPLING SYRINGE SYRINGE DILUENT PISTON KELF O RING APERTURE D 0 5 P60 P80 KIT YEARLY MAINTENANCE CRP2001 PRINTER SEIKO DPU414 W O CORD PRINTER DPU414 SUPPLY 220V 6V PRINTER DPU414 SUPPLY 110V 6V TUBING WASTE P60 CRP200 REAGENT STRAW CLEANER CRP200 REAGENT STRAW WASTE CRP200 9 5 ABX Micros CRP 200 2 CRP board view 1 2 3 4 5 E0012926700 G1001410 E1000349100 E1000334900 E1000348900 PCB BATTERY ASSY CRP200 PCB CRP BOARD CRP200 CABLE CRP DISPLAY 200 ADHESIVE FERRITE CRP200 CABLE LIQ VALVE BLOCK 8 10 CRP ABX Micros 200 Technical Manual RAA028A xploded views 3 Reagent sensor 2 view 1 G2002320 SENSOR REAG SENSOR 2 WAY CRP 2 H1008152002 CABLE CLEANER SENSOR CRP200 3 H1008152003 CABLE LYSE SENSOR CRP200 ABX Micros CRP 200 Technical Manual RAA028A 9 7 ABX Micros CRP 200 4 Liquid syringe view 1 ON KR W ps e F0020373000 FAA055A FAA036A G0166380 H1008301001 H1008302001 H1008303001 H1008304002 H1008305001 H1008331001 O RING DILUENT PISTON CRP200 O RING MICROS SAMPLING SYRINGE O RING FLOW CE
70. at must be A worn by the operator Local or national regulations must be applied in all the operations RAS273C 1 Concentrated cleaning 1 1 Drain all chamber Switch on the instrument Run a Startup cycle Run F 12 function Drain chamber 1 2 CBC Cleaning Open the cover and remove the chamber cover Fill RBC and WBC chamber with minoclair 5ml for each chamber Switch on the instrument Run F 16 function CBC bleach 1 3 CRP Cleaning Fill CRP chamber with minoclair 5ml Run F 17 function CRP bleach RAS273 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 RAS445 A CRP unit replacement Concerns CRP unit replacement Required tools Hexagonal keys Required products None ntervention time 15 mn Frequency On request Specific kit or consumables CRP unit Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS445A 1 CRP unit dismantling Turn off the instrument then disconnect the power supply cable Open the cover Unscrew the 2 CHC screws then remove the chamber cover See Fig 1 cover screws page 2 Fig 1 cover screws Remove the WBC and RBC chamber from the clamps See Fig 2 Chambers page 2 Fig 2 Chambers Disconnect the tubing from the T connector T4 1 to the flowcell See Fig 3 tubing page 2 Fig 3 tu
71. ation and sample measurement be sure to use reagent of the same lot Avoid mixing leftover reagent Use the same lot of ABX REA R1 ABX CRP REA R2 and ABX CRP REA R3 Take care so that dirt does not get into the reagent and cell ABX CRP REA R1 ABX CRP REA R2 and ABX CRP REA R3 contain sodium azide of 0 1 or less Sodium azide may generate explosive metal azide when reacted with zinc or copper pipes When disposing of this solution use a large quantity of water For treatment of waste liquid refer to Chapter 5 Daily maintenance Treatment of waste liquid All suspect results should be rechecked by reanalysis and or alternative method Results from the instrument should be used in combination with the clinical symptoms of the patient and the test results 2 10 ABX Micros 200 Technical Manual RAA028A Specifications ABX Micros CRP 200 description 3 9 Reagent door inside ABX Lyse ABX Miniclean Reagent setting position Reagent ABX lyse and ABX Miniclean are set as illustrated ABX Micros 200 Technical Manual RAA028A 2 11 ABX Micros CRP 200 2 12 ABX Micros 200 Technical Manual RAA028A Hydraulic amp Pneumatic principles CONTENTS II rU mr 3 2 1 1 Hydropneumatic connections errar 3 2 1 45 Functions iM aad 3 4 2 Pneumatic diagram
72. be modified only when hematologic erroneous results are given by the instument If bubbling is too important liquid overflows can occur or if bubbling is too low homogeneity of the dilution can be decreased Increase or decrease Bubbling 2 value blinking in RBC display with Up and Down keys Press Enter to exit function ABX Micros 200 Technical Manual RAAO28A RAS264 3 RAS264C RAS264 4 ABX Micros 200 Technical Manual RAA028A Micros 200 RAS265C Thermic adjustment Concerns Thermic adjustment Required tools Hexagonal keys Thermometer Required products None Intervention time 2 30 Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS265C 1 Diluent temperature sensor adjustment Plunge thermometer probe deeply into diluent container Run 2 Diluent prime F 13 functions Enter F 67 function Temperature calibration See Fig 1 F67 display page 2 ABX MiCPOS CRPaoo CYCLE HGB wor vo gt BUSY ERROR Fig 1 F67 display Temperature is displayed WBC display With Up and Down keys input temperature read on thermometer and press Enter Temperature must be input as quickly as possible after priming If not diluent temperature will A raise in s
73. bing Cut the tyraps then disconnect CN9 from the CRP board Disconnect the connectors on the 2 boards of the CRP unit then unscrew the CHC screws See Fig 4 CRP board connectors page 3 RAS445 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 Fig 4 CRP board connectors Remove the CRP unit See Fig 5 CRP unit page 3 Fig 5 CRP unit Unscrew the CHC screws then remove the LED board and the PD board See Fig 6 boards page 3 Fig 6 boards 2 CRP unit replacement Install those boards on the new CRP unit Connect the connectors on the 2 boards Install the new CRP unit with the two CHC screws do not tight the screws Pass the connector through the hole of the frame then connect it on the CRP board CN9 Connect the tubing on the T connector then fix it in the clamp Install the WBC and RBC chambers back into their respective clamp ABX Micros 200 Technical Manual RAAO28A RAS445 3 RAS445A 3 Adjustments Manually move the carriage over the CRP chamber then move the needle down Adjust the CRP unit position to have the needle centered in the CRP chamber See Fig 7 needle centering page 4 then tighten the screws Fig 7 needle centering Manually move the needle up Connect the power supply cable then turn on the instrument Enter F 56 function press Enter Carriage is moved over CRP glass chamber Ma
74. cal Manual RAA028A Micros 200 3 CBC Calibration Calibration has to be done always on a clean and reproducible instrument blank values must be A in the acceptable limits The calibration can be achieved on one parameter or more at the same time Turn on the printer and switch on the instrument Press Mode key to select CBC mode Prepare the calibrator according to the specific instructions temperature mixing etc Measure the calibration blood six times After eliminating the maximum and minimum values acquire the mean value of the remaining four data for each of the six measurement item results WBC RBC HGB HCT PLT MPV Calculate the ratio Assay value Mean value of four data for each parameter Press Calibration key on the operation panel then press Reprint key Currently stored calibration coefficients are printed out COEFFICIENTS DATE 04 01 2005 TIME 16 37 WBC B Li RBC 0 8 HGB 1 2 HCT B 1 03 Calibration PLT 1 1 coefficients MPV 0 9 RDW 0 8 CRP 1 0 0 90 CRP FCT B 1 04 CRP FCT C z 0 87 CRP BLANK 14 12 2004 11 30 Multiply each printed calibration coefficient by each ratio previously calculated to obtain the new calibration coefficient New calibration coefficient displayed calibration coefficient x ratio New calibration coefficient for each item should be in the following range Calibration range Parameter
75. ce key In order to work on the instrument and to run cycles without the cover place a magnet over proximity sensor located on the reagent cooling unit Place a magnet on the proximity sensor 8 2 ABX Micros CRP 200 Technical Manual RAA028A 2 Maintenance kit Maintenance Maintenance kit Follow the RAS258 Yearly maintenance procedure for maintenance with XEA931AS kit The kit includes FAA017A O RING TANK MIN AG WASTE MIC 1 F0020373000 15x1 5 Nit 70SH O ring 1 FAA036A O RING FLOW CELL LYSE DISP MIC 2 FAA046A O RING COAXIAL CABLE 2 FAA053A O RING SAMPL NEEDLE MICROS OT 1 FAA055A O RING MICROS SAMPLING SYRINGE 2 H1008304002 Liquid Syringe Diluent Piston 1 GBG275A O RING APERTURE D 0 5 P60 P80 4 3 Procedures A E Maintenance and adjustments that need to be done on Micros CRP 200 are divided up into procedures according to the specific assemblies This should make any update easier as all interventions can be carried out with the corresponding procedure on its own 4 Each procedure has to be read in full before beginning the intervention P N and tittle RAS256 RAS257 RAS258 RAS259 RAS260 RAS261 RAS262 RAS263 RAS264 RAS265 RAS268 RAS273 RAS445 Installation Decontamination amp rinse Yearly maintenance Check up after intervention Step by step analysis cycle Needle amp carriage adjustment Main board amp CRP board Vacuum check Bubbling adjustme
76. cess RBC Pulse Process WBC Pulse Process RS232 J22 TP2 TP1 Supplies DS6 20 213 rTP6 DS3 22 111 DS4 amp TP23 x DS5 TP21 M s10 CRP Printer J5 board Liquid valves 8 2 14 15 co 45v a 19 a O 9 amp co Power HE Ez Nx Ground supply 136 12 Ni 1 the J26 Vacuum Syringe Reagent Syringe Carriage CRP Syringe 24V 12V 12V Cycle start gt id Fan Piercer 27 Motor Sensor Motor Sensor Motor Sensor VACUUM SYRINGE REAGENT SYRINGE CARRIAGE CRP SYRINGE ABX Micros CRP 200 Technical Manual RAA028A 4 3 FOLE PITAM AELE TLC 7 AS eun PICOS YALSUN SOBIIM WSITIdINOM BIPE susnpus AP ena BET 3 XOU ABX Micros CRP 200 Technical Manual RAA028A CONTRA FING Q31 42 52 52 57 2 2 22 CUOTIEUNETJUOZ 04257 08257 gt u 22 97 19 ses Z T 3 Z T fes United States no display with Hemalink English Japan 3 nejap 32auuo g ZZf apo ueg 1 dOj SOUTH 8 22 H NO I SNA J ysa 91 P i2 uuo2 you 510 514 conter gt USG Oi P3328UU02 515 514 1 FIG pg zZZr Sezer 2 2 canes 1 Qui 8 22 Oy Ecer 1 f T Sa E cl 3581 amp hiTS UI PRIDAULOS
77. chnical Manual RAAO28A RAS258 11 RAS Fig 18 Vaccum syringe lubricating Manually move the syringe assembly to spread the grease on all parts of the gearings and piston axis Fig 19 Manual moving Spread a little amount of grease on the coggs of the piston axis of CRP syringe and liquid syringe Fig 20 CRP amp reagent syringes lubricating RAS258 12 ABX Micros 200 Technical Manual Micros 200 Finish the front panel mounting 7 Check Check for reagents and diluent quantities remaining Check that cables flat cables connectors and tubings are all connected Connect power supply cable Switch on printer and instrument Run 2 Prime cycles Close sampling door to start a blank cycle During this cycle check for Watertightness of Waste CRP and Liquid syringe Watertightness of rinsing block Unusual mechanic noises Chambers draining Good opening of CRP block flap Then proceed to the Checkup after intervention Procedure RAS 259 It is mandatory to proceed to Checkup after intervention procedure after maintenance This A procedure will make sure you get the best instrument reliability See procedure RAS 259 in this manual ABX Micros 200 Technical Manual RAAO28A RAS258 13 RAS258C RAS258 14 ABX Micros 200 Technical Manual RAA028A Micros 200 RAS259C Check up after intervention Concerns Checkup after intervent
78. cros CRP 200 calculate absorbance for each measure ABSORBANCE MEASUREMENT E a 30 40 50 60 70 3 Time sec 0 0 23 e C gt Each absorbance is compared to a CRP Blank Value wich measurement is from diluent Blank measurement Absorbance Log Measured Value 1 10 5 CRP calibration curve Absorbance slope are calculated between two following absorbance measures Absorbance slope average will give C Reactive protein concentration by projection onto CRP Calibration curve ABX Micros CRP 200 Technical Manual RAA028A 5 9 ABX Micros 200 Absorbance slope 0 18 0 16 0 14 0 12 0 10 0 08 0 06 0 04 0 02 0 20 40 60 80 100 CRP concentration 1 10 6 CRP flagging areas Dil After the first 20 seconds of the reaction if the CRP concentration is over 20 mg dL the CRP display window will display EE E and D on the printed report The flagging diagram is used to illustrate the reaction curve of the CRP concentration when it is above the upper limit of the assay 20 mg dL Note the difference in the reaction curves between serum and whole blood Empty When a known CRP concentration value is under 15mg dL and the absorbance at the first 20 seconds of the reaction is over the HCT x Coefficient the CRP display window will display and E on the printed report 5 10 ABX Micros CRP 200 Technical Manual RAA028A Analysis cycle techno
79. d QC CBC data are deleted F07 Delete saved QC CRP data Saved QC CRP data are deleted 6 2 ABX Micros CRP 200 Technical Manual 28 Software versions Software overview F1 Miscellaneous Cycles Displayed in the N Window F11 Backflush Applies back pressure on the counting apertures F12 Drain chamber Drains all counting chambers F13 Prime Diluent Primes the ABX Minidil LMG into the system F14 Prime lyse Primes the ABX Lyse into the system F15 Prime Cleaner Primes the ABX Cleaner into the system F16 Bleach Clean CBC Used to remove protein buildup and fibrin clots F17 CRP prime Primes the ABX Minidil LMG into the CRP flowcell F18 Complete Rinse cycle Rinses the fluidic system with ABX Minidil LMG F2 Mechanical Cycles Select a number within a display window by using the Up or Down arrow keys and then press the Enter key to accept the change and move to the next display window and or exit the menu RCE m F21 Sensors The status of sensors are displayed as 1 or Sample Sample Liquid Vacuum 0 needle Carriage syringe Waste m Manually move the parts to check that the 0 Down 0 Back 0 Down syringe 0 Down sensors are turned ON OFF properly 1 Up 1 Forward 1 2 Up 1 Down 1 Up 0 Up F22 Sample By pressing the Enter key the needle is needle moved down to the lowest position and the Up Down Busy LED starts blinking By pressing the Enter key again the needle is moved up to home
80. e value by the meaning of Up and Down keys 1 step for 0 1mm or Needle is too much forward increase CRP carriage value by the meaning of Up and Down keys 1 step for 0 1mm ABX Micros 200 Technical Manual RAAO28A Micros 200 If needle is too low or too high enter F 58 function and press Enter Needle is too much down decrease CRP needle value by the meaning of Up and Down keys 1 step for 0 1mm Needle is too much up increase CRP needle value by the meaning of Up and Down keys 1 step for 0 1mm Write down this value and press Enter to exit function Restart F 57 function and repeat until you get a correct adjustment The needle must not touch the bottom of the CRP chamber Those values are CRP Needle and ET CRP Carriage values on configuration ticket 6 Needle and carriage location over CRP R2 reagent chamber adjustment A Make sure that there is no CRP reagent vial in CRP assy Remove the CRP plastic cover Enter F 5a function press Enter Carriage is moved over CRP R2 reagent chamber Manually move down needle into chamber See Fig 9 CRP R2 needle location page 7 CRP R2 reagent chamber bottom Hole Needle location Fig 9 CRP R2 needle location A Becareful to be out of small hole on bottom of CRP R2 chamber Press the Enter key needle moves up and carriage returns to home position Adjustment value is displayed on Tube number WBC
81. e assay values are within the allowable range RAS259 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 4 CRP Calibration A Before starting CRP calibration make sure that the 3 CRP reagent sensitivity factors correspond to the reagents To change those factors please refer to 4 3 Input of the CRP reagent sensitivity factors page 6 4 1 Calibrating the CRP mode Make sure the printer is ON Select the CBC CRP mode using the Mode key Obtain the ABX CRP Std serum and let it equilibrate to room temperature Rotate the tube holder so that the calibrator vial will be in the 12 00 o clock when it is placed into tube holder for sampling Verify that there are sufficient quantities of CRP reagents before running calibration Caution You must remove the caps from all samples before placing them into the tube holder Failure to remove the caps will cause severe damage to the sample needle assembly Remove the cap from the vial place the calibrator into the tube holder and close the tube holder door The analysis cycle will take approximately 4 minutes 30 seconds Results will print at the end of the cycle Repeat previous steps for 5 to 7 cycles When all CRP calibration analyses are complete obtain all the printed results and calculate the Mean X Standard Deviation SD and Coefficient of Variation CV for the following parameter CRP lt 5 0 The calibration is acceptable if a
82. e displayed in the sample N display unit 2 Take proper action after referring to paragraph Error codes further in this Chapter Failed to close the sample holder and it opened The sample needle has stopped in the process of absorption If the holder is closed in this state the needle will be damaged When the Ready LED lights up in about 50 seconds after pressing the Start up Pause key measurement can be performed again 7 2 ABX Micros CRP 200 Technical Manual RAA028A 2 Trouble displayed by code Alarm amp error list Trouble displayed by code If the instrument detects trouble by self diagnosis the error code is displayed on the sample N display unit Some errors are printed out as a message Printed error messages Error Cause code E1 E2 E3 E4 E5 E7 E8 E9 1 Startup failed Indicates that the blank value from self diagnosis after turning ON the power is not within the specified value Content of the error and counter measures are the same as those for E15 E24 and E27 2 Temperature error This is printed when the temperature of the ABX Minidil LMG is not within the temperature range of the measurement specifications Use the instrument and reagents in the temperature range of 15 to 30 C Vacuum syringe does not return to home position in the specified time frame CBC Liquid syringe does not return to home position in the specified time frame Sample needle carriage does not
83. e key is pressed by mistake during the entering of the sensitivity factors the CRP display blinks and the instrument becomes ready for measurement If the ABX CRP REA kit is not placed or deteriorated the CRP display blinks in the Ready state after completion of calibration The measurement can be performed however the reliability of the measurement value will be lost due to the lack of calibration thick line below the result and CRP REAG VALUE UNRENEWED will be printed in the printed CRP results see below Exemple of display ABX micros CRPaco example of printout UNRENEWED is printed out 2 CRP REAG VALUE UNRENEWD RBC PLT Flags WBC 10 3 10 4L MCV e CYCLE HGB En REC 4 85 10S2uL MCH HGB 15 7 s dL e READY HOT GRE 47 9 RDW PLT 271 1097eL MPV O c sus 172 PDW e ROR CRP 0 1 91 Thick line is printed out Blinks 4 Carry out the calibration Run F37 function to print the configuration and check the blank CRP value It must be less than 0 2 ABX Micros 200 Technical Manual RAAO28A RAS259 7 RAS259C RAS259 8 ABX Micros 200 Technical Manual RAA028A Micros 200 RAS260C Step by step analysis cycle Concerns Analysis cycle Required tools None Required products None Intervention time None Frequency On request Specific kit or consumables None
84. eagent cooling unit Similarly be sure to remove the cap of CRP R2 and set it in the cold room Slowly set CRP R1 in the R1 reagent holder door 2 CRP R2 Automatic open close cover Reagent protection cover Reagent cooling unit p This instrument is not provided with a cap detecting function Therefore be sure to remove the CRP 1 CRP 2 and CRP 3 reagent bottle caps before setting the bottles in the instrument reagent should be refrigerated 2 to 10 C 35 6 to 50 F storage in a freezer is prohibited After completion of measurement take the reagent out of the reagent cooling unit tighten the cap firmly and store in a refrigerator Be careful not to misplace the cap Set the reagents in the instrument slowly If the reagent container is dropped in the reagent cooling unit the liquid will spill so care must be taken 4 After the reagent is set be sure to close the reagent door CRP 1 is stable even at room temperature Accordingly it is designed not to be placed in the reagent cooling unit However it should be stored in the refrigerator the same as the other reagents RAS256 6 ABX Micros 200 Technical Manual RAAO28A Micros 200 3 4 Installing the waste liquid tank A Waste liquid may contain cyanide Handle it carefully Never ingest the waste liquid as it may contain biological residual substances infectious substances Always follow the recommend
85. econds to complete the reaction between buffer and CRPproteins 4 2 4 During CRP Preparation 2 Incubation Needle moves over WBC chamber WBC chamber is drained Carriage goes right Valve 12 Vacuum syringe goes up Curves are printed out RBC chamber is drained Valve 11 Valve 13 Vacuum syringe goes up Rinsing of WBC chamber with diluent Liquid syringe goes up inject 2 6ml of diluent into WBC chamber Draining Needle moves to RBC chamber Valve 2 Carriage right over RBC chamber Sampling needle carriage goes down Vacuum syringe draining and rinsing of RBC chamber with diluent Valve 5 Vacuum syringe initialization Valve 11 Liquid syringe goes up inject 2 6ml of diluent into RBC chamber Initializations and needle rinsing block draining Liquid syringe initialization Sampling needle carriage initialization Vacuum syringe goes up Valve 6 Valve 4 Liquid syringe goes down Valve 8 Carriage goes left Graphic printout RAS260 8 ABX Micros 200 Technical Manual RAAO28A Micros 200 2 5 CRP Measurement Creation of a 4pl air bubble in the needle and sampling of 200ul of CRP R3 reagent latex CRP reagent flap is opened Liquid syringe goes down aspirate 4ul of air in the needle Sampling carriage goes down into CRP R3 reagent bottle Valve 15 CRP syringe goes down 100 of CRP R3 sampling N
86. ed procedures for waste disposal Never connect the instrument wastes directly to the laboratory drain pipes For each waste container follow the neutralization procedure See User manual Section 7 Maintenance and troubleshooting Connect the waste container using the cristal tube 3x6 on the waste output and place the waste container below the instrument level under the bench Waste E SY A Insert the other side of the waste tube deeply into the cap head of the attached waste container Remove the cap of the waste container and mount a cap with waste tube Waste liquid tank is not provided with an overflow detecting function When the quantity of waste liquid exceeds half of the tank dispose of the liquid See User manual Section 7 Maintenance and troubleshooting 4 Be sure to use waste liquid tank cap If the tube is directly inserted into the waste liquid tank the pressure will cause the instrument to malfunction Tighten the screw of connector firmly Install the waste liquid tank at the same height as or lower than the main body Use an attached waste tube 3x6 1 5m Do not bend it Do not apply force to the joint of the tube connecting block 3 5 Wiring between the power source and the ground To avoid electric shock connect the power cable only to a hospital grade socket outlet Check if the power switch on the left side of the instrument is OFF Insert the attached power cable into the socke
87. eedle goes up and moves to CRP chamber Vacuum syringe goes up Valve 11 Valve 8 Liquid syringe goes up Sampling needle carriage goes up Carriage goes right over CRP chamber Needle goes down into CRP chamber and vacuum syringe is initialized Sampling needle carriage goes down into CRP chamber Valve 5 Vacuum syringe initialization Valve 2 Distribution of CRP R3 sample into CRP chamber and CRP chamber dilution mixing Valve 15 CRP syringe goes up inject CRP R3 reagent into CRP chamber Sampling needle carriage goes up Valve 14 CRP syringe goes up amp down several times mixing It takes 20 seconds to complete the reaction between latex and CRP proteins EM CRP measurement starts for 80 measures per second 2 6 CRP After Cycle chamber draining and cleaning with diluent from inside and outside needle Valve 18 Vacuum syringe goes up Sampling needle carriage goes down into CRP chamber Valve 14 CRP syringe initialization Valve 11 Liquid syringe goes up Valve 10 Liquid syringe goes up Valve 18 Vacuum syringe goes up Previous step is repeated one time Needle out from CRP chamber Vacuum syringe goes up Valve 8 Sampling needle carriage initialization Carriage initialization CRP blank is performed CRP result is printout ABX Micros 200 Technical Manual RAAO28A RAS260 9 RAS260C RAS260 10 ABX Micr
88. ensor Run 2 Diluent prime F 13 functions Enter F 68 function Temperature check See Fig 2 F68 display page 2 ABX MiCPOS CRPeoo WBC EHI CYCLE HGB EE READY HCT Em BUSY ERROR Fig 2 F68 display Temperature without correction is displayed on WBC display Corrected temperature is displayed on RBC display Check temperatures between RBC display and thermometer then press Enter RAS265 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 2 Diluent temperature in CRP chamber check Remove the instrument cover Plunge thermometer probe deeply into CRP chamber See Fig 3 CRP chamber temperature page 3 Fig 3 CRP chamber temperature Instrument must be switched on for about 15min at least to reach its correct temperature Run F 96 function Cooler Heater check See Fig 4 F96 CRP diluent page 37 ABX ITli COS CRPaoo Fig 4 F96 CRP diluent The WBC and RBC displays must be blinking between 0 and 1 Check temperature on thermometer temperature must be between 36 C 97 F and 38 100 F Fig 5 R66 on CRP board ABX Micros 200 Technical Manual amp AA028A RAS265 3 RAS265C 3 CRP reagents temperature check Remove the instrument cover Plunge thermometer probe between CRP reagent bottle and CRP block See Fig 6 CRP reagent temperature page 4 Fig 6 CRP reagent temperature 4 Instrume
89. ent sensor 2 view page 7 3 H1008152003 CABLE LYSE SENSOR CRP200 4 Liquid syringe view page 8 1 F0020373000 O RING DILUENT PISTON CRP200 4 Liquid syringe view page 8 2 FAA055A O RING MICROS SAMPLING SYRINGE 4 Liquid syringe view page 8 3 FAA036A O RING FLOW CELL LYSE DISP MIC 4 Liquid syringe view page 8 4 G0166380 SYRINGE 3 SYR BLOCK ASSY CRP200 4 Liquid syringe view page 8 5 H1008301001 O RING TEFLON 12uL SYRINGE 200 4 Liquid syringe view page 8 6 H1008302001 SYRINGE DIL BLOCK BODY CRP200 4 Liquid syringe view page 8 7 H1008303001 SYRINGE DILUTION BLOCK COVER 4 Liquid syringe view page 8 8 H1008304002 SYRINGE DILUENT PISTON KELF 4 Liquid syringe view page 8 9 H1008305001 SYRINGE LYSE PISTON CRP200 4 Liquid syringe view page 8 10 H1008331001 NEEDLE SAMPLE DISPENSER CRP200 G0166381 SYRINGE CRP SYRINGE ASSY CRP200 H1008322001 SYRINGE CRP SYRINGE BODY CRP200 H1008323001 SYRINGE CRP BOTTOM PLATE CRP200 H1008305001 SYRINGE LYSE PISTON CRP200 5 CRP syringe view page 9 5 CRP syringe view page 9 5 CRP syringe view page 9 5 CRP syringe view page 9 5 CRP syringe view page 9 FAA036A O RING FLOW CELL LYSE DISP MIC 6 Sampling needle rinsing block view page 10 FAA053A O RING SAMPLING NEEDDLE MICROS OT 6 Sampling needle rinsing block view page 10 GBCO69AS NEEDLE SAMPLING MICROS OT LC 6 Sampling needle rinsing block view page 10 DBK019A CLIP SAMPLING NEEDLE HOLDER 6 Sampling needle rinsing block view page 10 H1007659001 NEEDL
90. er See Fig 6 Needle on WBC chamber page 4 Fig 6 Needle on WBC chamber Press ENTER Needle moves up and carriage returns to init position number of steps is returned into WBC diplay check value is between 612 and 639 RAS261 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 4 Carriage location over WBC RBC chamber adjustment 4 1 Adjustment Locate centering tool GBF055A over the RBC and WBC chambers See Fig 1 needle location page 2 Fig 7 Centring tool Enter F 54 function press ENTER Carriage is moved over WBC chamber Manually pull down needle inside WBC chamber Hit a key to continue needle moves up and carriage returns to home position Adjustment value is displayed between Tube number and WBC displays Write down this value and press ENTER to exit function This value is Carriage value in configuration ticket 4 2 Adjustment Check Enter F 53 function press ENTER Carriage is moved over WBC chamber and needle is coming down into it Check that the needle is in centering tool and press ENTER Carriage is moved over RBC chamber and needle is coming down into it Check that the needle is in centering tool and press ENTER If needle is not well centered enter F 55 function and press ENTER Needle is too much backward decrease CARRIAGE value by the meaning of Up and Down keys 1 step for is too much forward increase CARRIA
91. es between the two electrodes As the blood cells pass through the aperture they create resistance Impedance in the electronic field between the two electrodes The voltage which measures the cells is proportional to the size of the cell Since the current is constant and remains unchanged the larger the cell is the more resistance it has The smaller the cell is the less resistance it has These electronic voltages vary in pulse size as the cells pass through the aperture The pulses are amplified channeled according to size and threshold grouped and then mathematically calculated along with the calibration coefficients to give a final numerical value for both RBC s and PLT s Results Number of cells counted per volume unit x calibration coefficient Histograms RBC Distribution curves on 256 counting channels from 30fl to 300fl PLT Distribution curves on 128 channels from 2fl to a mobile threshold This threshold moves according to the microcyte population present in the analysis area 5 4 ABX Micros Fig 1 Impedance principle RBC pulse PLT pulse Number of cells Analogue convertion Cell volume Number of cells Data integration and plotting of RBC distribution curve Cell volume Fig 2 RBC distribution curve Number of cells Analogue convertion Cell volume Data integration and plotting of PLT distribution curve Number of cells Cell vp
92. he needle motion value is more than 3 digits the fourth digit is displayed in N window Check for each tube holder position the value is in the following limits Position 2 Limit values mini 584 maxi 606 Position 4 Limit values mini 1029 maxi 1043 Position 5 Limit values mini 644 maxi 665 Position 6 Limit values mini 931 maxi 952 RAS261 8 ABX Micros 200 Technical Manual RAAO28A Micros 200 Position 5 Position 2 Position 4 Position 6 Switch 2 Sampling position Bad position of the tube holder 0 1 0 Position 2 0 0 1 Position 4 1 0 1 Position 5 0 1 1 Position 6 1 1 1 No tube holder Enter F 82 function to check correct adjustment Press Enter move by hand the needle It must not touch the bottom of the hole Repeat this operation for each position Specific adjustment for control blood position If needle touchs the bottom of the control blood vial or if the control blood vial is not well emptied perform the following adjustment Theorical level for position 2 adjustment Enter F 81 function to adjust control blood tube holder s position 2 Install an empty vial in position 2 of the tube holder close the tube holder and press Enter the tube holder position is displayed Manually move the needle down inside the vial then press Enter the needle moves up and the value is displayed Write down this value and enter F 84 function Into F 84
93. here ABX Micros 200 Technical Manual RAA028A 2 7 ABX Micros CRP 200 3 5 Right side view Automatic open close cover Reagent protection cover Reagent cooling unit Reagent setting position Set the reagents ABX CRP REA CRP R1 CRP R2 and CRP R3 in the instrument as illustrated Reagent door 2 Turn the button to open the door Reagent protection cover This cover prevents evaporation of reagent and dust from getting into reagents Automatic open close cover Automatically opens closes during measurement and primes the reagents separately Reagent cooling unit Keeps the reagent ABX CRP REA CRP R1 CRP R2 and CRP R3 cold Do not open the reagent door 2 during measurement and during cleaning If the reagent door 2 is opened measurement and cleaning stop By closing the door the operation continues But if the door is opened or closed during measurement the reliability of the measured values will be lost so please be careful 2 8 ABX Micros 200 Technical Manual RAA028A Specifications ABX Micros CRP 200 description 3 6 Sample tube holder Position 1 Measurement position Position 4 Position 2 Position 3 A me c Position 1 913mm x 81mm for normal sample tube for example Venoject II Nipro neo tube etc Position 2 98mm x 45mm for a small sample tube 4 Position 3 918 8mm x 35mm for the control blood bottle and calibration serum
94. icros CRP 200 1 2 Functions of valves Valve blocks are located close to concerned elements Two different blocks Valves 1 2 8 10 11 14 15 on front side horizontal block behind front panel See Fig 1 valve block 1 page 4 E ES O 1 Fig 1 valve block 1 Valves 4 5 6 7 12 13 18 on right side horizontal block under WBC RBC Chambers See Fig 1 valve block 1 page 4 Fig 2 valves block 2 Lyse Control Entry Exit syringe LV2 Vacuum syringe Set to atmosphere LV4 Cleaner Counting heads cleaning LV5 Waste Control Entry Exit LV6 Drain Counting heads LV7 Diluent Directing Rinsing block Counting heads LV8 Diluent Draining of rinsing block 3 4 ABX Micros CRP 200 Technical Manual RAA028A Hydraulic amp Pneumatic principles Hydraulic 1 10 Diluent Directing Sampling syringe Valve 7 1 11 Diluent Control Entry Exit syringe 1 12 WBC chamber Draining LV13 RBC chamber Draining LV14 CRP chamber Directing CRP chamber Valve 15 LV15 CRP syringe Control Entry Exit syringe 1 18 Draining ABX Micros CRP 200 Technical Manual RAA028A 3 5 ABX Micros CRP 200 2 Pneumatic diagram 2 v buimelg l 2 2 N 002661083 4 id YJ4WYH9 HasiNvHO S
95. ing precautions Carry out previously an autoconcentrated cleaning to make sure of the cleanliness of the RBC PLT counting circuit If necessary clean the RBC PLT chamber aperture as described in the procedure CHAMBER MAINTENANCE of this manual Do not operate gain adjustment as long as the RBC and PLT values are not stable Make sure the Latex has been thoroughly mixed before Remove the MICROS CRP cover Switch on instrument Run F 65 function RBC PLT Latex Adjust This function allows the dilution of latex in diluent and displays RBC and PLT volumes on screen every 3 seconds Value blinking in WBC display shows the RBC target depending on the Latex lot used usually 74 1 if necessary you can change this value by the mean of Up and Down keys Press Enter Value blinking in RBC display shows the PLT target depending on the Latex lot used usually 64 1 if necessary you can change this value by the mean of Up and Down keys Press ENTER the instrument is ready to sample RBC PLT latex Locate Latex vial in tube holder and close sampling door cycle duration 3min WBC and RBC displays show the actual measurement of the latex volume Adjust RBC gain by means of R81 potentiometer on mother board and PLT gain by means of R87 potentiometer See Fig 1 Mother board page 2 At the end of the cycle average volumes and distribution curves are printed out RAS262 10 ABX Micros 200 Technical
96. ion Required tools None Required products Fresh and normal blood samples Calibration blood samples CRP Calibration serum Intervention time eth Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS259C RAS259 2 This procedure must be performed on a clean instrument If instrument is suspected to be not perfectly clean perform a CLEAN cycle 1 Preliminary Run a STARTUP cycle 2 Repeatability Based 10 consecutive analyses on CBC and 5 consecutive analyses on CRP without alarm from one fresh and normal blood sample Run 10 consecutive analyses on CBC and 5 consecutive analyses on CRP Control to have variation coefficients within the following acceptable limits Parameters CV WBC 2 5 RBC 2 HGB 1 7 HCT 2 PLT 5 MCV 1 RDW 3 MPV 3 LYM lt 5 MON lt 10 GRA lt 3 CRP lt 10 CRP lt 4 X Mean Xi Measure value n Measure number SD Standard deviation rt CV is calculated by means of the below formula 37 0 150 83 12 18 5 2 5 49 Measurement range 12 50 x 10 mm 5 00 x 10 mm 16 6 g dL 50 0 355 x 10 mm 100 fL 15 9 fL 47 0 6 0 78 lt 0 5 mg dL lt 5 mg dL CV 22 100 X ABX Micros CRP 200 Techni
97. l Manual RAA028A Exploded views 17 Carriage view 1 DBEO14A CABLE BUSHING D 3 2 BLACK 2 F1000391700 BELT NEEDLE 285MM CRP200 3 F1000391800 BELT CARRIAGE 650MM CRP200 4 G0166610 MOTOR STEPPER CRP200 5 G0166620 SENSOR NEEDLE CARRIAGE CRP200 6 H1008358001 MOTOR PULLEY NEEDLE CRP200 7 G2002319 KIT PULLEY ASSY CRP200 ABX Micros CRP 200 Technical Manual RAA028A 9 21 ABX Micros CRP 200 18 CRP cold assy view 1 DAMOO7A VALVE SOLENOID CRP BLOCK 2 H2003841001 REAGENT SILICON STOPPER CRP200 3 G2002533 CHAMBER CRP COLD ASSY CRP200 9 22 ABX Micros 200 Technical Manual RAA028A Exploded views 19 Reagent sensor 1 view 1 G2002318 SENSOR DILUENT SENSOR CRP200 2 H1008152001 CABLE DILUENT SENSOR CRP200 ABX Micros 200 Technical Manual RAA028A 9 23 ABX Micros CRP 200 20 Air syringe cup view 1 GBF070A CUP OVERFLOW AIR SYRINGE LC270 9 24 ABX Micros CRP 200 Technical Manual RAA028A Exploded views 21 Air syringe view 1 W FAA017A GBC260AS G0166320 H1008317001 H1008355001 H1008373001 H2003334001 008 O RING TANK MIN AG WASTE MIC CHAMBER INJ WASTE VAC SYR BODY SENSOR VAC WASTE SYRINGE CRP200 CHAMBER WASTE VAC SYR PISTON SYRINGE COGGRAIL FOR AIR SYR SENSOR OPTIC DETECT TAB CRP200 CHAMBER WASTE CHICANE CRP200 SYRINGE PLASTIC GUIDE L 63 5 ABX Micros CRP 200 Technical Manual RAA028A 9 25 ABX Micros
98. logy Measuring principles Prozone After the first 20 seconds of the reaction if the absorbance is over the absorbance threshold the CRP display window will display EE E and P on the printed report Readings in the Prozone area should not be considered accurate If this flag appears on the printed report dilute the sample and re analyze it If the diluted result is still abnormal other laboratory reference methods may be needed to obtain an accurate CRP result on these types of specimens CRP FLAGGING AREAS Prozone ABSORBANCE at 20 Seconds 0 5 10 15 20 25 30 CRP mg dL 1 10 7 CRP final calculation The final CRP concentration results is determined by the following formula curve x Calibration Coefficient x CRP Reagent Sensitivity Factor x HCT coeff CRP Curve this is the slope data obtained during the antigen antibody reaction counting period CRP Calibration Coefficient this is the value obtained when performing the CRP Calibration CRP Reagent Sensitivity Factors those factor numbers are noted on each ABX CRP REA reagent kit Coefficient this is the Hematocrit value obtained from a whole blood sample or from the conversion table when running whole blood CRP dilutions ABX Micros 200 Technical Manual RAA028A 5 11 ABX Micros CRP 200 5 12 ABX Micros CRP 200 Technical Manual RAA028A Software versions CONTENTS 1 S
99. lume Fig 3 PLT distribution curve 200 Technical Manual RAA028A Analysis cycle technology Measuring principles Technical characteristics for the RED BLOOD CELL and PLATELET count Dilution First dilution volume used 30 ul Vol ABX DILUENT 2500 pl Final dilution rate 1 15000 Measurement Method Impedance Ruby diameter 50um Depression of count 200mb Duration of the count 2x5 sec Results Number of cells counted per volume unit x Calibration coefficient 1 3 Hemoglobin measurement principle During the Startup cycle an HGB blank test sequence including 2 blank measures is run If the difference between these two measures is too important a third measure is performed reference blank sequence will be carried out prior to an analysis to come If the operator ET Has left system more than 10 minutes after analysis Has not carried out the Startup cycle after switching on the system Every cycle an HGB blank is carried out on diluent and compared to the previous HGB blank analysis Lyse reagent is added to the first dilution in the WBC HGB chamber Lyse This reagent contains potassium ferricyanide Fe CN K and potassium cyanide KCN The hemoglobin freed by the lysis of the red blood cells combines with the potassium cyanide to form the chromogenous cyanmethemoglobin compound The compound is then measured by spectrophotometry through the optical part of the WBC HGB chamber with a wave length of 550
100. lve 11 Valve 2 Valve 7 Valve 6 Liquid syringe goes up diluent for 1 2ml WBC chamber and vacuum syringe draining movement of lyse back in the tubing near the mixing T Valve 12 Vacuum syringe goes up Draining Valve 5 Vacuum syringe initialization Valve 1 Sampling needle carriage goes down Liquid syringe goes down ABX Micros 200 Technical Manual amp AAO028A RAS260 5 RAS260C Air drawing inside the needle and down into WBC chamber Liquid syringe goes down Sampling needle carriage goes down Valve 11 Liquid syringe goes up diluent for 1 54ml Draining Valve 2 WBC chamber draining and loaded with diluent RBC amp WBC chamber draining rinsing block cleaning Valve 12 Vacuum syringe goes up Liquid syringe goes down Draining Valve 2 Valve 11 Liquid syringe goes up Valve 5 Vacuum syringe initialization Draining Valve 13 Vacuum syringe goes up Draining Valve 12 Vacuum syringe goes up Draining Valve 8 Sampling needle carriage goes down First dilution and bubbling Valve 2 Valve 11 Vacuum syringe goes down Liquid syringe goes up 0 5ml outside needle Valve 10 Liquid syringe goes up 1 2ml of blood and diluent inside needle Valve 12 Vacuum syringe goes down and up Vacuum syringe goes down and up Needle and liquid syringe initialization rinsing block cle
101. ly Wai 9 15 12 Cooling fan 9 16 13 Reagent connection plate Vie Weiss 9 17 9 18 15 Display board 9 19 16 Syringe mechanical 9 20 TZ 9 21 18 CRP cold assy view n 9 22 T9 Reagent sensor 9 23 20 Air syringe CUD VOW 9 24 2 L ME Syringe VIEW 9 25 22 Main board VIEW M CSS 9 26 23 Front panel view o A en 9 27 24 Sample holder VAGA 9 28 25 CONECT WOW V 9 29 26 Back T 9 30 27 Keyboard assy view ad iia 9 31 28 C RP UNI MIGW TT n 9 32 29 Right side O 9 33 30 Maintenance kit view aigue pd 9 34 IE Via 9 35 32 Straw and tubing VIBW apa 9 36 33 Waste container VA 9 37 ABX Micros CRP 200 Technical Manual RAA028A 9 1 ABX Micros CRP 200 1 List Fo E drawing 2 CRP board view page 6 E0012926700 PCB BATTERY ASSY CRP200 2 CRP board view page 6 2 61001410 PCB CRP BOARD CRP200 2 CRP board view page 6 B E1000349100 CABLE CRP DISPLAY CRP200 2 CRP board view page 6 4 E1000334900 ADHESIVE FERRITE CRP200 2 CRP board view page 6 5 E1000348900 CABLE LIQ VALVE BLOCK 8 10 CRP 3 Reagent sensor 2 view page 7 1 G2002320 SENSOR REAG SENSOR 2 WAY CRP 3 Reagent sensor 2 view page 7 2 H1008152002 CABLE CLEANER SENSOR CRP200 3 Reag
102. ment Check between TP38 and TP31 Ground CRP syringe motor voltage If necessary adjust CRP syringe motor voltage by R168 potentiometer on mother board See Fig 1 Mother board page 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 3 5 Needle Carriage No adjustment required for needle carriage motor 4 Pressure sensor adjustment Target 1 7V 0 1 Remove the MICROS CRP cover 4 Switch on instrument 4 Disconnect the second tubing from the top of the vacuum syringe See Fig 2 vacuum syringe page 5 Fig 2 vacuum syringe Check CRP board between TP16 and TP6 Ground Pressure sensor voltage If necessary adjust Pressure sensor voltage by VR2 potentiometer CRP board See Fig 1 Mother board page 2 A The adjustable range of VR2 is 270 degrees Attempting to turn it in excess will damage VR2 ABX Micros 200 Technical Manual RAAO28A RAS262 5 RAS262C RAS262 6 5 Power supply voltages check adjustment available for power supply voltages 4 5 1 Power supply 12 V Target check 12V 0 5 Remove the MICROS CRP cover Switch on instrument Check between TP20 and TP31 Ground 12V Power suply voltage See Fig 1 Mother board page 2 5 2 Power supply 12 V Target check 12V 0 5 Remove the MICROS CRP cover Switch on instrument Check between 21 and TP31 Ground 12V Power suply voltage
103. movement guide rail 2 Power OFF Wait 10 seconds Power ON and press the Startup Pause key to run Startup Power OFF Wait 10 seconds Power ON and press the Startup Pause key to run Startup 1 Replace the sample needle 2 Power OFF Wait 10 seconds Power ON and press the Startup Pause key to run Startup 1 Complete the function or cycle and then press the Reprint key 2 Turn the printer power ON Restart sequence after 30 seconds or press the Start Pause key to reset error Turn the printer On ABX Micros CRP 200 Technical Manual RAA028A ABX Micros CRP 200 code E10 E11 E12 E13 E14 E15 E16 E17 E19 7 4 Printer does not accept print signal printer is busy Printer does not accept print signal No is selected for printout format by F36 Printer does not accept print signal printer is turned off or is not connected The instrument does not recognize the position of the sample tube holder Position of the sample holder can not be recognized correctly Blank value is out of range WBC PLT Sample holder is open Sample holder does not open Startup unexecuted The printer cable is disconnected when a result should be printed out or when the Reprint key is pressed No printer has been selected in F36 print format selection 1 The printer cable is disconnected when a result should be printed out or when the Reprint key is pressed 2 The p
104. n Vacuum syringe draining Valve 5 Vacuum syringe initialization Valve 2 Liquid syringe loading Liquid syringe down Carriage goes left Vacuum syringe up Vacuum for the first counting about 200mb Vacuum syringe goes up Valve 6 Counting heads cleaning with diluent Valve 7 Valve 11 Liquid syringe goes up Counting 2 3 CRP Preparation 22 Needle draining and movement over CRP R2 reagent bottle CRP reagent flap is opened Valve 8 Vacuum syringe goes up Sampling needle carriage goes left over CRP R2 reagent bottle Creation of an air bubble into needle Valve 2 Vacuum syringe initialization 100 yl CRP R2 reagent CRP buffer sampling Sampling needle carriage goes down Valve 15 CRP syringe goes down 100 of CRP R2 sampling ABX Micros 200 Technical Manual RAAO28A RAS260 7 RAS260C Needle up and cleaning carriage moves over CRP chamber Vacuum syringe goes up Sampling needle carriage goes up Valve 8 Carriage right over CRP chamber CRP R2 reagent mixing in CRP chamber Valve 2 Vacuum syringe initialization Sampling needle carriage goes down into CRP chamber Valve 15 CRP syringe goes up inject CRP R2 reagent in CRP chamber Valve 14 CRP syringe goes up CRP syringe goes down CRP syringe goes up Sampling syringe goes up CRP reagent flap is closed It takes 20 s
105. n board and PLT gain by mean of R87 Two bubbling phasis are adjustable 130 0 20 Bubbling 1 is the first dilution WBC HGB chamber bubbling value Bubbling 2is the second dilution WBC HGB chamber lyse bubbling value do not change More than 1 hour after turning ON the Value power run F13 twice Prime Diluent then run F67 T is displayed on WBC display By means of Up and Down keys input the T read on thermometer then press the Enter key ABX Micros CRP 200 Technical Manual RAAQ28A 70 5 Value GRA PLT 100 0 10 6 7 ABX Micros CRP 200 ee e F68 Temperature T check Run F13 twice Prime Diluent then Not calibrated Calibrated See RAS265 Thermic run F68 As calibration 2 C is adjustment automatically added to the T input for T adjustment T without correction is displayed on WBC display Corrected T is displayed on RBC display Check T between RBC display and thermometer then press the Enter key F69 Pressure check Connect a Barflex to the secong input See RAS263 Vacuum of the Waste Vacuum syringe Press the check Enter key Vacuum must be within the following range from 220 to 190 mb Wait for 30s the vacuum drop down must be less than 2 mb HGB blank adjustment values Channel Temperature room Nominal 15 240 245 250 16 240 245 250 17 239 244 249 18 238 243 248 19 237 242 247 20 236 241 246 21 235 240 245 22 234 239 244 23 234 239 244 2
106. nd only if it comes within the Calibration Passed criteria 4 2 Calibration Passed Criteria The calibrated parameter must be within it s CV limits The calculated Mean value of the parameter must be within 20 of the target value indicated on the calibration assay sheet Note If calibration does not fall within these criteria then calibration has Failed Press the Calibration key located on the top key pad Now press the Reprint key The old calibration coefficients will be now be printed out The WBC coefficient will be flashing in the WBC parameter window Press the Enter key several times to scroll down to PLT Continue to press the Enter key to scroll past the second WBC and RBC values until the CRP window flashes a value Calculate the new calibration coefficient for CRP using the following formula ABX Micros 200 Technical Manual RAAO28A RAS259 5 RAS259C Target Value from assay sheet New calibration coefficient x Old cal coefficient Mean parameter value from calculations Adjust the CRP coefficient by using the Up or Down arrow keys then press the Enter key several times to accept the change and exit the calibration menu Verify that your new calibration coefficient is within the ranges listed before Now run the calibrator 3 times and verify that the average of all three results are within the limits that are noted on the assay sheet 4 3 Input of the CRP reage
107. nm Technical characteristics for the measurement of the hemoglobin Dilution Initial blood volume 10 ul Vol ABX DILUENT 2500 pl First dilution rate 1 250 Vol Lyse 520yl Second dilution rate 1 300 Measurement Method Photometry Wavelength 550nm Result Absorbance value obtained x coefficient of calibration 1 4 Hematocrit measurement principle The height of the impulse generated by the passage of a cell through the micro aperture is directly proportional to the volume of the analyzed RBC The hematocrit is measured as a function of the numeric integration of the MCV ABX Micros CRP 200 Technical Manual RAA028A 5 5 ABX Micros CRP 200 1 5 RDW calculation The RDW Red cell Distribution Width is used to determine erythrocyte abnormalities linked to Anisocytosis The RDW will enable the user to follow the evolution of the width of the curve in relation to the cell number and average volume The RDW is also a calculation from the RBC histogram as follow KSD system constant SD Determined standard deviation according to statistical studies on cell distribution MCV Mean Corpuscular Volume of erythrocytes 1 6 MCV MCHC calculation MCV Mean Cell Volume is calculated directly from the entire RBC histogram Mean Corpuscular Hemoglobin is calculated from the HGB value and the RBC count The Mean HGB weight in each RBC is given by the formula MCH pg HGB RBC x
108. nt Thermic adjustment Front panel dismantling Autoconcentrated cleaning CRP unit replacement Concerns Unpacking Micros CRP 200 installation Reagent priming Startup Instrument decontamination before maintenance Yearly maintenance Check up after intervention Described the analysis cycle step by step Needle amp carriage position adjustment Main amp CRP boards adjustment motor voltages thresholds gains etc Vacuum check Bubbling check Thermic adjustment Cover amp front panel dismantling Bleach cleaning CRP unit replacement ABX Micros CRP 200 Technical Manual RAA028A 8 3 ABX Micros 200 8 4 ABX Micros CRP 200 Technical Manual RAA028A Micros 200 RAS256C Installation Concerns Installation kit Reagents installation Priming and startup Required tools Hexagonal keys Required products MICROS CRP Reagents Bottles Waste container Intervention time 1 hour Frequency Specific kit or consumables nstallation kit XEA911A Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS256C 1 Installation kit XEA911A DARO14A Fuse 5x20 3 15A T 250V 2 EAE007A Tygon tube 1 52mm 0 060 2ML EAE009A Tygon tube 2 29mm 0 090 2 ML FAA017A O RING TANK MIN AG WASTE MIC 1 F0020373000 O ring 15x1 5 NIT 70SH 1 FAA036A O RING FLOW
109. nt must be switched on for about 15 min at least to reach its correct temperature Run F 96 function Cooler Heater check See Fig 7 F96 CRP reagent page 4 ABX Ini COS CRPaoo RBC CYCLE HGB 277 READY BUSY ERROR Fig 7 F96 CRP reagent A The WBC and RBC displays must be blinking between 0 and 1 RAS265 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 RAS268C Front panel dismantling Concerns Front panel Required tools Hexagonal keys Dynamometric screw driver A302 A301 A300 Flat screw driver Torx keys Required products None Intervention time 10 mn Frequency On request Specific kit or consumables None worn by the operator Local or national regulations must be applied in all the operations 4 Disposal gloves eyes protection and lab coat must be RAS268C 1 Front panel dismantling Switch off the instrument and disconnect the power supply cable Open the front reagent door and remove the 2 bottles disconnect tube on the stopper Unscrew the 6 CHC cover screws with their washers See Fig 1 cover screws page 2 then remove the cover Fig 1 cover screws Unscrew the 2 CHC M2 5x5 screws under the frame See Fig 2 below screws page 2 Fig 2 below screws Push the carriage back and unscrew the 4 CHC M2 5x8 screws See Fig 3 Inside screws page 2 E Fig
110. nt sensitivity factors The CRP reagent sensitivity factors are described on the underside of the colored box lid of ABX CRP REA In order to perform lot correction of the reagent be sure to enter those factor values in the instrument every time the lot changes Select the CBC CRP mode Push PUSH on the top front of the main body to open the upper door Press the Calibration key The WBC result display starts blinking The WBC calibration coefficient currently stored is displayed Press the Reprint key to print out the CRP reagent sensitivity factor CRP factor currently stored COEFFICIENTS DATE 04 01 2005 TIME 16 37 WBC 1 10 0 84 HGB 1 27 HCT 1 03 Calibration PLT 1 17 coefficients MPV 0 90 RDW I 0 81 CRP I 1 08 CRP FCT A 0 90 7c CRP Reagent CRP FCT B 1 04 lt sensitivity factors CRP FCT C 0 8749 CRP BLANK 14 12 2004 Renewed date amp time 11 30 or UNRENEWED 2 Inthe USA PCT and PDW parameters are for research use only and not for use in diagnostic procedures When the Mode key is pressed in the state that the WBC display is blinking CRP reagent sensitivity factor A B and C currently stored are displayed on the HCT PLT and CRP displays Press the Enter key Only the display will start blinking The instrument is now ready to enter the CRP reagent sensitivity factor A Using the Up or Down arrow keys enter the CRP reagent sensitivity factor A ma
111. nually move on the right hand side the carriage and move down the needle until it touch the edge on the right hand side of glass chamber See Fig 8 Needle on glass chamber page 4 Fig 8 Needle on glass chamber Hit a key to continue Needle moves up and carriage returns to home position Adjustment values are displayed WBC display shows CRP needle value RBC display shows CRP carriage value Write down both value and validate to exit function Enter F 57 function press Enter RAS445 4 ABX Micros 200 Technical Manual RAAO28A Micros 200 Carriage is moved over CRP glass chamber and needle is coming down into it Check that needle is well centered into the CRP glass chamber Press Enter for all sequences If needle is not well centered enter F 59 function and press Enter Needle is too much backward decrease CRP carriage value by the meaning of Up and Down keys 1 step for 0 1mm or Needle is too much forward increase CRP carriage value by the meaning of Up and Down keys 1 step for 0 1mm If needle 15 too low or too high enter F 58 function and press Enter Needle is too much down decrease CRP needle value by the meaning of Up and Down keys 1 step for 0 1mm Needle is too much up increase CRP needle value by the meaning of Up and Down keys 1 step for 0 1mm Write down this value and press Enter to exit function Resta
112. oftware OVERVIEW do 6 2 1 1 User menu overview 6 2 1 2 Technician Menus Overview eee ee nennen nens 6 5 ABX Micros 200 Technical Manual RAA028A 6 1 ABX Micros CRP 200 1 Software overview 1 1 User menu overview In order to access to the special functions follow the procedure below Check that the instrument is in ready mode Press and hold the Service key blue Using the Up and Down keys set up the ten number of the required function on the WBC display then press Enter Keep holding the blue Service key and set up the unit number using the Up and Down keys Press Enter when the required unit number is selected Release the blue Service key PRIME CLEAN REPRINT CAL SU Ld ij service Y ENTER F0 Data saved and QC calculation m e SC a FO1 Display all saved data Sample number year hour Year Month Day Hour Minute and minute are displayed first Press the Enter key the results are displayed F02 Printout all saved data saved data of measurement are printed out F03 Printout saved QC CBC data Saved QC CBC data and calculated values AVE MIN MAX CV are printed out F04 Printout saved QC CRP data Saved QC CRP data and calculated values AVE MIN MAX CV are printed out F05 Delete all saved data All the saved data of measurement samples are deleted F06 Delete saved QC CBC data Save
113. or Local or national regulations must be applied in all the operations RAS264C 1 Bubbling adjustment Two bubbling phasis are adjustable Bubbling 1 is the first dilution WBC HGB chamber bubbling value Bubbling 2 is the second dilution WBC HGB chamber lyse value and RBC chamber bubbling value Both values correspond to a number of steps carried out by the vacuum syringe Bubbling range Bubbling 1 Between 150 and 200 Bubbling 2 between 80 and 140 Remove the MICROS CRP cover Switch on instrument 4 Run F 66 function See Fig 1 WBC bubbling display page 2 ABX MiCPOS CRPeoo CYCLE HGB mm v wo A e e e BUSY ERROR Fig 1 WBC bubbling display This value is factory adjusted and may be different from the default values shown above It should be modified only when hematologic erroneous results are given by the instument If bubbling is too important liquid overflows can occur or if bubbling is too low homogeneity of the dilution can be decreased Increase or decrease Bubbling 1 value blinking in WBC display with Up and Down keys Press Enter to display Bubbling 2 value in RBC display See Fig 2 RBC bubbling display page 3 RAS264 2 ABX Micros 200 Technical Manual RAAO28A Micros 200 ABX MiCPOS Fig 2 RBC bubbling display This value is factory adjusted and may be different from the default values shown above It should
114. os 200 Technical Manual RAA028A Micros 200 RAS261C Needle amp carriage adjustment Concerns Needle amp carriage position adjustment Required tools Hexagonal keys Flat screw driver Centering tool GBF055A Required products None Intervention time 30 min Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS261C 1 Carriage sampling location adjustment Remove the ABX Micros CRP cover then switch the instrument on It is mandatory to remove all CRP specific reagents because of risks of vial polution by needle A Put reagents in a fridge for their conservation Enter F 5c function press ENTER Locate tube holder s smallest hole in sampling position A and close sampling door See Fig 1 needle location page 2 Manually move down needle B Move the carrier to adjust the front rear position of the needle in the hole C then press Enter Fig 1 needle location If the position is not correct enter F 5d function Press Enter and re adjust number of step Needle is too much forward Decrease Sampling value displayed on WBC display using Up and Down key one step for 0 1 mm Needle is too much backward Increase Sampling value displayed on WBC display using Up and Down key
115. primed into the instrument required time 3 min and 30 seconds injection Clean key This key is used to clean the measuring cell and tubing of the instrument Reprint key This key is used to send the result of the last measurement to the printer or RS 232C It is also used to print calibration coefficients or limit values Calibration key This key is used to enter the calibration coefficient and CRP reagent sensitivity factor amp Up and Down arrow key Those keys are used to change the numerical values Enter key This key is used to enter the changed numerical values Service key This key is used for maintenance of the instrument and various settings Do not touch this key unless instructed to do so in this manual or by your service representative 2 6 ABX Micros 200 Technical Manual RAA028A Specifications ABX Micros CRP 200 description 3 4 Le t side and rear Fan Printer output RS232 output terminal terminal Diluent amp Waste liquid port Power receptacle Power switch Power switch Placing this switch to ON supplies the power to the instrument Power receptacle Power cable is connected to this receptacle Diluent port ABX Minidil tube is connected here Waste liquid port Waste liquid tube is connected here RS232 output terminal A cable used for an external computer is connected here Printer output terminal Printer is connected
116. r 10 minutes assemblies in contact with the operator such as instrument cover tube holder keyboard start key sampling needle neighboured assemblies 2 Manual decontamination 20 min Remove the chambers cover Dilute the 12 cl bleach to 1 part of bleach for 4 of deionize water 1 5 Instrument environment must be cleaned and decontaminated No sponge nor cloth must be used Only absorbant paper thrown after use in contamination bins can be employed For small or weak assemblies use accurate drier papers All assemblies that is suspected to have contact with biologic product must be disinfected with the diluted bleach the stainless steel must be bleached below 30 Celsius Blood stains or salt marks must be cleaned with spray detergent first Concerned assemblies Outer surfaces of the instrument perpex covers LCD reagent locations Keyboards Waste connector plug Liquid valve push Needle neighboured assemblies Tube holder assy Overflow trays Reinstall all the assemblies and setup the instrument in its initial configuration 3 Analysis circuit decontamination 30 min Prepair 1 bottle containing 1 2 litre of bleach diluted to 1 part of bleach for 9 parts of deionize water 1 10 Prepair 1 bottle containing 1 2 litre of distilled water Switch on the instrument Replace the reagent bottles by the diluted bleach bottle Run a Prime cycle Fill a sample tube with
117. ress the Prime key Reagent ABX Minidil LMG ABX Lyse and ABX Cleaner will be primed into the instrument Repeat previous step three times When priming has been performed three times perform End Cleaning by pressing the Shutdown key and turn off the power RAS256 8 ABX Micros 200 Technical Manual RAAO28A Micros 200 RAS257C Decontamination amp rinse e Concerns Instrument decontamination before maintenance operation in the following cases Instrument removed from a biohazardous area Maintenance intervention on contaminated assemblies and instrument rinse Required tools Hexagonal keys Clamps Flat screw driver Torx keys Required products Fungicidal bactericidal virus killing detergent spray non corrosive for metals non plastic altering Bleach solution 12 C Deionized water Absorbant paper Distilled water ntervention time 1h30min Frequency On request Specific kit or consumables None Disposal gloves eyes protection and lab coat must be A worn by the operator Local or national regulations must be applied in all the operations RAS257C RAS257 2 1 Preparation 20min Switch on the instrument Run a Startup cycle then a Clean cycle Switch off the instrument and remove the supplying cable Open the instrument cover Spray the bactericidal cleaner on all assemblies that may provide biologic risks and wait fo
118. rinter power is Off when a result should be printed out or when the Reprint key is pressed The tube holder was not positioned correctly when the tube holder door was closed The tube holder was not positioned correctly when the tube holder door was closed Blank value is higher than specified values WBC 0 3x102 uL RBC 0 02x10 pL HGB 0 3 g dL HCT 0 1 PLT 10x102 uL 1 Sample holder does not open 2 Sample holder opens closes to fast or to low Startup unexecuted 1 Connect the printer cable 2 Restart sequence after 30 seconds or press the Start Pause key to reset error Select printer appropriately in F36 print format selection 1 Connect the printer cable then restart sequence after 30 seconds or press the Start Pause key to reset error 2 Turn the printer power On then restart sequence after 30 seconds or press the Start Pause key to reset error 1 Press the Startup Pause key then press the Service key several times to open the tube holder door 2 Reposition the tube holder so that it locks into position and close the tube holder door 1 Press the Startup Pause key then press the Service key several times to open the tube holder door 2 Reposition the tube holder so that it locks into position and close the tube holder door Check reagent level Check ground Perform a Backflush cycle F11 Perform a Bleach cleaning cycle F16 Check bubbling Close sample holder
119. rked on the ABX CRP REA package Press the Enter key again Only the PLT display will start blinking The instrument is now ready to enter the CRP reagent sensitivity factor B Using the Up or Down arrow keys enter the CRP reagent sensitivity factor B RAS259 6 ABX Micros 200 Technical Manual RAAO28A Micros 200 Press the Enter key again Only the CRP display will start blinking The instrument is now ready to enter the CRP reagent sensitivity factor C Using the Up or Down arrow keys enter the CRP reagent sensitivity factor C The entry of the CRP reagent sensitivity factors can be canceled if the Start Pause key is pressed EZ before the Enter key In this case the CRP reagent correction values remain unchanged Start this procedure over again from the beginning Check that the new ABX CRP REA kit is placed in the reagent cooling unit Close the sampling holder without placing any sample in the tube holder Calibration is performed When calibration is completed the instrument becomes ready for measurement The entered CRP reagent sensitivity factors are simultaneously printed out Check that the printed results agree with the CRP reagent sensitivity factors of CRP Calibration is required each time the reagent lot changes Run the ABX CRP TROL Low Medium and High controls and verify that they are within their assay limits noted on their packages When the CRP display blinks the Start Paus
120. rt F 57 function and repeat until you get a correct adjustment The needle must not touch the bottom of the CRP chamber Those values are CRP Needle and CRP Carriage values on configuration ticket Put the black WBC amp RBC cover back Run a diluent priming F 13 function Enter F 91 function Photometer adjustment The instrument automatically fills the flow cell with diluent and shows the CRP blank value on WBC display During this cycle the value displayed on WBC window will slowly decrease Perform F 91 five times in order to check the repetability of values and verify that the value does not fall more than 10 within 30 seconds By means of VR1 on CRP board adjust displayed value to 3800 10 A The adjustable range of VR1 is 270 degrees Attempting to turn it in excess will damage VR1 ABX Micros 200 Technical Manual RAAO28A RAS445 5 RAS445A RAS445 6 ABX Micros 200 Technical Manual RAA028A Exploded views CONTENTS I LIS a 9 2 2 board VIEW unas ii 9 6 3 R agent sensor 2 VIEW 9 7 A SA BE 9 8 5 CRP SY ut AU s 9 9 6 Sampling needle rinsing block view 9 10 7 Carriage sensor amp pulley view 9 11 DANO VISW Gde 9 12 9 RBC chamber 9 13 10 9 14 11 Power supp
121. s Minimum specimen volume CBC Mode CBC 10 QC Mode CBC 10yl CBC CRP Mode CRP 18pl QC mode CRP 18pL 4 Dilution ratios WBC 1 260 RBC PLT 1 15000 CRP 1 50 HGB measurement HGB chamber LED 555 nm Modified Drabkin method cyanmethemoglobin Light source Electroluminescent diode Wavelength 550nm 10nm CRP Measurement CRP Chamber LED 850 nm Latex Immunoturibidity rate method Light source Electroluminescent diode Wavelength 850nm 10nm Counting Aperture Diameters WBC 80 RBC PLT 50um Reagent Consumption ml Cvel Phe duration MiniDil LMG Lyse mi Cleaner ml R2 R3 ibis min s ml y m ml ml STARTUP 1 blank cycle SHUTDOWN 1 00 E 17 E 3 Analysis CBC 115 15 9 0 5 1 3 Analysis CBC CRP 430 30 3 0 5 0 9 0 1 0 1 0 2 Prime ALL Reagents 225 40 0 12 0 6 0 Prime DILUENT 1 24 27 0 Prime LYSE 131 11 6 Prime CLEANER 1 137 x 6 3 Complete Rinse 228 a CBC Bleach clean 2 56 2 E 5 Bleach clean 114 z E AUTOCLEAN 133 31 3 18 9 Hgb Photometer Adjust 055 6 0 1 3 Blank Adjust 055 6 0 S BACKFLUSH 0724 15 6 E Wake up from sleep mode 0 40 14 3 0 5 1 3 STARTUP cycle estimated duration and consumptions are given for one blank cycle control It could be a maximum of three cycles if Startup does not pass the first time
122. s WBC 1 00 1 21 RBC 0 77 1 00 HGB 0 90 1 15 HCT 0 90 1 20 PLT 0 98 1 50 MPV 1 09 1 31 CRP 0 88 1 09 ABX Micros CRP 200 Technical Manual RAA028A RAS259 3 RAS259C If the above range is exceeded the instrument may be malfunctioning Stop the calibration press Clean key and perform measurement again Using the up and down keys set the new WBC calibration coefficient obtained in previous step Press Enter key The RBC calibration coefficient is displayed in RBC display Similarly enter the new RBC calibration coefficient and press Enter key Perform as described previously for HGB PLT and MPV See Fig 1 Coefficients page 4 ABX MiCPOS CRPeoo WBC EJ WBC Calibration coefficient lt EB eco 8 e READY HCT e e RBC Calibration coefficient HGB Calibration coefficient HCT Calibration coefficient PLT Calibration coefficient BUSY PLT ERROR RDW Calibration coefficient CYCLE READY BUSY ERROR CRP Calibration coefficient Fig 1 Coefficients After the coefficient MPV the RDW coefficient is displayed Do not change RDW coefficient it is A factory adjusted In case of 18p the PDW coefficient is displayed Do not change PDW coefficient it is factory adjusted Press Enter key until you exit function The ready LED lights up to indicate that the instrument is ready for measurement Measure the control blood to confirm that th
123. stic procedures Methods and technologies WBC RBC and PLT Impedance HGB Spectrophotometry Cyanmethemoglobin method CRP Latex immuno turbidity rate method Calculations from stored data that was directly measured for HCT MCV MCH MCHC RDW MPV PCT and PDW In the USA PCT and PDW parameters are for research use only and not for use in diagnostic procedures Sample quantity CBC mode 10uL CBC CRP mode 18uL QC CBC mode 10 QC CRP mode 18 Throughput analyses CBC mode Within 75 seconds 48 samples hour CBC mode Within 270 seconds 14 samples hour QC CBC mode Within 75 seconds 48 samples hour QC CRP mode Within 270 seconds 14 samples hour Reagents ABX Minidil LMG 10 Liters ABX Cleaner 0 5 L ABX Lyse 0 4L ABX CRP REA CRP R1 CRP R2 CRP R3 total 50 mL Physical Specifications Power Requirements Power supply from 100 V AC to 240 V AC 10 50 Hz to 60 Hz Power consumption Maximum 150 VA Printer Depends on printer model See printer s manual Operating Temperature and Humidity 18 C 30 64 F 86 F room temperature Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly to 50 relative humidity at 40 C 104 F Dimensions and Weight Dimensions 31 x 41 x 40 cm 11 8 x 16 7 x 15 7 in Weight 18 Kg 40 lbs ABX Micros 200 Technical Manual RAA028A Specifications Physical Specification
124. t on the rear of the instrument Connect the 3 pin plug of the power cable into the oulet of 100 240V AC with 50 or 60 Hz Place the instrument where an independent outlet can be used Use an outlet different from the one used by a device which easily generates noise such as centrifuge 4 When placed on a metal table ground it as well ABX Micros 200 Technical Manual RAAO28A RAS256 7 RAS256C 3 6 Setting the language Make sure the instrument is OFF then configure the jumper E1 on the Main board E1 jumper configuration language setting 3 000 Japan cas United States no display with Hemalink sas English OO u Japan 3 7 Priming the reagent at installation EY if the power is supplied when the reagent 15 not primed in the tube the condition at unpacking error code E 6 E 15 etc refer to section 7 may occur frequently Be sure to prime the reagent at installation Before turning ON the power be sure to check that the reagent and waste liquid tank are correctly installed and tubing is connected Press PUSH on the top of the instrument and open the upper door While pressing the Service key set the power switch to ON Error code E 19 will be displayed This code indicates startup in the service mode When F1 is displayed on the sample display unit release the Service key By releasing the Service key the F1 display turns to N 1 and the input Ready LED will light up P
125. then pull it on the piston See Fig 10 O ring installation page 8 ABX Micros CRP 200 Technical Manual RAAO28A RAS258 7 RAS258C Fig 10 O ring installation Put piston in syringe body See Fig 11 Piston installation page 8 Fig 11 Piston installation Place the bottom plate to close syringe and tighten the 3 Torx screws Install back the syringe on the frame and connect CRP syringe tube back Continue maintenance before you put back the front panel EM 5 Liquid syringe O rings and Diluent piston replacement Liquid syringe is located behind front panel to access this part you must remove the front panel See procedure RAS 268 Front panel dismantling of this manual 1x F0020373000 1x FAA036A 2x FAA055A 1xH1008304002 Push the piston assy in the upper position and clamp the diluent and lyse tubing See Fig 12 Liquid syringe page 9 RAS258 8 ABX Micros 200 Technical Manual RAAO28A Micros 200 Fig 12 Liquid syringe Disconnect diluent lyse and sampling tubings from the 3 syringes and the tube on the liquid syringe side Unscrew the 4 fixation screws and remove the liquid syringe See Fig 13 Liquid syringe screws page 9 065021673 Fig 13 Liquid syringe screws Unscrew the 4 CHC screws A and the 2 FHC screws B in order to remove the syringe bottom plate See Fig 14 Bottom plate page 10 ABX Micros 200 Technic
126. top plate and the old O ring ABX Micros 200 Technical Manual RAAO28A Micros 200 Spread a little amount of silicone grease between two fingers and apply a very thin film of grease on the new O ring Install the new O ring and the top plate on the vacuum syringe Install back the syringe body on the frame using the 4 screws Push back the piston inside the syringe body before tightening the 4 O ring fixation screws CHC M2 5x8 Tighten those 4 screws with a dynamometric screw driver to 400mN m Fig 2 Syringe screws Connect back the tube the vacuum syringe 2 Rinsing block O ring replacement 1x FAA053A Push backward the sampling needle carriage and lift up the sampling needle Remove the clip maintaining the needle in its upper part See Fig 3 Clip page 3 Fig 3 Clip Unscrew the 2 screws of the rinsing block See Fig 4 Rinsing block screws page 4 ABX Micros 200 Technical Manual RAAO28A RAS258 3 RAS258C Fig 4 Rinsing block screws Remove the needle from the rinsing block then the O ring See Fig 5 Needle O ring page 4 Fig 5 Needle O ring Spread a little amount of silicone grease between two fingers and apply a very thin film of grease on the new O ring Reinstall the rinsing block in reverse order Use the A301 dynamometric screw driver to tighten the rinsing block screws to 100mN m RAS258 4 ABX Micros
127. tube Microtainer 1 0 1 F29 By pressing the Enter key the CRP syringe is syringe moved down to the lowest position and the Busy LED starts blinking Press the Enter Key again the CRP syringe is moved up to the highest position home F3 Setup Menu Select a number within a display window by using the Up or Down arrow keys and then press the Enter key to accept the change and move to the next display window and or exit the menu LRL NN RR CC F31 Date Format Select a setting 0 MMDDYY format 1 DDMMYY 2 YYMMDD 3 YYDDMM F32 Date Time adjust Set year month YY MM DD HR MIN day hour and minute F33 Cleaning The number of 0 Never frequency analysis requiredfor 1 1 100 next autoclean can be set up in range of 1 to 100 F34 Units Select a unit O Japan STD Juss 2 Standard 3 Inter 2 F35 Serial Output Select a format of 0 Trans Off RS232 RS 232C output 1 Internal ABX 1 2 Standard 3 Internal ABX 2 F36 Printer Select a printer Second Copy Print Limits Printers Print parameters 1 format 0 No 0 0 Histo The printer must be 1 Yes 1 Yes 1 Epson 0 turned ON 2 Seiko 1 Yes 3 Standard F37 Print setup Printout of the instrument configuration F38 Printer Select the printout Print LMG results parameter 2 of WBC 3 Diff data 0 No Set 1 1 Yes 6 4 ABX Micros CRP 200 Technical Manual 28 Software versions Software overview
128. um E A Gu 1 gt lt lt Te 5 Sim 0695 99 01 or 4 Rs e DO AS 98 21515 Hs Lu gt 2AS ETA gt gt ona 82 1 lt MET SiL 8 i gt va lt lt 2 399 5291 858 5291 3Hd EINI gt AIVENS a Piei 6 I QING anm vites 2 IAS IYS gt Te 5 551068840 EN gt NO LONIH z lt S gt bid 13H Ls 10928899 2 TOW z 8 gt ona Emo 0 gt lt 850 529 3Hd ZINI VIVES NIN E a me rar gt gt Gre CHO 25 5 2H ve 25 18 6592 sons Elio gt gt Te i35 qe C i es 2 2 50460 025996980 b aHa VI TA 80192621093 lt gt ORD ger va cian 30 11819 SINO gt 271 405 One sz 80 181 Yd CNLWENS qu Vel SHIS as di 19743 ST dm vites 951068840 93 813 e LINO 21IN0 A1 in lt ina 9006200013 9149 ORE E l ES onm v ep rs TT 2 quo 931 AS MEE 1VEO lt 0699 969 201 4 T 35 2 5 gt FQ 2 UUL 033 gt 5807 ZN Od 82 N p3 13H eno gt gt DN N39 VZ gt AMM a cr C 038 81 2 ev ean 958 954 501 she Md m E SS 1068840 b4 13H 8r gt an a b 039 _ gt EE o SEDEN MEAE 35 ee h 930 10 08591 20 amp ANA 949 5 n 991968840 COD 2 Li Nas lt 0699
129. unit and the reagent cover open close is correctly placed and is not damaged ABX Micros 200 Technical Manual RAAO28A RAS256 3 RAS256C 3 2 Installing the reagents ABX Minidil LMG Connect the connector end of the attached ABX Minidil LMG tube to the Diluent connector on the tube connecting block on the back of the instrument at the lower left Attach the other end of the Diluent tube to the head of the attached Diluent 10L straw Pull out the cap of the ABX Minidil LMG tank open the cap attach the Diluent straw and close cap of the straw Open the remaining quantity check window at the lower side of the front of ABX Minidil LMG packing case f 4 5 27 lighten the screw of the connector firmly Place the ABX Minidil LMG box at the same height as the main body 4 Do not bend the DILUENT tube Do not have it cross a power line to avoid the effects of noise The length of the tube should be no longer than 1 5 m 59 inch and as short as possible Do not apply force to the joint of the tube connecting block 4 Prevent dirt from getting into the reagent reagent and tube should not be exposed to direct sunlight 4 ABX Lyse may contains cyanide Handle it carefully ABX Cleaner and ABX Lyse Open the reagent door 1 A Lyse straw A and a Cleaner straw B are already installed See Fig 1 Reagent straws page 4 Fig 1 Reagent straws Remove those straws Connect the
130. urement Ready LED Lights up when measurement is possible or operation commands can be input with the operation keys Busy LED Lighting of the LED indicates that the instrument is not prepared for measurement When this LED is lit input with the operation key cannot be done When ending operation for the day shutdown this LED lights up or blinks This LED lights up during the sleep state Error LED Lights up or blinks if any error occured in the instrument Sample number display Displays sample numbers If an error occurs the error code number is displayed During the measurement of the blood count item CRP item counts down and displays the measurement time left Measurement result display Displays the result of each measurement item When setting the time and during calibration displays numerical value of each operation ABX Micros 200 Technical Manual RAA028A 2 5 ABX Micros CRP 200 3 3 Operation panel The User function keypad on the ABX Micros CRP 200 has 8 basic keys with their functions indicated either above or below the key Those keys allow the operator to initiate cycles access menu functions reprint or transmit results modify specific menus and enter calibration coefficients The primary function of each key is descibed in detail PRIME CLEAN REPRINT CAL SERVICE SU 1 L Y A ex ENTER Prime key Reagent ABX Minidil LMG ABX Lyse and ABX Cleaner will be
131. ustment See 10 RBC PLT latex adjustment page 10 RAS262 Bubbling See RAS264 Bubbling adjustment Temperature T adjustment See RAS265 Thermic adjustment Software versions Software overview Locate the tip of the needle at the same level as the bottom of rinsing block then run F61 The pulse count from this position to the needle home position is displayed on RBC display Adjust the position of the needle home sensor until the values becomes 70 5 The current channel is displayed on HGB display By means of R97 on Main board adjust the HGB channel according to the room temperature using the HGB blank adjustment value chart Check the voltage between TP19 and TP31 Press Enter key to measure the aperture voltage It must be between 58 5 and 62 8V Run the WBC latex adjustment LYM Value blinking in WBC display shows the LYM target depending of latex lot used usually 57 1 Press the Enter key Value blinking in RBC display shows the GRA target depending of latex lot used usually 180 2 When values are stable adjust gain by mean of R74 on Main board Run the RBC PLT latex adjustment RBC Value blinking in WBC display shows the RBC target depending of latex lot used usually 744 1 Press the Enter key Value blinking in RBC display shows the PLT target depending of latex lot used usually 644 2 When values are stable adjust RBC gain by mean of R81 on Mai

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