M220 Manual
Contents
1. N A 11 30 12 2 35 admin specialist specialist Specialist 11 30 12 4 50 N A 11 30 12 2 35 admin Add New User Edit User Remove User Add New User Edit User Remove User s Figure 5 6 User Administration Screen M220 User Manual Page 28 Covaris The New User Information window will appear See Figure 5 7 e Enter a user name password and User Role type Click Ok e Click Save in the User Administration dialog GE New User Information User Name Password User Role Operator Figure 5 7 New User Dialog 6 3 5 Remove a User e Select the user that needs to be removed e Click Remove User s button from the User Administration Form See Figure 5 6 e Click Save in the User Administration dialog 6 3 6 Edit a User e Select the user that needs to be edited Click Edit User s button Administrator can edit user password and role Click OK Click Save in the User Administration dialog Q Edit User Password specialist1 User Role Specialist hi Figure 5 8 Edit User Dialog M220 User Manual Page 29 Covaris Administrator user can create methods and lock these methods Subordinate users will not have the ability to modify method e Create or edit a method See Section e Select the Locked check box See Figure 5 8 e Click Save Create New Method cans Basic Settings Temperature i gt J Method Name Protection ih Locke2. Step Type Deisy Piopeines Detay Time seconds Figure 4 4 Add a Delay Step 5 1 3 Choosing a Prompt step displays a specified message on the screen and pauses execution of the method until the operator clicks OK or Cancel See Figure 4 5 nae CT Add Method Step otto Step Type Frompt Properties Message Text OK Come Figure 4 5 Add a Prompt Step 5 1 4 By choosing a Repeat step the user can choose how many iterations they would like to apply to a method See Figure 4 6 Figure 4 6 Add a Repeat Step 5 1 5 When all method parameters and steps have been defined click on ok to save changes M220 User Manual Page 21 Covaris 5 1 6 From the Basic Settings Tab once a method has been created general operation buttons are available for the following functions Create New Method a Method Name Protection ga Locked Untitled Creation Notes Setpoint C Temperature Range C to Required Run Time 00h 01m 00s Add Step Insert Step Edit Step Remove Remove all Duplicate Group Steps Move Up Move Down Select All Treatment 60 sec ya m save _save as _cancet_ E Figure 4 7 Available Functions p Add Step Allows the user to add a step to the end of the method Insert Step Allows the user to select a step and insert a new step either before or after the selected step Edit Step Allows the user to edit the select
3. The power and temperature charts lower right corner of the Run Screen will display average power and temperature data during the running process The progress bar will display the percentage of the run that has been completed The user can at any time while running a method click Pause to pause a running process When the method is paused the user may continue the process by clicking on Resume button or terminate the process by clicking on the Abort button When the method is complete a dialog box will be posted over the Run Screen notifying the user If necessary click the mouse in the text entry area to set the focus then notes may be added either via the keyboard or an alternative input device For instance a barcode reader may be used to capture data directly from pre labeled samples When finished simply open the Safety Cover and remove the sample tube 3 6 Instrument Light Light The instrument light is located above the acoustic assembly and inside of the Safety Cover By default the light will be lit once the instrument is turned on This light may be turned off by clicking the Light control on the Run Screen 3 7 Draining water and cleaning ON Special care needs to be taken when draining the water from water bath Remove the tube holder and place it on a clean dry surface Draw the water from the acoustic assembly using the provided syringe It is important to use the plastic tip on the syringe
4. 25 sec Duty Factor Cycles Burst 20 0 200 Treatment Peak Power 50 0 Treatment Peak Power 50 0 O 32s 20 0 lt Treatment 505 Duty Factor Cycl 20 0 Peak Power 50 0 X Connected to Instrument M220 C Run History Setup Maintenance About Required Run Time 00h 01m 47s Min C Setpoint C Max C 18 0 20 0 22 0 oyato Tostrument Status d Log In nstrument Status Status System Check Override Light ON Water Temperature Water Level Door Override E System Check Light Water Temperature Water Level Door Figure 3 3 Instrument Status Panel and Instrument Light Control To achieve an acceptable status on these conditions e The M220 system automatically adjusts the water temperature to match the set point and Water Temperature status will turn green when temperature is within the Min and Max limits typically 2 degrees above and below the set point The default set point in the absence of a selected method is 20 C e Filling the acoustic assembly with the Tube Holder in place to the top of the tube holder will allow the Water Level indicator to turn green See Section 3 3 e The Door Closed status will turn green as soon as the Safety Cover is completely closed The temperature condition may be ignored by left clicking the Override box
5. Check the USB cable Restart the instrument and SonoLab Go to Setup and reconnect manually Terminate other Windows applications Reboot the computer Restart the system Clean the water level measure channel Covaris Appendix A Suggested M Series IQ OQ PQ All Covaris instruments are rigorously tested at the Manufacturing Facility prior to shipment This is done to ensure that your Covaris device is of the highest quality and meets your needs for consistent efficient and reliable Sample Preparation In the event that you require a method for independent verification of your Covaris Instrument a set of recommended IQ OQ PQ activities and requirements are provided in the following Checklist format If desired this Checklist can be completed and retained for your personal records These suggested activities are basic in nature and intended only for high level instrument qualification Given your situation you may wish to supplement these activities with application specific performance activities and acceptance criteria DEFINITIONS IQ Installation Qualification ensure correct instrument has been delivered and installed in accordance with manufacturer s requirements OQ Operation Qualification ensure instrument is functioning in accordance with specification and all measurement sensors safety interlocks and software operate as intended PQ Performance Qualification demonstrate the instrument continues t
6. Figure 5 11 Page 31 Covaris 6 4 Maintenance Screen By selecting the Maintenance tab or category See Figure 5 12 allows an operator to view e Instrument Information e RF Power Board Calibration e Frequency Sweeping Parameters e Transducer Parameters e Update instrument firmware Instrument Model Transducer Instrument Serial Number Firmware Update Controfler Board Serial Humber TE Connected to Instrument M220 COM Figure 5 12 The Maintenance Tab Only service or factory personnel are able to modify the system configuration An administrator is allowed to perform a Firmware Update but must log in to do that See Section 6 3 2 6 4 1 Firmware Updates Covaris Instruments contain computer controllers which are responsible for operating the instrument and communicating with SonoLab On occasion it is necessary for Covaris to make changes to the firmware that operates the instrument When SonoLab connects to an instrument it checks the version of firmware that is running on that instrument If the instrument s firmware is out of date SonoLab will alert the operator and suggest that a firmware update should be performed See Appendix C Update Sonolab Software pra The Instrument Firmware is out of date Go to the Maintenance Tab and Update Firmware Figure 5 13 Firmware Out of Date Alert M220 User Manual Page 32 Covaris The About Tab provides user basic info
7. M220 User Manual Page 4 Covaris TABLE OF CONTENTS 1 0 INTRODUC TION ica ch cece ct teen ew areca E veetodv gee suubedeneucbact 7 1 1 Overview of the Manual ssiri ree deecs nested race ae derek peu evci mec lore ia 7 1 2 The M220 and the CovarisS Process csccccsssssssseeeeeeeeecceensnseeeeeeeeeeesnsneeeeeeeecoesneeeeeessenonanees 7 20 INSTALLATION AND SETUP caer ice eet vate cee prea weer edged aed ew erences nt vee 7 2 1 PS SO TIMOUY Se ceie ce seeetanaey cee se pemee cele econ ce eewuceunenet saneeueeerne denen ieenn seecueeneshaueeudee cue cedsnceoseeeceensauucuncnenecenes 7 2 2 Installing the Tube Holder naannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn nnmnnn 9 2 3 SOMOL AD MS tall AU ON sisca inoia a E aaa Saaai 10 2 4 Sla NG SONO LA Da E E T 11 3 0 OPERATION ciecia E E E E E E sae rebieesucncesesuuredeecone 12 3 1 Gone ar OVOTVIGW senon E E 12 3 2 Preparing the M220 for US acuis i a Rai 13 3 3 Filling the Water Bath eerenepeRrnete ecm oenr nner reece ments tt et ene entree me ri tien tren testi ert ere 7 crn 15 3 4 POAC ING TENG SAN onre ewe este cece paced os ecsteea cece nlesie ees Secure eee esceauieedesseuuneteecensecestees 16 3 5 Ru NNINO A CT OG sien cccicsdc os calc a a a 17 3 6 BUDS CE CUTI TAG LORI oe ste caee Geeta cseviesecccteeascesunnedsaveccusnecunesecsctseneseccncut E stent 17 3 7 Draining water and cleaning secessinwecsecsscnccenteenice cuss secnctecestessacscsesten
8. Hew foal ery i ZIL M220 DHA Shenma Foaia his DHA Shasmeg Prion imi T setup 5 The Setup Wizard will start Follow the installation wizard step by step until the installation is finished 71220 ONA Shearing Protect T Welcome to the M220 DNA Shearing Protocol Setup C Wizard The erstellen wall guide you through tho shops ragun to mrstell M220 DRA Shoeeng Proteool ani ysr comput WARNS Thies sane W Rea Fe pri sent bey Oo pynig hew eed inter Daa ae aide lata Gr dieinibyticn ia pregnani or ei oe IEM BHH in Seed giil ge criminal panties andwil be B prone scaled te a Mima mie sible pu the lw Cancel Heri M220 User Manual Page 49 Covaris The installer places the protocols in the directory C Users Public Public Documents Covaris SonoLab Methods a GO i Libraries Documents Covaris Sonolab Methods M220 DNA Sheanng e LSS E SACA M220 DNA Shoring P Fate Edit View Toots Help Organize Share with gt Burn New folder i Documents library sider DNA_0150_bp_050_ul_Screw_Cap_microTUBE pro DNA_0150_bp_130_ul Snap Cap _microTUBE pro DNA_0200_bp_050_ul_Screw_Cap_microTUSE pro DNA_0200_bp_130 ul Snap Cap _microTUBE pro DNA_0300_bp_050_ul Screw Cap microTUBE pro DNA_0300_bp_130 uf Snap Cap microTUBE pro ONA 0400 bp 050 ul Screw_Cap mecroTUBE pro DNA_6400_bp_ 130 ul Snap Cap _microTUBE pro DNA_0500_bp 050 _ul Screw _Cap_microTUBE pro D
9. The area required for the instrument see attached drawings is approximately 12 wide by 17 deep by 10 high 80cm x 48cm x 25cm Allow sufficient space in front of the bench to access each component of the installation DO NOT cover the air intake at the front penal Power Requirements Provide at least two standard outlets one for the laptop computer and one for the M220 The M220 draws a maximum of 500 VA The Covaris M series instruments are configured with a universal power supply WARNING To prevent the possibility of electrical shock always plug the system into a grounded power source Water Requirements The M220 system water bath requires about 15 milliliters of AFA grade Water which should be changed daily Water is added to the system by manually filling the bath with the water bottle supplied with the system Covaris recommends AFA grade Water PN 520101 It is also possible to use highly purified water Highly Purified Water at least ASTM Type III or ISO grade 3 Moving or Packing the Instrument for Transport When moving the instrument within a laboratory or between nearby laboratories please employ the following guidelines e Turn the instrument off Disconnect line cords and USB cable e Remove the laptop from the top of the instrument it is held in place by Velcro strips e Remove the sample holder and lift and remove the safety cover e Remove remaining water from the acoustic assembly e Lift the uni
10. generated from transducer For example if Peak Incident Power is set to 10 Watts and Duty Factor is set to 15 the Average Incident Power will be approximately 1 5 Watts However the real average incident power from the transducer may vary due to factors such as sample tube type water level and water temperature Cycles per Burst This parameter indicates the number of electrical signals used in each acoustic burst of the transducer One may define Cycles per Burst from 50 to 1000 in the M220 for different applications Advanced users may use this parameter to fine tune their experiments Duration This parameter defines the amount of time that the sample is under a treatment The measurement of the Duration is in seconds The maximum time for any treatment is 3600 seconds Should a longer treatment be necessary the Duplicate or Group Steps can be used to extend the treatment At the top of the screen the software displays the Average Incident Power calculated simply from the PIP and Duty Factor In the M220 instrument the maximum Average Incident Power is 20 Watts for example if the 50 PIP 50 Watts and 40 Duty Factor are selected M220 User Manual Page 20 Covaris 5 1 2 Choosing a Delay step introduces a fixed time delay into a method User can set the sample to settle between two different treatment steps The delay is specified in seconds See Figure 4 4 a OY Add Method ep m
11. indicated by the M220 at the lower left of the Run Screen Note The Run tab is NOT visible if the instrument is disconnected Go to the Setup tab click on Communications and observe the Connection State If the indicator is red and the state is Disconnected click Connect button Once the connection status changes to Connected the system is ready for use Click on the Run tab to return to the Run Screen NOTE Covaris recommends that the Laptop computer be employed exclusively for instrument control and that operation of additional application software be minimized M220 User Manual Page 11 3 0 Instrument Light Covaris OPERATION 3 1 General Overview The principal elements of the M220 are depicted below in Figure 3 1 Tube Holder Insert lifted out Acoustic Assembly Electronics Cabinet Computer Location a Safety Cover Chiller Air Intake Chiller Sensor Module Chiller Fan Exhaust Acoustic Transducer Figure 3 1 Principal components of the M220 instrument The Safety Cover is used to protect users from any potential acoustic hazard during the treatment The acoustic treatment will not be started if the Safety Cover is open The Acoustic Assembly is used to transmit the acoustic energy from transducer to the sample through the coupling media AFA grade water The assembly incorporates an Acoustic Transducer which converts electrical signals into ultrasonic acoustic
12. 1394 Bus host controllers b Z Imaging devices gt lt gt Keyboards p F Mice and other pointing devices b Monitors b amp Network adapters a F Po p DB Proce p Sound video and game controllers gt lll System devices 4 Univers i g Generic USB Hub g Intel R ICH8 Family USB Universal Host Controller 2830 f Intel R ICH8 Family USB Universal Host Controller 2831 uw ft Intel R ICHA Family USR Universal Host Controller 283 M220 User Manual Page 48 Covaris Appendix E Running the SonoLab Protocol Updater The SonoLab Protocol Updater is used to update the M220 DNA Shearing Protocols preinstalled on the computer running SonoLab 7 Latest version of the SonoLab Protocol Updater can be found in the resource section of Covaris website htto covarisinc com resources registered users login m series You will need to create an account to access this area of the website NOTE The Updater may overwrite existing protocols Please examine Release Notes prior to installation to verify what additions and changes will be made 1 Close SonoLab before installing the Updater 2 Download the latest version of the SonoLab Protocol Updater on Covaris website 3 Open the M220 DNA Shearing Protocol Installer zip file 4 Double click on the Setup application gt s Archi 2 210031 M220 OMA Shewin p Bl Search 2 Pile Edit Yaw Took Help Crgienne Open a
13. If Override is checked then Water Temperature is considered acceptable regardless of actual value The Status icon will continue to show a green check or red X but with a small horizontal line through it to indicate the Override condition Once all conditions are acceptable and a method has been loaded the Run button will illuminate and the selected acoustic Method can be started If user wants to modify an existing method one click the Edit button in the Method window after the method is highlighted The method editor window will show up The Edit function allows user to modify an existing method M220 User Manual Page 14 Covaris To transmit acoustic energy to the sample vessel the Acoustic Assembly must be filled with AFA grade water Covaris recommends AFA grade Water PN 520101 You can also use highly purified water at least ASTM Type Ill or ISO grade 3 To fill the water bath follow the steps listed below Cover moved up for water filling Cover moved down to retard contamination AFA grade Water Covaris PN 520101 Figure 3 4 Water bottle and tip cover As shown in Figure 3 4 fill the wash bottle with AFA grade water Slide the blue cover up to fill the water bath slide the cover down after filling to keep the nozzle clean Wash bottle tip Water Sense Aperture Fill until water is visible here Figure 3 5 Fill through the Water Sense Aperture using wash bottle As
14. Method Name Protection gp Locked ntitled Creation Notes Method Name Required Run Time 00h 01m 00s Setpoint 20 0 5 Temperature Range 18 0 to 22 0 Add Step Insert Step Edit Step Remove Remove All Duplicate Group Steps Move Up Move Down Select All ma i o u u u u uuu uo O PTE Peak Power Duty Factor Cycles Burst Ava Power m he Group Repeat soo so 200 75 oxy Save Figure 4 8 Group or Repeat Method 5 1 8 Type a Method Name and click Save to save this method for future use 5 2 To edit an existing method pull down the name of an existing method see Figure 4 1 and click Edit 5 2 1 Methods may be Edited in a similar manner as creating a method if the user has the required Authorization see User Authentication M220 User Manual Page 23 Covaris 5 3 From the Basic Settings Tab 5 3 1 Setpoint is the target value that the instrument will automatically aim to reach User can choose a Temperature Setpoint from 6 0 C to 40 0 C 5 3 2 Temperature Range This function will set the upper and lower temperature limit to 2 degrees of the setpoint Edit Existing Method Basic Settings Temperature lt Temp tab Method Name Protection Test Method 1 Creation Notes TemperatureSetpoint Required Run Time 00h 04m 09s Setpoint C 20 0 a Temperature Range C 18 0 to 22 0 Add Step Inser
15. ed story Grows og Fies Polder C Users Pubbc Documents Coveris Senotab Log irse C Automancaby delete History Fes older than 120 18 days 2 Automancaty delete Log Fees cider than 120 18 days L Save Changes Restore Oedauits Discard Changes KE Connected to Instrument M220 COMI Figure 5 9 File Settings M220 User Manual Page 30 6 3 9 Creating Folders Covaris User can create folders for their methods within SonoLab 7 for a better organization of files Methods can be saved as new or existing folders Create or edit a method See Section 5 0 Click Save As Click New Folder and enter Folder Name 4 Save Method As Folders Last Run af Methods Argenis test 2 1 11 30 2012 1 58 57 PM Argenis test 2 o 11 12 2012 12 24 47 PM Argenis Test 11 12 11 12 2012 11 47 04 AM Bruce s first method 4 30 2012 11 29 26 AM Test Method 1 New Method Folder FolderName SY 11 30 2012 1 19 31 PM x Method File Name Save Cancel Figure 5 10 Click on newly created folder or an existing folder to save method Click Save Folders Files New Folder Method Name Last Run P M220 DNA Shearing P November 12th Methods Novemember 2012 G29 Treatment 11 P 30 Method File Name Test Method 12 1 12 M220 User Manual
16. instrument is unpacked please locate the M220 on a flat surface near two power outlets Use both sides of the base Figure 2 1 to lift the instrument Do not use the acoustic assembly to lift the instrument If necessary adjust the four adjustable feet to ensure the instrument is stable and level Do not remove the adjustable feet They are needed to ensure proper air flow from the system chiller M220 User Manual Page 8 Covaris 1 Peel off the backing from the Velcro strips on the Computer Loading bars Place the computer on top of the glue side of the strips as shown in Figure 2 2 Gently press the computer to secure it in place HEHEHE EERIE Center the Taan computer on top of the M220 as shown Figure 2 2 Secure the computer on top of the M220 as shown USB port To computer Power Switch Ventilation fan Power Inlet Figure 2 3 The back panel of the M220 instrument 2 As shown in Figure 2 3 plug the AC power cord into the power inlet located at the back panel of the instrument Plug the AC power cord into the power outlet 3 Plug the AC adaptor into the computer and into the power outlet 4 Connect the USB cable into the USB port located at the back panel of the instrument Figure 2 3 and USB port on the notebook computer 5 Place the Safety Cover on the hinges please refer to Figure 2 1 6 Place the Tube Holder in the Acoustic Assembly housing refer to section 2 2 below The entire
17. instrument may be relocated in this assembled configuration by disconnecting power lifting from the sides relocating and repeating the appropriate assembly steps 2 2 Installing the Tube Holder M220 User Manual Page 9 Covaris Open the Safety Cover and place the Tube Holder into the Acoustic Assembly Note that the undercut at the forward left corner of the tube holder should be aligned with the pin on the assembly Undercut gt ss np Tube Holder gt a e 4 Pin Figure 2 4 Place the Tube Holder in the Acoustic Assembly 2 3 SonoLab Installation SonoLab 7 is an application used to control a Covaris AFA Adaptive Focused Acoustics instrument for processing a sample It is designed to create edit and run a sample processing program called a method It has provisions to run pause or stop a method and view execution histories which contain a record of key instrument parameter values during a run It also contains editors for customizing system settings and performing maintenance functions SonoLab 7 has been pre installed in the computer purchased with the M220 instrument No further installation or configuration is required if the computer is purchased with M220 instrument Users can directly go to section 2 4 to start the SonoLab program If it is necessary to install or re install SonoLab 7 or instrument drivers on a computer please contact Covaris for the latest versions do
18. operate with a water bath and includes a solid state heater chiller apparatus to control sample temperature NEVER run a method without water in the acoustic assembly this could damage the transducer The instrument is equipped with a water level sensor to protect the transducer The system will not allow the acoustic wave treatment to start unless an adequate volume of water is detected AFA grade water should be used to fill the water bath Empty the water bath and wipe it dry EVERY day with a lint free cloth DO NOT leave water in the water bath for an extended time as there is no water filtration or water cleaning system with the apparatus Do not employ isopropyl alcohol ammonia based or abrasive cleaners on the acoustic assembly as these will damage the acrylic or transducer surfaces The Tube Holder should be stored in a dry place or in the M220 Accessories Storage Station when not in use Establish a standard of operation and periodically test equipment as described in Appendix A of this manual DO NOT load third party hardware software or parts onto the system without consulting with Covaris M220 User Manual Page 3 Covaris When used in accordance with written instruction and under normal operating conditions the Covaris instruments are guaranteed to be free of defects in MATERIAL and WORKMANSHIP for one 1 year from the date of original delivery by an authorized representative Any component which proves defective durin
19. signals The assembly also incorporates a Sensor Module with water level and temperature sensors to ensure that these are appropriate during acoustic treatments The Tube Holder is designed to hold the sample tube at the appropriate position in the acoustic field during processing It is normally inserted into the top of the Acoustic Assembly The Computer provides an interface and software to the operator for controlling the M220 instrument through SonoLab 7 The Electronics Cabinet provides the hardware for controlling the acoustic transducer and the temperature during sample processing The Front Light is normally lit when the instrument is turned on The user can disable the light from the main control panel of SonoLab The Chiller is used to control the water bath temperature It is able to chill or heat the acoustic assembly to maintain the water bath at or near the set point programmed in acoustic Methods No external chiller is required WARNING During treatments objects immersed in the focal zone above the transducer will be exposed to high acoustic intensity when the system is activated Always close the Safety Cover prior to starting a method Do not tamper with the door closed sensor system M220 User Manual Page 12 Covaris 3 2 Preparing the M220 for Use To start an acoustic treatment four conditions must be met There must be an acoustic method selected in the Method box Water Temperature must be within ac
20. the Setup tab and choose the User Authentication subtab 9 Uncheck the Enable Authentication check box 10 SonoLab 7 x and the instrument are now ready to use Install Windows Instrument Driver Windows Drivers are software files required to support communications between a computer and any USB Universal Serial Bus device Specific drivers are needed to support communications with the M220 A Ifa laptop is configured for internet access either via wireless link or Ethernet cable and automatic driver updates then the first time the M220 is connected to a laptop Windows will automatically install the necessary driver and provide updates as these drivers are installed Please note that drivers for several devices within the M220 will be installed sequentially These include a Generic USB Hub Covaris RF Controller Board Covaris RS 485 Port Covaris Serial Port 2 and Covaris Serial Port 3 Please allow all of these drivers to install and for Windows to confirm successful installation of all drivers before starting SonoLab 7 B Ifthe laptop has internet access but is not configured for automatic updates then either change this setting to automatic or instruct Windows to search for each driver automatically as this query appears M220 User Manual Page 44 Covaris C Ifthe laptop does not have internet access please follow these steps e M220 User Manual Connect a 220 Series instrument to the i
21. to prevent the scratch of the transducer surface Water needs to be drawn as completely as possible Use the disposable cleaning swab to sweep the water channels in the Acoustic Assembly Wipe the surfaces of the transducer and the metal housing with lint free cloth and air dry for next use Store the Tube Holder in a dry place or in the M220 Accessories Storage Station PN 500352 close the Safety Cover 3 8 Shutdown Procedure lf the instrument will be idle for an extended time e g overnight or a weekend shut down the instrument using the following procedure Empty the water bath store the Tube Holder in a dry place CAUTION Leaving water in the acoustic assembly for an extended time may allow algae growth which will interfere with sample processing Exit SonoLab software by clicking on the red X in the upper right corner If there are unsaved changes in Setup Screen a message box will be popped up asking if user wants to save the changes Select Yes to save the changes and leave SonoLab or select No to leave SonoLab without saving the changes The user can also select Cancel to continue working on the SonoLab To shut down the laptop the user should select Shut down from the Start menu on the Windows screen to power down the computer The instrument should be powered off after the SonoLab software has closed To turn off the instrument the user needs to move the power switch to OFF position Th
22. to run a time course at a level of power optimized for efficient chromatin fragmentation when used in conjunction with Covaris TruChlP kits Naming convention is as follow ChIP _ duty factor time M220 User Manual Page 18 _ Covaris 5 0 CREATE AND EDIT METHODS SonoLab supports the creation of new methods and editing of existing methods from the Run Tab A method consists of one or more treatments delays and message prompts 5 1 To create a new method click the New button in the Method window see Figure 4 1 Run Edit History Setup Maintenance Help Abi Method Team SSCS Trew J eat Pull down list Notes w Required Run Time 00h 00m 25s Min C Setpoint C Max Treatment 25 sec Peak Power Duty Factor Cycles Burst 50 0 20 0 200 Edit an existing method Create a new method Figure 4 1 Controls for creating and editing a method The Method Editor window will appear See Figure 4 2 From the Basic Settings tab Select the Add Step to create a method Basic Te SS 58 Setti n g S ic Settings Temperature Protection Q Locked Method Name Untitled Creation Notes Setpoint C Temperature Range C to Save s Cancel Figure 4 2 Method Editor Window The Add Method Step window appears as shown in Figure 4 3 The Step Types available in this screen a
23. 000 3 3 1995 for EMC EU EMC Directive 2004 108 EC Also FCC Part 15 Class A radio emissions requirements for the USA and ICES 003 Class A for Industry Canada AFA grade Water Covaris pn 520101 or Highly Purified Water at least ASTM Type Ill or Nee neces a Programmable 6 0 C to 40 0 C Soft limits 4 0 C and 52 0 C Hard limits 2 0 C and 42 0 C Notebook computer supplied by Covaris Microsoft Windows 7 Application Software Covaris SonoLab 7 Data Input Keyboard mouse Chiller Solid state chiller for heating and cooling built in Power 0 48 Watts Intertek M220 User Manual Page 34 7 0 TROUBLESHOOTING System Trouble Analysis Chart Covaris SYMPTOM POSSIBLE CAUSES SOLUTION Door status or water level status is not green Instrument unable to communicate with hardware Disconnected mode Software Application is unresponsive Low water level is detected Objects may be blocking the when water bath is correctly water level sensor filled M220 User Manual 2 Cover is not closed completely Water level is too low Tube holder is misaligned causing low water level Cable is loose or damaged SonoLab is having difficulty identifying the instrument via USB Windows resources are being employed by another application Page 35 Re close the front cover Fill water bath to the fill line Verify that the holder is fully seated into the acoustic housing
24. Covaris For research use only M Series USER MANUAL instrument developed for high performance sample preparation using adaptive focused acoustics Product Name M Series Model Numbers M220 Software SonoLab 7 Covaris UNIVERSAL PRECAUTIONS Universal Precautions should be followed on all specimen samples regardless of whether a sample is known to contain an infectious agent Laboratories handling specimen samples are advised to comply with applicable parts of the following governmental and clinical standards or their equivalent in the country of use Centers for Disease Control CDC Universal Precautions for Prevention of Transmission of HIV and Other Bloodborne Infections published 1987 updated 1996 Clinical and Laboratory Standards Institute CLSI GP17 A2 Clinical Laboratory Safety Approved Guideline Second Edition published 2004 ISBN 1 56238 530 5 Clinical and Laboratory Standards Institute CLSI M29 A3 Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guideline Third Edition published 2005 ISBN 1 56238 5674 Occupational Safety and Health Administration OSHA 29 CFR 1910 1030 Bloodborne Pathogens International Standards Organization ISO 15190 2003 Medical Laboratories Requirements for Safety Trademarks Patented or patent pending and registered or registration pending are trademarks of Covaris Registered names and trademarks used in this document even when not
25. NA_0500_bp_130_ul Snap_Cap_microTUBE pro DNA_0800 bo _050_ul Screw Cap macroTUBE pro DNA_0800_bp_130_ul Snap Cap microTUBE pro DNA_1000 bp 130 ul Snap Cap microTUBE pro DNA_1500 Dp_130_ul Snap _Cap_microTUBE pro DNA_2000 bp 200 ul Clear miniTUBE pro DNA_3000_bDp_200 ul Blue minTUBE pro DNA_5000_bp 200 ul Red maniTUBE pro If the instrument does not perform as expected please contact Covaris Technical Support Technical Support On going assistance with the operation or application of the equipment and or troubleshooting is provided via e Telephone 1 781 932 3959 during the hours of 9 00am to 4 00pm Monday through Friday o United States Eastern Standard Time EST o Greenwich Mean Time GMT 05 00 e E mail queries to techsupport covarisinc com M220 User Manual Page 50
26. T LB Processors amp Sound video and gar JE System devices Update Driver Software Disable Uninstall Scan for hardware changes Properties Universal Serial Bus controllers Launches the Update Driver Software Wizard for the selected device Page 45 Covaris f When the driver software update dialog opens select the option to Browse my computer for driver software Update Driver Software RF Controller Board How do you want to search for driver software gt Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device unless you ve disabled this feature in your device installation settings gt Browse my computer for driver software Locate and install driver software manually g Point the file open dialog to c Program Files Covaris Driver to find the necessary instrument drivers K L Update Driver Software RF Controller Board Browse for driver software on your computer Search for driver software in this location gram Files FTDI CDM 2 08 02 WHQL Certified Include subfolders gt Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device h Click next You will be notified that Windows can t verify the publisher of this drive
27. amage to the transducer and electronic circuits could result if the transducer is operated without water Operation of the system without water in the water bath voids the warranty CONTACT COVARIS INC SHOULD YOU HAVE ANY QUESTIONS CONCERNING THE EQUIPMENT Warranty Services The purchased equipment is covered by a twelve 12 month warranty which includes all the service and support necessary so that the customer can operate the equipment successfully Extended warranties are available at the end of the original 12 month warranty period Services included with the original purchase of the system are Technical Support On going assistance with the operation or application of the equipment and or troubleshooting is provided via e Telephone 1 781 932 3959 during the hours of 9 00am to 5 00pm Monday through Friday o United States Eastern Standard Time EST o Greenwich Mean Time GMT 05 00 e E mail queries to techsupport covarisinc com or applicationsupport covarisinc com M220 Instrument Repair Repair of all parts including the laptop from normal operation of equipment are provided in our Service Depot All labor charges are included Instrument packaging and ground shipping will be provided Failure due to accident abuse or improper operation is not covered An Operator s Manual is provided with the equipment This manual includes sections on the operating instructions maintenance guidelines and troubleshooting tips
28. and the Computer and Application Software which provides users an interface to control the device The Covaris Process uses adaptive focused acoustic energy to precisely control cavitation and acoustic streaming within the sample treatment vessel in a non contact isothermal fashion Focused acoustic energy is generated and delivered to individual samples The mechanical energy imparted on the sample results in a controlled series of compression and rarefaction events INSTALLATION AND SETUP 2 1 Assembly The following items should be found in the package of the Covaris M220 instrument o Quick Start User Guide o User Manual o Notebook Computer with AC Adapter 50031 1 o Cables 1 USB cable 1M220 power cord 1 Computer AC Adaptor power cord Non US customer only o Accessories Fill Drain Kit 500299 M220 User Manual Page 7 Covaris e 1 100ml wash bottle e 120ml Syringe e 2 Re sealable plastic syringe tips Cleaning Swab Kit 500298 e 10 disposable swabs AFA grade water 125ml o M220 Safety Cover o M220 Main Unit CAUTION Lift the M220 out of the box by its baseplate Do not lift by the Acoustic Assembly Lifting the unit by its acoustic assembly may damage the instrument omputer Loading Bars Tube holder D Computer Station M220 Main Unit Hinges Safety Cover Lift Here Acoustic Assembly Chiller Exhaust On both sides Figure 2 1 A front view of the M220 instrument After the
29. bsite htto covarisinc com resources registered users login m series You will need to create an account to access this area of the website Install SonoLab Application 1 Download the latest SonoLab version and save it on your desktop 2 Open the SonoLab 7 x zip file 3 Right click on the setup exe application Run as administrator 4 You must Agree to the Program License Agreement click next to put SonoLab 7 x in C Program Files and then click Next to begin the installation 5 Click Yes to enable the installer to install SonoLab 7 x if Windows User Access Controls have not been disabled 6 Turn on the instrument and plug the USB cable into a USB port on the computer 7 Navigate to C Program Files Covaris SonoLab on the computer 8 Right Click on Sonolab exe and select Run As Administrator SonoLab will open its splash screen Depending of your previous SonoLab version you may have to update your instrument Firmware 1 A dialog box will pop up to alert you that the firmware of the instrument is out of date Click OK 2 Click on the Setup tab and select the User Authentication sub tab 3 Check the check box labeled Enable Authentication 4 Click on the login window 5 Use the username admin and the password Sonolab to log in 6 Click on Maintenance Tab and click on the Firmware Update Tab 7 Click on the Start Updating Firmware button 8 When the firmware update is completed go back to
30. ceptable Min and Max limits Water Level must be at or above that required for correct operation The Safety Cover must be closed The Run button will remain gray and inactive until all four of the conditions are met To select and load an acoustic method click the pull down menu of the Method combo box as shown in Figure 3 2 and a list of existing method names along with their detailed information will be shown Navigate through the list with the vertical scroll bar and select a method Please note that several acoustic methods will come pre loaded into your system These pre loaded methods cannot be edited Run History Setup Maintenance Abot Method Method combo box Notes a Pull down menu Run Notes Edit an existing method Create a new Required Run Time 00h 01m 47s method Min C Setpoint C Max C Run Creation Notes mM Peak Power Duty Factor Cycles Burst Treatment 50 sec 5 m Peak Power Duty Factor Cycles Burst B m m m m mm o Connected to Instrument M220 COM7 Figure 3 2 Selecting and loading an acoustic method M220 User Manual Page 13 Covaris The next three conditions are displayed in the Instrument Status panel see Figure 3 3 of the Run Screen An acceptable condition is indicated by a green check mark in the Status box Ifa condition is not acceptable the status will show X Method Test Method 1 Notes w
31. d Loc ked Fest metroa N Creation Notes Required Run Time 00h 00m 01s Setpoint C 20 015 Temperature Range C Cs S to 25 018 fasa Step Insert Step Jil Edit Step Remove Remove All Jif Duplicate Group Steps Move Up Move Down Jif Select an Pesk Power Outy Factor Cycles Burst Avg Power Treatment 1 sec x one CE L E N T A E R E E 4 gt save f saveas cancet Figure 5 8 Locked Method 6 3 8 File Settings By selecting the File Settings tab or category See Figure 5 9 user can change file locations and set up a scheduled task to automatically delete the old log files By default log files older than 120 days are automatically deleted For change to take effect user must close out of Sonolab and reopen To change File Setting e Click the Browse button e Select or create a new folder for new location and click OK e Click Save Changes To change Auto Deletion of Files e Click on Automatically delete check box to turn on auto delete e Enter or move the up down arrow to specify how old the log files must be to be deleted e Click Save Changes Run History Setup Maintenance About User ainme Log Out Categories Communications User Autherbratos V file Settings Coererutec aons Method File lt Folder ber Authen c ation lc Users Pubic Documents Cowaris Sonolsb Methods Browse Fie Seng P ory Pes Polder Users Pubbc Documents Covens Sanot
32. d tube in to the device sample holder Create or Load a New Method in SonoLab with the following settings and Save As Method Test or other naming convention yes e Peak Incident Power 50 0 Duty Factor 10 NO e e Cycles Burst 50 e Duration 60 seconds for a total of 1 minutes M220 User Manual Page 38 Covaris M SERIES OPERATIONAL QUALIFICATION OQ 2 of 2 DATE INSTRUMENT S N COMPLETED BY LOCATION Click mim Run or on the SonoLab Main Screen to start the recently created Method Verify reasonable Power Levels are displayed by SonoLab For the Method Test power display is 3 7 Watts Before the Treatment ends Click m Stop and verify the acoustic method stops Back in the SonoLab main screen click Run once again to start the treatment Before the Treatment ends click Pause and verify the Method is suspended Click ESEN Resume and verify the Method continues where it left off Verify a Run Results Dialog Box is displayed after 1 minute and indicates The operation for method Method Test completed successfully COMMENTS M220 User Manual Page 39 Covaris M SERIES PERFORMANCE QUALIFICIATION PQ 1 of 2 DATE INSTRUMENT S N COMPLETED BY LOCATION Action Requirements Ref Section Requirement Met 20W Power Test Program a New Method in SonoLab with the following settings and Save with appropriate t
33. e 5 3 Te mr AY M220 we SonoLab Run History En omen About d Categories As z W E y Communkatwas User Autherncanen Y Ple Settings aon COM Pert Port Type Avatable Ports Conmecton State Communication sis ik LO Disconnect Connect Disconnect Comected Simdan Simulation Mod Save Changes Restore Orfautts If Discard Changes Figure 5 3 The Setup Tab A user can select Simulation mode See Figure 5 3 to run SonoLab 7 without connecting to an instrument In the Run tab user can create and edit methods Click on the Simulate button to run a simulation of the method Ki Connected to intrument M220 COM 7 Cowan So cLabs bet aes set SonoLlab 7 2 Simulation C frun History Setup Maintenance About Cyt Method S DHA _0200_bop_130_u_Snap_Cap_mkroTUSE ei Pause New Ed Notes R Time h 02me4 5 equired fun Time 00h 02m 405 in nent m l min C Mag C Statws System Check Override Degas Pump l so 250 J Degas a Y Water Temperature o Paused J Treatment 160 sec J Water Level Seac Power Duty Factor Cycles Murs d Water Tank ON oF 20 s Jf Zalety Cover EEE j t maw P 2 wst Simulation Mode Figure 5 4 Simulation Mode M220 User Manual Page 26 Covaris 6 3 2 User Authentication By selecting the User Authenticati
34. e power switch of the M220 is located at the back of the instrument next to the power cord of the instrument M220 User Manual Page 17 Covaris SonoLab has protocols preinstalled on the computer running SonoLab 7 These methods have been developed by Covaris scientists and are ready to use for most common applications To select a preloaded protocol click on the pull down button to make the list appear 4 0 USING A PREEXISTING METHOD Run Edit History Setup Maintenance Help Abi Method Testmethodi O Z o Z oo ooo O or Trew Required Run Time 00h 00m 25s Pull down list Min C Setpoint C Max C 25 sec Duty Factor 20 0 Peak Power 50 0 Cycles Burst 200 Covaris regularly updates these methods and add new ones The latest version of the SonoLab Protocol Updater can be found in the resource section of Covaris website htto covarisinc com resources registered users login m series You will need to create an account to access this area of the website 4 1 DNA shearing protocol DNA Shearing protocols are specific to the mean DNA fragments size that you re looking for as well as sample volume and microT UBE vessel used These protocols are stored in a folder called DNA Shearing for M220 Naming convention is as follow DNA size bp volume ul type of vessel 4 2 Chromatin shearing protocols Chromatin shearing protocols allow you
35. ed step Remove Allows the user to remove the selected step Remove All Allows the user to remove all the steps Before the remove a confirmation window will appear asking the user if the user wants to remove all the steps Duplicate Allows the user to duplicate a selected step Group Steps Allows the user to create a group step with the multiple selected step s A Create Step Group window will appear allowing you to specify how many iterations the user wants to repeat the steps Move Up Allows the user to move the selected step up one level If the selected step is already on the top of the list it has no effect on the method If the selected step is in a group a confirmation window will appear asking if the user wants to move the step out of the group Move Down Allows the user to move the selected step down one level If the selected step is already on the bottom of the list it has no effect on the method If the selected step is in a group a confirmation window will appear asking if the user wants to move the step out of the group Select All Allows the user to select all steps created M220 User Manual Page 22 Covaris 5 1 7 Whena method has been created the user can group a step to extend the treatment User can also repeat on treatment or a group of treatments A Group step is used to repeat 1 or more method steps a pre specified number of times Create New Meth Basic Settings Temperature
36. etcassslcsuaceassseteucecseeuveeussccasies 17 3 8 SIMUTO OWI PROG COUN G sica aa Eea a E a 17 AQ USING A PREEXISTING ME HOD e asassnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nennen nnn 18 4 1 DNA Sh arihg Protocol ci Ea EEE 18 4 2 Chromatin shearing protocols sssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn nnmnnn mnnn 18 50 CREATE AND EDIT METHODS veisins a eiaa 19 6 0 SonoLab APPLICATION SOFTWARE cccccsssssssseeeeeceeecnesseeeeeceecconssseeeeeeeecconnssseesseesoes 25 6 1 BRN sy OO acces seesaw saise E emmeoosecanscet sec eens couascesseccecscssueeds2sauuneuesacesamendesdatransedencte 25 6 2 FSO SCEE arse pete on ge cet wet ete cern ee etree deterrent 25 6 3 SEUD SI aonn wea ee a ee wei wes wea ce cleans cece ee tee cee eee cate 26 6 4 Maintenance SCT CIN acca neste eae eee cee seemed emanates 32 6 5 ADOUT Wa ae E ct ete E eee cdeiee ene ineees 33 70 TROUBLESHOOTING aga cil ec E EAA dome ecient oxen Ea EEA 35 Appendix A Suggested M Series IQ OQ PQ aaansssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nenne neneman 36 Appendix B M Series Site or Transport Preparation cccccssssseeessessseeeseeensseeseceessseeseeeenseesseoeensenssens 42 Appendix C Updating SonoLab Softwar e ccccssscseeeesessseeesecsssseeeeeoessseeeseoesseeeseooesseeeeeonnseeeseoensseessens 43 Appendix D Installing SonoLab on a new computer and installing Window
37. g the stated period will be repaired free of charge or replaced at the sole discretion of Covaris F O B Woburn Massachusetts U S A provided the defective component is returned properly packaged with all transportation charges prepaid The customer is expected to perform basic diagnostics and component replacement with telephone support from Covaris personnel If Covaris personnel are required to perform on site repair all travel related costs are paid by the customer A limited warranty as specified may apply to certain components of the equipment Warranty Warranty Exceptions This warranty is void if failure of the software or hardware has resulted from accidents abuse improper maintenance or repair or misapplication by the customer It is also void if damage is caused by any unauthorized attachments or if modifications are made to the equipment Removing or tampering with the Safety Enclosure will void the warranty and the customer will assume all liabilities This warranty is limited to the original purchaser and is not transferable The software will perform according to the accompanying written materials and the medium on which the software is delivered is free of defects in materials under normal use and service The warranty is void if damage has resulted from third party software not intended for use with the system The high power focused transducer is designed to give maximal mechanical energy output in water Permanent d
38. h ON OFF Switch and verify 1 Instrument Fans are now audible F YES F NO M220 User Manual Page 37 Covaris M SERIES OPERATIONAL QUALIFICATION OQ 1 of 2 DATE INSTRUMENT S N COMPLETED BY LOCATION Action Requirements Ref Section Requirement Met Section 1 Instrument Connection Verify computer is connected to instrument via USB cable provided Power on the computer and log in as required Note If Laptop is not provided by Covaris refer to SonoLab Driver installation sections of User Manual Double click the SonoLab shortcut on the computer desktop verify the YES application starts and SonoLab main screen successfully displays NO Verify the SonoLab software recognizes the instrument and indicates the YES instrument type at the top of the screen F NO Section 2 Temperature Measurement Verify Bath Temperature is displayed in SonoLab and is able to F YES equilibrate at 20 C Bath Temp Reading C M NO Section 3 Safety Interlocks Run Method Ensure a red X is present for Water Leve status with no water in the YES water bath Fill the water into the bath and verify the Water level Status changes to a green check mark I NO Open the Safety Cover Ensure the Door Status is denoted with a red YES X Close the Safety Cover and verify the status changes to a green heck mark JS NO Install an appropriate size
39. imi Mode DMA Shears WLI GS Pe SORDI ed A S400 EITAN iton Runtime Information y FAA SSA PH TI IHIH FPH PSL 2 1550 PH TENI ISEH PH HINH 15E PY HEMI Ler Les Pe GPG DOLE Lees A G26 D012 beer Ae Toone ta oc iio lt Method Run Details heft h PIHA eS FH Piit h AAJ Aa ids PM Pehla FH _ Be 12 be Pe Pit ER AALAN STA FH MANANA 1512H PM AS60129 Le Pe _ fn ATIDA 5S Pe HEITE 125130 Fe ee 2 Ls Pe Si M02 Tea Pa PSL 2012 H Fa BSG Larisa FE fun fetads Shep Betas ERL Mathds Trhitreni Clih T Connected bo Inwtment Miao COMT Corot iori fee il 7 Tronen LH La CEJ CH Average Power amp Temp Graph Figure 5 2 Example of History Tab CAUTION The history list can become long and slow the system By default log files older than 120 days are automatically deleted See Section 6 3 8 for details M220 User Manual Page 25 Covaris 6 3 Setup Screen The Setup tab Figure 5 3 allows an operator to configure the communication port used to control the instrument enable disable user authentication to configure user log in accounts specify folders used to save methods history files and log files 6 3 1 Communication By selecting the Communication tab or category user can select COM Port or USB Port to communicate with the instrument By clicking on the connect disconnect button user can manually connect or disconnect from the instrument See Figur
40. itle i e 50W Test e Peak Incident Power 50 0 e Duty Factor 40 e Cycles Burst 50 e Duration 300 seconds for a total of 5 minutes Set chiller temperature at 20 C Obtain a tube holder Covaris 500296 and a Screw Cap microTUBE Covaris 520096 YES Insert the holder into the water bath housing of M220 and fully fill the tube with water and place into the holder NO Specify the above treatment parameters Denote the Water Bath Temperature and verify it is between 18 22 C WATER BATH TEMP a Start the method by clicking Run button Monitor the power reading and record value below Verify that turbulence is seen in the water around the tube Allow method to run for at least 1 minute and record the average power level displayed by SonoLab SonoLab Avg Power Watts Verify the average SonoLab Reported Power is between 15 22 Watts Note If the Expected Power value is not within specification repeat test on a different tube and ensure tube does not contain cracks pits or other damage If SonoLab power is still not within specification contact Covaris Customer Service for support M220 User Manual Page 40 Covaris M SERIES PERFORMANCE QUALIFICIATION PQ 2 of 2 DATE INSTRUMENT S N COMPLETED BY LOCATION User Defined Test As Required COMMENTS M220 User Manual Page 41 Covaris Appendix B M Series Site or Transport Preparation Placement of Equipment
41. lick next to put SonoLab 7 x in C Program Files and then click Next to begin the installation 5 Click Yes to enable the installer to install SonoLab 7 x if Windows User Access Controls have not been disabled 6 Turn on the instrument and plug the USB cable into a USB port on the computer 7 Navigate to C Program Files Covaris SonoLab on the computer 8 Right Click on Sonolab exe and select Run As Administrator SonoLab will open its splash screen Depending of your previous SonoLab version you may have to update your instrument Firmware 1 A dialog box will pop up to alert you that the firmware of the instrument is out of date Click OK 2 Click on the Setup tab and select the User Authentication sub tab 3 Check the check box labeled Enable Authentication 4 Click on the login window 5 Use the username admin and the password SonoLab to log in 6 Click on Maintenance Tab and click on the Firmware Update Tab 7 Click on the Start Updating Firmware button 8 When the firmware update is completed go back to the Setup tab and choose the User Authentication subtab 9 Uncheck the Enable Authentication check box 10 SonoLab 7 x and the instrument are now ready to use M220 User Manual Page 43 Covaris Appendix D Installing SonoLab on a new computer and installing Windows Instrument Drivers Latest version SonoLab can be found in the resource section of Covaris we
42. nstrument control computer using a USB Cable Plug the instrument in and power it up Click on the Start menu Control Panel Hardware and Sound Under Devices and Printer click on Device Manager The Device manager window will appear File e 9 m H m amp 4h Covaris PC D p Batteries b 9g Computer Disk drives p gt iy Display adapters gt DVD CD ROM drives gt c IDE ATA ATAPI controllers p gt IEEE 1394 Bus host controllers gt lt gt Keyboards E Monitors gt Y Network adapters Other devices Da RF Controller Board Pa RF Controller Board gt XJ Ports COM amp LPT D J Processors a Sound video and game controllers gt gii System devices gt Universal Serial Bus controllers p A Mice and other pointing devices _ fg RF Controller Board port 3 amp 4 j RF Controller Board port 3 amp 4 Under Other devices right click first device RF Controller Board and select Update Driver Software File Action View Help 4e m O B me ees 4 By Covaris PC 2 Batteries M Computer ci Disk drives E Display adapters lt i DVD CD ROM drives Ca IDE ATA ATAPI controllers IEEE 1394 Bus host controllers lt gt Keyboards F Mice and other pointing devices E Monitors Network adapters lp Other devices Ja RF Controller Board Up RF Controller Boa Da RF Controller Boa Qe RF Controller Boa P Ports COM amp LP
43. o meet specification appropriate to routine use M220 User Manual Page 36 Covaris M SERIES INSTALLATION QUALIFICATION IQ DATE INSTRUMENT S N COMPLETED BY LOCATION Action Requirements Ref Section Requirement Met Section 1 Shipping Contents and Instrument Condition Instrument is successfully unpacked and all items noted in Packing List are present YES Denote any missing items here F NO There is no damage evident to the instrument or accessories If damage is present r Description of Damage YES Corrective Action F NO Section 2 Verify Proper Site Conditions Adequate space and ventilation are present for the instruments and the room conditions temp humidity etc are within recommended limits for the device per the User Manual YES If unacceptable Non Conformity I NO Corrective Action Two 2 appropriate power outlets are available for the device and the YES laptop ia T NO Section 3 Component Assembly Connect the laptop and instrument to line power The line cords are F YES verified to be in proper condition and match the Wall Outlet provided Connect laptop to instrument via USB Cable NO Unpack the front cover and tube holder Verify there are no visible cracks or damages Follow the installation manual to assemble the F YES device Fill the water bath with AFA grade Water Verify there are no leaks F NO Power ON instrument wit
44. oenrotier 383C USS Componte Devce USD Composite Device y USE Root Hub USS Root Hub Launches the Update Driver Software Wizard for the selected device k Repeat steps E to I three more time for all devices under Other devices until they are all gone A total of 5 times Note When you click on Browse my computer from the Update Driver Software window the path for the FTDI drivers will remain No need to re enter l Goto C Program Files Covaris Sonolab m Right Click the Sonolab application n Click on Run as Administrator o Verify connection Status is green and connected p Verify instrument information on the about Sonolab tab q Close out of SonoLAB 7 r Double click on the SonoLAB Series vX XX on the desktop to verify application works Close out M220 User Manual Page 47 Covaris This completes the device driver installation At the end of the installation the following devices should be added to Device Manager under Ports COM amp LPT Covaris RF Controller COMS3 Covaris RF Controller COM4 Covaris RF Controller Port 3 amp 4 COM5 Covaris RF Controller Port 3 amp 4 COM6 In addition 4 devices should be added to Device Manager under Universal Serial Bus Controllers Covaris RF Controller Board Covaris RS 485 Port Covaris Serial Port 2 Covaris Serial Port 3 File Action View Help m H mle G5 Human Interface Devices p gj IDE ATA ATAPI controllers p IEEE
45. on tab or category See Figure 5 5 user can establish 3 levels of users and identify the user by means of a log in out format Run History Setup Maintenance About S Login Categories Communications User Authentication File Settings User Communications User Authentication Authentication Fie settings Enable Authentication C Enable Authentication lt User Administration U se r Administration Save Changes Restore Defaults Discard Changes X Connected to Instrument M220 COM7 Figure 5 5 The User Authentication Tab By default authentication is disabled In order to enable user authentication a log in id and password is required Please contact the Covaris Customer Service to obtain Administrator user name and password The SonoLab authentication model supports 3 types of users e Operator e Specialist e Administrator The Operator is allowed to run a method from existing methods Operator can review the history screens and system configuration information Operator cannot edit an existing method or modify the systems operating mode A Specialist performs all of the duties of an operator In addition a Specialist is allowed to edit existing methods unless methods have been locked by their creator The Administrator performs all operations of a Specialist In addition has the responsibility of being able to configure the system s operating mode An Administrator can e Create and Remo
46. r software Click on Install the driver software anyway Dont install this driver sowane FO te Cee ee ue ee TCT eS Se eee fa teu aE nstall this driver software anyway ny mall drier softens phaned from your manae 1 e os dic Urge cotterwe em other bE many haere poe e oe e rdonmmahon wo See gril M220 User Manual Page 46 Covaris Windows will install the driver and post the following dialog box Ww L Update Driver Software Covaris RF Controller Board Windows has successfully updated your driver software Windows has finished installing the driver software for this device Covaris RF Controller Board j Under Other devices right click device USB Serial Port and select Update Driver Software Z Device Manager fos File Action View Heip ds miU a ras IEEE 1394 Bus host controllers gt Keyboards A Mice and other parting devices aX Mondors G Network adapters oy Other devices Gy FF Controller Board Je BF Controller Boardiport 3 amp 4 i RF Controfier Board port 3 amp 4 ap USB Senel Pot O Ports COM ALE Update Denver Software 0 Processor Oratie amp Sound vdeo ari Uninstall D System devices a Uniwersal Serial Scan for hardware changes Covers PEO Generic USS Generic USE Hat Generic USE Hud Intel 5 Series 2400 Series Chipset Family USB Enhanced Host Controller IDH v Intel R S Series 2000 Series Chipset Family USS Enhanced How C
47. re Treatment Delay Prompt and Repeat 5 1 1 Choosing Treatment allows editing of the four acoustic parameters M220 User Manual Page 19 Covaris Add Method Step Step Type gt Step Type Treatment Value Value 6 3 Average Incident Power Watt 0 10 20 30 40 50 60 70 80 90 100 Peak Incident Power Watt 0 50 100 150 200 250 300 350 400 450 500 Duty Factor percent Cycles Burst count 50 100 200 300 400 500 600 700 800 300 1000 Duration seconds g z 1 300 600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600 OK Cancel Figure 4 3 Add a Treatment Treatment parameters can be chosen either by sliding a control left or right by clicking on up down arrows in the value box or by directly editing the Value box The treatment parameters are Peak Incident Power PIP This parameter measures the peak electrical power used to drive the ultrasound transducer In the M220 instrument the adjustable range of PIP is 2 5 to 75 Watts Duty Factor This parameter defines the ratio between the on time of the transducer and total operation time and is expressed as a percentage For a given Peak Incident Power a larger Duty Factor will results in more acoustic energy generated from the transducer Users can define the Duty Factor from 0 1 to 50 in the M220 instrument The product of the PIP and Duty Factor gives the approximate Average Incident Power
48. rmation about the instrument such as serial number software version board serial numbers etc See Figure 5 14 M220 User admin f Log Out Version 7 2 0 64 Unit Type Serial Number 2080 Controller Board Serial Number co00084 Controller Board Version 5868 Power Board Serial Number P05301 Power Board Version 5868 Transducer Serial Number Transducer Center Frequency kHz Transducer Impedance 14 Gill Street Unit H Woburn Massachusetts 01801 USA Phone 781 932 3959 FAX 781 932 8705 http www covarisinc com View Help Service Information X Connected to Instrument M220 COM7 Figure 5 14 About Tab M220 User Manual Page 33 Covaris SYSTEM SPECIFICATIONS Bench top high intensity acoustic transducer temperature monitoring Treatment System and controlling device water bath with sample holder and safety enclosure 75 Watts Peak Incident Power 12 W x 17 D x 10 z goer x 43cm x 25cm Ambient Temp Range 15 to 30 C Regulatory Labeling CE ETL Mark for Product Safety WEEE Complies with Low Voltage Directive 2006 95 EC Certified to IEC EN ANSI UL 61010 1 2004 and CAN CSA C22 2 No 61010 1 2004 Safety 2nd Edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements Complies with Class A Industrial Scientific Medical ISM equipment under EN 61326 1 2005 EN 61000 3 2 2004 and EN 61
49. s Instrument Drivers 44 Appendix E Running the SonoLab Protocol Updater ccceecccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeenseeeeeeeeeoes 45 M220 User Manual Page 5 Covaris Intentionally blank M220 User Manual Page 6 1 0 2 0 Covaris INTRODUCTION 1 1 Overview of the Manual This manual contains installation operation and service instructions for the Covaris M220 Focused ultrasonicator It contains information essential to the proper use and care of this equipment Should any unforeseen problems occur with the normal operation of the equipment contact Covaris Technical Support immediately The following definitions apply in this manual There is a PDF version of this manual available on the computer desktop Open the manual by double clicking the user manual icon A hypertext version of this manual can also be found in the Help tab of the SonoLab Software 1 2 The M220 and the Covaris Process The M220 Focused ultrasonicator is based on Covaris Adaptive Focused Acoustics AFA technology This instrument is designed to provide researchers an integrated stand alone tool to be used in laboratory environment The instrument is ideal for those who work with DNA shearing and Sample preparation The system is comprised of the Treatment System which delivers the acoustic energy to the sample the Safety System which protects users from inadvertently contacting the high intensity acoustic energy
50. shown in Figure 3 5 place the tip of the wash bottle in the water sense aperture and fill the water bath by squeezing the wash boitle until the water reaches and fills the central aperture of the tube holder Approximately 15 ml of water will be required M220 User Manual Page 15 Covaris 3 4 Loading the Sample Loading Position Sliding weight Sample tube Figure 3 6 Sliding weight in loading position before loading a tube As shown in Figure 3 6 lift the sliding weight step 1 and place it at the loading position by turning the sliding weight 90 step 2 either clockwise or counter clockwise to secure the sliding weight Place the tube into the aperture at the center of the Tube Holder and make sure the tube sits all the way down in the holder Running Position Figure 3 7 Sliding Weight sits on the top of the sample tube and stays at running position As shown in Figure 3 7 place the Sliding Weight on the top of the sample tube by rotating the step 1 and lowering step 2 Sliding Weight The Sliding Weight will be maintained at running position Close the Safety Cover to ensure that the Door status in SonoLab turns to a green check M220 User Manual Page 16 Covaris 3 5 Running a Method Open the Safety Cover load the sample tube to be treated into the center of the Tube Holder and close the cover When the Run button is green the selected method may be started Click Run to start
51. specifically marked as such are not to be considered unprotected by law Information subject to change without notice For Research Use Only Not for use in diagnostic procedures Publication P N 010157 Revision F October 2014 Product covered by Patent No US6 719 449 US6 948 843 and other applications Copyright 2014 Covaris all rights reserved Covaris Inc 14 Gill St Unit H Woburn Massachusetts 01801 1721 USA Printed in U S A M220 User Manual Page 2 Covaris Warnings For safety of operating personnel Make sure that the equipment is properly grounded DO NOT operate if it is not properly grounded The unit is equipped with a power plug appropriate for the destination country DO NOT under any circumstances remove the grounding prong from the power cord DO NOT attempt to operate the equipment with the safety cover in the OPEN position or without water in the acoustic assembly the acoustic system will not work If there is any indication that the Safety System is not functioning properly DO NOT operate the equipment and contact Covaris immediately If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To prevent damage to the equipment The instruments are designed to operate in ambient laboratory conditions e g 15 to 30 C 60 to 86 F DO NOT operate the instrument in a cold room environment the system is designed to
52. t Step Edit step Removel Remove All Duplicate Group Steps Move Up Move Down Select All W Peak Power so 200 200 100 5 4 Temperature Tab 5 4 1 User to set an auto resume for the minimum and maximum temperature set point Example If customer sets a minimum temperature value of 18 degrees and the instrument temperature drops to 17 degrees The unit will pause If auto resume value is set to 18 5 once temperature rises to this value the unit will automatically run 5 4 2 User has the option to choose whether pause or abort method when Maximum or minimum Temperature error occurs Basic Settings Temperature Current Limit Auto Resume Value C Value C Maximum Temperature f o2s Minimum Temperature Auto resume Temperature Setpoint 20 0 Auto resume when water temperature is within Auto Resume values Maximum Temperature Error Action Pouse mated o Pause Abort Minimum Temperature Error Action Abort method Method M220 User Manual Page 24 Covaris SonoLab APPLICATION SOFTWARE The main components of SonoLab software include 6 0 Run compose or select methods to run History view system usage history Setup system setup by user Maintenance instrument parameters set by factory or service personnel About information on system configuration 6 1 Run Screen The Run tab Figure 5 1 is
53. t by its base plate not by the acoustic assembly Hand carry or transport by cart When packing the unit for shipment please follow the guidelines above Additionally if feasible obtain a packing box from Covaris and employ the foam end cap inserts to support the instrument lf this is not feasible wrap the main instrument in ample bubble wrap or similar packing Wrap and package accessories laptop safety cover sample holder cords separately to avoid damage Please contact Covaris Customer Support for guidance and assistance M220 User Manual Page 42 Covaris Appendix C Updating SonoLab Software Latest version of SonoLab can be found in the resource section of Covaris website htto covarisinc com resources registered users login m series You will need to create an account to access this area of the website Un Install Existing SonoLab 1 Turn off M220 series device and close SonoLab software application 2 Navigate to the Control Panel gt Programs gt Uninstall a program 3 Select the program named SonoLab and click uninstall 4 Are you sure you want to uninstall SonoLab Click Yes 5 The desktop shortcut titled SonoLab v7 will remove itself Install updated SonoLab Application 1 Download the latest SonoLab version and save it on your desktop 2 Open the SonoLab 7 x zip file 3 Right click on the setup exe application Run as administrator 4 You must Agree to the Program License Agreement c
54. the principal user screen for the system See Section 3 5 for details on operating the instrument through the Run Screen Pause oes oe ha ri Run Tine C MORO ri Satus Min SE gaporp E Has SE Suha yten Check dwerice Light LaLa mi Ha Water Fenperatues wf Miter Liral Teramat D H te Pe maen 28 oe F a u Path Pe mat ala Fora e Erime ON a T Taim 2 Y tee aa Pink homm ds Pater Erhm 5 a3 W araw a Tamant m 0 nee Poet Pome Sete PADa Pr i T P atl E Omeia fe ermet H20 COM Figure 5 1 The Run Screen 6 2 The History tab Figure 5 2 shows the history of every method performed on the instrument It displays the selected method s runtime information and run details A graph of the actual average power and temperature during each treatment step is stored History Screen Lat Fen On Lit Date On fated By Crashed Oe Credted iy Method Fun Detads BADIA Jiti A ORAS bee a AO Pe Bert Tira l lt LAS TET A aAA iiag Ae ae se rd Teme WAI Lee al GLA 1111525 AA AIAH Lea AM BABI Lisi Pe cal crt eta Ce SIAD IT AB PASH Leo a G26 D2 bP Pe oat ru CHA OAM bo 11 u Snap Cap miooTUEE Moai DHA Shear SPL 154 FPN YADI EGAN SAOL 3599 AH facbo Erue E OME pgo ba IIi ol inap tas mero Til MIZI DA Sheera WLI Pe SHDN Geto aM Sarthe 1390 aM faeta TUTE One 0000 bo 00 ul Renae Che mera Then Ja A pki LIS Pe Sao ee a Satie REIT aM ia OOM Port Dha 090 ba Omi ul Sram Cag mirat
55. ve User accounts e Secure methods e Setup File Settings and automate the deletion of old log and history files e Update Firmware M220 User Manual Page 27 Covaris 6 3 3 Log in and Out of SonoLab To log in as Administrator e Click on the Enable Authentication See Figure 5 5 check box Enter user name and password provided by Covaris Customer Service Click OK to log in To log in as Operator Specialist e Click the Log In button See Figure 5 5 Enter the username and password provided by Administrator To log out User e Click the Log Out Button User will be logged out This is especially important for users who logged in as Administrator or Specialist Otherwise anyone who walks up to the system will be able to operate the system with elevated privileges To disable authentication Only the Administrator can disable authentication Uncheck the Enable Authentication tic and log out 6 3 4 Add a New User To add a new user to SonoLab 7 you must first Log In as an administrator e Click the User Administration button See Figure 5 5 The User Administration window will appear See Figure 5 6 e Click the Add New User button User Administration Form User Name Password Role Last Logged In Last Edited On Last Edited By Created On Created By admin Sonolab Administrator 01 31 13 11 0 11 05 09 11 2 N A 11 05 09 11 2 N A operator operator Operator 01 31 13 10 5
56. wnloading and installation instructions M220 User Manual Page 10 Covaris Turn on the computer Turn on the power switch located at the back of the M220 instrument Wait at least 10 seconds to allow the instrument to initialize Double click the SonoLab 7 icon on the desktop to start SonoLab An animated splash screen will be displayed followed by the SonoLab 7 Run screen as illustrated in Figure 2 5 NOTE By default user authentication is disabled The user has a default setting in which they can run and edit methods See User Authentication Section 6 3 2 to create user types and to take advantage of the software s full capacity amp Sonolab 7 2 Run History Setup Maintenance About Test Method 1 Notes w Required Run Time 00h 01m 47s Instrument Status Min C Setpoint C Max C Status System Check Override Light 18 0 20 0 220 Water Temperature A Water Level Q Treatment 25 sec Door ha Peak Power Duty Factor Cycles Burst ON 50 0 20 0 200 Treatment 32 sec Peak Power Duty Factor Cycles Burst 50 0 20 0 200 Treatment 50 sec Peak Power Duty Factor Cycles Burst 50 0 20 0 200 Connected X Connected to Instrument M220 COM7 Figure 2 5 The SonoLab 7 Run Screen 2 5 Connect SonoLab to the Covaris System SonoLab will automatically connect to the instrument
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