Model 11 Syringe Pump Series Manual
Contents
1. 10 Protecting Small Fragile Syringes 10 Appendices RE Aa SDGCIICALIONS 11 Table of Popular Syringe Diameters 12 Table of Minimum aximum Flow 13 2 Harvard Apparatus Syringe Pump Model 11 General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency W hile the flow will stay calibrated the peak will vary Warranty arvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PART2. 13 connector on the other Use only an approved AC line cord with a molded IEC 320 13 connector 3 main power switch located directly above the line cord on the rear panel display will now illuminate indicating that the power connections are correct The display will indicate POWER FAIL this is normal as the pump indicates on the display if power was disrupted since last use The procedure for loading syringes is the same for all models of the Pump 11 Model 55 1199 Single Syringe Pump Model 55 4154 Dual Syringe Pump Location Requirements for the Syringe Pump e sturdy level clean and dry surface e Minimum of one inch 2cm clearance around the pump e Adequate power supply Room temperature 4 C to 40 C 40 F to 104 F e Relative humidity 20 to 80 e ventilated room Loading the Syringe 1 Release the syringe pusher by pressing the bronze button on the side of the pusher W hile holding the bronze button in slide the pusher to the left Raise the spring loaded syringe retainer and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place M ove the pusher so it makes contact with the syringe plunger 2 Harvard Apparatus Syringe Pump Model 11 Fuse Replacement Fuse Replacement M ake sure the power cord is disconnected from the main supply before servi
3. 8 p s i with 50 60 cc syringe 100 p s i with 1 ml syringe 0 9 step angle motor 1 pulse in 27 6 sec 200 steps sec 0 8 um pusher advance per motor step 0 7 um min 15 mm min 1 to 16 384 Typically lt 150 Typically lt 0 05 ohms 95 240 VAC 50 60 hz 15 W 0 25 A fuse 11 4 x 22 9 x 11 4 cm 4 1 2 x 9 x 4 1 2 in 2 1 kg 4 6 Ib Harvard Apparatus Syringe Pump Model 11 Appendix B Syringe Inside Diameter Terumo Stainless Steel SGE Size Diameter Size Diameter Scientific Glass 8 95 8 cc 9 525 mm Engineering 5 13 00 20cc 19 130 Size Diameter 10 15 80 50cc 28 600 Er 20 20 15 100 cc 34 900 a 50 1 03 30 23 10 60 So E 100 1 46 Becton Dickinson 250 2 30 SSS SSS SSeS Plastic Plasticpak 500 3 26 Sherwood Monoject Size Diameter Gaal 461mm Plastic 1 4 78 25 7 28 Size Diameter 3 8 66 5 10 30 tcc 4 65 mm 5 12 06 10 14 57 3 894 10 14 50 6 1270 20 1914 12 15 90 30 21 70 Hamilton Microliter 20 20 40 Series Gastight 5 sa Size Diameter 140 38 40 Air Tite All Plastic 5 0 103 mm Size Diameter 5 2 2 5 9 60 5 0 3257 Popper amp Sons Inc 5 0 12 45 10 0 460 Perfektum Glass 10 15 90 25 0 729 Size Diameter a 5 50 1 031 0 25 3 45 mm 50 29 00 100 1 46 0 5 3 45 i 250 2 3 1 450 accuackesedeenscced 500 3 26 2 8 92 3 8 99 Somen 1 0 ml 4 61 mm
4. key T he previously used diameter will appear on the display The underline cursor will appear under the left most digit or decimal point The A V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired diameter is displayed press the ENTER key to place this value into memory Enter Flow Rate Range Choose your flow rate units ether microliters or milliliters per minute per hour From the initial RATE VOL CONFIG menu using the A or V key move the underline cursor under the CON FIG mode menu prompt Press the SET key to enter the CONFIG mode Pressthe SET key again to move the underline cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the A V keys to scroll the four flow rate choices ml min ml hr or ul hr Once the desired flow rate units are displayed press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return totheRATE VOL CONFIG menu Enter Flow Rate From the initia RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on thedisplay he underline cursor will appear under the left most digit or decimal point The A V keys
5. provided for periodic lubrication of the lead screw A small bottle of oil is provided for periodic lubrication of the two steel guide rods It is important to keep these guide rods clean and oiled Protecting Small Fragile Syringes The Pump 11 will hold microliter size syringes down to 0 5 size These small syringes have fine wire plungers that may be damaged if allowed to bot tom out The Pump 11 is equipped with an adjustable stop collar on one of the guiderods Usethe provided hex wrench to position and lock the stop col lar to prevent damage to small syringes Harvard Apparatus Syringe Pump Model 11 Appendix A Pump 11 Specifications Type Syringe Size for Pump 11 with Holder For 1 Syringe 2 Syringe Flow Rate Range Minimum Maximum Calibration Display Nonvolatile Memory Maximum Force Maximum Pressure Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Voltage Range Power Size HxWxD Weight Microprocessor single or dual syringe infusion only Plastic or glass From 10 ul to 50 60 From 0 5 ul to 10 ml 0 0014 with 0 5 ul syringe 26 55 ml min with 50 60 ml syringe Automatic enter syringe size up to 35 mm Inside Diameter 2 line 16 character vacuum fluorescent display and green run led Stores diameter rate and configuration settings 8 kg 17 bb
6. 03 0 0005 0 0010 0 0013 0 0017 0 0028 0 0016 0 0040 0 0078 0 0198 0 0395 0 0794 0 1970 0 3959 0 7918 1 974 2 794 3 952 7 909 14 97 19 88 26 56 45 64 U S A Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 Telephone 508 893 8999 Toll Free 800 272 2775 Fax 508 429 5732 e mail bioscience harvardappartus com Warner Instuments Inc 1125 Dixwell Ave Hamden CT 06514 Phone 203 776 0664 Toll Free 800 599 4203 Fax 203 776 1278 Web www warneronline com Canada Harvard Apparatus Canada 6010 Vanden Abeele Street Saint Laurent Quebec H4S 1R9 Phone 514 335 0792 Toll Free 800 361 1905 Fax 514 335 3482 Toll Free Fax 800 335 0792 e mail harvardcanada compuserve com U K Harvard Apparatus Ltd Fircroft Way Edenbridge Kent TN8 6HE Phone 01732 864001 Fax 01732 863356 e mail harvard twins co uk France Ealing S A R L 6 Avenue des Andes Miniparc Bat 8 91952 Les Ulis Cedex Telephone 33 1 64 46 00 85 Fax 33 1 64 46 94 38 e mail harvard isp fr Germany Hugo Sachs Elektronik Harvard Apparatus GmbH Gruenstrasse 1 D 79232 March Hugstetten Germany Phone 49 7665 9200 0 Fax 49 7665 9200 90 E Mail HSEMain aol com Web www hugo sachs de
7. 10 14 70 Size Diameter 5 10 3 20 19 58 10 ul 0 460 mm 10 14 57 30 22 70 25 0 729 25 23 0 50 29 00 50 1 031 50 32 6 100 35 70 100 1 460 250 2 300 500 3 260 Harvard Apparatus Syringe Pump Model 11 Appendix C Flow Rates 10 ul 25 ul 50 1 100 ul 250 ul 1 000 1 1ml 2 5 ml 3 ml 5 ml 10 ml 20 ml 30 ml 50 ml Max 0 21 0 33 0 46 0 73 1 03 1 46 2 30 3 26 4 61 7 28 8 66 10 30 14 57 20 05 23 10 26 70 35 00 0 0061 0 0149 0 0289 0 0728 0 1448 0 2909 0 7218 1 451 2 900 7 232 10 24 14 50 28 97 54 86 72 81 97 27 167 2 98 58 243 4 473 0 1 191 2 371 4 765 9 999 9 999 9 999 9 999 9 999 0999 9 999 9 999 9 999 9 999 9 999 0 0002 0 0003 0 0005 0 0013 0 0025 0 0049 0 0121 0 0242 0 0484 0 1206 0 1706 0 2413 0 4828 0 9142 1 214 1 622 2 786 1 6430 4 057 7 883 19 85 39 52 79 41 197 0 395 7 791 8 1 974 2 794 3 952 7 909 91999 9 999 9 999 9 999 0 0001 0 0001 0 0001 0 0001 0 0002 0 0003 0 0008 0 0015 0 0029 0 0073 0 0103 0 0145 0 0290 0 0549 0 0729 0 0973 0 1672 0 0985 0 2434 0 4730 1 191 2 371 4 765 11 82 23 75 47 50 118 4 167 6 237 1 474 5 898 6 1 192 1 576 2 738 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0002 0 0002 0 00
8. ICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and isnot for clinical use on human patients NOT FOR CLINICAL USE ON HUMAN PATIENTS CAUTION 2 Harvard Apparatus Syringe Pump Model 11 Introduction Theory of Operation Thenew Pump 11 is designed as a
9. Model 11 Syringe Pump Series User s Manual Table of Contents 1 SUBJECT gt Tessem 1 eneral Information Warranty Repairs 2 PER CUA crete 3 gt 3 3 Pump 11 Front and Rear V 4 5 gt Initial Setup amp Location Requirements 5 Loading the Syringe 5 A SEE RE 6 a a nar 7 Getting Started 7 z Tam PUmMpON 7 Function Keys and Run Indicator 7 Entering Syringe 8 Entering F low Rate 1 1 0 0 0 8 Entering Flow 8 Z Press E N 8 C heck Syringe 8 Advanced Features 9 VolumeModeuaunannandannuimadknunndsa dan 9 Power Fallu e uk dad 9 Display ann 9 Changing Rates gt 10 04 1
10. anced Features 0003 gt D 4 wo wo lt 5 gt 3 L 6 Display Intensity For varying light conditions four levels of intensity can be set on the vacuum fluorescent display set the desired display intensity at the VOL CONFIG display move the underline cursor using the A or V key to CONFIG Pressthe SET key to enter theCONFIG mode options Usethe A V key to scroll the CON FIG options until you reach the SET INTENSITY Press the SET key again moving the underline cursor to the right Use the A V key to scroll the SET INTEN SITY choices 1 2 3 or 4 4 ishighest intensity 1 is low est intensity Press the EN T ER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INT EN SITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until new rate is entered Except for volume mode the flow rate can be changed while the pump is running As soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in vol ume mode the pump must be stopped first Maintenance No special maintenance is required other than keeping the pump 11 clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is
11. are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired rate is displayed press the ENTER key to place this value into memory Press RUN Press the RUN STOP key to start pump and begin pumping The Run Indicator Green LED above the RUN STOP key will light when the pump is on and pumping Check Syringe ften The Pump 11 will not shut itself off when the syringe is empty or is otherwise overloaded It is prudent to check the syringe from time to time 2 Harvard Apparatus Syringe Pump Model 11 Operation Advanced Features 1 Volume ode The Pump 11 can be set to dispense a precise volume and then stop To activate the volume dispense mode target volume must be set set target volume at the RATE VOL CONFIG display move the underline cursor using the A or V to VOL Pressthe SET key to enter the VO set mode Use the A or V key and the SET key to display a target volume from 00 01 to 99 99 Volume units are ether ml milliliters or ul microliters Target volume units are established in the CON FIG SET UNITS mode example if your pump ing units are ml min or ml hr then the volume dispense units will be 5 Press the ENTER key to sdect the desired target volume Exit the VOL mode by pres ing the ENTER key Once you pres the RUN key the pump will run until the target volume is deliv ered The display will show the actual volume dispe
12. cing the fuse Fuse Replacement Procedure Turn off power remove power cord from power module Use straight blade screw driver pry open access door Remove fuse holder remove fuses from holder Replace fuse s and fuse holder Replace holder with correct voltage marking is showing through access door window For continued fire protection replace fuse only with 250V fuse of the specified type and rating T 1 4 A 250V Fuse not replaceable by the operator is located on the power supply Fuse F 2 0A 250V Screw Driver Fuse Fuse gt i 50 WARNING USE IN MANNER NOT SPECIFIED BY THE MANUFACTURER MAY IMPAIR THE PROTECTION PROVIDED BY THE EQUIPMENT 2 Harvard Apparatus Syringe Pump Model 11 Operation Getting Started Getting started 1 Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate POWER FAIL thisis normal as the pump indicates the dis play if power was disrupted since last use Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right DOHORA RUN STOP This turns the pump motor on and off ENTER This key enters the data that is on the display into the mem
13. low cost highly precise single syringeinfusion pump capable of low to moderate back pressures The pump comes in two versions one to hold one syringe of any make from 0 5 ul to 60ml and a second to hold two syringes of any make from 0 5 ul to 10 ml The diam eter of the syringe s is entered via the keyboard and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow Non volatile memory stores the last syringe diameter and flow rate along with other configuration data The Power Failure can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright D iplay and Easy To U se Interface A new two line 16 character vacuum fluorescent display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries required to start pumping syringe Inside D iameter mm and pumping flow rates Flow rate can be changed while the Pump is running Two odes of O peration Constant Flow Rate and Volume Dispense The new Pump 11 will operate continuoudy in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode Smooth Flow micro steoping pump profiles deliver very smooth and consistent flow Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Universal Power Supply No need to change AC line switches f
14. nsed along with the target vol ume Pressthe RUN key each time you want to repeat the volume dispense If you pressthe STOP key during a volume dispense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 00 or turn off and on the pump via the main power switch NOTE In the event of a power failure the actual dispensed volume and the target volume are not retained in memory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if thePOWER ON mode was set to run Power Failure In the event of a momentary or prolonged power failure the Pump 11 can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with POWER FAIL on the display To set the power fail mode at the VOL CONFIG display move the under line cursor using the A or V key to CONFIG Pressthe SET key to enter the CONFIG modeoptions Usethe A or V key to scroll the CONFIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to theright Usethe A or V key to scroll thePOWER ON choic stop or run Press the ENTER key to sdect ather mode Exit the CONFIG mode by pressing the ENTER key again and save the POWER ON setting in memory Operation Adv
15. ory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to select which digit of the display is to be changed to move the decimal point and to move between modes Each time the set key is pressed the underline cursor below the digit or character on the display moves one step theright It is used in conjunction with the ascending descend ing keys When it the display shows the desired the correct value the set key will advance right to the next digit AV Theascending and descending keys are used to change the numbers on the display Up key makes numbers increase V Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible green LED above the RUN STOP key will illuminate 2 Harvard Apparatus Syringe Pump Model 11 Operation Getting Started 3 Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm If you do not know your syringe diameter refer to appendix for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Press SET followed by theDIAM
16. uses or wires the new Pump 11 will operate on any AC line voltage from 90 VAC to 240VAC either 50 or 60 Hz Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved Thenew Pump 11 meets all relevant European EM and Safety requirements for lab oratory equipment Introduction 2 New Pump 11 Front and Rear Views PUSHER ALLEN BLOCK HALF NUT WRENCH RUN LED 3 HE 1 a O y set diam enter X 4 no 3 H AR Pump 11 APPARATUS NG KEY PAD Q lt SYRINGE CLAMP SYRINGE HOLDER 2 ADJUSTABLE STOP 1 T174 a 250v 1 4 A 250V HARVARD APPARATUS HOLLISTON MA 0176 CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS CATH POWER POWER CORD _ SERIAL SWITCH RECEPTACLE LABEL 2 Harvard Apparatus Syringe Pump Model 11 Installation Initial Set up 1 Read the manual to become familiar with all features and functions of the new Pump 11 2 Pumps are shipped from the factory with an American line cord with a U S three wire molded power plug one end and alEC 320
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