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SOFTWARE USER MANUAL

1. Click on the Acquisition tab in the upper left corner and select a method by clicking on the button next to the method window in the lower left of the screen allo T 1254 0 0 01 20 1 0 300 2001027 UVS Software for Manual Version 2 04 Oct 2004 Page 89 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS USER MANUAL bosom Set the number of samples to 4 and the Blank Samples to 1 Enter descriptions if required and insert the Blank sample in position 1 in the cell changer and the four samples in positions 2 to 5 Start the measurement by pressing the Start button The measured values will be displayed during acquisition UVS Software for Manual Version 2 04 Oct 2004 Page 90 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yad USER MANUAL DasvTos Gm The final window will look like this PA Wavelength progran I l im le AA a ae A E Acquistion fi Method EA Reri erase a x 5 Ea 00151 01252 0533 14355 0 0797 T Ennan Certe Masae Fise l atin Note If you are not using a cell changer you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged If you repeat the measurement you will always measure the selected number of samples 3 in our example until you change the Samples parameter The system will prompt if the previously run sample data has n
2. Exponential Values Format 0 0000 W Default Alignment Enter the required decimal format in the Values Format box e g for 4 decimal places enter 0 0000 2 0 3 1 3 Title This allows formatting of the name to be used for a given axis e g Absorbance The tabs allow definition of the name its alignment relative to the axis and also the font and test appearance to be used 20 2 Titles Normally only the definition of a chart titles in the form of either header or footer text is required This can be done by selecting the required function using the drop down menu and entering the text in the lower panel 2 x Asis Titles Legena Panel walla 30 Tite Style Position Format Text Gradient Shadow R Visible 7 AdjustFrame D Slignment C Let Center Bight Text WAVELENGTH SCAN DOCUMENTATION UVS Software for Manual Version 2 04 Oct 2004 Page 111 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Restoring IU Alignment top or bottom is dependent on this selection while the horizontal position can be controlled directly from the Alignment box A custom alignment using coordinates is also possible by clicking on the Position tab The remaining tabs are used to define the font type colours frame and certain special effects such as shadowing etc 2 6 3 3 Legend The legend box is used to show which curve colour corresponds to a
3. The calculation selection permits definition of the mode of calculation to be used the user can select between either absorbance or transmission 2 4 5 2 5 Audit Trail in Wavelength Program Method f a A Click on the Audit Trail icon to view the audit trail window for a method 2 4 5 3 The Report Screen in Wavelength Program Click on the Report button in the upper left corner to open the Report window i sles A toisten E memos Feron Bele A Gn v r n Fimm E T Mane Rey Daia Fanes PET 37011 S96162 1597 pot 0 30 01 2004 wiin S 8 aarosa 99 6002 0 008 0 231 53 aes 996965 99 6047 O06 0 400 Paraneler Signsiure Method Rocherte Fenin a i Approved M Facior 10 Accepted D Signed Rey Remark Liste io The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list Data can be viewed in the right hand window by scrolling across the table using the mouse Manual Version 2 04 Oct 2004 Page 86 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Rishi oarea IU 2 4 5 3 1 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on t
4. Deleted Report __ Report Name Revision UserName gt Nitrate scan without calc No Dae tme UserName amn em OdVate NewVaue Comment EA 08 03 2003 17 38 44 Created Derived from Revision 0 2 08 03 2003 17 38 45 Report modified Detect 3 08 03 2003 17 38 46 Report modified MinAbs 08 03 2003 17 38 46 Report modified Treshold 5 08 03 2003 17 39 32 ApprovalSign Approval to Test Delete 6 08 03 2003 17 40 04 AcceptSign Accept to Test Delete 08 03 2003 17 40 37 Deleted UVS Software for Manual Version 2 04 Oct 2004 Page 113 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Boe aioli arog UV The upper panel contains those reports contained in the list by clicking on one of the reports the detail for that highlighted report is shown in the lower panel 2 6 5 Electronic Signature The UVS software supports an electronic signature process as required under the CFR21 Part 11 Rule 2 6 5 1 Signing a Report In order to sign a report the signee must have been assigned the correct permission rights for the required action E g In order to be able to approve a document the user must have been assigned Approver rights by the administrator in the Login screen Reports can be signed even though the signees are not actually logged into the system at the time Entry of the password is however mandatory for acceptance of any signature for Approval or Acceptance of a document y Click
5. the rows below 2 4 4 2 5 Curve Parameters in the Auto Rate Assay Method ke Opens an edit dialogue for defining curve parameters and related formatting In Auto Rate Assay the curve parameters can be linked to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method Formatting options using this function are described in a separate section in this document UVS Software for Manual Version 2 04 Oct 2004 UVIKON 900 XL XS Page 75 of 124 UVS SOFTWARE for UVIKON 900 XS XL wN USER MANUAL Raila raea IU YV 2 4 4 2 6 Calculation Parameters in the Auto Rate Assay Method E Click on this icon to open the calculation dialogue window for the method The calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 04 Oct 2004 Page 76 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Risch oie TUV 2 4 4 3 The Report Screens in Autorate Assay 2 4 4 3 1 The Overview Report Screen Click on the Report button in the upper left corner to open the Report
6. Formatting options using this function are described in a separate section of this document 2 4 2 2 5 Calculation Parameters in the Wavelength Scan Method Click on this icon to open the calculation dialogue window for the method Prat Calculation Parameter vase 5 xi Guns hlr ety Show dawsa z e Mores Show crosspanta C iea Rase o O Fame Pesheetechon Pages alley fe r z y Or M Abo 7 Hep By activating the Calculation box a mathematical function or and a wavelength function may be implemented The Math Function may be defined with or without a reference file E g S R Subtract reference from all samples S R Add reference to all samples 1 2 Multiply all samples with 1 2 Format 1 2 or 1 2 depends on windows To use the Wavelength Function choose the number of functions and then enter the wavelength and a mathematical function to have a calculated result E g W280 W260 W250 1 2 W300 W350 Nested functions are possible too Some of the calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method 2 4 2 2 6 Audit Trail in Wavelength Scan Method an Click on the Audit Trail icon to view the audit trail window for the method 2 4 2 3 The Report Screens in Wavelength Scan 2 4 2 3 1 The O
7. XS XL Ys dT USER MANUAL Seems Dange Sedbeere Terbruk Cheit i TU Wsca iaj xl Method Report 4 gt gt i V write protect Sg E z V Show only active methods Method Revision Used dummy 0 Method not active i Lamp change 338 NM Tungsten Lam v aa gstenLamp robert 0 Deuterium Lamp Iv Lower 300 _ robert1 0 Wavelength 300 Fitter change 1f 280 mm robert 1 i z Upper 420 nm Filter change 2 370 nm _ test 0 Wavelength test 200 0 Fitter change 3 480 nm Scat kierval f o zl ii Fitter change 4 570 mm Scan speed s00 gt nimmin Fitter change 5 710 nm Autozero at start D Auto Calculation J Calculation Abs T A amp bs S Created from famin Date f 8 12 2003 14 17 27 genat i modification jamin Date 19 12 2003 12 52 13 4 gt 11 33 30 User admin The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name
8. 2 2 4 1 4 Login blocked Clicking on this checkbox can be used to prevent a user from accessing the system temporarily Permanent exclusion of a user should be made by deleting the user completely from the list using the button 2 2 4 1 5 Additional Rights Users can be assigned the right to delete reports If this checkbox is not ticked the user is not allowed to delete reports 2 2 4 1 6 Saving data Clicking on the Password required checkbox will activate a password dialogue in order to permit saving of the acquired data This security feature can be used to identify those users allowed to save data Only the currently logged in user provided with the appropriate rights can save data Clicking on the Overwrite allowed checkbox allows the user to also overwrite previously saved data Clicking on the Create additional report checkbox allows the user to save additional reports after creation of the initial report using the same acquired data UVS Software for Manual Version 2 04 Oct 2004 Page 20 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Rishi oarea IU 2 2 4 1 7 Password expired A change of password can be forced at certain intervals as defined by this dialogue The option of never changing is also available but users should note that this option is not acceptable when operating in a controlled environment Entry of a time interval automatically produces a new box
9. Calculation Abs T Abs Created from famin Date 17 12 2003 15 46 19 penei R famin Date 17 12 2003 15 47 23 4 11 44 25 User dmin The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can be entered UVS Software for Manual Version 2 04 Oct 2004 Page 61 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL m so ee CimibH Gung Sedbeere Terbruk Chekti 2 4 3 2 1 Acquisition parameters Method not active Wavelength Delay time Averaging time Measure time Sampling rate Autozero at start Auto Calculation Calculation mode Inactivate and thus hide the current Method by checking this box Defines the wavelength to be used for the measurement The instrument will wait for the selected delay time after the MEASURE key is pre
10. If more than one blank is defined then the system calculates the average blank value for the blanks and then subtracts this from the actual measured sample values Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which opened by clicking on the button adjacent to the revision box Thermo System Surveys the temperature of the sample e Mathas Rlewition Lat Changa fg 1 21_calibraton B13 01 2004 1S ede HRE cabh ear 110 20 13134 01217 froth 1331122003 105525 Eonia cabra on 3221220010574 FT iede 017122003 132 an8 cateson 119122003 121256 l 01212 hehe decor 0 TEVA 2003 10 Se Se 091216 _caltraton 219122003 112254 001223 tess 1110 20 130625 OHH cabasson reno 019125003 11 11 53 22S besii 0 23122003 11 11 40 031217 feeds O17 122003 14 14 06 EOE kal supe 0 18122003 183 25 Onder eT ie bes Lee depi dohabeticaly This window contains a list of all available methods including all revisions of the method and the date of last modification The order of the shown methods may be selected by ticking by usage or alphabetically in the lower right corner of the screen Selection is made by highlighting the required line and then clicking on OK Cell Changer The operating mode of the cell changer and the actual position can be defined here beforestarting a measurement series UVS Soft
11. This is carried out using the Se ect Report icon Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions l i fv Range from f209 2000 to 209 2000 W Method 031218 calibration Method Revision o Name po User p x The appropriate checkboxes must be checked and where applicable either a date or name selected Clicking on OK will show only those reports which match with selected criteria shown in the list of available reports UVS Software for Manual Version 2 04 Oct 2004 Page 78 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh ff USER MANUAL Risch aire 2 4 4 3 5 The Chart Report Screen Clicking on the Chart tab will open a new screen containing the curve in a form which can be edited Fauto Rate Assay Ee ziej Ez O E Acquistion BE Method TA Repri SH a EE jaj s 25 pa B Sania o E Aba EE mp i 0 nm BE tanpe 2 20 nim Alore e l Wovelength nm Be Sad kee peronii Ady i 390 0 rm oo e E Marss Frished Lier admin ee Navigation arrows are available to move between the various reports contained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display immediately above the curve display In
12. not shown if calculation is deactivated Pratometer Orio Autoren Waetergty jrn Wer cad prizia sabraion Saee Wareniength fen Ai 46 0 0 6010 TE E E nee Frihed far R This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Number of blank samples measured as the first samples gt Autozero at start of measurement UVS Software for Manual Version 2 04 Oct 2004 Page 35 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Rishi oarea IU If Individual Multiplier Divisor is activated in the Method you may add a multiplication division factor before the measurement values Both multiplier and divisor have to be defined The factor not used should therefore be set to 1 E g if you choose the multiplier of 1 7 for a sample you have to set the respective divisor to 1 The calculation will be applied by clicking on the Calculate icon in the selection toolbar You may also define a description for the factor by clicking on the fixed row to enter description FP ed Wavelength L i i a gi 30 acyn B i E ra El rias kE A NE D i De pian Fal cbr D pis Ate i Ahi Aj 3 Abs al at 1 iis ites
13. 3 Calculation 2 4 1 3 4 Concentration 2 4 1 3 5 Audit Trail in Fixed Wavelength Method 2 4 1 4 The Report Screen in Fixed Wavelength 2 4 1 4 1 Printing 2 4 1 4 2 Importing a report from the database 2 4 1 4 3 Filtering the report list 2 4 1 4 4 Audit Trail in Fixed Wavelength Report 2 4 1 5 Calculations in Fixed Wavelength 2 4 1 6 Concentration in Fixed Wavelength 242 Wavelength SCAN asinine sennieceaniaesc senses 2 4 2 1 The Acquisition Screen in Wavelength Scan 2 4 2 1 1 Run parameters 2 4 2 1 2 System Status 2 4 2 2 The Method in Wavelength Scan 2 4 2 2 1 Acquisition parameters 2 4 2 2 2 Common Parameters 2 4 2 2 3 Calculation 2 4 2 2 4 Curve Parameters in the Wavelength Scan Method 2 4 2 2 5 Calculation Parameters in the Wavelength Scan Method UVS Software for UVIKON 900 XL XS Manual Version 2 04 Oct 2004 MAd Duso lec CrmbH Gouge Hohe Tethuk Cheit i Page 3 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 2 2 6 Audit Trail in Wavelength Scan Method 2 4 2 3 The Report Screens in Wavelength Scan 2 4 2 3 1 The Overview Report Screen 2 4 2 3 2 Printing 2 4 2 3 3 Importing a report from the database 2 4 2 3 4 Filtering the report list 2 4 2 3 5 The Chart Report Screen 2 4 2 3 6 Chart Report Screen Functions 2 4 2 3 7 Audit Trail in Wavelength Scan Report 2 4 2 4 Calculations in Wavelength Scan 2 4 2 5 Peak Detection 2 4 2 6 Joined Reports 2 4 2 7 Waveleng
14. 3 2 Titles 111 2 6 3 3 Legend 112 2 6 3 3 1 Style 112 oe ce AVOR TA ee eee eee ee ere ee eee ee er ee eres Sere i Renee cere Seer mre 113 2 6 4 1 Report Trail 113 2 6 4 2 Deleted Reports 113 AGS EIECTONG SUE sr a eater 114 2 6 5 1 Signing a Report 114 2 6 5 2 Signature Status Indications 116 2 6 5 2 1 Quick Overview 116 2 6 5 2 2 Detail Overview 117 2 6 5 2 3 Signature Audit Trail 117 2 6 5 3 Deleting a Signed Report 117 3 OPTIONS AND ACCESSORIES cccccceceeeeeeeeeeeeneenseneensenseneens 118 Si AUTOMATIC CELL CHANGER al ees eae eae endian EAER E EAEE Ei 118 ld P E E a 118 Shuts Col Changer Mode only UVSA Josssie r E EEEE 118 3 1 2 1 6 samples 1 reference 119 3 1 2 2 6 samples 6 references 120 3 1 2 3 10 samples 2 references 121 3 1 2 4 Manual operation only UVSX 122 3 1 3 Cell Changer operation in the different system mMethods ccccccseeeeeeeeeeeeeeees 122 3 1 3 1 Auto rate assay 122 3 1 3 2 Fixed Wavelength 122 3 1 3 3 Wavelength Scan 122 3 1 3 4 Time Drive 122 3 1 3 5 Wavelength Program 122 3 2 UN ThermopaCk utes resarctasbeenacaee scare xcenenpacisates etna 123 Sesle MOO i sr ne cteereeed pnp etter ATE NEEESE 123 3 2 1 1 Manual operation 123 a ee Ee ke visage tees sate cen eee re a es 123 Fda RU rir seins EE EREE EER EEE EEE RE 123 lt UVS Software for Manual Version 2 04 Oct 2004 Page 6 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MAN
15. 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Restor cine Shaa 10 samples 2 references This mode allows measurement of 10 samples 5 in the sample cell holder and 5 in the reference cell holder against 2 references gt Ref 1 in position 1 of the ref cell holder gt Ref 2 in position 6 of the sample cell holder Both holders move independently during measurement Start Beam End Measure Switching Measure Ref Sample i i ample Ref Or OF Sample Ref i lt lt lt lt Po f a Pal G Ca If more than 5 samples are selected in the 10 samples against 2 references mode the sample and reference beams are exchanged after the fifth sample has been measured This unique beam switching is possible due to the fact that both beams have a fully symmetric optical layout combined with symmetrical data acquisition electronics UVS Software for Manual Version 2 04 Oct 2004 Page 121 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YA USER MANUAL in 1UY 3 1 2 4 Manual operation only UVSX The cell changer can be moved manually using the Position spinbox in the Acquisition screen The sample position will be set automatically depending on the mode setting See above Select the required sample position and the press the Set button the cell changer will then move to the required position for measurement n trument control el
16. Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve 2 4 4 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen 2386O E The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart The function of the individual icons is described briefly below UVS Software for Manual Version 2 04 Oct 2004 Page 79 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL E i TUV Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments Opens the calculation dialogue window e amp Exports a curve to the Windows clipboard for further processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format 1 Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details a 3D Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowi
17. DHHS cabe nion TO pe TC 6 kalme COTS Wen fore D91227 benta 2 3 2 Starting a measurement Esat E Stop S55 The Start button on the main menu bar is used in all methods to start measurement of one to ten samples All parameters defined in the selected method are then used for a measurement Measurement can be stopped at any time by pressing the Stop button on the main menu bar All data measured up to that point is valid and can be stored and used for further processing 2 3 3 Recalculation UVS Software for Manual Version 2 04 Oct 2004 Page 29 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH USER MANUAL E i ITU The calculations are made automatically as part of the acquisition process in the UVS software In cases where parameters are changed a recalculation can be forced as described below Clicking on the Calculator icon on the tool bar is used throughout the software package to initiate a re calculation of one to ten samples Only the parameters in the calculate column and the display column of the acquisition parameter set are used for calculation 2 3 4 Saving data Clicking on the Save button on the toolbar allows the currently displayed data to be saved The following dialogue box appears Enter the required file name in the input window and click on the OK button Please enter name to save poe o o oo o Zo 2 3 5 Loading existing data files Data which
18. O Blank Samples fo a 0 0000 al Click on the drop down menu in Mode to select the required mode from a list UVS Software for Manual Version 2 04 Oct 2004 Page 118 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL eae 3 1 2 1 6 samples 1 reference This mode allows measurement of 6 samples in the sample cell holder against 1 reference in position 1 of the reference cell holder Only the sample cell holder moves during measurement The figure below shows the cell holder positions at start and end of a measurement if all 6 cells are measured Start End Measurement i Measurement Reference Beam i Reference Beam i m l Sample Beam Sample Beam o i t Pol G ta S 5 UVS Software for Manual Version 2 04 Oct 2004 Page 119 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Restoring 3 1 2 2 6 samples 6 references This mode allows measurement of 6 samples in the sample cell holder against 6 references in the reference cell holder Both holders move simultaneously during measurement The figure below shows the cell holder positions at start and end of a measurement if all 6 cells are measured Start End RG Measurement A Measurement Reference Beam lt j EE Reference Beam i m cstl Sample Beam Sample Beam ni en S S UVS Software for Manual Version 2 04 Oct 2004 Page 120 of 124 UVIKON
19. SOFTWARE for UVIKON 900 XS XL USER MANUAL Rc hana TUV Dividing Lines Allows definition of the format of lines used between the individual legend entries within the box 2 6 4 Audit Trail The UVS software contains an integrated Audit Trail which tracks all changes made to all documents after acquisition This trail is applied to both methods and all reports and is linked to name and revision of a document eq Click on the Audit Trail icon in any screen to view the audit trail window for the current document and revision A prompt will appear asking whether you wish to view the Report Trail which contains the current reports or Deleted Reports which contain audit trails for all deleted reports It is possible to print a report of the highlighted trail by clicking on the print icon in the top menu bar or alternatively the trail can be exported directly to Excel if required 2 6 4 1 Report Trail 101 xi IEL 3s Pw Teti O o Dae Twe Uana Aston O en f ova 08 03 2003 10 07 25 admin Created Derived from Revision 3 EJ 08 03 2003 10 07 32 admin Report modified Description Description 5 Ea 08 03 2003 10 07 32 admin Report modified Remark Description 5 08 03 2003 10 28 55 admin ApprovalSign Document Approval EA 08 03 2003 10 29 56 admin AcceptSign Document Accepted 2 6 4 2 Deleted Reports The deleted report trail screen is obtained by clicking on the deleted report selection 1 x a amp x
20. The report list in the left window will become very long with time and it may be necessary to filter the list so that only those files of interest to a user are actually shown This is carried out using the Select Report icon Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions l x fw Range from fi2os 2000 to fizos 2000 Iv Method JOs1218 calibration Method Revision Name Po User The appropriate checkboxes must be checked and where applicable either a date or name entered Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports 2 4 2 3 5 The Chart Report Screen Clicking on the Chart tab will open a new screen containing the curve in a form which can be edited UVS Software for Manual Version 2 04 Oct 2004 Page 52 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL Beena arco TUV 0 Ee eee Paes E e e sewon E veros E Renee Sox 2 A t es ae 2k 05 02 3004 10 12 40 anaiei LL pEr 7 RE oe a ee ee ee estes Curves may be displayed as overlays individual curves can be removed from the overlay if required by clicking the checkboxes shown as part of the legend on the right of the curve Navigation arrows are available to move between the variou
21. This report contains only the result information and data linked to result 2018 Printing Click on either Print small or Print extended to first obtain a print preview for the requested report Click on the Printer icon in the preview to print the report 2 6 2 Direct Printing of Curves Clicking on the Chart tab in the Report screens of modes such as Wavelength Scan gives the user a direct full screen display of the curves corresponding to the selected report The appearance of the curve may be modified using the Edit function which is described in a separate section below Prints the curve as a single sheet report Click on this icon to obtain the formatting window below This method will only print the curve itself p TeeChart Print Preview OF E4 Printer Defaut Printer Setup Print Orientation C Portrait Landscape LIL LL LLL LL PEt riaririartiaiioug 11 Reset engi If View Margins W Proportional Pee ees UVS Software for Manual Version 2 04 Oct 2004 Page 108 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ets sicko image UV The upper bar in this dialogue box controls the actual operation and selection of the printer parameters using the standard printer installation windows The left hand section of the window contains formatting functions which directly influence the print out itself e g margins detail etc Changes to these para
22. UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL T USER MANUAL Bema adarra gt 040205test E test magnum overlay i M Bi ampie 1 Sample 1 E test magnum overlay 2 02 Sample 2 a test magnurm1 0 08 02 2004 admin Sample 3 test magnum2 0 08 02 2004 admin 4 sampes Sample 5 Sample 6 Sample 7 V E Sample 7 Add data with right mouse click 2 040205test admin Sample 1 Yes 040205test oo admin Sample 2 Yes D e 040205test Sample 3 5 Sample 5 No 040205test 0 admin 040205test Sample 6 er S PaA a 040205test admin 7 Sample 7 Yes ae e a A ae a a Z e Double click to unselect 2 4 2 1 Wavelength Scan Measurement Example Scanning measurement of one blank and five samples using the cell changer UVS Software for Manual Version 2 04 Oct 2004 Page 56 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Rishi oarea IU Select the method to be used and set the number of samples to 6 Insert the samples into the cell changer and start the measurement by pressing the Start button The measured curves are displayed in real time during acquisition A 3D display see below may be used to give better clarity if the curves are very similar for each sample The final window will look similarly like this E a oF sorte E Methed GA Repor Ersa el Aten ose 28 01 Kain Photormeter Contro Auiozers T
23. a set of parameters called a Method to execute the data acquisition and measurement step The method file for an acquisition is selected from a drop down list in the main acquisition window as described below each measuring mode Simply click on the required method in the list Programming and viewing of methods is made from the Method screen for each measuring mode Existing methods are shown in a list in the left hand window clicking or scrolling through the list allows an easy overview of the current method in this list A new method is programmed by removing the write protect check box tick and clicking on the icon The available functions for each method type are described in the appropriate sections below UVS Software for Manual Version 2 04 Oct 2004 Page 31 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH USER MANUAL in 1UY 2 3 9 Saving methods The system prompts for a name for the method to be stored whenever a new method is to be created Generally methods cannot be simply overwritten because of the tracking system used for CFR Part 11 therefore it is necessary to enter a new name and confirm with OK Please enter name to save Once the parameters for the method have been entered the method is saved by clicking on the green tick in the option tool bar write protect 2 3 10 Cancel all current activities Clicking on the Exit icon in the top left hand corner of each s
24. audit trail for all changes to reports will be kept if this function is checked Chart colour Colour table for curve colours in the order in which the curves are acquired The colours may be selected individually 2 2 3 Backup This function permits a backup or archive of either all data or an extract from the original database to a database sorted by year is possible The backup can be made to 2 independent servers via network if the appropriate directory locations have been entered in the options section 210 ackup folowing files ast Backup fram famir date f 7 03 2003 16 45 16 BackUp Archive from databaze Year 2003 Archive PAPAR al Backup Clicking on the Backup button will execute a backup of the complete database to the directory locations defined in the Options menu The right hand panel will display a listing of all files which will be backed up during the process A confirmation dialogue is shown after successful execution of the backup Use ae Backup sucessful Compression rate 95 The backup simply saves a copy of the working database files to the defined locations from which they can also be restored at any time as described below UVS Software for Manual Version 2 04 Oct 2004 Page 14 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL _DusoTes GmbH IU Y PE A Restoring Backup Data Restoring of previously backed up data is possible u
25. bandwidth of the spectrophotometer Defines the operating mode of the cell changer if fitted 24 3 23 Calculation UVS Software for UVIKON 900 XL XS alculation Abs I T abs Manual Version 2 04 Oct 2004 Page 62 of 124 UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Roc hiatoary IU The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission 2 4 3 2 4 Curve Parameters in the Time Drive Method Sa Opens an edit dialogue for defining curve parameters and related formatting In Time Drive the curve parameters can be linked to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method Formatting options using this function are described in a separate section in this document 2 4 3 2 5 Calculation Parameters in the Time Drive Method Click on this icon to open the calculation dialogue window for the method WR Edit Calculation Parameter i iolxi Curve undin Smooth fv Caloulaton Show dawae 7 ir None Chart Function Show crate ports Asana i Bese fw Prskidehection Penk alley e Paa mim i 0 1000 Valley Thieshold 0 0050 fF Penile fe Actively her 300 m Factor 1 0000 he 400 for rats K tbat He
26. has already been acquired can be viewed via the main Report window in any of the measuring modes J Ei Method A Report Select the Report window by clicking on the Report button The left hand section of the window contains a list of acquired data files which can be viewed and loaded by clicking on the appropriate line 2 3 6 Data conversion to EXCEL format Data files can be converted directly to Excel XLS format to be read from Excel spreadsheet programs Excel must be installed for this function to be used The data will be transferred directly to Excel and can then only be saved and processed within Excel UVS Software for Manual Version 2 04 Oct 2004 Page 30 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL H AT USER MANUAL ee TUV Caution This data cannot be re imported into the UVS software and is not subject to any further controls by the UVS software with respect to security considerations for electronic documentation Click on the icon to transfer data to Excel Excel is opened automatically and the data entered a in a worksheet 2 3 7 Export to Clipboard Data files in graph format can be exported to the Windows Clipboard to be immediately imported into other running programs via the copy paste mechanism ig Click on the clipboard icon in the graphics toolbar and the curve is exported automatically to the clipboard for further processing 2 3 8 Methods The system will always use
27. measured gt Actual wavelength gt Actual absorbance value gt Temperature where applicable 2 4 5 2 The Method in Wavelength Program Click on the Method button in the upper left corner to open the Method window W wavelength program 031218_ylprogramt E 031218_wiprogram 04011 6_conc The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not UVS Software for Manual Version 2 04 Oct 2004 Page 83 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL ES ciclo imag 1U YV Caution If measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can then be entered 2 4 5 2 1 Acquisition parameters Delay time The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Averaging time Defines the time the instrument uses to collect data for calculati
28. measurement results if these are made using different parameters such as scan speed For this reason the UVS software requires a method to be defined in Wavelength Scan mode before a baseline can be executed Click on the selection button adjacent to the method box in order to select the correct method if this is not already displayed This method is then displayed before starting a baseline but can only be edited in the method editor itself thus permitting tracking of baseline conditions during normal operation Click on OK in order to start the baseline measurement the unit will position itself at the correct starting wavelength and then automatically start the run Baseline run Please walt Lambda 305 5 nm to 300 nm UVS Software for Manual Version 2 04 Oct 2004 Page 22 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL DusoTee GmbH Dauge Gotha Techeuk Chobhl 2 2 6 Power on Switch on the power supply to the instrument PC and monitor The Uvikon has an indicator showing that the power is on From the Windows Desktop click on the shortcut to display the start up screen and login dialogue 2 2 7 Self test and system software initialisation The self test takes about 2 minutes max and performs the following checks Program memory Data memory Tungsten halogen lamp on Deuterium lamp on Data transfer Wavelength drive VVVVV V During self test you will see the main software window on the sc
29. of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Rca IU 2 4 4 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel eudozers E Wavelength nm E Samples Defines the number of samples to be analysed Autozero Defines whether an autozero should be executed before starting a measurement Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box 3101 204 101854 0 300 2004 10184 Titi O 30 07 2004 1b This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit 2 4 4 1 2 System Status a Sample omiinanas Absorbance 400 0 c D 1122 min UVS Software for Manual Version 2 04 Oct 2004 Page 72 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SA A USER MANUAL Dantes eb TUY The lower right section of the display contains the actual status of the system and displays
30. on the Signature icon to open the following dialogue window for signing a document ioi x ocument MWame evisian Signed Fey Fw Test 1 4 ignature Date Full Mame Created 08 03 2003 10 07 25 Administrator ie Approved ii Accepted fo FO mooo Login Mame Full Marne e Password Comment UVS Software for Manual Version 2 04 Oct 2004 Page 114 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YA USER MANUAL Resetting The process of signing is automated as far as is possible in order to speed up the process and support the user a minimum number of entries need to be made The significance of the various fields in the dialogue above are described briefly below Document This box contains the name of the document to be signed together with its revision number This is entered automatically following selection of the report and the signature icon in the previous Report screen The Signed Rev box is used for later reference and contains the revision number of the signed report for a given document name Created The Created information is entered automatically from the audit trail for the selected report and is based on the time of last change and the currently logged in user who made the changes Approval The approval and acceptance areas are selectable using the check button immediately adjacent to the respective dialogues In order to approve a document ens
31. plug in the cable on the accessory board connector in the sample compartment Switch on the instrument and start the UVS software The stirrer will be recognised by the software during the initialisation process Access to the stirrer is possible in all modes using either direct control in the acquisition screen UVS Software for Manual Version 2 04 Oct 2004 Page 123 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL hotometer Control Autozera Stirrer Wavelength nit 580 0 Set Move the track bar to set the stirrer speed the stirrer will follow immediately UVS Software for UVIKON 900 XL XS Manual Version 2 04 Oct 2004 mio lee GmbH ga Hiiu Choi Dauge Soib Tes tek Witi Page 124 of 124
32. preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 04 Oct 2004 Page 69 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH USER MANUAL Ros hiatoary i IU Saving Click on the Save icon and the system will prompt for a file name Please enter name to sawe poo o ooo Enter the file name and click on OK UVS Software for Manual Version 2 04 Oct 2004 Page 70 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL T USER MANUAL Bem arara 2 4 4 Auto Rate Assay The Autorate Assay mode is only possible in conjunction with an automatic cell changer Operation is basically identical with that of Time Drive 2 4 4 1 The Acquisition Screen in Autorate Assay Select Autorate Assay from the main menu to obtain the following display BP Auto Rabe Assay 2 a mmr m etar EA hy Treh 24 ita This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement UVS Software for Manual Version 2 04 Oct 2004 Page 71
33. rer 1 j ia IEJ F T Smia Fid 0 20a D2 4 aid Sig ee ia 023 Click on the fixed row to enter description Accaistion Em SSOSO lt S lt Sst lt SCStSSTT fe merien Corina Primate Drea Audozero Wie aga rer so Sa aod as Appii Saus Wises Diii fome saersi is E Yaretengih nen 546 0 a on E E E ihing And Liar miia 2 4 1 1 1 Statistic If Calculation is activated in the Method the calculation of mean standard deviation RSD min max and the deviation percentage of the mean regarding sample no 1 will be shown If Concentration is activated in the Method a chart is shown with the concentration values and the regression curve 2 4 1 1 2 Instrument Control Autozero may be activated before starting a measurement 2 4 1 1 3 Run parameters The run parameters are entered in the lower left section of the screen UVS Software for Manual Version 2 04 Oct 2004 Page 36 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Ronee le TUV Method Acmusiion Biebie Valuer sy anasa i Sarp nenan irmy Ab 546 0 0 6010 ZE E E Samples Defines the number of samples to be analysed Blank Samples Defines the number of blank samples to be measured blank samples are always expected at the start of a run The value of the measured blank is subtracted from the actual measured value of the sample prior to calculation of concentration etc
34. screen containing the curve in a form which can be edited are CC TS scaistion BE Method GS Report Bom A ttr PER orn protect kij 2280 E30 Perera To edit in cursor mode uncheck the write protect checkbox first Navigation arrows are available to move between the various reports contained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display immediately above the curve display In Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve 2 4 3 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen aa 6 ne Sie The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart The function of the individual icons is described briefly below UVS Software for Manual Version 2 04 Oct 2004 Page 66 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ein 1UY Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments Opens the calculation dialogue window Ea F Exports a curve to the Windows clipboard for further processing G Prints the curve as a single sheet report Formatting of the report is described in a separate section of t
35. the following parameters gt Current sample being measured gt No of Readings gt Remaining Time also as progress bar gt Actual wavelength gt Actual absorbance value gt Temperature where applicable 2 4 4 2 The Method in Autorate Assay Click on the Method button in the upper left corner to open the Method window la x iE 4 E Method E Report ma gt mI V write protect BE E BA M Show only active methods eee EENE gead Method ne see a Lamp change 338 nm e E SERET Tungsten Lamp W 031217_autorateassay1 2 Deuterium Lamp Iv 031219_Htest_arat 0 Delay oo Fiterchange4 280 nm Averagingtime 10 z Fiter change 2 370 nm R 1 0 min Fiter change 3 480 nm No of Readings 5 Fiter change 4 570 nm Autozero at stat D Fitter change 5 710 nm Auto Calculation V alculation Abs T abs Wavelength Number of Wavelengths f Al No Wavelenath nm Created from fadmin Date 17 12 2003 16 20 58 fomen at AA imin Date 19 12 2003 13 31 48 Bza p P r adi The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the use
36. under which the new method will be saved Once this has been defined all other parameters can then be entered 2 4 2 2 1 Acquisition parameters Method not active Inactivate and thus hide the current Method by checking this box Lower Wavelength Defines the lower wavelength for the wavelength range to be used for the measurement Upper Wavelength Defines the upper wavelength for the wavelength range to be used for the measurement Scan Interval This parameter also called sampling interval in other instruments defines the wavelength step between two measured data points UVS Software for Manual Version 2 04 Oct 2004 Page 48 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL ee TUV Scan Speed Defines the wavelength drive speed to be used for the measurement Scanning speeds can be selected between 5 and 5000 nm min If you choose dynamic scan speed the instrument will automatically optimise the scan speed to suit the slope of your sample Autozero If checked an autozero will be made for each sample beforestarting the scan Auto Calculation If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters 2 4 2 2 2 Common Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defi
37. 20 01 2004 03 6320 eink de Sc l ais een n Dae 05 02 2004 11 87 37 F o l om i a Mian Fimea SSS l l Select the Acquisition screen insert the samples in the cell changer and set the number of samples to the appropriate value Check whether an Autozero is required before starting the measurement If so click the Autozero checkbox in the Acquisition screen Caution Before executing the Autozero ensure that the cell changer if used is actually in the correct position The Autozero will be executed at the current position which may not be position 1 Measurements with the cell changer always start from position 1 Press Start after measurement the Acquisition and Parameter screens obtained are shown below The main screen contains the measured absorbance values the result and the calculated concentration values UVS Software for Manual Version 2 04 Oct 2004 Page 104 of 124 UVIKON 900 XL XS S SOF I for UVII 900 4 dT eran Sennen pia o giaa 0 05637 0563 0000 iaa asn oae The Parameter screen contains the value used for the Factor used to obtain the result the formula used to calculate the actual concentration and also the units e g ug ml mg ml etc may be entered E 400 0 0 0000 f UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL pa eter ae TUY 2 5 6 Quadratic Calibration Calculation of concentration by quadratic regression using standards The practic
38. 900 XS XL USER MANUAL sinh ovine If peak detection has been selected the results can be seen both in the peak detection table and directly on the curve itself 2 5 2 Activity This calculation mode can be used in the system methods Time Drive and Auto Rate Assay An activity of the second reaction can be used exclusively in the system method Auto Rate Assay It is used to calculate the activity of reactions Calculation of the activity is made using a linear regression model The calculation can be embedded in the method such that it is performed automatically at run end or it can be executed manually after acquisition Selection of the parameters for the Activity calculation is made as above by clicking on the Calculator icon in either the Method or Report screens Click on the Calculator icon to obtain the appropriate dialogue box and then click on the Activity checkbox UVS Software for Manual Version 2 04 Oct 2004 Page 96 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL ee i U al Erd Calculation Parameter i Milka Cures burcin Srah fe Causon Eho deneate T a None Chast Function Shoe choc poate Aver 7 Bese Lida pipit E L I Libor aad p EE miee he j Fesk alay L Ka ii m E Atay mom C00 rar Fachor 10000 to 100 mn Unas pP Actyty 2 Alescton Mar 18 0 min Factor 10040 to 25 00 mn Camison 4 1 22 Ltt xie PF He It is possible to define th
39. E orgie 1 SSS nen D Starts 2 25 iri 2 0000 1 oe Move bounds by cursor G 2 4 0 8 WW bh Na ee BD WH BA a HG g mirn The bounds for activity calculation may be moved by the cursor with real time calculation Activities can be calculated with a mathematical function UVS Software for Manual Version 2 04 Oct 2004 Page 99 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ets silo image UV 2 5 2 1 Activity Results The results of the activity calculation over the requested time period can be viewed by clicking on the Activity tab in the Report screen This displays the actual activity value for the sample plus the calculated regression line and also the correlation coefficient In addition the Table tab will show the actual activity at each point on the curve with respect to the last value For the point n on the curve at a time t and an absorbance value of Abs the activity is calculated as Activity Abs AbS n 1 l tn 2 t n 1 2 5 3 Concentration Factor This is the most simple calculation method for concentration and is used in Fixed Wavelength The concentration factor must be determined in advance if this calculation is to be used The system uses this factor and performs the following calculation Concentration Measured Absorbance value x Factor The factor is entered as a parameter in the Acquisition screen Click on the parameter tab to enter the screen and then e
40. H USER MANUAL Rey a cei TUV ye Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments UVS Software for Manual Version 2 04 Oct 2004 Page 9 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL use Tee Gmbh Dauge Sofas Techeuk Chiti 2 2 SET UP 2 2 1 Starting Ensure that the Uvikon is connected to the computer and is switched on Then click on the UVS icon to start the software The unit will first initialise a prompt will appear for user name and password User name and password are necessary because of the requirements of CFR Part 11 and permit only certain actions for users depending on the assigned permissions Exit Register Help edl Hag Fixed Wavelength fil Wavelength Program Wavelength Scan H Time Drive SS auto Rate Assay Password m L Calibration r gt Baseline Run ZAL Option mp Backup a Login 17 29 38 2 2 2 Options Clicking on Options in the main display opens a dialogue window for entry of various parameters relating to reporting and archiving functions of the software UVS Software for Manual Version 2 04 Oct 2004 UVIKON 900 XL XS Page 10 of 124 UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL mio lec GmbH ae oie Tet fo 0 Dauge Sot Terbmik imbti TU Ced l General options The first window is the General window for definition of company name etc for inclusio
41. ON 900 XL XS Yh DuSoe lee GmbH Dau Sedbere Terhuk Cheit i Page 2 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 3 1 5 The Report Window 2 3 1 6 Quick Search Function and Row Colouring 2 3 2 Starting a measurement cs coicsveciavedereediscrrinierenarnacineiiwan Eda TRC aC OU ainidadtacacitaesaneaienvessiasiessarndeeeesemiiaten k N O eeir see eae e ese ene needs 2 3 5 Loading existing data files 0 0 0 0 ecceececeeeceeeeeeeeeeeeeeeeens 2 3 6 Data conversion to EXCEL format ccccceeceseeeeeeeeeeeees 2 3 7 Export to Clipboard seciseracdereetsrncsiviniienitaernattineveteunteness k Ee IOE A E A E AANEEN E ENE ETE kda MAIN ING TOUS eorna E 2 3 10 Cancel all current activities c cece ceeccceeeeeseeseeeeeeeeeeees 2 3 11 Activate Cursor and Manual Peak Picking 00008 A My PAPA a 6 11 o Meee ee oe eee OnE E AEA Rr ror Ont heen E emer 2 3 13 Adding Comments tO curves c ccccecceeceeeceeeseeeseeesaeesaes a ey God Wel D cs Een ee nee E Sener eee Seer ener E 2 4 1 Fixed Wavelength sicicicrctrnisdciesndscinsascionantonbsandiasnddetierateenasanees 2 4 1 1 The Acquisition Screen in Fixed Wavelength 2 4 1 1 1 Statistic 2 4 1 1 2 Instrument Control 2 4 1 1 3 Run parameters 2 4 1 1 4 System Status 2 4 1 2 Parameters in Fixed Wavelength Acquisition 2 4 1 3 The Method in Fixed Wavelength 2 4 1 3 1 Acquisition parameters 2 4 1 3 2 Common Parameters 2 4 1 3
42. RE for UVIKON 900 XS XL T USER MANUAL Bem arara 2 4 3 Time Drive 2 4 3 1 The Acquisition Screen in Time Drive Select Time Drive from the main menu to obtain the following display This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement 2 4 3 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel UVS Software for Manual Version 2 04 Oct 2004 Page 59 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL eiaa Samples Defines the number of samples to be analysed Autozero Defines whether an autozero should be executed before starting a measurement Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box 3101 2004 101854 Tasi 0 S000 2004 10 78 54 ngu O 3001 20 esa der eee Ce by urag C alphabeticaly This window contains a list of all available methods including all revisions of the method and the date of la
43. SOFTWARE USER MANUAL UVS Software for UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Table of Contents 1 GENERAL INFORMATION 00 0 tteeeeeeeeeeeeees 1A eee eas na 2188 11a eater en men eee en ree ene taper mer mene et ree ee 1 3 INSTALLATION and REGISTRATION cece 2 OPERATING INSTRUCTIONS saene E PE Rr rt oe E fd i es CONS eE Fe Ed E EAER ee o i tater es een en NE accent er aes EE EES 2 2 2 1 General options 2 2 2 2 Control options eB EE 9 Eee ee Eee eee eee Tn ne ean neem nes cn ce ee ner ee 2 2 3 1 Backup 2 2 3 2 Restoring Backup Data 2 2 3 3 Archive 2 2 4 User ANAC EINIGIN oicwsaaceinaescsernnsstsxsinsneservicerenssewtiewntiucmaenens 2 2 4 1 Password 2 2 4 1 1 User rights 2 2 4 1 2 Adding users 2 2 4 1 3 Login rights 2 2 4 1 4 Login blocked 2 2 4 1 5 Additional Rights 2 2 4 1 6 Saving data 2 2 4 1 7 Password expired 2 2 4 1 8 Password 2 2 4 2 Groups Ey OGG erine E EEEE ERE ii Ce PONO E E EATE 2 2 Self test and system software initialisation 00 2 2 8 Exiting the system software ccccccccseccseeeseeeseeeeeeeeeeeeees E PETA TA cachet bach pinion ten tei ee ee eee eee 2 3 1 er xsenasce ti ncceanntnenieieees 2 3 1 1 Acquisition Calculate Display 2 3 1 2 Recommended Operating Practise 2 3 1 3 The Acquisition Window 2 3 1 4 The Method Window UVS Software for Manual Version 2 04 Oct 2004 UVIK
44. Scrolling through the list updates automatically the corresponding fields in the parameter definition fields Parameter Definition All parameters necessary for the method are defined and displayed here Current Method History Displays data relevant to the creation and history of each method in the list ie al Bes The Report Window The Report page layout is similar for each mode and is shown below Graphical display functions are again only available in those measuring modes where graphics are actually used UVS Software for Manual Version 2 04 Oct 2004 Page 27 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Ys USER MANUAL EL Acquistion E Method Report Duso lec CrmbH a W Chri at Daue walk Oh TUY l l x Working Area Selection Bar fix G m gt gt i IV write protect Selection Toolbar User Name Rev Date Nitrate Test 1 0 22 02 2003 17 34 50 admin 7 BE Sample No 1 EE Sample No 1 gt 4 22 02 2003 17 42 13 Available Reports List Overview ve Display Area Run Details Method Rev nitrate Test 1 0 Scanintervall 1 0 nm 1000 nm nin 350 400 450 500550 600 650 700 750 Remark Signature Comments Area Signature T Details Signed Rev 17 42 46 User admin Working Area Selection Selection Toolbar Available Reports List Curve Display Area Comments Area Electronic Signature Buttons
45. The calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 04 Oct 2004 Page 63 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL Riso hda oie ai TUV 2 4 3 3 The Report Screens in Time Drive 2 4 3 3 1 The Overview Report Screen Click on the Report button in the upper left corner to open the Report window BM Time Drive I le al aopision Ml metoa E Repon Some A HO n e m T biai ie Dab Up g 01234 5 67 6 4 Remark oes Fisher i l The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item and this can be manipulated or edited as required The tabs for Activity and Peak Detection show a display in table form for the respective parameters The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 3 3 2 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The rep
46. UAL Es sicko image UV 1 GENERAL INFORMATION The user should be before using the instrument have read and understood this operating manual particularly the general warnings given below 1 1 GENERAL WARNINGS gt Always disconnect the mains plug before starting any work inside the instrument gt Any maintenance operation inside the instrument including lamp replacement must be carried out by a service engineer or an authorized person The user is normally only permitted access to the inside of the sample compartment gt Keep the sample compartment clean gt Set wavelength to zero during transport to protect the drive mechanism 1 2 CD CONTENT The CD ROM supplied includes UVS Spectroscopy Software Installation program UVS User Manual Software User Manual in pdf format Adobe Acrobat Reader Installation programs UVS Software Validation Certificate 1 3 INSTALLATION and REGISTRATION The installation and registration procedures are described in a separate document For the sake of completeness the login and setup procedures are also described in this document UVS Software for Manual Version 2 04 Oct 2004 Page 7 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ros hatoary IU 2 OPERATING INSTRUCTIONS 2 1 INTRODUCTION This section of the manual describes the various operating possibilities available using the UVIKON Please note that in all cases it is necessary to follow
47. Wavelength nm seon Set R E AA AE Sarr prs Veoekerthy rit Aba Method JRechentect a aL 400 0 0 1204 Sangis in a fier an 5 Note If you are not using a cell changer you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged Individual curves may be removed from the overlay display by clicking the checkboxes in the legend on the right of the curve If you repeat the measurement you will always measure the selected number of samples 6 in our example until you change the Samples parameter The system will prompt if the previously run sample data has not been saved beforestarting a new run A Data not saved Continue anyway Click on OK to start the next acquisition run or on Cancel to return to the original data set UVS Software for Manual Version 2 04 Oct 2004 Page 57 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL in 1UY Printing Click on the Print icon to obtain a preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page Saving Click on the Save icon and the system will prompt for a file name Please enter name to save poa o u auae Enter the file name and click on OK UVS Software for Manual Version 2 04 Oct 2004 Page 58 of 124 UVIKON 900 XL XS UVS SOFTWA
48. When the cursor is activated you may manually pick peaks by clicking the right mouse button and choose Peak Picking You can also activate or deactivate samples in the legend 2 3 12 Zooming Zooming is available for all methods where graphical curve displays e g Wavelength Scan Time Drive or Auto Rate Assay are available and is used to magnify a section of the graph Simply move the cursor on the graph to the upper left corner of the rectangle to be magnified press the left button of the mouse and move it to the right while keeping the left button pressed A rectangle will be shown on the screen indicating the area that will be magnified When you reach the lower right corner of your area release the button and the area selected is zoomed to fill the graph area You can repeat the zooming function up to the maximum resolution by repeating the selection of the zoomed area To unzoom simply position the cursor on the graph press the left mouse key move the cursor to the left and release the left mouse key or double click with left mouse key UVS Software for Manual Version 2 04 Oct 2004 Page 33 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Boe aioli arco ll UV 2 3 13 Adding comments to curves You can add a comment at any point on a curve for documentation purposes When a curve is displayed on the screen click the Activate Cursor icon in the Graphics toolbar move the mouse cursor to the p
49. XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh USER MANUAL DuSoitce Gmbtt j U S 2228 Control options The second window is accessed by clicking on the Controls tab and allows control of chart print format chart colour decimal points for diplayed absorbance values and audit trail Exit Print Print Chart as Bitmap I Left Margin fis 4 mini Chart Color Decimals Decimals tor absorbance k Ed Audit Trail Functions Error messages An audit trail for all error messages will be kept if this function is checked Report revision If checked and changes are made to a report then a copy of the original will be made and the revision number incremented If this function is not checked any change will automatically overwrite the earlier version Method revision If All methods is checked and any changes are made to a method a copy of the original will be made and the revision number incremented If Only with used methods is checked changes and modifications to a method will not be tracked under revision control until the method has been used for data acquisition and the corresponding report has been saved This is the recommended setting UVS Software for Manual Version 2 04 Oct 2004 Page 13 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL _Dusetes GmbH UY If None is checked any change will automatically overwrite the earlier version Report trail An
50. al use of this calculation mode is almost identical to linear interpolation see above One additional parameter is used with the following formula Y Ax Bx C The process of obtaining the calibration data is identical for both the Linear and Quadratic calibration methods until selection of the calibration order is made Select the Quad function in the Calibration parameter dialogue Calibration parameter x Orde Linear Quad Cancel Help The calibration curve will now look like this W calibration Fixwavelength B 18 x EL 2 8 Acquisition E Method Report oe Chart y 0 764385x70 205950x0 000000 2 1 000000 0 1 14 47 48 Finished User admin UVS Software for Manual Version 2 04 Oct 2004 Page 106 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ein IU 2 6 REPORTING This section deals only with the possibilities for formatting and printing reports in the UVS software The main focus is on the treatment of curves since all table format printouts are pre defined 2 6 1 Printing The UVS software offers several possibilities for printing and formatting the various reports and data available from the system Individual print outs are possible for method parameters individual results e g peak detection complete reports and curve data Each window contains its own Printer icon which gives a preview of the print out Within th
51. avelengths Wavelength nm 1 Elon The required number of both calculations and wavelengths to be used must first be defined using the two spin boxes at the top of each entry window This definition will open the appropriate number of lines where the actual values can be entered directly Calculation Wavelengths are defined for calculation purposes as W n or w n respectively where n corresponds to actual number for a given wavelength as shown in the lower window In the example above the following calculations will be performed 1 Wavelength number 1 e g at 400 nm is added to Wavelength 2 2 Wavelength number 3 e g at 600 nm is added to Wavelength 4 3 Wavelength number 4 e g at 700 nm is divided by Wavelength 1 4 Wavelength number 2 e g at 500 nm is multiplied by Wavelength 3 Decimals The number of decimal places to be used for the calculation can also be defined This number should generally be consistent with the actual measured result e g by measurements to 4 decimal places selection of a much higher number for the calculation result will not increase the accuracy of a measurement or result and could produce rounding errors UVS Software for Manual Version 2 04 Oct 2004 Page 85 of 124 UVIKON 900 XL XS UVS Software for UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Imo lec GmbH x mre Techruk Dauge Sofi Teethruk Ohobhi 2 4 5 2 4 Calculation alculation Abs f oT Jabs
52. be opened 2 4 1 4 3 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only those files of interest to a user are actually shown This is carried out using the Select Report icon Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name UVS Software for Manual Version 2 04 Oct 2004 Page 43 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Rishi oarea 1UY Select Acquisitions lw Range from 13 09 2000 to 13 09 2000 I Method ozi 218_ calibration Method Revision Name User The appropriate checkbox must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports 2 4 1 4 4 Audit Trail in Fixed Wavelength Report Click on the Audit Trail icon to view the audit trail window for the report 2 4 1 5 Calculations in Fixed Wavelength Fixed Wavelength contains four basic calculation methods for final result determination gt Linear regression gt Quadratic regression gt Blank gt Factor In Fixed Wavelength the Blank and Factor calculations are executed automatically without the need to create a calibration file Blank The measured values for sample blank is subtracte
53. creen will return automatically to the start up screen Clicking on the Stop button in the acquisition screen will cancel any currently running data acquisition etc Caution If you exit without having saved your data on disk you cannot restore the data A message will warn you if this situation occurs 2 3 11 Activate Cursor and Manual Peak Picking a The Activate Cursor icon can be used in Wavelength Scan Time Drive and Auto Rate Assay methods to quickly overview values of the sample curve on the screen In the Report Overview window click on the chart tab to obtain the curve display Click on the Activate Cursor icon A vertical cursor can now be moved across the curve graph and the values for the wavelength are continuously displayed on the cursor line The arrow used to move the cursor is also used for the position of the Y axis and values for the current Y position are continuously updated in the legend window adjacent to the curve Additionally by clicking on the right mouse button you may add new comments edit and delete comments UVS Software for Manual Version 2 04 Oct 2004 Page 32 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Rin ti ae 1OUYsY EEE eee sdl xl a G TE apen B Method EA Remon Benz Z d HE n 4 s H as n lE a OS 2 20S EE P B oiso 2cee7 ane P BR i c ane P B 1 04520 ane E 9 23 41027 Abs 20 4D 4 8 AW AM at ede ae eee fiati Liar frinn
54. d column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can then be entered UVS Software for Manual Version 2 04 Oct 2004 Page 73 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH USER MANUAL Ronee TUV 2 4 4 2 1 Acquisition parameters Delay time The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Averaging time Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Measure time Defines the actual duration of the measurement in minutes Sampling rate Defines the number of data points per minute for the measurement Autozero at start Defines whether an autozero is to be executed beforeeach sample run Auto Calculation If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters Calculation mode Calculation and curve portrayal can be in either Absorbance or Transmission units Wavelength mode Defines the amount and the wavelengths to be used during measurement 2 4 4 2 2 Common Parameter
55. d from the measured value of each sample measured Factor The actual value obtained from the measurement is always multiplied by the factor parameter This is accessible by clicking on the Parameter tab in the acquisition screen The actual calculation is based on the following formula R F VM sample VM blank Where R Result F Factor and VM represents the measured values obtained for sample and blank respectively UVS Software for Manual Version 2 04 Oct 2004 Page 44 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL eee UWV Recalculation after for example changing the factor value is possible at any time by clicking on the Calculator icon 2 4 1 6 Concentration in Fixed Wavelength In simple cases the Result obtained above can be used as a concentration value if the Factor has been defined and zero concentration corresponds to zero absorbance Using the calibration function it is possible to calculate concentration values based on a previously determined calibration curve This method offers the advantage that any absorbance offset is compensated for and that the final result is given in the desired units in the report print outs The calibration uses the value obtained for the Result see above and converts this value to a corresponding concentration value in units which are defined as part of the calibration file A more detailed description of the Calibration function is given in a s
56. d red PY colibration fevavelength f r The data for a calibration can be viewed at any time by selecting the required report in the Report screen Click on the Chart icon and the calibration curve will be displayed together with the regression values y 0 946622x 0 003412 R 0 999946 mg ml UVS Software for Manual Version 2 04 Oct 2004 Page 103 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL Resistor cine IU 2 0 08 Using a Calibration Curve The calibration curve can now be used to measure a series of unknown samples Exit the Calibration mode and select Fixed Wavelength and then select the Method screen Here you should click the Active checkbox in the Concentration and then select the required Calibration as Reference data Save this method and the screen should look like this Praed Wavelength ole E weta protect i 3 F Show ony active methode r R A Lamp change 3380 nm Concentration fe Relahenoe nami Rev eee ecient rengi 400 0 nm CeuterumLanp fF Delay op Fiter change 1 280 nm averaging time ip z Filer change 7 30 mm Dsomais TA Filer change 3 480 nm Abs IAT abe Fiter change a 570 nm Renicates fi Te Fifer change S 710 nm readin hipan Dagar f p Cadoutation f Celi changer ilar Wiel Function e Exchide rows Taliti L ay Cae Tergetveue 0 00 feiskbrasond om Created tom acanin pate
57. defined under Math Function This function depends on the grid of absorbance values Enter the calculation desired Examples A1 A2 A4 A3 F A1 A2 A1 1 A2 1 F Table Matrix Example I Abs2_ Abs3 Abs4 Result Abs1 Table Matrix Example Il Abs2_ Abs3 Result The first number indicates the different measurements of one sample the second number indicates the sample number E g A2 1 is the first replicate of the first sample A1 2 is the first measured value of sample no 2 F factor given with the acquisition window Other operators sqr may be entered Enter the rows to be excluded The calculation of results mean std dev will not use these rows for calculation This is a value for the statistics window The deviation will be calculated with the mean value and the target value Calculation and Concentration checkboxes cannot both be ticked at the same time An error message will be shown during saving of the method Manual Version 2 04 Oct 2004 Page 41 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Ricca bdo oie IU 2 4 1 3 4 Concentration oncentration v Reference name es If the Concentration checkbox is ticked the system will use the defined calibration reference for the calculation of the concentration values based on the results of the measurement By clicking the button next to the Rev Box you can select the calibration file t
58. e always expected at the start of a run The value of the measured blank is subtracted from the actual measured value of the sample prior to calculation of concentration etc If more than one blank is defined then the system calculates the average blank value for the blanks and then subtracts this from the actual measured sample values Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box LT x Method Revision Last Changs H Recher ni 200g 101854 Tasi O S01 S04 10 754 nie O 3001 200d 1a Onder Ce ey uaga f alphabetically This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit UVS Software for Manual Version 2 04 Oct 2004 Page 82 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL TTA USER MANUAL inh vir j U ae 2 4 5 1 2 System Status The lower right section of the display contains the actual status of the system and displays the following parameters gt Current sample being
59. e curve display these are described in more detail below The status windows shows the current or last instrument status Method amp Accessory Control Enter the method name and the number of samples to be analysed Manual Version 2 04 Oct 2004 Page 25 of 124 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL a E in TUY The list for selecting methods may be sorted alphabetically or by the access counts The most used method is on the top 1301 20 13120 1 23122003 1055 2 22125005 1S a 017123003 13a 6 119122003 13125 D 16122003 10 Se 219122005 11224 1 1301 20 13064 019125003 17 17 53 0 23122003 11 11 40 D 17 12 2003 14 14 02 D 1B 2 AOS 1 SS 25 03121 kal au wen Sa be sateen The sample description can be imported from a text file from the explorer via drag and drop Select the text file in the explorer and drag it with left mouse button pressed to the description grid Then release the mouse button the text is dropped into the grid Wh rixed Wavelength Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Gre Typ ce Desktop sample 1KB Textdatei E AA Eigene Dateien EAE Arbeitsplatz Description E H 314 Diskette 4 a 3 Lokaler Datentr ger C sample 2 WIN_NT BT Textdatei B C Documents and Settings Ge ndert 26 09 2004 17 55 H Dokumente und Einstellun Gr e 48 Byte 2 Program Files 7 a a Attribute Normal UVS Software for Manual Versi
60. e Method or Report screen in order to obtain the calculation dialogue PMedit Calculation Parameter I 5 x Curve undian Smooth Me Caloulaton Show dewvalre T GLE Chant Function Show ceps Anas C Berei fe Pakodebechon mPas Aala C Paak mim is 0 1000 alley Thieshold 0 0050 CP Peoia fe Actively from 300 mi Factor 1 0000 he 00 fer Uras So xea Zue The functions available for manipulation of curve data are the following Derivatives up to 4 derivative Cross points Smoothing Mathematical calculations on a complete curve Peak detection Activity VVVVV V UVS Software for Manual Version 2 04 Oct 2004 Page 93 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Ess ciclo image UV ESLL Derivative The software allows direct calculation of derivative orders 1 to 4 for Wavelength Scan and Time Drive data A separate optimised calculation algorithm is used for each order therefore the quality of the result will be much improved if you calculate for example a fourth derivative directly from a loaded zero order curve To display a derivative click on the Show derivative checkbox which will open the spinbox for selection of the required order Set the required derivative order in the spinbox and click on OK The curve display will automatically change to show the new derivative curve Celie Show cross points Selecting the Show cross points parameter
61. e Method window 5 18 xi E meroa E Report Z m d V write protect JV Show only active methods Revision Used Method not active Lamp change 190 nm arameter Tungsten Lamp MV Wavelength 190 0 nm Deuterium Lamp V Delay oo s Filter change 1 260 nm Averaging time oa S Fiter change 2 340 nm Decimals b P Fitter change 3 470 nm Abs T Abs v Fitter change 4 540 nm Replicates 0 Fitter change 5 700 nm Individual Multiplier Divisor J7 alculation Math Function Exclude rows Statistic vd uality Control Target value 0 00 oncentration J7 Reference name Rev Created from Date Doo Remark Last modification l C from aoe 4 A 09 51 08 User dmin The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can be e
62. e in use Zale Recommended Operating Practise Each measuring mode uses Acquisition parameters which must be pre defined in a Method The Method also contains the data necessary for the final display of the results in the form of a Report The Method is the key to maintain a trace to all results and together with the different reports is subject to a strict revision control including an audit trail The method to be used can be selected from within the Acquisition page where also the naming and number of samples to be analysed can be defined For routine testing the various Methods to be used will normally have been pre defined and therefore it should not be necessary for users to access the Method screen All necessary entries for simple routine operation with a series of samples can therefore be made directly in the Acquisition area A result report can also be previewed and printed directly from the Acquisition area and is therefore not necessary for the user to switch between the various main windows The individual windows for the three main operating areas are accessible via main buttons in the top section of each window and are described below UVS Software for Manual Version 2 04 Oct 2004 Page 24 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL PAR ia aS 8 The Acquisition Window mio lee GmbH Dange Sojhyme Terbruk Cheit i The Acquisition page layout is similar for each mode and is shown bel
63. e preview page the user also has the possibility to save reports under user defined names and these reports can be viewed using the viewer of the UVS software Click on the Print icon in the report screens and you will be prompted for selection of the report type required Add pak detection bo ecbended docunennt Pret extended doceri Print smel document Print table Brink activity Print pesk detection 2 6 1 1 Add peak detection to extended document By checking this function the peak detection information will also be included in the extended report 2 6 1 2 Print extended document This will give a complete report which contains also details of the complete method used and the signature box for electronic signature confirmation 2 6 1 3 Print small document This is a report which contains only the basic results of a measurement and some other limited information regarding measuring conditions etc 2 6 1 4 Print table This report contains only the table of the measurement values and other data linked to the table 2 6 1 5 Print activity This report contains only the activity information and data linked to activity UVS Software for Manual Version 2 04 Oct 2004 Page 107 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL _Pubeten Omit TU 2 6 1 6 Print peak detection This report contains only the peak detection information and data linked to peak detection 2 6 1 7 Print result
64. e section of the curve over which the calculation is to be made a factor and the units for the Activity value It is now possible to set directly the following parameters From Defines the lower starting point on the curve time scale for the range of the Activity calculation to Defines the upper starting point on the curve time scale for the range of the Activity calculation Factor Activity is calculated by multiplying the slope of the regression line Absorbance change per minute by the factor F Units Defines the units to used for reports etc e g Abs min umol min UVS Software for Manual Version 2 04 Oct 2004 Page 97 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N 7 USER MANUAL AEBS role dt Dange Sedbeere Tetheuk Chekti Using the above example the curve obtained would appear so M E Series 0 min All curve calculation functions described above can also be used to modify curves used for the activity calculations For example it would be possible to subtract a pre determined Blank value from the curve and then multiply it by a different factor in this example the formula could be 2 S 0 2 This would give the following result M E Series 0 UVS Software for Manual Version 2 04 Oct 2004 Page 98 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Ys USER MANUAL eee TUV An example for defining two activities would appear so EE p 1 Een B Eepe 2200 ri E
65. e signed off and is the final safety check before closing the document AN You are going to accept the document The document will be locked and no modifications are possible Continue UVS Software for Manual Version 2 04 Oct 2004 Page 115 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS a USER MANUAL eee Caution Only click on OK here if you are absolutely certain that the document in its current form is acceptable PA Ra Ww Signature Status Indications 2 6 5 2 1 Quick Overview There are various indications to show the status of a report and whether it has in fact been signed off and accepted All signed reports in the list are shown with a shaded background so that the user can immediately identify those reports in the list which have been signed and accepted The status of a signed report in the report list is shown automatically when the report is selected in the report list i le PP red Wavelength Ire SE moision EE Method Repon g A HE n 4 H write protect T Barre Rew Doi User Nighues 0 00S 01424 0 1438 gi Bi 00000 0S0 0 583 Qaida C aa 12353 1 2302 D i p 03001 2004 adin Aner i vanor 400 rm Approved fe iooi f Facio 20 Dary g d Rew Blink Vole 00007 cone fr O00000c 0 001 ss0 shea fake frame ite l The Approval and Accepted checkboxes are only checked for a signed report The Signed Rev field always carries the revision nu
66. ection of the parameter within the dialogue window UVS Software for Manual Version 2 04 Oct 2004 Page 67 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 3 5 Time Drive Measurement Example Measurement of a sample Select the method to be used and set the number of samples to 1 Start the measurement by pressing the Start button and you will be prompted to insert the sample and click on OK to start the measurement Insert the sample in the correct position and click on OK The unit will now start to measure The final windows will look like this The curve display is plotted during the acquisition and can be used to monitor the progress of the measurement If the auto calculation box has been checked the regression line for the Activity calculation will be drawn immediately upon completion of the run WTime prive eee laj xi EL sA E Method Report E Abort x 2 BB amp O v X aa Chart Activity Peak Detection Parameter ethod instrument Control Cell Changer Acquisition status values Method fors To teat eal en aie esitr gt Sample HZ Wavelength nm Abs Position Set Readings 180 550 0 0 4020 Samples f EE oo min Time 550 0 Set 00 E SS 21 08 19 Finished User admin Various tab functions in the acquisition screen allow quick overviews of the results in table format as shown below ac
67. ed with the Backup if a directory for the Archive was selected in the Database directory Re importing data from an Archive Selection is made in the Report menu of a Method e Selection of the year lists the corresponding data for the selected year e Data can be re calculated and partially combined only within a given year e In the Report a range of search All files are saved in the Database directory including sub directories Two files are created filters is available Date Text etc e The copying of data from one archive into another archive or the current data base is not possible bck File extension for the re importing using the Restore tool supplied zip File extension for the manual re import procedure using an Unzip program e g Winzip e Users and Powerusers can also call up and re process archived data if the appropriate permissions in User Management have been set UVS Software for Manual Version 2 04 Oct 2004 Page 17 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL w USER MANUAL Duct On Dauge Sofie Techruk Ohobhi 2 2 4 User management has its own login facility A login name and a password is required to enter the program The assignment of user rights password administration etc is made by the administrator in a separate dialogue box accessed by clicking on the Login key in the main display Exit 2 Passw
68. ee TUS 2 4 2 5 Peak Detection Clicking on the peak detection tab will show the list of wavelength and absorbance values for the detected peaks 2 4 2 6 Joined Reports The Joined Report option offers the possibility the overlay of spectra from different runs of the same method after the reports have been saved To use the Join Report Option click on New Joined Report icon and then choose the method that you want to join your reports from Please not that only data acquired with identical method and revision of method can be overlayed and make sure that select and enter both values a Click Icon to select Acquisition Method 2 Acquisition E Method G2 Report Ey cri if a 4 BUE m i al Mew Joined Report Save Joined Report 003 1 OF M0s2005 admin O 26112003 admin O 26112003 admin 0 261172003 admin 3 0S0 admin Select Acquisitions Range from 13 09 2000 v to 13 09 2000 z Method Method Revision Mame User Select dataset from the Overview List and then add the file by using the right mouse button The selection of datasets can be monitored in the card Joined Report during the process After the completed selection of your datasets save the created Joined Report File The overlay is saved as a normal report so that all mathematical and graphical functions are available UVS Software for Manual Version 2 04 Oct 2004 Page 55 of 124
69. eparate section within this document 2 4 2 Wavelength Scan 2 4 2 1 The Acquisition Screen in Wavelength Scan Select Wavelength Scan from the main menu to obtain the following display Wisan mmr q ale x ae d Bp oenn Ml ete Eh Pereri Ao es een oi ie That Chat Peak Detection Parameter Resat Tanie F instrument Gortrct Phootoretter Conty Auozera Wevelersth nm sos se fine hoa aeiion stabs VAE i Tineke aF irem Absorbance Ee E a Worn w m 580 0 0 0005 Sanes a Haast l UVS Software for gt Manual Version 2 04 Oct 2004 Page 45 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL w USER MANUAL Boate beha Us sil This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement 2 4 2 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel Audorena f Wavelength inm sago Set Samples Defines the number of samples to be analysed Autozero Defines whether an autozero should be executed prior to the start of a measurement Method The method box contains the
70. es 3 the required method a description of each sample and the concentration in the main Acquisition screen Clicking on the Parameter tab will allow entry of the units to be used for the result and also definition of an additional multiplier factor which will be used to multiply the value obtained to give the result The final screen will look like this BM Calibration Fixwavelength i SSS 18x En oY Acquistion W Method A Fanit Sandan i Standard 2 Standard 3 Photometer Control Audozera w Wavelength rim saoo Set Acquistion saiua Values Smee o Mivelerah tne 400 0 0 0026 ae eo Diser bein Click on Start to start the acquisition Once completed the system will automatically prompt for the type of calibration required Two possibilities are available using either a linear or quadratic regression UVS Software for Manual Version 2 04 Oct 2004 Page 101 of 124 UVIKON 900 XL XS for UVIKON 900 XS XL N dT Cahbration parameter re The Calibration parameter dialogue box can be recalled at any time by clicking on the calculation icon Click the Linear checkbox and then save the calibration under the name required 1 269 oma UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _BusorFoe Gmb TUuY Values may be invalidated by double clicking them Invalidated values and their respective measure points in the Chart are marke
71. for selection of working window e g Method Contains buttons for navigating the current list plus buttons to allow archiving or printing of reports Contains a list of all reports available used for the given measuring mode Scrolling through the list updates automatically the corresponding fields in the results area fields Shows all curves available for the current report Displays data for the samples contained in the current report Shows details relating to status of electronic signature for a report 2 3 1 6 Quick Search Function and Row Colouring In Methods and Reports a quick search function may be used to quickly find the required file Therefore click on the fixed row to enter the name of the file It will be highlighted automatically UVS Software for UVIKON 900 XL XS Manual Version 2 04 Oct 2004 Page 28 of 124 UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL ae TUV PP calibration hwavelength E Doain E ero EA peri Fail a be rl i wile protect Be A M FS TONA Trae Ck 1217 eed OUMAT iede i IHHT Twede D ONHE cntration 0 KE1218 _cobtrntion 1 TAHAE cobrir 2 IIHS cabean 3 DHE catenin airs canran 5 6001218 kal sire i fash es OTE hz g DIF hei 1 Also by clicking on the small arrow in the left corner of the fixed row the rows may be coloured 7 TE in E ea EA peri a m write geotect i BE Sh TOmHG calibration WITH cp leg
72. he preview page 2 4 5 3 2 Importing a report from the database aal Click on the import icon to access earlier reports in the extracted database A dialogue window for selection of the database will be opened import database X Year 2002 Cancel Help 2 4 5 3 3 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user using the Select Report icon d Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions l i xX fw Range from jizos2000 to jaos200 W Method 031218 calibration Method Revision _ Name Po User The appropriate checkbox must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which match with selected criteria shown in the list of available reports UVS Software for Manual Version 2 04 Oct 2004 Page 87 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL E in IU 2 4 5 3 4 Audit Trail in Wavelength Program Report Click on the Audit Trail icon to view the audit trail window for a report 2 4 5 4 Calculations in Wavelength Program Wavelength Program contains alongside the basic wavelength calculations described above two additional calculation methods fo
73. his document dealing with curve format i Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details a 3D Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowing zooming of the complete curve Click on this icon to move the complete curve display using the mouse cursor Clicking on sad this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on gt this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 3 4 Calculations in Time Drive The calculation modes for curves and activities are generally embedded in the method and are described above and in detail in the Calculation section Recalculation after for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by sel
74. ication during a working session However the restored data is automatically removed locally whenever Exit is executed Modifications made during a session are automatically added to the archive data when exiting The archive is not intended for storage of data which will be needed for comparison etc Therefore great care must be taken to select the ideal time to archive all data It is also possible to selectively control archiving by using the file based backup program module to save data before deleting that data in UVS which does not need to be archived immediately The original data can then be restored as described above and the archived data removed The location of the archived database is pre defined in the options page in the UVS software and therefore the user need only define the actual year to be archived and then click on the Archive button A prompt appears requesting confirmation AN Data from Year 2003 will be copied to archive and then deleted in origin database Click on OK to complete the archiving process UVS Software for Manual Version 2 04 Oct 2004 Page 16 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SA AT USER MANUAL in 1 Menu item Options L gt General Options Setting the directories for Backup and Archive Archive Backup Execution of Archiving in the Backup Execution of Backup in the Backup window of the main menu Prior to execution the year can be selected Is sav
75. is icon to control the depth of the 3D display using the mouse cursor Clicking on H this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowing zooming of the complete curve Click on this icon to move the complete curve display using the mouse cursor Clicking on t this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on W this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 2 3 7 Audit Trail in Wavelength Scan Report Click on the Audit Trail icon to view the audit trail window for the report 2 4 2 4 Calculations in Wavelength Scan The curve calculation modes are generally embedded in the method and are described above and in detail in the Calculation section Recalculation after for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by election of the parameter within the dialogue window UVS Software for Manual Version 2 04 Oct 2004 Page 54 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N mio lee GmbH USER MANUAL ee
76. it is only possible to have one signed report with the respective report name In rare cases it may be necessary to correct a signed report after signature and then re sign it The UVS software allows a signed report to be deleted by users with administrator rights only and the deletion is recorded in a separate Deleted Reports Audit Trail UVS Software for Manual Version 2 04 Oct 2004 Page 117 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Boe aioli arog UV A new report can be signed in the normal way as described above 3 OPTIONS AND ACCESSORIES 3 1 AUTOMATIC CELL CHANGER 3 1 1 Installation Switch off the instrument and plug in the cable on the accessory board connector in the sample compartment Switch on the instrument and start the UVS software The cell changer will be recognised by the software during the initialisation process Access to the cell changer is possible in all modes using either direct control in the acquisition screen for single measurements or integration into a method for automatic processing of samples The cell changer mode can be set as described below during either of these processes 3 1 2 Cell Changer Mode only UVSX The cell changer allows three different operation modes and the mode is selectable via the Cell Changer dialogue in for example the Acquisition screen natrument Control ell changer Autozera Mode fsa t Samples i al i H Blank Walue
77. l Changer Autozero Mode fesa r Samples h al Blank value TS Blank Samples o a 0 0000 ai 3 1 3 Cell Changer operation in the different system methods Be les ba Auto rate assay In this method the instrument can measure up to ten samples simultaneously when the cell changer is in use Measurement starts with the first reading on sample 1 The cell changer then moves to the next position and measures the first reading on sample 2 This process continues until all selected samples parameter No OF SAMPLES have been measured once The instrument will then continue measurement with the second reading on sample 1 and all other samples The whole process is repeated until the selected number of readings has been measured Up to ten curves depending on the selected number of samples can be displayed on the screen during measurement Oo ladies Fixed Wavelength Measurement starts with the first sample After the selected Averaging Time the cell changer will move to the second sample Only one reading per sample is measured and displayed The cell changer can also be used to measure standards in Calibration mode 3 1 3 3 Wavelength Scan The complete scan is carried out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until the selected No of samples has been measured 3 1 3 4 Time Drive The complete Time Drive is carried
78. mber of the signed report if there is no entry in this field then no signed report exists for that particular named report UVS Software for Manual Version 2 04 Oct 2004 Page 116 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL DusoTss GmbH It is also possible to partially sign a report e g only approval and in this case only the Approval box would be checked 2 6 5 2 2 Detail Overview By clicking on the Signature icon it is possible to see the complete details of the signature entries for the given report Select the report from the list and click on the icon to obtain the Signature information TT alolxd ocuMment MWame eision Signed Rev Fifty Test 1 4 4 iqnature Date Full Mame Created 08 03 2008 10 07 25 Administrator T Approved 08 03 2008 10 26 55 Administrator Document Approval f Accepted 08 03 2008 10 28 56 Administrator Document Accepted 2 6 5 2 3 Signature Audit Trail The signature details are also recorded as part of the audit trail process and can be viewed in the report trail mron T I x FW Test 1 fe 08 03 2003 10 07 25 Created Derived from Revision 3 08 03 2003 10 07 32 Report modified Description Description 5 al 08 03 2003 10 07 32 Report modified Remark Description 55 4 08 03 2003 10 28 55 ApprovalSign Document Approval EA 08 03 2003 10 29 56 AcceptSign Document Accepted 2 6 5 3 Deleting a Signed Report For a given report
79. meters directly influence the appearance of the print out preview in the dialogue window These functions are explained briefly below Orientation Selection of either landscape horizontal or portrait vertical format for the print out Margins Spin selection boxes allow direct setting of the individual page margins Reset Margins Changing the preset margins automatically activates the Reset Margins button click on this at any time to reset all margins to their default values The button is not active if the margins are already set to their default values View Margins This checkbox allows the margin display in the preview to be switched on or off as required Detail Moving the scroller between normal and more increases the degree of detail shown in the curve display print out For example for greater detail on the axis display move the cursor towards more until the required detail is displayed Proportional This returns the print out to its original default settings without affecting the detail selected in the detail scroll bar 2 6 3 Editing and Formatting of Charts The software uses a built in editor which offers comprehensive formatting capabilities for both 2D and 3D reports Access to this editor is obtained by clicking on the Edit icon This icon is available in all screens including the Acquisition screen Use of the Edit facility when programming a method allows immediate control of the appearance of the result without the need fo
80. n on reports and also for the directory locations for the backup and database functions General Audit Trail Print Controis Type Firmware Accessory nstrument Serial na Serijal na Uw lamp off at program end om Porta nf Uvikon conn Thermo System come ption Company OTtice Dept Logo Directory Backup 2 Backup Archive Database UVS Software for UVIKON 900 XL XS PATH Programme Ss Database Manual Version 2 04 Oct 2004 Page 11 of 124 UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Este silo image UV Instrument The section contains data regarding the type of instrument serial number and firmware used In addition the accessories are listed and it is possible to enter also manually the serial number of the accessory Com Ports Define the com ports for uvikon only UVSX and thermo system if needed disable thermo system checkbox if no thermo system is connected After changing com ports please start UVS again Options These options define parameters which are included in the standard reports Under Logo the location of a company logo file can be defined which is included in every printed report Directory Here the location paths for the backup directories and database are defined Definition of a second backup location is not necessary but recommended UVS Software for Manual Version 2 04 Oct 2004 Page 12 of 124 UVIKON 900
81. name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which opened by clicking on the button adjacent to the revision box Method Revision Last Changs 3101 2004 1018 54 Tail 0 S00 2004 10134 neu C3001 2004 10247 Uer e Ce bey ua Soe UVS Software for Manual Version 2 04 Oct 2004 Page 46 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YA USER MANUAL Ein IU This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit 2 4 2 1 2 System Status Agen Shales vy aie gepa n pcueieeeea NY Absorbance 980 0 0 0005 Ea The lower right section of the display contains the actual status of the system and displays the following parameters Current sample being measured Actual wavelength Actual absorbance value Temperature where applicable VVVV 282 2 The Method in Wavelength Scan Click on the Method button in the upper left corner to open the Method window UVS Software for Manual Version 2 04 Oct 2004 Page 47 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900
82. nes the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp will be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted 2 4 2 2 3 Calculation alculation Aba toT abs The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission 2 4 2 2 4 Curve Parameters in the Wavelength Scan Method bE Opens an edit dialogue for defining curve parameters and related formatting In Wavelength Scan the curve parameters can be linked to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 04 Oct 2004 Page 49 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL E i IU
83. ng zooming of the complete curve Click on this icon to move the complete curve display using the mouse cursor Clicking on sat this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on gt this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 4 4 Calculations in Autorate Assay The calculation modes for curves and activities are generally embedded in the method and are described above and in detail in the Calculation section Recalculation after for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by selection of the parameter within the dialogue window UVS Software for Manual Version 2 04 Oct 2004 Page 80 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Rishi oarea TUV 2 4 5 Wavelength Program Wavelength Program mode permits measurements at different wavelengths and also calculations such as wavelength ratios to be made automatically for a set of samples The first samples measured can be defined as Blanks and the values of these samples will be averaged and then subtracted from each
84. nter the required value for the factor In this case no calibration file is required and the factor used is recorded in the report 2 5 4 Wavelength Program functions The Wavelength Program calculation functions apply only to this mode of operation and are described in the Wavelength Program section of this document One more parameter appears in the calculation column 2 5 5 Linear Calibration The Calibration mode is used to create standard calibration files for the Fixed Wavelength mode The Calibration uses a standard Fixed Wavelength method which must be created before running Calibration mode Method creation is possible in both the Fixed Wavelength and Calibration method screens A method can be used to create as many calibration files as needed the method and revision used is always linked to the calibration and is traceable The Calibration procedure is best explained using an example In this example we will use a blank and two standards The calibration can also be made using the cell changer 2 0 0 1 Creating a Calibration File Select the Calibration mode by clicking on the Calibration icon in the main screen and insert the blank and the two standards in the cell changer at positions 1 2 and 3 UVS Software for Manual Version 2 04 Oct 2004 Page 100 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Deets Ont os mre Tethruk Oh Dug Sodturmre Techouk Chet TU Enter the number of sampl
85. ntered 2 4 1 3 1 Acquisition parameters Method not active Inactivate and thus hide the current Method by checking this box Method not active E UVS Software for Manual Version 2 04 Oct 2004 Page 39 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Wavelength Delay time Averaging time Decimals Abs T Replicates Individual Multiplier Divisor 2 4 1 3 2 Vad mso Pee CimilbH T USV arameter Wavelength 1900 nm Delay 00 3 Averaging time O1 Decimals bh p Abs F367 abs Replicates lo A Individual Multiplier Divisor fe Defines the wavelength to be used for the measurement The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Defines the amount of decimals for the measurement values Selection permits definition of the mode to be used The user can select between either absorbance or transmission Defines the number of acquisitions per sample to determine the resulting mean E g Replicates 0 only one acquisition per sample Replicates 1 two acquisitions per sample By checking this box a separate dilution factor multiplier and weight factor divisor can be defined for each sample C
86. o be used The boxes show the Reference name of the selected file and its revision number Rev Note Calculation and Concentration checkboxes cannot both be ticked at the same time An error message will be shown during saving of the method 2 4 1 3 5 Audit Trail in Fixed Wavelength Method F ry ats Click on the Audit Trail icon to view the audit trail window for the method 2 4 1 4 The Report Screen in Fixed Wavelength Click on the Report button in the upper left corner to open the Report window Pred Wavelength ii jjs eee SE omision EE metoa aepo Baxi oer P piia 0020 ETE a esos E T UVS Software for Manual Version 2 04 Oct 2004 Page 42 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH USER MANUAL E i TUV The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 1 4 1 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page Import database x 2 4 1 4 2 Importing a report from the database Click on the Import icon to access earlier reports in the extracted database A dialogue window for selection of the database will
87. obtained In many cases this can be confusing for the user and generally it is recommended that once known the range should be set manually for routine operation Visible Check this box if you want an axis to be visible uncheck to remove it Invert Checking this box will invert the chart Maximum If automatic is set to off a fixed signal maximum can be defined here Minimum If automatic is set to off a fixed signal minimum can be defined here Desired increment The desired axis increment is the minimum step between axis labels It must be either a positive number or DateTime value This value will be used as the initial step value If there is not enough space for all labels then a larger increment will be automatically calculated This value defines only the increment used on the scale it is also possible to format the number of decimal places required as described below Logarithmic Check this box to obtain a chart based on the corresponding logarithm UVS Software for Manual Version 2 04 Oct 2004 Page 110 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS AT USER MANUAL pa tare TUV 2 6 3 1 2 Decimal Format The decimal format required can also be set from within the Axis tab Click on Labels and then on the Format tab to obtain the following window 2 xl Axis Titles Legend Panel wale 30 Visible Scales Title Labels Ticks Minor Position IW Behind Style Format Text Ages
88. oint on the curve where the comment should be added and click the right mouse button A small dialogue box appears offering the choice of adding editing or deleting a comment Click on a selection and the appropriate dialogue window will appear b Edit Comment _ O Curve Series 0 Comment No x Abort 7 Help Input the desired comment text using alphanumeric characters and press OK The comment will appear directly linked to the curve at the cursor position on the X axis More comments may be added by repeating the same procedure The comments are numbered and the spin selector by the Comment No box can be used when editing or deleting comments in order to select the item to be used UVS Software for Manual Version 2 04 Oct 2004 Page 34 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS AT USER MANUAL Ricoh oie al 2 4 SYSTEM MODES This section explains the parameters and gives a step by step measurement example for each system mode Many parameters are common to all methods but are explained separately for each mode in the interests of clarity 2 4 1 Fixed Wavelength 2 4 1 1 The Acquisition Screen in Fixed Wavelength Select Fixed Wavelength from the main menu to obtain the following display Fred wavin alaiz E o ggi 1 tm DEDS 0 S00 Oh i D2027 04I D Hii ii p ch ea giri i Samples of Replicates Depends on results
89. ommon Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change Tungsten lamp Deuterium lamp Filter change 1 5 Bandwidth Cell changer UVS Software for UVIKON 900 XL XS This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength When checked the tungsten lamp will be switched on and used according to the setting for the lamp change When checked the deuterium lamp will be switched on and used according to the setting for the lamp change This parameter defines the wavelength used to switch the filter Selects the spectral bandwidth of the spectrophotometer Defines the operating mode of the cell changer if fitted Manual Version 2 04 Oct 2004 Page 40 of 124 UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL m so Pee CimibH Dange Sedbeare Terbruk Cheit i 2 4 1 3 3 Calculation Calculation Math Function Exclude rows Target value Note UVS Software for UVIKON 900 XL XS alculation Math Function ee o Exclude rows Statistic E Walt Control Target value O00 Deactivate Calculation for normal acquisitions without any special calculation Activate Calculation for special calculations
90. on 2 04 Oct 2004 Page 26 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL DuSoTee Gmbt j U S 2 3 1 4 The Method Window The Method page layout is similar for each mode and is shown below Graphical display functions are again only available in those measuring modes where graphics are actually used e g Wavelength Scanning Other functions such as calibration definition are also not present in every window Mscan DD eee li x 2 Acquisition E Method 1 E Report Working Area Selection Bar A ES write protect X SAE amp Selection Toolbar Lower 380 Wavelength 350 Lamp change 338 nm Upper 750 nm Tungsten Lamp Iv Wavelength Deuterium Lamp D Scaninterval fi gt nm Fitter change 1 280 nm d Fitter change 2 370 nm Scanspeed f 000 gt niminin Fitter change 3 480 nim Autozero at start v Fitter change 4 570 nm Auto Calculation D i Filter change 5 710 nm Calculation b ga a Abs T labs gt Bandwidth 2 nm gt Existing Method List a J Run and Calculation Parameters Bd Laoi eet ee z ZEE Current Mathod History A 17 36 30 User admin Working Area Selection Buttons for selection of working window e g Method Selection Toolbar Contains buttons for navigating the current method list plus buttons to allow addition or deletion of methods Existing Method List Contains a list of all methods used for the given measuring mode
91. on of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values 2 4 5 2 2 Common parameters The right hand panel contains common and calculations parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp will be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted UVS Software for Manual Version 2 04 Oct 2004 Page 84 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Boars arco UV 2 4 5 2 3 Wavelength Program calculations The right section of the method screen allows definition of the wavelengths to be used and also the calculation modes Number of fa al calculations Calculation rn to iy Humber of fa al F W
92. ord Groups write protect admin ull Mame ii g WEer aving Data administrator Password required Overwrite alloyed Login hiocked E Create additional report assyvord expired ethod Rights Allow user to modify Never eport Rights n mmm Allow user to delete Allow user to modify aika fember of group ption Rights Allow User to modify ervice Rights x x Allow user to exeute ackup and Archive Allow user to backup Allow user to archive ka im alibration Rights x ignature Rights ermitted Actions Approval Accept UVS Software for Manual Version 2 04 Oct 2004 Page 18 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 2 4 1 Password 2 2 4 1 1 User rights Mda mso lee CimibH Gouge Sedbere Techuk Cheit i Generally three different user types are recognised with basic default permissions as described below Action Administrator Main window Checked security Visibility rights Scan tyes tyes yes yes yes yes yes yes Scan yes Autorate Assay yes yes yes yes yes yes yes yes Calibration Depends on security rights Baseline Run Option Backup Edit Security Trail Service Password required on saving data Overwrite existing report on saving data Create additional report on saving data Change Method Delete Report Modify Report Signature approval Signature accep
93. ort can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 04 Oct 2004 Page 64 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Linton IU YV 2 4 3 3 3 Importing a report from the database aal Click on the import icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened Import database X Tear 2002 Cancel Help 2 4 3 3 4 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user This is carried out using the Se ect Report icon Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions Ih Range from 13 09 2000 to 13 09 2000 I Method ozi 218_ calibration Method Revision Name User The appropriate checkboxes must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports UVS Software for Manual Version 2 04 Oct 2004 Page 65 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL a an 2 4 3 3 5 The Chart Report Screen Clicking on the Chart tab will open a new
94. ot been saved before starting a new run A Data not saved Continue anyway Click on OK to start the next acquisition run or on Cancel to return to the original data set UVS Software for Manual Version 2 04 Oct 2004 Page 91 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL in 1UY Printing Click on the Print icon to obtain a preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page Saving Click on the Save icon and the system will prompt for a file name Please enter name to save ao Enter the file name and click on OK UVS Software for Manual Version 2 04 Oct 2004 Page 92 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL in IU 2 5 CALCULATIONS The UVS software contains various calculation possibilities depending on the selected measuring mode These calculations are accessible using the Calculator icon 2 5 1 Curve Functions Calculations based on charts can be executed in one of two ways gt Post run by clicking on the Report tab in the report section and then the calculator icon in order to obtain the calculation dialogue gt Definition of the required calculations as a part of the method in which case they can be executed automatically at the end of the run Click on the calculator icon in either th
95. ouble the signal level and this would be programmed as follows Click on the Calculation checkbox and then enter the required formula in this example the formula would simply be 2 S 0 2 Caution Please note that spaces are not permitted in the formula 2 0 13 Peak Detection This calculation mode can be used with the system methods Wavelength Scan Time Drive and Auto Rate Assay Peak detection can be linked to a method and carried out during or after measurement To activate peak detection select Peak detection in the calculation parameter editor window A peak is detected when the slope of the curve changes from positive to negative a valley is detected when the slope changes from negative to positive After selecting Peak detection it is possible to set the following parameters Min Abs The Min Abs parameter defines the absolute minimum value for a peak or valley to be detected any peak or value with an absorbance value lower than the defined value will be ignored Threshold The threshold is the difference in height on the Y axis between two peaks or valleys If the difference between valley and peak is lower than the set threshold then the peaks valleys will not be detected Peak Valley The selection defines whether the calculation is to be made for peaks only valleys only or both peaks and valleys UVS Software for Manual Version 2 04 Oct 2004 Page 95 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON
96. out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until the selected No of samples has been measured a ss Wavelength Program All samples are measured at the first wavelength then the wavelength is changed to the second one and all samples are measured again This process will be repeated until all wavelengths for all samples have been measured UVS Software for Manual Version 2 04 Oct 2004 Page 122 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL i 1UY 3 2 Thermosystem Thermopack 3 2 1 Installation Connect the thermosystem or thermopack the serial line COM 1 Start the UVS Software the thermosystem thermopack will be detected S2 11 Manual operation If the thermosystem thermopack is detected an additional window is shown in the control display area of the acquisition window Instrument Control wf Thermo System sample E Reference E OO Set OO set ethoc Thermo System Method vs Sample C Reference C Reference e 0 0 0 0 Samples h eae i al Blank value 0 0000 Enter the required sample or reference value and the press the Set button the thermosystem thermopack will then move to the required temperature value If a thermopack is detected only the sample is shown 3 3 Stirrer only UVSX 3 3 1 Installation Switch off the instrument and
97. ow A graphical display is only available for those measuring modes where graphics are actually required and used e g Wavelength Scanning laix En 2 Acquisition fl Method Report Working Area Selection Bar E Stat m Abort e x Standard Toolbar R a gt 8 Ga x amp Graphics Toolbar 1 09 ek ee ee ee A NEET oo Result Display Area 0 7 0 6 o54 Abs 0 4 0 3 0 2 0 1 0 350 360 370380 390 400 410420 430 440 450 460470 480 490 500510 520 530 540550 560 570 580 590600 610 620 630640 650 660 670 680690 700 710 720730 740 750 i i IV E Series 0 Peak Detection Parameter Method Instrument Control Sipper Cell Changer Acquisition status values i BsM Wavelength nm Abs Method nitrate Test o A Autozero 7 Stop Mode s tr Sampe 1 PETN ITT Rins Position 1 Set 280 0 z Sampei 2 Method and Accessory sontrol FE Status Windows Return 580 0 Set 0 0 E 17 23 05 Finished ser admin Working Area Selection Standard Toolbar Graphics Toolbar Status Windows UVS Software for UVIKON 900 XL XS Buttons for selection of working window e g Method Contains buttons for starting and stopping measurements plus Excel export printing and saving data for the currently open document Contains all functions required for controlling the appearance of th
98. particular sample The default text used is Series N where N is the number of the sample in the batch This default text is only used when no sample description is defined otherwise the actual description will be used automatically for the legend 2 x Axis Titles Legend Panel wals 3D Style Position Symbols Format Text Gradient Shadow W Visible Legend Style Automatic Inverted Test Style Left Value M Check boxes Vert Spacing 0 E Font Series Color The Style and Position tabs are most likely to be used The remaining tabs control items such as size and form of the displayed symbol and generally the applied default values will not need changing PAS A Style Visible Unchecking this checkbox will remove the legend box from the chart Inverted Checking this box will cause the legend order to invert i e the last sample to run will be shown at the top of the box Checkboxes Unchecking this box will remove the checkboxes adjacent to the text in the box This is not recommended since the checkbox is very useful if a single curve should be removed from the overlay for any reason Legend Style It is recommended to maintain this setting as Automatic per default Text style It is recommended to maintain this setting as Left Value per default Vert Spacing Controls the line spacing used within the legend box UVS Software for Manual Version 2 04 Oct 2004 Page 112 of 124 UVIKON 900 XL XS UVS
99. r the final result determination gt Blank gt Factor In Wavelength Program the Blank and Factor calculations can be executed automatically without the need to create a calibration file Blank The measured values for sample blank at each wavelength is subtracted from the measured value of each measured sample at the same wavelength Factor The actual value obtained from the measurement is always multiplied by the factor parameter This is accessible by clicking on the Parameter tab in the acquisition screen The actual calculation is based on the following formula at any given wavelength RW F VM sample VM blank Where RW Result for wavelength W F Factor and VM represents the measured values obtained for sample and blank respectively The value RW is then used for the defined calculations for the individual wavelengths Recalculation after for example changing the factor value is possible at any time by clicking on the Calculator icon before the data has been saved UVS Software for Manual Version 2 04 Oct 2004 Page 88 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL iaa i a rrea IU YV 2 4 5 5 Wavelength Program Measurement Example Wavelength Program measurement of 4 samples using an optional automatic 6 6 cell changer Four wavelengths are used 400 500 600 and 700 nm The first sample is a Blank Define the method to be used BM wavelengt 1 program Be aE a
100. r time consuming manual manipulations A Help function is provided within the Editor and therefore this document will only describe the more common needs with respect to formatting of charts the user is referred to the Help function for additional information 2 6 3 1 Changing the scaling Axis The axis scaling is the first available tab in the Edit dialogue box when opened Separate parameters can be defined for each axis and normally users would only be concerned with the so called left and bottom axes Y and X respectively UVS Software for Manual Version 2 04 Oct 2004 Page 109 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YH dT USER MANUAL Restor cine IU 2 x Axis Titles Legend Panel walls 3D Iv Wisible Scales Title Labels Ticks Minor Position I Behind fone W Automatic fw Visible Inverted M Auto Change Maximum 1 545 M Suto Change Minimum 0 012 Change Desired Increment 0 001 r Logarithmic Log Base 10 Right Axis Top Asis Bottom Asis Depth Asis The required axis to be formatted is selected by clicking in the list in the left hand panel Above this panel there is also a checkbox which allows the axes to be removed from the chart visible or not The significance of the other parameters is described briefly below 2 6 3 1 1 Scales Automatic The software uses dynamic scaling depending on the level of the signal
101. reen with Initializing instrument displayed on the bottom status bar Exit Register Help ia Fixed Wavelength fil Wavelength Program aa Wavelength Scan 4 Time Drive SS Auto Rate Assay E Calibration Baseline Run cn Option E Backup h Login iz Lee Initializing instrument After completion of the initialisation this message will disappear and the software will prompt for user name and password for log in UVS Software for Manual Version 2 04 Oct 2004 Page 23 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Ess sicko image UV 2 2 8 Exiting the system software You can exit the system software by clicking either on the Exit icon in the top left corner or on the X in the top right corner 2 3 OPERATION 2 3 1 General guidelines 2 0 15 1s Acquisition Calculate Display The UVIKON uses an Acquisition Calculate Display scheme as shown below Acquire data for Calculate Display results of 1 to 10 samples using 1 to 10 samples using 1 to 10 samples using method parameters method parameters method parameters The system is structured to use individual measurement modes e g Fixed Wavelength which must be pre selected by the user The selection of the mode to be used is made in the opening screen which can be accessed immediately after the software has started and login has been completed This screen can also be reached at any time by exiting from the current mod
102. s The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp will be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted 2 4 4 2 3 Calculation alculation Abad FT aps The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission UVS Software for Manual Version 2 04 Oct 2004 Page 74 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 4 2 4 Wavelength Wavelength Humber of 5 Wavelengths WA Wavelength nm B00 Vad Duso lec CrmbH J eel iat b Danag it Techuk Cheit A maximum of six different wavelengths may be chosen The A of the wavelengths can be defined in
103. s reports contained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display immediately above the curve display In Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve 2 4 2 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen at ole om eh The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart Tha f Inntinn nF tha raive ial InAANna Ia Ananrihan hrinfh halaiwar Shows position of values on X and Y axis and allows to manually pick peaks and to add a edit or delete comments Opens the calculation dialogue window UVS Software for Manual Version 2 04 Oct 2004 Page 53 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL in IU Exports a curve to the Windows clipboard for further processing G Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format 1 Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details R 3D Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on th
104. sing the restore UVS application located in the UVS directory In order to restore data you must close the normal UVS software before starting the restore process a prompt will remind you of this before continuing Start the restore UVS program a prompt will appear to check that the main program has been closed x Have you closed the UYS program 7 IF not please close it before continuing with Restore Close if necessary and click on OK to obtain the restore dialogue Restore database UYS Ioj x V1 Database pat C Program Files U Se database Files restore Restore Specify the location from which you want to restore the data and click on the Restore button You will be prompted to enter your name Please enter your name This name is required for record purposes and does not correspond with the user names installed for the main UVS software Enter a name and click on OK UVS Software for Manual Version 2 04 Oct 2004 Page 15 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Boe aioli arog TU YV The restore process will be confirmed x Restore successfully Finished 33 Files of 33 restored ae De ee Archive The archive is based on data obtained within any given year e g all data collected during 2003 The data is then archived in the database and the original data removed locally Caution It is possible to restore Archive data for viewing and modif
105. ssed to start the actual measurement Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Defines the actual duration of the measurement in minutes Defines the number of data points per minute for the measurement Defines whether an autozero is to be executed beforeeach sample run If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters Calculation and curve portrayal can be in either Absorbance or Transmission units 2 4 3 2 2 Common Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change Tungsten lamp Deuterium lamp Filter change 1 5 Bandwidth Cell changer This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength When checked the tungsten lamp will be switched on and used according to the setting for the lamp change When checked the deuterium lamp will be switched on and used according to the setting for the lamp change This parameter defines the wavelength used to switch the filter Selects the spectral
106. st UVS Software for Manual Version 2 04 Oct 2004 UVIKON 900 XL XS Mhdd Duso lec CrmbH 3 sent Chu Gouge Hobe Techuk Cheit i 50 50 50 51 51 52 52 53 54 54 55 55 56 59 59 60 61 62 62 62 63 63 64 64 64 65 65 66 66 67 68 1 72 72 73 14 14 14 75 75 76 11 11 78 78 Page 4 of 124 UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL eaten amets 2 4 4 3 5 The Chart Report Screen 79 2 4 4 3 6 Chart Report Screen Functions 79 2 4 4 4 Calculations in Autorate Assay 80 LAS VERNON ENT A erie eer neti eE E bn eneryp EEEE 81 2 4 5 1 The Acquisition Screen in Wavelength Program 81 2 4 5 1 1 Run parameters 82 2 4 5 1 2 System Status 83 2 4 5 2 The Method in Wavelength Program 83 2 4 5 2 1 Acquisition parameters 84 2 4 5 2 2 Common parameters 84 2 4 5 2 3 Wavelength Program calculations 85 2 4 5 2 4 Calculation 86 2 4 5 2 5 Audit Trail in Wavelength Program Method 86 2 4 5 3 The Report Screen in Wavelength Program 86 2 4 5 3 1 Printing 87 2 4 5 3 2 Importing a report from the database 87 2 4 5 3 3 Filtering the report list 87 2 4 5 3 4 Audit Trail in Wavelength Program Report 88 2 4 5 4 Calculations in Wavelength Program 88 2 4 5 5 Wavelength Program Measurement Example 89 E CALCULA TE E sre AE ENEE EEEE E EERE EEEE EEEE NEEE 93 Cls T E a E E O 93 2 5 1 1 Derivative 94 2 5 1 2 Show cross points 94 2 5 1 3 Smoothing 94 2 5 1 4 Calculation 95 2 5 1 5 Peak De
107. st modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit 2 4 3 1 2 System Status TAn Sabie ag Sanpe o Wavelength inm Absorbance Resdngs 400 0 0 1122 h eg rJ ri Tan peii EI B The lower right section of the display contains the actual status of the system and displays the following parameters UVS Software for Manual Version 2 04 Oct 2004 Page 60 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SA A USER MANUAL Dasytes net TUY Current sample being measured No of Readings Remaining Time also as progress bar Actual wavelength Actual absorbance value Temperature where applicable VVVVVV 2 4 3 2 The Method in Time Drive Click on the Method button in the upper left corner to open the Method window laix E 9 E Method Report MS 031217 _timedtrivet gle pyle deaths a Lamp change 338 nm 031219 _HTest_td_1 o Pages TungstenLamp W Wavelength 595 0 nm Deuterium Lamp Vv Delay S oo Averaging time 25 a Fitter change 2 370 nm Measure time 50 mo Fitter change 3 480 nm Data rate k x as Fitter change 4 570 mm Autozero at start WV Fitter change 5 710 nm Auto Calculation Fitter change 1 280 nm
108. t Report yes Depends on security rights Depends on security rights Access only to print controls Depends on security rights Access only to print controls Depends on security rights moo fm Depends on security rights Depends on security rights Depends on security rights Depends on security rights Depends on security rights Depends on security rights Depends on security rights Depends on security rights Depends on security rights Only own grouped member or Only own grouped member reports or The permissions for individual users are modified by the administrator by checking the appropriate boxes UVS Software for UVIKON 900 XL XS Manual Version 2 04 Oct 2004 Page 19 of 124 UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Beca image UWV 2 2 4 1 2 Adding users In order to add a user it is always necessary to unclick the write protect checkbox and the click on the or symbols to add or remove a user respectively Click on and a dialogue box will prompt for a new user name Please enter user name Enter a name and click on OK the new user will be entered in the list and the permissions for the new user can be selected using the dialogue boxes on the right of the window 2 2 4 1 3 Login rights Clicking on the drop down arrow cause the three possible user types to be displayed as a drop down list Select the required user type from the list
109. tection 95 Ea P E O eT ERR OTT ARTES A A eet rane he Ten See 96 2 5 2 1 Activity Results 100 i Cowon FAO anina EE E AE 100 2 5 4 Wavelength Program functions ccccccccccceeeeceeeeeneece cece eeseeeseeeesaeesageeseeeseeeeeeess 100 es Ce Ge 6 eo eee ee ee ne eee eee ene enn ene enna ene peer heehee nt re ee 100 2 5 5 1 Creating a Calibration File 100 2 5 5 2 Using a Calibration Curve 104 2 5 6 Quadratic Calibration a eta etecenns tice dtinn cru dave esandevacnenanuensacshadeneiranesnndhvabianiaydeckeeniat 106 Eo ole oY S Seee ee eeen ees eke rene T enres EF oem Pree ne et neer fom Cera ner eet reece ee tee eRe eh et er eaten 107 hg FRED ceases ATE AA ENET en ainda cee an enss ageless 107 2 6 1 1 Add peak detection to extended document 107 2 6 1 2 Print extended document 107 2 6 1 3 Print small document 107 2 6 1 4 Print table 107 2 6 1 5 Print activity 107 2 6 1 6 Print peak detection 108 2 6 1 7 Print result 108 UVS Softwarefor Manual Version 2 04 Oct 2004 Page 5 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL UVIKON 900 XL XS Wh Ad Duso lec CrmbH ikem 1UY 2 6 1 8 Printing 108 ee Direct i a hen icin wes eee sei 108 26 3 Editing and Formatting OP GS iss vicesniccnsicagexsacteviniscenascrennseseesansaeressatessnniseieaeniarianss 109 2 6 3 1 Changing the scaling Axis 109 2 6 3 1 1 Scales 110 2 6 3 1 2 Decimal Format 111 2 6 3 1 3 Title 111 2 6
110. th Scan Measurement Example 2 4 3 Time Drive cts ecw ntact canwennctawuiveviene ids eed tatstie nada anannsexoiaene 2 4 3 1 The Acquisition Screen in Time Drive 2 4 3 1 1 Run parameters 2 4 3 1 2 System Status 2 4 3 2 The Method in Time Drive 2 4 3 2 1 Acquisition parameters 2 4 3 2 2 Common Parameters 2 4 3 2 3 Calculation 2 4 3 2 4 Curve Parameters in the Time Drive Method 2 4 3 2 5 Calculation Parameters in the Time Drive Method 2 4 3 3 The Report Screens in Time Drive 2 4 3 3 1 The Overview Report Screen 2 4 3 3 2 Printing 2 4 3 3 3 Importing a report from the database 2 4 3 3 4 Filtering the report list 2 4 3 3 5 The Chart Report Screen 2 4 3 3 6 Chart Report Screen Functions 2 4 3 4 Calculations in Time Drive 2 4 3 5 Time Drive Measurement Example 2 4 4 Auto Rate Fie S AY is eecsaciccniencnidadiniaiinispiamnbedesariateveedauenaietennswess 2 4 4 1 The Acquisition Screen in Autorate Assay 2 4 4 1 1 Run parameters 2 4 4 1 2 System Status 2 4 4 2 The Method in Autorate Assay 2 4 4 2 1 Acquisition parameters 2 4 4 2 2 _ Common Parameters 2 4 4 2 3 Calculation 2 4 4 2 4 Wavelength 2 4 4 2 5 Curve Parameters in the Auto Rate Assay Method 2 4 4 2 6 Calculation Parameters in the Auto Rate Assay Method 2 4 4 3 The Report Screens in Autorate Assay 2 4 4 3 1 The Overview Report Screen 2 4 4 3 2 Printing 2 4 4 3 3 Importing a report from the database 2 4 4 3 4 Filtering the report li
111. the general guidelines laid down in section 2 3 dealing with general operation Examples give a step by step introduction to all system methods and calculation modes 2 1 1 System Icons The UVS software allows direct selection of many functions via a direct click on the corresponding icon Many of these icons have the same function in different methods Saves the acquired data in the database In addition a report file will be created in the EF corresponding directory for a given method These report files can be viewed using the qrv exe viewer Print preview and printing of a report Selection mask You can select a view with date method user or name The appropriate checkbox must be checked a Transfers data to Excel Data will be transferred directly to Excel Excel must be installed for as this function to be used Note that this is not a file export and the data can then only be saved and processed within Excel Imports an earlier extracted database it Opens an edit dialogue for reporting functions Exports a curve or table to the Windows clipboard for further processing Opens the calculation dialogue window or executes a re calculation Opens the audit trail dialogue to allow viewing of the audit trail for a document Opens the signature dialogue to allow signing of a document amp amS UVS Software for Manual Version 2 04 Oct 2004 Page 8 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Y
112. tivity etc UVS Software for UVIKON 900 XL XS Manual Version 2 04 Oct 2004 Page 68 of 124 UVS SOFTWARE for UVIKON 900 XS XL SS AT USER MANUAL Rican Ware aie TUV iex E D Acquistion E Method Report E Stat E Abort x E 0 01988 0 00958 0 01437 0 01566 0 01497 0 01557 0 01084 0 01257 0 01317 0 01265 0 01617 0 00898 0 01145 0 01437 0 01737 0 01386 0 01018 0 00778 0 01566 0 01617 prreeeeeeeeeseeesseg Peak Detection Parameter Feseeesseseeesneeee Instrument Control Cell Changer Acquisition status Values Method frotet oo ea Autozero J Mode esar Sample HE Wavelength rim Abs tionfr Z _set Feed 180 550 0 0 3661 Samples E poston zi Sa Renan 0 min a 550 0 Set 0 0 21 06 45 Finished User admin Note If you have programmed more than one sample you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged If you repeat the measurement you will always measure the selected number of samples 1 in our example until you change the Samples parameter The system will prompt if the previously run sample data has not been saved before starting a new run AN Data not saved Continue anyway Click on OK to start the next acquisition run or on Cancel to return to the original data set Printing Click on the Print icon to obtain a
113. ure that the approval button is active All subsequent selections will be applied then to the approval process Acceptance The approval and acceptance areas are selectable using the check button immediately adjacent to the respective dialogues In order to accept a document ensure that the acceptance button is active All subsequent selections will be applied then to the acceptance process Login Name This is a drop down selection list which allows selection of the user who is executing the signature Only those users with the appropriate rights are displayed in the list i e for acceptance only users with acceptance rights will be displayed Select your user name from the list Full Name The full name is entered automatically once the user name has been selected as described above The full name is defined initially as part of the login definitions and cannot be changed here Password Entry of the user password is mandatory before any signature process can be successfully concluded Comment Here a comment relating to the signature can be entered and this will be stored with the other signature details and also shown on all printed reports Once a document has been signed off and accepted it is no longer possible to change that document therefore there are a series of prompts appearing to ensure that the user is aware of this process before actually proceeding The final prompt appears after the signee has confirmed that the document should b
114. verview Report Screen Click on the Report button in the upper left corner to open the Report window UVS Software for Manual Version 2 04 Oct 2004 Page 50 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL T arog TUV is EE eee zie PC Acquistion E Metrod Tb Repor Bci oe A HE Si 5 F kor Re Datt Uar ae Scan interval ed Import database x CO nmin Year 2002 The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item which can be manipulated or edited as required The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 2 3 2 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page 2 4 2 3 3 Importing a report from the database al Click on the mport icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened UVS Software for Manual Version 2 04 Oct 2004 Page 51 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Rishi oarea IU 2 4 2 3 4 Filtering the report list
115. warefor gt Manual Version2 04 Oct 2004 Page 37 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL ie TUV 2 4 1 1 4 System Status Wavelength nm Absorbance 0 0 0 0000 580 0 Set oo The lower right section of the display contains the actual status of the system and displays the following parameters gt Current sample being measured gt Actual wavelength gt Actual absorbance value gt Temperature where applicable n addition the lower right panel also allows manual setting of wavelength in order to allow rapid monitoring of the unit 2 4 1 2 Parameters in Fixed Wavelength Acquisition Clicking on the parameter tab in the acquisition allows access to the following parameters Factor 1 0000 Wie f Abs C T Factor The factor can be used to calculate concentrations or other values if the response of the substance measured is known In this case use of a calibration file would not be necessary View These checkboxes permit toggling between absorbance and transmission values for the display of the acquired data The data is always saved according to the definitions in the method independent of this setting UVS Software for Manual Version 2 04 Oct 2004 Page 38 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS a USER MANUAL Ricca n i UV 2 4 1 3 The Method in Fixed Wavelength Click on the Method button in the upper left corner to open th
116. wavelength measurement of that particular sample 2 4 5 1 The Acquisition Screen in Wavelength Program Select Wavelength Program from the main menu to obtain the following display PY wavelength program ale x Ia F Acquistion B Method E Repot E Fastet la Ly gj Ea le Darcie T T 7 nammen Cio Phoalomaiar Gunna Wavelenath nm 5600 sa pera T Sample 0 Wiwetenglh inj Abeorkance 350 0 0 0516 Samples a Biani m Samples 37 pe This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Number of blank samples measured as the first samples gt Autozero at start of measurement UVS Software for Manual Version 2 04 Oct 2004 Page 81 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Boe aioli arog ll UV 2 4 5 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel Method fo t s t SCi i s Ey Wavelength rim Samples 4 sao Set 5 Phetoneter Dariny Samples Defines the number of samples to be analysed Blank Samples Defines the number of blank samples to be measured blank samples ar
117. will display each data point collected on the curve as shown in the zoomed example below m Series 0 m Series 1 O Series 2 V m Series 3 M O Series 4 m Series 5 re a Ws Smoothing A filter can be introduced in the data acquisition in order to reduce noise Smoothing can be selected with as an averaging or a Bezier function High smoothing will reduce noise however high smoothing will also reduce the amplitude of the peaks obtained and can also result in distortion of peak shape Average This is a rolling average function based on 3 calculated points Only Y values are calculated P X X n 1 X ny X n 1 3 UVS Software for Manual Version 2 04 Oct 2004 Page 94 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL dT USER MANUAL Risto A TUV Bezier A Bezier curve is an approximation to a curve which would pass through all the control points The method used here for defining such a curve approximation is called the Bezier Bernstein approximation 2 5 1 4 Calculation The calculation function allows definition of mathematical functions which can be used to modify the curve data S is used to denote the sample itself and calculations are defined simply using S and the appropriate factor F and signs Addition Enter F S Subtraction Enter S F Multiplication Enter F S Division Enter S F For example it is also possible to subtract an offset from each point in the curve and then d
118. window Wiawarateassay SIE E e amp c A Dw ke Ha iadsa 0 09 02 2002 E oss ise U ei The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item which can be manipulated or edited as required The tabs for Activity and Peak Detection show a display in table form for the respective parameters The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 4 3 2 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 04 Oct 2004 Page 77 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL a in TTU 2 4 4 3 3 Importing a report from the database Click on the import icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened Import database X Tear 2002 Cancel Help 2 4 4 3 4 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user
119. with the actual date by which the password must be changed 2 2 4 1 8 Password This allows definition of the user password for normal access control and also for saving of data if the checkbox described is activated The initial password for the new user must be entered in the appropriate box New users will be forced to change this password when they log onto the system for the first time This is a precautionary measure to ensure that the system access password is known only to the user and no other third party 2 2 4 2 Groups This defines the grouping for report access Groups and members of groups can be defined to view the reports A member of a group can view the reports of all members User in group Lab group Miadity Workstation group UVS Software for Manual Version 2 04 Oct 2004 Page 21 of 124 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL SS dT USER MANUAL Pee 2 2 5 Baseline The UVS software does not automatically execute a baseline at start up This operation can be executed by clicking on the Baseline Run icon in the start up screen to open the following dialogue window ethiod Lamp change Lower a0 Wavelength miN Tungsten Lamp Deuterium Lar Upper 400 i Wavelength Fiter change 1 Fiter change 2 Fiter change 3 Fiter change 4 Speed l High speed Fiter change 5 Low speed OK Cancel The actual conditions under which a baseline is executed can impact later

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