Bioprocessing: Fermentors(New Brunswick), Description
Contents
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4. TO Ot _ 7p EH 00413 6 0418 70 nad 3483 6444 4 125 naan 125 ny Pump Tubi Chemical Compatibility es We assure o responsibility for the use of this Information in 4 ki affect changa Compatibilility Minor affect slight earrasian or a Moderate effect nat recommended fer continuces usa s ftening laas of strength swelling andor shrinkage D Severe elect not recommanded any use data All ratings in the chemical compatibility tables at right are at TOF 21 C Higher tamperatures will decrease the chemical resistance of tubing i rli mol specie applications Test aniy under specified conditions of your ewe H H Application tn ensure safe of chemical immersion tasting TE prederred for more accurate bast results H H ik mmarzian tesling grocedere Letra dz z HB i We recommend an immersion test to chack compatibility Always preteen vifi unten or marginal chemicals immarse a sample rhis i H langth of tubing at least 48 Fours in closed vessel pontain Kuren cuf Bioprocessing ing the fluid to ba pumped Examine the tubing tor saling ad iis B erribrittleeeni cracking saf
5. 3 10 Hl27i 9242 t 10 1169 6000 2 20201 04958 L 3 740 2501 STLCC CPLS Morrison 4 11 2014 Description CONDEHSER ASSEMBLY 0 91 ZL CAP D 9L 2L 5L CORE WELDMEHT CERTI PIED CAP TUBE WE LIJMEN T 0 9L 2L GASKET FILTER ABSOLUT E l 4 1 2 O0LD TUBING SLOW LB ID D EA EXHAUST CONDENSER ASSEMBLY 1 4 1 7 5L BF115 UOM 1 000 Active 1 000 Active 1 000 Activa 1 000 Active 1 000 Active 1 000 Activa 0 170 Active Engineering Ball Havision Bete zZ2Ul2z 01 1 09 48 Rev Description Item Category 02 04 085 04 080 09 04 9350 02 04 080 09 02 0561 g 02 080 01 02 055 26 Bioprocessing Exhaust Condenser and Solenoid Valve Connections MOUNTING VALVE SPLITTER PLUG connect BRACKET to rear panel of Power Controller A see Figure 2 HOSE BARE 2 connectto cooling N coll orwater Jacket 3 HOSE 1 connect to water source downstream of pressure regulator VALVE BODY STEL FILTER WATER OUTLET WATER INLET epee ini port adapter EXHAUST PORT DFLA TE STLC Page 27 Bioprocessing Sampler System SAMPLER PORT STRINGE FILTER MPLER BOTTLE HOLDER SOMPLER BOTTLE MA HESOPLATE CLaM PING HEADPLATE CLAMPING SCREW
6. de SAMPLER TUBE STLCC CPLS Morrison 4 11 2014 Page 28 Bioprocessing 1 3L Sampler Harvesting THUMB CLAMP STRINGE FILTER STRINGE 1 8 SILICONE TUBING REDUCING ELBOW 316 tubing on Sampler holder SAMPLER P um HARVEST 6 PORT 22 SAMPLER BOTTLE HEADPLATE HOLDER SPARE SAMPLER BOTTLE q HEADPLATE CLAMPING NUT HE ADPLATE CLAMPING SCREW SAMPLE TUBE HARVEST TUBE STLCC CPLS Morrison 4 11 2014 Page 29 Bioprocessing Foam Trap SILICONE TUBING STERILE FILTER N FOAM TRAP BOTTLE 250 500 ml FOAM ESHA LIST TUBE HEADPLATE BOTTLE HOLDER BOTTLE HOLDER MOUNTING ARM HEADPLATE CLAMPING NUT VESSEL STLCC CPLS Morrison 4 11 2014 Page 30 Bioprocessing BioFlo115 Sparge Connections Figure 1h Sparge Connection detail from Figure 14 Connect the barbed sparge connector here STLCC CPLS Morrison 4 11 2014 Page 31 Bioprocessing Lab Air Bench Valve and Regulator STLCC CPLS Morrison 4 11 2014 Turn black axial valve Locate AIR valve and regulator behind the NB controller 8 stack closest to whiteboard Make sure main chrome valve is open line position behind gage clockwise to open and increase pressure to desired level 10 15 psi Rear of Controller 8 stack Page 32 Bioprocessing Autoclaving Objectives and Methods
7. 4 Air Shower HEPA loose contaminants Change Room ern mesa 5 Hand washing station no touch operation 6 Hand dryer HEPA filtered air no touch E eo s 7 Dispenser for glove liner or underglove p O 8 9 Glove washing drying station 10 Headwear dispenser Station 6 11 Face mask dispenser Fane Garment 12 Waste receptacle check 13 Garment Storage Facility Uleanroom ag 13a Open Rack for reusable garments Bio Cleanroom Air Lock 13b Enclosed Cabinet for reusable garmen Aseptic Area 13c Shelves for disposable garments ee UU 14 Waste Receptacle 15 Ergonomic Lean Rails support personnel while donning 16 Gowning Platform provides noncontaminated surface to prevent violation of garment 17 Wiper dispenser used to clean bench prior to putting on booties 18 Bootie shoe cover dispenser may also be incorporated into benches 19 Waste Receptacle 20 Gowning benches used when putting on booties Booties stay on clean side 21 Cleanroom mirror for final self inspection side by side w poster showing correctly garbed model 22 Safety glass and overglove dispensers 23 Air shower may be required in cleanroom entrance 24 Garment hamper for reusable garments to be laundered 25 Waste receptacle for disposable garments 26 Tools supplies cabinet for overstock garment
8. 61 770 Fi EE 293 293 117 6_ Total Parts 10 13 15 16 Bioprocessing BioFlo115 Accessories Parts 2 2 Page 12 Bioprocessing Fermentor New Brunswich BioFlo 110 BRDG Multi Language Primary Control Unit controls and displays up to 32 process parameters Simultaneousty handles up to four vessels Levels Foam Controller regulates antifoam as well as media addition remowval Rotameter for manual gas flow control pH dOs5 Controller regulates a pH probe and a DO probe Power Controller regulates temperature agitation and up to five pumps Pump Module has four independent pumps for acid base antifoam liquid addition removal One module may be shared by multiple vessels STLCC CPLS Morrison 4 11 2014 Direct Drive Condenser Sterile Sampler Autoclavable Vessel Stand is provided with heat blanketed vessels Heat Blanket with two viewing windows provides precise temperature control single walled vessels Page 13 Bioprocessing Fermentor BioFlo110 Manuals amp Information Hot Link to New Brunswick BioFlo 110 Operations Manual pdf 3mb Hot Link to Overview Pichia processing pdf Hot Link to Paper on Pichia Protocol pdf Hot Link to Paper on Anaerobic Yeast Ethanol pdf Hot Link to New Brunswick BioFlo115 Brochure pdf Hot Link to New Brunswick BioFlo115 Brochure Parts and Desc pdf STLCC CPLS Morrison 4 11 2014
9. Crossflow Satorius Stedim BRDG New Brunswick New Brunswick Fermentor Fermentor BioFlo 110 x 3 Units BioFlo 115 x 2 Units BRDG BRDG Bioprocessing Systems Detect Instrument Picture to Jump to that section of SOP Tubing Info ThermoFex Chiller for Bio Vessels Prepared by Bob Morrison SILCC Instrumentation Specialist Original Oct 08 Latest revision Feb 2013 Gas Tanks amp Supplier Karl Fischer titration STLCC CPLS Morrison 4 11 2014 Page 1 Bioprocessing BioFlow115 New Brunswick BioFlo 115 PN M1369 5111 B115BG400336 2 3 12 RGM Received to R119 Registered NBSC website 2 9 12 BioFlo 115 PN M1369 5120 B115BG800337 2 3 12 RGM Received to R119 Registered NBSC website 2 9 12 With 1 3L and 14 0L Vessels Hot Link to New Brunswick BioFlo 115 Operations Manual pdf Hot Link to New Brunswick BioFlo115 Brochure pdf Hot Link to New Brunswick BioFlo115 Brochure Parts and Desc pdf Grow virtually any cell type aerobic and anaerobic microbes yeast insect plant and mammalian cells Water jacketed and heat blanketed autoclavable glass vessels are offered in four sizes 1 3 3 0 7 5 and 14 0 Liters total volume All 115 systems are pre programmed with both fermentation and cell culture operating modes for total flexibility Switching between modes automatically adjusts gas flow and speed ranges A wide variety of options and accessories provide the versatility for customizat
10. To minimize pressure differences throughout the stenlization process by ensuring that the air can transter freely between the inside and the outside of the vessel B Te ensure that minor pressure differences do not expel liquidtrom the vessel by clamping off all penetrations that go below liquid level C loprotecthydrophobic filters trom blockage which would occur if condensation were allowed to wet and block the filter surface D Te protect susceptible vessel assembly components trom steam damage The first objective 15 met by leaving at least one vessel port open the second by clamping shut flexible tubing attached to immersed penetrations and the third by wrapping filters with a protective cap of aluminum foil Use protective caps on probes and bearings to meet the fourth objective STLCC CPLS Morrison 4 11 2014 Page 33 Remove the motor trom the top of the vessel and carefully put it aside 2 Lubricate the vinyl bearing housing cap with silicone grease to facilitate sliding the cap securely onto the housing 5 Place the bearing housing cap on the top of the bearing housing 4 Discennect the ar and or gas lines from the inlet filter on the sparger Bioprocessing Disconnect the water lines Remove all PVC tubing TP 6 Clamp off the harvest tube the sample tube and all other penetrations that are Autoclave Initial immersedin the media Steps with d Remove the RTD trom the thermowell 5 Disconnect all probes and se
11. pa tapaq ant arc 4 JT 1OY uit ur pact ferr 106405 Cat wets FDA approval tor food rainy aps cat orn Longest Me good low consistency arf Hes aj ree gant 1 59 Good resistance ta es no Heat serija ard fielecric parsiani PLU acms Sm VT ATI LES est resisant nubis Resim CNTOSWES solvents 991 oiis of ferpens Line 94 Www 167 36 recorrrecced Fam manera uuu EM VERTS PRET EIL Val tram nui est Emelt Cerca Martens fid perty w aat steers eat Hoiek tanaty ug te 990 psi easy mastering Very kra OM Smooch bore US Class VI Page 41 Bioprocessing Gases Flashback Arrestor Airgas supplier From Airgas STL PN Y33 1FA44 gt Manf SuperFlash Model DGN Fuel Flashback Arrestor for Regulators and Point of Supply Model DGN flashback arrestors can easily be installed on the outlet fitting of most regulators and gas supply points The
12. such as phosphate balanced saline solution sterilize the media separately after autoclaving the vessel 1 I you have vessel assembly that 1s too tall for your autoclave carefully lay the vessel still mountedinits stan dif present 1n the optional angled autoclave rack pari number AM B S023 M 1 22 7 08 Figure 37 below Secure itin place with the strap 2 lfthe vessel 1s not water jacketed skip to Step 3 If the vessel is water jackete d the jacket should be halt full tor autoclaving see 4 5 3 jor Aline jacked Make sure that the Water In line connected to the bottom of the jacketed vessel is pinched closed to avoid water leaking trom the jacket during autoclaving 5 Insert the entire vessel assembly glass jar vessel standit present headplate and all headplate components inte an autoclave and sterilize 4 When you remove the vessel trom the autoclave immediately cnomp the foil funnel on the addition port and close off the vent tubing to maintain sterility STLC age 35 Bioprocessing Vessel Autoclaving Liquids Cycle 253C Wrap all filter ends with aluminum foil to protect from steam and condensation Add 1 2L liquid to cover pH and DO probes and orient vessel on cradle to cover the ends of the probes Make sure the vessel is vented to avoid overpressure and potential cracking of the vesel STLCC CPLS Morrison 4 11 2014 Page 36 Biopro
13. Hone pre assigned 90 om available loops wvill change depending on _ Nos configuration This one is equipped with p pL Loo SCREEN access gt ER Cascade Pumps buttons Lg _ amp NOTE The dark blue button usually represents the screen being displayed STLCC CPLS Morrison 4 11 2014 Page 5 Bioprocessing BioFlo115 Ex Cell culture without Thermal mass flow control Figure 19c Sample SUMMARY Screen Cell Culture without Bie x UNIT TAB OPERATING IC ON _ Summary Screen _ MewEBrunswick Cal Cuitwe Mode MODE 07 Dec 2008 15 32 Your gt ss owe ve amp TME pre assigned 1593 1400 1000 Auto pH loop names The your system s 44 sa 44 faca rh oo oo oo Gas Mix _ 200 200 462 ff SCREEN ACCESS buttons Calibration B Cascade Lx Setup gt NOTE The dark blue button usually represents the screen being displayed STLCC CPLS Morrison 4 11 2014 Page 6 Bioprocessing BioFlo 115 Notes on Calibrating pH Probe lan R 8 1 12 e Note pH probe should always be stored wet never allow to dry Do not store in DI water Recommend 7 0 buffer e Go to BioFlo Calibration screen from bottom of main menu Rinse probe with DI put in 7 0 solution wait for Raw Da
14. Palvsulfane Feed Vessel 3161 1 4404 1 4435 55 5 Slice 200 Tank Volume 500 ml Holder Min Operating Volume 20 ml Sartorius Laboratory Balance Talent TE 4100 Medical Grade Pressure Transmitters 900 RPM Integral Stir Plate and stir bar Connections Luer Luer Fittings T pieces Valves and Ven tz ONT REC REO PCR Finch Valve for back pressure control 6 feet af Phar Med 15 Tubing Balance and Pump 5232 Interface cables System Pressure 58 psi 4 bar Dimensions VV x D 50 em x40 cm H5232 Camrmumnicatians Exchange Win Wedgel SartoWedge PC Software Alr Filter X Butt er Retentate Alr In lat Return Valve el he Ug age 50 Bioprocessing Filtration Karl Fischer Donated by Denise Heinz mailto denise heinz apertuspharma com titration method in analytical chemistry that uses coulometric or volumetric titration to determine trace amounts of water a sample Bioprocessing Filtration Vacuum Examples Polypropylene Porcelain Hirsch funnel Hirsch funnel 50 mL Filter flask STLCC CPLS Morrison 4 11 2014 Page 52 Biosafety Clean Bio Safe Gown ning Room Design Features HH m 9 99 9 gio start 1 Secure lockers for personal property c Zone 2 Shoe cleaner w brushes 7 Here 3 Walk off adhesive shoe mat ee Head Face E
15. S AMPLE RSP ARE ASSEMBLY TRI PORT SEPTUM ATO THERMIOWELL see NOTE below Each is a possble mount ha position for a battle holder STLCC CPLS Morrison 4 11 2014 Page 18 Bioprocessing Rotameter on Air Supply Figure 34 Rotameter Side View AIR OUTLET BARE Connect to sterile inlet filter on wessel head plate L J FRONT GAUGE HANGING BRACKET EN AIRFLOW ADJUSTMENT AIR INLET BARB KN B Connect to clean air 4 Source 3 Attach another piece of silicone tubing to the top air outlet hose barb see Figure 24 above Insert the other end of this tubing into the inlet stenle filter on the top of the vessel headplate 4 Verify that the exhaust port is connected to a filter If you are using the optional exhaust condenser the filter should be attached to its outlet 5 Fead the the top flat edge of the float To increase airflow tum the adjusting knob counterclockwise To decrease airflow turn it clockwise flow rate Fermentation 11 4 Cell Culture 2 5 STLCC CPLS Morrison 4 11 2014 Page 19 Bioprocessing Probes and Tubes Temp probe Solid Harvest or Innoculation Sample Tube Tube Sparger with small holes STLCC CPLS Morrison 4 11 2014 Page 20 Bioprocessing dO2 and pH Probe Assembly Fill with squeeze bottle solution if required Note some models are permanently gel filled 402 PROBE ADA
16. the culture L Trials every 3 minutes and can maintain a high level of process control People on the move jobs in pharma Excipact scheme to launch next week 2 People on the move jobs in pharma West continued by saying that Finesse and Nova will be showcasing a new 30 e WJ 30 note gadget EB Ol Inbox Mail on tine gluc P Bioprocessing 2 4 On line glucos old 8 46 OILUUV UI 4 1 1 cU14 60 Bioprocessing Gas Oxygen OXUSP200 from Airgas All Categories Gases Medical Gases Oxygen Medical Oxygen USP Grade Size 200 Cylinder CGA540 x High HM OE B B 138 Enlarge Image PART NUMBER USP200 UNIT PRICE Reg Price Call 855 440 8095 for price and availability Web Price Call 855 440 8085 for price and availability Min Purchase Qty 1 CL PRIF STLCC CPLS Morrison 4 11 2014 Page 61
17. 2 Google P 1 www in pharmabechnalogist com Processing OC On line glucose measuring Wwith Finesse File Edit View Favorites Tools Help 5 Favorites 55 4 YSI Life Sciences 2700 Sensor Solutions Fines m On line glucose yy Home Feeds J Read Print Page Safety gt gt E E STLCC Computer Support ds Center For Plant and Life Sci STLCC FV SMET Division Dell Education Purchase Pro gt St Louis Community College Ed Lifecycle management On line glucose measuring with Counterfeiting Fi inesse By Dr Matt Wilkinson 05 Apr 2007 Mergers and acquisitions Related topics Processing amp QC WHEN YOU NEED TO BE SURE The first step towards true in line glucose monitoring for cell culture bioprocesses has been made that will allow greater control over the bioprocess manufacturing In collaboration with Nova Biomedical Finesse Solutions has combined Nova s Bioprofile 400 mammalian cell culture analyser with an online sampler and feedback controllers A The company claims this will delwer automated on line round the clock glucose monitoring and feed control for cell culture bioprocesses to a level of control not previously attainable MOST POPULAR NEWS Glucose monitoring and control in bioprocess manufacturing is needed to ensure the opti
18. 3 3 0L Maine Blade 75 amp 140 L Alter Suspension Cells 1 3 L Alter Suspension Cells 3 0L Alter Suspension Calls 7 5 L Alter Suspension Calls 140 L Alter hierecarrierz 1 3 L Alter fwicrocarriers 3 0 L Alter fiwicrocarriers 72 L Alter fiicrocarriers 14 0 L Catalog Mo Description Price Heplacement Exhaust Conde neers ul 1273 2 E For 1 5 3 0 and 7 5 vessels hile 3 2 For 14 0 L Vessels 890 Headp hte Adapters amp Plugs 1273 9997 Tri port Adapter 247 1273 01 Probe Adapte SE 12 mm compression 7273 5042 Foam Level Probe Adapter dE 12mm compression hil 1273 3051 Septum Fit 225 3 4 Emm Adapter kit 150 Lemm port tor emm tbe lul1273 2e75 Emm Single Addition Tube 7 for emm adapter 3 Adapte Kit 123 12mm port to emm tube 7275 8085 Adapte Kit 143 12mm port to 12rrrn tube li1273 340B 6 35 Port Hug hi1273 3405 12mm Port Plug ul 127 3 AAT 18mm Port Aug Sb Replacement and Spare Parts 1273 501 Hesdplate Port Washer sd Rings 75 Iul 12773 50509 Parts Kit 13 amp 3 0 L Vessel db Spare Parts Fit 75 amp 140 LHE 492 477 25b 2085 2210 2 340 2465 2055 2210 2 340 2 465 Catalog Mo Description Micros 273 5007 Sintered 5 hiereeparger 13 Wessel 273 5005 Sinte
19. E dd ARE DATE 3 3 00 3 22 00 DRAWING MUM BER 1275 5040 DATE 1 Probe Adapter 12 mm compression Effective Rey Quantity i Description Time ADAPTER 1 000 12 JAN 07 14 52 COMPRESS ION 12mm XNURLED NUT G EA 1 000 12 JAH 07 14 52 En FERONT EA 1 000 12 BN 07 bz TEFLON 12mm RULE BACK T B 1 000 12 JAN 07 14 52 EFLOM 15mm O RING 3 013 F EA 1 000 12 JAN 07 14 52 WASHER 0S0 TH A 1 000 12 JAN 07 14 52 505 ID TEFL End of Report n Engineering Bill Mo Revision G Date 2011 08 02 13 34 01 1 Date Time Extended Qty Eco 1 090 000 000 000 000 1 000 H Page 24 LEMME EMEN FUTE AMD EXHAUST TUBE ar 14 4 USE MOT NMCLUDED THS Ede ON RETURN ums UNE 94 USED TO WATER CUSTOMER TO amp ucUMT CF Gur CF COMDERSER Bloprocessing Exhaust Condenser Details 9945 M1369 ir N 3 LG SEE 25 5 Bioprocessing Exhaust Condenser Bill of Materials Organization Enfield Manufacturing Item 1369 9945 Alternate Primary Bill Of Material Commenta Comment Text Component Item Laval Seq eq Iter 1 1 1 121 3034 1 19 1213 9434 2 10 1 169 5 040
20. PTER Calibration top portion Ph 7 Set Zero wait until counts FERPLILE Stabilize then enter value then BOTTOM FERRULE m return key 402 PROBE ADAPTER bottom por tian X to get back to next setting w TEFLON O ING white Ph PORT O RING black GAP pH PROBE ADAPTER n tap por tion Bottom Fa TOP FERRULE Pe pH PROBE ADAPTER 4 ttam par tion TEFLON ORING white PORT O RING Black STLCC CPLS Morrison 4 11 2014 A oo oa a a i mamam Q sl isma a re m a Ne Ae WE M j 8 7 enfichable 2014 10 When unpacking check electrode for possibla mechanical damage to the membrane 1 the diaphragm 3 or the body 2 Electrodes sut ject to complaints must be returned to us or to our agant in the original box indicating the invoice number off watering cap 7 and flush electrode with distilled water After rinsing with dist water wipe the elec trade with tissue Do not rub as an increase of the response time might result Remove rubber cap which covers the elec trolyte filling aperture 4 or out off nipple T to ensure pressure equalization with the atmos phare Eliminate any alr bubbles inside the membrane space 6 of the measuring electrode by smo
21. Page 14 UHIT 2 ADDITION CONTROL BOTTLE UHIT 1 MODULE 5 mounted on COH TROL MODULES Wessel ase UNIT 2 VESSEL In Vessel Stand closer look p PCU wath display screen ak and touchpad a Gas Mix Module Level Control Module 2 Control Module Power Controller wath Rotameter STLCC CPLS Morrison 4 11 2014 Page 15 Bioprocessing Fermentors Bioflo 110 Vessel Components AGITATION a MOTOR USING EXHAUST B HEADPLATE LIFTING 1 HANDLE 177 CLAMPING 4 LF RING BAFFLE COOLING COIL hides 5 COOLING COIL THERM O WELL VESSEL STA ND SPARGER STLCC CPLS Morrison 4 11 2014 Page 16 Bioprocessing Fermentor 7 5 and 14 L Headplate Definition COOLING COIL SPARGER COOLING COIL x PROBE HARVEST 4 7 SAMPLER TUBE H UST MOUNTING POSITION FOR SAMPLER ASSEMBLY dO 2 PROBE PLUG 6 35mm SPARE LFT Mi ATO C TRI PORT TRI PO SFP TI H PLUG 1 PLUG 12mm SPARE Each is a possible mounting LEVEL csition for a bottle Paler STLCC CPLS P Page 17 Bioprocessing 1 3L Headplate configuration SPARGER COIL LEVEL PROBE TRI PORT 102 PROBE HARVEST
22. age 22 Bioprocessing DO Probe Kit 1 3L Adapter Assembly Details Organization Edisen Manufacturin ltem 1369 9974 f 115 DO Electrode Kit Mettler Ingold 1 3L Engineering Bill Alternate Primary DOM EA R vision Dale 2011 08 05 10 14 Components Item Op 2 Effectiva 1 Disable Level Seq Seq Item Description Rev Quantity Date Time Date Time Extended Qty ECO l 1 10 20720 6580 BLECTRODE DO 1 C 1 000 15 JAN 09 12 53 1 000 1 2 10 M1273 5040 Probe Adapter G EA 1 000 15 09 12 53 1 000 1 3 10 1273 9147 PROBE SLE H EA 1 000 15 09 12 53 1 000 1 10 20720 72336 CABLE DO D 1 000 15 09 12 53 1 000 1 5 1 0 20640 90465 BAG PLYE 004 EA 1 000 15 09 12 53 1 000 1 10 1369 8610 LABEL 1 3L IN A 1 000 15 09 12 53 1 000 End of Report w Page 23 STLCC CPLS Morrison 4 11 2014 PLAC road 5352 280 6592 WITH 0956 Organization Edison Manufacturirm Item M1273 5040 Alternate Pini Components Item Op Level ceq Seq Item 1 1 10 M1273 9400 1 2 10 1273 9580 1 3 10 PO2430 3200 1 4 19 PO240 3201 1 5 10 2280 5352 1 19 P0100 9870 ST Bioprocessing Headplate Adapter Adjustable 12mm required for pH DO Condenser METRIC TOLERANCE K t 4mm KK z lomm DA HG REF AD USTABLE ADAPTER UNLESS OTHERWISE SPECIFIED NENT ALTER AR
23. andard disposable reactors such as spinner flasks roller bottles T flasks hyper flasks Erlenmeyer flasks cell stacks customer specific systems bag reactors and other SUBs single use bioreactors The sensor is fit in the corresponding original cap subsequently gamma sterilized and delivered to the customer ready for use o 158 The ClTSens Bio beamer is a radio transmitter which transmits the measured data from the CITSens Bio sensor glucose lactate or glutamate to the ZOMOFI CITSens Bio ZOMOFI receives data from ClTSens beamer which have been collected online by either ClTSens Bio sensor glucose lactate and or glutamate ZOMOFI can receive measurement data from up to 1000 beamers and transfers them to the PC by means of RS232 or LAN The ClTSens Bio software imports the data and stores them in a database The measured values can be processed displayed and saved by the means of the ClTSens Bio software g l und nA STLCC CPLS Morrison 4 11 2014 Page 58 Bioprocessing Info YSI Glucose Monitoring control On Line Analysis For processes that require frequent monitoring and regulation on line analysis allows you the freedom to sample automatically and adjust both nutrient and byproduct concentrations Couple the YSI 2730 Aseptic Monitoring amp Control system with the 2700 SELECT and pull samples directly from a bioreactor measure important nutrients and byproducts and immediately control the r
24. cessing Service Repair Training Info pH Probe Email to Mettler Toledo for Quote on pH probe 405 DPAS SC K8S 325 mtprous mt com Other Probes or New Brunswick items Get Part number from Manual send to Teri Hacker Chiller Thermo Sci Get quote from Fisher Scientific FISHER SCIENTIFIC COMPANY LLC 4500 TURNBERRY DRIVE HANOVER PAR KIL GO 135 549 4 Bioprocessing Training Nov 10 to BS BM by Toby Mapes toby mapes gmail com Tubing Order Info PO to Cole Palmer per TH 1 20 11 Item Commodity Description 1 EW 06422 05 Clear C Flex tubing 1 4 OD 2 FF 98906 04 Tubing Pt cured silicone 1 4710 300 3 EW D6508 16 Masterflex Pharmed BPT tubing 16 4 sg amp h STLCC CPLS Morrison 4 11 2014 Page 37 Bioprocessing Tubing MasterFlex Sizes Masrenflex 116 PRECISION PUMP TUBING HIGH PERFORMANCE PUMP TUBING 01 91481 841 4 nap wt 6 to H8 tree TLLA 14 hie H 0 uy m 17 AT MH ran Hg 298 M30 us Dung Baty wn mw LS perp head ai are LIS E te 10 gm dee capable of rura pg two ot heida 05583 16 15 isting Flow segs 09 0 Murren corbeasus 100 EA kart 23 1 7 Brel peq pack R 06585 1 173 ISHP Flow ange 1 7 770 riin Midten EO 5 5 bar 2 n 7 0 out LD PTFE Tubing Sets kk wwe 157
25. cordingly have nai boen ar revered approval from fh F U devices dar medical usa Page 39 Bioprocessing Tubing Features Contraints pg 1 2 Appiscalips suitability Eecefent biocompes bi ty Me addas ar plastictrers phthalate and and rertrer hiye nisat Ma taste most 0 10065 Extra gaat over a wide lerspergum ange Weather tor coni ane tadaboe esta tereescy to gue 2 set AG01 Foor CAME SOEVENTE far VACIMM 7055 Pune Ffar FUIT Silicone pinum ured 96410 US Ces Can wit 5 or hol ahd sei Le FDA 21 CFR DOP or 900055 anc sociae c MANES Use soap Such as leary Do ret sse or p USHAMIU SOLVENTE We be Kous 12 transporter PRLESUGE tar by ad good om beige ras ren fe that Weaver crore corona natation deel Bed vole Mim y tendency set ETO Rug Sm dat on Aarclirabke
26. dependent alarms allow the user to stop the crossflow process automatically or to give an alarm e g after a predefined permeate weight signal is triggered or a maximum pressure limit is reached All values are transferred to a Worksheet of the Sartowedge data collection software Some visual graphs are predefined for fast process control User defineable alarms for reliable operations eOn board application programs eFinger tip control via the front panel elnterfaces directly to PC FEATURES eOptically encoded and servo controlled pump motor eReal time process verification and documentation eTandem 1082 Peristaltic Pump e500 ml Graduated PES Polyethersulfone Feed Vessel e316L 1 4404 1 4435 SS Sartocon Slice 200 Holder eSartorius Laboratory Balance Talent TE 4100 eMedical Grade Pressure Transmitters e900 RPM Integral Stir Plate and stir bar eLuer Fittings T pieces Valves and Vents Hot Link to Satorius Slice Brochure parts list pdf ePinch Valve for back pressure control feet of Phar Med 15 Tubing and Pump 5232 Interface cables Printed manual available in Bioprocessing room eWin Wedge SartoWedge PC Software No online digital manual has been located STLCC CPLS Morrison 4 11 2014 Page 49 Bioprocessing Crossflow Slice Diagram Technical Specifications Output 0 2 200 ml min Major Components amp 40 psi 2 5 bar Tandem 1082 Peristaltic Pump 500 ml Graduated
27. eplenishment of nutrients if needed while maintaining the sterility of the process All system components are sterilizable How On Line Monitoring Works The YSI 2730 Monitoring and Control system pulls sample directly from a bioreactor and pumps it to the YSI 2700 SELECT at programmed intervals After each analysis an antiseptic reagent typically a dilute hypochlorite or hydroxide solution is flushed through the sample lines and remains there until the next cycle to assure sterility For those bioprocesses in which loss of cells is a concern you can connect the YSI system to an on line filtration device You can adjust system parameters such as flow rate to accommodate the needs of the filtration device For regulation connect the YSI 2700 SELECT to a feed pump When nutrient concentration falls below a programmed setpoint the YSI 2700 SELECT will signal the feed pump to add nutrient to the bioreactor Other system variables such as sampling interval and concentration setpoint allow you to customize the instrument for your process needs For example with processes that consume glucose quickly you can configure the system to monitor glucose every few minutes For other processes that consume nutrients slowly monitoring every 30 or 60 minutes may be more appropriate STLCC CPLS Morrison 4 11 2014 Page 59 Bioprocessing Info Online Glucose with Finesse 2 On line glucose measuring with Finesse Windows Internet Explorer E x 2 X
28. ia luerlock port on Sampling tube discard this media as it would be in the extraction pipeline and possibly not all from vessel volume Using a fresh sterile syringe extract desired amount of media Test in Spectrophotometer for optical density STLCC CPLS Morrison 4 11 2014 Page 10 Bioprocessing BioFlo115 Accessories Parts 1 2 Catalog Description Price Catalog Ma Description Price pH Probe Kits Probe Cable Adapter Replacement Motors 1 6 For 1 3 L Vessels 794 1138 3120 Direct Drive 1 015 11362 20977 For 3 0L Vessels 800 1 3 amp 3 0 L Rerrrentors 13869 2882 hor 75 L Vessels 824 1358 31 265 Direct Drive 1 070 1369 2585 For 14 0 L Vessels 934 For 75 amp 14 0 Replacement pH and DO Probe Cablet4dapter vl 1389 3135 Direct Drive 1 015 PoT20 2276 pH cable fall vessels 407 All vessel sizes Cell Culture Po 720 2336 cable tall vessels 5d4 1363 3130 Magnetic Drive 1 120 41273 50440 probe achpter For All vessel sizes 12mrn compression Replacement Heater Blankets DO Probe Kits Probe Cable Adapter 11365 65021 For 1 3 L Vessels 355 11362 29 For 1 3 L Vessels 2 770 11362 59022 For 3 0 L Vessels 355 369 9979 For 3 0 Wessels 2 825 vl 13689 5020 For 75 Vessels 381 1 369 9356 For 75 L Vessels 2 345 1388 8023 For 14 0 L Vessels 381 138 3558 For 14 0 L Vessels 3 070 Replacement Water Jacket Heaters Replacement Foam Level Kits Foam
29. ion to your exact needs Choose from eight vessels three motor drives specialized impellers up to four Rotameters with multiple gas flow ranges a Thermal Mass Flow Controller TMFC STLCC CPLS Morrison 4 11 2014 and more Page 2 Bioprocessing BioFlow 115 Gas Connections Insert the tubing into the connection simply by pushing itin Check to be sure the connection secure by pulling gently on the tubing If the controller equipped with an automatic gas mixing module set up your gas supply this way Gas 1 Air Gas 2 Gas 3 Gas 4 CO STLCC CPLS Morrison 4 11 2014 Page 3 Bioprocessing BioFlo115 Cabinet Controls Touchscreen CONTROL CABINET ROTAMETER S You may have from Oto 4 rotameters The quantity of and flow rates for the rotameters will be determined by your system specification STLCC CPLS Morrison 4 11 2014 Figure 18 Touchscreen TOUCHSCREEN DISPLAY Only the control station equipped with a touchscreen utility stations do not have one PUMPS ON OFF POWER SWITCH Page 4 Bioprocessing BioFlo115 Screen Summary ex Figure 19a Sample SUMMARY Screen Fermentation with Auto Gas Mix SCREEN UNIT TABS OPERA TING and MA EM Summer Screen New Brunswick Fermentation Mode IC ON OF Dec 2008 15 21 Loophamae Mode Units Current DATE Your BioFlo CelliGen om Dec None 115 comes with
30. mum conditions for growing cell cultures that produce complex 1 EU could make API suppliers verify starting therapeutic proteins such as monoclonal antibodies material sources Biomanufacturers have been forced to use manual methods of sample 2 US hits Ranbaxy with injunction to boost extraction and analysis to measure the glucose levels according to Finesse manufacturing quality This data would then be manually fed into the bioprocess controller which would g quay add more glucose as the cell cultures required 3 Novartis hit with another 483 after FDA Larry West executive vice president of sales and ma rketi ng at Finesse believes finds repeat failings RECON 4 Pfizer recalls birth control pills after blister packaging error While in line and in situ sensors for pH and dissolved oxygen are available the J D scover implantation of an in situ glucose monitoring system has proved difficult 3 to cut 1 350 operations support jobs Ca DSL Any glucose sensor would need to be able to withstand the harsh sterilisation techniques used to clean bioreactors that often employ aggressive chemical All In One cleaning methodologies or heating the reactor to temperatures over 130 C for Solution over 30 minutes AUDIO amp VIDEO NEWS TRAP According to West the latest offering is the closest thing to real time in situ for CI OF bioprocess glucose control achieved to date The system will sample
31. nsors and remove their cables Hardware ft you are using pH and DG probes install each probe s shorting cap providedin the probe kit 10 Before placing the vessel inte the autoclave loosen the glass sample bottle by 15 turn 11 Wrap all filters with aluminum teil to protect them from steam 12 Attach a piece of tubing wrapped with some non absorbent material such as glass wool or non absorbent cotton to each of the addition ports Wrap foil around the end of the tubing shaped like a funnel to allow the vessel to vent more easily during autoclaving Place a clamp on the tubing NOTE Be sure to leave one clamp open during autoclaving to equalize pressure If you have addition foam trap or harvest bottles mounted at the base of the vessel you can autoclave them with the vessel Without detaching their tubing from the headplate 15 Remove the bottle holder s and reinstall each on one of the headplate clamping SCIE WS 14 the bottle and turn the holder until the bottle and holder are positioned over the headplate rather than extended over the edge 15 Finger tighten the knurled nut Page 34 16 Clamp off the tubing and where appropriate remove it from the pump Bioprocessing Autoclaving Media Notes you will be doing batch fermentation be sure the vessel is filled with media so the media will also be sterilized ryou will be using heat liable media use at least 100 ml of a balanced salt solution
32. nt line Added Mar 11 to countertop Vertical line PVC to Ceiling a Exhaust vent EY 18 EA Hotlink to Wexford BRDG Park Drawing New Work 1 floor M11 201 SILCC CPLS Morrison 4 11 2014 Bioprocess Motor Power Consumption Access for Amp Meter Mike Davies project July 2011 Access to power consumption of motor agitator Located positive current line in control cable per manual diagram cut for access to Amp meter STLCC CPLS Morrison 4 11 2014 Page 45 Bioprocessing Chiller ThermoFlex 900 Neslab Thermo Specifications Reservoir Volume 1 9 gal 7 2L Temperature Range 5 to 40 C Ambient 10 to 40 C Temperature Stability 0 1 C Display LCD Order Bags from Fisher Scientific per info below 01101010000006 900w Pumping option PD1 115V 1 1 EA HL 9596069 FLUID BAG FILTER 2283 22 83 Vendor Catalog 0937 77 Original Catalog Humber 0937 77 1 On Off toggle at top rear 2 Push On Off 3 Set Temperature Hotlink to NesLab Thermo Chiller User Manual pdf STLCC CPLS Morrison 4 11 2014 Page 46 Bioprocessing Chiller Set Temperature Programming Setpoint Loop 5 STLCC CPLS Morrison 4 11 2C B Ensure the controller is either a blank screen not vet inutiali ed of displaying the process fluid temperature B Press the key and the controller display wall alternate between SP and the setpoint value lino change 15 required press the key to ret
33. ock connector and an inline Y adapter Halkey Roberts Corporation An Atrion Company 11600 9th St N St Petersburg FL 33716 Tel 727 577 1300 Fax 727 578 0450 8 245600xxx Series SWABABLE Y VALVES Bob 12 10 2010 Here is the bioprocessing ideas that Dennis Cooley threw out at me last Wednesday I m just passing on his ideas valid or not for future use It looks like the filter off the condenser was somehow getting clogged and was not passing air afterward His suggestion was to run tubing from the condenser to a catch vessel and then use a filter on the catch vessel He thought that maybe the condenser didn t work very efficiently at higher temperatures so maybe that 1s why the cooler was ran at lower temperatures Which brought on more sweating For any future mammalian cell culture runs he suggested using the sparge RING instead of the STONE to inject the air CO2 I don t remember which into the vessel There is a company of the manual used forthe class in another email Now stored in Protocols Directory on Pdrive BobM 12 12 10 STLCC CPLS Morrison 4 11 2014 Page 43 Zj i ul 140 amp CHNINS 8x14 SA L 10x6 EA LL wl a Supply Air In Cubic Feet per Minute Exhaust rate 1595 1455 3050 CFM Cubic feet min 16x16 SA Room 9 000 CF Complete turnover every 3 min 12x16 SA Tri Port Exhause Ve
34. othly swinging the unit In the vertical plane To renew a dried out glass membrane soak it for several hours in the appropriate storage solution It Is recommended to store glass electrodes In dist water combined and refer ence electrodes in the reference solution as under 8 After a long and dry storage period the glass membrana can be reactivated with hydrofluo ric acid 51 340 O73 the elactrade membrane is dipped for 1 min in this solutian followed Ey rinsing with dist water and soak ing for 12 hours in the storage solution Hectrodes with ground sleeve diaphragm slightly lift the ground sleeve so that sore raf electrolyte drops out Check level of electrolyte 5 which should be approx 10 mm below aperture 4 necessary with SM KCGIAQGCI order 51 340 045 for electrodes with Ag AgCl wire 7 aM KCI order 51 340 049 or VISCOLYT order 51 340 235 for high temperature electrodes with AAGENTHAL cartridge Electrodes with gellified electrolyte without refilling port are treated as per 85 1 2 4 5 6 above For storage we recommend a solu tion 3 moll Before callbrating or taking measurements attention should be paid to the instructions of tha relativa pH instrument manufacturer mde mmus cem bho kA Co Bioprocessing pH Probe 1 3L Mettler Unpacking P
35. probe 1359 3107 For 1 3 amp 3 0 Vessels 1 270 Level probe Cable Adapters 1359 3108 For 75 amp 14 0 L Vessels 1 295 11362 25917 1 3 L Vessels 328 Replacement Glass Vessels 1388 8551 3 0 Wessels 328 1273 507 1 3L Heat Blanket Vessel 267 11365 20960 For 7 BL amp 14 0 Vessels 384 1273 5988 3 0 L Heat Blanket Vessel 306 Replacement Foam Level Cablet4dapter 11273 20716 75 L Heat Blanket vessel 645 1 1 381 8014 evel cable fall vessels 302 10 1273 8918 14 0 L Heat Blanket Vessel 929 1273 5042 Fcarn iLevel probe adapter E 1273 98 13 L Water Jacket Vessel 978 compression 11273 20716 3 0 L Water Jacket Vessel 1 040 vu 12 73 3817 75 Water Jacket Wessel 1 315 d 1273 8819 14 0 L Water Jacket Wessel 2 150 STLCC CPLS Morrison 4 11 2014 Page 11 112 7 3 lul 1273 5006 lul 1273 2158 Impe llers 11273 90291 17273 90290 11273 90295 17273 92806 11273 92107 17273 90290 17250 92712 Iul 12 7 3 SC hia 5 0 1273 3201 1273 3200 1273 32 1273 3210 1273 5321 11273 52712 17273 52716 7273 5220 Wes Spare Parts 13 amp 320 vessel Spare Parts Fit 7 5 amp 140 LJ Rushton Impale 1 3 amp 3 0L Rushton Impaler 7 5 L Rushton Impaler 14 0 L Pitched Blade 15 amp 3 0 L Pitched Blade 72 2 14 0 L Upticw Pitched Blade 13 amp 3 0L Down flowy Pitched Blade 275 2 14 0 Down flowy Blade 1
36. red 5 hiereeparger 13 LV Vessel 273 5004 Sintered Porous 3 0L 273 5006 Sintered Porous 7 5 L 273 5006 Sintered Porous hiicrosparger 14 0 L Sampling Asse mb lies 273 9545 Sampling Assembly 1 5 L 273 949 Sampling Assembly 40 L 273 9953 Sampling Assembly 75 L 273 9055 Sampling Assembly 14 0 L Rota r Kits 250 5520 0 205 Kotameter With stand amp tubing 237 3510 amp ice neo us 275 428 Autodave Rack for 75 and 14 01 273 5001 Water 2735 5000 Air IuTT17 2040 2309300 275 9955 273 9659 273 5999 273 5940 POO 4460 26890 2670 Wessel Size 12 3n TE 140 With stand amp tubing Wessels Water Regulator Alter for single system Air Regulator Al ter Fit for single sytem Bearing Housing Gap 10 pack Addition Botte kit 250 m Addition Botte kit 500 ml Addition Botte Holder Fit Silicone Grease for SealaOsings Silicone Tubing clamp Poly sul fore 1 4 0 635 emi Poly sul tome Quick connect ulle 1 4 D ESE emi Ve sse Headplate Parts ulator Kit 4 495 ulator Fit i4 rranifoldzl Price 52 pn Eed has has has 729 753 753 291 291 430 324 _
37. s or cleanroom supplies STLCC CPLS Morrison 4 11 2014 Page 53 BioSafety Gowning Room 120 Layout Ame 18 Wall Hooks for Gowns i heck Y Chairs for Door Putting on footwear Lab 61 STLCC CPLS Morrison 4 11 2014 Page 54 Bioprocessing Facility BRDG R119 Looking North gt 1 1 Ld z m 3 j gt 4 n Sa Lab Gas Tanks and Regulators w Lab Air Valve and Regulator behind controller k B a 7 gt KI OT lt i 4 1 B d uc EP ir i V f Mj FVBP119 01 Primary Controller 8 STLCC CPLS Morrison 4 11 2014 Page 55 Bloprocessing Facility BRDG NW 02 21 2010 STLCC CPLS Morrison 4 11 2014 Page 56 Bioprocessing Facility BRDG STLCC CPLS Morrison 4 11 2014 Looking SE gt Bioprocessing Info CITSen Glucose Sensor Receiver CITSens Bio sensor is a screen printed electrode with an immobilized enzyme and is connected with a cable to the ClTSens Bio beamer The measured data will be transmitted wireless to the 58 receiver ZOMOFI which is connected via RS232 or LAN to a PC ouitable for all st
38. se with acids and dices mes Stertice by 1 Cos ipasety in nor 30 OF papir niccbwe 292 121721 Sad 1 30 miss DW appear dry bor 2 to hes uni char ec x mm He of adi Tygon peristallc 3000 hes Dear for Gow soniai Boa low gas y Smecth bore Good tor vaccas Beide high constant 90 x sec x em Hg Page 40 Bioprocessing Tubing Features Contraints pg 2 2 Designed eer at handi rg toad Therrroptas te In 1 ic bore external bette bom Ten nos sieets marinos wii GINAT Ne 19 makna Te asco Cod At affect taste or and char tor cloth sr carer tha verstoten 56 Fm Ost ff 17176 SPF opere 777 ceat 16 psi far 30 will po veda s gt 17 hi ow and camari dravagt corde tiing general lb arken ty aide crm
39. ta display to settle down can be 10min or more When it does push Set Zero to lock in value Note screen will go back to blank after this is set Rinse probe with DI put in 4 0 solution wait again for Raw Data value to settle then hit Set Span button to lock in value e Return pH probe to storage solution or immediately Autoclave for use Experience has not indicated a recalibration is required after Autoclave STLCC CPLS Morrison 4 11 2014 Page 7 Bioprocessing BioFlo 115 Notes on DO Probe per lan R 8 1 12 n past experience has not calibrated DO probe e Manual says DO probe must be connected to the controller at least 6 hours before use STLCC CPLS Morrison 4 11 2014 Page 8 Bioprocessing BioFlo 115 Notes on Air Feed per lan R 8 11 12 If Lab air 87 psi is available nearby it must be routed through regulator to drop pressure to the 10 12 psi range before connecting to a Bioflo controller and or the vessel peristaltic pump with tubing is generally recommended vs lab air and can be connected directly to the vessel via any available headplate port Always install a filter on the inlet side of the tubing to avoid contamination STLCC CPLS Morrison 4 11 2014 Page 9 Bioprocessing BioFlo 115 Notes on Sampling per lan R 8 16 12 Sanitize hand gloves remove foil on the end of the sampling tube sanitize luerlock port Using sterile syringe extract nominal amount of media v
40. tmess other signs of deterioration B Tubing II there s change in the tubing try in your pump Ch aya emica Chemical compatibility is indicated by five ganara ratings HHH n H D E j RET LEGEND oe zl IM EE Er E mom EF pa ord dater Siena Terr Entec Life Tae Tagore LAL Full amp Lubricant Poissonin PTFE nee gt gt gt B B E L gt gt Z T im C FLEX Fa TM Conese Papier Frar TA 1 Du Port arro k Co Wray Davi Cees Eraser L Rete Weger Pag Si Ooh Pirkarrasce Peniza Dar eum rz 4 i m oe ean CAUTION Varlariang in aressume Dr coneasinatkas mag cause seri oat zr Test dig only ender yaur pa Before chemical mead mata tala Steet MED fatty ari Ferr et _ Ferri salate MH Form chloride WARNING pumps not designa ar intended medical use tha Tron sofa eatment ife nis and ac
41. urn the display to the process fluid temperature fa setpoint change is required use the kew The setpoint range is 5 C to 40 WOT E Ifthe aot ate not used within one minute the controller time out and retum to the process temperature display and any changes not be accepted B Once desired value displayed press the key to conima the change B The display will return to the process fluid terpe rabite indicates the controller display altemating between the two displavs Page 47 Bioprocessing Chiller Reset Filters XXX o 5 FLYS Indicates the total titne the filter has been in use im hours desired press and hold arid then press wi NM to reset DTE value to D di Indicates the total time the di filter carton dee has been in use in hows If desired press and hold and then press V to reset the value to D UNIE Indicates the unt operating time hout This value can not be reset unit c i STLC Figure 6 6 Filter Timer Reset Page 48 Bioprocessing Crossflow System Satorius Stedim Slice 200 17525SYS BT1 Automate optimize and document the crossflow processes The powerful controller of this unit provides your crossflow process with a constant feed rate or constant predefined inlet pressure by adjsuting the speed of the peristaltic pump head Constant Transmembranepressure is also possible Up to five different in
42. y help meet ANSI 249 1 2005 OSHA and NFPA safety requirements and help protect against the most common causes of accidents such as reverse gas flow flashback and hose burn backs Each DGN is 100 tested with actual flashbacks and have built in 100 micron inlet filters reverse flow check valves flame barriers and thermal cut off Inlet G 3 8 Female LH x Outlet G 3 8 Male LH Fuel Gas Service Model DGN STLCC CPLS Morrison 4 11 2014 Page 42 Bioprocessing Notes from Fall2010 Class Instructors Eds These new swabable luer valves were developed as substitutes for needle ports in IV Le I applications They are designed to fill hold and release controlled amounts of fluids on demand Needless access sites are designed for drug delivery l V therapy applications as 245000xxx Series replacements for needle access ports The valve stem and body will mate securely with all SWABABLE VALVES standard luer syringes and luer connectors All materials are Gamma resistant USP Class VI latex free and DEHP free Bodies are available in polycarbonate or in PVC for easy bonding The stems are produced from silicone The valves are compatible with American National Standards for medical material luer taper fitting ISO 594 ANSI HIMA MD 70 1 83 EN20594 and BS 5081 176 The series 245000xxx is a swabable valve utilizing a female luer lock connector and a male luer lock adapter The series 245600xxx is a swabable valve utilizing a female luer l
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