Hardware User`s Manual 5
Contents
1. SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet 5 9 holes box 5 Harvard Apparatus Panlab H 2 Open fuse holder by pulling the flange with a regular screwdriver 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety never open the equipment The power supply WARNING has dangerous voltages 5 9 holes box 6 Panlab H 5 TABLE OF CONTENTS 7 2 7 3 9 9 1 9 2 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION CONTROL UNIT FRONT PANEL CONTROL UNIT REAR PANEL NINE HOLES BOX EQUIPMENT CONNECTIONS WORKING WITH THE EQUIPMENT CONDUNCTING A EXPERIMENT CLEANING THE CAGE 9 2 1 CLEANING THE FLOOR AND TRAY 9 3 9 4 10 11 12 WALLS CLEANING TRNASPARENT DOOR CLEA2. are 2 cages LE507 is for mice and LE509 is for rats 5 9 holes box 8 Panlab H 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL POWER START TELEPHONIC CONNECTORS LED NINE HOLES BOX Pellet Light Feeder Panlab HARVARD APPARATUS Figure 6 Control Unit Front Panel e POWER Red led that indicates that the control unit is ON e START This button can be used to start the experiment if the program requires it The start button can also be used as a marker during the program e TELEPHONIC CONNECTORS There are 3 telephonic connectors o PELLETS Used to connect the pellets dispenser o LIGHT Used to connect the light on the top of the box o FEEDER Used to connect the magnetic detector to detect when the animal introduces its head to eat a pellet e LED With this digital selector that goes from 1 to 9 it is possible to adjust the intensity of the light in the 9 holes 1 is the minimum and 9 is the maximum 5 9 holes box 9 Panlab H 7 2 CONTROL UNIT REAR PANEL POWER REMOTE CAGE MAIN AAA O 000000000000 D00000000000 E e eder e e che Figure 7 Control Unit Rear Panel e POWER Power connector main switch and fuse holder e CAGE DB25 connector used to connect the control unit with the o holes e MAIN DBo female connector used to connect the control unit to the computer serial port if the control unit is the first one Alternatively it is
3. been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void ifimproper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits AN For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed 5 9 holes box 3 Panlab pb e PC Control Some of these instruments are designed to be controlled fro
4. several control units connected in series check the connections MAIN REMOTE see Figure 10 If you work with several control units all of them must be turned on in order no cut MAIN REMOTE communications If you work with multiple control units all ID numbers must be different so that the PCis able to differentiate them If you use a USB to RS 232 check for proper setup on the PC Check that the phone cord that connects the control unit to the pellets dispenser is plugged into the correct port labelled Pellet see Figure 9 Check that the phone cord that connects the control unit with the light is connected in the correct port labelled Light see Figure 9 Check that the phone cord that connects the control unit with the door of the feeder is connected to the correct port labelled Feeder see Figure 9 Check that the DB25 flat cable is connected between the control unit and the experimental cage see Figure 9 Check that the DB25 flat cable is connected between the control unit and the experimental cage see Figure 9 17 Panlab H 11 PREVENTIVE MAINTENANCE 5 9 holes box EXPERIMENT MONTHLY TRANSPARENT DOOR CLEANING WALLSCLEANING CLEANING kes Ea FLOOR AND q PLACING Harvard Apparatus 18 12 SPECIFICATIONS Panlab pb e POWER SUPPLY Input voltage Frequency Fuse Maximum power Conducted noise Universal 100 240V 50 60 Hz 2 fuses 5mm 20m
5. them back into the cage 5 9 holes box 15 Harvard Panlab Apparatus 9 3 WALLS CLEANING To clean the walls you can use a slightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove the foam with a wet cloth and finally dry them with a dry cloth 9 4 TRNASPARENT DOOR CLEANING WARNING In order to clean transparent door never use neither alcohol nor N alcoholic derived products otherwise stripes will appear in the transparent plastic To clean the door you can use a lightly wet cloth and then dry it with a dry cloth If it s too dirty you can wet the cloth with a soapy solution then remove foam with a wet cloth and finally dry it with a dry cloth 5 9 holes box ib 10 TROUBLESHOOTING Panlab H Arie ir This table features instructions to solve the most frequent problems PROBLEM SOLUTION eee e Check the condition of the fuses The equipment is not identified by the program PackWin The pellets dispenser does not work The light of the cage does not work The door of the feeder does not detect when the animal enters the head to eat The equipment does not detect when the animal enters the nose in one of the holes with light The lights do not come on nine holes 5 9 holes box Check that the RS232 cable is connected between the control unit and the serial port of the PC see Figure 9 When working with
6. used to connect the control unit to the REMOTE port of the previous control unit if the unit is not the first one e REMOTE DBg male connector used to connect the control unit to the MAIN port of the next control unit If the control unit is the last one this port is kept free 5 9 holes box 16 Panlab tener 7 3 NINE HOLES BOX PELLET DISPENSER FEEDER LIGHT 9 HOLES PLATFORM TRAY DOOR Figure 8 Nine hole Box e PELLET DISPENSER Supplies pellets to the animal as reinforcement e FEEDER Detects when the animal introduces its head to eat a pellet e LIGHT This light is placed on the top of the box It is used as stimulus e PLATFORM The box is placed on a stainless steel platform e TRAY The tray is easily removable to clean animal excrements e 9HOLES There are o holes Each hole has the following components o LIGHT The intensity of the light can be adjusted in the control unit using the digital selector labelled LED o INFRARED EMITER Emits an infrared beam when the animal introduces its head into the hole the beam is cut and the system detects that the animal has introduced its head into the hole 5 9 holes box Ti Harvard Panlab Apparatus o INFRARED DETECTOR Detects the infrared beam when an animal cuts the beam the system detects that the animal introduces its head into the hole 5 9 holes box 45 Panlab Hever 8 EQUIPMENT CONNECTIONS The LE507 LE509 module is controlled by t
7. E A 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws 5 9 holes box 5 Panlab pb e 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has
8. Hardware User s Manual 5 9 Holes box Operant conditioning References LE507 76 0001 LE509 76 0000 Version V23 10 2014 Panlab s l u International Calls 34 934 750 697 Pa n a b A Harvard C Energ a 112 Domestic Call 934 190 709 Apparatus 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info Qpanlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab pb e 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be A exposed to a hazard Protection terminal ground connection Warning about a hot surface which temperature may exceed 65 C N Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEE
9. NING TROUBLESHOOTING PREVENTIVE MAINTENANCE SPECIFICATIONS 5 9 holes box Harvard Apparatus 10 11 13 15 15 15 15 16 16 17 18 19 Panlab H pb e 6 INTRODUCTION The nine hole box is commonly used to evaluate attention performance in laboratory animals through a visual discrimination task Figure 5 Nine holes box schematic The box is equipped with an arc of 9 contiguous apertures set into the rear wall a house light a food pellet dispenser and a magnetic detector to detect nose pokes into the food holder The holes not used in the experiment can be separately occluded using a metal insert Each hole is equipped with photocell beams and a LED at the rear of the hole providing visual cues specific to each hole Different experimental paradigms can be conducted using the nine hole box For example in the 5 choice serial reaction time task short lasting stimuli are given in pseudo randomized order in one of the holes of the cage commonly holes 1 3 5 7 or 9 If the animal nose pokes into the correct hole it is given a reinforcement pellet If the animal nose pokes into an incorrect hole it is given a time out period no light and the next trial begins The choice accuracy 96 of correct responses gives an idea of the functional integrity of the attention as well as learning processes These parameters are usually altered in animal models of schizophrenia and Alzheimer s disease There
10. ci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canal S re n General Manager pa Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 25 06 2014 5 9 holes box 25 Panlab tener 6 Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment p Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bate
11. den inside the box of the control unit In order to change the ID number in the control unit of the cage you must unscrew the 4 screws on the four legs Before opening control unit you must turn it off and unplug it from the mains 5 9 holes box 14 Panlab pb e 9 WORKING WITH THE EQUIPMENT 9 1 CONDUNCTING A EXPERIMENT 1 Connect the equipment as explained in chapter 8 2 If you will work with several cages at the same time you must set the ID numbers of all control units so that they are different See WARNING at the end of chapter 8 3 Turn all control units on 4 TurnonthePC 5 Once the PC is booted run the program PackWin 6 Open a new protocol or edit an existing one in PackWin Read the user manual of this program for more information 7 Placethe subject in the experimental cage 8 Begin the experiment 9 Once the experiment is finished remove the subject from the experimental cage 10 Turn off all control units and the PC 11 Clean the cage to left it in good conditions for the next experiment 9 2 CLEANING THE CAGE 9 2 1 CLEANING THE FLOOR AND TRAY The floor of the cage is made of metal with holes so that faeces and urine fall into the tray The tray contains the metal floor and collects faeces and urine The set formed by floor and tray can be removed from the cage to be cleaned Once removed from the cage for cleaning you can use soap and water then dry them well before replacing
12. egal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este simbolo no produto o manual de instrug es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuig o para a protecc o do ambiente D 5 9 holes box 25
13. he PackWin computer program through a serial port and the RS 232 protocol Figure 9 Connection between module and computer with 1 cage system The connections necessary for a one cage system connection are listed in the following table FROM CABLE Pellets Dispenser Telephonic cable 1 Telephonic cable 2 Telephonic cable 3 CAGE connector DELTA 25 cable PC serial port MAIN connector RS232 cable 5 9 holes box 13 Panlab computer is able to control up to 10 modules at the same time connecting them a chain using the MAIN and REMOTE connections Figure 10 Example of connection of 3 cages Certain rules must be followed when connecting several modules in a series e All control units must have a different ID number There is a decimal selector inside the control unit that allows selecting values between o and o e The MAIN connector of the first control unit is connected to the computer serial port e The MAIN connector of each control unit is connected to the REMOTE port of the previous control unit e The REMOTE port of each control unit is connected to the MAIN port of the next control unit e The REMOTE port of the last control unit is left free e When several modules are connected in a chain it is not necessary to connect them in the physical order of their identification numbers WARNING Do not confuse intensity light selector labelled LED with the ID selector that is hid
14. ial requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mec nica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2010 EMC EN61326 1 2013 Class B Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE Consequently this product can incorporate the CE marking En cons quence ce produit peut incorporer le marquage CE En representa
15. m 2 250V Fast 30W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing FRONT PANEL PORTS Connector RJ12 Ports LIGHT PELLETS FEEDER BULB Voltage 24V Power o 1W COMUNICATIONS OUTPUT Standard interface RS232C Connector Delta 9 contact connector DIMENSIONS control unit Width x Height x Depth Weight 150 mm 66 mm 250 mm 1 41 kg DIMENSIONS cage LE 507 Width x Height x Depth Weight 442 mm 350 mm 365 mm 6 43kg DIMENSIONS cage LE 509 Width x Height x Depth Weight 442 mm 350 mm 365 mm 7 36kg 5 9 holes box 19 Panlab pb e DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direcci n del fabricante Energ a 112 Manufacturer s address 08940 Cornella de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto NINE HOLE BOX Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 507 LE 509 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essent
16. m a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines 5 9 holes box Panlab pb e 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE
17. r as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El simbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de aparatos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente gt lt y Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique ix Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verb
18. raucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt p Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente PG Nota em Protecc o Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema l
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