Aurora Pre-Run Protocol
Contents
1. the buffer from the sample chamber Select and run the 106 0017 AA D Aurora Pre Run Protocol SP file When the protocol is complete carefully peel off the tape on the extraction well and remove the buffer Rinse the extraction well with 100 ul fresh buffer and discard 4 Refill the extraction well with 60 pl fresh buffer saved in Step 1 and reseal the well with a new piece of tape Remove the buffer from the sample chamber and refill the chamber with the intended sample Continue by selecting an Aurora protocol most appropriate for the sample Troubleshooting Please see the Aurora user manual for more information about troubleshooting machine faults Ordering and support For support for Aurora protocols or cartridges or to order additional cartridges please contact support borealgenomics com This protocol uses cartridge part number 210 0001 CA D SCODA conditions These conditions are pre programmed in the 106 0017 AA D Aurora Pre Run Protocol SP file that accompanies this protocol guide and are intended for reference purposes Note that electric current or power values that slightly exceed these expected values may not indicate a problem Cartridge Sample volume 5 ml Expected sample conductivity NA Pre Run SCODA field strength 70 V cm SCODA cycle period 4s Duration 1 5 hr Expected current 30 45 mA Expected power 9 11 W2. 3 BOREAL GENOMICS Aurora Pre Run Protocol O 2012 Boreal Genomics Inc All rights reserved All trademarks are property of their owners http www borealgenomics com support borealgenomics com 1 604 322 4111 Table of Contents Introdieti0fisieisuda naaca inst reinis id Safety guid lin s ssh a a to ana fera dade a a a a a ataa aaa Running the Aurora protocol ii Troubl shootinE x a lee na Ord ring and support oin tusu dm SEODA CONTAINS A A AAA Introduction This protocol is for removing contaminating DNA in the Aurora disposable cartridges sometimes present in reagents used in their manufacturing The protocol is recommended for any sample that is expected to have low levels of DNA and is run prior to loading your sample and running a subsequent Aurora protocol of your choice Since the pre run concentrates any contaminating DNA molecules within the cartridge it ensures that contaminating DNA is not co purified with the DNA from the sample of interest in the subsequent Aurora protocol Safety guidelines Wear gloves during all stages of the protocol Avoid skin contact with all reagents Appropriate precautions should be taken if hazardous samples are used with the cartridges Running the Aurora protocol 1 Follow the directions in the Aurora Disposable Cartridge Handling Manual 106 0010 to prepare the Aurora Save the buffer removed from each buffer chamber but do not remove
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