ÄKTAdesign Fractionation Kit - GE Healthcare Life Sciences
Contents
1. T Eh A lt i Des Tele El Main i 8 5 0 00 Base CV 0 10 NColumn mb Any The new instruction with the parameter BE 0 00 Block Start_Conditions_GR as variable according to the description below M 0 00 Block Column Equilibration W 0 00 Block Flowthrough Fractionation ai eren 0 00 Base SameAsMain ae 0 00 Fractionation 900 5 itFlowthrough_FracSize ml 0 00 End_block W 0 00 Block Sample_Iniection_ Il 0 00 Block Wash Out Unbound Sample 0 00 Block Stop Flomthrough Fractionation 0 00 OutletValve F1 Stop_Flowthrough_Fractionation 0 00 Base SameAsMain 0 00 Fractionation_Stop_900 0 00 End Bloch 0 00 Block Gradient 0 00 Block Clean After Gradient 0 00 Block Reequilibrate 0 00 End method Eren B i i B E p m sn Outet Valve pos ni x cma tree Click to define a variable name for the instruction parameter 8 Type a variable name e g Outlet Valve pos for the instruction parameter 9 Click OR to insert the parameter in the method 10 Insert an instruction to reset the outlet valve In the figure above the Fractionation_Stop_900 instruction should be replaced with the OutletValve F1 instruction 11 Save the method The outlet valve position can now be selected on the Variables page before starting the method 22 Fractionation Kit User Manual 11 0009 08 Edition AC 4 Method Editor LargeFractions File Edit Block View Help Operation 6 6 1 2 Using the ou2. T Visible in details only Cancel Clear Help Click to define a variable name for the instruction parameter Instructions Peak_Frac_Source Delete Ready i 4 6 Select Frac in the Instructions field 7 Select OutletFractions in the instructions list The instruction parameters allow you to manually select how many fractions to collect the volume of each fraction and which position to start from Fractionation Kit User Manual 11 0009 08 Edition AC 23 6 Operation 8 Click the upper Var button to define a variable name for how many fractions to collect e g No_of_Fracs This variable allows you to control the number of fractions to collect When the number of fractions have been collected the outlet valve is set to start position Note The instruction parameter How_many can also be used to terminate outlet fractionation by setting it to 0 The outlet valve is then reset to start position Variable Name Definition Variable name No_of_fracs T Visible in details only Cancel Clear Help 9 Click OK The defined variable is inserted in the method 10 Click the middle Var button to define a variable name for the size volume of the fractions to collect e g FracSize_Vol This variable allows you to control the volume of the fractions to collect Variable Name Definition Variable name FracSize_Vol O Visible in details only Cancel Clear
3. In KTArp c the fraction collector should be connected to port 1 Note When using Frac 950 in AKTAbasic with strategy version 400 and higher the outlet valve must be manually set to port 2 fraction collector in the method When lower strategy versions are used the outlet valve is automatically set to port 2 Injection valve V1 Pump A Pump B Flow restrictor Outlet valve V4 Fraction collector F3 F4 F5 F6 F7 F8 Tubing no Outlet valve port Connected to 1 2 5 4 2 Fraction collector 1 Waste flask I O center Outlet of the flow restrictor 3 8 Fraction collection flasks In KTArp c the fraction collector should be connected to port 1 16 Fractionation Kit User Manual 11 0009 08 Edition AC 5 Preparation for use Preparation for use 5 5 1 Software configuration 5 1 1 Starting the system 1 Switch on the AKTAdesign chromatography system with the mains switch located at the front left on the system base 2 Check that the computer and printer are switched on 3 Log in see the instrument user manual 5 1 2 Installing the strategy and component in UNICORN 1 Make sure that the correct strategy is installed in your system The strategy version must be 200 or higher To install a new strategy follow the software installation instructions found in the UNICORN User Manuals 2 In UNICORN Main Menu Manager select Administration System Se
4. Help 11 Click OK The defined variable is inserted in the method 12 Click the lower Var button to define a variable name for the outlet valve port to start from e g Start_at_Pos This variable allows you to control the position of the outlet valve from which you start collecting fractions from Variable Name Definition Variable name Start at Pos visible in details only Cancel Clear Help 13 Click OK The defined variable is inserted in the method 14 Highlight the instruction Fractionation_Stop and delete it 15 Save the method The fractionation parameter values can now be changed on the Variables page before starting the method 24 Fractionation Kit User Manual 11 0009 08 Edition AC Operation 6 6 2 UNICORN 4 0 5 01 or higher The Method Wizard will automatically include the functionality of the outlet valve To use the outlet valve in a method select the desired outlet valve options in the Wash Out Unbound Sample and or Elution Fractionation dialogs when creating the method in the Method Wizard Method Wizard for System FPLC EI Wash Out Unbound Sample IV Wash Out Unbound Sample 2 CV Flowthrough Fractionation Outlet Valve F2 gt Set Default Method Wizard for System FPLC r Elution Fractionation Outlet Valve z Number of Fractions 0 OFF 4 v Start at F3 h Fraction Volume 5 100000 ml 10 Set Defau
5. following information carefully as well as all the additional instructions supplied with the components to ensure that the AKTAdesign optional equipment is installed correctly 4 3 Installing the outlet valve 4 3 1 Mounting the valve To mount outlet valve PV 908 see also IV PV 908 Instructions supplied with the valve 1 Set the rotary ID switch at the bottom of the valve to position 4 2 Attach the snap in bracket supplied to the valve using the screws see page 11 in section 4 1 General mounting instructions 3 Attach the valve to the system see page 11 in section 4 1 General mounting instructions Choose a location that minimizes the tubing length to other components connected to the valve 4 Apply an identification number from the labels supplied Place the number so that it is easily readable from the front 4 3 2 Installing the UniNet 2 cables 1 Disconnect the UniNet 2 connector from the component that is closest to the outlet valve 8 Fit the connector in the outlet valve 3 Connect a new UniNet cable supplied between the outlet valve and the nearest component Fractionation Kit User Manual 11 0009 08 Edition AC 15 4 Installation 4 3 3 Connecting the tubing To connect the tubing to the outlet valve 1 Cut the tubing to suitable lengths Use tubing with the correct inner diameter as required by the system Connect the tubing as indicated in the table and figure The figure shows KTAbasic
6. volume must be recalculated and reset when the standard configuration after the UV flow cell is changed WARNING Make sure that the entire system has been flushed thoroughly with distilled water before removing any capillaries or components CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Fractionation Kit User Manual 11 0009 08 Edition AC 11 12 3 Prerequisites Fractionation Kit User Manual 11 0009 08 Edition AC Installation 4 4 Installation 4 1 General mounting instructions The optional outlet valve is mounted in the rails on the side of the modules The valve is attached to the mounting rails with a snap in bracket The bracket is supplied separately with the valve and needs to be fitted as shown below before the valve can be attached Inserting components Removing components 2 Fit the attached mounting bracket with the two screws 1 Loosen the two screws When selecting mounting location for the optional equipment it is important to choose a location which minimizes the length of the capillaries used to connect the optional components to the rest of the system Fractionation Kit User Manual 11 0009 08 Edition AC 13 4 Installation 4 1 1 UniNet connection CAUTION
7. Fractionation Kit User Manual 11 0009 08 Edition AC 27 7 Short instructions 7 2 Reverting to standard configuration Oo CoO N O Flush AKTAdesign with distilled water Disconnect the system in System Control Switch off AKTAdesign Disconnect equipment to be removed from the UniNet chain and reconnect the UniNet chain for the standard configuration Remove capillaries and reconnect the system flow path to the standard configuration Check all connections Start up AKTAdesign Select a method supporting the standard configuration Set up parameters to suit your needs Perform a test run with distilled water to verify the standard configuration in the selected method Fractionation Kit User Manual 11 0009 08 Edition AC Fractionation Rit User Manual 11 0009 08 Edition AC Short instructions 7 29 www gehealthcare com lifescience GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweeden Drop Design FPLC Superloop UNICORN and AKTA are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company Microsoft and Windows are either trademarks or registered trademarks of Microsoft Corporation in the United States and or other countries All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual app
8. GE Healthcare ARTAdesign Fractionation Kit User Manual AKTA Important user information All users must read this entire manual to fully understand the safe use of Fractionation Kit WARNING The WARNING sign highlights instructions that AN must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separatel
9. Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Both the UniNet 1 and the UniNet 2 chain can be interrupted anywhere between the system pump and the termination plug to connect the optional components into the chain UniNet 1 chain UniNet 2 P4 chain to components Fraction collector R 3 Termination plug PC running UNICORN UniNet 1 chain Use the supplied and existing UniNet 2 cables to connect the outlet valve into the UniNet 2 communication link 4 1 2 Valve ID Outlet valve PV 908 ID code switch 14 Fractionation Kit User Manual 11 0009 08 Edition AC Installation 4 The outlet valve PV 908 has a unique ID code which identifies it to the UNICORN control system at system start up This ID code should be checked before installation The ID code for the valve and the corresponding instruction used in UNICORN to control it is as follows Valve function ID code Valve type Instruction in UNICORN Fractionation valve 4 PV 908 OutletValve 4 2 Unpacking Unpack the optional components and check against the supplied packing ist Inspect the items for obvious damage that might have occurred during transportation CAUTION Read the
10. ing a new method or editing an existing method 5 2 Testing the outlet valve 1 Check that the outlet valve added is indicated in the Flow Scheme in UNICORN Verify that the outlet valve is functioning properly by issuing manual commands from UNICORN as follows In System Control select Manual FlowPath Select instruction OutletValve Set the outlet valve to for example position F4 Manually run the system pump with distilled water at approximately 2 ml min Check that water is coming out from port 4 of the outlet valve Fractionation Kit User Manual 11 0009 08 Edition AC 19 20 5 Preparation for use Fractionation Rit User Manual 11 0009 08 Edition AC Operation 6 6 Operation The outlet valve properties are called Outlet Valve in the Flow Scheme view pane and V4_Outlet in the Run Data view pane Two fractionation instructions are available for the outlet valve e OutletValve This instruction parameter turns the outlet valve to one of the eight positions available This makes the instruction suitable for controlling flowthrough fractionation before elution starts The instruction is designated V4_Outlet in the Run Data pane e OutletFractions This instruction has three parameters How Many Volume and Start at This makes the instruction suitable for controlling large volume fractionation min 5 ml during elution Both instructions are independent of using fraction collector Frac 920 or Frac 950 The proced
11. lt Fractionation Kit User Manual 11 0009 08 Edition AC 25 26 6 Operation Fractionation Rit User Manual 11 0009 08 Edition AC Short instructions 7 7 Short instructions The following short instructions are checklists for users who are fully familiar with the safety precautions and operating instructions described in this manual The instructions assume that optional equipment is installed according to the installation instructions 7 1 Converting to optional configurations 1 N DOD OO FEF WN N 10 11 12 13 14 15 Wash the AKTAdesign flow path with distilled water Switch off AKTAdesign Prepare the component Attach the component to AKTAdesign Prepare the necessary capillaries and connectors Mount the capillaries and connectors Connect the component to the UniNet chain and if required to the mains power supply Check all connections Start up AKTAdesign Select the component in System Setup Test the function of the optional equipment manually Create a new method with the Method Wizard and make the appropriate selections to support the optional configuration If the component is not supported by the Method Wizard insert the required instructions in their proper positions in a new or modified method Set up parameters to suit your needs Perform a test run with distilled water to verify the optional configuration in the new or modified method Correct any faults and store the method
12. on collector Frac 920 or Frac 950 the optional outlet valve enables flexible volume fractionation in the same setup The optional outlet valve PV 908 code no 18 1175 86 is available for e AKTAbasic with UNICORN version 3 21 AB or higher e ARTAFPLCTM with UNICORN version 3 20 or higher e AKTApurifier UPC with UNICORN version 3 20 or higher 1 3 Configuration overview The illustration below shows the outlet valve combined with a fraction collector in AKTAbasic Injection valve V1 Flow restrictor Outlet valve V4 Fraction collector F3 F4 F5 FO F7 F8 Fractionation Rit User Manual 11 0009 08 Edition AC Safety 2 2 Safety IMPORTANTI Fractionation is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes e The components are designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water e Operate in accordance with local safety instructions WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING Ensure that the entire chromatographic system has been flushed thoroughly with distilled water before removing any capillaries or components WARNING Always disconnect the power supply before attempting to replace any item on the equipment during maintenance WARNING If there is a risk that large volumes of
13. roval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2005 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB Bjorkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamhire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Huakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 e Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 57606 1619 Fax 01 57606 1627 e Belgium Tel 0800 73 888 Fax 03 272 1637 e Canada Tel 800 463 5800 Fax 800 567 1008 e Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 e Denmark Tel 45 16 2400 Fax 45 16 2424 e Finland amp Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 e France Tel 01 69 35 67 00 Fax 01 69 41 96 77 e Germany Tel 0761 4903 490 Fax 0761 4903 405 e Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Latin America Tel 55 11 3933 7300 Fa
14. spilt liquid may penetrate the casing of the equipment and come into contact with the electrical components immediately switch off the chromatographic system and contact an authorized service technician gt gt PP CAUTION Make sure AKTAdesign is switched off before installing the optional components The mains power to the system must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system gt Fractionation Kit User Manual 11 0009 08 Edition AC 10 2 Safety Fractionation Kit User Manual 11 0009 08 Edition AC Prerequisites 3 3 Prerequisites UNICORN versions must be installed in the computer connected to the chromatography system as listed below e AKTAbasic UNICORN version 3 21 AB or higher e KTAFPLC UNICORN version 3 20 or higher e AKTApurifier UNICORN version 3 20 or higher KTAbasic KTArpLc and AKTApurifier respectively must be installed and fully tested before the optional components are installed See the Installation Guide for your AKTAdesign chromatography system The general procedures for creating and editing methods are described in UNICORN User Manuals Note The system configuration delay
15. tional configurations ee 27 7 2 Reverting to standard configuration 28 Fractionation Kit User Manual 11 0009 08 Edition AC 5 Contents 6 Fractionation Rit User Manual 11 0009 08 Edition AC Introduction 1 1 Introduction 1 1 General AKTAdesign standard system configurations can be changed to optional configurations This flexibility in AKTAdesign system strategies allows the user to enhance already used purification methods and also to develop new more complex methods When optional equipment is connected to a standard chromatographic system new sets of instructions to control the optional equipment become accessible for the user Optional configurations are selected installed and put into operation by the user An optional configuration consists of both hardware components and software instructions Optional configurations are monitored and controlled via methods run by the UNICORN control system in the same way as AKTAdesign standard system configurations 12 Large volume fractionation Outlet valve PV 908 Collection of large fractions can be done automatically by using an additional 8 port outlet valve Fractionation Kit User Manual 11 0009 08 Edition AC 7 1 Introduction This functionality enables the following e Up to 8 vessels of any size can be used to automatically collect fractions over the entire flow rate range of the chromatography system e Combined with a fracti
16. tlet valve for large volume fractionation To prepare a method for large volume fractionation 1 Inthe UNICORN Main Menu select File New Method to create a new method 2 Select System Technique Template and For column Click OK 3 Select View Text instructions to display the text instruction editor 4 Double click on the instruction block where you want to add the outlet valve instruction i e Fractionation 5 Highlight the instruction Fractionation 900 and delete it El BI 0 00 m 0 00 El 0 00 m 0 00 0 00 El El Insert Ay C Pump Peak_FracParametersUV C Flo Peak_FracParametersAD900 watt Fractionation_900 Var ee Change Fractionation Stop 900 AlamstMon JFeedTube 00 e ResetFracNumber 900 e Frac Peak_Fractionation_900 Replace C Watch Peak FracStop 900 C Other Base CV 0 10 Column ml Any Block Start_Conditions Block Column Equilibration The new instruction with the parameters Block Load_Column a ta DG GA DC Unbound Stes as variables according to the description below Block Fractionation oes 0 00 DutletFractions 5 No_of_fracs 10 FracSize_Vol ml F3 iiStart_at_Pos Fractionation 0 00 Base SameAsMain en 0 00 Fractionation 900 5 Eluate_Frac_Size ml 0 00 End_block Block Gradient_Elution Delete this block Block Clean After Gradient ee Block Fractionation_Stop Block Reequilibration End_method Variable Name Definition Variable name No of fracs
17. tup 3 Select System and then Edit Click Component Component x Select components 4 Ll ColumnValve v2 ColumnValve V3 QutletValve V4 BufferYalveA V5 BufferVYalve V6 Valve V7 Valve V8 Valve V9 pH Cell Fraction collector 900 Sample pump P 950 Sample pump P 960 AutoSampler 4 900 AutoS amplerT hermo AutoS ampler 4 905 ADS00 Airsensor P 960 Airsensor 1 Airsensor 2 EE CEE In the Select components list select OutletValve V4 5 Click OK twice and then Close Fractionation Kit User Manual 11 0009 08 Edition AC 17 18 5 Preparation for use The connected valve is automatically recognized by UNICORN The outlet valve properties are designated Outlet Valve in the Flow Scheme view pane and V4_Outlet in the Run Data view pane To view V4_Outlet in the Run Data view pane select the right click command Properties Properties WRun Data Fractionation Rit User Manual 11 0009 08 Edition AC Preparation for use 5 8 Port 8 Port 8 Por YO XX x n Dutikt Buffer i Valve Valve AS 9 Silo W YA IV PV 998 Check that the outlet valve is indicated in the Flow Scheme in UNICORN Change the delay volume in System Settings in the System Control module The instructions associated to the new valve appears when creat
18. ure for using the outlet valve in UNICORN 3 20 3 22 differs from the procedure in UNICORN 4 0 5 01 Therefore the procedures are described in separate sections Note When using Frac 950 in KTAbasic with strategy version 400 and higher the outlet valve must be manually set to port 2 fraction collector in the method When lower strategy versions are used the outlet valve is automatically set to port 2 6 1 UNICORN 3 20 3 22 In UNICORN 3 20 3 22 the Method Wizard does not support the outlet valve The instructions must be manually added to the method 6 1 1 Using the outlet valve for flowthrough fractionation To prepare a method for flowthrough fractionation 1 Inthe UNICORN Main Menu select File New Method to create a new method 2 Select System Technique Template and For column Click OK 3 Select View Text instructions to display the text instruction editor 4 Double click on the instruction block where you want to add the outlet valve instruction i e Flowthrough_Fractionation 5 Highlight the instruction Fractionation_900 and delete it Fractionation Kit User Manual 11 0009 08 Edition AC 21 6 Operation 6 Select OutletValve in the Instructions field The valve instruction parameter allows you to change all eight positions of the outlet valve synchronously 7 Click Var to define a variable The variable allows you to synchronously control the position of the outlet valve to the position you want
19. x 55 11 3933 7304 e Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 e Netherlands Tel 0165 580 410 Fax 0165 580 401 e Norway Tel 815 65 555 Fax 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C L S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 e South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 e Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 e Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 e USA Tel 800 526 3593 Fax 877 295 8102 imagination at work User Manual 11 0009 08 Edition AC 03 2006 li Elanders sterv la 2006
20. y Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to make adequate measures Contents Contents 1 Introduction LL General 7 1 2 _ Large volume fractionation eee 7 1 3 _ Configuration overview eee aaa 8 2 Safety 3 Prerequisites 4 Installation 4 1 General MOUNTING instructions 13 4 1 1 URINCECONDECUO Nit mat meta inter latent te 14 41 2 VAVE IDin aan E AECA OTUCA NES 14 42 Unpacking essnee 15 4 3 Installing the outlet valve nennen 15 4 31 MOUNTING the Valve 15 4 3 2 Installing the UniNet 2 CODES ccccccccssssssssssssssssssssssssssssssssssesseseessssssssssnses 15 4 33 Connecting the tubing nesigura eta iaa 16 5 Preparation for use 51 Software COMM OURO ON caiacs 17 5 11 Starting the SU SECM assets aia onii R 17 5 1 2 Installing the strategy and component in UNICORN 17 5 2 Testing the outlet valve on eccccsscssssssssssscccsssssssssessssccssssssnnssessessee 19 6 Operation 6 1 UNICORN 3 20 3 82 21 6 1 1 Using the outlet valve for flowthrough fractionation 21 6 1 2 Using the outlet valve for large volume fractionation 23 6 2 UNICORN 4 0 5 01 or higher nennen 25 7 Short instructions 7 1 Converting to op
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