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TurboVap 500 User`s Manual

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1. esses 30 Connect the Coolant Tubing Lines 2 dine od Ang ana Eo s tob 32 Fill the Water Bath sise 34 Connect the Power Cord ss 34 Turn ON the Power see 34 Power Up Diagnosis 5 90 000 DAR ERO dent IR nat a Akmang na haute dt Maala 35 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Table of Contents 10 Determine Optimal Concentration Conditions 36 Water Bath Temperature Description sesssssssssem 36 Water Bath Temperature Considerations esesssessseeesse 37 Setting the Water Bath Temperature 37 Condenser Coolant Temperature Considerations esee 37 Fan Speed Considerations ss 38 Selecting the Fan Speed cs it et dedu ute tee AA 38 Selecting the Endpoint and Time Settings 39 When to Use Each Method ssssssssssseememmmmemm 39 Set Time Expired Concentrating for a Set Time aeee eeen 40 Sensor Endpoint Concentrating Until the Sensor Detects the Endpoint 40 Set Time After Sensor Concentrating for a Set Time Past Endpoint Detection 41 Stop Pressed Concentrating Until the Start Stop Button is Pressed 41 General Guidelines For Achieving the Best Recoveries eeeeeeeeeeeeeeeeee tees 42 Achieving Excellent Recoveries with 1 0mL Sample Stem Tubes 42 Final Concentration Co
2. Check for the conditions described After these conditions are ruled out replace the motor P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Specifications 68 Specifications Capacity 2 samples up to 500mL each Water Bath ambient to 95 C Temperature Can cool below ambient by using the Range evaporation as a cooling source Sensors must be removed to use above 60 C Condenser 4 C 20 C Coolant Using low temperatures without freezing will Temperature increase the concentration rate and improve Range solvent reclamation and sample recovery Dimensions Height 24 0 60 9 cm Width 21 2 53 8 cm Depth 11 9 30 2 cm Weight 40 5 Ibs 18 47 Kg empty water bath Operating 59 to 95 F 15 to 35 C Temperature Operating 0 to 85 relative humidity non condensing Humidity Storage 50 to 122 F 10 to 50 C Temperature Storage 0 to 85 relative humidity non condensing Humidity Altitude Up to 2000M Indoor Use Only Input Voltage 100 120VAC 10 220 240 VAC 10 Line 50 60 Hz Frequency Fuses 100 120VAC 1 10A T250V P N 39164 220 240 VAC 2 5A T250V P N 44412 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Specifications 69 Containers Biotage glass concentration tubes with 0 5mL or 1 0 mL stem Pyrex can be baked at up to 450 C When using sensor endpoint d
3. When the Endpoint is Reached When a cell reaches the selected endpoint the green DONE light blinks and the alarm sounds briefly every thirty seconds To Silence the Alarm Press the Start Stop button twice If running manually the amber Stopped light comes on after you press the Start Stop button Disassembling the Closed Cell 1 Grasp the motor assembly and condenser as a unit and separate the condenser from the sample tube 2 Separate the motor assembly cell cap from the condenser and place the motor assembly back on the bracket Place the condenser on the holder 3 Remove the sample tube from the water bath and place it into the holding rack NOTE 1 Prompt removal of the completed sample tube is important Highly volatile samples can be lost if allowed to sit for an extended period of time 4 Replace the plastic water bath covers P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Operation 47 Removing the Sample from the Tube The gas vortex shearing action automatically rinses the vertical side walls as evaporation occurs but for optimum recoveries you should rinse the lower angled portion of the sample tube to recover any sample left behind See Achieving Excellent Recoveries with 1 0mL Sample Stem Tubes on page 42 Turning the Power Off When the unit is no longer in use 1 Turn the power off 2 Turn off the chiller recirculation unit 3 Place the plastic covers on each c
4. P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 40 Set Time Expired Concentrating for a Set Time To concentrate for an exact length of time use the Set Time Expired option 1 Press the Concentrate Until button until Set Time Expired is selected 2 Use the Set Time buttons to select the desired time in minutes for concentration 3 Press the Start Stop button for the cell position s Reading the Time Display during a Timed Concentration The Time display shows the actual elapsed time alternately for each cell L precedes the left cell value and r precedes the right cell value Sensor Endpoint Concentrating Until the Sensor Detects the Endpoint To use the optical sensors to concentrate to a volume of either 1 0mL or 0 5mL select the Sensor Endpoint option The volume at which concentration stops depends on the stem length of the concentrator tube To set up a Sensor Endpoint concentration 1 Press the Concentrate Until button until Sensor Endpoint is selected 2 Press the Start Stop button for the cell position s Reading the Time Display during a Sensor Endpoint Concentration The Time display shows SE Sensor Endpoint Considerations Two conditions affect optical sensing 1 Water Bath Temperature Remove the sensors if the water bath temperature is set above 60 C See page 54 for detailed instructions 2 Sample Clarity Clear
5. Tools and parts required e flatblade screwdriver four screws motor mounting bracket e two post covers Figure 7 Motor Bracket Parts To install the motor mounting bracket 1 Position the mounting bracket against the back of the concentrator with the two cutouts in the top of the bracket toward the front of the concentrator Line up the two holes on each bracket post with the two holes on each side of the rear service panel as shown in Figure 8 on page 26 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 26 Install the Motor Mounting Bracket Continued Mounting Bracket Figure 8 Mounting Bracket Position Back of TurboVap 2 Using a flatblade screwdriver secure the mounting bracket with the screws provided 3 Place the two motor assemblies onto the top of the mounting bracket 4 Thread the motor wires into the channels on the mounting bracket posts 5 Snap the post covers in place over each channel lining up the dimples with the holes in the channel Assemble the Motor Cell Cap and Fan Blade Tools and parts required 0 05 Allen wrench supplied small and large PTFE seals fan blades two glass cell caps two motors P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 27 Assemble the Motor Cell Cap and Fan Blade Continued 1 Insert the small PTFE seals into the cavities of the larger white PTFE sea
6. Unit inoperative Power switch off Check that power switch is turned on Loose power cord Check that power cord is fully seated Faulty fuse Replace fuse Faulty control board Replace control board or contact Biotage 1 Point Support see page 4 Turbid bath water Bacterial growth Clean and use Clear Bath additive Leaking vapors ground glass condenser joints require PTFE sleeves See Add PTFE Sleeves to the Ground Glass Fittings on page 28 Difficulty separating glassware PTFE sleeves on ground glass joints not installed See Add PTFE Sleeves to the Ground Glass Fittings on page 28 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 63 Procedure for Dislodging Bubbles As the water bath begins to warm up air comes out of solution and bubbles may form around the sensor s optics Also whenever you have removed the water bath rack for servicing replacing the rack may trap air bubbles In most cases sample tube insertion frees any bubbles trapped around the sensor s optics In case bubbles trapped in the sensor s optics affect the concentration process described in the Diagnostic chart perform the following steps to dislodge the bubbles 1 Grasp the sample tube and using a pumping motion raise and lower the tube about one inch Pump the tube up and down several times 2 Perform step 1 on the other cell positio
7. Installation Tool WU 3 PTFE Sleeve Figure 14 Installing the PTFE Sleeves 3 Pull the sleeve along the tool until it fits onto the cell cap Remove the tool 4 Repeatthis procedure to add a sleeve onto the other cell cap and onto both condensers as shown in Figure 15 on page 29 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 29 Add PTFE Sleeves to the Ground Glass Fittings Continued Condenser Ground Glass Joint PTFE Sleeve Figure 15 PTFE Sleeve on Condenser Assemble the Closed Cell Glassware Assemble the glassware as shown in Figure 16 and place the assemblies into the water bath racks Motor Assembly Cell Cap Fan Blade N Coolant Outlet Condenser N Coolant Inlet Reclaimed Solvent Drain eS Concentrator Tube Figure 16 Assembled Closed Cell Glassware P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 30 Select an Appropriate Waste Container Consider the following recommendations when selecting a waste container for each condenser AAL solvent bottle placed on the floor is an ideal waste container NOTE Follow an appropriate chemical hygiene plan for your laboratory a second overflow waste container may be required e Use a waste container with a standard U S 38 430 thread pattern A cap insert modifier is included for use with European 38 430 bottles e Select a container that will allow
8. recovery temperature 36 removing from tube 47 Sample tube cleaning nasasapawan ia RR 50 consumables 49 maintenance 50 Order 44 0400 54 oe do eg Ut 70 Darts aic p RE EGORGSGEGGR UO EROR 21 SMOVING 28 88 sos das tee de 46 removing sample 47 SE posse d MR ec 18 40 41 45 61 Seals installing uad ac rar ic ie ede 27 OFdeTITguu se Les eo cent de de 72 r eplagirig waa o ac na FCR RC 49 Selecting concentration conditions 36 location ss dod edo C doe ROI n 25 Sensor Endpoint considerations 40 endpoint selection 18 MALA AA 40 Sensors actuate prematurely 40 consumables 49 disconnecting 56 expected values 66 features 15 NANO a c wa a ton eo or operating without 57 HBD IGCITIO 0 4240 ERRORS ERROR 54 iBSUDQs uas doc ATAT 65 unplugging 55 wear life 49 Service panel 16 reMOV Ng Es oases e ER S eve 55 FEDIACING is suse e xc Eod 57 P N C128100 Rev 02 TurboVap 500 User s Manual Set Time After Sensor considerations 41 endpoint selection 18 USING np 41 Set Time Expired endpoint selection 18 USING cnt AWA 40 Shearing action 12 Siphon changing the water 50 cleaning the bath 53 Ordering a e
9. 0mL or 0 5mL are available P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 22 Installing the TurboVap 500 Unpacking the TurboVap 500 Unpack the shipping container and verify that all items listed below are present If anything is damaged or missing contact the Biotage 1 Point Support Center see page 4 Parts Supplied The Biotage TurboVap 500 Closed Cell Concentrator comes with the following parts e Concentrator Unit two Motor Assemblies e Motor Mounting Bracket Kit Bracket two Post Covers four screws Glassware in boxes two Condensers two Cell Caps two Sample Tubes with 1 0mL stems PTFE Sleeve Kit Sleeve Installation Tool four PTFE Sleeves Fan Blade Kit two Fan Blade Assemblies two sets of PTFE Seals 2 large and 2 small seals Allen Wrench 0 050 two Drain Tubing Kits each containing Drain Tubing assembly with two different tubings 1 8 I D Bevaline V tubings 5 feet for vent line two Tubing Clips Waste Cap with U S 38 430 threads Cap Insert Insert Modifier optional use Vent Line Restrictors Instructions P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 23 Parts Supplied Continued Condenser Cooling Tubing Kit four Condenser coolant line tubing lengths with screw on cap and Quick disconnect fitting two Quick disconnect fittings 1 4 bar
10. 500 without water in the water bath P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 16 TurboVap 500 Rear Alarm Volume Control Service Panel Power Entry Port Figure 3 TurboVap 500 Hardware Rear View Fan Cools the internal components Do not block the fan opening Alarm Volume Control Adjusts the loudness of the buzzer that signals when the endpoint is reached or a button is pressed Power Entry Port Supplies power to the unit and houses the fuses WARNING AAA To avoid the risk of fire or electrical shock plug the power cord securely into a properly grounded outlet Replace fuses ONLY with the same type and rating Service Panel Provides access to the motor and sensor connectors WARNING AA To avoid electrical shock and other injuries turn OFF the power and unplug the power cord before removing the service panel P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 17 TurboVap 500 Side Quick disconnect Inlet and Outlet Figure 4 TurboVap 500 Side View Quick disconnect Inlet and Outlet Supplies coolant tap water or chilled water to the condensers Front Panel The control panel allows you to control concentration in the two tube positions Samples are not required in each position The microprocessor constantly monitors the control panel settings enabling you to change the settings at any time during ev
11. 55 to access the sensor mounts on the bottom of the rack Observe the lengths of the sensor wires and route both sensors to their mounts making sure that the holes in the sensor align with the holes in the mount Use the screws previously removed to fasten the sensor to the mount Make sure that the sensor s wires run in the same direction as the existing sensors Fit the sensor wires into the rack s retaining clips If you are installing new sensors feed the connector through the back corner of the unit and plug the connector into the properly numbered receptacle on the interconnect PC board Replace the rack in the bath making sure the holes on the top of the rack align with the holes on the upper lip of the bath Position the black top plate over the rack making sure the cutout on the underside of the plate faces the back of the unit Secure the top plate with four Phillips screws Replace the rear service panel and secure with the flathead screws if the panel was removed Fill the water bath plug in the power cord turn the unit on and dislodge any bubbles that may have formed around the sensor optics Confirm correct sensor placement and connection by completing the Cell Diagnostics on page 65 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 58 Long Term Storage If the TurboVap will be stored for an extended period of time 1 Turn the unit off and unplug the power cord Remove all gla
12. Po Entree Output Ie Sortie Equipment Yellow Caution risk of danger labels are color Red Stop coded Blue Mandatory action Les tiquettes Green Safe condition or information de l appareil Jaune Attention danger potentiel sont cod es Rouge Arr ter couleur Bleu Intervention obligatoire Vert Condition s re ou informations de s curit e Helpful hints additional information Conseils utiles informations suppl mentaires P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Table of Contents 9 Table of Contents Preface eerte eate NAA RUEYRRCBNE REP REL SERRE REX ARER ER EERYRRRAUERIE SEE NER eee ye Peek desc ses ses 3 Miro duct NANANA NAA AABANG 12 Gas Vortex Shearing AGUO v tea eode Nana pa ALA 12 Automated Concentration ss 13 AK 13 s li m t 13 CONVENIENCE eee RH IE 14 Mild Conditions ne teer e xe Ene Re RR RR RH EX EORR XR RE Ra Ra tede ER 14 Multiple Sample Processing sesssssessssssesseeeemmem eene 14 Independent Operation 14 Requires No Hood iiit i Pte de AN rat at pa Ped x pq viv Eas 14 Vapor Removal og ERE ma LE RR EX EORR PEET NAG 14 TurboVap 500 Hardware le csvetesctesessescecescetsstvssrtcscheevercssccecsstecdeoscnecesiecssd 15 T rboVap 500 Fronda Acc bake a res Sands diane Weed sure TANG ANAN 15 T rboVap 500 Rear tins re otn ee EF Lea enit e NAA 16 TurboVap 500 Side ss 17 Front Panel e e RET RARE eA
13. about 1 Liter of distilled water into the bath Add 15 drops of Clear Bath Add more distilled water until the water level is as high as the initial solvent level in the sample tube without causing an overflow when both tubes are in position Plug in the power cord and turn the power on Allow 20 30 minutes for the bath to reach temperature for the air air to come out of solution and for most of the bubbles to dissipate Dislodge any remaining bubbles see Procedure for Dislodging Bubbles on page 63 Removing and Replacing the Sensors This section describes how to remove and replace the optical sensors You must remove all of the sensors from the bath whenever the water bath temperature is set at or above 60 C Normal maintenance procedures require replacing each worn sensor as required You should complete the Sensor Test in Cell Diagnostics on page 65 whenever you replace a sensor Tools Required Tools used in this procedure are P N C128100 Rev 02 Flatblade screwdriver Phillips head screwdriver Wire cutters TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 55 Remove the Covers and Unplug the Connectors Follow these disassembly steps before removing the optical sensors 1 Remove all glassware from the unit 2 Turn the concentrator s power off and unplug the power cord 3 Using the Phillips screwdriver remove the two condenser holding cups and the four screws that secure the bath cov
14. approuv par l entit responsable de la conformit peut annuler l autorisation d op rer l appareil accord e l utilisateur Table of Symbols Table 1 contains symbols that identify particularly important information and alert you to the presence of hazards These symbols may appear in this manual and or on the product it describes Table 1 Important Symbols Symbol Description Symbole Description DANGER An imminently hazardous situation which if not D avoided will result in death or serious injury DANGER Situation pr sentant un danger imminent qui s il n est pas limin peut entrainer des blessures graves voire la mort WARNING Caution risk of danger Refer to the User s documentation AVERTISSEMENT Attention danger potentiel Se reporter la documentation de l utilisateur NOTE A cautionary statement an operating tip or maintenance suggestion may result in instrument damage if not followed REMARQUE nonc indiquant une pr caution prendre un conseil de fonctionnement ou une suggestion d entretien son non respect peut provoquer des dommages l instrument Hazardous voltage risk of shock injury Tension dangereuse risque de blessure par lectrocution Crush hazard Risk of body parts hair jewelry or clothing getting caught in a moving part Danger d crasement Faire attention que les parties corporelles les cheveux les bijoux ou les v tements ne soient pas pri
15. power entry port 6 Plug in the power cord and turn on the AC power P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 53 Cleaning the Water Bath Turbid water may affect sensor operation The addition of Clear Bath a biological growth retardant helps eliminate the need for frequent cleaning Keep the water in the bath clean to ensure excellent recoveries Tools required Phillips head screwdriver Water bath siphon supplied with unit Cleaning supplies Clear Bath To clean the water bath Turn the unit OFF and unplug the power cord 2 Remove all glassware 3 Remove the condenser holding cups and the top plate 4 Carefully lift the rack out of the bath 5 Siphon the water out of the bath by following these steps a Close siphon bulb vent b Place siphon s suction tube in water bath c Place drain tubing in sink d Squeeze siphon bulb to start 6 Wipe and rinse the bath walls Siphon the rinse water out of the bath 7 Clean the rack by rinsing with water 8 Replace the rack in the water bath 9 Replace the top plate and holding cups WARNING AA Fire and burn hazard Do NOT operate the TurboVap 500 without water in the water bath P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 54 Refilling the Water Bath To refill the water bath 1 2 3 4 Place one concentrator tube in position in the bath Pour
16. slowly darkening or opaque at 940 nm samples reliably operate when using the endpoint detector Samples which darken too rapidly as concentration proceeds may activate the sensor prematurely If this happens press the Start Stop button for the sensor to start with a new baseline reading and continue concentration P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 41 If starting a new baseline is unacceptable determine concentration time and use the Set Time Expired option or operate manually by using the Stop Pressed selection Set Time After Sensor Concentrating for a Set Time Past Endpoint Detection To continue concentration after the sensor detects a 0 5mL or 1 0mL endpoint depending on glassware select the Set Time After Sensor option To set up a Set Time After Sensor concentration 1 Press the Concentrate Until button until Set Time After Sensor is selected 2 Use the Set Time buttons to set the desired time in minutes for concentration to continue after endpoint detection 3 Press the Start Stop button for the cell position s Reading the Time Display during a Set Time After Sensor Concentration The Time display shows SE until the endpoint is reached Once the endpoint is reached the actual elapsed time after the endpoint is displayed for each cell Set Time After Sensor Endpoint Considerations The same considerations exist for this selection as for t
17. the optical density of the sample to assure that darkening or brightening samples do not stop the concentration prematurely Once the sample passes below the optical sensor and remains below the sensor for a few seconds the TurboVap 500 stops the concentration or starts the timer to concentrate for additional time The stem size of the concentrator tubes determines the final volume when using the sensor endpoint Tubes with a 0 5 mL stem concentrate to 0 5 mL tubes with a 1 0 mL stem concentrate to 0 75 mL and have a calibrated mark on the tube for you to reconstitute to 1 0 mL Adjustable The TurboVap 500 enables you to concentrate for a set time use the sensor endpoint to detect a level of 0 5 mL or 0 75 mL continue concentrating for a set time after reaching the sensor endpoint concentrate until stopped manually optimize the concentration rate by adjusting the water bath temperature and the fan speed NOTE 1 Time temperature and fan speed may be changed at any time P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Introduction 14 Convenience The TurboVap 500 enables you to start a run of samples and leave the instrument unattended When the concentration has finished the fans turn off automatically and the concentrator sounds an alarm every 30 seconds until the samples are removed or Start Stop is pressed Mild Conditions The water bath provides sufficient warming without harming
18. to the external coolant supply OUT to Coolant Supply OUT Figure 23 Coolant Supply Out Connection P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 33 Connect the Coolant Tubing Lines Continued 3 Insert the barbed fitting on the other length of tubing into the TurboVap 500 coolant supply OUT port on the side of the TurboVap 500 and connect the other end to the external coolant supply RETURN Cu OUT IN to Coolant Supply RETURN Figure 24 Coolant Supply Return Connection 4 Connect the condensers to the unit s coolant IN and OUT ports on the top of the TurboVap 500 Figure 25 Condenser Tubing a Connect the screw on caps on the tubing to the condenser IN and OUT fittings b Attach the top condenser fitting coolant OUT to the TurboVap 500 OUT and the lower condenser fitting coolant IN to the TurboVap 500 IN as shown in Figure 26 c Place the condensers on the resting blocks Place the concentrator tubes into the Auxiliary Rack Figure 26 Condenser Connections viewed from the back P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 34 Fill the Water Bath When filling the water bath make sure that the level of water in the bath is as high as the initial solvent level in the sample tube placing the sample tubes in the water bath does not cause an overflow To fill the water bath 1 Pour about 1 Liter of di
19. usi dox ene ee wR CR 17 supplied 22 Power required 4 x 8534288 at os 24 specifications 68 switch 15 troubleshooting 62 turning OFF ca ecd treu PERS 47 arro ON ss vs e RO Rp deed 34 Power entry port aaa 16 changing fuses 52 Power Up diagnostics 64 ON Startup aa 23 d dpa Kaon od 35 Preventative maintenance 49 PTFE seals installing 43i oir det ded ocio 27 replacement 72 PTFE sleeve installation tool 72 nalng RED 28 ordering kila xa du hc Rhe 72 PTFE washer ordering 72 Pushwheel 18 Ie SUDO 4 614 600 pag KA ode poto o ed 64 Pyrex container specifications 69 R So gd de obra a aeo 18 40 41 45 Racks additional 49 replacing xs cueste ped 56 Recovery achieving best 42 using 1 0 mL sample tubes 42 Removing bath cover 55 sample from tube 47 sample tube 46 SensO S 4d acd EE X Oc 08 Ka 54 Replacement kits 71 Replacement parts 70 Replacing USOS EL ete ses ici 52 PACK ss ess ssiausesenartas 56 SENSO Snie gais Sos KE amies 54 Biotage AB Routine maintenance 49 rpm considerations 38 fan speed selections 19 Se lali iue ique d d etur ds 38 S Sample Addi a SL sn sr se 45 dcn mers 40
20. 16 side view 17 Helical flow 12 IRON toi do PEE aed eg dG 61 Holding GUPS iu auacd BAKA eee GA 15 H rnidlly s kA BAKA ecd PAA HAKA 68 Biotage AB l In Process lights sse eR 18 Inlet connector 17 connecting esses RR RR me 32 Installation 22 Installing drain tubing is aco cec eta 30 mounting bracket 25 SENSONS 4s ap MORE A ROGERUS 57 TurboVap 500 25 Introduction 12 K Kits ordering replacement kits 71 L ee ed enin 18 40 41 45 Lights testing Ka aa dou cs p beg 64 Worm 61 M Maintenance 48 daily ctct eo ee eer ned a yah a 48 glassware 50 FOULITIG i i dee o pe ORC UR RR RC 49 water bath 50 Motor assembling ss e dc eH 26 45 expected current values 67 installing mounting bracket 25 mounting bracket 15 Darts same hee see thee ane 20 KH 4 UTE 67 O Operating without sensors 57 Operation 44 preparing 44 Outlet connector 17 Connecting sd aoc eds 32 P Panel cleaning 48 Part numbers 70 Parts sensuel eed exe 15 closed cell 20 front panel 17 P N C128100 Rev 02 TurboVap 500 User s Manual front view 15 rear VIEW ag aod E Poe loe 16 SIde VIEW s
21. 28100 Rev 02 TurboVap 500 User s Manual Biotage AB Introduction 12 Introduction The Biotage TurboVap 500 Closed Cell Concentrator is a microprocessor controlled concentrator that provides automated sample concentration using a helical gas flow and sensor endpoint detection technology Figure 1 The TurboVap 500 Gas Vortex Shearing Action The concentrator uses a patented gas vortex shearing action that maintains high concentration rates regardless of sample height in the tubes A helical flow of air see Figure 1 is created by a motor driven fan The helical flow sets up a vortexing action that promotes sample homogeneity and continuous rinsing of the tube wall The solvent vapors rise enter the condenser condense on the side walls and then run out the drain into a suitable waste or reclamation container P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Introduction 13 Automated Concentration The TurboVap 500 uses an integrated microprocessor to automatically time the concentration control the water bath temperature turn off the fans and perform operational diagnostics Sensors Optical sensors in the water bath enable the TurboVap 500 to automatically stop the concentration when the sample level in the tube passes below the optical sensor The final volume of the concentration either 0 5 mL or 0 75 mL depends on the size of the sample tube being used The optical sensor tracks any slow changes in
22. T TU ER ER ER XX PRAE etes 17 Concentrate Until Options 18 Cell Selections and Functions renn 19 Fan Speed Selections and Functions 19 Closed Cell Parts and Functions kkaaa kanan 20 Installing the TurboVap 500 asemana nn waaa kNKKA NN NA KANA ANAKAN KK SANA NN NANANA KAKANAN KKNK DAAN 22 Unpacking the TurboVap 500 22 Parts SUppliGd i tener nA vid iv oad der e ra epe er Fere NAAN 22 Other Required Items sse ene eene nene ene nnn rrr rrr 23 Tools Needed e oe ee re RE RE AE Ea ROERR indemne ERR nes 23 Site Preparation Requirements 23 Installing the TurboVap 500015 er tti NAN kaa ha 25 Select the Location for the TurboVap 500 25 Clean the Glassware 25 Remove the Sensors for Operation Above 60 C Xa 25 Install the Motor Mounting Bracket 25 Assemble the Motor Cell Cap and Fan Blade 26 Add PTFE Sleeves to the Ground Glass Fittings 220 0002mssssaaa swan 28 Assemble the Closed Cell Glassware 7000000000000 asanasaanaa sanan 29 Select an Appropriate Waste Container 30 Connect the Drain Solvent Reclamation Tubing
23. _ Biotage TurboVap 500 Closed Cell Concentrator User s Manual CE Copyright 2010 Biotage AB All rights reserved P N C128100 Rev 02 Copyright Trademarks Content Preface 3 Preface This manual is published by Biotage AB 10430 Harris Oaks Blvd Suite C Charlotte NC 28269 U S A Copyright 2010 Biotage AB All rights reserved Reproduction by any means or in any form of this manual or the products it describes is prohibited Biotage and TurboVap are trademarks of Biotage Microsoft and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries All other trademarks and registered trademarks are the property of their respective holders The information in this manual may contain typographical errors or technical inaccuracies and is subject to change without notice Modifications may also be made to the product described in this manual at any time Proper Equipment Operation The TurboVap 500 Closed Cell Concentrator uses a patented gas vortex shearing technique to concentrate samples quickly It is a microprocessor controlled instrument for simultaneous automated concentration of multiple samples with unattended operation convenience and speed It is the customer s responsibility to design suitable methods for evaporation of their own samples WARNINGS AA The TurboVap 500 is NOT designed for in vitro testing or for use with highly corrosi
24. a gravity feed fluid flow down the tube Select at least a 500mL container The waste container must hold at least the total amount of evaporating solvent plus an additional 100 mL Connect the Drain Solvent Reclamation Tubing The contents of the Drain Tubing Kit are shown in Figure 17 DUA NANG NT INSTRUCTIONS Wr De Tang Yen UV wed Coll Cteconiralor Figure 17 Drain Tubing Kit To connect the drain tubing 1 Select a Solvent Reclamation Waste Container for each condenser Place the containers below the condensers 2 With the condensers and concentrator tubes assembled and in place in the water bath racks attach the screw on fitting end of the drain tubing line assembly to the reclaimed solvent drain fitting the lowest fitting on the condenser 3 Route the drain lines to the waste containers and secure the tubing to the rear panel of the unit a Slide the tubing clips onto the drain tubing P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 31 Connect the Drain Solvent Reclamation Tubing Continued b Fasten the clips to the service panel using the lower left and lower center service panel screws as shown in Figure 18 Figure 18 Tubing Clips 4 Place the cap insert into the supplied plastic cap see Figure 19 screw the cap onto the waste container and attach the drain tubing to the large barb fitting Cap Cap Insert Figure 19 Cap Insert 5 Connect the sm
25. aae sn a RR deen 72 Site preparation 23 Software diagnostics 63 Software version viewing 64 Solvent evaporation 51 recovering st pe haki Asa Piu 42 NNSING aeu 4e45 5442208 d20ay 51 Solvent reclamation 30 specifications 69 Solvent vapors 12 14 Spare parts 70 71 Specifications 68 Start Stop button 19 Starting concentration 45 new baseline reading 40 TurboVap 44 Stop Pressed endpoint selection 18 USING saraap BR r Ero Ee 41 Stopped light 19 46 Stopping concentration 46 troubleshooting 60 T lap waters accsaa cur COR d s 17 required es acp arp d ve de tits 23 USING es rte ste e C rt 37 Temperature condenser coolant 37 condenser coolant specifications 68 coolant considerations 37 ui ac beet ee Rate oce eoe 18 high temperatures 54 Biotage AB Operallngss savais dors 68 pushwheel 18 setting water bath 37 specifications 68 SO ae M cies re eRe we 68 testing pushwheel 64 troubleshooting 60 troubleshooting display 61 Tests cell diagnostics 65 display test 64 MOLON sirara rienak Bora on 67 pushwheel connectio
26. able to absorb approximately 60 watts sample Bath Capacity 6 4L maximum distilled water DO NOT overfill WARNING AA Burn hazard DO NOT move the unit when the bath is full Clear Bath Additive 2 oz kit supplied with unit WARNING AAAA Exposure to liquids may cause bacterial or viral hazards Liquids can also be corrosive flammable and or toxic Use good laboratory operating procedures when dealing with liquids Refer to the MSDS Material Safety Data Sheet for detailed information Avoid direct contact with liquids Wear protective gloves and safety glasses Dispose of liquids and containers properly Do not overfill containers P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 25 Installing the TurboVap 500 Select the Location for the TurboVap 500 Select a flat stable dry bench location within reach of the required power and water sources Clean the Glassware Ensure that the TurboVap 500 closed cell concentrator glassware is clean according to your standard laboratory practices Methods of Cleaning the Glassware on page 50 describes some common cleaning methods Remove the Sensors for Operation Above 60 C If you will be operating the TurboVap 500 at water bath temperatures above 60 C you must remove the sensors See Removing and Replacing the Sensors on page 54 for detailed instructions on removing the sensors Install the Motor Mounting Bracket
27. all ID vent tubing to the small barb on the cap insert 6 Insert the vent line restrictor into the open end of the vent tubing and route the vent tubing line to a hood or outside ventilation source T Vent Line Restrictor Drain Line from Condenser EE Vent Line with Restrictor Figure 20 Vent Line Restrictor 7 Ifthe cap and insert don t fit tightly onto the waste container add the insert modifier to the cap insert P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 32 Connect the Coolant Tubing Lines The coolant system assembly requires twelve tubing line connections Note Consult the instructions for your chiller as required Use the four tubing assemblies to make the connections between the TurboVap 500 condenser s coolant inlet and outlets and the unit s coolant IN and OUT connections Use the 15 length of supplied 14 I D tubing and two 74 barb quick disconnect fittings to connect the TurboVap 500 to the external coolant supply not supplied 1 Connect the TurboVap 500 to the external coolant supply Figure 21 Coolant Tubing a Cutthe 15 4 I D tubing in half b Attach one 74 barb quick disconnect fitting to one end of each tubing length um X Figure 22 Coolant Line Fitting 2 Insert the barbed fitting on one of the lengths of tubing into the TurboVap 500 coolant supply INLET port on the side of the TurboVap 500 and connect the other end
28. an and free of solvent Solvent in the water bath can quickly damage the sensors To check sensor operation follow the Cell Diagnostics on page 65 As routine maintenance run the sensor diagnostics periodically to confirm proper operation P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 50 Water Bath Maintenance Always use distilled or de ionized water Add Clear Bath additive whenever the water is changed WARNING AAAA Exposure to liquids may cause bacterial or viral hazards Liquids can also be corrosive flammable and or toxic Use good laboratory operating procedures Refer to the MSDS Material Safety Data Sheet for detailed information Avoid direct contact with liquids Wear protective gloves and safety glasses Dispose of liquid and container properly Do not overfill containers Periodically change the water in the bath using the siphon provided With average use change the water at least every 2 months or sooner if it becomes cloudy The cleaner the water is the better the sensors operate If residue begins to build up in the bath the sensors may give false readings Glassware Maintenance Clean and rinse the TurboVap sample tubes following your laboratory protocol Residue remaining on either the inside or outside of the sample tube stem can impact sensor operation The condenser glassware does not usually require cleaning However high sample levels may r
29. and clothing away from the fans Note The fan speed button is also used to run diagnostics see Software Diagnostics on page 63 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 20 Closed Cell Parts and Functions Motor Assembly Cell Cap Fan Blade RE N Coolant Outlet Condenser Reclaimed Solvent Drain Concentrator Tube Figure 6 Closed Cell Glassware Motor Assembly Holds the motor that drives the fan blades Cell Cap Encloses the fan blade WARNING AA Always keep body parts hair jewelry and clothing away from the fan blades P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 21 Condenser Condenses and reclaims the solvent Includes an inlet and outlet for the coolant tap or chilled water and a drain for the reclaimed solvent WARNING AAAA The liquid exiting the solvent drains may be corrosive flammable and or toxic Refer to the MSDS Material Safety Data Sheet for detailed information Do not touch drainage Keep drainage from heat or flame Dispose of drainage and container properly Avoid direct contact with this liquid Periodically check drainage tubes for damage Make sure drain flow is not inhibited Only use waste containers large enough to hold the drainage Concentrator Tube Sample Tube Contains the sample to be concentrated Sample tubes for endpoint detection at either 1
30. aporation Lights indicate when each cell is evaporating or has reached the selected endpoint The Start Stop buttons enable you to stop the evaporation manually if desired The two displays on the front panel display the bath temperature and time elapsed Concentration TurboVap 500 Mrs Set Time Expired Sensor Endpoint Set Time After Sensor stop Pressed Temperature Time Left Cell m m Eg In Process Stopped Done Right Cell Start Stop In Process Stopped Done Fan Speed Figure 5 TurboVap 500 Control Panel P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 18 Concentrate Until Options Concentrate Until Button Used to select the desired endpoint for the concentration Set Time Expired to concentrate for the period of time in minutes specified by the Time setting on the panel Sensor Endpoint to concentrate until the sensor detects a level of 0 5 or 1 0mL depending upon the style of concentrator tube Set Time After Sensor to concentrate past the 0 5mL or 1 0mL setting by the amount of time in minutes specified by the Time setting Stop Pressed to concentrate until the operator presses the Start stop button for the individual cell Manual operation Temperature Pushwheel Sets the desired water bath temperature in degrees Centigrade WARNING AA DO NOT operate at temperatures greater than 60 C with the sensors in place See Remov
31. ater bath temperature just below the boiling point Collect the condensed solvent Baking the Glassware Method Remove the glassware from the TurboVap clean bake and then reassemble 1 Disassemble the glassware and remove the PTFE sleeves 2 Clean all glassware with appropriate techniques 3 Bake the Pyrex glassware at a temperature up to 450 C 4 Replace the PTFE sleeves and reassemble the glassware NOTE 1 Do NOT bake the PTFE seal or PTFE sleeves P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Replacing the Fuses Fuse requirements Maintaining the TurboVap 500 100 120VAC model 220 240 VAC model 1 10A T250V P N C39164 2 5A T250V P N C44412 WARNING 52 MAA Electrical shock hazard Disconnect the power supply before changing the fuses For continued fire protection replace fuses only with ones of the same type and rating 1 Turn OFF the AC power and unplug the power cord 2 Locate the Power Entry Port fuse holder on the side of the unit 220 240V units have two fuse holders in the power entry port Always replace both fuses at the same time 3 Use a flatblade screwdriver to remove the fuse holder see Figure 28 Fuse Holder O locked Fuse Holder unlocked Fuse Holder 7Er m 4 Fuse LI om Figure 28 Replacing the Fuses 4 Remove the fuse and replace it with one of the same type and rating 5 Replace the fuse holder s in the
32. b for Coolant Supply tubing 15ft 1 4 ID tubing for Coolant Supply Instructions Clear Bath Kit two plastic Water Bath Covers Power Cord s 220V models include two power cords one for EU and one for UK Auxiliary Rack Siphon Spare seals Reference Card and Solvent Warning Sheet User s Manual CD Other Required Items Condenser coolant supply the chiller or cold tap water Tools Needed To install the concentrator you will need Flatblade screwdriver Wire cutters or scissors Site Preparation Requirements You must have an appropriate location with power and water sources available as specified by these site preparation requirements before installing the TurboVap 500 Closed Cell Concentrator Space Dimensions of the concentrator P N C128100 Rev 02 Height 24 inches 60 9 cm Width 21 2 inches 53 8 cm Depth 12 inches 31 cm Weight 40 5 Ibs 18 5 Kg with the water bath empty TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 24 Work Area Flat level stable surface Power 100 120VAC model 220 240 VAC model 100 120VAC 10 220 240 VAC 10 50 60 Hz 50 60 Hz Fuses 100 120VAC model 220 240 VAC model 1 10A T250V P N C39164 2 5A T250V P N C44412 Condenser Water Supply 1 4 ID supply 1 4 ID drain Tap water or chilled water without antifreeze Chiller if used Chiller must be
33. been designed with safety as a foremost consideration These products are equipped with a non arcing fan solid state electronics and provisions for vapor collection Do not change the configuration of the TurboVap 500 ventilation system Please consult with Biotage if you have any questions or concerns regarding this subject WARNING AA TurboVap products are NOT classified as Explosion Proof The power switch is an arcing source and could ignite explosive vapors if present Contact Biotage 1 Point Support Biotage 1 Point Support provides expert services including telephone troubleshooting of products repair instructions service dispatching and replacement part information Before you call Check the online Help and the manual for a probable cause and solution to your question Have the following information available for the customer support representative Workstation serial number s If applicable the error displayed on the LCD display To reach the Biotage 1 Point Support US 1 800 446 4752 or US 1 pointsupport biotage com Europe and Asia Pacific Regions 46 18 56 57 11 or EU 1 pointsupport biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com Biotage Product Repair Depot The Biotage Product Repair Depot offers product repair services upgrades refurbishment and installation at reasonable costs and with quick turnaround for all customer owned equipment and accessories For further informat
34. cedure on page 67 Replace the motor if necessary Perform the Motor Test Procedure on page 67 Replace the motor if necessary Stops prematurely using Sensor Endpoint or Set Time After Sensor Sample darkens too rapidly Restart to establish a new baseline reading or use the Stop Pressed selection Air bubbles around sensor optics Perform the Procedure for Dislodging Bubbles on page 63 evaluate status of the concentration reset and restart Faulty or worn sensor Perform the Sensor Test Procedure see page 65 and replace sensor if necessary P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 60 Condition Probable Cause Solution Doesn t stop using Sensor Endpoint or Set Time After Sensor Sample concentrates too slowly or opaque sample coats the glass Use Set Time Expired or Stop Pressed selection or change conditions to speed concentration Faulty or worn sensor Perform Sensor Test Procedure see page 65 and replace sensor if necessary Turbid bath water Clean and use Clear Bath additive Concentration rate too slow Low water bath temperature Increase bath temperature Low fan speed Increase fan speed Fan blade loose or not installed Check fan blade Faulty motor Perform the Motor Test Procedure on page 67 Replace motor if necessary Concentration rate too fas
35. condenser and cell cap Glass Covers for 500mL sample tubes 2 ea C45412 Glassware Part Glassware Number Condenser C128136 Cell Caps 2 set C45210 Concentration Sample Tube 1 0mL stem 500mL 2 C128140 P N C128100 Rev ea recommended for best recoveries with semi volatiles 02 TurboVap 500 User s Manual Biotage AB Replacement Parts 71 Replacement Kits Spare Parts Part Glassware Number Concentration Sample Tube 0 5mL stem 500mL 2 C128137 eo for best recoveries with non volatiles Concentration Sample Tube flat bottom 500mL 2 C128141 ae for best recoveries with non volatiles Part Replacement Kits Number Electronics Cover Replacement Kit with keypad C103211 Control Board Replacement Kit C45711 Drain Tubing Replacement Kit C45709 Sensor Replacement Kit C45713 Motor Replacement Kit C46803 Condenser Tubing Replacement Kit C50197 Bath Replacement Kit C46985 Part Spare Parts Number Motor Cover C45224 Fitting coupling panel mount 1 8 C45350 inlet outlet couplings on unit Fitting coupling insert right angle 1 8 barb C45351 connects Condenser tubing to unit Fitting coupling right angle 1 4 barb C45352 connects 14 chiller tubing to unit Cap screw on connects drain tubing to C45355 Condenser Fan Assembly internal cooling fan C45362 Interconnect Board C45705 Drain Cap Insert 1 ea C45771 Ga
36. coolant temperature affects the evaporation rate coolant water should NOT contain antifreeze a stable system without temperature fluctuations is necessary to prevent freezing at the lowest coolant temperatures NOTE 0 Coolant temperature should always be greater than 4 C to avoid the possibility of FREEZING the coolant P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 38 Fan Speed Considerations When selecting the fan speed consider the following higher fan speed causes faster evaporation rates excessively high speed can cause loss of analyte due to splashing for semi volatile analytes best recoveries have been obtained at the lower fan speeds A or B 4000 to 5000 rpm Selecting the Fan Speed To establish the optimal fan speed setting run test samples at various speeds and compare the analyte recoveries Choose the fan speed that gives the best analyte recoveries Press the Fan Speed button until the light for the desired speed is lit each press advances to the next speed While the button is depressed the selection s speed appears in the Temperature and Time displays Selection Fan Speed rpm A 4000 B 5000 C 6000 D 7000 E 8000 The displays return to normal when the button is released P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 39 Selecting
37. el 18 troubleshooting 60 Biotage AB 9 Biotage Biotage AB 10430 Harris Oaks Blvd Suite C Charlotte NC 28269 TEL 1 704 654 4900 Toll Free 1 800 446 4752 FAX 1 704 654 4917 http www biotage com
38. ell position 4 Clean the glassware according to your laboratory practice P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 48 Maintaining the TurboVap 500 This section of the manual includes e Daily Maintenance on page 48 Routine Maintenance on page 49 e Water Bath Maintenance on page 50 e Glassware Maintenance on page 50 Replacing the Fuses on page 52 Cleaning the Water Bath on page 53 e Refilling the Water Bath on page 54 Removing and Replacing the Sensors on page 54 Long Term Storage on page 58 Daily Maintenance e During Power Up diagnostics confirm that all displays are operational e Check to ensure water bath is full and clean e Check that the waste container is not full Avoid spilling any solvents on the control panel Avoid spilling any solvents in the water bath Cleaning Panel To clean use a mild cleaning solution on a damp cloth Do not spray cleaner directly onto the control panel Exterior Clean exterior surfaces with a damp cloth and mild cleaning solution when necessary WARNING AAAA Exposure to liquids may cause bacterial or viral hazards Liquids can also be corrosive flammable and or toxic Use good laboratory operating procedures when dealing with liquids Refer to the MSDS Material Safety Data Sheet for detailed information Avoid direct contact with liquids Wear protective gloves and
39. enser consumables 49 glassware maintenance 50 holding cups 15 Ordering apa aka KG NGA RARE bd EN 70 DAS 45 64 0094 naa B nG gat a 21 Condenser coolant choosing temperature 37 specifications 68 Condenser In connecting 33 Condenser Out connecting 33 Container specifications 69 Coolant connecting asas 8 en e Re a 33 Biotage AB inlet and outlet 17 Coolant supply 32 CHOOSING uuu d acid aan ieee wa xs 37 connecting tubing lines 32 5 30 T EA E A ETE E E S E S 32 OU kmo s EE EAE er d x 33 required 23 D Daily maintenance 48 Dark samples choosing endpoint 39 troubleshooting 59 60 Diagnostics Gell rise AE gr 65 display fest sortes rato a4 64 motor test 67 pushwheel connection 64 sensor test 65 software version test 64 Dimensions 68 Dislodging bubbles 63 Display LOS biet ded duisi gig d 64 test on power up 35 troubleshooting 61 Distilled water Glo le le AA AA ero APR 34 refilling water bath 54 required 24 Done bs ve aure ui ed arae 19 46 Drain Tubing Connecting 2266s wees ERE 30 kit included 22 required 24 E Endpoint selections Sensor Endpo
40. eports a value for the motor current for that cell left or right Sensor Test Procedure You should test the sensors when performing routine maintenance when suggested by the diagnostic charts To test the sensors 1 Remove any tubes from the unit 2 While holding the Fan Speed button press the cell Start Stop button 3 Record the value reported in the Temperature display no tube 4 Place a clean empty tube in the position P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 66 5 Record the value reported in the Temperature display Empty tube 6 Repeat steps 2 5 for the other cell position 7 Press the Fan Speed button once to exit the diagnostic mode Expected Sensor Values When Then no tube is in place value should be between 10 and 40 empty tube is in place value should be higher by about 10 than the no tube value The table below describes the possible causes for incorrect values When Then the value is too high there are bubbles around the sensor optics or low or the water level in the bath is below the level of the sensor or the sensor may be improperly connected defective expired and must be replaced Perform the Procedure for Dislodging Bubbles on page 63 and check the water bath level and sensor connections After these conditions are ruled out replace the sensor P N C128100 Rev 02 TurboVa
41. equire that you also rinse the condenser glassware with the appropriate solvent The sections below describe some common methods of cleaning the glassware Methods of Cleaning the Glassware The method used for cleaning the glassware depends on your samples solvents and laboratory practices Use good laboratory practices appropriate for your site In many cases only the sample tubes need to be washed between runs Three options are described rinsing the glassware with solvent see page 51 evaporating solvent see page 51 baking washed glassware see page 51 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 51 Solvent Rinsing Method 1 Rinse the condenser cell cap and fan blade with an appropriate solvent 2 Place a beaker in the condenser holding cup 3 Holding the condenser over the beaker rinse the inside walls of the condenser using a squirt bottle of solvent 4 Hold the motor assembly over the beaker and rinse the inside of the cell cap and surface of the fan blade using the squirt bottle of solvent NOTE 1 Avoid dripping solvent into the water bath Methylene dichloride can quickly cause disintegration of the sensors Other solvents can also damage the sensors Solvent Evaporation Method Wash the inside surfaces with freshly distilled solvent 1 2 3 Add 100mL of pure solvent to the sample tube Set appropriate conditions for the solvent to evaporate w
42. er Set aside the holders bath cover and screws Condenser Holding Cups N cT q Les Bath Cover EN ectangular Slot fer Sensor Wires PU id Sensor Connectors interconnect Board Figure 29 Condenser Holding Cups For high temperature operation it is NOT necessary to disconnect the sensors from the interconnect PC board The sensors will not operate in air 4 f you are removing the sensors a Use the flatblade screwdriver to remove the rear service panel screws Set the screws and service panel aside b Unplug the two sensor connectors from their receptacles on the sensor interconnect board Note that the receptacles and connectors are numbered 1 and 2 If you are replacing a single sensor only disconnect and remove the one that needs to be replaced c Use wire cutters to carefully cut any ty wraps from the sensor wires P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 56 Disconnecting the Sensors from the Sensor Mounts 1 Gently lift the rack out of the bath allow the water to drain and place the rack upside down to access the sensors on the underside of the rack Remove the sensor holding screws and use a flatblade screwdriver to push both of the sensors out of their holders Store the screws in a safe place if you intend to replace the sensors at a later date DO NOT remove the sensors by pulling on the wires 5 7 Screw 7 1 of 2 Sensor Sensor mou
43. et temperature 4 If the condenser coolant supply connections are not already attached connect the coolant supply to the condenser see Connect the Coolant Tubing Lines on page 32 5 Connect the solvent drain hose and ensure that the solvent waste container has the capacity to hold the solvent drainage see Connect the Drain Solvent Reclamation Tubing on page 30 6 Turn on the coolant supply NOTE 0 If a chiller is used allow time for the chiller to reach operating temperature The glass condenser reaches coolant temperature quickly P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Operation 45 Adding the Sample and Assembling the Closed Cell 1 Support the concentrator tube in the rack provided and add the sample 2 Remove one of the plastic covers from the water bath and place the sample tube into the water bath 3 Grasp the entire condenser and motor assembly and carefully place the condenser on top of the sample tube to assemble the closed cell Repeat for the second sample NOTE 0 To minimize evaporation from the water bath cover the unused position if you are running only one sample Starting the Concentration To start concentrating the sample 1 Determine the concentration method desired and press the Concentrate Until button until the desired option is selected 2 If applicable set the Time pushwheel to the desired time 3 Press the Fan Speed button until the amber lig
44. etection and tubes with 1 0mL stems concentrate to a volume of 0 75mL There is a calibrated mark on the sample tube indicating a volume of 1 0mL if you want to reconstitute the sample to this volume Accuracy of 1 0mL stem and graduated glassware 1 0mL graduation 0 02 mL 5 10 and 50mL graduations 0 5 mL 100 and 200mL graduations 2 0 mL Time Range 1 99 min Fan Speed 5 settings 4000 5000 6000 7000 and 8000 rpm Solvent Reclamation 95 of solvent vapors under typical conditions P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap Replacement Parts 70 Replacement Parts Units Part Units Number TurboVap 500 110 120V includes 1 0mL stem C103202 tubes caps blades auxiliary rack and sleeve kit amp tool NOTE 1 0mL recommended for best recoveries with semi volatiles 0 5mL stem tubes for non volatiles are also available TurboVap 500 230V includes 1 0mL stem tubes C103203 caps blades auxiliary rack and sleeve kit amp tool NOTE 1 0mL recommended for best recoveries with semi volatiles 0 5mL stem tubes for non volatiles are also available Accessories Part Accessories Number Auxiliary Rack 2 position 500mL tubes C45400 Closed Cell Assembly Set 1 0mL stem C128142 includes 1 ea sample tube condenser and cell cap Closed Cell Assembly Set 0 5mL stem C128143 includes 1 ea sample tube
45. he Sensor Endpoint selection See Sensor Endpoint Considerations on page 40 Stop Pressed Concentrating Until the Start Stop Button is Pressed To operate the TurboVap 500 manually use the Stop Pressed option To set up a Stop Pressed concentration 1 Press the Concentrate Until button until Stop Pressed is selected 2 Press the Start Stop button for the cell position s Reading the Time Display during a Stop Pressed Concentration The Time display shows the actual elapsed time alternately for each cell L precedes the left cell value and r precedes the right cell value If the elapsed time exceeds 99 minutes the number display is replaced with P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 42 General Guidelines For Achieving the Best Recoveries Clean and bake the concentrator tubes according to the standard cleaning techniques for your method Adjust the concentration conditions as needed operate at a fan speed that will generate adequate pressure If the concentrator tubes are initially FULL start with a low fan speed and increase the speed as the volume in the tube decreases select a water bath temperature carefully For methylene chloride acceptable temperatures can range from 38 to 45 C depending upon the other conditions set Remove samples promptly once the endpoint is achieved Use the proper technique for removing the sa
46. ht indicates the desired speed 4 Press the Start Stop button for each cell position used Time Display During Operation While the concentrator runs the Time display shows the actual elapsed time alternately for each cell L precedes the left cell value and r precedes the right cell value The Time display shows information other than time when Operating until the sensor endpoint a display of SE is displayed instead of a time Operating for a set time after the sensor endpoint a display of SE is displayed until the endpoint is reached Once the sensor endpoint is reached the additional elapsed time for each cell is displayed manual operation exceeds a time of 99 minutes a display of is displayed instead of a time See Software Diagnostics on page 63 for other display characters and what they mean P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Operation 46 Fan Behavior During Operation When you are concentrating until either of the sensor endpoints the fan may slow down or stop and start several times as the meniscus of the sample approaches the sensor optics Stopping the Concentration When Then running manually Stop Press Start Stop button for the Pressed selection corresponding cell you need to interrupt or stop press Start Stop button for the the concentration process corresponding cell unconditionally This resets the time
47. ing and Replacing the Sensors on page 54 for operation at temperatures above 60 C Temperature Display Displays the actua water bath temperature NOTE The display blinks if the actual water bath temperature is 2 or more degrees different than the set temperature WARNING AA To avoid the risk of fire or burn injuries do NOT operate the TurboVap 500 without water in the bath Time Pushwheel Sets the desired time in minutes total concentration time or additional time after sensor endpoint detection Time Display Displays the time elapsed The display indicates the left or right cell by flashing L or r before the time elapsed display SE is displayed if sensor detection is being used P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 19 Cell Selections and Functions Cell Button and Lights Function Start Stop Button Used to start or stop concentration for each cell In Process Light On when the cell is performing concentration Stopped Light On when the Start Stop button is pressed during concentration Done Light On when the cell has reached its endpoint Fan Speed Selections and Functions Fan Speed Selection Function A through E to set the speed of the cell cap fans A 4000 rpm B 5000 rpm C 6000 rpm D 7000 rpm E 8000 rpm WARNING AA Always keep body parts hair jewelry
48. ing the Fan Speed button press the top endpoint selection button 2 Confirm that Temperature and Time display the pushwheel settings 3 Set each digit to all ten values the corresponding display should track the switch If the displays do not reflect the pushwheel settings the cables may not be connected in the right orientation or at the right receptacle IF setting the pushwheel affects the wrong side of the corresponding display then cables are plugged into the correct connector but in the wrong orientation Temperature Time t Set Figure 31 Wrong Orientation P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 65 IF setting the pushwheel affects the display for the other pushwheel then cables are plugged into the wrong connector Temperature Time Set Set Figure 32 Wrong Connector 4 Release the fan speed button and the Display Test will resume for 4 seconds Cell Diagnostics Any time after the Display Test has run even during operation cell diagnostics can be performed to test the sensors and the motors Enter the Cell diagnostics by pressing either the left cell or the right cell Start Stop button while holding in the Fan Speed button Fan Speed Cell Start Stop Cell diagnostics Time display Sensor value Temperature display Motor value The Temperature display reports a value for the brightness of the sensor optics for that cell left or right The Time display r
49. int 18 Set Time After Sensor 18 Set Time Expired 18 Stop Pressed 18 Endpoint selecting 39 Evaporation rate 37 38 Expected motor current values 67 Expected sensor values 66 Exterior cleaning 48 P N C128100 Rev 02 TurboVap 500 User s Manual Index 74 F KC PAA AA 16 behavior 46 troubleshooting 99 Fan blade assembling icm Rhea 26 cleaning es ESA cd 35 3 10 ox X Pe 51 installiriga43 dox aac i RA 27 Fan speed DUON pb pA KA P REOR RR ERE 19 considerations 38 el AA 38 selections 19 specifications 69 Filing the water bath 34 FTBBZIIIO Ls baro d ox AA 37 Front panel PANG uana ka bee ed dox Ee ee 17 troubleshooting 61 Front view 15 Fuse specifications 68 Fuses included 24 replacing aser br FERE RS 52 G Gas Vortex Shearing action 12 Glassware assembling scam emu 29 paking aia eee cde o es 51 GIBOIPIO aqoa qox d Re 3e e 50 maintenance 50 Ordering ei d Rae Rede Pe XE diced 70 supplied 22 Guidelines 42 H Hardware 15 closed cell 20 front panel 17 front view 15 rear vieW
50. ion or to obtain a quotation for services contact the Depot US 1 800 446 4752 or US 1 pointsupport biotage com Europe and Asia Pacific Regions 46 18 56 57 11 or EU 1 pointsupport biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Preface 5 Features of the Biotage Product Repair Depot Factory trained repair technicians Two week turnaround from receipt at Biotage Shipping Customers are responsible for shipments both to and from Biotage specifying the carrier and choice of service Return Policy To ensure a safe environment for all our technicians it is mandatory for each returned product to include our chemical questionnaire stating contact chemicals chemicals used during application and cleaning steps taken prior to shipment Processing Once the product is returned it is evaluated for necessary repairs The customer is contacted with an estimate and may choose to go ahead with the repair or decline service If service is denied a minimum evaluation charge may apply Upon completion of the repair a purchase order or appropriate means of payment is required before return shipment Service and Customer Support Plans FCC Biotage offers a full range of services to ensure your success From our original factory warranty through a comprehensive line of customer support plans Biotage AB offers you Field Service Engineers and In house S
51. ion that doesn t use the sensors Temperature display shows Lo Temperature display shows hi Bath temperature sensor shorted Bath temperature sensor open or unplugged Contact Biotage 1 Point Support see page 4 Contact Biotage 1 Point Support see page 4 Time display shows Elapsed time has exceeded 99 minutes using Stop Pressed selection Normal behavior Time display shows SE using Sensor Endpoint or Time After Sensor selection Normal behavior Endpoint selection and fan speed lights blinking and Time 00 Power failure occurred Evaluate the concentration status reset and restart Light out on panel Faulty LED Replace the electronics cover or contact Biotage 1 Point Support see page 4 Temperature or Time display does not work Faulty display board Replace the electronics cover or contact Biotage 1 Point Support see page 4 Temperature or Time pushwheel does not work or shows the wrong value in the display Faulty pushwheel switch Perform the Pushwheel Connection Test on page 64 If it is not a connection problem replace the electronics cover P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting the Unit To diagnose problems with the unit locate the description of the condition in the table below Troubleshooting 62 Condition Probable Cause Solution
52. juries do NOT operate the TurboVap 500 without water in the bath P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 37 Water Bath Temperature Considerations When selecting the water bath temperature consider the following Faster evaporation occurs as the water bath temperature is increased However highly volatile analytes can be lost if allowed to sit for extended periods of time in a warm bath e You can select a water bath temperature just below the boiling point of the solvent in the sample tube Ensure that the sample especially in the stem will not boil Boiling the solvent affects sensor operation and can risk loss of sample Atemperature setting of 00 turns off the heaters Setting the Water Bath Temperature 1 Setthe water bath temperature using the numeric pushwheel 2 Place a plastic cover over any empty water bath position 3 Allow the bath to come to temperature the display stops blinking before using the concentrator NOTE 0 As the bath warms up bubbles may form around the sensor If given enough time approximately 1 2 hr bubbles should dissipate In most cases sample tube insertion will free bubbles Condenser Coolant Temperature Considerations Coolant supply to the condenser may be either cold tap water or water that is supplied by a chiller When selecting the coolant supply and temperature consider the following the condenser
53. l Concentration Conditions Established for Your Sample After experimenting with the selections presented in this section you should have established your settings for the following concentration conditions Water Bath Temperature C Condenser Coolant Tap _ _ e Chilled Cd Temperature C Endpoint Selection Set Time Expired Time minutes Sensor Endpoint Set Time After Sensor Time minutes StopPressed FanSpeed Use this information when following the operating steps in the next section P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Operation 44 Operation This section describes how to operate the Biotage TurboVap 500 Closed Cell Concentrator using the following procedures 1 2 6 7 Preparing the unit see below Adding the sample and assembling the closed cell see page 45 Starting the concentration see page 45 Stopping the Concentration if running in manual mode or need to interrupt see page 46 Disassembling the closed cell see page 46 Removing the concentrated sample see page 47 Shutting down after operation see page 47 Preparing the Unit Prepare the concentrator by performing the following steps 1 Check the water level in the water bath It should be at least as high as the initial solvent level in the sample tube 2 Turn the TurboVap power ON 3 Select the water bath temperature The display blinks until the water reaches the s
54. ls as shown in Figure 10 Inner Seal mm Outer Seal Sp Figure 10 Assembling the PTFE Seals 2 Place the two seals into the top of each glass cell cap as shown in Figure 11 Seals je l EXT Glass Cell Cap Figure 11 Placing the PTFE Seals NOTE 0 You must remove these seals before you bake the glass cell caps 3 Loosely screw the cell cap and seals into the motor assembly 4 Install the fan blade into the motor assembly a Align the flat on the fan blade with the set screws in the motor coupling as shown in Figure 12 sal PN Figure 12 Installing the Fan Blade b Tighten the set screw with the 0 05 Allen wrench supplied P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 28 Add PTFE Sleeves to the Ground Glass Fittings The TurboVap 500 comes with four PTFE sleeves and a sleeve installation tool The sleeves are placed on the ground glass ends of the Cell Caps and the condensers The sleeves shown in Figure 13 eliminate vapor leaks providing a better seal during concentrations The sleeves also allow easier separation of the glassware Figure 13 PTFE Sleeves NOTE 0 You must remove the sleeves before you bake the glassware 1 Place the lower end of a cell cap onto the lip of the installation tool 2 Starting with the wider opening of the PTFE sleeve place the sleeve onto the tool as shown in Figure 14 Cell Cap Ground Glass Joint
55. mine Optimal Concentration Conditions 36 Determine Optimal Concentration Conditions You should determine the settings that will best concentrate your samples before operation You should determine the best Water bath temperature condenser coolant temperature fan speed endpoint selection Set up a concentrator tube with the typical volume of sample or solvent and some analytes of interest Then experiment with the various selections The more efficient the concentration the better the analyte recoveries Optimizing the water bath and coolant temperatures the endpoint and fan speed will yield the best results when using the TurboVap 500 unit To prepare the TurboVap 500 1 Turn the evaporator s power ON NOTE The unit should always be ON whenever it contains samples so that the fan can remove exhaust 2 Remove all tubes from the evaporator Water Bath Temperature Description Concentrator tubes are placed in one of two positions in the temperature controlled water bath Since the concentrator s high concentration rates eliminate the need for a high temperature water bath the bath operates over a mild temperature range of ambient to 90 C This eliminates hot spots and improves sample recovery for more volatile compounds The water bath also can be brought down below room temperature by using evaporative cooling Cooling ability varies with the concentration rate WARNING AA To avoid the risk of fire or burn in
56. mples from their concentrator tubes Achieving Excellent Recoveries with 1 0mL Sample Stem Tubes 1mL tubes can eliminate the need for traditional triple quantitative transfer techniques The final crude or cleaned up extract can be taken to 1mL directly in the TurboVap concentrator tube 1 P N C128100 Rev 02 Set the TurboVap 500 parameters as desired selecting the Sensor Endpoint option Working on the bench or in a fume hood add the sample to the TurboVap tubes Do not add samples to the tubes while the tubes are in the TurboVap Start the concentration When the alarm sounds remove the sample tube from the water bath Failure to remove the tube promptly may result in analyte loss With a Pasteur pipette add solvent to the 1mL line Take the entire 1mL sample into the Pasteur pipette and rinse the lower 25 of the TurboVap tube i e 2 inches above the cone shape on the cylinder wall two to three times as shown in Figure 27 on page 43 TurboVap 500 User s Manual Biotage AB Determine Optimal Concentration Conditions 43 Achieving Excellent Recoveries with 1 0mL Sample Stem Tubes Continued Rinse the lower 2596 of the tube Bring to a volume of 1 0 m Lj Sample Figure 27 Rinsing the Sample Tube 7 Solvent evaporation during this rinsing will have reduced the volume Add fresh solvent to bring the sample to 1mL Rinse the tube walls two more times and transfer the sample to an autosampler vial Fina
57. n 64 SENSONS sa NAN NA dey Sa A 65 software version 64 Time displays 2a d urn iol IR m ho 18 display during operation 45 exceeds 99 minutes 45 pushwheel 18 selecting unu cade mm Rr 39 testing pushwheel 64 troubleshooting pushwheel 61 Time Range specifications 69 Tools needed for installation 23 Troubleshooting 59 concentration 59 HISBION sac od eh ae oak de NA 61 general iau eee 62 DOWOL aac ox R REOR Um i URGES 62 preventive maintenance 49 Tubing clips installing 30 Turning OFF 47 Turning ON iuueni nine 34 U UNPACKING hac SR do E Jed d CCCo nd 22 V Vapors troubleshooting 60 Vent TeatUles i av aca reo RC RS 14 Ventilation source 31 Version test 64 Volatile compounds 36 VONGX ARE 12 P N C128100 Rev 02 TurboVap 500 User s Manual Index 77 W Washing sample tubes 50 Waste container selecting 30 Water bath 15 cleaning a kawa eee NAG CR COO d e 53 LINE Aa 34 maintenance 50 refilling a s uaa aka arg eu 54 selecting temperature 36 setting temperature 37 specifications 68 temperature considerations 37 Water bath temperature 13 display bakahan aa Labada 18 pushwhe
58. n 3 Test the sensors by performing the Sensor Test Procedure on page 65 Software Diagnostics The concentrator software provides diagnostic capabilities to test the function of hardware and check the software version The following diagnostics are provided Power Up Diagnostics to test the lights on the unit check the software version running the unit test the Time and Temperature pushwheel connections Cell Diagnostics to e test the sensors e test the motors P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 64 Power Up Diagnostics The Power Up diagnostics are available each time the power is turned on Within the first four seconds of turning on the unit the lights are tested and you can check the software version and the Time and Temperature pushwheel connections Display Test Within the first four seconds of turning power on to the unit a display lights are lit If any lights are out check to make sure all connectors are seated If condition persists order the Electronics Cover Replacement Kit See Replacement Parts on page 70 Software Version Press the Fan Speed button within the first four seconds to display the software version in the Temperature display Pushwheel Connection Test The Time and Temperature pushwheel connections should be tested after replacing the control board or electronics cover To test the pushwheel connections 1 While pressing and hold
59. nditions Established for Your Sample 43 orcum 44 Preparing the Unities ES 44 Adding the Sample and Assembling the Closed Cell 45 Starting the Concentration et RH ERR REX en E RE REM REN RR UEOU EAE 45 Stopping the Concentration 46 When the Endpoint is Reached 46 To Silence the Alarim iiieit mete Eod o LR odes p IR UR ERR kang 46 Disassembling the Closed Cell en nennen 46 Removing the Sample from the Tube eese nene 47 Turning KUALA NA AP tnt taie 47 Maintaining the TurboVap 500 KAN NNKNK ANN NANANA nnne 48 Daily MsintelidiGe nakamtan NN Pa titt Me NAAT d NANA 48 Routine Maintenance sise 49 Other Preventative Maintenance 49 Typical Yearly Consumables ere NA 49 SONSOFS Tama D eto eot tomus rice ANA a nd 49 Water Bath Maintenance cccccccc eee ccneecceeee ce eeeaeeecae essa essa rem enm ener nennen 50 Glassware Maintenance sise 50 Methods of Cleaning the Glassware 50 Replacing the FUSes eto NANANA esee voto pi ei vete PAA TSAA 52 Cleaning the Water Bath cec Descr nine pat d RIS AA 53 Refilling th Water Ball ANA re LAT DAGANG 54 Removing and Replacing the Sensors 54 Remove the Covers and Unplug the C
60. nt Figure 30 Removing the Sensor Cut the ty wraps and remove the sensor wires from the clips on the edge of the rack If you are removing the sensors for high temperature operation place the wires into the slot on the panel and hang the sensors over the rear panel of the unit The sensors will not operate in air If you are replacing the sensor remove the sensor from the unit Replace the Rack and Covers to Operate Without Sensors Continue with these steps if you plan to operate the TurboVap 500 with water bath temperatures at or above 60 C Otherwise follow the instructions for installing a new sensor on page 57 1 P N C128100 Rev 02 Replace the rack in the bath making sure the holes on the top of the rack align with the holes on the upper lip of the bath Position the black top plate over the rack making sure the cutout on the underside of the plate faces the back of the unit Secure the top plate with four Phillips screws Plug in the power cord and turn the unit on TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 57 Operating Requirements When Sensors Are Removed You must only use Set Time Expired or Stop Pressed endpoint selections when the sensors are removed Installing the Sensors To reinstall all of the sensors or to replace a single sensor follow the steps below 1 10 11 P N C128100 Rev 02 Follow the steps in Remove the Covers and Unplug the Connectors on page
61. onnectors 55 Replace the Rack and Covers to Operate Without Sensors 56 Operating Requirements When Sensors Are Removed ssss 57 Installing de SSNS OS arsen ags tt dede ended leidet aec M Rs poe basse dos 57 kong Term Storage aegre deat e ERRARE RETE needs 58 Iroubleshooftlflig uris dese ane oran Eae euge mex apa a ERE NEED A FCR een 59 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Table of Contents 11 Troubleshooting the Concentration Process ssseem 59 Troubleshooting the Panel Display uns 61 Troubleshooting the Unit i eerie rrr ee ne en eae in ee el acne ee Lena 62 Procedure Tor Dislodging Bubbles e in oe pote eren retia Ea but edo ra et ond es 63 AG CA 63 mo MBISEBIo ror e M 64 Cell D IAGNOS NES de 65 Motor Test Procedure erp ret hp Date ere o ri ruere ee ede 67 DPECITICATIONS de M 68 Replacement POTES cuoi ANA fecit ati d oat omms ined dubi Lc ETE 70 T rboVap UNIS sise anne ones sara Or exce tex ote eT yv ee vaL Te HON QE e FREUT cru n 70 ACCESSORIES ini MONT nr PA ee a en ere 70 CSS Cp EE 70 Replacement KitSies kua NA eee ses ie edente pes aere Pee An E RUE eR ge ts 71 Spare Parts stone rte GG eee a Ho Ga a 71 Miscellaneous Iters enda e St tete ito HEU AA Aa a 72 DI sce tes 73 P N C1
62. oving Bearings Ol derings x acce mures ess replacing Lu ac quac pu E ded dd ed Bench location Best recovelles sd ad dei tem Rx Bevaline Bubbles dislodging 4 3 bee dca ER weds troubleshooting Buzzer Capacity ea 2 3 54d 434 ber pe Cell cap assembling DOTE Lessard DAS c ait eat Cell diagnostics Cell selections Changing the fuses Chiller choosing temperature P N C128100 Rev 02 TurboVap 500 User s Manual Connecting mapa aata cd WR Eri 33 requirements 24 CLA PAY 44 Cla ak ide dore m aa epe ad doa 40 Cleaning baking glassware 51 exterior 48 glassware 50 Dane es re 48 rinsing with solvent 51 solvent evaporation 51 water bath 53 Clear Bath adding asa AA 34 cleaning the water bath 53 included 24 maintenance 50 ONOSIING duda isa 72 refilling water bath 54 troubleshooting 60 Closed Cell adding sample 45 assembling ss xw ha 29 disassembly ss ss ess x Y X dde 46 Ordering s paa AKA KA KKK CR RN 70 DaltSa ea a2 30d gk hoe nese ees 20 Concentrate Until options 18 Concentration conditions 36 final conditions 43 SUDO os oh RTC TT TT dus 45 SIOPPING sa ar mis ere 46 Concentrator tube parts 21 Cond
63. p 500 User s Manual Biotage AB Troubleshooting 67 Motor Test Procedure You should test the motors when performing routine maintenance when suggested by the diagnostic charts To test the motors 1 2 5 6 7 Select the Stop Pressed endpoint Press the Fan Speed button until 6000 C is selected and KEEP HOLDING THE FAN SPEED BUTTON IN while pressing a cell Start Stop button Release the Fan Speed button and press the cell s Start Stop button to start the fan Once the fan reaches a steady speed record the value reported in the Time display 6000 rpm Press the cell s Start Stop button to stop the concentration Repeat steps 2 5 for the other cell position Press the Fan Speed button once to exit the diagnostic mode Expected Motor Current Values When Then no concentration is in process value should be 0 or 1 a steady speed is reached at 4000rpm value should be between 2 and 6 5000rpm value should be between 3 and 7 6000rpm value should be between 4 and 8 7000rpm value should be between 4 and 9 8000rpm value should be between 5 and 10 The table below describes the possible causes for incorrect values When Then The value is above 15 Check for a rubbing shaft or other when the motor is running source of friction The value continues to Check that the fan shaft is free to climb or exceeds 30 turn and that the motor is plugged in correctly
64. pecialists who are dedicated to supporting your hardware software and application development needs US 1 800 446 4752 or US 1 pointsupport biotage com Europe and Asia Pacific Regions 46 18 56 57 11 or EU 1 pointsupport biotage com Japan 81 422 28 1233 or JP 1 Pointsupport biotage com Our programs can include such useful services as preventative maintenance diagnostic servicing performed on site by Biotage AB field service engineers e extended use of the Biotage AB 1 Point Support Center e automated remote troubleshooting Software updates after hour weekend and holiday support repair depot servicing parts labor and travel expense coverage e other customized services upon request This device complies with part 15 of the FCC United States Federal Communications Commission Rules Operation is subject to the following two conditions This device may not cause harmful interference and This device must accept any interference received including interference that may cause undesired operation P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Preface 6 CE This device complies with all CE rules and requirements NOTE 1 Changes or modifications to this equipment not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment REMARQUE 1 Tout changement ou modification apport cet instrument non express ment
65. s dans une pi ce mobile ps Hb P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Preface 7 Table 1 Important Symbols Continued Description Description Risk of puncture injury Risque de blessure par piq re Risk of eye injury wear safety glasses Risque de l sion oculaire porter des lunettes de s curit Risk of exposure to biohazards Risque d exposition biohazards Risk of fire Risque d incendie Risk of poison Risque d empoisonnement Risk of explosion Risque d explosion Hazardous fumes manations dangereuses Hot surface risk of burns Surface chaude risque de br lures Protective ground symbol Symbole de terre de protection Ground symbol Symbole de terre Fuse Fusible Alternating current Courant alternatif On supply Marche alimentation Off supply Arr t alimentation CE compliance mark Marque de conformit CE Signifies that the unit has passed safety tests for grounding power line transience and current leakage Signifie que l appareil a r ussi les tests de s curit pour la mise la terre le courant transitoire de ligne d alimentation et la perte de courant Ox mD epb P PpP bDP i P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Preface 8 Table 1 Important Symbols Continued Symbol Description Symbole Description Input
66. safety glasses Dispose of liquid and container properly Do not overfill containers P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Maintaining the TurboVap 500 49 Routine Maintenance The routine maintenance procedures listed below should be performed every one to three months or as necessary Empty clean and refill the water bath see Cleaning the Water Bath on page 53 e Perform Power Up Diagnostics and Cell Diagnostics see Software Diagnostics on page 63 Other Preventative Maintenance To protect the motors from possible corrosive and damaging fumes inspect and replace the bearings and seals at least once a year every 6 to 9 months if the unit is frequently used Typical Yearly Consumables Sample Tubes 2 tubes are included Additional tubes can be purchased in cases of 2 tubes Sensors 2 sensors are included Additional sensors can be purchased individually Auxiliary rack 1 included with the unit Additional racks can be purchased separately Condenser 2 condensers are included Additional condensers can be purchased individually Replace the condenser only if it is cracked or broken Sensors The sensors in the TurboVap 500 are optical sensors Sensors have a wear life of up to 1 year with average use and up to 6 to 9 months for labs operating two shifts daily Two sensors come installed and ready to operate To prolong the life of the sensors keep the water bath cle
67. sample recovery Multiple Sample Processing The concentrator has two tube positions This allows either one or two samples to be processed simultaneously Independent Operation Concentration for each cell can be independently started or stopped at any time Requires No Hood The vent tubing permits the unit to be placed on the bench rather than taking up valuable hood space Vapor Removal The solvent vapors are removed by the fans through the Vent tubing The vent tubing must be routed to a suitable outside ventilation system P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB TurboVap 500 Hardware 15 TurboVap 500 Hardware TurboVap 500 Front Mounting Bracket L7 Condenser Holding Cups Front Panel _ Inlet and i Outlet 7 Water Bath Power Switch Figure 2 TurboVap 500 Parts Mounting Bracket Holds the motor assembly when not in use Front Panel Controls the setup and operation of the concentrator See Front Panel on page 17 for details Power Switch Turns the power ON or OFF WARNING AA The power switch is an arcing source and could ignite excessive explosive vapors if present DO NOT change the position of the unit s on off power switch when explosive vapors are present Condenser Holding Cups Hold the condensers when not is use Water Bath Warms the sample tubes during concentrations WARNING AA Fire and burn hazard Do NOT operate the TurboVap
68. sket on fitting in screw cap which to Condenser C45776 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Replacement Parts 72 Part Spare Parts Number PTFE Sleeve Kit 4 pkg C46431 for ground glass joints requires 46433 PTFE Sleeve Installation Tool required for 46431 C46433 PTFE Washer 0 45 OD x 0 25 ID x 0 02 THK C46686 in PTFE seal placed in glass Cell Cap Fan Blades and Shaft 2 set C46801 PTFE Seals for Fan Shaft 10 ea pkg C46802 Bearing 0 6 OD x 0 25 ID x 0 2 ED 0 69 diameter C47568 in motor mount Mount for the Motor and Bearing C47569 mount only no bearings or hardware Miscellaneous Items Part Miscellaneous Items Number Water Bath Additive Clear Bath 8 oz bottle C44316 Bath Siphon C46381 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Index Numerics 0 5 mL sample tube 1 0 mL sample tube removing sample A Accessories AGCCUMACY Ld uL doce piu dei rere d Adding sample Alarm behavior Alarm volume control Antifreeze Assembling closed cell motor cell cap and fan blade Automated concentration features B Baking the glassware Baseline Bath capacity mes deserdieagen Bath cover rem
69. ssware Disconnect and empty any waste containers Remove the condenser holding cups and the top plate Empty the bath o a fF O N Wipe the bath walls with an appropriate cleaner Rinse and re siphon the water from the bath 7 Remove clean and replace the rack 8 Replace the condenser holding cups and the top plate 9 Wait until the inside is completely dry 10 When dry cover the unit to prevent dirt or dust accumulation P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting This section provides Charts for troubleshooting your concentrator Troubleshooting 59 e Procedures referenced in the Solution columns If the solution listed is to contact Biotage 1 Point Support see page 4 Troubleshooting the Concentration Process To diagnose problems related to the concentration process locate the description of the condition in the table below Condition Probable Cause Solution Fan slows down or keeps turning on and off near the end of concentration using Sensor Endpoint or Set Time After Sensor Meniscus of sample approaches the sensor optics Normal behavior Fan keeps turning on and off and the tube is still full Air bubbles around the sensor optics Perform Procedure for Dislodging Bubbles on page 63 evaluate status of the concentration reset and restart Fan noisy or sounds slow Faulty motor Faulty motor Perform the Motor Test Pro
70. stilled water into the bath 2 Add 15 drops of Clear Bath additive 3 Add distilled water until the water is at the recommended height WARNING AA Fire and burn hazard Do not operate the TurboVap 500 without water in the bath 4 Place the plastic water bath covers over both of the water bath positions Connect the Power Cord 1 Plug the power cord securely into the receptacle on the back of the unit Make sure the plug is fully inserted 2 Connect the 3 pronged end to an appropriate power source WARNING AAA To avoid the risk of fire or electrical shock plug the power cord into a properly grounded outlet Turn ON the Power To turn on the concentrator turn ON the switch located on the lower left side of the TurboVap When the power is turned on the Power Up diagnostics described below will run automatically Note Upon power up the Start Stop button for the six cells will not work until an endpoint and fan speed have been selected P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Installing the TurboVap 500 35 Power Up Diagnostics When power is first turned on the following diagnostics occur Display Test During the first four seconds after power up all display lights are lit Confirm that no lights are out Normal operation begins after four seconds See Software Diagnostics on page 63 for other Power Up diagnostics P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Deter
71. t High water bath temperature Decrease bath temperature High fan speed Decrease fan speed Sample splashing High fan speed Decrease fan speed Low recovery of sample Sample boiling high bath temperature Decrease bath temperature Sample left too long after completion of concentration At the end of concentration remove the sample promptly Sample splashing high fan speed Decrease fan speed Improper sample removal Reconstitute to 1 0mL and use this volume to rinse the angled part of the tube and stem several times Leaking vapors Worn seal ground glass condenser joints require PTFE sleeves See Add PTFE Sleeves to the Ground Glass Fittings on page 28 P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Troubleshooting 61 Troubleshooting the Panel Display To diagnose unexpected behavior of the panel display locate the description of the condition in the table below Condition Probable Cause Solution Temperature display blinking Temperature not in range Allow enough time to come to temperature Contact Biotage 1 Point Support if continues Temperature display shows St unit beeps rapidly and bath heater turns off Temperature setting exceeds maximum allowed when the sensors are installed in the unit 60 C Decrease temperature setting OR remove sensors and use an endpoint select
72. the Endpoint and Time Settings There are four concentration methods concentrating for a set time in minutes concentrating until the sensor detects a meniscus concentrating for a set time after the sensor detects a meniscus concentrating until the operator presses Start Stop manual mode The table below describes when you should use each method and the sections that follow describe how to use each method When to Use Each Method The following table gives some examples of when you would choose a particular endpoint selection Use When Set Time Expired the sensors are removed because water bath temperature is greater than 60 C sample coats the glass or darkens rapidly sample evaporates too slowly Sensor Endpoint Set Time After Sensor your extracts are either opaque or slowly darkening you want full automation of sample concentration to a fixed endpoint acceptable for most samples at a water bath temperature under 60 C You want to concentrate past the endpoint of the glassware 1 0mL or 0 5mL or to dryness acceptable for some samples at a water bath temperature under 60 C Stop Pressed water bath temperature is greater than 60 C so the sensors are removed sample coats the glass or darkens too rapidly you need to concentrate for a time longer than 99 minutes you want to restart with a shorter time than previously programmed
73. ve acids To reduce the risk of electric shock do not remove the cover No user serviceable parts are inside Refer to qualified service personnel if help is required Use this product only in the manner described in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired AVERTISSEMENTS AA Pour r duire le risque de choc lectrique ne pas retirer le couvercle Ce produit ne contient aucune pi ce pouvant tre r par e par l utilisateur Au besoin confier l appareil un r parateur qualifi Ce produit ne doit tre utilis que comme d crit dans ce manuel Si cet appareil est utilis d une mani re autre que celle sp cifi e par le fabricant la protection fournie par l appareil peut tre entrav e P N C128100 Rev 02 TurboVap 500 User s Manual Biotage AB Preface 4 Product Safety Warning The TurboVap 500 power switch is the same type of power switch used on most laboratory equipment such as gas chromatographs spectrophotometers liquid chromatographs and computers The TurboVap products are safely used in the laboratory when Good Laboratory Practices are followed as with any other lab equipment All fans are brushless motor fans and will not ignite vapors The TurboVap is not classified as Explosion Proof and its use is at the discretion and risk of the operator or laboratory supervisor manager Your TurboVap product has

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