4 APPLICATIONS - user`s manual
Contents
1. 2 Screw the head with its plunger on the spray bottle firmly 3 Allow about 1 or 2 minutes for complete dissolution and mixing of the chemicals stirring gently with a circular motion of your hand Do NOT shake the container upside down 2 APPLICATION INSTRUCTIONS Lighting conditions The BLUESTAR FORENSIC latent bloodstain reagent produces a very bright and long lasting blue chemiluminescence that does not require total obscurity to be visible However at high blood dilutions the investigation will be much easier and the risks of missing a clue much lower if the product is applied in total darkness Indoors Close all the windows block all outside light sources and turn off all the lights Outdoors Wait for night time and turn off all area lights in an urban environment If necessary screen off distant light sources or even a very bright moon and work facing away from parasite lights Wait for at least 5 minutes to allow your eyes to adjust to darkness Your pupils will dilate themselves and you will be able to better perceive the BLUESTAR FORENSIC reaction Vaporization In order to prevent biological contaminations of the revealed traces wear personal protective equipment safety goggles gloves dust respirator protective clothing Bluestar 2006 Page 2 Do not spray toward another person The BLUESTAR FORENSIC latent bloodstain reagent is designed to be vaporized from waist height in a fine mist Adju2. BLUESTAR FORENSIC Latent bloodstain reagent User s Manual BLUESTAR FORENSIC TABLETS BL FOR TABLETS Update 9 7 03 30 2006 BLUESTAR 16 Avenue de la Costa B P 246 Monte Carlo 98005 MONACO Tel 377 97 97 31 77 Fax 377 97 97 31 61 E mail info bluestar forensic com www bluestar forensic com Bluestar 2006 Page 1 1 MIXING INSTRUCTIONS Container content Each BLUESTAR FORENSIC TABLETS container holds 4 pairs of tablets to make 4 x 125 ml 4 x 4 fl oz of BLUESTAR FORENSIC chemiluminescent solution or 500 ml 16 fl oz if all pairs are used together Required items Prior to mixing the product you will need the following items distilled water spray bottle mister equipped with an adjustable spray nozzle Active life Best results are obtained when the product is used within 3 hours after mixing the tablets in water There is therefore no requirement to rush the investigation due to immediate product deterioration Covered area A 125 ml 4 fl oz dose is generally sufficient to investigate a 25 m 250 sq ft area to search a vehicle or an object clothes rug knife etc A 500 ml 16 fl oz dose is generally sufficient to investigate about a 100 m2 1 000 sq ft area Mixing procedure 1 Open the spray bottle add 125 ml 4 fl oz of distilled water Then add a pair of BLUESTAR FORENSIC tablets If you need more working solution use 125 ml 4 fl oz per pair of tablets
3. differ from those of the typical reaction on blood Typically false reactions are markedly dimmer and whiter False reactions due to chlorinated detergents are often interesting because they may reveal attempts to wash or clean bloodstains and to conceal a homicide 3 PHOTOGRAPHY Photography of latent blood prints developed with chemiluminescence is not fundamentally different from regular daylight photography The same four basic elements of photography subject lighting film sensitivity aperture opening and shutter speed interact in exactly the same way Photography of BLUESTAR FORENSIC detected blood prints is easy and produces excellent results Equipment The chemiluminescence produced by the reaction of the BLUESTAR FORENSIC latent bloodstain reagent to blood is bright enough that no special equipment is needed However relatively long exposures may be needed for maximum picture quality and a tripod and flexible cable release are highly recommended in order to maintain the camera motionless during the time of the exposure A 24 mm lens is recommended Fil No special film is required but it is advisable to use low light fast films in order to obtain acceptably short exposure times A film speed of ASA 400 is generally suitable Lighting conditions Total darkness is not required Natural low intensity diffused light is preferred Artificial light tungsten or fluorescent produces yellowish or greenish pictures A f
4. lash should be avoided Bluestar 2006 Page 3 Instructions to obtain good pictures k Set the camera on a tripod perpendicularly to the area to be photographed Disable the automatic flash and the autofocus mode if the camera has one Use a large lens aperture typically a f 2 8 f stop value Set the exposure time to B Focus the lens manually over a spot of light provided by a flashlight over the blood area oa F oO D Turn off all the lights but darkness should not be complete Pictures shot in dimmed light will allow you to view not only the trace but other details of the scene as well N Re spray the blood print to reactivate a bright chemiluminescence reaction 8 Shoot several pictures using different shutter speeds typically 30 seconds 4 COLLECTING BIOLOGICAL PRINTS Samples of the revealed biological prints for subsequent DNA analysis are collected using the same methods as for any kind of biological prints 5 STORAGE CLEANING amp DISPOSAL Storage The BLUESTAR FORENSIC TABLETS latent bloodstain reagent has a 3 year shelf life AFTER MANUFACTURING expiration date imprinted on each foil If you wish to use the product after this date we recommend that you perform a test to check the product performances Note The product is warranted for 2 years after the DATE OF PURCHASE DO NOT attempt to store the product AFTER MIXING the tablets with water The mixed product is an active chemical compo
5. st the spray nozzle to obtain the finest mist possible Very little of it is actually needed Over spraying does NOT result in improved blood detection and in case the DNA is only available in very low quantities over spraying might dilute it too much for collecting exploitable samples thus compromising its analysis Check how the product reacts by spraying BLUESTAR FORENSIC on a testing sample This test will also help you become familiar with the reaction on blood Spray lightly horizontally ahead of you at least 50 cm 2 away from the target in a side to side sweeping motion NOT pointing toward the ground Indoors Be attentive not to saturate walls and vertical surfaces in order not to create drippings as if you were spray painting Outdoors Pay attention to wind direction if any Do not spray into the wind but use it to carry a light cloud of product over the area to investigate Identifying false reactions When reacting to blood the BLUESTAR FORENSIC latent bloodstain reagent emits an intense light blue chemiluminescence in the 420 to 440 nanometer range However false reactions may occur due to the presence of certain household detergents chlorine some paints and varnishes copper certain iron metabolizing plants such as lichens thyme and some tree mosses and certain soils containing iron Such false reactions are easily identifiable by the trained technician because their color brightness and duration
6. und that oxidizes Inert gases are constantly released and will in time accumulate under pressure in a sealed container causing swelling and leaking Cleaning Since the BLUESTAR FORENSIC latent bloodstain reagent is designed to be used on presumed blood on crime scenes all precautions and regulations related to the biohazards of blood apply when cleaning Disposal Dispose of unused mix in a sink under running water Dispose of cleaning residues according to local state and federal regulations applying to the biohazards of blood 6 MATERIAL SAFETY DATA SHEETS Material safety data sheets MSDS for the BLUESTAR FORENSIC tablets and working solution are available in PDF format on our web site www bluestar forensic com gb download php Bluestar 2006 Page 4
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