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1300 Series B2 BSC - Operating Manual

1. With the up down arrows select the period of time the fluorescent lamps are to remain lit The cabinet need not be attended when Light Interval Timer is selected If the sash is closed the fluorescent lamps will turn off Opening the sash will reset the Interval Timer OK Light Maintenance OK A Y to choose OK to select MENU to escape Lamp Hourmeter 1 Light Hourmeter The light has operated 1000 hrs 5000 hrs left Change Light Life A Y to choose OK to select MENU to escape The life of the light is set at 6000 hrs 2 Change Light Life Reset Light Clock 3 Reset Light Clock The light has operated 00 hrs MENU The fluorescent light intensity will wane with time This maintenance feature is a convenient reminder to replace the light periodically Reset the clock when the light is replaced A reminder will appear on the display when the set Light Life is reached 29 Operating Instructions Safety Cabinet 1300 Series B2 Thermo 30 Blower Operation A Y to choose OK to select MENU to escape 1 Auto Start 2 Manual Touchpad 7 Blower Operation The Auto Start selection programs the blower to automatically turn on and off when the sash is opened and closed The manual touchpad selection operates the blower through the control panel switch User Preferences Units of Measure 8 Cabinet Set Up 1 Units of Measure A
2. Every second optional RS 232 36 Calibration This selection is for the use of qualified certifiers only to adjust the blower speed or the display of the filter life remaining gauge Thermo Operating Instructions Safety Cabinet 1300 Series B2 Diagnostic Test Operation When the Diagnostic Test menu is selected the LCD allows keypad hardware testing to help troubleshoot any malfunction Keypad Testing When keypad testing is selected the LCD shows when each key is pressed to confirm the touchpad is working In addition when the Light and UV Light keys are pressed the associated relays are energized so the lamps should work The Timer button energizes an auxiliary relay When Mute OK is pressed the audio alarm should work When the A key is pressed the Airflow Alarm relay is energized When the W key is pressed the Blower On relay will be energized Finally the sash position Switches can be checked in this mode as the LCD displays the sash position information that the controller is receiving If An Exhaust Error Activates The most common causes of an Exhaust Error are e Exhaust blower is turned off or has lost power e Excessive loading of the Exhaust HEPA filter e Leakage in the exhaust system e Sudden change in room pressure When an Exhaust Error is activated the 1300 Series B2 Cabinet can be reset by pressing the Blower button The unit will restart and run for 15 seconds and the
3. The electrical receptacle cover may be removed to facilitate surface decontamination Biosafety Cabinets should be decontaminated for any of the following reasons Before maintenance work requiring entry into contaminated areas Before exhaust HEPA filter changes Before performing certification tests requiring entry into contaminated areas Before relocating the cabinet Before changing research programs After the gross spill of biohazardous material or toxic chemicals Thermo Operating Instructions Safety Cabinet 1300 Series B2 Correct Use The biosafety cabinet is a laboratory device for installation and operation in microbiological and biotechnical laboratories of safety levels 1 2 and 3 It has been designed as a Class ll safety cabinet Type B2 in accordance with NSF ANSI Standard 49 2004 Depending on the hazard level of the agents involved the operator must prepare in writing appropriate decontamination procedures for the device and the accessories used in the sample chamber Prior to the initial operation of the cabinet the operator must perform an installation test or certification According the NSF the test result must be documented by a test report The cabinet must only be used after it is in compliance with the operating parameters as specified by Thermo Fisher Scientific After any changes to the installation conditions and after any modification to the technical system the certification test must b
4. Wash hands and arms thoroughly with germicidal soap Wear a long sleeved lab coat with knit cuffs and over the cuff rubber gloves Use protective eyewear Wear a protective mask if appropriate Wipe Down Raise the sash to its full open position approximately 21 75 inches or 552 mm Mute the alarm by depressing the Alarm Silence switch Wipe down the interior surfaces of the cabinet with 7096 ethanol or a suitable disinfectant and allow to dry Loading Materials and Equipment Only load the materials required for the procedure Do not overload the cabinet Do not obstruct the front side or rear return air grilles Large objects should not be placed close together Slowly close the sash until it is in the correct operating position After loading the cabinet wait two to three minutes to purge airborne contaminants from the work area Work Techniques Keep all materials at least 4 inches 100 mm inside from the sash and perform all contaminated operations as far to the rear of the work area as possible Segregate all clean and contaminated materials in the work area Arrange materials to minimize the movement of contaminated materials into clean areas Keep all discarded contaminated material to the rear of the work area Avoid moving materials or the operator s hands and arms through the front access opening during use Avoid the use of an open flame Use disposable lab ware or an electric incinerator as alte
5. 33 UV Lamp Timer 34 Operating Instructions Safety Cabinet 1300 Series B2 Thermo The UV Lamp Timer allows you to decide whether the UV lamp will operate continuously or for a time period that you select Interval When the Operating Time option is selected the UV Operating Time menu is displayed UV Operating Time This window lets you set the amount of time the UV lamp stays lit when activated Use the A and V buttons to select the amount of time you need then press the OK button to set the interval NOTE Raising the sash will reset the timer to its original setting Pressing UV Light will turn the light off pressing Mute OK will pause the timer Continuous In the continuous mode the UV light will remain lit until the UV Light button is pressed or the sash is raised UV Lamp Maintenance This selection allows you to check how many hours the UV lamp has burned It also allows you to set the number of hours you want the lamp to last and to reset the UV lamp hourmeter UV Lamp Hourmeter This display only shows how many hours the UV lamp has been lit and how many hours remain until you will receive a warning to replace the lamp Set UV Lamp Life In this option you can set the number of operating hours before receiving the replace UV lamp warning For most UV lamps the output of UV light decreases at a constant rate Typically after 6 000 hours of operation the lamp will output 8096 of th
6. Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient overvoltages according to Installation Categories II Overvoltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 i e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 51 Operating Instructions Safety Cabinet 1300 Series B2 Note Accessories marked with an asterisk require a qualified certifier to install and or calibrate before use Telescoping Base Stands The user sets the height of these stands before installation The height can be set from 27 5 to 33 5 inches in 1 inch intervals providing a cabinet work surface height of 30 0 to 36 0 inches 1363 1364 1364 Catalog For use with Base Stand w Feet 1310 1311 1315 4 foot Thermo 1300 Series B2 BSC 3730402 1316 1317 1318 1360 1361 1362 6 foot Thermo 1300 Series B2 BSC 3730602 Seismic Bracket Kit 3857000 Stainless steel brackets an
7. and its effectiveness can be diminished or negated by dissolved proteins or metals or by other UV opaque substances protecting the target nucleic acid Because of its limitations UV light should be used as an adjunct to good surface disinfection practices In order to get optimum performance from the UV light it should be replaced after 6 000 hours of operation or less and the exterior surface of the lamp should be kept clean and free of dust Note The Cabinet records the number of hours of operation of the UV light You can program in the number of hours in 100 hour increments it will operate before a replacement message is displayed Note UV irradiation is absorbed by the tempered safety glass of the sash Independent research has shown that the level of UV irradiation on the outside of the cabinet s sash is equal to background radiation levels Note The UV sensitivity of a target organism varies depending on the UV output of the lamp the genus and species of the organism the medium the agent is suspended in etc Contact the facilities Health and Safety Officer at your facility for UV light use and recommendations Thermo Operating Instructions Safety Cabinet 1300 Series B2 Safety Precautions WARNING The Biosafety Cabinet should be certified by a certification technician before its initial use The cabinet should be recertified whenever it is relocated serviced or at least annually thereafter Do not assume that filter i
8. Flammable gases or solvents may reach explosive concentrations in the cabinet or ductwork If you feel that the procedure requires the use of an open flame or flammable materials contact the institution s safety office The use of air or gases under high pressure should be avoided as they may seriously disrupt the airflow patterns in the cabinet Thermo Operating Instructions Safety Cabinet 1300 Series B2 Now that the installation is properly prepared you are ready to inspect install and certify the Biosafety Cabinet This chapter covers how to e Unpack and move the Biosafety Cabinet e Install the cabinet e Connect the electrical supply source e Connect the service lines e Connect to an exhaust system Arrange certification of the Biosafety Cabinet Tools required for installation the Biosafety Cabinet include two 1 2 wrenches a flat blade screwdriver a 2 Phillips screwdriver and a carpenter s level Note The Biosafety Cabinet models weigh between 400 700 Ibs 182 318 kg The shipping pallet allows for lifting with a mechanical lift truck or floor jack If you must lift the product manually use at least six 6 persons and follow safe lifting guidelines Note The side panels must be removed to access the fasteners that secure the Biosafety Cabinet to the pallet DO NOT ATTEMPT TO LIFT THE BIOSAFETY CABINET BY THE SIDE PANELS DAMAGE WILL OCCUR Operating Instructions Safety Cabinet 1300 Series B2 Therm
9. Y to choose OK to select MENU to escape 1 Metric MPS 2 U S FPM Change Security 2 Security Lock A Y to choose OK to select MENU to escape 1 Non secure 2 Secure NOTE Please read section on Security Lock before selecting Secure This feature locks all programmed features and requires knowledge of an access code to unlock User Preferences cont OK Filter Life Display Options are fixed on the B2 model 3 Display Options Rate of Data Output 4 Data Output Rate A Y to choose OK to select MENU to escape 1 Every second 2 Every 10 seconds 3 Every 30 seconds 4 Every 60 seconds For data logging or monitoring through an RS 232 or RS 485 connection select the frequency data is downloaded See section or data logging for detail Restore Defaults 5 Restore Defaults OK to reset MENU to escape All data will be reset except calibration if OK is pressed See table of default settings if unsure Certifier Use Only 6 Calibration Enter password Only qualified certifiers should calibrate the cabinet s controls 7 Diagnostic Test OK See Diagnostic Test section in this manual Operating Instructions Safety Cabinet 1300 Series B2 31 32 Operating Instructions Safety Cabinet 1300 Series B2 Thermo User Selectable Features The Thermo Scientific 1300 Series B2 Bios
10. features please contact the Thermo Fisher Scientific technical support group at the phone numbers listed on the following pages Postal address USA Thermo Fisher Scientific Inc 401 Millcreek Road Marietta Ohio 45750 Enquiries from North America Latin America and Asia Pacific Phone 1 888 213 1790 Fax 1 740 373 4189 Email service led marietta thermofisher com Operating Instructions Safety Cabinet 1300 Series B2 Thermo Instruction of the Operating Personnel The operating instructions describe the safety cabinet 1300 Series B2 and applies to the models e 4 foot models 1310 1311 1315 1316 1317 1318 e 6 foot models 1360 1361 1362 1363 1364 1365 This safety cabinet has been manufactured in keeping with the latest technological developments and has been tested before delivery for its correct function It may however present potential hazards if it is not used according to the intended purpose or outside of operating parameters Therefore the following procedures must always be observed to prevent accidents e The safety cabinet should be operated by authorized personnel who have been trained on the use and maintenance of this product e For any operation of this device it is recommended that the operator prepares clear and concise written instructions in the language of the operating and cleaning personnel based on these operating instructions applicable safety data sheets plant hygiene guidelines an
11. final location adjust the height A plastic bag containing the mounting bolts for the Biosafety Cabinet is shipped with the base stand Remove and save it 2 Select the height of the stand and slide four 4 leg extensions into base stand corner posts and attach with 2 74 inch long bolt flatwasher lockwasher and nut Ensure that the same height hole is selected for each leg Tighten the leg bolts securely See Figure 3 1 Figure 3 1 Remove these four nuts and bolts to change the height of the base stand Operating Instructions Safety Cabinet 1300 Series B2 Thermo 3 CAUTION Only lift and support the cabinet at the stainless steel sides and back Damage will occur if lifted or supported on the front edge Move the base stand into its final location Using a carpenter s level adjust each leveling foot until the stand is level in both planes as shown in Figure 3 2 You are now ready to lift the Biosafety Cabinet onto its stand Figure 3 2 Level the base stand at these three positions Turn the four leveling feet to level the base stand to the floor 4 VU Connecting the Biosafety Cabinet to Utility Service Lines The service lines if any should be connected to the tube fitting s on the outside of the liner wall as shown in Figure 3 3 To install the tubing follow these steps 1 Ensure that the tubing is 74 inch O D soft metal and that the end has been completely deburred 2 R
12. provisions of the following directives 89 336 EEC 2004 108 EC 2006 95 EC when installed and operated in accordance with the manufacturers installation and operating instructions GL m Kavsas Cory C s n c 2 20 ark Schmitz M Place and date of issue Vice President Research and Engineering Labconco Corporation Labconco P N 3696065 Rev A 55
13. 1300 Series B2 Class Il Type B2 Biological Safety Cabinet Operating Instructions 3848304 Rev G Copyright These operating instructions are protected by copyright Rights resulting thereof particularly reprint photomechanical or digital postprocessing or reproduction even in part are only allowed with the written consent of the Thermo Fisher Scientific Inc This regulation does not apply to reproductions for in plant use Trademarks All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers Thermo Fisher Scientific Inc Controlled Environment Equipment 401 Millcreek Road P O Box 649 Marietta Ohio 45750 0649 United States Part 3848304 Rev G ECO H795 Thermo Operating Instructions Safety Cabinet 1300 Series B2 SCIENTIFIC CHAPTER 1 INTRODUCTION eren nnne nnn nnne nnn nnns 1 Instruction of the Operating Personnel seem 2 Waray EETCE 2 CHAPTER 2 PREREQUISITES eese nnne nnns 3 Space Requirements erit tee de EE ehe e te ree ve eda erue 3 Suggested Location iieri terere me cadets tee t cedes etre d usage Fd veu Ec ud pude 4 Exhaust Requirements seisnes ceci Eecec enc posent pt epo tee re Ree nit Eres nt gerere vigne 5 Electrical Requirements ssssssssssseeeseeeeneeneee nennen nnne ens 5 Service Line Requirements
14. HAUST CONNECTION IS A SEALED EXHAUST SYSTEM FROM THE TOP OF THE CABINET TO THE REMOTE BLOWER THE EXHAUST SYSTEMS SHOULD BE DEDICATED TO A SINGLE CABINET THE CABINET IS EQUIPPED WITH AN EXHAUST FLOW ALARM SUCH THAT AN ALARM SOUNDS AND THE CABINET BLOWER SHUTS OFF IN THE EVENT OF INSUFFICIENT EXHAUST FLOW If your research involves the use of toxic compounds or volatile materials contact your facility s safety officer or Thermo Fisher Scientific to ensure that your cabinet and its exhaust system are compatible with the materials you will be working with 11 Operating Instructions Safety Cabinet 1300 Series B2 Thermo Exhaust System Requirements The cabinet exhaust stack is sized to accept 10 inch 254 mm stainless steel ductwork The exhaust system s blower should be sized to handle the sum of 1 The pressure loss for the building s exhaust system duct work when it operates at the Cabinet s flow rate 2 PLUS The initial operating loss of the cabinet 1 0 inches 25 mm of water for the 1310 through 1318 Series B2 cabinets OR 1 4 inches 36 mm of water for the 1360 through 1365 Series B2 cabinets 3 PLUS In order to get the maximum possible life out of the cabinet s exhaust filter the system should be able to draw an additional 2 0 inches 51 mm of vacuum EXAMPLE A 1310 Series Cabinet is being connected to an exhaust system that has a calculated loss 0 5 inches of water when operating at the Cabinet s required flow r
15. IONS eene nnne nnne 51 Motor SpecifiCatiOns 2 t tenere er ee ree ee EE n RR REPRE Reo PER n ri 51 Environmental Conditions esesseeeen mener 51 APPENDIX D ACCESSORIES eese nnne nnne nnne 52 Telescoping Base Stands sssssesseeeene nennen 52 Seismic Bracket Kit ern eene eee e ete ire re ehe Te tbe ieee 52 Service Valve Kit 2 rite ain ed pe en petri deed edet vi ded eed ches 52 Ultraviolet arm piKits s 2 ei nort rne a eee ie Eee ez De ibat ete 52 IV Bar Kits Eee getestet d E es idee io rie E etes 52 Backdraft Damper EIEEE E E EA 52 ULPA Supply Filters eronneen 53 APPENDIX E QUICK CHART essen nnne nennen nnne 54 DECLARATION OF CONFORMITY eese nennen nnne nn nnn 55 Figure 2 1 Preferred location for the Biosafety Cabinet sssesssss 4 Figure 3 1 Adjusting height of the base stand sssse 9 Figure 3 2 Leveling the base stand ssss em 10 Figure 3 3 Connecting Biosafety Cabinet to service lines 11 Figure 3 4 Attaching drain assembly sese 13 Figure 4 1 HEPA filters 3 iiie cepe tiet enel eoe ie titus 16 Figure 4 2 Downflow Velocity Profile sssssssee 17 Figure 4 3 Inflow Volume or Average Inflow Velocity esses 18 Figure 4 4 Motor Blower recirculati
16. K to select MENU to escape UV Lamp Hourmeter The UV lamp has operated 01 Hours 5999 hrs left 1 UV Lamp Hourmeter Change UV Lamp Life 2 Change UV Lamp Life A Y to change OK to select MENU to escape The life of the UV lamp is 6000 hrs Reset UV Lamp Clock 3 Reset UV Lamp Clock The UV lamp has operated 00 hrs 4 Light Operation 5 Light Timer 6 Light Maintenance OK OK When the Interval Timer selection is confirmed the Light Interval Timer window will appear Operating Instructions Safety Cabinet 1300 Series B2 The effectiveness of a UV lamp decreases over its life and must be replaced periodically If your organization does not have established replacement standards typically it is suggested that UV lamps be replaced within 6 000 hours of use Lamp is approximately 80 of original intensity at 6 000 hours When the set time for UV lamp life is reached the user will be alerted with a message Replace UV Lamp Light Operation AY to choose OK to select MENU to escape 1 Auto Start P The Auto Start selection turns the 2 Manual Touchpad light on and off when the sash is opened and closed Manual selection places the control of the light through the touchpad control panel Light Lamp Timer Light Interval Timer AY to choose OK to A Y to choose OK to select MENU to escape select MENU to escape 1 Interval 2 Continuous 00 00 00
17. Select the secure option and press the OK button A lock icon appears on the LCD display Once engaged the operator must enter the proper sequence of keystrokes to operate the cabinet The proper sequence to unlock the Security Lock is Press the Y keypad 3 times within 2 seconds After successful entry of the unlock code the lock icon disappears from the display and the user has full access to the keypad functions To disable the Security Lock enter the Security Code submenu and select the non secure mode The lock is completely disabled Data Output Rate Note This selection will only work if the optional RS 232 board is installed This menu option selects the rate that the RS 232 board outputs data Data can be output at a rate of once per second once every 10 seconds once every 30 seconds or once per minute Restoring Software Default Settings To restore the default settings to the following configuration 1 Access the Menu and select User Preferences Press Mute OK 2 Inthe User Preferences submenu select Restore Defaults Press Mute OK 3 Press Mute OK again to restore the software defaults Pressing Menu will escape this submenu without restoring the defaults Table of Default Settings UV Lamp Auto Start Continuous Reset UV Lamp Hourmeter UV Lamp Life at 6000 hrs Blower Auto Start Units of Measure Ft Min Security Lock Non secure Data Output Rate
18. The touchpad of the 1300 Series B2 Cabinet is shown in Figure 5 3 Take a moment to get familiar with the buttons their location and function Also familiarize yourself with the display located on the right side wall The display will report system functions such as filter capacity timer displays alarm or error messages as well as icons that illuminate when cabinet functions such as UV light and blower are operational Blower Button Starts or stops the cabinet blower When the blower is in Auto Start mode closing the sash turns the blower off When the sash is raised the blower restarts automatically Pressing this button overrides the Auto Start operation Light Button Turns the fluorescent lamps on or off Closing the sash automatically turns the lights off When the lights are in Auto Start mode raising the sash turns the lights on automatically UV Light Button Turns the UV lamp on or off When the UV lamp is in Auto Start mode closing the sash turns the light on When the sash is raised the light turns off automatically Timer Button Allows you to select either a repeating interval timer or an elapsed timer stopwatch Mute OK Button Mutes all audible alarms for approximately 5 minutes unless itis a system error alarm When in the Menu mode this button is used to select an option Menu Button This button toggles the display between the display and menu modes When in the menu mode pressing this button r
19. afety Cabinet offers the user unparalleled flexibility and convenience The operation of the blower fluorescent and UV lights can either be operated manually or you may choose to use the Auto Start feature that activate functions automatically when the sash is opened or closed The UV lamp can be programmed to operate for a given time interval when the sash is closed before it shuts off NOTE During the first fifteen seconds of operation the Cabinet performs an auto diagnostic routine to check the operation of the microprocessor You cannot access the menu during this time NOTE When you are in the menu mode if a selection is not made within 30 seconds the display will reset back to display mode NOTE Pressing the appropriate touchpad button will override Auto Start selections To access the menu press the MENU button The display panel will show the first level menu To select from the various menu options press the A or Y buttons until the selected option is displayed Press OK to accept that option or press MENU to return to the previous menu level Timer Operation NOTE The timer button allows activation of an interval countdown or stopwatch elapsed timer The timers cannot be operated simultaneously To access the main timer menu press the Timer button anytime during normal operation The main timer menu is shown on the LCD display Use the A and Y buttons to highlight the Int
20. ate of 810 CFM What should the design capacity of the system be ANSWER 0 5 Exhaust system loss 1 0 Initial Cabinet loss 2 0 Reserve for maximum exhaust filter life 3 5 Vacuum total at maximum load Hot NOTE The exhaust system should be fitted with a backdraft damper part 3858800 to prevent the reversing of airflow in the system Drain Valve Installation In order to prevent damage during shipping the drain valve assembly has not been installed It is not required but if desired the valve should be installed after the Cabinet is in its final location To install the valve assembly follow these steps Note The work surface is heavy Use caution when handling it 1 Liftthe work surface out of the Biosafety Cabinet by lifting on the knobs at the front of the work surface Steady the work surface while pulling it straight out the front of the cabinet 2 Using a putty knife remove and discard the stainless steel cover that is sealed over the drain mounting holes Scrape out remaining sealant that is around the holes 3 Apply a light coating of silicone sealant user supplied to the mounting surface of the drain assembly Attach the drain assembly under the bottom of the cabinet as shown in Figure 3 4 Wipe off any excess sealant from the cabinet bottom Ensure that the center drain hole is unobstructed 4 Make sure the drain valve is in the closed position Reinstall the work surface 6 Allow the silicone sea
21. ciini etie dene ehu e eene Dn enden b De des 6 CHAPTER 3 GETTING STARTED eese nnne nnne nnns 7 Unpacking the Biosafety Cabinet ssseeee e 8 Moving the Cabinet 1 2 32 5 eire t merear e eei k ut oe 8 Preparing the Biosafety Cabinet for Operation sene 8 Installing the Biosafety Cabinet on an Existing Work Surface 8 Installing the Biosafety Cabinet on a Base Stand sse 9 Telescoping Base Stands sssssssseeeeene eene enne 9 Connecting the Biosafety Cabinet to Utility Service Lines 10 Exhaust System Connections ssessseeee enne 11 Exhaust System Requirements sssssseeeeeeneenenenn 12 Drain Valve Installation 22 1er tete ene Rc eene de Re Edere 12 Initial Certification ierit rtr nnne nte RR 13 HEPA FIlters itera nct ct cbe eter rte cene coe EHE Rcs etu Dee repe tad tas 15 Ul PAETOS iiti cer isto ete e ee e NS 15 L miriar Altflow 2 ier Her ids sdsnssdaedacsbat enerusccadercesdensenees 17 Directional Airflow nere eripe e leet repe tee eed 18 lere rd elec 19 Airflow SENSO e M 20 Cabinet Air Intakes Grilles Ductwork amp Air Balance Controls 20 Ultraviolet UV Lamp terere innere Deer tee e
22. d attaching hardware to secure Telescoping Base Stands in areas of seismic activity does not include fasteners to attach to floor Service Valve Kit 3747502 Includes serrated hose tip ball valve with quarter turn handle hardware and instructions for plumbing to services mounts on left or right side interior All cabinets are factory prepared to accept up to 4 fixtures Shipping weight 4 Ibs 2 kg Ultraviolet Lamp Kits field installed Includes a 254 nm UV lamp and ballast Catalog For use with Shipping Weight 3858510 All 115 VAC models 5 Ibs 2 3 kg 3858512 All 230 VAC models 5 Ibs 2 3 kg IV Bar Kits Bar supports intravenous solution bottles and bags Kits include IV bar mounting hardware and four hangers Catalog For use with Shipping Weight 3858611 4 foot Thermo 1300 Series B2 BSC 4 Ibs 1 8 kg 3858613 6 foot Thermo 1300 Series B2 BSC 6 Ibs 2 7 kg Backdraft Damper 3858800 The backdraft damper mounts in a vertical run of duct to prevent the reverse pressurization of the exhaust system Sized for 10 inch diameter ductwork Shipping weight 5 0 Ibs 2 3 kg Operating Instructions Safety Cabinet 1300 Series B2 ULPA Supply Filters Rated at 99 999 efficiency with particles 0 12 microns Catalog For use with 3859501 All 4 foot Thermo 1300 Series B2 BSC 3859503 All 6 foot Thermo 1300 Series B2 BSC 53 Operating Instructi
23. d eet etre eia 35 Reset Light Clock arr rre RE ERE ERR EEND 35 Blower Operation 5 omnei evi ee ee ebbe 35 User Preferences i rtu ee pe n e oett dote d eee cean 35 If An Exhaust Error Activates sseeee emen 37 If An Airflow Alert Activates esssseeeeeene emm emere 37 Resetting the Airflow Alert System sssssseee emen 37 Working in the Biosafety Cabinet sss 38 Operating Instructions Safety Cabinet 1300 Series B2 CHAPTER 6 MAINTAINING THE CABINET eene 40 Routine Maintenance Schedule essen 40 Service Operations er nete Dee idee deep E es 40 Work Surface Remowval sese enne eene 40 Front Grille RemowYVal 2 iret R aa AAE n tene AE u Hinter 40 Towel Catch Removal tiet de Lido eie rip e eed ttes 41 Front Panel Removal essen nemen nene nennen nennen 42 Changing the B2 Prefilter ssseee em 43 Changing the Fluorescent Lamps seee eem 43 Changing the Optional UV Lamp seeee me 43 Resetting a Circuit Breaker ssssssssssssssseeree enne 44 SLOFAGS EE EE E A TN TEE EAN E 44 CHAPTER 7 TROUBLESHOOTING eene nnns 45 APPENDIX A COMPONENTS eere enne 48 APPENDIX B DIMENSIONS eese nennen nennen nnn nennen 50 APPENDIX C SPECIFICAT
24. d technical regulations Most importantly e which decontamination measures are to be applied for the cabinet and accessories e which protective measures apply while specific agents are used e which measures are to be taken in the case of an accident e To ensure safety during the repair of the cabinet servicing of the equipment must be carried out by trained and authorized personnel Warranty Thermo Fisher Scientific warrants the operational safety and functions of the safety cabinet only under the condition that e the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions e the device is not modified e only original spare parts and accessories that have been approved by Thermo Fisher Scientific are used e inspections and preventative maintenance are performed at the specified intervals e aninstallation certification is performed prior to the initial operation of the device and a repeat certification is performed on the occasion of all inspections and repairs The warranty is valid from the date of delivery of the device to the operator Thermo Operating Instructions Safety Cabinet 1300 Series B2 SCIENTIFIC Before you install the Biosafety Cabinet you need to prepare the site for installation Carefully examine the location where you intend to install the cabinet You must be certain that the area is level and of solid construction In add
25. e UV light it did when it was new This option allows you to set the warning at a life you prefer in 100 hour increments Reset UV Lamp Clock This option lets you reset the UV hourmeter to 0 whenever the lamp has been replaced Light Operation This menu option allows you to select whether the fluorescent lights will turn on automatically every time the sash is opened NOTE The fluorescent lights will automatically turn off when the sash is closed no matter what its setting is Auto Start When the Auto Start option is selected the fluorescent lights will turn on every time the sash is opened Thermo Operating Instructions Safety Cabinet 1300 Series B2 Manual When the manual touchpad option is selected the fluorescent lights must be turned on and off at the touchpad Light Maintenance This selection allows you to check how many hours the fluorescent lamps have burned It also allows you to set the number of hours before replacement and to reset the light hourmeter Light Hourmeter This display only shows the hours the fluorescent lamps have been lit and how many hours remain until you receive a warning to replace the lamps Change Light Life In this option you can set the hourmeter before you get the replace light warning For most fluorescent lamps the output of light decreases at a constant rate Typically after 6 000 hours of operation the lamp will output less light than it did when it was new This option al
26. e of the prefilter if applicable Exhaust Error There is insufficient exhaust airflow for the cabinet to operate safely Pressing the Blower button will restart the blower If there is still insufficient airflow the alarm will sound again IF THE CABINET KEEPS GOING INTO EXHAUST ERROR DO NOT USE THE CABINET UNTIL THE PROBLEM HAS BEEN CORRECTED System Error The motor and display circuit board are not communicating properly DO NOT USE THE CABINET UNTIL THE PROBLEM HAS BEEN CORRECTED Performance Indicator The bar graph represents the capacity of the system When new filters are in the 1300 Series B2 Biosafety Cabinet the Performance Indicator should read approximately 10096 Over time the filters will load and reduce the available capacity The cabinet will continue to deliver the proper airflows while the Performance Indicator monitors the ability of the blower to overcome the filter loading Various factors such as temporary obstructions to airflow temperature and barometric pressure can cause the displayed percentage to vary slightly Ignore short term changes in the Performance Indicator s value As the Performance Indicator approaches 2096 remaining capacity consider having the cabinet filters replaced Icon Area The Icon Area displays icons for those features selected or in operation The Icons are Normal blower operation In this mode the cabinet blower is operating normally 25 26 Operating Instruction
27. e repeated and the test result must be documented in a way that demonstrates all operating parameters are in compliance with those specified by Thermo Fisher Scientific Incorrect Use To ensure optimum safety to the user and the environment the safety cabinet should not be used in laboratories that do not comply with the requirements of safety levels 1 2 or 3 The device must not be operated as a Class ll Type B2 safety cabinet if e no repeat test is performed after changes to the installation conditions or after modifications to the technical system e the alarm system of the device has issued a failure message and the cause for the failure has not been repaired The alarm system must not be tampered with or disabled If alarm system components have been removed or disabled for service or repairs the device must only be released for operation after all alarm system components are confirmed to be functioning properly The HEPA filters installed in this biosafety cabinet are designed to be excellent capture devices for particulate matter to a diameter of 0 3 um HEPA filters are not capable of separating gaseous substances Therefore avoid storing or processing gases or gas releasing substances in the device e which in quantity or concentration are toxic e ifareduction with other substances may result in hazardous toxic concentrations or formation of toxic gases e thatmay form combustive or explosive mixtures in combination w
28. ed and you will feel some resistance Note the orientation of the towel catch Also ensure that the DOP Sampling Line the clear tube with a black cap either passes through a hole in the towel catch or passes along the outside edge of the catch without being kinked 41 Operating Instructions Safety Cabinet 1300 Series B2 Thermo Maintaining the Cabinet Figure 6 2 Front Panel Removal Figure 6 3 1 Locate and remove the two Phillips screws that secure the front panel as shown in Figure 6 3 They are located on the top corners of the front dress panel 2 Liftthe front dress panel straight up and away from the cabinet 42 Thermo Operating Instructions Safety Cabinet 1300 Series B2 Changing the B2 Prefilter 1 Unplug the cabinet or turn off the System Reset Switch located on the top of the cabinet 2 Using an appropriate ladder or step loosen and remove the two wing nuts that secure the B2 prefilter in place The B2 prefilter is located to the left of the electronics module 3 Replace the B2 prefilter and retighten the wing nuts Changing the Fluorescent Lamps 1 Unplug the cabinet or turn off the System Reset Switch located on the top of the cabinet 2 Remove the front dress panel as noted in Figure 6 3 Remove the panel above the sash via four screws 3 Remove the fluorescent lamps by pulling the lamp sockets straight off each end of the lamp and releasing both lamps from the spring cli
29. edge and the exhaust outlet with plastic sheeting 2 Unplug the cabinet 3 Ensure that the cabinet will not be moved or disturbed while being stored Operating Instructions Safety Cabinet 1300 Series B2 Refer to the following table if the Biosafety Cabinet fails to operate properly If the suggested corrective actions do not solve the problem contact Thermo Fisher Scientific for additional assistance PROBLEM Cabinet blower and lights won t turn on Blower won t turn on but lights work Fluorescent light not working Fluorescent light is dim or flickering CAUSE Unit not plugged into outlet System Reset Switch is Off Circuit breaker s tripped Keypad disconnected or defective Sash closed Blower wiring is disconnected Blower motor is defective Keypad disconnected or defective Sash is closed Lamp s are defective Lamp wiring is disconnected Defective lamp ballasts Keypad disconnected or defective Lamp s are defective Lamp wiring is disconnected Defective lamp ballast CORRECTIVE ACTION Plug the Biosafety Cabinet into appropriate electrical service Check connection to control box on top of Cabinet Turn on the System Reset Switch Reset circuit breakers Run keypad diagnostics and check connections Raise sash Inspect blower wiring Replace blower motor Run keypad diagnostics and check connections Open sash Fluorescent lights will not wor
30. erval or Stopwatch Timer Press the OK button to select the highlighted timer function Thermo Operating Instructions Safety Cabinet 1300 Series B2 Interval Timer Operation 1 When selected the Interval Timer menu is displayed on the LCD The timer defaults to 0 00 00 hours minutes seconds Press and hold the A or V buttons to increase or decrease the timer interval When the proper interval is entered on the display press the OK button to start the timer When the timer reaches 0 00 00 an audible alarm will sound and the timer will reset itself and repeat the countdown Press the OK button to pause the timer Press the Menu button to clear the interval timer and return to the main timer menu Stopwatch Timer Operation 1 When selected the Stopwatch Timer menu is displayed on the LCD The timer defaults to 0 00 00 Press the OK button to start the timer Press the OK button again to zero the timer Press the Menu button to clear the stopwatch timer and return to the main timer menu Menu Options UV Lamp Operation This menu option allows you to select whether the UV light will turn on automatically every time the sash is completely closed Auto Start When the Auto Start option is selected the UV light will turn on every time the sash is closed Manual Operation When the Keypad Controlled option is selected the UV light must be turned on and off at the keypad
31. eturns you to the previous menu level Select Buttons Allow you to choose different options in the menu mode 27 28 Press MENU button to access Main Menu Main Menu A Y to choose OK to select MENU to escape 1 UV Lamp Operation 2 UV Lamp Timer 3 UV Maintenance Operating Instructions Safety Cabinet 1300 Series B2 Thermo Figure 5 4 Thermo B2 Menu Instructions NOTE Pressing OK button confirms selection Pressing MENU button returns to the previous Menu without changing the selection OK OK OK UV Lamp Operation AY to choose OK to select MENU to escape 1 Auto Start 2 Manual Touchpad Auto Start selection activates the UV lamp upon closing the sash UV Lamp Timer UV Interval Timer A Y to choose OK to A Y to choose OK to select MENU to escape select MENU to escape 1 Interval 00 00 00 2 Continuous When the Interval Timer selection is confirmed the UV Interval Timer window will appear With the up down arrows select the period of time the UV lamp is to remain lit upon closing the sash The cabinet need not be attended when UV Interval Timer is selected If the sash is opened the UV lamp will turn off Closing the sash will reset the Interval Timer WARNING It is important that all users be aware the Auto Start UV function is active so that samples are not inadvertently left in the cabinet exposed to UV UV Lamp Maintenance AY to choose O
32. f the Biosafety Cabinet If these instructions are missing or unclear contact Product Service at 1 888 213 1790 Installing the Biosafety Cabinet on an Existing Work Surface Note The Biosafety Cabinet is very top heavy Use caution when lifting or moving it When installing the Biosafety Cabinet onto an existing work surface or benchtop ensure that the structure can safely support the combined weight of the cabinet and any related equipment The work surface should be at least as wide as the Cabinet and 31 inches 787 mm deep to properly support the unit A hole or notch may be cut in the supporting surface in the right front corner to accommodate the drain valve Operating Instructions Safety Cabinet 1300 Series B2 Installing the Biosafety Cabinet on a Base Stand Thermo Scientific offers accessory Base Stands that effectively support the weight of the cabinet Stands for a B2 cabinet generally are not mobile since it is connected to an exhaust duct Telescoping Base Stands Adjust the leg height before installing the cabinet on top of the stand The height can be set in 1 inch intervals between 27 5 to 34 5 inches 699 to 876 mm providing a Cabinet work surface height from 30 0 to 37 0 inches 762 to 940 mm The Base Stands for each width Cabinet are listed in Table 3 1 below Table 3 1 Width Base Stand w Feet Model 4 3730402 6 3730602 1 Before positioning the Telescoping Base Stand in its
33. heck the prefilter and the air intake located below it to ensure that they are not blocked or restricted Ensure that roof blower is working Ensure that the exhaust damper is properly set by a qualified certifier Confirm that the roof mounted blower meets the volume and pressure requirements of the cabinet Inspect the exhaust system Inspect the exhaust system Inspect the exhaust system Replace the Exhaust HEPA filter Operating Instructions Safety Cabinet 1300 Series B2 PROBLEM Contamination of work in the cabinet CAUSE Improper technique or procedure for the Biosafety Cabinet Restriction of the return air slots or grille External factors are disrupting the cabinet airflow or the source of the contamination Cabinet is out of adjustment HEPA filter s are defective CORRECTIVE ACTION See Use of the Cabinet section in the manual Ensure that all return air slots grilles and the exhaust outlet are unobstructed See Installation section of this manual Have cabinet recertified 47 Operating Instructions Safety Cabinet 1300 Series B2 48 Illustration A 1 indicates the location of the following service parts and replacement accessory parts Biosafety Cabinet Replacement Parts Item Quantity Part No Description 1 1 3850500 Prefilter for 4 foot models 1A 1 3850501 Prefilter for 6 foot models 2 1 3838501 Exhaust HEPA Filter 4 f
34. ics module on the top right side of the cabinet The first two digits of the serial number are the year of manufacture the next two are the month The next 5 digits are the sequence of production and the letter following the serial number is the revision level of the cabinet Each motor must be programmed for the appropriate width cabinet Motors are not interchangeable As per ANSI NSF Std 49 with a background of 15 ft candles Measured in the geometric center of the work surface These values equal the Concurrent Balance Values CVB published by National Sanitation Foundation International NSF Thermo Operating Instructions Safety Cabinet 1300 Series B2 CE Declaration of Conformity We Thermo Fisher Scientific Inc 401 Mill Creek Road Marietta Ohio 45750 USA Ph 888 213 1790 Fax 740 373 4189 www thermo com being the representative party in the EU solely responsible for this certification hereby declare that the following product Equipment Laboratory Equipment Biological Safety Cabinets Type Model 1310 1311 1315 1316 1317 1318 4 Wide 8 Sash B2 Biological Safety Cabinet 1360 1361 1362 1363 1364 1365 6 Wide 8 Sash B2 Biological Safety Cabinet Manufacturer Manufactured for Thermo Fisher Scientific Inc by Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 USA is in conformity with the standards listed below EN61010 1 EN61326 1 EN55022 EN61000 3 2 3 following the
35. is on site testing is to be paid for by the customer Thermo Operating Instructions Safety Cabinet 1300 Series B2 All 1300 Series B2 Biosafety Cabinets operate using the following principles e Filtration and retention of particulates by High Efficiency Particulate Air HEPA filter s e laminar airflow e Directional airflow The major components in a Biosafety Cabinet are e The HEPA or optional ULPA filter s e The motor blower to force air through the Cabinet e Cabinet air intakes grilles ductwork and air balance controls HEPA Filters HEPA filters are disposable dry type particulate filters The filter material or media is typically made of borosilicate microfibers formed into a thin sheet in a process similar to the production of paper This sheet is folded or pleated to increase its surface area The pleats are held in place by aluminum diffusers or by beads of glue that add rigidity to the media pack The pack is then set into a frame and sealed as shown in Figure 4 1 The HEPA filter manufacturer establishes the efficiency of the filter by challenging it with an aerosol of known particle size The number of particles that penetrate the filter are quantified and this establishes the efficiency of the filter Thus the filters used in the Thermo Scientific 1300 Series B2 Cabinets are at least 99 99 efficient in removing particles 0 3 micron Note The HEPA filter media is very fragile DO NOT touch the media If you thi
36. ith air 23 24 Operating Instructions Safety Cabinet 1300 Series B2 Thermo System Reset Switch The Biosafety Cabinet has a system reset switch for resetting its microprocessors The switch is located on the front of the electronics module on top of the Cabinet as shown in Figure 5 1 Ensure that the switch is in the ON up position before attempting to operate the Cabinet Figure 5 1 The System Reset Switch Information Center The Information Center is an LCD display located on the right side wall at eye level The center s display is divided into three separate areas as shown in Figure 5 2 below The display will turn itself off one minute after turning off the blower or closing the sash Figure 5 2 Status Area 0 MU 1360 Series Be Performance Indicator i iB Bx ANEA Icon Area cy itm Ea fx Es s Measured Air Inflow iE Au Thermo Operating Instructions Safety Cabinet 1300 Series B2 Status Area The Status Area displays the system s condition during operation This line can display any of the following messages Sash is too high The sash is open too far for safe operation Please Wait Good practices should allow the blower to run at least three minutes to clean the air within the cabinet Airflow Alert The airflow patterns in the cabinet have changed resulting in a sudden change in the motor speed This is most likely due to a blockag
37. ition a dedicated source of electrical power must be located near the installation site Carefully read this chapter to learn e Location requirements e Electrical power requirements e Exhaust requirements e Service utility requirements e Space requirements Refer to Appendix C Specifications for complete biosafety cabinet electrical and environmental conditions specifications and requirements Space Requirements The dimensions for the different models are shown in Appendix B Dimensions Operating Instructions Safety Cabinet 1300 Series B2 Thermo RR d M Te LESTIE Suggested Location Note The Biosafety Cabinet should be located away from traffic patterns doors fans ventilation registers fume hoods and any other air handling devices that could disrupt its airflow patterns All windows in the room should remain closed Figure 2 1 shows the preferred location for the Biosafety Cabinet Preferred location Figure 2 1 Air register blocked or redirected to prevent cabinet disruption Alternate location Thermo Operating Instructions Safety Cabinet 1300 Series B2 Exhaust Requirements Before deciding on a location examine it carefully to ensure that it is compatible with the cabinet s exhaust duct The area directly above the cabinet s exhaust port should be clear of structural elements water and utility lines or other fixed obstructions There should be enough clearance to allow for the passage of a 10 PVC
38. k with the sash closed Replace defective lamp s Inspect lamp wiring Replace lamp ballasts Run keypad diagnostics and check connections Replace defective lamp s Inspect lamp wiring Replace lamp ballast 45 Operating Instructions Safety Cabinet 1300 Series B2 46 PROBLEM UV light not working UV light is dim or flickering Airflow Alert goes off and or there is a slight decrease in filter life remaining gauge Alarm sounds 15 seconds after the blower is started CAUSE Sash is open Lamp is defective Lamp wiring is disconnected Defective lamp ballast Keypad disconnected or defective Lamp is defective or is at end of operating lifetime Lamp wiring is disconnected Defective lamp ballast Supply HEPA filter loading Blockage of prefilter or its grille Roof blower is off Exhaust damper is improperly set Remote blower is improperly sized Mechanical failure of the exhaust system Obstruction in the exhaust system Leak or additional load on the exhaust system Loading of the exhaust HEPA filter CORRECTIVE ACTION Close sash UV light will not work with the sash open Replace defective lamp Inspect lamp wiring Replace lamp ballast Run keypad diagnostics and check connections Replace defective or worn out lamp Inspect lamp wiring Replace lamp ballast The gauge reading steadily decreases as the Cabinet is used C
39. lant to cure for at least eight hours before exposing it to liquid Thermo Operating Instructions Safety Cabinet 1300 Series B2 Figure 3 4 NOTE If desired the drain valve assembly attaches to the underside of the cabinet bottom Apply a light coat of silicone sealant to this surface of the connector aligning the three holes in the connector with the three holes in the Biosafety Cabinet liner Initial Certification Prior to use a qualified certifier should certify all Biosafety Cabinets Under normal operating conditions the Biosafety Cabinet should be recertified at least annually and when relocated or serviced The certifier should perform the following tests as recommended in ANSI NSF International Standard Number 49 in effect when the cabinet was manufactured e Downflow Velocity Profile Test e Inflow Velocity Test e Airflow Smoke Patterns e HEPA Filter Leak Test Operating Instructions Safety Cabinet 1300 Series B2 Thermo Site Installation Assessment Tests Vibration Test Noise Level Test Lighting Intensity Test These tests are user comfort related tests and may be omitted at the user s or certifier s discretion If you have any questions regarding certification agencies or need assistance in locating one contact Thermo Scientific at 1 888 213 1790 NOTE Service with costs Unless certification was expressly called for in the specification quotes and or purchase order the cost for th
40. lows you to set the warning at whatever level you prefer in 100 hour increments Reset Light Clock This option resets the light hourmeter to O after the fluorescent lamps have been replaced Blower Operation This selection allows you to choose to operate the blower in Auto Start or Keypad Controlled modes Auto Start When the Auto Start option is selected the blower will turn on every time the sash is opened When the sash is closed the blower will shut off Manual Touchpad When the Manual Touchpad option is selected the blower must be turned on and off at the touchpad User Preferences These selections allow for the customization of the display and the cabinet operation Units of Measure This option allows you to select the air velocity units of measure When Metric is selected the air velocities will be displayed in meters per second When US is selected the velocities will be displayed in feet per second Security Code NOTE The security code feature automatically reactivates and locks out the keypad every time the blower is shut off by the keypad or by closing the sash After the initial auto diagnostic screen is cleared the security lock reactivates 35 Operating Instructions Safety Cabinet 1300 Series B2 Thermo The security lock feature prevents operation of the Biosafety Cabinet by unauthorized users To engage the security lock access the Security Lock submenu as described in Figure 5 4
41. n will recheck the airflow If the airflow is still insufficient the unit will again show and Exhaust Error alarm NOTE If the Exhaust Error sounds during use take appropriate action to prevent contamination of you and others in the area NOTE If the cabinet keeps reporting Exhaust Error do not use the cabinet until the problem has been corrected If An Airflow Alert Activates The most common cause of an airflow alert is e Blockage of the B2 prefilter Note If the alarm sounds during use immediately take appropriate action to prevent contamination to you and other personnel in the area Resetting the Airflow Alert System The Airflow Alert automatically resets to normal operation once the motor speed has stabilized 37 38 Operating Instructions Safety Cabinet 1300 Series B2 Thermo Working In the Biosafety Cabinet Planning Start up Thoroughly understand procedures and equipment required before beginning work Arrange for minimal disruptions such as room traffic or entry into the room while the cabinet is in use Turn off UV light if included Slowly raise the sash until the bottom of the sash aligns with the sash indicator decal located on the left side of the work area Turn on the fluorescent light and cabinet blower if the Auto Start features have not been activated Check the air grilles for obstructions and note the filter gauge reading Allow the cabinet to operate unobstructed for 5 minutes
42. nk the media of a HEPA filter is damaged DO NOT USE THE CABINET Have the HEPA filter integrity tested by a certifier before using the cabinet Note HEPA Filters are only effective against particulate material Gases will pass through the filter ULPA Filters ULPA filters have the same properties as the HEPA filters described above except ULPA filters are rated at 99 999 efficient in removing particles 0 1 0 2 or 0 2 0 3 micron Operating Instructions Safety Cabinet 1300 Series B2 Thermo 4 Performance Features and Safety Precautions 16 Figure 4 1 Filter frame Polyurethane seal between media pack and filter frame Filter media pack A continuous sheet of filter media Thermo Operating Instructions Safety Cabinet 1300 Series B2 Laminar Airflow Laminar airflow is defined as the movement of a body of air in a single direction with a uniform velocity In practice the laminar downflow of air in the cabinet captures any aerosol generated in the work area of the cabinet and directs it to the HEPA filters In order to be true laminar downflow a number of individual downflow velocity test points The Downflow Velocity Profile must be 16 feet per minute 0 08 m s of the average of all the test points This is illustrated in Figure 4 2 Figure 4 2 17 Operating Instructions Safety Cabinet 1300 Series B2 Thermo 4 Performance Features and Safety Precautions Directional Airflow Direc
43. ns Safety Cabinet 1300 Series B2 Thermo The common service operations necessary to maintain the Biosafety Cabinet for peak performance are listed below Routine Maintenance Schedule Weekly e Using 70 ethanol or a suitable disinfectant surface disinfect the inside of the cabinet and the work surface e Using an appropriate glass cleaner to clean the sash and the surface of the UV lamp if so equipped e Operate the cabinet blower noting the percent filter life remaining in an operational log Monthly or more often as required e Using a damp cloth and clean the exterior surfaces of the cabinet particularly the front and top of the cabinet to remove any accumulated dust e Disinfect and lift the work surface Surface disinfect the lower plenum with a solution of 7096 ethanol or a suitable disinfectant Check the towel catch for retained materials e Check all service valves if so equipped for proper operation e Check the UV and fluorescent light hourmeters and record their readings in an operational log e Check the prefilter for cleanliness replace if needed e All weekly activities Semiannually or Annually e Have the cabinet re certified by a qualified certification technician e All monthly activities e Replace the B2 prefilter Service Operations Work Surface Removal Note The work surface of the cabinet must be thoroughly decontaminated before removing it 1 Liftthe front edge of the work surface
44. ntegrity and airflow performance have not been compromised during shipment Some internal components of the Biosafety Cabinet may become contaminated during operation of the unit Only experienced personnel competent in decontamination procedures should decontaminate the cabinet before servicing these components If you have any questions regarding certification agencies or need assistance in locating one contact Thermo Fisher Scientific Ensure that the Cabinet is connected to electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting the Biosafety Cabinet from electrical service Avoid the use of flammable gases or solvents in the Biosafety Cabinet Care must be taken to ensure against the concentration of flammable or explosive gases or vapors An open flame should NOT be used in the Biosafety Cabinet Open flames will disrupt airflow patterns in the cabinet burn the HEPA filter and or damage the filter s adhesive Gases under high pressure should not be used in the Biosafety Cabinet as they may disrupt its airflow patterns HEPA filters only remove particulate matter Operations generating volatile toxic chemicals or radionuclides must be evaluated carefully The media of HEPA filters is fragile and should not be touched Avoid puncturing either HEPA filter during installation or normal ope
45. o Unpacking the Biosafety Cabinet Carefully remove the outer carton and inspect the cabinet for damage that may have occurred in transit If the Biosafety Cabinet is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier Note United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Do not return goods without the prior authorization of Thermo Fisher Scientific Unauthorized returns will not be accepted If the cabinet was damaged in transit you must file a claim directly with the freight carrier Thermo Fisher Scientific is not responsible for shipping damages Do not discard the carton or packing material for the Biosafety Cabinet until all of the components have been checked installed and tested The cabinet is secured to the pallet in two places on each side To access the nuts and bolts remove the side panels by removing and keeping the two Phillips screws on both panels Swing the front of each panel away from the Cabinet and lift it straight up to remove the panel from the cabinet Moving the Cabinet Move the cabinet attached to its pallet by using a floor jack or a furniture dolly underneath the unit DO NOT move the cabinet by tilting it onto a hand truck Preparing the Biosafety Cabinet for Operation Installation instructions for the cabinet are attached to the sash o
46. on ssee 19 Figure 5 1 System reset switch emm 24 Figure 5 2 Information Center LCD ssssssssssseeenene 24 Figure 5 3 Control Keypad sse 27 Figure 5 4 Programming Menu Instructions eeee 28 Figure 6 1 Front grille removal sssssssssseeeneneeeener nennen 41 Figure 6 2 Towel catch removal ssssseeeeene eee enn 42 Figure 6 3 Front panel removal ssseee emm eee 42 Figure 6 4 Resetting a circuit breaker sssssssesssseeeeenneees 44 Figure A 1 Service parts replacement accessory parts sssssss 49 Figure B 1 Dimensions iine eene deporte tai ee qe adire e ieget 50 Thermo Operating Instructions Safety Cabinet 1300 Series B2 Dear User Congratulations on your purchase of a Thermo Scientific 1300 Series B2 Class Il Type B2 biosafety cabinet Your 1300 Series B2 biosafety cabinet has been tested and certified in accordance to NSF ANSI 49 and is designed to protect the user the environment and your research from harmful substances and cross contamination This user s manual provides instructions on how to use the 1300 Series B2 most effectively and safely The 1300 Series B2 offers a unique range of product features that will enhance your safety and improve overall operating efficiency Should you have any questions on using this product or need further explanation of any of its
47. ons Safety Cabinet 1300 Series B2 1310 1311 1360 1361 Model Series 1315 1316 1362 1363 1317 1318 1364 1364 Cabinet Size in feet 4 6 Sash Opening inches 8 8 Starting Serial 0705_ 0705_ Nominal Avg Downflow FPM 55 5 55 5 Nominal Average Inflow FPM 105 5 105 5 Nominal Avg Inflow Vol CFM 283 423 DIM Exhaust Volume CFM 665 998 Concurrent Balance Values s n less than 1304 Traverse Exhaust Volume CFM 810 1218 Differential Pressure in H20 1 9 2 2 Concurrent Balance Values s n 1304 and higher Traverse Exhaust Volume CFM 734 1191 Differential Pressure in H20 1 7 2 1 Supply HEPA Data P N 3838401 3838403 Exhaust HEPA Data s n less than 1304 P N 3838501 3838503 Exhaust HEPA Data s n 1304 and higher P N 3438501 3438503 Motor Blower Data s n less than 1304 P N 3832204 3832205 Motor HP A A Blower Size inches 10x8 10x8 Motor Blower Data s n 1304 and higher P N 3832209 3832210 Motor HP 72 2 Blower Size inches 10x8 10x8 Fluorescent UV Lamp Data Fluorescent Lamps 2 each F32T8 TL741 F40T8 SP41 Light intensity ft candles 90 100 90 100 UV Lamp G30T8 G30T8 UV energy uW cm 240 240 B2 Prefilter Data P N 3850500 3850501 54 The primary serial tag is on the data plate affixed to the light panel The secondary serial tag is located on the front of the electron
48. or stainless steel duct Avoid cabinet locations that require either an elbow directly on top of the cabinet s exhaust connection or an excessive number of elbows to clear other items For a further discussion of the cabinet s exhaust system requirements please go to Chapter 3 Getting Started Electrical Requirements The different Biosafety Cabinet models have the following electrical requirements Table 2 1 Model Requirements 1310 1311 115 VAC 60 Hz 12 Amps 1315 1316 1317 1318 230 VAC 50 60 Hz 6 Amps 1360 1361 115 VAC 60 Hz 12 Amps 1362 1363 1364 1365 230 VAC 50 60 Hz 6 Amps Note A dedicated outlet with a circuit breaker should be located as close as possible to the right rear side of the cabinet at a height even with or higher than the top of the bench or stand Operating Instructions Safety Cabinet 1300 Series B2 Thermo Service Line Requirements All utility service lines should be inch O D brass copper or stainless steel and equipped with an easily accessible shut off valve If the service line pressure exceeds 100 PSI it must be equipped with a pressure regulator to reduce the line pressure Consult local regulations for materials pressures and techniques regarding gas services Note The use of flammable gases or solvents should be avoided in the Biosafety Cabinet Open flame in the cabinet will disrupt the laminar airflow in the cabinet and may damage the HEPA filters
49. oute the tubing from the rear of the cabinet ensuring that it will line up with the slot in the back of the side panel The slot is located from 8 75 to 11 25 inches 222 to 288 mm from the bottom of the cabinet Note Make sure that the tube routing will not contact any electrical wires DO NOT loop service line tubing within the side panels of the Cabinet 3 Make sure that the nut on the tube fitting is loose but do not remove it Look inside the fitting to make sure the tube ferrule is there 4 Push the tube into the fitting until it is properly seated The tube will go approximately inch 19 mm into the fitting Thermo Operating Instructions Safety Cabinet 1300 Series B2 5 Tighten the tube fitting nut hand tight and then using a 7 16 inch wrench tighten it at least turn more 6 Close the service valve in the Biosafety Cabinet and then slowly open the shutoff valve on the service valve Test all fittings for leakage Tighten the tube nut slightly if needed Figure 3 3 Tube fitting nut 1 in D Tube fitting Seal fittings to cabinet with silicone sealant Exhaust System Connections The Total Exhaust Biosafety Cabinets are a Type B2 cabinet meaning they direct their HEPA filtered exhaust air out of the laboratory Note The B2 Biosafety Cabinet is designed to be connected to an appropriate exhaust system Do not attempt to operate it when it is not connected to an appropriate exhaust system THE EX
50. petente dre 20 Safety Precautions 2 einer net ie Ende RAE eSI AENEAS ahaaa dne 21 CHAPTER 5 USING THE CABINET eere 23 Correct USB beet cene esae dete ttr D eR RERO 23 In orrect USE m 23 System Reset Switch enean ederet tende Redde Pene EEEa a unen 24 Information Center iiie rece etre ie dites eine ai Ren ie cepe ies 24 otatus Area a1 eerte tn e ERE Boogie e MEE M HER 25 Performance Indicator 2 1 ice epe dre tee iere reet eese 25 oo Poe e N N a aa eA a 25 Operating the Sliding Sash ssesseeeene emm ene 26 Starting the Biosafety Cabinet ssssessseee enne 26 The Control Keypad esses eren nennen nennt nenn tens 27 User Selectable Featur s eiie eerie ees 32 Iudei EME 32 Interval Timer Operation oret rote titer reet Pru ien 33 Stopwatch Timer Operation ssssssseeeenene emen 33 Menu Options 33 UV Lamp Operations eint irte nint Here Ee re ARRAS teens 33 UV Eamp Tilmier s ceteri ie Dieter ei I Ue eee dole Dee Ede Enea 34 UV Lamp Maintenance sssseeeeeeen emen enne 34 Light Operations iiie ter aee tete ER rex ender 34 Light Maintenance 1 ecrit erret tee deine ede epe de dne de dle den 35 Light Fourmeter erede ie eee tiere DH vede Nae EAR 35 Change Lightlife vices 2 inii iden
51. ps that secure them in place 4 Install the new lamps by reversing the removal procedure Changing the Optional UV Lamp Note For optimum performance the UV lamp should be changed on an annual basis The UV lamp and the work area of the cabinet must be thoroughly decontaminated before removing the lamp 1 Start the cabinet and let it operate for 5 minutes 2 Raise the sash to its full open position 3 Thoroughly surface decontaminate the UV lamp and the work area of the cabinet 4 Unplug the cabinet or turn off the System Reset Switch located on the top of the cabinet 5 Remove the UV lamp by rotating it 90 degrees and lifting it straight up and out of its sockets 6 Install new lamp by reversing the removal procedure 43 44 Operating Instructions Safety Cabinet 1300 Series B2 Thermo Resetting a Circuit Breaker To reset any of the circuit breakers located on the left side of the electronics module depress the white button until it sets Figure 6 4 Circuit Breakers The front breaker protects the electrical outlets the rear breaker protects the motor and lights Storage If the Biosafety Cabinet is to be left unused for more than one month it should be prepared for storage Note The cabinet should not be stored in areas of excess humidity or temperature extremes If the cabinet is moved during storage it must be recertified before use 1 Close the sash completely and seal the bottom
52. ration If you suspect that a HEPA filter has been damaged DO NOT use the cabinet contact a local certification provider The HEPA filters in the Biosafety Cabinet will gradually accumulate airborne particulate matter from the room and from work performed in the cabinet The rate of accumulation will depend upon the cleanliness of the room air the operating time and the nature of work being done in the cabinet The Performance Indicator accurately displays the amount of filter life remaining Proper operation of the cabinet depends largely upon its location and the operator s work habits Consult the Installation and Normal Operation sections of this manual for further details Avoid direct exposure of plastic or coated materials to ultraviolet UV radiation Never bypass the UV safety interlock that only allows the UV light to work when the sash is closed 21 22 Operating Instructions Safety Cabinet 1300 Series B2 Thermo When surface disinfecting the Biosafety Cabinet Avoid splashing the disinfecting solution on skin or clothing Ensure adequate ventilation Carefully follow the disinfectant s safety instructions Always dispose of disinfecting solutions in accordance with local and national laws DO NOT allow disinfectants with high concentrations of free chlorine to contact the stainless steel components of the Biosafety Cabinet for a long period of time Free chlorine will corrode stainless steel after extended contact
53. rnatives Thermo Operating Instructions Safety Cabinet 1300 Series B2 e Use proper aseptic technique e Avoid using techniques or procedures that disrupt the airflow patterns of the cabinet e If there is a spill or splatter during use all objects in the cabinet should be surface decontaminated before removal Thoroughly disinfect the working area of the cabinet WHILE IT IS STILL IN OPERATION to prevent the release of contaminants from the cabinet Final Purging e Upon completion of work the cabinet should be allowed to operate for two to three minutes undisturbed to purge airborne contaminants from the work area Unloading Materials and Equipment e Objects in contact with contaminated material should be surface decontaminated before removal from the cabinet e All open trays or containers should be covered before being removed from the cabinet Wipe Down e Wipe down the interior surfaces of the cabinet with 70 ethanol or a suitable disinfectant and allow to dry e Periodically lift the work surface and wipe down the area beneath it e Inspect and clean the towel catch located at the rear of the work area beneath the work pan e Dispose of rubber gloves appropriately and have lab coat laundered properly e Wash hands and arms thoroughly with germicidal soap Shutdown e Lower the sash to turn off the fluorescent light and cabinet blower and activate the UV light if appropriate 39 40 Operating Instructio
54. s Safety Cabinet 1300 Series B2 Thermo lt 7 Fluorescent light In this mode the fluorescent lights are operating When the sash is fully closed the fluorescent lights automatically turn off Q UV light In this mode the UV germicidal light is operating When the sash is fully closed the UV light can be programmed to automatically turn on When the sash is raised the light automatically turns off to protect the laboratory from UV irradiation i Timer When either the interval or stopwatch timer mode is active this icon appears LA Security Lock When the security lock is activated the keypad does not respond to any buttons until the proper sequence of keystrokes is entered X Mute When this icon is on the audible alarm is muted for approximately 5 minutes Operating the Sliding Sash The counterbalanced anti racking sash mechanism requires only a few pounds of force to move the sash up or down You can open or close the sash smoothly with one or two hands positioned on either handle The sash position alarm and safety interlock system senses the sash position and acts appropriately The Biosafety Cabinet has been programmed to operate at an 8 inch 203 mm sash opening depending on model Raising the sash above its operating height will activate the audible and visual alarms The audible alarm can be temporarily muted for approximately five minutes by depressing and releasing the Mute OK button Closing the sa
55. sh back to its operating position will reset the alarm and defeat the muting of the alarm The safety interlock system senses when the sash is closed and allows the optional ultraviolet UV lamp to operate only when the sash is closed to protect the operator from irradiation Starting the Biosafety Cabinet 1 To start the Biosafety Cabinet raise the sash until its bottom edge aligns with the sash position label on the left corner post 2 Press the blower button to start the unit The unit will run a self test for approximately 15 seconds Additionally you will be instructed to Please Wait for three minutes to allow the cabinet to reach proper operating conditions If the display shows sash is too high and the alarm sounds recheck the sash position If the display shows exhaust error the cabinet blower will shut off and the audible alarm will sound Pressing the blower button again will restart the unit for 15 second after which the 1300 Series B2 Cabinet will again check the exhaust airflow IF THE CABINET KEEPS GOING INTO EXHAUST ERROR DO NOT USE THE CABINET UNTIL THE PROBLEM HAS BEEN CORRECTED Thermo Operating Instructions Safety Cabinet 1300 Series B2 3 To turn the UV light on the sash must be completely closed to prevent the escape of any UV radiation Push the UV light button to activate the UV light Note The sash must be completely closed for the UV light to activate Figure 5 3 The Control Keypad
56. straight up by grasping the knob handles at either front corner 2 Pullthe work surface straight out letting its rear edge rest on the center support underneath 3 Reinstall the work surface by resting the bottom on the center rail while pushing it back into the cabinet Be sure to engage the tabs on the back corners of the work surface with the slots on the rear wall of the work area Front Grille Removal Note The grille must be thoroughly decontaminated before removing it 1 Remove the work surface as described earlier 2 Atone end of the grille grip the front of grille with one hand and the back with the other hand Pivot that end of the grille upward and inward paralleling the angle of the sash as shown in Figure 6 1 Thermo Operating Instructions Safety Cabinet 1300 Series B2 Maintaining the Cabinet 3 Pullthe other end of the sash up and away from the bottom edge of the cabinet 4 Reinstall the grille by reversing the above sequence ensuring that the grille properly engages the bottom edge of the cabinet Figure 6 1 Towel Catch Removal Although not normally required the towel catch can be removed for cleaning inspection etc Note The work surface of the cabinet and the towel catch must be thoroughly decontaminated before removing either 1 Remove the work surface as described above 2 Remove the towel catch by pivoting the bottom out toward you as shown in Figure 6 2 it is spring load
57. t s n less than 1304 2A 1 3838503 Exhaust HEPA Filter 6 ft s n less than 1304 2B 1 3438501 Exhaust HEPA Filter 4 ft s n 1304 and higher 2C 1 3438503 Exhaust HEPA Filter 6 ft s n 1304 and higher 3 1 3832204 Motor Blower Assy 4 Ft TE s n less than 1304 3A 1 3832205 Motor Blower Assy 6 Ft TE s n less than 1304 3B 1 3832209 Motor Blower Assy 4 Ft TE s n 1304 and higher 3C 1 3832210 Motor Blower Assy 6 Ft TE s n 1304 and higher 4 1 3838401 Supply HEPA Filter 4 ft 4A 1 3838402 Supply HEPA Filter 6 ft 5 2 9721900 Lamp Fluorescent 4 ft 5A 2 9721903 Lamp Fluorescent 6 ft 6 1 1271300 Lamp UV models with UV light only 7 2 1287900 Receptacle Cover Operating Instructions Safety Cabinet 1300 Series B2 A 1 49 Operating Instructions Safety Cabinet 1300 Series B2 Thermo All dimensions in inches B 1 Width A p C 4 54 3 48 5 17 2 6 78 3 72 5 28 9 8 Working Height 21 7 MAX Thermo Operating Instructions Safety Cabinet 1300 Series B2 Motor Specifications Cabinet Model Electrical Requirements All Cabinets all Electronically Commutated Motor ECM Voltages 120 277 VAC 50 60 Hz 7 7 Full Load Amps 115VAC 4 3 Full Load Amps 230VAC 1 2 H P 1500 RPM maximum Automatic Thermal Protection Environmental Conditions Indoor use only
58. tional airflow also plays a key role in biosafety cabinet performance Air is drawn into the front of the cabinet at the front grille This curtain of air makes it more difficult for aerosols to escape out of the work area of the cabinet and into the outside environment This airflow is often calculated and referred to as the Inflow Volume or Average Inflow Velocity This is illustrated in Figure 4 3 Figure 4 3 Thermo Operating Instructions Safety Cabinet 1300 Series B2 4 Performance Features and Safety Precautions Motor Blower The motor blower assembly pulls air into the top of the cabinet and pushes air down through the supply HEPA filter which then flows down through the work area The external exhaust pulls the airflow into the front of the cabinet and from the supply filter through the exhaust filter and out of the building The motor in the Thermo Scientific 1300 Series B2 Cabinet is an electronically commutated motor ECM The ECM is a brushless DC motor that includes its own power supply to convert the incoming alternating current to direct current as well as its own microprocessor to control and measure the motor s operation The motor is preprogrammed to deliver a consistent volume of air even as the filter loads over time Figure 4 4 20 Operating Instructions Safety Cabinet 1300 Series B2 Thermo Airflow Sensor An airflow sensor located above the Exhaust HEPA filter constantly monitors the flow of exha
59. ust air out of the cabinet If the exhaust falls below a safe level the control board turns off the cabinet blower and sounds an audible and visual alarm This prevents the escape of hazardous material from the front of the cabinet in the event of an exhaust system failure The airflow sensor reading is shown on the display as Inflow in feet per minute or m s Cabinet Air Intakes Grilles Ductwork and Air Balance Controls The location size and pattern of the grilles at the front and rear of the work area affect cabinet containment and performance Note Never block or obstruct the grilles of the Biosafety Cabinet The internal ductwork of the Biosafety Cabinet conveys the air from the top of the cabinet to the blower and then from the blower to the supply filter The positive pressure rigid plenum of the Biosafety Cabinet is designed to deliver a more uniform airflow to the supply HEPA filter optimizing filter loading and operational life Ultraviolet UV Lamp The optional UV lamp generates a primary wavelength of light of 254nm A secondary emission is in the visible blue wavelength resulting in the characteristic blue color while operating UV light with a wavelength of 254 nm is biocidal primarily by creating thymine dimers in DNA These dimers prevent the correct transcription of the DNA into RNA resulting in cellular death or viral inactivation In order to be effective the UV light must directly strike the nucleic acid

1300 Series B2 BSC - Operating Manual

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