"user manual"
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1. aE EEEE 28 Maintenance 29 Excharge of Modules ast E E 29 biometra Manual T1 Thermocycler June 2009 11 Trouble sh0oling isein oraa 30 11 1 Slow heating and Cooling e tb geret p Ur t deb rrenen neonne 30 11 2 Restart due to unrecognized power failure eeeseeeseeeeeeeeee 30 11 8 Adaptation of protocols from other cyclers uusesensesseeennnnnnnennnnnnnnnnnnnnnnnnnnnnn nn 30 11 4 Recommendations for the use of 384 well Thermocyclers sssss 30 12 ADDEN d E 31 12 1 Technical specifications T1 Thermocycler 96 seeeseeeses 31 12 2 Ordering information td Eee een 31 13 Instructions for return shipment eoe eie io px He 32 14 VArreniby s 20 ceca tet teca EO ae tate dedu E cid we ICI Mea ence cree 33 19 Equipment Decontamination Certificate u44404444nnnannnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nn 34 16 EU Konformit tserkl rung EU Declaration of Conformity sssesssssss 35 17 Subjecr Index usa eee tuit av eate oed dx D ce E EE 36 biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 1 Introduction The T1 Thermocycler was designed to meet the highest standard of a today molecular biology laboratory The instrument combines the very successful Biometra silver bloc2. 10 3 7 Releasing wheel in case of blocked lid Note When the lid is in the extreme up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction To unlock wheel press metal pin with a ballpen and turn wheel carefully This pin overcomes the automatic clutch mechanism Thus care must be taken not to apply excessive pressure Lid arrested in upper position carefully turn wheel with pressed pin clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressure applied Lid arrested in down position carefully turn wheel with pressed pin counter clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid IMPORTANT WHEN THE CLUTCH MECHANISM IS ACTIVE OPTIMUM PRESSURE IS APPLIED NEVER USE PIN TO FURTHER INCREASE PRESSURE THIS WILL LEAD TO DAMAGE OF TUBES AND INSTRUMENT biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 4 Creating a Program 4 1 Select a directory In the T1 Thermocyler programs can be stored both in the main directory and in individual subdirectories For easy identification of the selected sto
3. Edit A list B C quit D enter Enter the number of the program you want to start Alternatively press A list to select a program from the list of all programs in this subdirectory Scroll through the list with cursor keys J Manual T1 Thermocycler June 2009 biometra a part of Analytik Jena 24 test 1 empty empty empty empty list B forward C quit D enter Pa UNHIO Confirm highlighted program with D enter The number and name of both subdirectory and the selected program will be displayed Directory 3 program no 0 Name TEST 1 preheating lid on start block signal on A list B B quit D start To start the program press D start 7 2 Display during operation During preheating of the lid the following screen is displayed Prog 0 TEST 1 dir 3 Step 1 temp C 25 0 C gt 65 0 time lid preheating A info B start stop C programs D Note In the default setting preheating on the block is held constantly at 25 C The program starts as soon as the lid has reached the set temperature During the preheating phase the display of the lid temperature alternates between the current lid temperature and preheating Prog 0 TEST 1 dir 3 Step 1 temp C 25 0 C gt 65 0 time lid preheating A info B start stop C programs D As soon as the block has reached the programmed hold temperature the timer starts to count down biometra
4. press D enter to open the programming spreadsheet 4 7 Enter temperature and time settings The programming spreadsheet Temp C time opt gt 1 2 3 4 A B insert delete C pgm OK D enter Note In this spreadsheet you can enter all parameters for your cycling protocol Furthermore you can set special parameters like touch down ramping rates or time increments For further details on these special parameters see section 6 Note You can navigate in the spreadsheet without limitations with the four cursor keys gt Note Each setting is confirmed with D enter The cursor moves automatically to the next field Alternatively you can confirm a value by moving forward with the cursor keys Note At any time you can call up the help function with A Note In an existing protocol hole program steps can be deleted and inserted For further information on deleting and inserting program steps see section 5 1 biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 15 Now enter temperature for the first step in the protocol Temp C time opt gt 1 65 0 2 3 4 A B insert delete C pgm OK D enter Confirm temperature with D enter or just move on with the cursor key If you move to the next row the setting will be stored in the temporary memory Temp C time opt gt 1 65 0 23 3 4 A B insert delete C pgm OK D ent
5. 0 Oh Om30s 3 80 0 Oh Om30s 4 35 0 Oh Om30s 2 29 5 4 0 pause A B insert delete C pgm OK D enter To hold the temperature for an indefinite time enter 0 After pressing D enter pause is displayed Note The minimum temperature you can set is 3 C 4 10Save program Save program with C pgm OK The program data are stored in the permanent memory Directory 3 program no 0 Name TEST 1 number of steps 5 runtime 1h10m23s edit A B e D The runtime is calculated and displayed Note To view the current remaining runtime at any time of the run press A info in the main menu biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 17 5 View edit program data You can check any existing program by scrolling through the editing mode with cursor keys mtl Select the desired program as described in section 4 2 Note During operation of the cycler the active program can be viewed but not modified If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory For further information about copying programs see section 5 2 5 1 Delete insert program steps The programming spreadsheet Temp C time e opt gt 2 65 0 Oh Om30s 3 80 0 Oh 0m30s 4 35 0 Oh Om30s 2 29 5 A B insert delete C pgm OK D enter To delete or insert a program step press B insert delete insert
6. The T1 Thermocyler checks the programs in all directories so called RAM check 3 5 The T1 Thermocycler display The T1 Thermocyler display provides information about the instrument and about the status of the active program prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D The individual function of the special keys A B C and D are specified in the display and depend on the context 3 6 Handling of the adjustable heated Lid To achieve optimum pressure on the tubes the T1 Thermocyler is equipped with an height adjustable heated lid smart lid technology Close the lid After the samples have been placed in the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel Note The pressure of the lid has been optimize for a fully loaded block If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure Open the heated lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then now you can open the lid with the knob Important The lid must not be opened under pressure because this leads to damage of the locking mechanism biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena
7. pause lid 98 8 C A info B start stop C programs D The program pauses in the current state The word pause is displayed and alternates with the elapsed time You can proceed with the program with B start stop biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 27 8 Special functions Main screen Prog step temp C 25 2 C time lid 25 4 C A B start stop C programs D To change special features of the T1 Thermocyler press D print signal language standard mode test mode A back B forward C quit D enter Uu e Ww NIAE Scroll though the list with the cursor keys ft Enter desired function with D enter 8 1 Print protocols 1 graphic protocol 2 graphic protocols 3 print program 4 print all programs A B C quit D enter Scroll through the list with the cursor keys t 9 Confirm the highlighted function with D enter 8 2 Switch beep on off There is an option for an audible signal whenever a program enters a pause and when the program is finished If this option is active the beep can be switched off by pressing any key of the keyboard with signal yes no A B C quit D enter In this window you can select whether there is a signal or whether there is not Using the cursor keys you can toggle between signal yes no Confirm your selection with D enter biometra Manual T1 Thermocycler June 2009 a part of Analy
8. use silicon oil Mineral oil may be used biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 3 First steps with the T1 Thermocyler 3 1 The T1 Thermocyler front View Wheel for the height Knob for release Se adjustement of the lid ofthe heated lid M DA n Keypad Graphical display Xx Power switch biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 3 2 The T1 Thermocyler rear view Fuse holder 1 N NA for 2 fuses for specifications of fuses Power cord see type label on the plug instrument s backside Manual T1 Thermocycler June 2009 Parallel printer port RS 232 port Type label including serial number specifications of fuses biometra a part of Analytik Jena 3 3 The T1 Thermocycler control panel Graphical display 7 Thermocycler 7 8 9 alfsife Power IN J Ls Easy to clean Ir PT olla keyboard backlit power on Power switch A B s gt Cursor keys For free navigation in the programming spreadsheet Use to scroll through lists Function keys the corresponding functions are specified in the screen biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 3 4 Initial self test After switching on the T1 Thermooyler the serial number of the instrument and the software version is displayed T1 Thermocycler Serial No 1234567 Vers 5 01t1
9. 30s 4 35 0 Oh 0m30s 2 A B header C pgm OK D enter Note In the main screen you will find an information that a special parameter has been modified This is indicated by a in the row labeled with opt gt 6 1 Program time increments To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the row labeled with dt s This value will be added to the time value from cycle to cycle Main funct dr ec dt s A s 2 5 00 3 5 00 4 10 5 00 5 5 00 A B header C pgm OK D enter biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 22 Note The time increment will have an impact on the total runtime depending on the numbers of cycles and the size of the increment A program with many cycles and large time increments will take a significantly longer time than a standard protocol 6 2 Program touch down For some applications it is useful to start with a higher temperature and to decrease the temperature from cycle to cycle This subsequent lowering of the temperature is called touch down To decrease a temperature from cycle to cycle enter a negative temperature increment in the row labeled with dT s Main funct dT c dt s A 9 s 2 5 00 3 5 00 4 0 20 5 00 5r 5 00 A B header C pgm OK D enter Note Be sure that the temperature decreas
10. 6 Ver 03 36 17 Subject Index BO GD ico nn 27 Declaration of Conformity 35 Decontamination sussssss 34 Heated Cid i aote 10 14 Heating and cooling rates 22 Language ae 28 Module Exchange ee ar 29 Program CODY reis een 18 Cr mE 11 Delete teet de 19 Increment sencer 21 Insert Delete step 17 biometra a part of Analytik Jena LOOP since este daba eost bak 15 METUENTES 12 PalSQ uci a PRAE RE ceed 25 SL 16 Sir ip OP P 23 SION Een 25 Protocol PUAN eR EE 27 Remaining run time 25 Safety precautions sssss 5 Solldast una 9 Technical specifications 31 Trouble shooting sssssss 30 Warranly steel 33 Manual T1 Thermocycler June 2009
11. TRA distributor Manual T1 Thermocycler June 2009 biometra a part of Analytik Jena 32 13 Instructions for return shipment In case of faults occurring with our T1 Thermocyler please proceed as follows Return only defective devices For technical problems which are not definitively recognizable as device faults please contact the Technical Service Department at Biometra Tel 49 551 5068640 Fax 49 551 5068666 e mail info biometra co uk Important Cleanse all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see 15 has to be attached to the outside of the packaging Use the original packing material If not available please use a comparable robust box Be sure that the instrument fits tightly into the box When packaging protect the device in a way to avoid scratches e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT Please enclose a note which contains the following a Senders name and address b Name of a contact person for further inquiries with telephone number c Des
12. a part of Analytik Jena Manual T1 Thermocycler June 2009 25 Prog 0 TEST 1 dir 3 Step 1 cycle 1 of 30 temp C 65 09C gt 65 0 time Oh 2mi5s gt 0h 5m Os lid 98 9 C A info B start stop C programs D The elapsed time versus programmed hold time is displayed 7 3 View remaining run time Display during operation Prog 0 TEST 1 dir 3 Step 1 cycle 1 of 30 temp C 80 0 gt 80 0 time Oh 0m23s gt 0h 5m Os lid 98 9 C A info B start stop C programs D Press A info to call up the current remaining time calculation Additional information about active pgm Status Step 2 Cycle 3 of 30 Remaining time 2h 57m Stop or pause key B in main menu A B B quit D The current remaining run time id displayed in line 4 7 4 Pause stop program Main display during operation Prog 0 TEST 1 dir 3 Step 1 cycle 8 of 30 temp C 35 0 C gt 35 0 time Oh Omi8s Oh 0m30s lid 98 8 C A info B start stop C programs D To pause or stop the active program press B start stop Stop pause directory 3 program no 0 test 1 A B pause C quit D stop Manual T1 Thermocycler June 2009 biometra a part of Analytik Jena 26 Select B pause to pause program D stop to stop program C quit to return to the program display When you select B pause the following screen is displayed Prog O TEST 1 dir 3 Step 1 cycle 8 of 30 temp C 35 09 gt 35 0 time
13. bi T1 Thermocycler 384 T1 Thermocycler in situ T1 plus Thermocycler 48 T1 plus Thermocycler 96 T1 plus Thermocycler Kombi T1 plus Thermocycler 384 T1 plus Thermocycler in situ T1 Modul 48 T1 Modul 96 T1 Modul Kombi T1 Modul 384 T1 Modul in situ types T1 Thermocycler 48 T1 Thermocycler 96 T1 Thermocycler Combi T1 Thermocycler 384 T1 Thermocycler in situ T1 plus Thermocycler 48 T1 plus Thermocycler 96 T1 plus Thermocycler Combi T1 plus Thermocycler 384 T1 plus Thermocycler in situ T1 module 48 T1 module 96 T1 module combi T1 module 384 T1 module in situ Best Nr Order No 050 900 050 901 050 902 050 903 050 904 050 910 050 911 050 912 050 918 050 914 050 920 050 921 050 922 050 923 050 924 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG und der EC low voltage directive 73 23 EEC and the EG Richtlinie ber die elektromagnetische Vertr glichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 55011 1998 A1 1999 A2 2000 EN 55022 1998 A1 2000 A2 2003 EN 61000 3 2 2000 EN 61000 3 3 1965 A1 2001 EN 61000 6 1 2001 EN 61010 1 2001 EN 61010 2 010 1994 A1 1996 Dr J rgen Otte Quality Manager biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena Formular 00
14. biometra a part of Analytik Jena Th Thermocycler User Manual June 2009 Ord No Model 050 900 T1 Thermocycler 48 050 901 T1 Thermocycler 96 050 902 T1 Thermocycler 384 050 903 T1 Thermocycler in situ 050 904 T1 Thermocycler combi Warning A Please read these instructions carefully before using this A apparatus Biometra GmbH Service Department Rudolf Wissell Str 30 Rudolf Wissell Strasse 14 16 D 37079 G ttingen D 37079 G ttingen Tel 49 551 50686 0 Tel 49 551 50881 10 12 Fax 49 551 50686 66 Fax 49 551 50881 11 email info biometra com email service biometra com Internet http www biometra com biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 N 9 10 a part of Analytik Jena Introduction parem ES 4 Before YOU SST RC 5 2 1 Safety PrOCAUMONS e en nel einander 5 First Steps with the T1 Thermoeyleraus u seen ee ren 6 3 1 The T1 Thermocyler front View u rte Ree De crepitu 6 3 2 The T1 Thermocyler rear view a essen 7 3 3 The T1 Thermocycler control panel uumssussssssnssnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnnnnannnnnnnn 8 3 4 TET prs zc 9 3 5 The T1 Triermooyeler display 5 tetuer a a 9 3 6 Handling of the adjustable heated Lid ee 9 3 7 Releasing wheel in case of blocked lid 10 CGreating a Prograf i nee teta US deme oe edunt e uite tcp Eger 11 4 1 Selecta direc
15. cription of the fault which also reveals during which procedures the fault occurred if possible biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 33 14 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the following conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra or an authorized distributor This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 34 15 Equipment Decontamination Certificate To enable us to comply with German law i e 828 StrlSchV 817 GefStoffV and 819 ChemG and to avoid exposure to hazardous materials during handling or repair will you please complete this form prior to the equipment leaving you
16. delete step 2 65 0 Oh Om30s 3 80 0 Oh Om30s 4 35 0 Oh Om30s 2 29 54 A B delete input C quit D enter Enter the number of the step that you want to insert delete Note Additional steps will be inserted before the selected step number The following steps will be shifted by one position insert delete step 3 2 65 0 Oh 0m30s 3 80 0 Oh 0m30s 4 35 0 Oh Om30S 2 29 5 A B delete input D enter C quit Note If you have entered a wrong number the field can be cleared with B delete input Confirm the step number with D enter Manual T1 Thermocycler June 2009 biometra a part of Analytik Jena 18 2 3 4 55 65 0 80 0 35 0 A insert insert delete step Oh Om30s Oh Om30s Oh Om30s 2 B delete 3 29 C quit D enter Press A insert to insert one step at the selected position Note Additional steps will be inserted before the selected step The following steps will be shifted by one position Press B delete to delete the selected step Note By deleting a step the following steps will be shifted by one position Note The insertion and deletion of steps may have an influence on existing cycles Be sure that all settings are updated to maintain a correct cycle 5 2 Copy program Select the program you want to copy as described in section 4 1 and 4 2 Directory 3 program no 0 Name TEST 1 lid tem
17. e is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 6 3 Adjust heating and cooling rates Since the T1 Thermocyler is a extremely fast Thermocycler it may be necessary to reduce the heating and cooling ramp for some applications For example this is helpful if cycling protocols from other cyclers shall be used The heating or cooling ramp respectively can be set in the row labeled with A s Main funct dT c dt s A s 2 5 00 3 5 00 4 5 00 be C 5 00 A B header C pgm OK D enter Note The default is 5 00 s which means maximum ramping speed biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 7 Run Program 7 1 Select and start program Main display Prog step temp C time lid A 25 2 C 25 4 C B start stop C programs D To select a program press B start stop start l 3 4 Main direct 5 6 A subdirect subdirect Subdirect subdirect subdirect C quit D enter Choose directory with cursor keys gt ft X or enter main directory with D enter Note The path for the program that was modified most recently is automatically pre selected 3 subdirect l 4 subdirect Main direct 5 Subdirect l 6 subdirect 7 subdirect A C quit D enter Enter subdirectory with D enter Directory 5 program no Name
18. e module 0900000 Install module Follow description in reversed order biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 30 11 Trouble shooting 11 1Slow heating and cooling The T1 Thermooyler is equipped with a strong ventilator for the cooling of the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dirt can be removed easily from the inlet with a conventional vacuum cleaner 11 2 Restart due to unrecognized power failure High voltage fluctuation can lead to an automatic restart of the Thermocycler In this case the cycler restarts at the step where there power failure has occurred To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 11 3Adaptation of protocols from other cyclers Since the T1 Thermocyler is an extremely fast Thermocycler it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 6 3 Alternatively the time settings may be extended Note Protocolls that have been optimized with a TGradient Thermocycler can be run without changes 11 4 Recommendations for the use of 384 well Thermocyclers a Use the recomm
19. ended 384 well plates and sealing materials To prevent sample evaporation by insufficient sealing please use 384 well plates and sealings tested and recommended by Biometra A list of plates and sealing is available at www biometra com gt thermocyclers gt plasticware b Reduce the temperature of the heated lid Due to the fact that the heated lid is very close to the reaction volume with a 384 well plate compared to 0 2 ml vessels the lid temperature can have a significant impact on the effective incubation temperature For reaction volumes less than 10 ul the lid temperature is recommended to be set at 95 C When using a reaction volume of 10 ul or more set lid temperature at 85 C Even at these lowered lid temperatures possible condensation will not occur as problem due to a mixture of physical factors like dew point salt concentration temperature difference air pressure within the tube and reaction volume lt is strongly recommend to reduce the lid temperature according to the reaction volume Otherwise at conventional lid temperature settings sample volumes or amplification yields might be reduced biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 12 Appendix 12 1 Technical specifications T1 Thermocycler 96 3l Thermal range 3 C 100 C Temperature accuracy 0 1 C Thermal homogeneity 0 3 C within 15 sec Maximum heating speed 4 C sec Max cooling speed 3 C sec Samp
20. enter 5 3 Delete program Select program as described in section 4 1 and 4 2 Directory 3 program no 0 Name TEST 1 lid temp ec preheating on edit A copy B delete pgm C quit D enter To delete this program press B delete pgm biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 20 Directory 3 program no 0 Name TEST 1 lid temp ec preheating on delete program 0 enter yes A copy B delete pgm C quit D enter Confirm your selection with D enter Note Once you have deleted a program it cannot be restored biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 21 6 Further programming options Temp C time s opt 1 65 0 Oh 5m Os 2 65 0 Oh 0m30s 3 80 0 Oh 0m30s 4 35 0 Oh Om30s 2 A B header C pgm OK D enter Note To program special parameters you can scroll the display to the right Move the cursor to the right side of the display that is labeled with opt gt The following screen will appear Main funct dT C dt s A s 1 5 00 245 5 00 3 amp 5 00 4 5 00 A B header C pgm OK D enter Note To get back to the screen with the basic parameters move the cursor to the left side of the display labeled with Main funct Alternatively you can move the cursor to the extreme right Temp C time t opt gt 1 65 0 Oh 5m Os 2 65 0 Oh 0m30s 3 80 0 Oh 0m
21. er Enter a time for step one Note There is a convention on how time settings are entered in all BIOMETRA cyclers hours minutes seconds If you enter a number without dot this value will be interpreted as seconds 300 gt 5 minutes To program minutes enter a e after the number of minutes To enter hours enter after the number You can also enter any combination of hours minutes and seconds Example for 1 hour 30 minutes 20 seconds enter 1 30 20 The time values will be displayed in the following format Oh 00 m 00s Accept time setting with D enter or move to the next field with cursor key 4 8 Set cycle number Note In general loops are defined by selecting the target for the back loop and the number of backloops Temp C time 4 opt 2 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 80 0 Oh Om30s 4 35 0 Oh Om30s 2 A B insert delete C pgm OK D enter In the column labeled with you can select the target step for a backloop biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 16 Temp C time opt gt 1 65 0 Oh 5m Os 2 65 0 Oh Om30s 3 80 0 Oh 0m30s 4 35 0 Oh Om30s 2 29 A B insert delete C pgm OK D enter Enter the number of backloops Note total cycler number n backloops 1 e g enter 29 for a total number of 30 cycles 4 9 Cool below ambient temperature Temp C time opt gt 2 65
22. k concept with an advanced block architecture The result is a Thermocycler that provides extremely high temperature ramping rates and an unrivalled temperature uniformity High ramping rates ensure specific reation and the perfect temperature uniformity guarantees identical conditions in any position of the block The T1 Thermocyler is available with different modules that support a broad range of tubes strips and microplates There is also an in situ modul for up to 4 microscopic slides The T1 Thermocyler is adjusted to match the BIOMETRA TGradient cycling characteristics Thus protocols that have been optimized with a TGradient can be operated without the need for modifications This makes the T1 the perfect instrument to increase sample throughput togehter with an existing Tgradient biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 2 Before you start 2 1 Safety precautions Please read this manual carefully before starting operation of the T1 Thermocycler General safety precautions for laboratory work must be observed when working with the T1 thermocycler Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill out Do not heat samples without having the lid locked securely Before opening of the lid release lid pressure see section 3 6 Do not place fingers between lid and housing when opening or closing the lid Do not to
23. le capacity 96 x 0 2ml tubes 96 well microplates strips Heated lid Line Voltage High Precision Smart Lid for optimum lid pressure and excellent temperature uniformity Lid temperature range 30 0 99 0 C 115 Volt or 230 Volt Frequency 50 60 Hz Power consumption 310 watts maximum Fuses see label on the back of the instrument Display LCD graphical display back lit Ports serial RS232 bi directional data transfer parallel Centronics printer Port for in tube sensor memory Total capacity 1500 steps in 10 directories with maximum 100 programs each Dimensions WxDxH 25 x 34 x 16 5 cm Storage conditions Weight 8 8 kg Operating conditions 5 C 35 C 10 90 humidity 12 2 Ordering information T1 Thermocyler Order No T1 Thermocyler 48 for 48 x 0 5ml tubes 050 800 T1 Thermocyler 96 for 96 x 0 2ml tubes strips or 96 well microplates 050 801 T1 Thermocyler 384 for 384 well microplates 050 802 T1 Thermooyler in situ for 4 microscopic slides 050 803 T1 Thermocyler Modules T1 Thermocyler 48 Module for 48 x 0 5ml tubes 050 810 T1 Thermocyler 96 Module for 96 x 0 2ml tubes strips or 96 well 050 811 microplates T1 Thermooyler in situ Module for 4 microscopic slides 050 813 For information about tubes strips microplates and other consumables please contact BIOMETRA or your local BIOME
24. n move the cursor with cursor keys lt gt through the name This is helpful if you have confirmed an wrong letter or if you want to change the name of an existing program Once the name is complete confirm name with C name OK Directory 3 program no 0 Name TEST 1 lid temp ec preheating on edit A B files C pgm OK D enter 4 5 Enter the lid temperature Note Thanks to a new lid design significantly lower lid temperatures than in the past can be used for thermocycling The lower lid temperature leads to a higher temperature uniformity within the lid and thus to a more even temperature distribution in the tubes For instruments featuring the new lid design a maximum temperature of 99 0 C should be used biometra Manual T1 Thermocycler June 2009 a part of Analytik Jena 14 Directory 3 program no 0 Name TEST 1 lid temp 99 C preheating on edit A B files C pgm OK D enter 4 6 Select deselect pre heating of the lid You can choose whether the lid is pre heated before the program starts This is to avoid evaporation during the initial heating phase Note During the preheating of the lid the block is held constant at 25 C Directory 3 program no 0 Name TEST 1 lid temp 99 C preheating on edit A on off B files C pgm OK D enter Note The default setting which is recommended for most applications is preheating on After all pre settings have been made
25. p 99 C preheating on A B files C pgm OK D enter Press B files Directory 3 program no 0 Name TEST 1 lid temp ec preheating on edit A copy B delete pgm C quit D enter To copy this program to another memory press A copy copy to 3 subdirect 4 subdirect Main direct 5 Subdirect l 6 subdirect 7 subdirect A B C quit D enter Select the desired subdirectory with the cursor keys gt ft amp The selected subdirectory will highlight biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 19 copy to 3 subdirect 4 subdirect Main direct 5 Subdirect 6 subdirect 7 subdirect A C quit D enter Confirm your selection with D enter copy from directory 3 program 0 to directory 6 program A list B C quit D enter Choose an empty memory from the list with A list copy from directory 3 program 0 to directory 6 program 6 enter OK target program contains 0 steps A list B C quit D enter Note Before you confirm with D enter the target memory is checked If there is no program in the target memory the message target program contains 0 steps is displayed If the memory already contains a program the number of steps is displayed Note If you copy a program to a memory that already contains a program the former program will be deleted Save a copy of the program in the new directory with D
26. program without name For the programming of new protocols choose an empty memory Existing programs that are not needed any more can be deleted to provide new memory empty empty empty empty empty list B forward C quit D enter PaUNHPO Scroll through the list with cursor keys 9 and confirm memory with D enter 4 4 Enter a program name Each program is specified by a program number and a subdirectory number To make retrieval of a program easier you can enter a name for each program existing of letters numbers and symbols Directory 3 program no 0 Name ABCDEFGHIJKLMNOPORST UVWXYZ C Q amp A ABC B files C name OK D enter To enter a name for your program press A ABC biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 13 Directory 3 program no 0 Name ABCDEFGHIJKLMNOPORST UVWXYZ C amp A name B blank C quit D enter Choose a letter from the alphabet with cursor keys gt t 4 Confirm each letter with D enter Directory 3 program no 0 Name T ABCDEFGHIJKLMNOPORST UVWXYZ C amp A name B blank C name OK D enter The selected letter will appear in the display and the cursor will move one step to the right Select the next letter and confirm with D enter Note What to do if a wrong letter has been accepted To alter the position of the cursor within the name press A name Now you ca
27. r laboratory COMPANY INSTITUTE ADDRESS TEL NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Has the equipment been cleaned and decontaminated YES NO delete Method of cleaning Decontamination NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 35 16 EU Konformit tserkl rung EU Declaration of Conformity G ttingen den 20 08 2004 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within certain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erkl ren wir daB folgende Thermocycler und Thermocycler Module Herewith we declare that the following thermocyclers and thermocycler moduls Typen T1 Thermocycler 48 T1 Thermocycler 96 T1 Thermocycler Kom
28. rage place the subdirectories can be named see section 4 3 Start with the main screen Prog step temp C 25 29C time lid 25 4 C A B start stop C programs D Press C programs to enter the edit mode 3 subdirect l 4 subdirect Main direct 5 Subdirect l 6 subdirect 7 subdirect A C quit D enter You are now in the main directory The structure of the subdirectories is displayed 4 2 Select a program store Programs can be stored in the main directory or in subdirectories To create a program in the main directory press D enter To access a subdirectory move right with the cursor key gt and select a subdirectory with the cursor keys 4 The selected directory will be highlighted Main direct A N AUT P W subdirect subdirect Subdirect subdirect subdirect C quit D enter Manual T1 Thermocycler June 2009 biometra a part of Analytik Jena 12 4 3 Enter name for subdirectory The process for naming a subdirectory and a program is the same For information about naming programs see section 4 4 Press D enter to enter the highlighted subdirectory directory 5 program no Name edit A list B C quit D enter Press A list to view a list of all existing files and empty memory in this directory Note If neither program name nor the word empty is displayed this place may be occupied by a
29. t rio deu SU NC NL SS DAS PNG Diese 11 4 2 Select program Store cere nee 11 4 3 Enter name Tor subdirectory a A ee 12 4 4 Enter a Program NaMe zentrieren ee 12 4 5 Enter thelid temperature iiion RR 13 4 6 Select deselect pre heating of the lid 444 u444444400nnnnnnnnnnnnnnnannnn nn nannnnnnnnn 14 4 7 Enter temperature and time settings ussssnsnnnnnnnnnannnnnnnnnnnnnnnannnnnennn nenn 14 4 8 Set cycle number eet RE Reo DII pe bed ibm ue nte egi aba ises 15 4 9 Cool below ambient temperature esses eene nennen 16 4 10 Save Program essen ettet te bist ln Ban een 16 Viewiedit Program dalaa u a ee 17 5 1 Delete insert program steps ummenrsnnsnnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnn nenn nn 17 5 2 CODY DEOSI aio caos rts et ipa eier 18 5 3 DSI SIG program eM RUN NEUE 19 Further programming options n tete eek 21 6 1 Program tume anerereniis oe eee Dom eoe eniin E on needs cadet ae ag iin 21 6 2 xo f iabre epNeoj oO 22 6 3 Adjust heating and co ling rates ennen es 22 PRUNE rero In etc 23 7 1 Select and start programs ee 23 7 2 Display during oper tion u el 24 7 3 View remaining run tii seco e ooo E ERR ie 25 7 4 Pause stop Program sso it i dedero RO erbe Renten lcetumn da eid 25 Special functions NER 27 8 1 Print prolo60lsu a ti ee 27 8 2 SWIICHBESB O NOT ns a ee I E 27 8 3 Select language esse 28 8 4 RS232 CONIO T
30. tik Jena 28 8 3 Select language The display of the T1 Thermocyler can be switched from English to German Select language with cursor keys ft As you toggle between the two settings the language will change language A B German English C quit D enter To confirm your selection press D enter 8 4 RS232 Control The T1 Thermocylcer can be programmed and controlled by a Personal Computer The Tcontrol iT software is available with the order number 050 820 To acivate remote control the T1 Thermocylcer has to be set to RS232 control mode For detailled information on controlling T1 Thermocylcer with PC please refer to the Tcontrol iT manual biometra a part of Analytik Jena Manual T1 Thermocycler June 2009 29 9 Maintenance The T1 Thermocyler was built to operate for a long time without the need for periodical maintenance The housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning 10 Exchange of modules For exchange of modules you need two hexagonal keys number 3 and number 5 These keys are included in the shipment of the module Remove module Remove power plug Place instrument upside down on a soft surface Remove the 2 screws that close the shutter hexagonal key number 3 Remove the 4 screws that hold the block hexagonal key number 4 unplug connectors from the block control and power supply remov
31. uch the heated lid When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Use only suited plastic ware in the T1 thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids The T1 Thermocyler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible The T1 Thermocycler must not be used with explosive flammable or volatile liquids Appropriate safety regulations must be observed when working with infectious or pathogenic material The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes gt BD If you still decide to use oil do not
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