Little Dipper Processor User Manual
Contents
1. D Calibrating the Temperature Controller The EZ Zone controllers are calibrated at the factory to provide accurate bath temperatures from ambient 35 to 95 C Re calibration is recommended only if the display varies by more than one degree 1 C to the temperature shown on an externally connected NIST certified digital thermometer Cat 1051 52 0 To adjust a controller to achieve accurate temperatures 1 Turn ON the instrument and set the controller to 65 C Allow 30 minutes for temperature to stabilize 2 Using the cable provided with the digital thermometer plug one end into the blue receptacle found above the controller 3 Turn on the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the bath will be displayed 4 Calculate the difference between the temperature shown on the controller and on the digital thermometer For example if the controller displays 65 0 C and the thermometer displays 66 5 C the difference offset is 1 5 C 5 On the controller press the up and down arrows simultaneously for 3 seconds The left display shows A1 and the right display shows open 6 Press the Advance Button green circle 3 times until the right display shows i CA The left display shows the offset value between the controller and thermometer when the unit was last calibrated 7 Use the up or down arrow to adjust the offset value to the temperature difference2. Select the remaining Step 1 parameters and enter the desired values within the ranges indicated below if so equipped e Agitation Speed 0 to 600 e Agitation Time 0 32 000 seconds e Stroke Length toggle selection of std or long e Drip Time 1 32 000 seconds time rack is held above bath before next step e Pause Time Timed Pause enter 1 32 000 seconds for rack to remain submerged at the end of a step After time elapses the protocol resumes automatically User Control Pause enter 9999 for rack to remain submerged at the end of a step until the operator presses the touchscreen to resume the protocol This is useful for protocols that require more than 5 baths When all values for Step 1 are entered press Save and Exit to return to the Step Input screen 17 408 733 7337 techserv scigene com _SCIGeNE Limie Dippers Processor USER MANUAL 7 Succeeding steps max 9 are programmed and saved one by one From the Step Input screen select the Step enter the desired values then press Save and Exit Repeat for each remaining step tet 2 Parameters ATE STROKE PAUSE M LENGTH TIME sath lAGITATE lhc NUMBER SPEED SLO A come Seuss amp EXIT CANCEL Step Parameter screen with Drip Time Step Parameter screen with Stroke Length 8 To use centrifugal drying select C for the final step in the protocol and enter the number of seconds for the spin No further step
3. g Centrifuge Bucket Assemblies Always use the correctly sized centrifuge buckets spacers and pads matching your sample rack 24 position large or 12 position small Toujours utiliser les taille correctement centrifugeuses seaux entretoises et patins correspondant votre rack de l chantillon 24 positions grand ou 12 positions petite To install a centrifuge bucket assembly Version 2 1 May 2015 Unlock and open the centrifuge door Gently squeeze the side tabs of the red bucket and slide it onto the pegs of the rotator arms marked BALANCE RACK Gently squeeze the side tabs of the green bucket and slide it onto the pegs of the rotator arm marked SAMPLE RACK Place an absorbent pad in each bucket then a black frame spacer on top Place a slide rack into the red balance bucket with an equal number of slides as those to be processed Close and lock the centrifuge 24 Slide Rack Assembly 12 Slide Rack Assembly JL a ie aR 24 Slide Rack Pi Ss i NARRID o tA sas SS 12 Slide Rack Te Spacer large in 5 ma pacer sma A Absorbent pad small I S il Ae Centrifuge Bucket large SE YY a Absorbent pad large Centrifuge Bucket small 9 408 733 7337 techserv scigene com lil USING YOUR LITTLE DIPPER PROCESSOR A Components Controls and Accessories 7 Gripper arm 8 Gripper 11 Slide rack 9 Fan in back not sho
4. Wipe the spill using an absorbent material Always pull the liquid to the front of the instrument away from the control panel and around the bath positions Wipe the deck dry with a dust free towel and 70 ethanol or similar Dispose of the material in accordance with good laboratory practices 2 Disposing of Reagents Chemicals used on this instrument may be hazardous Adhere to good laboratory practices when disposing of any hazardous chemicals Version 2 1 May 2015 6 408 733 7337 techserv scigene com _sciGene inie Dipper Processor USER MANUAL G Compliance Electromagnetic Compatibility CE 1 All instruments shipped to the European Union EU formerly known as the European Community have the CE label on the back of the instrument signifying that these instruments comply with the Electromagnetic Compatibility and Low Voltage Directives 2 Restriction of Hazardous Substances RoHS The RoHS directive was adopted in February 2003 by the European Union It restricts with exceptions the use of six hazardous materials including lead RoHS and mercury in the manufacture of various types of electronic equipment All SciGene instruments carrying the CE mark are also ROHS compliant 3 Electrical and Electronic Equipment Waste WEEE WEEE is a European Community directive that became European Law in February 2003 The crossed out wheelie bin symbol on a product or its packaging indicates that it
5. For Continued Protection Against Fire Replace Only with Same Type Rating of Fuse Always disconnect the power cord before attempting to replace the fuse Ce symbole sur l arri re de l instrument indique la pr sence de la bo te de fusibles Avertissement Pour le maintien de la Protection contre l incendie remplacer uniquement avec la m me cote de Type de fusible Toujours d brancher le cordon d alimentation avant d essayer de remplacer le fusible Always insert a bath liner and fill with buffer before turning on power to each bath Toujours ins rer une doublure de bain et remplir avec un tampon avant de l allumer chaque bain The instrument weighs approximately 59 Ibs 26 5 kg Use caution when lifting the unit to avoid personal injury L instrument p se environ 26 5 kg Soyez prudent lorsque vous soulevez l unit pour viter toute blessure Do not under any circumstances lift the unit by the cylindrical tower or its extended gripper arm This will damage the instrument Ne pas en aucun cas soulever l unit par la tour cylindrique ou son bras de pr hension prolong e Cela pourrait endommager l appareil 5 408 733 7337 techserv scigene com un A C D gt D gt gt gt Little Dipper Processor USER MANUAL Never run a protocol while a bath cover is installed Ne jamais faire fonctionner un protocole tandis qu un couvercle de la cuve est install e To achieve temperatures above 70 C a bath
6. gt gt gt EP PE PP Version 2 1 May 2015 This symbol indicates the potential for a pinch point Do not place fingers in the slot in the Z axis tower Ce symbole indique la possibilit d un point de pincement Ne placez pas les doigts dans la fente dans la tour de l axe Z This symbol indicates the potential presence of a hot surface Turn off the temperature controller and leave sufficient time for cooling or use heat protection prior to handling a bath or cleaning in this area Ce symbole indique la pr sence potentielle d une surface chaude Eteignez le contr leur de temp rature et de laisser suffisamment de temps pour le refroidissement ou utilisez une protection de chaleur avant de manipuler un bain ou de nettoyage dans ce domaine Do not touch the bottom metal surface of the bath during operation Ne touchez pas la surface du m tal fond de la baignoire pendant le fonctionnement Biological hazards can be associated with the materials used on this instrument Potentially infectious waste and other hazardous waste must be treated in accordance with good laboratory practice guidelines Les dangers biologiques peuvent tre associ s des mat riaux utilis s dans cet instrument Potentiellement d chets infectieux et d autres d chets dangereux doivent tre trait s conform ment aux lignes directrices de bonnes pratiques de laboratoire This symbol on the rear of the instrument indicates the presence of the fuse box Warning
7. LITTLE DIPPER PROCESSOR cscscscscscscscsccscscscscscscsscscscscscscscscsceacs 10 A Components Controls ANd Accessories ses 10 B Touch Screen and Power Connections cccccssecccssscccesececenececenececescesaueceseeceeeueessuencessuecenenees 11 C Using the Temperature Controller sisi 12 D Calibrating the Temperature Controller 12 PMs SYTEM OPERATIONS ne ness s E ee nano ee est ane coca essieu 13 A LINE Me TOCS Or OEN a era da a a ae ne ae ie 13 Be Lise tie Pause BEM Se de a see caen A a ei 13 GC Using th Emergency Stop EMO herenera a E ere nana mence rome clercs 13 D Usine Pre Heating Bath Covel irespeto EE ae ot a ea Do da nie 13 E Using a Slotted Bath Cover for Prolonged High Temperature Immersion ccccseecceeeeeeeeeees 14 Pe OCIS SORA a Da ae ae a 15 G RUNNING a Programmed PrOotOCOl usa sonetasnesencetianeseinete etes see teens ave ansues 16 V USING CUSTOM PROTOCOLS visa ciccccsecccssccnsevscseccssendenseseannsciaescieseesssaisnieneesansenccetoarnencene 17 A Creating or Editing a Protocol csscccssssscssssccssseccnsvescusescessecesseseusseneuseseneuseceuseseussessesesess 17 B ReMAMING A PIOUOCO siacscrtcccsdcnnassaanediencesitesinwondessapsavanapialaioatensaasiatiensaanabastodeedeawosveuamaadesanbarsadons 18 C Deleting PE a e a aa a ia oiia 18 VI MAINTAINING THE LITTLE DIPPER PROCESSOR cscscccsccscsccscscsceccscscsscscscsscscecs 19 A PONa O e
8. T E Oo 19 B Cleaning Belore Every DS a a a a S desanecieuesocteiancdeauats 19 Cy Cleaning Anter Every US en E S 19 D Periodic Cleaning and Calibration ssssessensssenssressserssressrrrssrereseesserrssrresrerserersrrreseeeseeessereseeee 19 VH TROUBLES FO OTN Gav sn a nEn EEEE E EEEE 20 VII SPECIFICATIONS nsc nos OEN EEEE EA EEEE 20 IX ORDERING INFORMATION ee uses aeie EEA 20 X DECLARATION OF CONFORMITY sisccescetsntanvessisinnsesescuncatcdoncesteteanviavonavesteecdesinetestaseiacueeds 21 Version 2 1 May 2015 2 408 733 7337 techserv scigene com _sciGene utcie Dipper Processor USER MANUAL I SAFETY NOTICES A Intended Use The Little Dipper Processor automates slide washing and drying for applications including FISH fluorescence in situ hybridization G Banding and microarray analyses Le processeur automatise le lavage et le s chage de diapositives pour les applications y compris FISH hybridation fluorescente in situ G bandes et des analyses de micropuce Instrument Safety Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument Avant d utiliser l instrument lire les informations dans cette section concernant les dangers et les risques potentiels Assurez vous que toute
9. pratiques de laboratoire This symbol indicates the presence of a fire or explosion hazard Consult this Operator s Manual for further information Ce symbole indique la pr sence d un risque d incendie ou d explosion Consultez le manuel de l utilisateur pour plus d informations gt amp PP D Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death D faut de respecter les avertissements suivants qui sont appos es sur le produit peut conduire possibles l sions corporelles ou la mort This symbol indicates the presence of hazardous voltage Always disconnect the AN power cord before attempting any repairs Ce symbole indique la tension dangereux de pr sence Toujours d brancher le cordon d alimentation avant de tenter toute r paration Warning Do not use in the vicinity of flammable or combustible materials Fire or explosion may result Avertissement Ne pas utiliser proximit de mati res inflammables ou combustibles Incendie ou une explosion peuvent en r sulter Version 2 1 May 2015 4 408 733 7337 techserv scigene com SciGene E Cautions Little Dipper Processor USER MANUAL Failure to comply with the following cautionary statement affixed to the product may lead to possible personal injury Omission de se conformer la mise en garde suivante appos e sur le produit peut entrainer des blessures possibles
10. 00e www scigene com Little Dipper Processor USER MANUAL FISH Cat 1080 70 1 115V and 1080 70 2 230V Agilent Cat 1080 40 1 115V and 1080 40 2 230V Illumina Cat 1080 30 1 115V and 1080 30 2 230V Affymetrix Cat 1080 65 1 115V and 1080 65 2 230V Self spotted Cat 1080 50 1 115V and 1080 50 2 230V FOR RESEARCH USE ONLY 1287 Reamwood Avenue Sunnyvale CA 94089 USA e Tel 408 733 7337 Fax 408 733 7336 techserv scigene com _sciGene urcie Dipper Processor USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the electrical instrument described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable baths or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to provide replacement parts at no charge or at its sole discretion replace the product The foregoing warranty is void in the event the unit was abused or modified or used ina manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a part
11. calculated in Step 4 For example if the controller shows a temperature 1 5 C lower than the thermometer adjust the offset by adding 1 5 to the value shown on the display 8 Press the Infinity Key ee twice to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display The controller is now calibrated to provide accurate temperatures from 35 to 95 C Version 2 1 May 2015 12 408 733 7337 techserv scigene com _SCIGeNE Lime dippers Processor USER MANUAL IV SYSTEM OPERATION A Using the Touch Screen Use the handheld touchscreen to navigate between screens select and run protocols create new protocols and control instrument movement If the touchscreen fails to respond it may require calibration See page 10 for details B Using the Pause Button Instrument movement can be stopped and resumed during a protocol using the red PAUSE RESUME button at top right on the touchscreen When pressed the instrument will stop and the Touchscreen button light will flash To resume the protocol press pause again C Using the Emergency Stop EMO To immediately stop movement of the gripper arm and stir bars press the red Emergency Stop EMO button on the right side of the instrument Bath heaters will continue to receive power but automated movement will cease and touch screen will turn off Restore power to the gripper arm and stir bars by rotating the EMO but
12. cover must be used to obtain and maintain temperatures When the robot is in use baths running at high temperatures may need to be topped off due to evaporation Pour atteindre des temp ratures sup rieures 70 C une couverture de bain est utilis Lorsque le robot est en fonctionnement des bains haute temp rature peuvent avoir besoin de tampon suppl mentaire Avoid spills on the cabinet vitez les d versements sur l armoire F Spills and Reagent Disposal Observe all national regional and local regulations for waste disposal and management Respecter toutes les r glementations nationales r gionales et locales pour l limination des d chets et management Biological and other hazards can be associated with the materials used on this instrument Potentially infectious and other hazardous waste materials must be treated in accordance with good laboratory practices Dangers biologiques et mat riaux potentiellement infectieux et d autres d chets dangereux peuvent tre utilis es sur cet instrument et doivent tre trait s conform ment aux bonnes pratiques de laboratoire This symbol indicates the potential presence of a hot surface Use care when cleaning a spill in this area to avoid being burned Ce symbole indique la pr sence potentielle d une surface chaude Faites attention lors du nettoyage d un d versement pour viter d tre br l 1 Cleaning Spills a b C d Turn off the instrument
13. e free of buffer residue and dust before processing slides If they were not cleaned and stored properly after the last use wash and dry them as follows oY SS SY Fill baths containing the rack s and stir bars with tap water Wipe racks stir bars and baths with a lint free towel Rinse in running tap water Rinse with approximately 100 200 ml of DI water Rinse with 100 ml of ethanol and dry with a lint free towel Clean temperature sensors with DI water and a lint free towel C Cleaning After Every Use 1 2 3 Wash baths stir bars and racks with running tap water then rinse in DI water and ethanol and dry with a lint free towel Store in clean plastic bags to protect from dust Clean temperature sensors with DI water and lint free towel Wipe exterior of instrument cabinet with a damp paper towel D Periodic Cleaning and Calibration 1 Calibrate bath temperatures using a NIST certified thermometer See section VI A Temperature Controller Calibration Remove and wash the centrifuge buckets and spacers using warm water and lint free towels Replace the absorbent pad in each centrifuge bucket Clean all exterior surfaces of the cabinet gripper paddles and gripper arm housing with damp lint free towels Remove the centrifuge buckets Clean the inside surfaces of the centrifuge and rotor with damp lint free towels Inspect the centrifuge buckets for signs of wear or deformation where they hang from
14. g a protocol 1 2 aa Place a slotted bath cover over a bath for pre heating Program a protocol to include two User Control Pauses one at the step where the cover is placed and one prior to that step The step with the cover should be programmed with 0 agitation speed Start protocol and wait for slide rack to pause in the bath prior to the covered bath Remove the slotted bath cover Press anywhere on the touch screen to resume protocol and move rack to the high temperature bath The rack will remain submerged for the programmed agitation time at O agitation speed Slide a slotted bath cover around the rack handle and temperature sensor At the end of step the touch screen will beep to alert the operator to remove the slotted bath cover Slide the slotted bath cover away from the rack handles and temperature sensor to remove Press anywhere on the touch screen to resume protocol EF Si Was haa se Eikan Insert a slotted bath cover to maintain high temperatures and reduce evaporation Take precaution when removing a bath cover since buffers and slide rack will be hot Prendre des pr cautions lors de la suppression d un couvercle de bain depuis des tampons et la grille coulissante seront chauds Version 2 1 May 2015 14 408 733 7337 techserv scigene com F Loading a Slide Rack The pre loaded Quik tst protocol provides the operator an opportunity to practice loading the slide rack onto the gr
15. icular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damages or for any damages resulting from loss arising out of or in connection with the sale use or performance of the product Copyright Copyright 2005 2015 SciGene Corporation All rights reserved SciGene and Little Dipper are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Version 2 1 May 2015 1 408 733 7337 techserv scigene com _sciGene urcie Dipper Processor USER MANUAL Table of Contents k SAFET INO 2 1 CRE Eee ee 3 SU a ee 3 SERA Se a da a De de da en ea een 3 C Symbols and CONVENTIONS ccccesccccsssccceseccceneeccencccaesececeeceeeneceeencceaueecesseceseeeeseueessugecesseeeeees 3 D WANE era A E E E E O 4 E CONS pa E E RE E A 5 F Spills and Reagent Disposal sensssensssenssensseessrerssrereserssresssrresreeserersrrrssressreesrersseerosrerseeeseereseeesse 6 G CONDUCE ar E E ee eee en rere 7 i UNPACKING AND SETUP nd nes e naaa 8 As UNDI RIRE Che INEN E neee AE 8 Be Pone POI E ee eee ae 8 C Environmental R QUIFeM NES 222 es EN EEEE Ari 8 D IAstalation and PO CAMO sasctsa ced cancccanctaesicatasientgesecensusecaaansnesinbetaaimngeseasbessacionnendeieavenarsongeaastedmiccuaes 8 E WS CNN BAS a aan ne ete de ne ne ed 9 F Installing Centrifuge Bucket Assemblies ss 9 Ill USING YOUR
16. ipper An empty slide rack is moved automatically from bath to bath with up down agitation followed by brief centrifugation No liquid is required Power switches for baths should remain in the OFF position 1 Ensure that baths and centrifuge buckets are properly installed and correspond to size of the slide rack to be used Place an empty slide rack in the red balance bucket in the centrifuge Verify all temperature sensors are rotated down and power to all baths are turned off Turn on main power located on the right side of the instrument Using the touchscreen press Run Protocol from the home screen and select Quik tst Hold an empty slide rack in your left hand centered in Bath 1 and aligned with the black lines on the instrument as shown below 7 Press Start Now on the touchscreen The arm will lower into Bath 1 with the gripper open When the gripper arm reaches the end of its downward travel align the holes in the handle with the two bumps in the gripper paddle as it slowly closes oa SY x Hold slide rack in Bath 1 to load Release rack as gripper closes Use thumbpad to load or remove rack manually If the slide rack does NOT load properly press the pause button and use the thumbpads on the gripper to manually open and load the rack Press pause again to continue Si la grille coulissante ne se chargent pas correctement appuyez sur le bouton de pause et d utiliser les thumbpads sur la pince ouvrir manuel
17. lement et charger le rack Appuyez sur Pause pour continuer 8 The instrument will agitate the rack in each bath and then transfer it to the centrifuge When the protocol is complete unlock and open the centrifuge lid to remove the rack Version 2 1 May 2015 15 408 733 7337 techserv scigene com _sciGene utcie pipper Processor USER MANUAL G Running a Programmed Protocol The instrument comes configured with validated protocols installed Refer to the Little Dipper Processor Method corresponding to your protocol for details on how to set up the instrument Method sheets can be found online at www scigene com gt gt A Bath covers must be removed before starting any protocol unless the protocol includes a User Controlled Pause in the step prior to the bath with the protocol Couvercles de bain doivent tre enlev s avant de commencer tout protocole et ne peuvent pas tre utilis s pendant le fonctionnement To achieve temperatures above 70 C use a bath cover while the machine is NOT in motion Pour atteindre des temp ratures sup rieures 70 C utiliser un couvercle de bain pendant que la machine est en mouvement ou pas un couvercle fendu pendant le fonctionnement Baths at high temperatures may need buffer s added to offset evaporation Bains des temp ratures lev es peuvent avoir besoin de tampon s ajout pour compenser l vaporation The following are general guidelines on how to set up and run a
18. must NOT be disposed of with standard waste at the end of its lifetime Instead it is your responsibility to return it to a designated collection point for electronic equipment recycling Contact your local SciGene distributor for information on drop off locations Laboratory Equipment Standards This symbol indicates the instrument meets Laboratory Equipment standards UL 61010 1 2004 R7 05 and CAN CSA C22 2 61010 1 2004 as certified by T V Rheinland of North America Inc 5 Electromagnetic Compatibility EMC The Little Dipper Processor is a Class A digital device under FCC Title 47 Part 15B and designated as Class A electrical equipment for measurement control and laboratory use EN61326 Note regarding Canadian EMC compliance Le present appareil numerique n emet pas de bruits radioelectrique depassant les limites applicables aux appareils numeriques de class A prescrites dans le reglement sur le brouillage radioelectrique edicte par le Ministere des Communications du Canada Version 2 1 May 2015 7 408 733 7337 techserv scigene com II UNPACKING AND SET UP A Unpacking the Instrument The Little Dipper Processor weighs about 59 Ibs 26 5 kg and is generally shipped in two cartons A large carton contains the processing unit along with an interior box for the touchscreen power cord and user manual The second smaller carton contains baths racks and other accessories depending on configuration Caution is needed when lif
19. nstant D Les power Position the instrument with 6 inches of R By ENS 17 clearance at the back to allow adequate air flow of the ii cooling fan and ensure unimpeded access to the main power and emergency shut off EMO switches on the right side of the instrument R A E Nae UT i y g W wid 94 sayoul 0 30 inches 76 cm Version 2 1 May 2015 8 408 733 7337 techserv scigene com SciGen E Installin 1 2 3 A F Installin A e Little Dipper Processor USER MANUAL g Baths Clean baths slide racks and stir bars by rinsing in running tap water followed by 100 ml of DI water and then 100 ml of ethanol Dry with a lint free towel Clean temperature sensors with DI water and a lint free towel Insert baths in the black basins Rotate temperature sensors up prior to inserting any standard or low volume heatable LVH baths and then ensure all temperature sensors are lowered down prior to starting a protocol Place a stir bar in each bath Never pour any liquid into the black basins A removable bath must be inserted prior to adding buffer Ne jamais verser de liquide dans les bassins noirs Un bain amovible doit tre ins r e avant d ajouter tampon Never heat a bath without buffer Baths may need more buffer added due to evaporation Ne chauffez jamais un bain sans tampon Bains peuvent avoir besoin de davantage de tampon ajout e due l vaporation
20. personne impliqu e avec le fonctionnement de l instrument est charg dans les deux pratiques g n rales de s curit pour les laboratoires et les pratiques de s curit sp cifiques pour l instrument Symbols and Conventions The following list is an illustrated glossary of the electrical symbols that are used on the Little Dipper Processor Whenever such symbols appear on instruments please observe appropriate safety measures Le tableau suivant est un glossaire illustr des symboles lectriques qui sont utilis es sur le systeme Chaque fois que ces symboles apparaissent sur les instruments veuillez observer les mesures de s curit appropri es 1 Electrical Symbols This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument Ce symbole indique qu il s agit d un terminal de terrain prot g qui doit tre connect la terre avant que toutes les autres connexions lectriques sont apport es l instrument CAUTION This symbol alerts you to an electrical hazard Please consult this AN User Manual for further information and proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der avec prudence This symbol indicates the OFF position of the main POWER switch Ce symbole indique la position OFF de l interrupteur principal This s
21. pre installed protocol pr h A e 10 LE 12 13 14 Version 2 1 May 2015 Ensure baths racks and stir bars are clean and free of buffer residue and dust Insert baths and stir bars as specified for the protocol Rotate all temperature sensors down Add recommended buffer s to the fill line on the side of the baths Turn on main power to the instrument Turn on power to bath s to be heated and set temperature s Use a standard bath cover for temperatures above 70 C to reduce heating time and evaporation Allow time for baths to reach set temperature s Open the lid of the centrifuge and place a slide rack with the same number of slides to be processed into the red balance bucket Add the slides to be processed into the sample rack Remove any bath covers Submerge the slide rack in Bath 1 using your left hand between the two black lines On the touchscreen home page press Run Protocol then select the desired protocol and press Start Now When the gripper arm stops in the down position in bath 1 align the holes of the slide rack handle onto the bumps gripper paddles and wait for the gripper to close The instrument will now perform the steps as programmed At completion remove the rack from the centrifuge Some protocols end with the rack remaining in the gripper Open the gripper to release the slide rack by pushing the thumb tab on the rear gripper paddle Clean the instrument per section VI C Cleaning Afte
22. r Every Use 16 408 733 7337 techserv scigene com _SCIGeNE Lime Dippere Processor USER MANUAL V USING CUSTOM PROTOCOLS A Creating or Editing a Protocol Custom protocols can be created using the touchscreen The touchscreen can store up to 14 protocols with 1 to 9 steps each All protocols start from Bath 1 1 Version 2 1 May 2015 From the touchscreen Home page press New Edit Protocol On the Create Edit Protocol screen use the up down buttons to move the cursor to NEW Press Select to go to the Name Protocol screen If editing a protocol select it to go directly to the Step Input Screen to make the desired changes Within the Name Protocol screen use the shift key to move the up arrow within the alphanumeric 10 key pad until the desired letter or number is indicated Select up to eight characters When finished press Enter to go to the Step Input screen and then press Step 1 Depending on your instrument configuration you will have options for either drip time or stroke length but not both NEW_TST STEP INPUT BATH _AGITA TE RI il 4 cem secs er NEW TST STEP INPUT BP AGITATE PAUS SPeeq secs SsLrOorke sec v 1G i v NaN Coat SAVE r cat ET Br Step Input screen with Drip Time option Step Input screen with Stroke Length option When the Parameters screen displays press the Bath button enter 1 and then press Enter Step 1 must always originate from Bath 1
23. s may be programmed after a centrifuge step ster 2 Parameters j BATH i NRBER CENTRIFUGE a CANCEL Step Parameter screen for Centrifuge spin time 9 When all protocol steps are entered press Save amp Exit to return to the Select Protocol screen Up to 3 steps are displayed at one time on the touch screen B Renaming a Protocol 1 Torename a protocol press Edit Protocol from the touchscreen Home page 2 Use the up down buttons to move to select the protocol to edit 3 Select Change Name 4 Use the shift key to move the up arrow within the alphanumeric 10 key pad until the desired letter is indicated to input the new name Select up to eight characters 5 When finished press Enter un C Deleting a Protocol 1 To delete a protocol press Control Panel from the home screen then Advanced Settings 2 Press the Delete Protocol button to open the screen 3 Choose a protocol to delete using the up and down arrows and then press Select A confirmation screen appears saying Delete Protocol Choose YES to confirm 4 Press the BACK button on each of the previous screens to return to the home screen Version 2 1 May 2015 18 408 733 7337 techserv scigene com VI MAINTAINING THE LITTLE DIPPER PROCESSOR A Powering Off Turn the power switch to the OFF position and unplug the power cord before performing any service procedure B Cleaning Before Every Use Baths racks and stir bars must b
24. surement control and laboratory use to include EN 61000 4 2 1995 1998 A2 2001 EN 61000 4 3 2006 EN 61000 4 4 2004 EN 61000 4 6 2007 LVD Directive 2006 95 EC EN 61010 1 2010 Safety requirements for measurement control and laboratory use 3rd Edition Part 1 General requirements IEC 60204 1 2006 Electrical equipment of machines RoHS Directive 2011 65 EU Terry Gill Sunnyvale California USA Name of Authorized Representative Place of Issue Director of Product Manufacturing January 02 2014 Title of Authorized Representative Date of Issue 1 Ptll Signature of Authorized Representative Version 2 1 May 2015 21 408 733 7337 techserv scigene com
25. the rotor Replace if worn or bent Version 2 1 May 2015 19 408 733 7337 techserv scigene com _sciGene atthe Dipper Processor USER MANUAL VII TROUBLESHOOTING Instrument does not turn on Blown fuse s Replace fuse s near main power switch Touch screen does not illuminate Not connected or Check connections and rotate EMO when main power is on EMO is engaged switch to release the emergency stop Touch screen does not respond Touch screen is Contact SciGene or your local distributor when buttons are pressed faulty for assistance VIII SPECIFICATIONS Voltage 115V AC 50 60 Hz 20A or 230V AC 50 60 Hz 10A Size HxWxD 20 x 28 x 22 inches 51x 71 x 56 cm Weight 59 Ibs 26 5 kg net Performance and Controls IX ORDERING INFORMATION Tene serpin Sd 2010 00 1 FISH Wash Buffer 1 0 4xSSC 0 3 IGEPAL pH 7 4L Store at RT 2010 00 2 FISH Wash Buffer 2 2xSSC 0 1 IGEPAL pH 7 4L Store at RT EA Version 2 1 May 2015 20 408 733 7337 techserv scigene com _sciGene urcie Dipper Processor USER MANUAL X DECLARATION OF CONFORMITY Little Dipper Processor SciGene 4 1287 Reamwood Avenue Sunnyvale CA 94089 USA Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 2004 108 EC Standards EN 61326 1 2006 Electrical equipment for mea
26. ting the unit to protect yourself and others from personal injury Two people are required to lift the unit one on each side keeping it balanced and sharing the load 1 Open the large carton and remove the protective foam 2 With the assistance of another person place fingers under the bottom of the instrument and carefully lift out and place on the bench 3 Cut the white tie wrap securing the gripper arm NEVER lift the unit by the robot arm Always grip from the bottom when lifting If the instrument is damaged upon arrival please retain all shipping materials and contact SciGene or your local distributor for assistance B Parts Provided The following items may be included with the instrument or in separate carton depending on configuration e Touchscreen with cable e Baths e Power cord e Slide racks e Digital thermometer e Pads spacers or covers e Centrifuge buckets e User Manual C Environmental Requirements Ensure that the area where the instrument is installed meets the following conditions for reasons of safety and performance 15 to 32 C 58 to 90 F Relative humidity 20 to 80 RH non condensing Air flow clearance 6 inches 15 cm minimum at back of unit D Installation and Location Place the instrument on a lab bench or stable level surface of at least 30 x 22 inches 76 x 56 cm with a vertical clearance of 30 inches 76 cm within a few feet of a power source that delivers 20 Amps of co
27. to the Fuse Holder touchscreen and to the receptacle ra labeled TOUCHSCREEN on the right side Power Cord f Connection of the instrument using the circular connectors y Touchscreen 2 Connect the power cord to the RE lt Cable Connection receptacle labeled POWER on the right side of the instrument and to a power source that delivers 20 Amps or more for 115V units or 10 Amps or more for 230V units Avoid connecting to a circuit with any other equipment Touchscreen that draws significant power Pause Button 3 Turn onthe main power switch on the right side of the instrument The touchscreen will illuminate and motors will activate Version 2 1 May 2015 11 408 733 7337 techserv scigene com _sciGene inie Dipper Processor USER MANUAL C Using the Temperature Controller The Little Dipper Processor is equipped with a Watlow RER PARUS ssh EZ Zone temperature controller calibrated at the factory to provide accurate temperatures from 35 to 95 C The controller has two LED displays and four push buttons The left display shows ACTUAL temperature in red The right display shows the SET or programmed temperature Advance Button Up Down Arrows in green To enter the SET temperature simply push the Infinity Return Key up and down arrows until the desired temperature is shown in the green display on the right The instrument will adjust the heat until the SET temperature is attained
28. ton a quarter turn clockwise Emergency Switch A Press to stop a protocol Twist clockwise to resume The protocol will NOT resume upon releasing the EMO switch and must be restarted Rel chez le commutateur de EMO pour d marrer le protocole D Using a Pre Heating Bath Cover A pre heating cover SciGene cat 1080 12 0 may be used to speed pre heating up to 95 C and reduce buffer evaporation on any bath type prior to slide processing R 4 BATH lt Ay ne 2 4 ES a Insert a preheating bath cover to achieve high temperatures and reduce evaporation Version 2 1 May 2015 13 408 733 7337 techserv scigene com Bath covers must be removed before starting any protocol unless the protocol includes a User Controlled Pause in the step prior to the bath with the protocol Couvercles de bain doivent tre enlev s avant de commencer tout protocole et ne peuvent pas tre utilis s pendant le fonctionnement E Using a Slotted Bath Cover for Prolonged High Temperature Immersion A slotted bath cover SciGene cat 1080 12 2 for 12 slide rack or 1080 12 4 for 24 slide rack maintains high temperatures up to 95 C and reduces buffer evaporation while a slide rack is immersed in a heated bath during a User Control Pause in the program See section V A Creating or Editing a Protocol for details on using an User Control Pause To use a slotted bath cover for pre heating and durin
29. wn 12 Centrifuge access panel 6 Low volume bath 13 Centrifuge latch 22 Stir bar 11a 12 slide rack fixed handle 11b 24 slide rack removable handle 5 Standard bath 21 Stir bar speed control 14 Centrifuge lock internal 1 Main power switch __ 20 Power cord connection 15 Touchscreen cable connection 19 Temperature calibration jack 10 Emergency switch 2 Bath power switch 23a Bath cover 3 Temperature controller 9 4 Temperature sensor 23b Slotted bath cover 16 Touchscreen NSS ne 17 Pause button Name on Bath cover 23a b Lids help achieve and maintain temperatures above 70 C Optional accessories Version 2 1 May 2015 10 408 733 7337 techserv scigene com Internal View of Centrifuge 11b 24 slide rack 24b Spacer for 24 slide rack 25b Absorbent pad large 18b Swinging bucket large 11a 12 slide rack 24a Spacer for 12 slide rack 25a Absorbent pad small 18a Swinging bucket small 13 Centrifuge latch Slide rack 11b Holds slides during processing Centrifuge latch 13 Keeps lid closed Centrifuge swinging buckets 18a b Green sample and red balance rack buckets Slide rack spacer 24a b Raises rack above absorbent pad Absorbent pad 25a b Captures liquid beneath slide rack during centrifuge spin Main Power Switch B Touch Screen and Power Connections 1 Connect the braided cable
30. ymbol indicates the ON position of the main POWER switch Ce symbole indique la position ON de l interrupteur principal Version 2 1 May 2015 3 408 733 7337 techserv scigene com 2 Non Electrical Symbols CAUTION This symbol illustrates a heat hazard Proceed with caution when A working around these areas to avoid being burned by hot components ATTENTION Ce symbole illustre un danger pour la chaleur Faire preuve de prudence lorsque vous travaillez autour de ces zones pour viter d tre br l par les composants du chauds CAUTION This symbol alerts you to consult this User s Manual for further information and to proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der avec prudence This symbol indicates the potential for a pinch point Do not place fingers in the slot in the Z axis tower Ce symbole indique la possibilit d un point de pincement Ne placez pas les doigts dans la fente dans la tour de l axe Z Biological hazards can be associated with the materials used on this instrument Potentially infectious waste and other hazardous waste must be treated in accordance with good laboratory practice guidelines Les dangers biologiques peuvent tre associ s des mat riaux utilis s dans cet instrument Potentiellement d chets infectieux et d autres d chets dangereux doivent tre trait s conform ment aux lignes directrices de bonnes
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