CMS_234011 - Applied Biosystems
Contents
1. 1 Verify that the printer contains paper Note Applied Biosystems recommends using continuous paper when printing from the 6100 PrepStation 2 While pressing and holding the Tear Off button turn on the printer m Tear Off Tear Off Load Eject Paper Out Er Pause m Draft m Draft Condensed m Roman m Roman Condensed m m Sans Serif m m Sans Serif Condensed W Micro Adjust 4 3sec Tear Off button The Epson LX 300 prints the language options for the printer 3 Press the Tear Off button again to select the default setting English The Epson LX 300 prints its current settings 4 Press the Tear Off button again to continue The Epson LX 300 prints instructions for configuring the printer settings 17 ABI PRISM 6100 Nucleic Acid PrepStation 18 To setup the printer 5 Configure the baud rate setting for the printer as follows a Press the Tear Off button 10 times 10x so that the first Font status light and the Pause status light are blinking see below m Tear Off m Draft m Draft Condensed m m Roman m Roman Condensed m m Sans Serif m m Sans Serif Condensed Tear Off Load Eject Paper Out ause W Micro Adjust ii 5 Font status light Blinking b Press the LF FF button until only the second Font status light is lit m Tear Off2. The 6100 PrepStation spools the appropriate data to HyperTerminal User Bulletin 15 ABI PRISM 6100 Nucleic Acid PrepStation Configuring the 6100 PrepStation to Print to a Printer Printing to a Dot Matrix Printer Printer Specifications Materials Required 16 Version 1 09 of the 6100 PrepStation firmware supports printing of run logs and methods directly to a dot matrix printer The 6100 PrepStation can download data to any printer that satisfies the minimum specifications listed below However for the purpose of explanation this user bulletin describes how to configure the 6100 PrepStation to print to an Epson LX 300 printer Another printer can be substituted as long as it meets the minimum requirements Printers for use with the 6100 PrepStation must e Be Dot matrix e Support ASCII or Esc P print formats e Support serial communication using the following parameters 9600 baud rate 8 data bits No parity stop bit No flow control e Epson LX 300 or comparable printer see the specification above e Dot matrix continuous paper e 9 Pin to 25 Pin Serial Communications Cable P N 4335209 see below 9 Pin DB9F ia 25 Pin DB25M Connector le Connector e DB9OF Connector 1 2 3 4 5 6 7 8 9 SHIELD DB25M Connector 8 3 2 20 7 6 4 5 22 SHIELD Figure 6 9 Pin to 25 Pin Serial Communications Cable P N 4335209 N
3. Draft m Draft Condensed m Roman m Roman Condensed Sans Serif ma Sans Serif Condensed Tear Off Load Eject Pause status light Blinking Paper Out Pause W Micro Adjust Second Font status light On Configure the parity setting for the printer as follows a Press the Tear Off button 1 time 1x so that both Font status lights first and second are blinking see below m Tear Off m MDraft m Draft Condensed Roman m Roman Condensed m Sans Serif m Sans Serif Condensed Tear Off Load Eject Paper Out Pause W Micro Adjust 4 3sec b Press the LF FF button until only the Pause status light is lit see below Font status lights Blinking Tear Off m Draft m Draft Condensed m Roman Roman Condensed Sans Serif m Sans Serif Condensed Tear Off Load Eject m Paper Out ause W Micro Adjust E Pause status light On User Bulletin User Bulletin Firmware Update and Printing from the 6100 PrepStation To setup the printer Printer on page 20
4. 3 Exit all open programs and applications that use the COM ports IMPORTANT The HyperTerminal connection requires one of the computer s two COM serial ports Before attempting the connection close all software using the COM ports to ensure an unobstructed connection IMPORTANT Some common laboratory software will not relinquish control of the COM ports even when closed In those cases you may need to use a different computer 4 Copy the HyperTerminal session files ht to the computer desktop as follows a Choose from the following If you Then downloaded the firmware update open the directory containing the from Applied Biosystems web site downloaded file from page 2 have the Firmware v1 09 CD 1 In the computer desktop double click the My Computer icon al lay My Computer Mw er Windows NT Windows 2000 2 Inthe My Computer dialog box double click the CD drive containing the Firmware v1 09 CD b Click and drag onto the desktop the following session files 6100 Printer COMI_A ht 6100 Printer COM2_B ht B 6100 Frinter 6100 Frinter COM Aht OMz_B ht The computer copies the HyperTerminal session files ht to the desktop User Bulletin 11 ABI PRISM 6100 Nucleic Acid PrepStation To configure the HyperTerminal program to communicate with the instrument 5 Double click the HyperTerminal session file that corresponds to the COM po
5. See Downgrading the Firmware below 6100 Utility Software Error No Response From the Instrument e Serial cable is unplugged or not seated properly e Designated COM port is Currently in use by another software application 1 Verify that the serial cable is connected to the firmware download port of the 6100 PrepStation see Figure 3 on page 3 and the correct COM port of the computer Reseat the serial cable connections Exit all programs using the COM port Return to the computer and click Yes U A W N If still unable to establish communication a Click No b Click OK c Exitthe 6100 Utility Software d Rebootthe computer and re attempt the download 6 If the problem persists call technical support IMPORTANT Some common laboratory software will not relinquish control of the COM ports even when closed In those cases use another computer Downgrading the Downgrading to an earlier version of firmware is not recommended However certain circumstances may require that the 6100 PrepStation run an earlier firmware IMPORTANT If the 6100 PrepStation firmware must be downgraded your users methods and preferences will be lost unless they are printed and then later restored To preserve your methods Before downgrading print all custom methods to either the HyperTerminal or a networked printer After downgrading recreate the methods manually Firmware
6. If you want to change these settings double click the Modems icon in Control Panel select this modem and click Properties Finish button HyperTerminal is now configured for use with the 6100 PrepStation COMMENT 12 Tech support had problems with this section To printa Then In the 6100 PrepStation main menu do one of the following Hyperterminal was method 1 Press Method F2 already set u 2 Inthe Method screen highlight a method of interest using the Hyperterminal would circular keypad and press More F4 neither show method Method User Steps LastUsed data or spool data from A method001 markb i SEA method002 markb 4 01 16 01 the 6100 although it method003 markb 5 01715701 looked as if t was v method004 markb 99 01 04 01 sending data Run New Edit More Fl F2 F3 F4 F5 When used COMI1 Bionic ner ean 3 In the Method screen press Print F3 blank FIyperiermimal Screen Method User Steps LastUsed A method001 markb LL Vo When used COM2 log method002 markb 4 01 16 01 method003 markb 5 01715701 data flowed by blank v method004 markb 99 01 04 01 hyperterminal screen Sort Delete GENRE More F1 F2 F3 F4 F5 log 1 Press Log F4 2 In the Log screen press Print F1 Run Quick Session Date Start 01 16 2001 M D Y Time Start 5 37pm 1 Collection Position v Setpoint 120 sec 50 Bee Clear SaveAs
7. parameters Verify and or Remedy 1 Verify that the serial cable is connected to the printer port of the 6100 PrepStation and the correct COM port of the printer 2 Verify and reseat the serial cable 3 If unable to establish communication 4 Using the documentation accompanying the printer verify that it is configured with the following communication parameters 9600 baud rate 8 data bits No parity lstop bit No flow control 5 If the problem persists call technical support 22 User Bulletin Firmware Update and Printing from the 6100 PrepStation 6100 Utility Software Troubleshooting Problem 6100 Utility Software Error No file selected Possible Causes Message occurs after selecting a file to download Verify and or Remedy Turn on the 6100 PrepStation power 6100 Utility Software Error Warning Application detected that your firmware methods are lost The methods were lost before the software attempted to download the new firmware Note The loss may be due to a hardware or system error e Download new firmware e Ifthe problem persists contact Technical Support 6100 Utility Software Error Warning You are trying to download an older version User developed methods will be deleted Are you SURE you want to do this Yes No The firmware version you are attempting to download is older than the version currently running on the 6100 PrepStation
8. version l 2 User Bulletin 23 ABI PRISM 6100 Nucleic Acid PrepStation Viewing Firmware The 6100 Utility Software can be used to view the firmware information from a Information networked 6100 PrepStation both before and after a firmware download To view firmware information 1 In the desktop double click the ABI6100 icon 2 Inthe Utility Application dialog box select File gt Get Firmware Info ABI Gremlin Utility Application Boot Version 00 X Method Version 01 2 Download Version 01 09 Checksum D995 Firmware Type 6100 3 View the information on the dialog box Field Explanation Boot Version number of the boot loader firmware currently running The boot Version loader firmware resides in a Flash ROM on the system board and is responsible for verifying the validity of the application firmware and if necessary restoring it from Flash or the firmware download serial port Method Version of methods that is running on the 6100 PrepStation It should Version always be the same as the download version unless there are problems in the system If this field is 00 00 then the methods are lost or corrupt Download Version number of the firmware application currently running This Version firmware is downloaded to the system through the firmware download Serial port If this field is the application is not available Checksum This checksum ser
9. menu ABI PRISM 6100 Nucleic Acid PrepStation To upgrade the firmware on the 6100 PrepStation continued 10 When the 6100 PrepStation displays the main menu see below return to the computer and click OK from the Completing Installation dialog box HH MM SS Applied Biosystems MM DD YY ABI PRISM 6100 PrepStation Version 01 09 Main menu User lt ABI gt Quick Method User Log F1 F2 F3 F4 ES 11 Confirm the completion of the download as follows a Verify that the instrument is displaying the main menu If so click Yes 7 Can you see the Main Menu on the instrument mo b Again verify that the instrument is displaying the main menu If so click Yes Si Can you see the Main Menu on the instrument No c In the Firmware Download Complete dialog box click OK SEG Download Complete downloaded firmware version is 01 09 0 oK 12 Return to the 6100 PrepStation The instrument should display the following screen Remote Control of 6100 Cycle power to restart in normal mode Wait 5 seconds before turning the power back on User Bulletin User Bulletin Firmware Update and Printing from the 6100 PrepStation To upgrade the firmware on the 6100 PrepStation continued 13 Turn off the 6100 PrepStation wait 5 sec then turn the power on The main menu appears displaying the new firmware vers
10. 7 Configure the data length setting for the printer as follows a Press the Tear Off button 1 time 1x so that first Font status light is lit and the Pause status light s blinking m Tear Off Tear Off Load Eject Paper Out ause m Draft m wm Draft Condensed m m Roman m Roman Condensed m m Sans Serif m um Sans Serif Condensed ii 5 W Micro Adjust Font status Pause status fight On light Blinking b Press the LF FF button until only the Pause status light is lit m Tear Off Tear Off Load Eject m Paper Out E E ause m MDraft m wm Draft Condensed m Roman m Roman Condensed m m Sans Serif m Sans Serif Condensed _ I m W Micro Adjust Pause status light On 8 Turn off the printer The Epson LX 300 printer is now configured for use with the 6100 PrepStation 9 Print to the installed printer as explained in Printing to a Dot Matrix 19 ABI PRISM 6100 Nucleic Acid PrepStation Printing to a To issue a print command from the 6100 PrepStation Dot Matrix Printer 20 1 Turn on the 6100 PrepStation and the attached printer or computer Note If printing to a printer verify that the printer contains paper Applied Biosystems recommend using continuous paper to print data from the 6100 PrepStation 2 Inthe 6100 PrepStation main menu choose one of the following HH MM SS Applied Biosystems MM DD YY ABI PRISM 6100 PrepStation Version 01 09 User lt ABI gt Quick M
11. Turn off the power wait 5 seconds then turn the power on again 3 If the problem persists change the fuses as explained in the ABI PRISM 6100 Nucleic Acid PrepStation Users Guide User Bulletin 21 ABI PRISM 6100 Nucleic Acid PrepStation HyperTerminal Software Troubleshooting Problem HyperTerminal Error Unable to open COM1 COM 2 Please check your port settings Possible Causes Serial cable is unplugged or not seated properly Designated COM portis Currently in use by another software application Verify and or Remedy 1 Verify that the serial cable is connected to the printer port of the 6100 PrepStation and the correct COM port of the computer 2 Reseat the serial cable connections 3 Exit all programs using the COM port 4 If unable to establish communication a Click OK b Exit the HyperTerminal program c Plug the serial cable into the alternate Serial port d Re attempt the connection using the alternate COM port 5 If the problem persists re attempt the procedure using another computer IMPORTANT Some common laboratory software will not relinquish control of the COM ports even when closed In those cases use another computer Printer Troubleshooting Problem 6100 PrepStation will not print to networked printer Possible Causes Serial cable is unplugged or not seated properly Printer is not configured with the correct communications
12. User Bulletin ABI PRismM 6100 Nucleic Acid PrepStation April 4 2002 SUBJECT Firmware Update and Printing from the 6100 PrepStation Version 1 09 Version 1 09 of the ABI PRISM 6100 Nucleic Acid PrepStation Firmware is now Firmware Now available from Applied Biosystems The new firmware features the following Available Support for printing run logs and methods to The HyperTerminal accessory of the Microsoft Windows operating system A dot matrix printer e A run log that reports error states and steps executed e Ability to create new methods from a run log session e Ability to view the calibration and configuration parameters of the 6100 e Increased program stability New Command The following figure illustrates the command pathways that access the features Summary provided by the new firmware Main Menu HH MM SS Applied Biosystems MM DD YY ABI PRISM M 6100 PrepStation Version 01 09 User lt ABI gt Utilities Menu Quick Method User Log Utilities Config Instrument Configuration Calib Calibration Verification Info Instrument Information Run Quick Session Date Start 01 16 2001 M D Y Time Start 5 37pm 1 Collection Position v Setpoint 120 sec 50 Instrument Configuration Set Time Print Clear SaveAs Mew Done Time 11 30 AM 12Hr Date 03 26 01 M D Y Run Time Sound OFF Date Cance New Method Calibration Verification Off
13. al cable is unplugged or not seated properly e Powerisnoton e Firmware has been lost due to a hardware system error 1 Verify and reseat the serial cable 2 Turn on the instrument power 6100 PrepStation Error Communication was broken during a download Serial cable was disconnected 1 Verify and reseat the serial cable 2 Turn off the power wait 5 seconds then turn the power on again 3 Re attempt the upgrade 6100 PrepStation Error Method version is 00 00 Methods on the 6100 PrepStation were lost 1 Download the firmware See page 2 2 If the problem persists call technical support Status Light in the rear of the 6100 PrepStation Solid Instrument is running normally Status light is blinking more frequently than 2 blinks second Firmware is downloading No action required IMPORTANT Do not turn the 6100 PrepStation power off during the installation Doing so will cause the instrument to lose all methods users and preferences Status light is blinking at the rate of 2 blinks second e Boot loader is running Firmware has been lost due to a hardware system error 1 Verify and reseat the serial cable 2 Turn off the power wait 5 seconds then turn the power on again 3 Re attempt the upgrade No light 6100 PrepStation is unplugged or a fuse has blown 1 Verify that the instrument is plugged into a working socket 2
14. eic Acid PrepStation Firmware v1 09 CD P N 4334652 Firmware v1 09 installer file from the Applied Biosystems Web site e Windows compatible computer that satisfies the following requirements Running Windows NT Service Pack 5 or later or Windows 2000 Contains a CD drive or has Internet access e 6100 PrepStation Serial Connection Cable P N 4330755 9 Pin DB9YF FEMALE Connector 9 Pin DB9YF FEMALE Connector Figure 2 6100 PrepStation Serial Connection Cable Note Both the serial connection cable and the Utility Software CD are included with the 6100 PrepStation IMPORTANT The 6100 firmware download does not work with Windows 98 The Version 1 09 Update can be ordered as a CD or downloaded from the Applied Biosystems Web site To Then order the Update CD 1 Contact a local Applied Biosystems Sales and Service Office 2 Order ABI PRISM 6100 Nucleic Acid PrepStation Firmware v1 09 CD P N 4334652 download the update from the Web site 1 Using a web browser open the following URL www appliedbiosystems com 6100 2 In the navigation bar click Downloads 3 Click Updates and Patches 4 Click the icon to download the installer file User Bulletin Connecting the 6100 PrepStation to the Computer Installing the 6100 Utility Software COMMENT Forcing the user to use another computer seems like a drastic step Is there any way they can c
15. ethod User Log To printa Then method 1 Press Method F2 2 Inthe Method screen highlight a method of interest using the circular keypad and press More F4 Method User Steps LastUsed 4 method001 markb Teale Wiley Alka ONE method002 markb 4 01 16 01 method003 markb Se 012718701 v method004 markb 99 01 04 01 Run New Edit a Fl F2 F3 F4 F5 3 In the Method screen press Print F3 Method User Steps LastUsed 4 method001 markb N RP method002 markb 4 01 16 01 method003 markb gt 041715701 v method004 markb 99 01 04 01 Sort Delete u More F1 F2 F3 F4 F5 log 1 Press Log F4 2 In the Log screen press Print F1 Run Quick Session Date Start 01 16 2001 M D Y Time Start 5 37pm 1 Collection Position v Setpoint 120 sec 50 mime Clear SaveAs The 6100 PrepStation spools the appropriate data to the printer User Bulletin Firmware Update and Printing from the 6100 PrepStation Troubleshooting Troubleshooting Index For problems with the See Page 6100 PrepStation and Firmware Troubleshooting 21 HyperTerminal Software Troubleshooting 22 Printer Troubleshooting 22 6100 Utility Software Troubleshooting 23 6100 PrepStation and Firmware Troubleshooting Problem Possible Causes Verify and or Remedy Startup Errors Problems Instrument does not respond during startup e Seri
16. from step 9 on page 4 d In the Edit menu select Paste The computer copies the prep0109 abs file into the ABI6100 Utility Software directory In the desktop double click the ABI6100 icon A In the ABI 6100 Utility Application dialog box click Choose File JABI 6100 Utility Application File Help 1 Select A Firmware File To Download Choose File button Choose File No Fite Seierted 2 Click To Start Bytes Remaining In the Choose Firmware Download File dialog box select the prep0109 abs firmware file as follows a Double click the Firmware folder icon b Click the prep0109 abs file to select it c Click Open E Choose Firmware Download File Look in C Firmware v al m fe i N prepo109 abs prep0109 abs file File name Open button Files of type The dialog box appears again with the selected file name displayed User Bulletin User Bulletin Firmware Update and Printing from the 6100 PrepStation To upgrade the firmware on the 6100 PrepStation continued 8 In the ABI 6100 Utility Application dialog box click the Start button ABI 6100 Utility Application File Help 1 Select A Firmware File To Download 2 Click To Start Choose File Bytes Remaining 370292 0 prep0109 abs Start button The 6100 Utility Software downloads the fir
17. heck to see if an programs are using the COM ports COMMENT Tech support noted that the icon for the Setup program was different than the one shown here I took this screenshot from the installer I was given Which is correct User Bulletin Firmware Update and Printing from the 6100 PrepStation To prepare the 6100 PrepStation and computer for the upgrade L Attach one end of the cable to the COM 1 port on your computer 2 Attach the other end of the cable to the firmware download serial port at the rear of the 6100 PrepStation as shown in Figure 3 below Firmware download port Figure 3 6100 PrepStation Firmware Download Port To prepare the 6100 PrepStation and computer for the upgrade l Turn on the 6100 PrepStation 2 Turn on the computer and login IMPORTANT You must have privileges to install software to proceed Exit all open programs and applications IMPORTANT The firmware download requires the use of the computer s COM 1 serial port Before attempting the upgrade close all software using the COM ports IMPORTANT Some common laboratory software will not relinquish control of the COM ports even when closed In those cases you may need to use a different computer Insert the Utility Software CD into the CD drive and close the drive door Run the Setup program on the Util
18. ied Biosystems InstallShield Cancel 11 Once the installation is complete click Done from the Install Complete dialog box The installer creates an ABI6100 Utility Software icon on the desktop EJ TL ABIE100 Utility Software 12 Remove the 6100 Utility Software CD from the drive and put it in a safe place User Bulletin Firmware Update and Printing from the 6100 PrepStation Upg rading the To upgrade the firmware on the 6100 PrepStation Firmware 1 Close all software running on the computer IMPORTANT The firmware download requires the use of the computer s COM 1 serial port Before attempting the upgrade close all software using the COM ports 2 Do one of the following e Insert the Firmware CD into the computer CD drive and close the door or e Download version 1 09 of the firmware from the Applied Biosystems web site www appliedbiosystems com 6100 Note See Acquiring the Firmware Update on page 2 for instructions on ordering or downloading the firmware update 3 Copy the firmware file as follows a Navigate to the prep0109 abs file as follows If upgrading the firmware from Then the installer file downloaded from open the directory containing the the Applied Biosystems web site downloaded file from page 2 see page 2 for instructions on downloading the firmware update the installer file downloaded from 1 In the computer desk
19. ine help for the HyperTerminal program or visit http www hilgraeve com e One of the following ABI PRISM 6100 Nucleic Acid PrepStation Firmware v1 09 CD P N 4334652 Firmware v1 09 installer file from the Applied Biosystems web site e Computer running Windows NT Service Pack 5 or later or Windows 2000 e 6100 PrepStation Serial Connection Cable P N 4330755 see Figure 2 on page 2 IMPORTANT If you have just completed the firmware download and have already linked the 6100 PrepStation to the computer go to Setting Up HyperTerminal on page 11 To connect the 6100 PrepStation to print to HyperTerminal 1 Attach one end of the serial cable to the COM 1 port of the computer 2 Attach the other end of the cable to the printer download serial port at the rear of the 6100 PrepStation as shown in Figure 5 below Printer port 6j Figure 5 6100 PrepStation Printer Port User Bulletin Firmware Update and Printing from the 6100 PrepStation Setting U P To configure the HyperTerminal program to communicate with the instrument HyperTerminal l If not already done perform the version 1 09 firmware upgrade as explained in Upgrading the 6100 PrepStation Firmware on page 2 2 Turn on the computer and login under a name that has administrative privileges IMPORTANT You must have privileges to install software to proceed
20. instrument 7 Depending on the operating system you are running choose from the following options Windows NT Windows 2000 a In the HyperTerminal dialog a In the Phone and Modem box click Yes Options dialog box click OK Phone And Modem Options 2x You need to install a modem before you can make a connection Dialing Rules Would you like to do this now gt er The list below displays the locations you have specified gt Select the location from which you are dialing Locations b Go to step 8 i Location 94404 New Edit Delete OK Cancel Apply HyperTerminal is now configured for use with the 6100 PrepStation b Go to step 12 8 Windows NT only In the Install New Modem dialog box select the Don t detect my modem check box and click Next Install New Modem Windows NT will now try to detect your modem Before continuing you should 1 If the modem is attached to your computer make sure it is turned on 2 Quit any programs that may be using the modem Click Next when you are ready to continue VW Don t detect my modem will select it from a list Don t detect my modem check box Next button ma 13 ABI PRISM 6100 Nucleic Acid PrepStation 14 To configure the HyperTerminal program to communicate with the instrument 9 Windows NT only Configure the Install Ne
21. ion HH MM SS Applied Biosystems MM DD YY ABI PRISM 6100 PrepStation Version 01 09 Firmware version Should be 1 09 User lt ABI gt Quick Method User Log F1 F2 F3 F4 F5 14 Return to the computer then select File gt Exit to close the 6100 Utility Software ABI PRISM 6100 Nucleic Acid PrepStation Configuring the 6100 PrepStation to Print to HyperTerminal About HyperTerminal Materials Required Connecting the 6100 PrepStation to the Computer 10 The 6100 PrepStation can be configured to print to the HyperTerminal program an accessory of the Microsoft Windows operating system HyperTerminal is a small communications utility that can be used to connect the computer to Internet telnet sites bulletin board systems BBSs online services and other computers through a modem or a null modem cable Once configured HyperTerminal records all data passed to and from the computer and the 6100 PrepStation and displays it within the program window The HyperTerminal data can then be printed cut and pasted into another application or saved as a HyperTerminal Session File ht for later use 2 Options e Print the data ona local or network gt printer e Cutand paste into a word processor or email utility 6100 PrepStation Hyperterminal Figure 4 HyperTerminal Download Process Note For more information on HyperTerminal see the onl
22. ity Software CD as follows a In the computer desktop double click the My Computer icon b In the My Computer dialog box double click the icon representing the CD drive containing the 6100 Utility Software CD oe amp My Computer My Computer Windows NT Windows 2000 E c In the CD drive dialog box double click the Setup icon setup exe The computer runs the InstallAnywhere program ABI PRISM 6100 Nucleic Acid PrepStation To prepare the 6100 PrepStation and computer for the upgrade 6 In the AB6100 Utility dialog box click Next gt In the Welcome dialog box click Next gt Read the release notes informat on and click Next gt In the Destination Folder dialog box do one of the following To install the software to Then the default folder 1 Click Next gt ne ommiendee 2 Go to step 6 below a different folder 1 Click Change 2 Using the Change Current Destination Folder dialog box select a directory for the installation 3 Click OK to accept the highlighted directory and return to the installation 4 Click Next gt 5 Go to step 6 below 10 Click Install The program installs the 6100 Utility Software to the designated directory Current Settings Setup Type Destination Folder C Program Files ABI6100 Installation User Information d i rec to ry Name Your Name Company Appl
23. l office or refer to our web site at www appliedbiosystems com www appliedbiosystems com AB A Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Applied Biosystems Printed in USA 04 2002 Part Number 4335337 Rev A Stock Number 117UB05 01 an Applera business
24. mware to the 6100 PrepStation Wait while the firmware completes the installation IMPORTANT Do not turn the power off to the 6100 PrepStation during the installation Doing so will cause the instrument to lose all methods users and preferences During the installation the following will occur Computer 6100 PrepStation The software displays the The status light at the rear of the installation progress by listing the 6100 PrepStation flashes green Bytes Remaining count down rapidly more frequently than re 2 blinks second AABI 6100 Utility Application lolx Q File Help 3 1 Select A Firmware File To Download a Choose File prep0109 abs 2 Click To Start D Bytes Remaining 279022 T 5 m Progress bar Bytes Remaining The computer displays the The 6100 PrepStation displays the Q Completing Installation dialog box Completing Installation screen fe Completing Installation Completing Installation s This may take several minut 8 e Please wait while the firmware completes the D NOP cycle the instrument installation power at this time This may take several minutes D DO NOT turn power off during this process c Click OK after the Main Menu on the instrument u appears iL When the installation is complete the instrument displays the main
25. ote The serial communications cable for use in this procedure can be constructed using the desired length of 28 9C serial cable and standard 9 Pin and 25 Pin connectors by following the pin connections shown above User Bulletin Firmware Update and Printing from the 6100 PrepStation Connecting the To connect the 6100 PrepStation to the printer 6100 PrepStation to the Printer Setting Up the Printer User Bulletin 1 Attach the 9 pin female connector to the printer serial port at the rear of the 6100 PrepStation as shown in Figure 5 on page 10 2 Attach the 25 pin male connector to the serial communications port of the printer 3 Choose one of the following options e If using an Epson LX 300 Printer see Setting Up the Epson LX 300 Printer below e If using a printer other than a Epson LX 300 follow the instructions in the accompanying documentation to configure the printer with the following communication parameters 9600 baud rate 8 data bits No parity stop bit No flow control IMPORTANT Complete the following procedure only if you are configuring an Epson LX 300 Epson LX 300 Printer for use with the 6100 PrepStation If using another printer follow the instructions in the documentation accompanying your printer to configure the printer with the parameters outlined in step 3 above To setup the printer
26. rt that is connecting the computer to the 6100 PrepStation e If the serial cable linking the computer to the 6100 PrepStation is connected to COM or COM A double click the 6100 Printer COMI A ht file If the serial cable linking the computer to the 6100 PrepStation is connected to COM 2 or COM B double click the 6100 Printer COM2_B ht file The computer launches the HyperTerminal software 6 IMPORTANT The location information entered in this step is inconsequential for communication with the 6100 PrepStation and will not be transmitted or used in any way during this procedure In the Location Information dialog box do the following a Click the country region drop down list select a location Location Information 2 xi Before you can make any phone or modem connections Windows needs the following information about your current location What country region are you in now United States of America Country region drop down list b Click the area code text field and type an area code What area code or city code are you in now Area code text field If you dial a number to access an outside line what is it The phone system at this location uses Tone dialing Pulse dialing c Click OK Er OK button 12 User Bulletin User Bulletin Firmware Update and Printing from the 6100 PrepStation To configure the HyperTerminal program to communicate with the
27. set Step Position Consult the user manual 1 Waste 120 Pressure Offset 20 Pressure Gain 98500 Save Insert Delete Save Reset Gain Cancel F5 Figure 1 New Feature Summary Within This This user bulletin discusses the following topics Bulletin Upgrading the 6100 PrepStation Firmware 0 000 cece eee eee 3 Configuring the 6100 PrepStation to Print to HyperTerminal 10 Configuring the 6100 PrepStation to Print to a Printer 15 Troubles hoon oe Lib oh oe aie ater bee ae sos bee ee beee a ose 20 gt Applied AB Biosystems ABI PRISM 6100 Nucleic Acid PrepStation Upgrading the 6100 PrepStation Firmware Aboutthe The firmware upgrade is accomplished in major three steps Upgrade Materials Required Acquiring the Firmware Update 1 Connecting the 6100 PrepStation to the computer 2 Installing the 6100 Utility Software to the computer 3 Downloading the new firmware to the 6100 PrepStation The upgrade takes approximately 15 minutes to accomplish and the entire procedure can be completed without turing off the computer or the instrument Note The following procedure is duplicated in the ABI PRISM 6100 Nucleic Acid PrepStation User Guide P N 4326242 See the user guide for detailed instructions e ABI PRISM 6100 Nucleic Acid PrepStation Utility Software CD P N 4331757 e One of the following ABI PRISM 6100 Nucl
28. top double click the Firmware v1 09 CD the My Computer icon see page 2 for instructions on ordering the CD 2 Inthe My Computer dialog box double click the CD drive containing the Firmware v1 09 CD b Click the file entitled prep0109 abs prepOiog abs c In the Edit menu select Copy User Bulletin 5 ABI PRISM 6100 Nucleic Acid PrepStation COMMENT Tech support noted that the ABI6100 directory was called the ABI 6100 Utility Software Is this correct COMMENT Tech support noted that the firmware file will be zipped if downloaded from the web Do we want to include instructions for using WinZip to extract the file COMMENT Tech support noted that the firmware file would not load Ist attempt would not open 2nd attempt Asked to select application to open the file 3rd attempt Worked fine To upgrade the firmware on the 6100 PrepStation continued 4 Paste the firmware file into the Firmware folder of the ABI6100 program directory as follows a In the computer desktop double click the My Computer icon b In the My Computer dialog box double click the drive C icon c Navigate to the Program Files gt ABI6100 gt Firmware folder 5 IC Program Files ABI6100 Firmware Note If drive C does not contain a Program Files gt ABI6100 folder navigate to the directory to which you installed the 6100 Utility Software
29. ves as a check for the application firmware to ensure that the correct firmware is running It is a double check in case the application version reported is misleading or incorrect If this field is blank the bootloader is running Firmware Type Firmware Type Explanation 6100 The 6100 PrepStation is running the firmware version indicated in the Download Version status BOOT The instrument firmware has been lost due to a hardware or system error Other indications of a failure to boot include blank screen on startup and status light at the 6100 PrepStation s rear blinks at the rate of 4 Click OK to return to the Utility Application dialog box 5 Return to the computer then select File gt Exit to close the software 24 User Bulletin Firmware Update and Printing from the 6100 PrepStation User Bulletin 25 ABI PRISM 6100 Nucleic Acid PrepStation 26 User Bulletin Firmware Update and Printing from the 6100 PrepStation User Bulletin 27 Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 Worldwide Sales and Support Applied Biosystems vast distribution and service network composed of highly trained Support and applications personnel reaches into 150 countries on six continents For sales office locations and technical support please call our loca
30. w Modem dialog box as follows a In the Manufacturers list box select Standard Modem Types b In the Models list box select Dial Up Networking Serial Cable between 2 PCs c Click Next Sa Click the manufacturer and model of your modem If your modem is not listed za orif you have an installation disk click Have Disk Manufacturers list box Models Standard Modem Types NEN Standard 300 bps Modem Standard 1200 bps Modem Standard 2400 bps Modem Models list box Standard 9600 bps Modem Standard 14400 bps Modem Standard 19200 bns Modem x Have Disk Cancel Next button 10 Windows NT only Select the COM port for the connection as follows a Select the Selected ports radio button b In the list box select the appropriate COM port for the connection c Click Next Install New Modem You have selected the following modem Dial Up Networking Serial Cable between 2 PCs On which ports do you want to install it All ports Selected ports Select ports radio button COM2 List box Cancel Next button User Bulletin Firmware Update and Printing from the 6100 PrepStation To configure the HyperTerminal program to communicate with the instrument 11 Windows NT only Click Finish Note The previous steps steps 8 to 8 are necessary only the first time the HyperTerminal application is run Install New Modem Your modem has been set up successfully
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