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O&M Manual for NuAire NU-540 BioSafety Cabinet

1. Sliding Window Assembly and Adjustment BCDz16299 dec Base Cabinet Assembly Electrical Schematics BCD 16517 sheet 1 and 2 NU 540 300 400 500 600 Electrical Schematic Page 4 of 61 LabGard ES Energy Saver Class Type A2 Laminar Flow Biological Safety Cabinet Models NU 540 300 400 500 600 Manufactured by NuAire Inc Plymouth Minnesota U S A 1 0 General Information 1 1 Description The LabGard ES Model NU 540 Laminar Flow Biological Safety Cabinet LFBSC is a bench table top model optionally available with a base support stand for operation as a console model The LabGard ES model NU 540 utilizes an Energy Saver DC ECM motor optimally determined forward curved fan for each model size width to maximize both energy efficiency and filter loading capacity The Energy Saver ECM motor is controlled by the Aeromax Control System to nominal airflow setpoints via an integrated solid state DC motor controller based on feedback algorithm to provide automatic compensation constant volume control for both filter loading and line voltage variances Airflow velocity is monitored by the PresurFlow system with a digital pressure sensor The Laminar Flow Biological Safety Cabinet LFBSC is a product resulting from the development of the laminar flow principle see Airflow Schematic and the application of environmental controls as required in the field of biological researc
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5. Bench top console Bench top console Bench top console Bench top console Style of Cabinet w base stand storage w base stand storage w base stand storage w base stand storage cabinet cabinet cabinet cabinet All welded stainless steel All welded stainless steel All welded stainless steel All welded stainless steel Cabinet Construction 16 18 GA Type 304 16 18 GA Type 304 16 18 GA Type 304 16 18 GA Type 304 pressure tight design pressure tight design pressure tight design pressure tight design HEPA Filter Seal Type Supply Filter 99 99 Eff on 0 3 microns HEPEX Seal HEPEX Seal HEPEX Seal HEPEX Seal Exhaust Filter 99 99 Eff on 0 3 microns Neoprene Spring loaded Neoprene Spring loaded Neoprene Spring loaded Neoprene Spring loaded Standard Services Service Coupling 3 8 inch NPT One Right Sidewall One Right Sidewall One Right Sidewall One Right Sidewall Service Coupling 3 8 inch NPT Two Left Sidewall Two Left Sidewall Two Left Sidewall Two Left Sidewall Gas Valve Service Coupling 3 8inch NPT One Right Sidewall One Right Sidewall One Right Sidewall One Right Sidewall Duplex Outlet Two Backwall Two Backwall Two Backwall Two Backwall Optional Services Gas Cocks 3 8 NPT Up to ea Sidewall Up to 3 ea Sidewall Up to 3 ea Sidewall Up to 3 ea Sidewall Ultraviolet Light One Backwall One Backwall One Backwall One Backwall Standard Cup Sinks Left or Right Work Left or Right Work Left or Right Work Left or Right Work Surface Surface
6. Run Mode Any time the blower run key is pressed with the sliding window at its correct operational height the RUN MODE screen will be initiated The Run Mode will start with the PresurFlow entering and approximate 3 minute warm up period The PresurFlow LED indicators will blink and indicate the following sequence 1 minute Left and right Red LED s will blink e 2 minute Left and right Green LED s will blink e 3 minute Center 3 Green LED s will blink Once the warm up period is complete only one LED will indicate cabinet airflow status During the warm up period the aseptic cleaning process may begin If the sliding window is raised an audible and visual alarm will occur but may be silenced by pressing the alarm silence key Nite Care Mode The NU 540 may be configured to allow the DC ECM motor to continue to run at a lower rate with the sliding window closed allowing the workzone interior to be continually HEPA filtered If the Nite Care mode is configured the blower must be on green LED above blower key will blink and the window closed for it to be activated Once activated blower LED indicator will blink fast and the PresurFlow will indicate 3 Green LED indicators will blink In addition the fluorescent light will be disabled The UV light may be turned on if installed Standby Run Mode Alarms If present standby run mode alarms will be both visual and audible the red alarm LED oval will turn on Audible al
7. 0 2 LAN 02 V Vb 53015 13 18 3 NIVW dVMIS 411495 OL 35007 A3Hl OS 58419 31892 S30123NN02 7991519373 123 02510 VE 9 9 DVH dHV12 ISOH 9 9 MIA KN3S3Nd JD 3SOH 390 9 OLI3HINIM 3ADWGN SavMIS 113495 340 331N32 71051909 137 41139 ONY 8 32 71081409 40 401 033 543425 86 88 2 9 118915 380338 LINN DL 21 435 21819313 TW 123NNDOSIG N Tinv3 3404039034 31435 7031403 51 1 21 6 ni d3ssv3T33 tTel v 3190 15182530 A33 1 353 31 119207 391099 2 335 57 61 0 0217 October 2014 9 91 140 133 5 s IN3WiSnr v 8 ASSY ADONIM 9 10115 0 5 252525252525252525 525252525252525 525252525252525252525252525252525 COO COOOOOoGocoooocooocooccoococoocococooooooococ CIVNDILdCOD AD 3840 JLN MO 330 HAJAD HILIMS 035070 MDUNIM Wavy HOLIMS HOIH ADGNIA JONY ADNIA ONIGIIS 7103 1291 02 3398 711158 S3H21IAS AOONIM ASTSSA 31374803 SI iN3WiSnr v JINO NOLLVALSNTIL NO CALYIIANI 59 INYA 11930034 GNIH38 401 ONY 431 30 7103109 IHL GNIH3H 310018 01100 SLOAN 02 b 1 3 35 01 SI ATEW3SSY 4
8. 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within 20 or 16fpm 0 08m s whichever is greater factory test or 25 or 16fpm 0 08m s whichever is greater field test from the average downflow velocity B Inflow Measurement a Recommended Instrument Shortridge Flowhood ADM 870 or TSI 8355 Thermo anemometer b Primary Procedure The primary procedure to determine inflow velocity uses a Direct Inflow Measurement DIM Instrument i e Shortridge flowhood The DIM Instrument can be used directly on the cabinet with NO CORRECTION FACTORS REQUIRED if operated in the local density default mode NSF has tested the cabinet and established listed air velocities expressed in local density The DIM Instrument should also be duct taped to the cabinet to prevent any sneak air paths from occurring The DIM Instrument will read inflow volume i e CFM Use the window access opening area to calculate inflow velocity 0217 Page 40 of 61 October 2014 Alternate Procedure The alternative procedure to determine inflow velocity uses a thermo anemometer in a constricted window access opening of 3 inches 76mm with the armrest removed Inflow air velocity is measured in the center of the constricted opening 1 1 2 inches 38mm blow the top of the work access opening on the following specified grid Use the correction factor table to calculate the inflow velocity Test Data Inches 1 Dim Measurement Inflow V
9. Oospora lactis Yeasts Saccharomyces ellipsoideus Saccharomyces cerevisiae Brewers yeast Baker s yeast Common yeast cake Bacteria Streptococcus lactis Strep hermolyticus alpha type Staphylococcus aureus Staphylococcus albus Micrococcus sphaeroides Sarcina lutea Pseudomonas fluorescens Escherichia coli Proteus vulgaris Serratia marcescens Bacillus subtilis Bacillus subtilis spores Spirillum rubrum Microwatt seconds cm 2 26 400 22 000 88 000 88 000 99 000 330 000 220 000 35 200 35 200 11 000 13 200 17 600 13 200 6 600 8 800 13 200 8 800 5 500 6 600 5 720 15 400 26 400 7 040 7 040 7 480 6 160 11 000 22 000 6 160 Protozoa Paramecium Nematode Eggs Algae Virus Baceriophage E Coli Tobacco Masaic Influenze a Luckiesh Matthew 1946 Application of Germicidal Ethyemal and Infrared Energy D Van Nostrand o New York New York pp 253 b Hollaender 1942 Aerobiology A A A S for 9096 inactivation pp 162 c Ellis C and Wells O O 1941 The Chemical Action of Ultraviolet Rays Reinhold Publishing Corp pp 713 714 d Hollaender A Oliphant J W 1944 The inactivation effect of monochromatic ultraviolet Radiation on Influenze Virus for 9096 inactivation Jour of Bact 48 pp 447 454 e Thistable Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation s comes from Westinghouse brochure entitled Westinghouse Sterilamp Germicidal Ult
10. depressing the gasket material by 1 8 inch 3mm attention to the correct electrical connections see Electrical Schematic c Re install the new motor blower assembly Page 33 of 61 7 4 Sliding Window Replacement and Manual Adjustment The sliding window replacement is accomplished by removing the front decorative panel control center and window glide assemblies The sliding window adjustment may be required due to everyday use over the life of the cabinet Both window glides are adjustable by a set screw and tension screw method When adjusting the sliding window be sure to verify proper micro switch operation If the sliding window is too loose it will not properly activate the micro switches thus causing potential operational malfunctions to occur If the window counter balance experiences a fault window function must be verified the sliding window retention or ability to slow the rate of fall See Sliding Window Retention Verification in the Inspection Report 7 5 Airflow Control System Setup and Calibration 7 5 1 General The operation of the NU 540 cabinet requires that the setup and calibration procedures be performed in order to certify or commission the cabinet for usage The setup and calibration procedures performed ONLY BY THE CABINET CERTIFIER ensure that cabinet s setpoints are verified and that the airflow monitor sensor is calibrated to the correct values 7 5 2 Configuration Parameters Configuration parameters identify
11. or that personnel leave the cabinet face area Operation The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch located on the front panel The sliding window is interlocked to the ultraviolet light so when the sliding window is raised the ultraviolet light will turn off If operational time duration is known the timer can be used in conjunction with the ultraviolet light to time out the ultraviolet light operation This can be accomplished by first turning on the ultraviolet light Then set the timer to the desired length of ultraviolet light operation time Upon timer expiration the ultraviolet light will turn off Precaution The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin if of sufficiently high intensity or if exposure covers a prolonged period of time For this reason one should avoid direct eye and skin exposure to ultraviolet light If exposure cannot be avoided it is necessary for personnel to wear eye goggles or face shields and long sleeve gowns with rubber gloves Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the cabinet face area Page 51 of 61 10 1 4 Maintenance The output of an ultraviolet light deteriorates with burning age The useful life of the light is appr
12. A careful check of grills and diffuser grids should be made for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that contaminates the protected work environment 6 3 7 Paper Catch Prefilter A permanent paper catch is installed behind the rear divider panel of the work zone This area forms the return air path to the motor blower and if the airflow is blocked it could seriously affect the performance of the cabinet Therefore THE PAPER CATCH SHOULD BE CHECKED AND CLEANED ON A MINIMUM OF A WEEKLY BASIS A daily basis if procedures dictate the use of paper products Any paper removed must be properly disposed of as Contaminated Hazardous Waste The above procedures also apply to all cabinets configured with a prefilter 6 3 8 Shut Down Turn off blowers and lights Do not use cabinet as a depository for excess lab equipment during periods of non operation If antineoplastic agents are being prepared in the cabinet it is recommended to let the cabinet run 24 hours per day This lessens the possibility that contaminants may escape Ergonomics Ergonomics the study or accommodation of work practices is extremely important for proper cabinet usage and user health and safety An evaluation of normal work practices should be performed with each user when working in a cabinet Evaluation criteria should be at a minimum a Proper user posture b Effective work zone layout for work practice c Vision or sight
13. INTERUPT PRESURFLOW VN MONITOR ALARM Z COM SERIAL COM OUTPUT INTERFACE VIA RS 232 USB XR T SWITCHING 85 250 VAC POWER 12 SUPPLY 5VDC AEROMAX CONTROL SYSTEM BLOCK DIAGRAM 0217 October 2014 Figure Page 19 of 61 12991 099 ann T3NVd 1NO34 075 MI 2 aa st ava 7 023 20 61 0 0217 October 2014 6 1 2 0217 October 2014 Front Panel The control system front panel contains the following functions described in detail see Drawing BCD 16521 6 1 2 1 6 1 2 2 6 1 2 3 6 1 2 4 6 1 2 5 6 1 2 6 6 1 2 7 6 1 2 8 Blower Keys The blower key controls the ON OFF power to the blower LED above key indicates full green for blower on blinking green for blower pending and full red for blower alarm Hidden Key The hidden key is located just above the blower LED indicator centered in the airflow symbol The hidden key is used for various functions including the blower password 3 key sequence if the option is activated Fluorescent Light Key The fluorescent light key controls the on off power to the fluorescent light LED above the key indicates full blue for fluorescent light on Ultraviolet UV Light Key The UV light key controls the on off power to the UV light if optionally installed LED above indicates full yellow for UV
14. any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to this manual In case this manual and or test report is lost or misplaced NuAire retains a copy in our files A replacement copy can be obtained by calling or writing NuAire Inc stating the model number and serial number and a brief description of the information desired 0 0217 Page 3 of 61 October 2014 0217 October 2014 LabGard ES Energy Saver Class Type A2 Laminar Flow Biological Safety Cabinet Models NU 540 300 400 500 600 Operation and Maintenance Manual Table of Contents Sectl n NO 1 tac Rn eene General Information Section 2 Models and Features Section ere ae te ehe en enin Warranty Section Shipments Section 5 Installation Instructions Location 5552 5 52 222 20 0 3 Set up Instructions 583 Testing Methods and Equipment Section 6 Operating the NU 540 Aeromax Control System Operating Guidelines Operating Sequence tbe Ergonomics Cleaning Procedures SECTION NO General Maintenance Hes Decontamination ens Fl
15. bags and labeled for disposal as chemotherapy waste after completion of the procedure For the purpose of this procedure detailed procedures for cleaning a Class LFBSC can be found in the 2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal blower on b Open the hinged or sliding view screen and secure in the full open position With the view screen in the full open position personnel protection is compromised and a full faced HEPA filtered respirator must be worn c Clean all readily accessible surfaces of the cabinet Remove perforated metal diffuser screen from the underside of the supply HEPA filter and place the cabinet work tray Note Depending the model the diffuser screen is secured to the cabinet by 8 32 screws or 1 4 20 acorn nuts 3 places It is purposely a tight fit and is secured to the back wall with projecting threadless studs e Clean both sides of the perforated metal diffuser screen and remove it from the cabinet f the cabinetwork tray clean both sides and remove it from the cabinet g Remove the front perforated grill place on the cabinet floor and clean both sides Remove from cabinet h Clean work tray supports i Working from top to bottom clean all inside surfaces of the cabinet Take care not to wet the HEPA filter If liquid ha
16. cubic meter ppcm or 100 particles per cubic feet ppcf 1 USP28 NF23 United Stated Pharmacopeial Convention Inc 12601 Twinbrook Parkway Rockville MD 20852 USA www usp org 2150 14644 1 1999 Cleanrooms and Associated Controlled Environments Classification of Air Cleanliness International Organization for Standardization Case Postale 56 CH 1211 Geneve 20 Switzerland 3 CAG 002 2006 Compounding Isolator Testing Guide Controlled Environment Testing Association 1500 Sunday Drive Suite 102 Raleigh NC 27607 USA www cetainternational org Page 44 of 61 October 2014 7 10 Main Control Board Description and Replacement To access the main control board for fuse or board replacement remove screws at each upper side of the control center and allow the control center to rotate down resting on the safety straps Now the main control board is exposed for service 7 10 1 7 10 2 7 10 3 0217 October 2014 Main Control Board Replacement The main control board consists of one Printed Circuit Board PCB assembly The PCB contains the power supply configuration switch sensor inputs outputs and control inputs outputs components and display Main Control Board Fuse Replacement Disconnect electrical power from cabinet before fuse replacement All AC circuits are fuse protected and when replacement is necessary USE ONLY FUSES OF SAME TYPE AND RATING FOR PROTECTION AGAINST RISK OF FIRE BLOWER FUSE
17. from right side Be sure to note the location of the supply and exhaust sensor wires before disconnecting them from the main board Remove control center by disconnecting safety straps lifting the control center up and away from cabinet Fold and pinch tubing to seal Remove the front decorative panel via top front fasteners Remove window assembly both frame and window either being manual sliding or automatic via the upper and lower fasteners Refer to BCD 16376 At this point the sliding window assembly can be removed Remove exhaust sensor shrouds Remove the diffuser screen and gasket around perimeter of workzone if present Prepare decontamination equipment Reference decontamination procedure and use the following chart to calculate chemical requirements Cabinet Size 300 400 500 600 Cabinet 58 7 8 x 28 x 34 3 8 in 58 7 8 x 28 x 46 3 8 58 7 8 x 28 x 58 3 8 58 7 8 x 28 x 70 3 8 Dimensions 1 50 x 711 x 873 m 1 50 x 711 x 1 18 m 1 50 x 711 x 1 48m 1 50 x 739 x 1 88 m Cabinet 32 79 cu ft 44 24 cu ft 55 69 cu ft 67 14 cu ft Volume 929 cu m 1 25 cu m 1 58 cu m 1 90 cu m 10 lt Note The outlets in the work area are energized as long as the cabinet is plugged in and switched on the front panel Unplug the cabinet before decontamination equipment is plugged into these outlets The control centers electrical connectors may be re attached to utilize the cabinet s in
18. left maximum right blower speed has been achieved Note At any time during the process e Press hidden key to accept Nite Care blower speed calibration point e Press key to abort and exit 0217 Page 39 of 61 October 2014 Table 7 0 Recommended Measurement Methods for Cabinet Downflow amp Inflow A Downflow Measurement a Recommended Instruments TSI 8355 Thermo anemometer b Procedure Supply filter efflux is measured on a grid in a horizontal plane 4 inches 102mm above the bottom edge of the window No reading should be taken closer than 6 inches 152mm from the inside perimeter Test Data Inches Model Size Window Access Height 300 6 25 13 533 20 816 28 100 10 8 159 344 529 714 400 7 0 12 396 17 792 23 188 28 584 33 980 39 375 10 8 178 315 452 589 726 863 1000 500 7 25 13 518 19 786 26 054 32 322 38 590 44 858 51 125 10 8 184 343 503 662 821 980 1139 1229 600 7 5 15 410 23 320 31 230 39 140 47 050 54 960 62 857 10 8 191 391 592 793 994 1195 1396 1597 6 25 7 0 7 250 7 5 159 178 184 191 12 5 12 5 12 5 12 5 318 318 318 318 18 0 17 750 17 5 476 457 451 445 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity
19. level of the work opening This presents an inclined plane from hands to elbows along which the downflow of air may run to and possibly out the open face When working with agents of lower risk it is not as important for all materials to be placed in the cabinet before starting or for the procedure to be completely finished before materials are removed Also the time period for the cabinet may be continued over a longer extended period during which entries and withdrawals from the cabinet may be made Minimize Room Activity Activity in the room itself should be held to a minimum Unnecessary activity may create disruptive air currents as well as interfere with the work of the operator A person walking past the front of a cabinet can cause draft velocities up to 175 fpm 89 m s which are sufficient to disrupt the air balance of the LFBSC Page 25 of 61 6 2 4 6 2 5 0217 October 2014 Utilize Unidirectional Air Flow The operator must keep two important facts in mind 1 The air as supplied to the work area through filters from the top is contaminant free and 2 Airborne contamination generated in the work area is controlled by the unidirectional flow of parallel air streams in a top to bottom direction A solid object placed in a laminar air stream will disrupt the parallel flow and consequently the capability of controlling lateral movement of airborne particulates A cone of turbulence extends below the object and lamin
20. monitoring the exhaust flow to check position turning the choke adjustment shaft clockwise will open the choke while turning counter clockwise closed the choke Step 2 Once exhaust adjustment is complete return to the downflow calibration process and measure average downflow velocity If the downflow average remains within the correct range the calibration is complete Press hidden key to accept the blower speed calibration point If not readjust as necessary to obtain the correct calibration range Once entire cabinet has been balanced tighten liquid tight fastener around choke adjustment shaft Note Upon exit from the active blower speed adjustment the PresurFlow will be calibrated to the center green LED indicator High low alarm limits will be based on the default values established from the calibration point 0217 Page 37 of 61 October 2014 7 5 3 3 PresurFlow Alarm Set Points The PresurFlow alarm setpoints are preset based on the calibration setpoint Once the calibration setpoint is entered based on a nominal inflow velocity of 105fpm 53mls the associated pressure sensor value is entered as the nominal pressure value The high and low alarm setpoints are preset with an associated pressure offset value within the acceptable cabinet airflow range Normally no further adjustment is needed However if specific use alarm setpoints are desired the alarm setpoints may be adjusted by performing the following Low Ala
21. please perform the following Step 1 NOTE ALL ERROR MESSAGES Error message will appear on the control panel with red LED s Step 2 VERIFY ERROR MESSAGES Error messages can be verified by clearing the error function by either turning the blower or the cabinet on and off Step 3 MONITOR RE OCCURRENCE OF ERROR MESSAGES If re occurrence of the error message is immediate or daily use the following guide to correct the situation 0217 October 2014 Page 46 of 61 8 1 Error Message Troubleshooting Guide Error Message Error Description Correction Window Alarm Window High Sliding window is above standard working height or micro switch is not operating properly Verify standard working height and window micro switch operation Window Alarm Window Low Window Closed Sliding window is below standard working height or micro switch is not operating properly Indicates the window is in the full down position Verify standard working height and window micro switch operation Cabinet fluorescent lights won t Turn on Blue LED above light key indicates the lamp should be on Check light fuse on main control board Check fluorescent lamps Check voltage coming out of main control board to light ballasts Check light starters if present Check ballast Cabinet blower won t turn on Green LED above blower key indicates the blower should be on Airflow Alarm Check blower fuse on m
22. test e Airflow smoke patterns e Site installation assessment tests The testing methods and equipment required are specified on the factory inspection report included with this manual see insert in back cover NOTE IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR TESTING BIOLOGICAL SAFETY CABINETS SEE INSERT NOTE AFTER THE INITIAL CERTIFICATION NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT MINIMUM OF AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE MAINTENANCE ACTION OR ANY TIME THE OPERATOR FEELS IT IS NECESSARY Note that the LabGard ES cabinets filters and seals provide premium performance Quality Control in both design and manufacturing assure superior reliability However protection to both product and operator is so vital that installation testing to the performance requirements should be accomplished as stated to ensure biological safety established by the factory standards Page 17 of 61 October 2014 LabGard ES Energy Saver Class Type A2 Laminar Flow Biological Safety Cabinet Models NU 540 300 400 500 600 Catalog Number Catalog Number NU 540 300 NU 540 400 NU 540 500 NU 540 600 Nominal 3 foot 0 9m Nominal 4 foot 1 2m Nominal 5 foot 1 5m Nominal 6 foot 1 8m Pes coh 1 Personal Protection 2 Product Protection NSF ANSI 49 Class Type A2 Class Type A2 Class Type A2 Class Type A2
23. valve body until secure with the valve stem for handle on the left side Re install handle to valve stem Adjust the cabinet on bench to provide a 2 51mm overhang and seal the interface of the bench and cabinet using RTV caulk as above Gas Service NuAire doesn t recommend the use of natural gas within the LFBSC but if gas service is determined to be necessary for the application appropriate safety measures must take place All NuAire LFBSC s have precautionary warning labels that say the following Use of explosive or flammable substances in this cabinet should be evaluated by your appropriate safety personnel Once the determination has been made by the appropriate safety personnel the application of natural gas must be performed in accordance to national state and local codes IT IS ALSO STRONGLY RECOMMENDED THAT AN EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE All NuAire s LFBSC s meet the safety requirements of UL and CSA for Laboratory Equipment To comply with these safety requirements NuAire uses only certified gas valves If external piping is required only black pipe is used for this application As previously stated NuAire doesn t recommend the use of natural gas within the LFBSC and ASSUMES NO RESPONSIBILITY FOR ITS USE USE AT YOUR OWN RISK The Bunsen burner flame within the LFBSC not only contributes to heat build up it also disrupts the laminar air stream which must be maint
24. 05 0 1 1 27 2 54mm 0 05 0 1 1 27 2 54mm 0 05 0 1 1 27 2 54mm 0 05 0 1 1 27 2 54mm H20 H20 H20 H20 Heat Rejected BTU Per Hour 10 254 8 203 opening 10 254 8 203 opening 10 254 8 203 opening 10 254 8 203 opening non vented 903 826 1140 1020 1768 1611 1884 1768 vented 120 157 198 198 Electrical U L U L C Listed U L U L C Listed U L U L C Listed U L U L C Listed Volts AC 60 Hz 115 115 115 115 Amps Blower Lights 10 8 openings 2 3 2 1 2 9 2 6 4 5 4 1 4 8 4 5 Amps Duplex 3 3 3 3 Rated Amps 10 12 14 12 14 12 12 ft Power Cord one 14 GA 3 Wire 15A 14 GA 3 Wire 15A 12 14 GA 3 Wire 20A 12 14 GA 3 Wire 20A Std 15A Optional Std 15A Optional Net Weight 400 Ibs 181 kg 465 Ibs 211 kg 550 Ibs 249 kg 620 Ibs 281 kg 15A Configuration does not include accessory outlet Crated shipping weight does not include weight for accessories or options Based on cabinet with new filters running at 115VAC 0217 Page 18 of 61 October 2014 6 0 Operating the NU 540 6 1 Aeromax Control System 6 1 1 Overview The Aeromax control system is designed to service the control requirements of the LabGard ES NU 540 Biological Safety Cabinet The Aeromax control system consists of an electronic module that will perform the following functions Easy user interface via LED s and function keys Control blower via solid state switch Control lights via solid state switch Control o
25. 1 931 937 6 40253131 HSHSWM 1914 155 H3HSVM 4901 1001 9 1 y 1708 155 005 X 02 NI Q3T VISNI LYASLON 08 91 H3HSVM 114 LSS 71 3901 1 9 1 1709 LSS 005 X OZ v t NO 50115 OL LAN LSS 022 1 H3HSVM 201 HLOOL 1 1 13 LSS 9 1 2 L3 0v 8 ONILNNOW TWSYSAINN dg 51 0 01035 18 905 v miHo Widd 5 023 Page 56 of 61 October 2014 0 0217 V 0 91 004 2 1130 1401 eveve 87 wLa 3 11980330 14043 N31N32 7081407 EHS 1548 TYNIDING 54315 3ADHV 3543 34 T3NVd 3 11940230 119 01 CHE LN3S3Nd 13 30 18084 SA3NOS TITAN 9 3AOW3N 119130 13 30 3903 01 WD33 SLNN lt gt 3ADW33 549415 113395 N3dU 331N32 70 1 02 131 ONY 331N32 1081905 30 901 043 SA3HJS 26 88 2 3AUW3B VT 34132084 713 4 3AILVNDO3U AND 413495 554315 3AD8V 3S83A38 N31N32 1 1 2 H2VliV Dl 9 AWAY ONY dn 331832 7091909 1311 SONI 4 15 13495 1 S3SUH 39NV9 2IT3HINIW 831N32
26. 4 ACCESSORY OUTPUT FUSE FUSE TYPE TIME LAG TIME LAG TIME LAG TIME LAG FUSE SIZE 5 X 20MM 5 X 20MM 5 X 20MM NU 540 300 10 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 400 10 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 500 12 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 600 12 AMPS 3 AMPS 2 AMPS 1 AMP 2 Main Control Board Replacement Note All setup and calibration data will be lost the memory reinitialized to the default values and all control functions reset to an initial cabinet power condition If possible before the main control board replacement it would be preferred to know the operational parameters of the cabinet i e blower speed PWM signal DC voltage setpoints if modified and airflow data from previous certification Disconnect electrical power from the cabinet before attempting any maintenance action The main control board is fastened to the control center with 6 6 32 studs nuts All electrical connections are made with removable terminals and or Faston connectors except for the motor blower connector which uses a screw terminal Remove all electrical connections and fasteners and then remove the main control board from the control center Install new main control board by reattaching all electrical connections and fasteners Once installed rotate control center to normal position and fasten in place Reconnect power to cabinet Page 45 of 61 7 10 4 Aeromax Control System Reset The Aeromax control board has a reset functio
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28. DC output to turn on 8 2 2 Blower Airflow Options 0217 October 2014 To access the option parameter menu perform the following Press and hold key then press hidden and JJ keys sequentially Red LED indicator above the blower key will blink fast The PresurFlow blinking green LED segments will indicate seven optional parameters as shown and described below The UV Light key moves left and outlet key moves right allows selection of the option parameter desired Once the desired option parameter is indicated press or 4 key to turn on or off A slow blinking green LED indicator means off and a fast blinking green LED indicator means on Multiple option changes can be selected e Pressing the hidden key will accept all changes and exit e Pressing the E key will abort the process and exit 3 48 61 light 0217 October 2014 1101 Require Password Normally it is not required to use a password i e 3 key press sequence of the blower and hidden key If the option is turned on it would be required to use the correct password to turn on the blower The default password once turned on is blower hidden blower keys in sequence The password can be changed in the blower password option menu Allow UV light anytime Normally the UV light is interlocked with the window being in the closed position For service purposes only if the function is active the UV light may
29. Flow Biological Safety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m 0 0217 8 61 October 2014 8 9 15385148 81 1 2 134 5 1 219010 8 H3AVS 3dAL ll 55910 00 0 6 14683 SNOIS3WIO TW 39NV83101 TWNOISNAWIG wee 9490553181 ONVLS 35 8 TWNOUdO 131100 33 35 1432 1 SNndn09 2 5 039914 ONIOITS SOL SONSVIO WN31SAS HOLMS HOLIMS TOULNOD 31545 10 1 02 MO13HnS33d WALSAS TOHLNOO TANVd 1054405 SNIONY TVNHSANI Ti 860 21264908 CAE fotl vies SH313A31 931 38N 19 a tozla 158 195201 1H9I3H 3NOZWHOM 155 3 18V3 OW3M 4N3983H0 f14 14408 _ 9 x x 1695112 LSMWHX vd3H lesta x oselps x feocloz 5 1800 229v 3NOZWHOM 1899 cavi vio 8188 MVB 4HOTAN 3A OT8 HOLON
30. HLO TY 1669 9 15 3101 TVNOISNANNIG TIVH3AO sia uno SATA 321A43S 1 35 835 9359074 SNIGI S SOILSONSYIC 43155 HOLIMS HOLIMS LHOIT W3LSAS 1081 XVINOMN3V 1OH1NOO SNISNV IVE THHS 3 0 182 gl i lez o Fas 014041 44 ava 2530 093 INaWLSNray 5431331991 354 15 01 1829 3NOZWHOM 8 155 89 314405 Vd3H ladle 12201195 x 9912 TANVd 1NOH3 ANNOW HSM 150 Vd3H 128119 x 29408 x 180902 34018 ONIOVES 8 19 1413 HOLVO QL EL Q31vuOdH3d 8007 9291 Page 11 of 61 0 0217 October 2014 9 91891 02998 erozieuzi 51 JANISVD 134 5 1V219010 8 A9YIN3 11 55415 009 0 5 VNIOINO 35 8 G L SNOIS3WIG TY 18 9 SONVHAIOL 7IVNOISN3WIQ 17 12 51 5 143112 SNMANOD 39445 q399n
31. IHL 1341530 59 ANANIA 9 10115 JHL 110143 5531 TIM 100 AO ATHW3SSV 91593 ONIAGW 9 115 09 41883559 3 ADNIA 1331 LHOTd ONIAGW A8 3 511 339 SI 1 30119 MOONIA INSWISDCQV 30710 ADINIA da 51 t st or 01 13593138 121 v 1440 141435301 093 A33 Page 58 of 61 October 2014 0 0217 V 66291 0048 130 1 1338s 48 1 21 6 51 ATAWSSSY 13 1892 3594 0 5 914 138100384 SV ALO MAXIS HOVW NVd 26 88 13410934 SV 410 ONIN 1 35 26 88 1809 55322 15 3 0H23 150 v T 1 E LNN 02 1 43503 10 WON31d LNSNVWa3d WANITA C3LYNINYINOS nonya W 43123 02 4D 1404 39055344 ONYE AVaL 305 554239 431113 5 1 355 8431114 Vd3H 180 3 43425 LSAVHXS 5 180d 3ON3TIVH2 WALSEN 9317113 Vd3H 431713 Adds WANS 1d 35055339 3015 2 ONICVOT WAS 831714 15 Vd3H 15 A311332 01 11182 3ADW3B 71149 3A1123108d 18 355 ONYE 59
32. IN BLOW MOTOR LO CLO INHIB CONTROL GND IIT CIT L1 SENSOR USB COMM TEST POINTS MOTOR ENABLE PWM SIGNAL DC VOLTAGE RPM FEEDBACK GROUND The cabinet is considered to be certifiable if the following airflow measurements are present a Downflow average 60 LFPM 5 LFPM 30 m s 025 m s b Inflow average 105 LFPM 5 LFPM 53 m s 025 m s using the direct inflow measurement method or constricted 3 inch 76mm high access opening measurement method Page 36 of 61 October 2014 7 5 3 1 Downflow Calibration Step 1 Place a velometer in the cabinet workzone on the horizontal plane 4 inches 102mm above the bottom edge of the viewing window Spot check several points on the recommended downflow velocity test grid in table 7 0 Step 2 If necessary enter active blower speed adjustment e Press and hold key then press hidden blower keys sequentially LED indicator above blower green key will blink fast Step 3 Press T or 4 keys to adjust blower speed e LED segments will indicate blower speed percentage and active blower speed adjustment o Right end red LED indicates active blower speed adjust The red LED will blink as soon as any adjustments are made and will continue to blink as the motor rpm settles Once the red LED stops blinking the motor will run steady state at the new percentage Note The red LED must be non blinking to save or exit o Green LED s indicate percentage o
33. IOSH and ASHP that the cabinet be exhausted to the outside In addition if this cabinet is used in microbiological application with minute quantities of volatile toxic chemicals and trace amounts of radionuclides per CDC NIH and NSF it is strongly recommended that the cabinet be exhausted to the outside NuAire offers a canopy type of exhaust transition which will capture the exhaust efflux from the cabinet Canopy Thimble or Air Gap Exhaust Transitions with and without integral fan Note The Exhaust System should be fitted with backdraft damper to prevent reversing of airflow the system NuAire only recommends a canopy or thimble transitions for exhausting applications See separate instruction sheets for a discussion of exhaust transitions and installation requirements Page 14 of 61 October 2014 5 2 Set Up Instructions Remove outer shipping protection carton or crating The cabinet is fastened to the base skid and it is usually the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling It can then be removed from the skid by removing the banding bolts and screws holding the cabinet to the skid It may be necessary to remove the control center in order to gain passage through a doorway It can easily be removed by following the instructions on drawing BCD 16301 5 2 1 5 2 2 5 2 3 5 2 4 0217 October 2014 It is recommended
34. ISRUPTIVE AIR CURRENTS FROM AIR VENTS The exhaust sensor for the Electronic Airflow Control System is located just above the exhaust HEPA filter and if disruptive air currents are present the exhaust sensor could be influenced by them and indicate disruptive readings on the front panel display If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening the potential exists for contaminated air to exit or enter the work zone area of the cabinet It depends on the severity of the air current REMEMBER A BIOLOGICAL SAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE Where space permits a clear 6 152mm area should be permitted on each side of the cabinet for maintenance purposes The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is inaccessible such as a conduit hardwired connection then an appropriate warning label should be applied near the cabinets on off switch to indicate the circuit breaker on the power distribution panel should be used A MINIMUM CLEARANCE OF 6 152MM IS REQUIRED FROM THE TOP OF THE CABINET TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX If this cabinet is used in a pharmacy application it is strongly recommended Per OSHA N
35. LFBSC s leave them in operation beyond the time of actual use Wipe Down The interior surfaces of the workspace should next be disinfected see Cleaning Procedures by wiping them thoroughly with 70 alcohol or similar non corrosive anti microbial agents USE OF CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL Materials and Equipment The apparatus and materials should next be placed into the cabinet Care must be exercised that no items be placed over the front intake grills Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet away from open face as possible Air Purge Additional purging of the workspace without user activity should be allowed for 2 3 minutes after materials and apparatus have been placed in it This will rid the area of all loose contamination that may have been introduced with the items Perform Work The work can now be performed The technician performing the work is encouraged to wear a long sleeved gown with knit cuffs and rubber gloves This will minimize the shedding of skin flora into the work area and concurrently protect the hands and arms from viable agent contamination At a minimum the hands and arms should be washed well with germicidal soap before and after wo
36. LabGard ES Energy Saver Class Il Type A2 Laminar Flow Biological Safety Cabinet Models NU 540 300 400 500 600 Bench Console Operation and Maintenance Manual October 2014 Revision 3 Series 1 LISTED Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 0 0217 Page 1 of 61 Congratulations You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available With proper care maintenance certification and laboratory procedure this cabinet will give you years of product and personnel protection from particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other reference and guideline materials are available through the following web sites www hc sc gc ca www cdc gov od ohs www absa org www cabs acsb ca www ebsaweb eu www inspection gc ca www who int www biosafety be www hse gov uk www nsf org www cetainternational org Wwww nuaire com 0 0217 2 61 October 2014 About this Operation and Maintenance Manual The information contained in this manual is intended to reflect our current production model along with the more frequently purchased options Any unique additions modifications or shop drawings are appended in the back flap of this manual along with
37. SVO SONSINZANOO 804 13 18 2 30 0317201 SI 1301803 TWH V 3NDZ 1 80220 ATWIIN3OI22V 9 571195 ATWILNYLSENS OL 349153 M eet eve m Tie IVON D33 AZ Page 16 of 61 0 0217 October 2014 5 3 0 0217 5 2 5 Plumbing Services Service ball valves with the type of service specified by the removable button on the handle are located in the work zone The service ball valves are not recommended for pressure over 75 p s i 5 2 BAR Reducing valves should be installed external to the cabinet if necessary Service ball valves should never be used for flammable gasses or oxygen service A special needle valve for oxygen service or certified valve is required and available upon request External connection is to 3 8 inch NPT coupling in the inner sidewalls Connection to plant utilities should be made with proper materials for the individual service and according to national and or local codes Observe all labels pertaining to the type of service and operating pressure 5 2 6 X Electrical Services The NU 540 series Biological Safety Cabinets may be hardwired optional or plugged into an outlet with protective earthing connection with the standard power cord The cabinet requires 115 VAC 60 Hz single phase current rating varies per
38. Surface Surface Cabinet Size Inches mm Height Fully Assembled 61 7 8 1572 61 7 8 1572 61 7 8 1572 61 7 8 1572 Height Minimum for Transport 59 1499 59 1499 59 1499 59 1499 Width 41 5 8 1057 53 5 8 1362 65 5 8 1669 77 5 8 1972 Depth with Armrest Removed 317 16 799 317 16 799 317 16 799 317 16 799 Work Access Opening Inches mm Standard Opening Height Optional 10 254 8 203 10 254 8 203 10 254 8 203 10 254 8 203 Standard Inflow Velocity 105 FPM 53 m s 105 FPM 53 m s 105 FPM 53 m s 105 FPM 53 m s Work Zone Inches mm Height 28 1 2 724 28 1 2 724 28 1 2 724 28 1 2 724 Width 34 3 8 873 46 3 8 1178 46 3 8 1178 70 3 8 1788 Depth measured at 10 inches 254mm 25 3 4 654 25 3 4 654 25 3 4 654 25 3 4 654 window height Standard is safety plate sliding glass 21 533 open 21 533 open 21 533 open 21 533 open Required Exhaust CFM CMH 10 254 8 203 opening 10 254 8 203 opening 10 254 8 203 opening 10 254 8 203 opening Standard Optional CMH CFM CMH CMH CMH Canopy Variable Flow Thimble NU 911 276 501 649 851 363 588 617 1000 451 676 766 1149 538 763 915 1297 255 450 383 765 295 520 502 884 365 590 621 1003 436 661 741 1124 Canopy Fixed Flow Thimble NU 907 320 544 269 457 426 724 359 610 531 902 445 756 634 1077 532 904 Plant Duct Static Pressure Eng Metric 0
39. TI Red LED indicator above blower will blink fast Select password Press 7 or 4 key to scroll through the code choices below No Password password password default password only password password password e Press hidden key to accept time and exit e Press Key at any time to abort and exit c Note If the required blower password option is selected in the blower airflow option menu see section 8 2 2 Then the No password choice above is not available and the default remains B H B 0217 Page 24 of 61 October 2014 6 2 Operating Guidelines The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow Biological Safety Cabinet LFBSC to control airborne contaminants of low to moderate risk as stated in Technical Report No FPS 56500000001 prepared by Dow Chemical U S A for the National Cancer Institute May 1 1972 Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in order to obtain a maximum potential for safety and protection The pre planning necessary to develop these protocols is based on several fundamental considerations each of which will cont
40. access opening 3 Along the edges of the entire perimeter of the work opening approximately 1 5 inches 38mm outside the cabinet with particular attention paid to corners and vertical edges 4 2inches 51mm from the sides up inside of the window at the side channel seals and along inside of the cabinet along the top of the work area or immediately below the wiper gasket The criteria used to evaluate the smoke patterns is the following 1 2 3 4 The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux No smoke shall escape from inside the cabinet No smoke refluxes out of the cabinet once drawn in nor does smoke billow over the worksurface or penetrate onto it No smoke shall escape from the cabinet 7 8 Site Installation Assessment Tests These tests are performed to verify the sash position airflow or pressure setpoint where an audible and or visual alarm will activate to signify unfavorable operating conditions within the biological safety cabinet and or the remote exhaust blower and canopy connection performance 7 8 1 7 8 2 7 8 3 0217 October 2014 Sash Alarm Step 1 With sash alarm switch enabled raise the sliding sash 1 2 5cm above the manufacturer s designated sash height for normal operation Verify that the audible visual alarm activates sounds Step 2 Return the sash to its normal operating height Airflow or Pressure Alarm when installed Step 1 Measure
41. aged Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the driver makes a notation on the consignee s copy of the freight bill Then an inspection must be made to verify the claim against the carrier This inspection is the basis of your filing the claim against the carrier 4 1 3 If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request inspection Without this inspection the transportation company may not accept a claim for loss or damage If the carrier will not perform the inspection an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request This along with other papers in the customer s possession will support the claim Page 13 of 61 October 2014 5 0 5 1 0 0217 Installation Instructions Location Within the laboratory pharmacy etc the ideal location of the biological safety cabinet is away from personnel traffic lanes air vents in or out doors and or any other source of disruptive air currents VENTALATION REGISTE BLOCKED WHEN FACING LABGARD CORRIDOI a AIRFLOW LABCARD LOCATION CASEWORK Suggested Cabinet Location in Laboratory THE EXHAUST FILTER AREA 15 ESPECIALLY SUSCEPTIBLE TO D
42. ain control board Check AC voltage coming out of main control board Check wiring to blower Check blower motor Check DC motor PWM signal on main control board Red alarm LED blinks Indicates a power interruption has occurred Press any key to clear Cabinet outlets won t turn on Blue LED above outlet key indicates the outlets should be on Check outlet fuse located on main control board Check voltage coming out of main control board Cabinet ultraviolet light won t turn on Yellow LED above UV light key indicates the UV lamp should be on Check sliding window position should be fully closed Check blower lights fuse on main control board Check voltage coming out of the main control board to ultraviolet light ballast Check ballast Blower or light fuse continues to blow after replacement N A Check for short on output of fuse Isolate output of fuse by disconnecting control center connectors light circuit AC or DC blower circuit etc to isolate the short UV LED blinks fast and red LED alarm Indicates that the UV light needs replacement Replace UV light and clear UV run time clock Blower green LED blinks fast and PresurFlow 3 green LED s indicators on Indicates that the Nite Care is activated preventing the usage of the cabinet N A PresurFlow left red LED indicator on and red LED alarm PresurFlow reading low flow pressure Check airflo
43. ained for maximum efficiency IF THE PROCEDURE DEMANDS USE OF A FLAME A BUNSEN BURNER WITH ON DEMAND IGNITION IS STRONGLY RECOMMENDED DO NOT USE CONSTANT FLAME GAS BURNERS During use the Bunsen burner should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect Page 15 of 61 00691 04 twee 81 141 340914 NI NADHS SY DESEAS 3Hl TWISNI ONY JADA NIYAT 51 1 02 1599 3AUH3N SPS OFS 0N SAWA 30 NOLLYTIVISNI 403 30 989310 53 08816 2 AT31VWIXUNddV H2N38 V NU 13 18 2 JHL 30914 WNISIHC SNITdnD3 8 391914 7 35 NOSIS ALY ONISN 401 H2N38 9 1935 ONY ONVHSSAD UWIS 2 V NO 13 18 2 3Hl 150 0 315 OL 310 71915 1 NAUHS SV 3015 1331 WalS 341235 Nn 319108 50 3 01 Q3HSINe n4 eve 3111907 00 JAVA Nivad 30 NOILVTIVISNI 204 0119104 ACOE 303 32 89313 NIV OL 315 3A WA WO84s 310 314 1 51534 11 HOIHA DL 37935 1 2 30 3sva 9NI3H 40 4 2 3 1123 ASN ONADAY CTIV WIS 5133 3SVE L3NI
44. and record the speed control operating voltage at the speed control test points using a voltmeter Step 2 Using the primary or secondary inflow test method lower the speed control voltage to reduce the inflow by 20 from the certified testing value Step 3 Verify that the alarm activates when the inflow is dropped to this point Step 4 Adjust alarm setpoint as necessary as instructed by the alarm manufacturer procedures Step 5 Return the speed control to its certified operating voltage as measured by the voltmeter Exhaust System Performance Canopy Exhaust Transitions Step 1 Introduce a visible medium source into the canopy air intake s while slowly reducing the exhaust volume until there is a loss of capture of the visible medium into the canopy air intake s The audible and visual canopy alarms shall respond within 15 seconds and the cabinet fan s will continue to operate Step 2 Return exhaust volume to original value NOTE Positive pressure ductwork conditions are strongly discouraged Positive pressure ductwork conditions should be reviewed and evaluated by a cognizant Safety Officer of Industrial Hygienist as part of their Laboratory Ventilation Management Program and Chemical Hygiene Plan Page 43 of 61 7 9 Cleanliness Classification Test for Pharmacy Application 0 0217 If this cabinet is going to used within pharmacy per 5 797 the cabinet must be tested to assure compliance to ISO 14644 1 1999 Cleanroo
45. arity of the air stream is not regained until a point is reached downstream approximately equal to three to six times the diameter of the object Within the parameters of this cone particles may be carried laterally by multidirectional eddy currents Transfer of viable materials and manipulations which may generate aerosols should not be performed above sterile or uninoculated materials Items should be localized on the work surface in clean and dirty groups Employ Aseptic Technique The operator must not assume an attitude of let the cabinet do it when performing procedures within a LFBSC Properly balanced and properly used cabinets will do an excellent job of controlling airborne contamination and containing viable agents but the cabinet will not eliminate contact transmission of contamination Normal laboratory contamination control procedures and basic aseptic techniques are necessary to obtain maximum benefit from the cabinet For example open bottle tube or flask mounts should be kept as parallel as possible to the downflow to minimize capture of chance particulates This precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environment should not be discontinued Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until surface decontaminated T
46. arms will produce an alarm tone for 30 seconds then ring back for 2 seconds of every 5 seconds Pressing the alarm silence key will silence the audible alarm for 15 minutes initially then will start the ring back function again The list below represents alarm types and their respective priority from the highest to lowest priority 1 New Firmware Loaded 2 Internal Board Failure 3 Poweron Reset 4 Airflow Pressure Alarm 5 Blower RPM Failure 6 Window High 7 Window Low 8 Replace UV Light Note The above messages are described in greater detail in section 8 Page 22 of 61 6 1 8 Operator Accessible Functions 6 1 8 1 Access and Navigation To access the operator accessible functions e Press and hold the key then enter the 3 key sequence for the desired function then release the key and follow each instruction set c Note Pressing the T Key at any time will abort and exit the process without saving any changes ES made Pressing the hidden key will accept all changes and exit 6 1 8 2 Auto Timer Duration Auto timer duration timers are countdown timers for the functions displayed once time is entered into a function The timer will begin to countdown upon the start of that function i e press UV light key to start timing the UV light The LED indicator above the function key will start to blink indicating the timer function If the LED indicator was full on no timer function is present Asthe timer exp
47. b Pressthe cleaning key on the front control panel to silence the audible alarm during the cleaning process c Apply appropriate disinfecting solution i e coverage plus Calgon Corp or similar disinfectant to cabinet surfaces Most surface disinfectants require a specific contact time depending upon the microbiological agents used within the cabinet CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER APPLICATION AND SAFETY PRECAUTIONS DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL CAUSE DAMAGE TO THE STAINLESS STEEL SURFACES IF LEFT ON FOR LONG PERIODS OF TIME d After the specified contact time wipe up excess disinfectant IF THE DISINFECTANT USED CONTAINS CHLORIDES OR HALOGENS RE WIPE ALL SURFACES WITH 7096 ALCOHOL OR SIMILAR NON CORROSIVE ANTI MICROBIAL AGENT TO PREVENT DAMAGE TO STAINLESS STEEL SURFACES Page 28 of 61 October 2014 6 6 Hazardous Drug Decontamination Procedures This procedure is intended to provide guidance following a spillage and or periodic maintenance testing or relocation of the cabinet Additional guidance can be provided by the CETA document CAG 005 2007 found at the CETA website www CETAinternational org 6 6 1 Preparation Prior to beginning decontamination activity personnel should wear proper personnel protection equipment PPE i e Tyvek isolation gown 2 pair of Nitrile gloves and a full faced HEPA filtered respirator All protective garments should be contained in 4 mil plastic
48. be turned on at any window height Note In addition to the Allow UV light anytime system function there is a double redundant UV window interlock relay To override the UV light window interlock relay the relay itself must also be shorted See electrical schematic for reference 0 1000811001 1008011001 Manual Blower Restart Normally when the blower is actively running and a power interruption occurs The blower will automatically come back on when power is restored If this function turned off the blower will not automatically come back after a power interruption but would require the user to press the blower key to restart the blower Nite Care Normally the Nite Care function is turned off If selected and turned on once the blower is actively running Upon closure the blower will continue to run at calibrated lower speed level to keep the workzone interior sterile The PresurFlow will indicate s blinking green LEDs along with green LED above blower key The fluorescent light will turn off blue LED above light key will blink if window closure turned it off and become inoperable however the UV light can be used Disable PresurFlow If this function is active the digital pressure sensor and alarm function are turned off Temporary Nite Care When this option parameter is turned on and the other requirements below are met the blower key when held for 5 seconds will toggle blower b
49. cabinet motor type and size for proper performance characteristics e Select Verify cabinet model and size Bold items represent default parameters o Press and hold E key then press blower blower blower keys sequentially LED indicates above both blower red and UV light keys will blink fast Review cabinet model size and change if desired as described below o Press T keys to scroll through the LED segment choices associated with model size Class 3 foot Class 4 foot Class 5 foot Class 6 foot Laminar 3 4 foot Laminar 5 6 foot o Press hidden key to accept model size and exit o Press J key at any time to abort and exit OM0217 Page 34 of 61 October 2014 e Select motor control function type o Press and hold key then press light light light keys sequentially LED indicators above both blower red and light keys will blink fast Review motor control function type and change it desired as described below o Press T or keys to scroll through the LED segment choices associated with motor control function type Auto DC ECM motor Fixed duty DC ECM motor Fixed duty EC Impeller motor o Press hidden key to accept motor control function type and exit o Pr
50. cabinet size reference Electrical Environmental Requirements It is recommended that power to the cabinet whether hardwired or plug connected be on its own branch circuit protected with a circuit breaker at the distribution panel near the cabinet 5 2 7 Final Assembly Remove the protective cardboard cover over the exhaust HEPA filter located under the protective screen if in place The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a soft cloth Harsh chemicals solvent type cleaners and abrasive cleaners should not be used Do not attempt to clean the HEPA filter media Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth Turn the cabinet on and let it operate for 60 minutes before using it as a LFBSC Testing Methods and Equipment After installation and prior to use NuAire recommends that the cabinet be tested or commissioned to factory standards As part of testing the certifier should go through the following initial checklist to assure all aspects of the LFBSC installation are complete and ready for testing e Review product installation Exhaust connection if present Damper valve installed correctly with label toward front if present LFBSC base stand level e Perform LFBSC certification At a minimum the following tests should be performed e HEPA filter leak test Downflow velocity test e Inflow velocity
51. d MOGNIM 5 SOLLSONSVIO W318A8 HOLIMS HOLIMS YOLINOW MOHYNS IHd WALSAS TOHINOO IOHLNOO AN3W LSnrav SNIDNVTIVB TWNHSLNI 3AL 03108d 8i SNIOvdS M 2 ez 91 mam exwam 2108 Hd 1 AN3Wisnrav 164 mes 8313431931 ISN 1SdHWHy 1wNOLLdO 602 8 QNvONVLS 18921 01 1529 3NOZWMOM 85 2 WHOM 155 188 11 OL 5 YE Alddns vd3H lasle x 224189 x TENVd 1NOH4 INNON HSfY14 15 Vd3H 2549 x x 1805102 HOLOW 91 181 Page 12 of 61 0 0217 October 2014 3 0 4 0 4 1 0 0217 Warranty NuAire Inc warrants Class 11 Type A2 w ECM Motor LabGard ES that it will repair F O B its factory or furnish without charge F O B its factory a similar part to replace any material including HEPA filters in its equipment within 60 months after the date of sale if proven to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned properly identified to the company at its factory charges prepaid Factory installed equipment or accessories are warranted only to the extent guaranteed by the
52. ess key at any time to abort and exit 0 0217 Page 35 of 61 October 2014 0 0217 7 5 3 Airflow Calibrations Failure to calibrate airflow to the specified requirements may result in unsafe conditions of performance i e product and or personnel protection noise and vibration The NU 540 airflow calibration consists of adjustments to balance the airflow within the cabinet THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS WITH A SUITABLE VELOMETER NuAire provides two adjustments to balance the airflow within the cabinet These are a PWM signal adjust via calibration parameter menu b exhaust filter choke The PWM signal or blower speed adjustment establishes the motor speed controls curve starting point of the programmed internal reference performance curve while the choke adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been NuAire s experience that the filters may not load evenly choke adjustments may be necessary for proper cabinet airflow balance and performance DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration The DC voltage may be measured using a digital voltmeter The two test points to measure DC ECM motor voltage are located on the DC motor connector on the main control board L NCCOM NO NC COMNO ALARM RELAY RELAY LE WIN WIN W
53. etween normal and Nite Care blower speed The Nite Care blower will time out based on the Auto Timer duration for Nite Care without a blower key press and the blower speed will revert back to normal Closing the window will automatically terminate In addition to turning this option on the following requirements must also be met e The Nite Care must be selected e Password must be selected e Window is at normal height and blower is running e Nite Care blower auto timer must be set for a minimum of 15 minutes Page 49 of 61 9 0 Remote Contacts The NU 540 has several contact closures for remote sensing of various functions 9 1 Fan Relay The fan relay contacts are normally open and closed contact closure outputs that are activated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an airflow alarm condition occurs Contact ratings are 250 VAC maximum at 2 Amps NCCOM NO NC COMNO BLOWER AC L OUT WIN WIN WIN BLOW 00 LO CLD INHIB leo EXH SENSOR 15 VDC TEST POINTS MOTOR ENABLE PWM SIGNAL DC VOLTAGE RPM FEEDBACK GROUND 9 3 15VDC Output The 15VDC 100mA output is generated if the blower is actively running 0217 Page 50 of 61 October 2014 10 0 Optional Equipment 10 1 Ultraviole
54. gree 2 0 Pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Chemical Exposure Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting USE OF CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL Chamber decontamination can be accomplished by paraformaldehyde vapor phased Hydrogen Peroxide or Ethylene Oxide without degradation of cabinet materials EMC Performance classified for light industrial Emissions EN61326 Immunity EN61326 Class A equipment is intended for use in an industrial environment In the documentation for the user a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Page 54 of 61 October 2014 12 0 Disposal and Recycle Cabinets that are no longer in use and are ready for disposal contain reusable materials ALL components with the exception of the HEPA filters may be disposed and or recycled after they are known to be properly disinfected Note Follow all local state and federal guidelines for disposal of HEPA filter solid waste BIOHAZARD Prior to any disassembly for di
55. h or chemical containment The LFBSC when used with proper technique is an effective primary engineering control in obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk hazard research and drug preparation or product operations as prescribed by the Center for Disease Control CDC Atlanta Georgia The NU 540 bench LFBSC meets the requirements of a Class since the cabinet conforms to the following requirements 1 Maintains a minimum inflow velocity of 100 LFPM 51mps through the work access opening 2 HasHEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum 3 Discharges a percentage of air to the outside atmosphere after HEPA filtration 4 Hasall biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure Cabinets used for work with minute quantities of non flammable or explosive volatile toxic chemicals and tracer amounts of radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust canopies 0 0217 5 61 October 2014 1 2 Safety structions These safety instructions describe the safety features of the LabGard ES Model NU 540 LFBSC The safety cabinet has been manufactured using the latest technological developments and has been thoroughly
56. he lamp is now directly exposed for replacement 4 The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting out the lamp 5 Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps cable or tubing during closure of the control center Available from Lab Safety Supply Janesville WI 53547 1368 or other laboratory industrial or hospital supply distributor 0217 October 2014 Page 31 of 61 7 3 HEPA Filter Motor Replacement The HEPA Filters do not need replacement under normal usage and barring an accident a puncture until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM 51 m s min This may permit the average downflow velocity to be as low as 55 LFPM 28 m s as long as no point falls below 20 of the average downflow velocity The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be biologically clean 7 3 1 Procedure Disconnect electrical power from the cabinet before attempting any maintenance action Step 1 Remove screws at each upper side of the control center and allow the control center to rotate down resting on the safety straps Second remove the front decorative panel which is held into position by 3 knurled nuts on the top edge and snap fit bullet catches on the bottom Step 2 Place sliding window into lowest
57. ided Dispose of spent HEPA filters properly Avoid direct contact to dirty side of the filters Place in sealed bag and label all waste containers cartons based on type of hazard Follow all Local State and Federal guidelines for disposal of HEPA filter solid waste b To remove the exhaust HEPA filter 1 Relaxthe exhaust filter seal loading mechanism by turning the four threaded bolts counterclockwise until one can see a definite release of the loading springs 2 Pullthe exhaust choke tray free and remove the filter It is not necessary to remove the tray although it is free to move forward several inches if necessary to free the HEPA filter 0217 Page 32 of 61 October 2014 0 0217 Step 4 Filter Installation Description Efficiency Airflow Rating Frame Type NU 540 300 NuAire Part Number Filter Size Filter Manufacturer NU 540 400 NuAire Part Number Filter Size Filter Manufacturer NU 540 500 NuAire Part Number Filter Size Filter Manufacturer NU 540 600 NuAire Part Number Filter Size Filter Manufacturer Supply HEPA Filter 99 99 0 3 Micron 100 55 05 per sq ft Metal A 980979 04 21 533mm x 32 813mm x 3 76mm Camfil Farr A 980979 01 21 533mm x 44 1118mm x 3 76mm Camfil Farr A 980979 02 21 533mm x 56 1422mm x 3 76mm Camfil Farr A 980979 03 21 533mm x 68 1727mm x 3 76mm Camfil Farr When installing
58. ing the hidden key will accept all changes and exit Pressing the key will abort the process and exit Sync Fan Relay with Active Blower Normally the fan relay will activate when the blower switch is pressed Blower can 1 1 i either be actively running or pending If the fan relay sync is active the blower must be actively running for the relay to change state Sync Accessary Outlet with Active Blower lll it Normally the accessary outlet is on all the time If the accessary outlet sync is active the blower must be actively running for the accessary outlet to turn on Sync Outlet Power with Active Blower lll Normally the outlet power is turned on via the outlet key If the outlet power sync is active the outlet power will turn on and off with the blower or may be turned on and off independently if the blower is active Sync Fluorescent Light with Active Blower Normally the fluorescent light is turned on via the fluorescent light key If the Lg fluorescent light sync is active the fluorescent light will turn on and off with the blower or may be turned on and off independently if the blower is active Sync 15 Volt DC output with active blower normally the 15 Volt DC output located on Il the control board is on when power is applied to the system If the 15 Volt DC output sync is active the blower must be actively running for the 15 Volt
59. ires the function will turn off e Select auto timer duration function o Outlets Press and hold key then press hidden outlet outlet keys sequentially LED indicator above outlet will blink fast Adjust desired time as described below o Lights Press and hold KE key then press hidden light light keys sequentially LED indicator above light will blink fast Adjust desired time as described below UV Light Press and Hold T key then press hidden UV light UV light keys sequentially LED indicator above UV light will blink fast Adjust desired time as described below o Nite Care Blower Press and hold KE key then press hidden blower blower keys sequentially LED indicator above Blower key will blink fast Adjust desired time as described below o Adjust countdown time Press or 4 keys to adjust time Time will change in 15 minute increments as shown on the PresurFlow LED segments e Press hidden key to accept time and exit Press Key at time to abort and exit 0217 Page 23 of 61 October 2014 6 1 8 3 Blower Password The blower on off password allows the cabinet user to place a 3 key sequence requirement to turn the blower on or off The 3 key sequence for the blower password will be a combination of the hidden and blower keys e Select blower password Press and hold key then press hidden blower hidden keys sequentially A
60. is known to be biologically clean or known to be chemically inert Surface disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protection Equipment Required specifications of NSF ANSI 49 Annex G EN12469 Annex J or applicable national state province Decontamination using hazardous gas vapor or mist must be performed in accordance with the or local regulations This procedure presents considerable risks and must be performed only by specially trained and authorized service personnel in accordance with applicable national safety regulations i e US OSHA NIOSH Germany TRGS 522 The decontaminate is generated either external or internal of the sealed cabinet The quantity of decontaminate should follow standard or manufacturer s recommendations based on cabinet volume The decontaminate process should follow standards or manufacturer s recommendations based on the decontaminate used All decontaminate materials are hazardous chemical liquid gas and vapor Flammable process and are required to be handled properly Follow all product and process documentation and labelling Page 30 of 61 October 2014 7 1 1 Manual 1 Disconnect power to the cabinet Remove screws at each upper side of the control center and allow the control center to rotate down resting on the safety straps Disconnect electrical connectors on left side Disconnect electrical
61. light on Outlet Keys The outlet key controls the ON OFF power to the outlets LED above indicates full blue for outlets on Red Alarm LED The red alarm LED will indicate any alarm condition and remain indicating until the alarm condition is cleared Audible Alarm Silence The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15 minutes After 15 minutes if the alarm condition still exists the audible alarm will again sound The audible alarm silence key also is used to exit all FlowGard user interaction menus Arrow Adjustment Keys The arrow adjustment keys allow user interaction for various functions Page 21 of 61 6 1 3 6 1 4 6 1 5 6 1 6 6 1 7 0217 October 2014 Aeromax Control System Power After the LabGard ES NU 540 is plugged into the appropriate facility line power the control system will power up The control panel will also indicate the power up status by blinking the red alarm LED Pressing any key will acknowledge the power up status and turn off the blinking red alarm LED If a power interruption occurs all control system functions calibrations and parameters will be maintained and continue upon restoration of power Just as the initial power up the red alarm LED will blink to indicate power up status Standby Mode When the BSC is not in use any of the function keys except the blower that initiates run mode may be turned on and off in standby mode
62. lines For each of the above evaluation criterion several work aids may be supplied to accommodate the user e Ergonomic chair A six way articulating seat and back control for personalized adjustment to assure proper user posture Be sure feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments e Forearm elbow support The cabinet is provided with a non metallic forearm support on the work access opening Periodic mini breaks during work practice should be taken resting forearm to avoid stress and fatigue Elbow rests that can provide support for particular work practices such as pipetting are optional Also available as an option closed cell foam disposable forearm pads to reduce pressure points and add comfort e Effective workzone layout Always prepare your work procedure to minimize reach to avoid neck and shoulder stress and fatigue Rotating tables used to maximum workzone and minimize reach are optional e Vision and sightline Always prepare your work procedure to eliminate glare and bright reflections on the window Keep your window clean and sightlines clear to your effective workzone Cleaning Procedures Cleaning the cabinet is an important function in terms of both containment and sterility Using the following procedure to effectively clean or surface disinfect the cabinet workzone surfaces a Raisethe sliding window to a full open position if desired
63. ms and Associated Controlled Environments Part 1 Classification of Air Cleanliness The cleanliness classification test is performed using a particle counter to measure particle counts within the cabinet workzone Turn on cabinet and let warm up for several minutes Turn on particle counter and flush out sample tubing line to remove latent particles Set the particle counter to measure 0 5 micron or larger particles at the appropriate measuring rate Operational Particle Count Test Position the particle counter isokinetic probe at a point 6 inches 152mm upstream of the aseptic manipulation area hand convergence point and mounted so as not to interfere with the operator s hand movement The pharmacy operator will simulate IV manipulation during the particle count test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Take 5 test points in 1 minute intervals on a grid horizontal plane as measured approximately 6 inches 152mm above the worksurface The grid location is designed as the workzone center point and each corner measured 6 inches 152mm from the inside perimeter Record the 5 particle count values for each of the test points over the 1 minute sample time All final count particle concentrations and calculated 95 upper confidence limit shall not exceed 3520 particles per
64. n available for Qualified Service Personnel Master Reset Resets all calibration cabinet size motor type function and option settings back to their default condition It is recommended to perform a master reset upon installation of replacement control board or if there seems to be intermittent functional abnormalities Perform the following sequence to accomplish a master reset 1 2 Turn off all functions i e blower lights etc Press light and UV light keys simultaneously and hold for 10 seconds until PresurFlow LED s all light up and go blank the release the keys Turn off cabinet either unplug cabinet or use power switch on left side within control panel then turn back on to reinitialize the Aeromax control board Just as in the initial power up the RED alarm LED will blink to indicate power up status Press key to clear the power on reset message Enter into service parameters and input configuration for calibration and option settings as required 8 0 Error Messages Troubleshooting Option Diagnostics and Airflow Sensor Performance Verification Audible alarms and error messages occur for a variety of reasons Whenever an alarm condition has been present for a period of at least 10 seconds the audible alarm error message will be presented and stay on until the error is cleared The audible alarm will be on for 30 seconds upon initial alarm condition then once every ten seconds When presented with an error message
65. n of scale 0 100 o Yellow LED s indicate minimum left maximum right blower speed has been achieved CS Note At any time during the process Press hidden key to accept and enter the blower speed calibration point If the blower speed calibration point was not successfully entered a half second audible alarm will occur The calibration process must then be repeated for successful entry of blower speed calibration point e Press key to abort and exit Step 4 Proceed to inflow calibration leaving in active blower speed adjustment 7 5 3 2 Inflow Calibration Step 1 Measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the exhaust filter choke located under the front decorative panel to achieve the correct average inflow velocity within the stated range of 105 5 LFPM 53 025 m s e Less than 100 LFPM 51 m s First open the choke plate or make sure it is open If this is insufficient then increase blower speed Greater than 110LFPM 56 m s First decrease blower speed to achieve the exhaust excess then close the choke plate to achieve the balance In this fashion the downflow should remain nearly constant i e what the reduced speed took away the choke plate restores ce Note The choke plate adjustment requires a standard blade screwdriver To adjust loosen the liquid tight fitting around the choke adjustment shaft While
66. new filters USE ONLY NUAIRE SPECFIED FILTERS FOR REPLACEMENT Exhaust HEPA Filter 99 99 0 3 Micron 250 fpm 23 05 w g sq ft Metal A 980957 17 20 508mm 14 366mm 6 152mm Camfil Farr A 9809957 18 20 508mm 22 559mm 6 152mm Camfil Farr A 9809957 19 20 508 x 762mm x 6 152mm Camfil Farr A 9809957 20 20 SO8mm x 36 914mm x 6 152mm Camfil Farr Toinstall the supply filter simply reverse the procedure outlines in Step above c Note Be sure to open the choke plate fully before inserting the filter into the tray This will assist in adjusting the airflow b Toinstall the exhaust filter apply a thin layer of silicone grease to the top and bottom gaskets of the filter and carefully insert into the exhaust choke tray Step 5 Motor Blower Assembly Removal Itis recommended that the motor blower to be removed as a single unit To remove October 2014 disconnect electrical connections to the motor remove the HEPEX pressure plenum and unbolt the motor blower assembly from the roof of the cabinet 4 places Always inspect the rubber isolation motor mounts and replace those that are cracked or visibly show stress b Replace the motor exactly as originally installed in the blower housing paying particular Position the filter frame within the outside walls of the exhaust opening on the top of the hood Tighten the spring loaded bolts 4 places
67. ntaminated and not accessible use both downflow and exhaust volume for determining challenge concentrations Use following area information below with average downflow velocity and spot check exhaust velocities as measured to determine volume CFM CMH Model Size Supply Area ft m Exhaust Area Model Size Supply Area Exhaust Area 300 6 33 588 1 61 150 500 10 74 998 3 15 293 400 8 53 792 2 38 221 600 12 95 1 203 3 92 364 Measured 4 inches above the bottom edge of the window Laskin Nozzle Concentration Formula Nozzles x 135 CFM x 100 ug L E Challenge Downflow CFM Exhaust CFM Concentration ug L Nozzles x 229 CMH x 100 ug L Challenge Downflow CMH Exhaust CFM Concentration ug L 0217 Page 42 of 61 October 2014 7 7 Airflow Smoke Pattern Test The airflow smoke pattern test is performed using a smoke source i e smoke tubes in and around the cabinet workzone and access opening to determine a visual representation of the cabinet s containment performance To perform the test the smoke source should be passed through the following areas smoke source shall be passed 1 From one end of the cabinet to the other along the center line of the work surface at a height of 4 inches 102mm above the top of the access opening 2 From one end of the cabinet to the other 1 inch 25mm just inside the view screen at a height 6 inches 152mm above the top of the
68. olume ft min m s Access Opening Inflow Velocity fpm mps Constricted 3 inch 76mm high access opening measurement Inches mm 8 396 12 792 17 188 21 584 25 980 30 375 102 213 325 437 548 660 771 8 264 12 528 16 792 21 056 25 320 29 584 33 848 38 112 42 375 102 210 318 426 535 643 751 860 968 1076 4 8 198 12 396 16 594 20 792 24 990 29 188 33 386 37 584 41 782 45 980 50 178 54 375 102 208 315 421 528 635 741 848 955 1061 1168 1274 1381 4 8 158 12 316 16 474 20 632 24 790 28 948 33 106 37 264 41 422 45 580 49 738 53 896 58 054 62 212 66 375 600 102 207 313 418 524 630 735 841 946 1052 1158 1263 1369 1475 1580 1686 Number of Readings Average Velocity of Constricted Area fpm mps Average Velocity fpm mps X Constricted Area Constricted CFM m s of Constricted Area Area Volume Constricted Area Volume 5 8 or 10 ft m Average Velocity fpm mps Access Window Area of 8 or 10 Access Window Area Average Velocity of 8 or 10 fpm mps X Correction Factor Average Inflow Velocity fpm mps Access Window Area d Acceptance Criteria 1 Access Opening Inflow Velocity 100 to 110 fpm 51 to 56 m s Areas Correc
69. original manufacturer and this warranty shall not apply to any portion of the equipment modified by the user Claims under this warranty should be directed to NuAire Inc setting forth in detail the nature of the defect the date of the initial installation and the serial and model number of the equipment This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse abuse accident shipping damage improper installation or service or damage by fire flood or acts of God If the serial number of this product is altered removed or defaced as to be illegible the Warranty shall be null and void in its entirety The warranty is for the sole benefit of the original purchaser and is not assignable or transferable Prior to returning any item for any reason contact NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned to you as refused shipment or collect freight Shipments NuAire takes every reasonable precaution to assure that your LabGard ES cabinet arrives without damage Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase However damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LabGard ES cabinet to be sure that if damage has occurred the proper claims and actions are taken immediately Dam
70. oximately 5000 hours under specific test conditions The control system monitors UV light usage hours and when the 5000 hour point is reached The UV light LED indicator will blink fast along with the red LED alarm indicating the UV light should be replaced To reset the timer perform the following e Press and hold key the press UV light UV light UV light key sequentially and the reset will occur removing the above alarm condition It is also possible to approximate the UV light usage by the following Press and hold key and UV light key together for three seconds PresurFlow LED s will then indicate usage approximately 450 hours per LED light from left to right Indicates approximately 450 hours used Indicates approximately 900 Hours used d Indicates approximately 3600 hours used Note Before testing with lamp off the light may be cleaned with a lint free cloth dampened with alcohol or ammonia and water 0217 Page 52 of 61 October 2014 References Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation e Mold Spores Penicillium roqueforti Penicillium expansum Penicillium digitatum Aspergillus glaucus Aspergillus flavus Aspergillus niger Rhizopus nigricans Mucor racemosus A Mucor racemosus B
71. position and remove front filter panel which is held into position by Phillip pan head screws Once the screws are removed the panel is held into position by smooth weld studs located on the top corner of the front filter panel Use the window stop brackets as handles to remove the panel c Note Screws used in lieu of acorn nuts and lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest window position will require the armrest to be removed If the cabinet has the power window option access the Power Window Test menu to adjust the power window into its lowest position with the armrest removed Access Calibration Service menu then Service menu then Power Window menu to Power Window Test Step 3 Filter Removal It is not always necessary to replace both the supply and exhaust filters at the same time If during the course of certifications the downflow always falls off while the exhaust increases the supply filter is loading faster than the exhaust filter and only the supply filter may need replacement The opposite might also happen depending upon many factors a remove the supply HEPA filter 1 Remove the plenum screws in front of the supply HEPA filter 2 the permanent plenum and hold up with wire strap 3 Carefully remove the supply filter Direct exposure should be avo
72. rable to enter the Service menu and turn on the blower Air currents in the room can sometimes contribute to exhaust entrainment on top of the cabinet This can lead to false leak readings In order to reduce the probability of false leak readings it is recommended to cover half of the exhaust HEPA filter while scanning the uncovered section of the exhaust HEPA filter Exhaust Filter The exhaust filter is typically more difficult to check since protective grills charcoal filters or exhaust transitions could cover the filter Access panels are usually provided and should be removed If an air gap exhaust transition is provided the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux All exhaust blowers fans should be turned off during the check NOTE To avoid the window high alarm during the filter integrity check it is desirable to enter into the Blower speed adjustment menu and turn the blower on To accomplish this perform the following Press and hold KE key then press ENT 4 keys sequentially releasing the key after the 3 key sequence LED segments will indicate blower speed percentage However no adjustment is needed do not adjust e filter integrity test is completed Press E key to abort and exit N m Note If the upstream challenge port is deemed co
73. raviolet Tubes 0217 October 2014 Page 53 of 61 Microwatt seconds per 2 200 000 a 40 000 b 22 000 c 6 600 440 000 3 400 d 11 0 11 1 11 2 11 3 11 4 11 5 11 6 11 7 0 0217 Electrical Environmental Requirements Electrical Supply Voltage Fluctuations Not to Exceed 10 NU 540 300 115 VAC 60 Hz 1 Phase 10 Amps NU 540 400 115 VAC 60 Hz 1 Phase 12 Amps NU 540 500 115 VAC 60 Hz 1 Phase 12 14 Amps NU 540 600 115 VAC 60 Hz 1Phase 12 14 Amps UL UL C Listed Optional 15A power cord Accessory outlet removed Operational Performance for indoor use only Environment Temperature Range 60 F 85 F 15 C 30 C Environment Humidity 20 60 Relative Humidity Environment Altitude 6562 Feet 2000M Light Exposure Standard Fluorescent Lighting 150 ft candles 1614 LUX maximum intensity Installation Category 2 0 Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply Pollution De
74. rays of discard pipettes must be covered before removal from the cabinet aluminum foil may substitute for fabricated covers If an accident occurs which spills or splatters suspensions of etiologic agent around the work area all surfaces and items in the cabinet must be surface decontaminated before being removed Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a conventional method of minimizing chance contamination However the efficiency of this operation is usually related to the removal of airborne contamination occurring while the item is uncovered If the manipulation is carried out in an environment free of airborne particulates then the need for the flaming operation is essentially removed This is one of the additional advantages of the LFBSC use of the gas burner is seldom necessary The gas burner flame in one of these cabinets not only contributes significantly to the heat build up it also disrupts the laminar air streams which must be maintained for maximum efficiency If the procedure demands use of a flame BUNSEN BURNER WITH ON DEMAND IGNITION IS RECOMMENDED DO NOT USE CONSTANT FLAME GAS BURNERS It should also be only used from the center of the work surface to the right rear where resulting air turbulence will have a minimal effect DO NOT USE GAS BURNER ON THE LEFT SIDE OF THE WORK SURFACE DUE TO ITS INFLUENCE ON THE ELECTRONIC AIRFLOW CONTROL SYSTEM If cabinet air is inadvertentl
75. ribute to optimum benefits from the equipment a b d e 6 2 1 NOTE 6 2 2 NOTE 6 2 3 0217 October 2014 Know your Safe Work Area Minimize penetration of air curtain Minimize room activity Utilize unidirectional airflow Employ aseptic techniques Know your Safe Working Area The LFBSC safe working area is basically the worktray or depressed area All work should be performed on or above the worktray The area on or above the front grill is non safe working area It is important to maintain an air gap on both sides of the worktray before fastening in place The work tray as being part of the cabinet system has been designed to load up to 100 lbs 45 4kg of work materials Any additional loading should be evaluated by appropriate safety personnel Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should also be planned to minimize the number of times an operator s hands and arms must enter and leave the air curtain at the open face The ideal situation is to have everything needed for the complete procedure placed in the hood before starting so that nothing need pass in or out through the air barrier at the face until the procedure is completed This is especially important in working with moderate risk agents Avoid lifting arms hands inside the cabinet above the
76. rk in the cabinet For the preparation of antineoplastic drugs the following procedures summarize those contained in OSHA Technical Manual TED 1 0 15A Section VI Chapter 2 Controlling Occupational Exposure to Hazardous Drugs The above document should be thoroughly studied and reviewed prior to drug preparation in the cabinet It may be found at this website http www osha gov dts osta a sterile plastic backed absorbent drape should placed on the work surface during mixing procedures The drape should be exchanged whenever significant spillage occurs or at the end of each production sequence b Vials should be vented with a filter needle to eliminate internal pressure or vacuum Before opening ampoules care should be taken to insure that no liquid remains in the tip of the ampoule A sterile gauze sponge should be wrapped around the neck of the ampoule while opening d Final drug measurement should be performed prior to removing the needle from the stopper of the vial e Anon splash collection vessel should be available in the LFBSC to discard excess drug solutions Page 27 of 61 6 4 6 5 0 0217 6 3 6 Terminal Purging and Wipe Down Following completion of work allow the cabinet to run for 2 3 minute period without personnel activity to purge the cabinet A surface disinfection of the interior surfaces see Cleaning Procedures section should be repeated after removal of all materials cultures apparatus etc
77. rm Setpoint e Press and hold the key for seconds The left red LED will blink and the green LED s indicate blower speed Press or 4 keys to adjust blower speed to the desired airflow velocity low alarm setpoint value end red LED indicates active low limit blower speed adjust The red LED will blink as soon as any adjustments are made and will continue to blink as the motor rpm settles Once the red LED stops blinking the motor will run steady state ate the new percentage If the low alarm setpoint value is not within an acceptable range the left end red LED will blink at a very fast rate Note The red LED must be non blinking to save or exit Note At any time during the process Press hidden key to accept low alarm setpoint value e Press E key to abort and exit 24 e exiting the blower will go back to actual airflows High Alarm Setpoint Press and hold the 7 key for 3 seconds The right red LED will blink and the green LED s indicate blower speed e Press T or 4 keys to adjust blower speed to the desired airflow velocity high alarm setpoint value e Red end Red LED indicates active high limit blower speed adjust The red LED will blink as soon as any adjustments are made and will continue to blink as the motor rpm settles Once the red LED stops blinking the motor will run steady state at the new percentage If the high alarm setpoint value is not within an acceptable range
78. s collected in the plenum drain aspirate it using IV tubing into an evacuated container Label the evacuated container for disposal as chemotherapy waste j Clean the plenums drain area and wipe dry k Ifthe cabinet requires maintenance and or replacement of the HEPA filters the operation should be halted at this point to allow trained personnel to complete replacement of the HEPA and or maintenance action required 6 6 3 Assembly Replace front grill if removed Replace the work tray and carefully tighten the thumbscrews Replace perforated metal diffuser screen over the underside of the supply HEPA filter Wipe down all exposed surfaces of the work area with 7096 isopropyl alcohol Prepare for aseptic operation Available from Lab Safety Supply Janesville WI 53547 1368 or other laboratory industrial or hospital supply distributors American Society of Hospital Pharmacists 2006 ASHP Guidelines on Handling Hazardous Drugs Am J Hosp Pharm 63 1172 1193 0217 Page 29 of 61 October 2014 7 0 7 1 0 0217 General Maintenance All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedures required including both certification and repair Decontamination No maintenance should be performed on the interior of the LabGard ES cabinet area behind access panels unless the cabinet has been microbiologically decontaminated
79. sposal the cabinet must be decontaminated 2 1 RECYCLE LEAD FREE Component Material Base Cabinet Stainless Steel Front Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Panel Painted Steel Control Center Painted Steel Supply Diffuser Aluminum Exhaust Filter Aluminum HEPA Filter Frames Painted Steel Hepex Bag PVC Blower Wheel and Housing Steel Motor Various Steel Printed Wiring Assembly Lead Free Electronic Wire PVC Coated Copper Ballasts Various Steel Electronic Armrest PVC Connectors Nylon Hardware Stainless Steel and Steel Note Material type can be verified with use of a magnet with stainless and aluminum being non magnetic 0217 Page 55 of 61 October 2014 999009 0263131 QNVIS3SVB A18W3SSV NOLLO3S 43 9 162 zit 02 44 6 gers zii 88 Lay zn Ob 8 s6 008 v 828 21 ve 8 MANOS GH XH LSS v E 2 X 91 8 8 LY14 8 8 H3HSVM 3201 1001 TVNH3LENI H3HSVM 1914 155 9 1 8 LAN NHOOV LSS 91 8 H3HSVM 20 1001 1 L108 155 005 X 02 511 ONIHVA JO 101108 L3NIBVO JO 3SV8 OLNI 2 83HSVM 13 13 LSS 8 H3HSVM HLOO L TVNH3 NI 2 1108 GH LSS 09 X 9 8 8 9 8 1 OS Z 4313 3
80. t Light 10 1 1 10 1 2 10 1 3 0217 October 2014 Ultraviolet light will injure your eyes Avoid direct viewing at all times Personnel should not be present when ultraviolet lamp is on Overview The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the air or on directly exposed surfaces Approximately 95 of the ultraviolet radiations from germicidal tubes are in the 253 7 nanometer region This is a region in the ultraviolet spectrum which is near the peak of germicidal effectiveness The exposure necessary to kill bacteria is the product of time and intensity High intensities for short period of time or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria disregarding the life cycle of bacteria The intensity of light falling on a given area is governed by the inverse law that is the killing intensity decreases as the distance increases from the tube The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per centimeter for a new tube falling on a horizontal plane defined by the bottom of the work surface The minimum requirement per paragraph 5 12 of NSF Standard 49 is 40 microwatts per square centimeter ref NSF Std 49 June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet
81. terior outlets and fan during the decontamination Set control center on its side resting on the left side of cabinet Then plug in the 16 pin connector cable from the cabinet to the control center Reconnect power to the cabinet The outlets and blower can then be activated Seal front and top openings using plastic and tape BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS Perform decontamination procedure Please consult with NuAire Inc about any unique contamination problems Normally no preventive maintenance is required on the interior of the cabinet i e the area behind the access panel containing the HEPA filters and motor blower assembly All required adjustments in order to maintain proper cabinet airflows are external to the cabinet interior The motor is lubricated for life and is thermally protected with automatic reset 7 2 Fluorescent LED Lamp Replacement The two T8 fluorescent bulbs or optional LED lamps are cool white and placed external to the cabinet to aid maintenance and minimize heat build up within the cabinet The life rating of the bulb is 9 000 for fluorescent and 50 000 hours for LED based on three hour burning cycles To replace a lamp it is necessary to remove the lamp assembly 1 Switch cabinet light switch off 2 Remove the screws at each upper side of the control center and allow the control center to rotate down resting on the safety straps gw T
82. tested before delivery However the cabinet may present potential hazards if it is not installed and used as instructed for its intended purpose or is used outside of operating parameters Therefore the following procedures must always be observed The safety cabinet must be operated only by trained and authorized personnel For any operation of this cabinet the operator must prepare clear and concise written instructions for operating and cleaning utilizing applicable safety data sheets plant hygiene guidelines and technical regulations in particular o which decontamination measures are to be applied for the cabinet and accessories o which protective measures apply while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operating instructions close to the cabinet so that safety instructions and important information are always accessible Should you encounter problems that are not detailed adequately in the operating instructions please contact your NuAire Representative of NuAire technical Services 1 3 Explanation of Symbols 4 Os 0217 October 2014 Safety alert symbol indicates a potentially hazardous situation which if not avoided could result in death of serious injury Safety alert symbol indicates a potentially hazardous situation which if not avoided ma
83. that no less than two people are present using a lifting system for placement of the cabinet onto the base stand It is not recommended to manually lift the cabinet onto the base stand Base Stand Assembly The base stand is typically shipped knocked down in a separate carton and is assembled per drawing BCD 16385 Once assembled place the stand in approximate position ready for cabinet placement onto the base stand Now lift the cabinet on top of the base stand and bolt the base stand to the cabinet using two 3 8 16 x 3 4 bolts and washers provided for the front base stand tabs and two 1 4 acorn nuts for the rear weld studs Place the cabinet in the desired location Leveling Using a level placed on the work tray first adjust the leg levelers end to end and then front to back The NSF approved leg levelers provide a 3 4 20mm adjustment Bench Installation BCD 16300 Place the cabinet on the bench with approximately a 2 51mm overhang clearance for installation of the drain valve Place the cabinet in desired location and using RTV caulk seal all around the base of the cabinet and the bench This provides a tight seal to prevent bench spills from migrating under the cabinet If the drain valve is desired NOTE CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS OF DRAIN VALVE INSTALLATION remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate
84. the right end red LED will blink at a very fast rate Note The red LED must be non blinking to save or exit Note At any time during the process e Press hidden key to accept low alarm setpoint value e Press E key to abort and exit e Upon exiting the blower will go back to actual airflows Note Specific use alarm setpoints will be lost upon entry of new nominal calibration value It is necessary to re enter specific use high low alarm setpoints every time a new nominal calibration is entered 0217 Page 38 of 61 October 2014 7 5 3 4 Nite Care Calibration The Nite Care mode is defaulted to operate the blower at approximately 600 rpm or a 14 duty cycle However if desired the Nite Care blower speed can be adjusted higher or lower by performing the following Press and hold key then press hidden blower keys sequentially LED indicator above blower green key will blink fast Press T or 4 keys to adjust blower speed LED segments will indicate Nite Care blower speed percentage active blower speed adjustment o Right end red LED indicates active blower speed adjust The red LED will blink as soon as any adjustments are made and will continue to blink as the motor rpm settles Once the red LED stops blinking the motor will run steady state at the new percentage Note The red LED must be non blinking to save or exit o Green LED s indicate percentage on of scale 0 100 o Yellow LED s indicate minimum
85. tion Factors for Calculations Cabinet 3 76mm 8 203mm 10 254mm Correction Factor for Correction Factor for Size Constricted Window Window Access Window Access 8 203mm 10 254mm Access Area Opening Opening Window Window 72 1 91 2 39 880 067 177 222 me vB 97 2 58 3 22 090 240 299 gi 20 1 22 3 24 4 05 300 113 301 376 102 192 1 47 3 91 4 89 S 137 363 454 102 1402 0 0217 Page 41 of 61 October 2014 7 6 HEPA Filter Leak Test In order to check filter and filter seal integrity the HEPA filter media and seals must be directly accessible by the measuring instrument The challenge material i e PAO should be supplied in the rear center of the workzone over the intake slots The upstream challenge port being common for both filters is located under the work surface with a red cap 7 6 1 7 6 2 Supply Filter The diffuser plate placed below the HEPA to protect the filter during normal usage may be removed as follows The diffuser is secured to the cabinet shell by 1 4 20 acorn nuts located immediately behind the front viewing window After removing the fasteners drop the front of the diffuser plate several inches and pull forward gently Note that the diffuser is purposely a tight fit it is held to the back wall of the cabinet interior by a light push fit with projecting studs To avoid the window high alarm during the filter integrity check it is desi
86. uorescent Lamp Bulb Replacement Ee HEPA Filter Motor Replacement et abre etus E EINER Sliding Window Replacement and Manual Adjustment JV Des e E RETE Airflow Control System Setup and Calibration yo HEPA Filter Leak test Jud bd t Airflow Smoke Pattern Test J B vise Site Installation Assessment Test a tiet Cleanliness Classification Test for Pharmacy Application Main Control Board Description and Replacement Section wrest Error Messages Troubleshooting Option Diagnostics Section No 9 Remote Contacts Section No 10 Optional Equipment Re Pd Ultraviolet Light Section 11 Electrical Environmental Requirements Section 12 Disposal and Recycle Replacement Parts Manual Drawings 0 165172 NU 540 Airflow Schematic BCD 16513 cased NU 540 300 Specification Drawing 16514 NU 540 400 Specification Drawing BCD 16515 NU 540 500 Specification Drawing BCD 16516 NU 540 600 Specification Drawing BCD 16300 Drain Valve Installation BCD 16521 Front Label Assembly Drawings gt 1663 Base Stand Assembly BCD 16301 Control Center and Front Decorative Panel Assembly BCD 16376
87. utlets via solid state switch Disable audible alarm switch with ring back function Control blower DC ECM motor with solid state DC Motor Controller that provides automatic compensation constant volume control for both filter loading and line voltage variances e Monitor and display airflow system performance via PresurFlow monitor The LabGard ES NU 540 offers the latest digital microprocessor design technology for improved cabinet performance and safety The Aeromax control system integrates a digital pressure sensor PresurFlow to monitor the cabinet s airflow performance The Aeromax control system also integrates a DC motor controller that provides automatic compensation for both filter loading and line voltage variances There is additional on off control of blower fluorescent light ultraviolet light optional and outlets Lastly the Aeromax control system monitors the sliding window position with micro switches diagram see figure 1 DIGITAL INPUT MICROPROCESSOR All the above functions are shown a system block WATCH DOG AC OUIEMTS PRESSURE SENSOR IN BLOWER _ UV LIGHT FLUORESCENT LIGHT OUTLET 1 ACC OUTLET SWITCH INPUTS WINDOW CLOSED MAIN MICROPROCESSOR WINDOW HIGH 2 4 DC OUTPUTS 15 1 CONTACT CLOSURE INPUTS RELAY CONTACT CLOSURE T KEYBOARD WILED S OUTPUTS BLOWER AND 4
88. w values Check blower function Recalibrate PresurFlow system PresurFlow right red LED indicator on and red LED alarm PresurFlow reading high flow pressure Check airflow values Recalibrate PresurFlow system All PresurFlow LED s blink Message acknowledges new firmware was loaded into microprocessor N A Blower red LED blinks and red LED alarm Indicates that the motor rpm signal has been interrupted Check connectors and wires from main control board to the motor Replace motor if required 0217 October 2014 Page 47 of 61 8 2 Option Parameters The option parameter menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the menu as described below 8 2 1 Sync Function with Active Blower To access the option parameter menu perform the following e Press and hold key then press hidden Blower Fluorescent keys sequentially Red LED indicator above the blower key will blink fast The PresurFlow blinking green LED segments will indicate seven optional parameters as shown and described below The UV Light key move lefts and outlet key move right allows selection of the option parameter desired Once the desired option parameter is indicated press T or 4 key to turn on or off A slow blinking green LED indicator means off and a fast blinking green LED indicator means on Multiple option changes can be selected e Press
89. y result in minor or moderate injury CAUTION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property CAUTION Potential electrical hazard only qualified person to access NOTE Used for important information Biohazard Flammable Hazard Ground Earth Lead Free Hazardous Gases Personal Protection Equipment Required Chemical Hazard Page 6 of 61 e JR WIN 1591 0329 1 133HS OHO 102791721 JIIVW343HOS 711149 LINDJA preset 31950 353 111849 293 206 11145 1 woos 319 77 WaDSINA 2192 N3dVd 77150 TVANVd 2 CONISOWD 11042 1200 aly 0 0 1 9 101715 3 111504 7 3 11993 0 _ WINS Id 9350 35 Vd3H WANIYA 2 113 8 11 3 34119509349 Vd3H 53431714 Vd3H 15 ANY 5 01 553009 404 419 3331313 ONY A3NVd 831113 IND34 Ily 3XNLXIW aIv 131911241934 1331999 LHOIL 3anssadd 51 1791721 NOllondodd 01 03593134 68121 V 21440 3190 01141582530 023 Page 7 of 61 0 0217 October 2014 2 0 Models and Features The model NU 540 LabGard ES Class 11 Type A2 Laminar
90. y turned off the flame could damage the HEPA filters Page 26 of 61 6 3 Operating Sequence 6 3 1 NOTE 6 3 2 6 3 3 6 3 4 6 3 5 0217 October 2014 Start Up Turn on cabinet blower and lights and check air intake and exhaust portals of the cabinet to make sure they are unobstructed The electronic airflow control system will automatically control airflows to specified setpoints However upon filter loading the cabinet may be required to be re balanced or filters replaced Only a qualified maintenance technician should perform cabinet balancing and filter replacement Some cabinets are equipped with ultraviolet UV lights Good procedure includes the decontamination or wipe down of cabinet surfaces with chemical disinfectant before work commences This practice eliminates the need for UV lights whose primary utility in this application is inactivation of surface contamination since the filters effectively remove all airborne contaminants UV lights therefore are not recommended in the LFBSC Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the cabinet If the filtered air exhausted from the cabinet is discharged into the room as in some installations an additional advantage is obtained from purification filtration of the room air circulated through the equipment Because of this characteristic contributing to the quality of the laboratory environment some owners of

O&M Manual for NuAire NU-540 BioSafety Cabinet

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O&amp;M Manual for NuAire NU-540 BioSafety Cabinet

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O&M Manual for NuAire NU-540 BioSafety Cabinet