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Spark User Guide

1. Message type 0x00110010 Regarding user specified Byte 16 Byte 17 Byte 18 Byte 19 Byte 20 Byte 21 Byte 22 Byte 23 0x00 0x04 Reseed Checksum Immediate type length Byte 24 Byte 25 Byte 26 Byte 27 Byte 28 ei Byte 39 X E X X 0 0 0 LSB MSB Integration time immediate data Unused Byte Byte Byte Byte 40 41 42 43 0x14 0 0 0 Bytes remaining 911 00000 000 02 201504 49 Fo Ocean 7 Firmware and Communications Age Opt ICs Optional Byte 44 Byte 59 Byte 60 Byte 61 Byte 62 Byte 63 Payload Not used for Checksum OxC5 0xC4 0xC3 0xC2 this command Footer The following is an example of how the Get And Send Corrected Spectrum Immediately message type 0x001 010 00 can be constructed based on the information provided in this data sheet Header Byte 0 Byte 1 Byte 2 Byte 3 Byte4 Byte5 Byte 6 Byte 7 0xC1 OxCO 0x00 0x10 0x00 0x00 0x00 0x00 Start bytes Protocol version Flags Error number Byte 8 Byte9 Byte 10 Byte 11 Byte 12 Byte 13 Byte 14 Byte 15 0x00 0x10 0x10 0x00 X X X X Message type 0x00101000 Regarding user specified Byte 16 Byte 17 Byte 18 Byte 19 Byte 20 Byte 21 Byte 22 Byte 23 0x00 0x00 R
2. Spark Spectral Sensor User Manual For Products Spark VIS Spark OEM VIS Spark DET VIS Document 91 1 00000 000 02 201504 A HALMA COMPANY AMERICAS amp WORLD HEADQUARTERS Ocean Optics Inc Phone 1 727 733 2447 Fax 1 727 733 3962 Ra ace USA Sales info oceanoptics com Orders orders oceanoptics com Manufacturing amp Logistics Support techsupport oceanoptics com 4301 Metric Dr Winter Park FL 32792 USA EUROPE MIDDLE EAST amp AFRICA Phone 31 26 319 0500 Sales amp Support Fax 31 26 319 0505 Geograaf 24 Email info oceanoptics eu 6921 EW Duiven The Netherlands Germany 49 711 341696 0 seas UK 44 1865 811118 Manufacturing amp Logistics PN Maybachstrasse 11 France 33 442 386 588 73760 Ostfildern Germany ASIA Phone 86 21 6295 6600 Ocean Optics Asia Fax 86 21 6295 6708 666 Gubei Road Email asiasales oceanoptics com Kirin Tower Suite 601B Changning District Japan amp Korea 82 10 8514 3797 Shanghai PRC 200336 www oceanoptics com Copyright 2015 Ocean Optics Inc All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted by any means electronic mechanical photocopying recording or otherwise without written permission from Ocean Optics Inc Trademarks All products and services herein are the trademarks service marks registered trademarks or registered service marks of their respective owners Limit of L
3. __ ud Optical Fiber CUV UV Cuvette Holder Optical Fiber Spark VIS Spark ACC BASE Spark ACC SMA Computer Micro USB Typical Absorbance Set Up Common Absorbance Applications e Quantification of proteins in life science samples e Environmental monitoring of water impurities e Analysis of chemical composition of mixtures 10 911 00000 000 02 201504 Koco e Optics 2 Installation and Set up Reflectance amp Transmission Reflectance spectroscopy compares the relative level of light reflected off a sample compared with a reference given as a percentage of the reference spectrum at each wavelength A reflectance standard is used to set the reference level of 100 Transmission is similar but compares the light transmitted through a sample relative to a reference rather than reflected off it Typically Reflectance uses a fiber optic probe attached to a light source and a spectrometer but measurements can be done easily with the Spark both in free space or with the SMA Adaptor accessory Transmission setups are usually the same as Absorbance setups Reflectance bluLoop Standard Light Source Tablet PC Reflection Probe Spark VIS Reflection Probe Spark ACC BASE with Holder Spark ACC SMA Micro USB A Reflectance Set Up with Probe Reflectance Standard and Probe Holder Common Reflectance Applications e Diffuse and Specular Color Measurements of surfaces e Process control for
4. Includes the payload if any plus the checksum and Remaining footer Payload length must be computed as this field OxFFFFFFFF minus the checksum and footer length Spark may reject any message too long for it to process internally and return a NACK The header can be represented as a C struct as follows assuming that the int type is 32 bits long struct ooi binary protocol header un un un un un un un un un signed ch signed sh signed sh signed sh signed in signed in signed ch signed ch signed ch 911 00000 000 02 201504 ar start bytes 2 ort protocol version ort flags ort errno t message type t regarding ar reserved 6 ar checksum type OxCl OxCcO 0x1000 ar immediate data length 47 ay t cean 7 Firmware and Communications Opt Ka unsigned char immediate data 16 unsigned int bytes remaining te Payload After the standard header a payload may be provided The payload contains data required by the given message type The format of the data within the payload depends on the message type Note that a payload is not required if operands will fit in the Immediate Data field of the header The length of the payload must be computed from the Bytes Remaining field in the header given that the checksum and footer are of a constant length Payload length Bytes remaining 20 The Spark may populate the Immediate Data fi
5. communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense dd WARNING The authority to operate this equipment is conditioned by the requirement that no modifications will be made to the equipment unless the changes or modifications are expressly approved by the manufacturer 911 00000 000 02 201504 vii an t cecan Optics About This Manual viii 911 00000 000 02 201504 Chapter 1 Introduction Product Introduction The Spark spectral sensor is the first Ocean Optics spectral device to make use of a solid state optical sensor in place of the diffraction gratings normally seen in our spectrometer product range This change has enabled Ocean Optics to reduce the footprint of a miniature spectral device down to the size of an electronic component and to do so for a competitive and accessible cost Spark comprises a family of spectral sensor products and is available in three formats a core spectral sensor product and two embeddable OEM friendly versions with the level of integration up to the customer The Spark DET the smallest version is at the heart of the plug and play core device both shown below and weighing less than 1g the Spark DET is the smallest spectral device on the market A spectral sensor is a new class of device that focuses on size manufacturability and value bri
6. 15 Boxcar smoothing will apply to every pixel even if there are not a balanced number of neighbors on both sides 0x001 801 00 Get wavelength N A Unsigned Request has no input coeticient byte Reply has 1 byte payload with number count es of coefficients 0x001 801 01 Get wavelength Unsigned IEEE Request has 1 byte input data for coefficient byte single coefficient index starting with precision wavelength intercept at index 0 Reply has 4 byte float LSB first 0x001 801 11 Set wavelength Unsigned N A Input is the order of the coefficient to set coefficient byte IEEE indexing starts with wavelength single intercept at index 0 followed by an precision IEEE single precision float No reply 0x001 811 00 Getnonlinearity N A Unsigned Reply has 1 byte output data with coefficient byte number of coefficients count 0x001 811 01 Get nonlinearity Unsigned IEEE single Request has 1 byte input for coefficient coefficient byte precision index to retrieve Reply has 4 byte float LSB first 0x001 811 11 Set nonlinearity Unsigned N A Input is the order of the coefficient to coefficient byte IEEE set followed by an IEEE single single precision float precision No reply 56 911 00000 000 02 201504 ROEM 7 Firmware and Communications Message Type Purpose Input Data Output Data Notes 0x001 820 01 Get irradiance N A Up to 4096 Request has no payload calipran
7. 3 to 1 across range Corrected linearity lt 0 5 from 15 95 full scale 2500 14000 counts net Wavelength drift lt 0 006 nm C Electronic A D resolution 14 bit N A Power consumption 250 mA O 5 30 mA O 5 VDC VDC Connector micro B USB 12 pin 0 5mm 14 pin 7 x 2 power and pitch ribbon connector signal cable Detector Detector Panavision ELIS1024 linear silicon CMOS array 911 00000 000 02 201504 29 F Ocean 5 Technical Specifications Ver Ort Ics Specification Spark VIS Spark OEM VIS Spark DET VIS Integration time 10 ps to 10s Pixels 1024 Pixel size 7 8 x 125 um Pixel well depth 800 ke Sensitivity of Detector 6 74 V lux s at 555 nm Quantum efficiency 60 across spectrum peak at 675 nm Physical Operating temperature 10 to 60 C Storage temperature 30 to 70 C Relative humidity 0 to 85 Aperture size 8x 1 mm approx Dimensions L x W x H 53 3 x 36 4 x 38 4 x 22 6 x 18 42 x 9 65 x 19 9 mm 10 2 mm 9 53 mm Weight 159 3 89 lt 1g Compliance ac2 Electrical CE FCC CISPR 11 2010 EMC 2004 108 EC and EN 61326 1 2013 Material RoHS Manufacturing ISO 9001 1 Dynamic range for a single acquisition is a measure of the ratio of full signal to noise 2 Dynamic range of the system is the range of the detectable light level and can be thought of as the maximum detectable light level at the minimum integration time divided by the minimum
8. 911 00000 000 02 201504 59 q Cegn Optics A Q 7 Firmware and Communications 60 911 00000 000 02 201504 Chapter 8 Spark OEM VIS and Spark DET VIS Advanced Use This chapter is for those users who intend to integrate the Spark OEM VIS or Spark DET VIS into their own process or product Please read the previous chapters for instructions on the installation of your Spark VIS spectral sensor and general information about the device To use these formats of the product the user must supply driver electronics and software The Spark OEM or Spark DET units must then be connected to these electronics via either a ribbon cable mating socket or a pin output mating socket The outputs of these two formats of the Spark are both raw analogue signals The Spark OEM format has housing that may be attached to a surface via M2 5 screws It may also be connected to the Spark Base accessory to interface to all other accessories The Spark DET format is simply an electronic component so must be integrated onto a electronic board Housing for this part must be provided by the user Details for the ELIS1024 detector and the Murata thermistor components in both devices and for the two device outputs are given below Advanced Product Details Specification Spark ELIS1024 detector Supply current 20 mA Supply voltage 5 0 V Output voltage at saturation 4 8 V Output voltage at dark 2 1V Conversion gain 3
9. Mode On Demand Intensity counts Schematic Window Q GI i i i i MA 1 NM 460 500 550 iew Spark VIS A Wavelength nm Spark VIS e _Spark VIS Wavelength emt 432 806 g Spark VIS 2696 33 7 Saved Data 6 OceanView Main Screen 1 Acquisition Group Window Set acquisition parameters such as integration time Controls the spectral sensor acquisition 2 Schematic View Schematic view graphically displays the flow of information from the spectral sensor to the view Use nodes to mathematically modify the data to create processed measurements Extremely flexible and incredibly powerful 3 View Display Display your data view save and display controls other features such as peakfinder and quick dark amp reference 18 911 00000 000 02 201504 Koco e Optics 2 Installation and Setup 4 Global Controls Control all spectral sensors synchronously save projects start a new application wizard 5 Saved Data Displays data saved in the active save file path Preview data store notes and load overlays directly to the active view Connect the Spark in OceanView The Spark should automatically appear when you start OceanView and should start acquiring data with default acquisition parameters If you do not see a signal or the Spark icon on the schematic you may need to rescan for devices gt Procedure To rescan for attached de
10. OEM product Detector high volume only Plug and play via micro USB Requires driver and Requires embedding into Accessories available communication electronics custom electronics and housing Ribbon cable connector with Contact our Sales Team to raw analogue output discuss integration Accessories available opportunities Education OEM custom solutions High volume integration R amp D Medical devices Handheld devices Application testing Process monitoring Embedded systems 4 911 00000 000 02 201504 Chapter 2 Installation and Setup What s In the Box a Spark spectral sensor The Spark VIS and Spark OEM VIS products can be ordered individually with the items specified below The Spark DET VIS product may only be ordered in volume for OEM applications it would therefore not be shipped with any components other than the Packing List below a Packing List The packing list is inside a plastic bag attached to the outside of the shipment box the invoice arrives separately The packing list includes the shipping and billing addresses as well as any items on back order a Micro USB Cable for Spark VIS format only Use this cable CBL MICROTOA USB to connect your spectral sensor to a computer running on a Windows Mac or Linux operating system USB Type A to Micro B a Calibration Data Sheet for Spark VIS and Spark OEM VIS only Each Spark VIS and Spark OEM VIS spectral sensor is shipped with a Wavelength Calibratio
11. and the non linearity correction compensates for this Nonlinearity correction uses either a 4th order or a 7th order polynomial a 4th order polynomial can be thought of as a 7th with C5 C6 and C7 coefficients set to zero Many Ocean Optics devices including the Spark VIS format store the correction coefficients in the device the Spark OEM VIS and Spark DET VIS formats will have these coefficients stored on the customer s electronics The correction for nonlinearity is performed as follows Where P Pixel number starting at 0 Sp Scope mode intensity uncorrected counts of a sample at pixel p Dp Scope mode intensity uncorrected counts of pixel p for a stored dark spectrum c0 c1 C7 Non linearity correction coefficients 7th order shown here stop at c4 for 4th order Fp correction factor for pixel p Rp Corrected scope mode intensity of sample at pixel p x Temporary variable representing the sample minus baseline For every pixel P in the spectrum x Sp Dp Fp cO c1x C2x2 C3X3 C4X4 C5X5 COX6 C X7 Rp x Fp 911 00000 000 02 201504 37 Fo Ocean The resulting spectrum A is the nonlinearity corrected spectrum Typically the dark spectrum is added back in thus the end result is Rp Dp after doing all such corrections so that further spectral math can subtract the dark again without resulting in a negative baseline The process for computing these coefficients is somewhat involved Oc
12. detectable light level at the maximum integration time Tested with a broadband illumination source Contact info oceanoptics com to obtain copies of certifications 30 911 00000 000 02 201504 esse 5 Technical Specifications Mechanical Diagrams Note Dimensions are given in inches and mm in brackets MICRO UsB 1 387 35 24 2 097 53 27 Spark VIS Outer Dimensions Spark OEM VIS Outer Dimensions 911 00000 000 02 201504 31 Q Ocean S Technical Speciteations O O O SSS U UO O Eos 3 65 144 Spark DET VIS Detector Dimensions in inches 32 911 00000 000 02 201504 Chapter 6 Calibration Overview This chapter provides information for performing your own wavelength and irradiance calibrations An EEPROM flash memory chip in each Spark VIS the user must provide this for other two formats contains wavelength calibration coefficients linearity coefficients and a serial number unique to each individual device A wavelength calibration sheet is provided with Spark VIS and Spark OEM VIS products this contains the wavelength calibration coefficients unique to each device The operating software application reads these values directly from the device enabling the ability to hot swap devices between computers without entering the coefficients manually on each computer The Spark DET VIS format will need wavelength calibrating by the end user as the calib
13. ee ee ne es 21 Saved Data Panel sis codeine da eee ie ene eee 21 Projects and Methods wiitine isso o ENEE 22 Spectroscopy Application Wizards cccccccscceesceceeeeeceeeeeeaaeeeeeeeceaeeesaaeeeeaaeseeeeescaeeesaaeeseneeesaees 23 Dark and Reference Measurements ssssssssesssrresssrnesrsnnestennestnnnnntennennnnnnntennennnnnnntenneennnnnneenne 23 Schemat EE 24 Chapter 4 Troubleshooting 2 ccccceceeeeeeeeeeneneeeeeeeeeeeeeeeeeeeneneeeeees 25 Chapter 5 Technical Specifications s ccseeeeeeeeeeeeeeeeeeeeeeeees 29 Specifications eet 29 Mechanical Diagrams EE 31 Chapter 6 Calibration x sccsscinspentcentetanceissenctdncaceninnsacansnanataacssigeesadsancanae 33 EE 33 CEET e ere e EE 33 About Wavelength Calibration err earaaaaaaa are aaar aa aaaa ra nanananaaaa 33 Wavelength Calibrating the Spectral Sensor rear aereas 34 Preparing for Calibration EE 34 Calibrating the Wavelength of the Spectral Sensor easier 34 Irradiance ee Ed 36 Genee eet 37 Saving the New Calibration Coefficients USB Mode sssseseessseeeeseeeeseeeeeeeeseeees 38 Chapter 7 Firmware and Advanced Communications 41 Electrical PINOUT O eta Da anal 41 5 Pin Micro USB Connector essessseesseessessseessressressnnssnnesnnetnnesnntnntnneennosnneeunscnnsonnscnnscnnssennsnns 41 ii 911 00000 000 02 201504 Fo Ocean Ver Opt ics Table of Contents Reprogramming WT 41 Startup est FUNCI N
14. ees EA 41 Upgrading Firmware E 42 Pixel net Le EE EE 42 Hl ee pe Signals EE 42 Timing ConstraiMtS sissies aniei oaa a aAa a A da dudes sates tests aaa eaaa 42 Communication and Interface ANNE ENEE 43 USB2 Orinin oaa aiaa a a a E a a a a aaa aa deed a Ea 43 Spark USB Port Interface Communications and Control Information 43 OVEIVIOW eege nent pus aa cea aiaa ah is canes eet ed ieee cee aad eden 43 USB Information EE 44 Protocol Design Ocean Binary Protocol cece eeeaeeeeaeeseneeeseaeeesaeeseeesseeees 44 Spark Command Protocol cccccccceceeeeeeeeeeeeeeeeeeeeaeeeeaeeseeeeeseaeeeseaeeseaeeseeeeeseaeeesaeeseaeeseeneess 44 Message LAYOUT s zzis stars cas savage seecti eege eege EEN eege cus AS ada SALAO Gotan 45 e Le 45 Ne EE 48 CheckSUM sue sets std ahaa cas teas advaseasseddaaly do Sae Sd da do ved tea CER RO avai desde Manse cas EEN eg 48 eE E EN EE EE ch gegen eege eh ond ia E ties Deche ege eech 48 OT E 48 Message ul 49 General Device CommandS viievsccseeeesetetevsedeeseessezeheessavenhs bvdatevsereeieiepetaneefiardecds heet Eege 51 SPECIOSCOPIC COMIMANAS E 53 Temperature CommandS asaesanisusridsenascansezun anaa anaa aaa E a aa 58 Chapter 8 Spark OEM VIS and Spark DET VIS Advanced Use 61 Advanced Product Details sais nega sad sd is Sa aa 61 COMME CHIVILY iresten eegene 62 MNO Naa RIDE RNA E RE UERR T 65 911 00000 000 02 201504 iii an t cecan Optics Table of Conte
15. for example or a calculator that performs third order linear regressions Note If you are using Microsoft Excel choose Tools Add Ins and check AnalysisToolPak and AnalysisTookPak VBA Calibrating the Wavelength of the Spectral Sensor 34 gt Procedure Perform the steps below to calibrate the wavelength of the Spark 1 Place the spectral sensor operating software into Quick View Scope mode and take a spectrum of your light source Adjust the integration time or the A D conversion frequency until there are several peaks on the screen that are not off scale Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum intensity Record the pixel number that is displayed in the status bar or legend located beneath the graph Repeat this step for all of the peaks in your spectrum Use the spreadsheet program or calculator to create a table like the one shown in the following figure In the first column place the exact or true wavelength of the spectral lines that you used 911 00000 000 02 201504 a Ocean In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and in the fourth column calculate the pixel number cubed Independent Values Computed Dependent from the Regression Variable Variables Output True Wavelength nm Pixel Pixel amp Pixel Wath Difference 2
16. measurements range Fantastic value Our lowest cost spectral Accessibility for high volume or sensor cost constrained applications Ease of use Spark VIS plug and play with Education remote control on micro USB connectivity UAVs and new applications For more information on applications or for a little inspiration please visit our Applications page at http www oceanoptics com application 2 911 00000 000 02 201504 2Ccean CES ics Specificationsjc Summary 1 Introduction Specification Spark VIS Spark OEM VIS Spark DET VIS Spectroscopic Spectral Range 380 to 700 nm Optical resolution 4 5 to 9 0 nm FWHM 1 2 wavelength Signal to noise ratio 1500 1 Stray light 0 3 to 1 across range Electronic A D resolution 14 bit N A Power consumption 250 mA O 5 30 mA O 5 VDC VDC Connector micro B USB 12 pin 0 5 mm 14 pin 7 x 2 power and pitch ribbon connector signal cable Detector Detector Panavision ELIS1024 linear silicon CMOS array Integration time 10 ps to 10s Pixels 1024 Physical Operating temperature 10 to 60 C Relative humidity 0 to 85 non condensing Dimensions L x W x H 53 3 x 36 4 x 38 4 x 22 6 x 18 42 x 9 65 x 19 9 mm 10 2 mm 9 53 mm Weight 159 3 89 lt 1g 911 00000 000 02 201504 Ocean une 0 Product Versions Spark VIS Spark OEM VIS Spark DET VIS Core product
17. off Detectors do not have a completely linear response As they approach saturation typically their efficiency reduces When your spectral sensor is manufactured e Stray Light Correction on or off an advanced user option that allows you to set a 1 or 2 term polynomial correction for stray light correction 2 Installation and Setup Controls that appear in this window depend on the device model You can add and remove acquisition controls from this window Quick View and Device Response In Quick View mode formerly Scope mode the spectra that are displayed have an arbitrary y axis given in counts This is the raw signal from the detector and is proportional to the voltage induced by the light falling on the detector It is very important to realize that this is uncalibrated data and that a counts signal does not represent a particular power or energy from one wavelength to the next Because the response of the detector is linear twice the counts at a particular wavelength do indicate that the amount of light at that wavelength has doubled relative to another wavelength However a small peak relative to a big peak does not indicate that there is less or more light at a particular wavelength relative to another in absolute terms To understand the true relationship you need to do a relative measurement including relative irradiance or if you want a quantified result an absolute irradiance measurement The relative effic
18. served Checksum Immediate type length Byte 24 wii Byte 39 unused Byte Byte Byte Byte No Byte Byte Byte Byte Byte 40 41 42 43 payload 44 Byte 59 60 61 62 63 0x14 0x00 0x00 0x00 Checksum 0xC5 OxC4 0xC3 0xC2 Bytes remaining Footer 50 911 00000 000 02 201504 Fo Ocean t Opt Ics 7 Firmware and Communications Response Header Byte 0 Byte1 Byte2 Byte3 Byte4 Byte5 Byte 6 Byte 7 0xC1 0xCO 0x00 0x10 0x01 0x00 0x00 0x00 Start bytes Protocol version Flags Error number Byte 8 Byte9 Byte 10 Byte 11 Byte 12 Byte 13 Byte 14 Byte 15 0x00 0x10 0x10 0x00 X X X X Message type 0x00101000 Regarding user specified Byte 16 Byte 17 Byte18 Byte19 Byte20 Byte21 Byte 22 Byte 23 0x00 0 Be Checksum Immediate S type length Byte 24 Byte 39 unused Byte Byte43 Payload Byte Byte Byte Byte Byte Byte Byte 42 2048 2092 Byte 2108 2109 2110 2111 40 41 bytes of 2107 spectral 0x14 0x08 0x00 0x00 data Checksum 0xC5 OxC4 0xC3 0xC2 Bytes remaining Footer General Device Commands Message Type Purpose Input Data Output Data Notes 0x000 000 00 Reset N A N A Forces a reset of the device Wait 1 second before reopening the port 0x000 000 01 Reset Defaults Clears certain per
19. 23 25 6 812123132249326E 8 1 764565531630069E 4 1 1123123533351986E 9 6 Click on each of the calibration coefficients displayed in the Wavelength Coefficients pane of the USB Programmer screen and enter the new values acquired in Step 5 of the Calibrating the Wavelength of the Spectral Sensor section in this appendix Repeat Step 6 for all of the new values Click on the Write Coefficients to Device button to save the information and then exit the Utility software The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the Spark 911 00000 000 02 201504 39 ay t cecan Optics 6 Calibration 40 911 00000 000 02 201504 Chapter 7 Firmware and Advanced Communications The following chapter contains information on advanced programming and communications This applies to the Spark VIS format which may be programmed through its USB connection Electrical Pinout 5 Pin Micro USB Connector Listed below is the pin description for the Micro B USB connector Pin Signal Name Description 1 Muss USB power connection 5V Pin orientation 2 D Data Line 12345 3 D Data Line 4 N C No connection 5 GND Ground Reprogramming Mode The Spark VIS contains a feature to allow an existing or failed application to be replaced with a new one Refer to the section Upgrading Firmware for more details about how Reprogramming the Application is accomp
20. 34 calibration coefficients saving in USB mode 38 Color setup 14 communication interface 43 connect Spark in OceanView 19 D document audience v purpose v summary v 911 00000 000 02 201504 Index E electrical pinout for micro USB connector 41 firmware 41 firmware upgrade 42 Fluorescence setup 12 H hardware setup 7 installation software 6 Spark 5 irradiance calibration 36 Irradiance setup 13 ISO certification vi measurement techniques 10 mechanical diagrams 31 memory chip 33 micro USB connector pinout 41 nonlinearity correction 37 65 Index O OceanView 7 Acquisition Parameters 19 connect to Spark 19 dark measurement 23 launch 17 main screen 18 methods 22 product key 26 projects 22 Quick View 20 reference measurement 23 save data 21 Saved Data panel 21 schematic view 24 wizards 23 packing list 5 pixel binning 42 product applications 2 features 2 introduction 1 product related documentation v Q Quick View 20 R Reflectance setup 11 repairs 28 reprogramming mode 41 S save data 21 Saved Data panel 21 schematic view 24 servicing 28 setup 5 Absorbance 10 66 cecan Optics ay t Color 14 Fluorescence 12 Irradiance 13 optimize 15 Reflectance 11 Transmission 11 SMA Adapter accessory 9 SPARK DET VIS 61 SPARK OEM VIS 61 specifications 3 29 start up test 41 T timing signals 42 Transm
21. 4 uV e Output voltage due to dark current 6 mV s Sensitivity 6 74 V lux s at 555 nm Clock frequency pixel read rate 150 kHz 911 00000 000 02 201504 61 f3Ocean ho 7 i 8 Firmware and Advanced Communications O oO o Wos For more information on the ELIS1024 detector and for acquisition timing charts see the detector data sheet http www isgchips com pdf PanavisionSVI_ELIS 1024A Datasheet pdf All three versions of the Spark contain a thermistor Murata NCP15XW223J03RC embedded into the detector board Please see http www murata com products catalog pdf r44e pdf for more information on this part Connectivity The Spark OEM VIS device must be connected to the user s driver electronics via a ribbon cable Molex part number 21020 0121 pitch 0 5mm Details for the output pins on the cable are given in the table below Pin Function Description 1 TH1 Connected to Thermistor Murata NCP15XW223J03RC which is tied to the Device Ground 2 GND Device Ground 3 Vout Buffered Video out signal 4 GND Device Ground 5 5Vin 5V Device Supply 6 Shutter 7 M1 8 Mo 9 RM All control and drive signals operated per ELIS 1024 datasheet 10 Reset 11 Data 12 CLK The Spark DET VIS device must be connected to the user s electronics via a pin connector Samtec part number TFML 107 02 L D A with mating connector either SFML 107 02 L D A or SFML 107 02 L D Details f
22. 53 65 175 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 579 01 0 06 696 54 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 751 47 1669 2785561 4649101309 751 27 0 19 5 Use the spreadsheet or calculator to calculate the wavelength calibration coefficients In the spreadsheet program find the functions to perform linear regressions e If using Quattro Pro look under Tools Advanced Math e If using Excel look under Analysis ToolPak 6 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After executing the regression you will obtain an output similar to the one shown below Numbers of importance are noted 911 00000 000 02 201504 35 Cegn Optics ay t 6 Calibration Regression Statistics Multiple R 0 999999831 R Square 0 999999663 R Squared Adjusted R Square 0 999999607 Standard Erro
23. 9 00 02 00 would get pixels 5 1 9 2 in that order 0x001 020 80 Get and send partial corrected spectrum Acquires a baseline corrected spectrum and returns just the pixels specified by the last partial spectrum specification If no specification has been set since the device was started this will return a NACK with a device not ready error Only the specified pixels will be in the response so the caller must have the specification available to decipher the results The pixels will be packed into the immediate data portion of the header if they fit otherwise they are appended after the bytes_remaining field as an extended payload Averaging and boxcar are applied if necessary before the values are returned Partial spectrum modes 0x0001 Acquire every Nth pixel starting with pixel 0 The only parameter for this mode is spacing which is how much to increment the pixel index after each pixel 0x0002 Acquire a band of pixels Three parameters are expected starting pixel index inclusive increment can be positive or negative but not 0 count includes a starting pixel cannot be larger than the total count The count is a maximum fewer pixels will be returned if there are not enough pixels in the current binning mode to satisfy this parameter 0x0003 Acquire pixels by index This allows up to 10 pixel indices to be given in any order If any index is invalid for the active resolution t
24. able provided with the Spark Connect the spectral sensor to the USB port on your computer using this cable See Hardware Setup for more in depth information You will find more information about using the Spark with OceanView in Chapter 3 Spark Operation with OceanView For more detailed information please see the OceanView manual see Product Related Documentation Software Installation The Spark is designed to work with OceanView 1 5 and above It is not compatible with SpectraSuite or older software You can use OceanView on the following operating systems Software Windows Apple Linux SS 2000 XP Vista 7 8 8 1 OceanView Vv I4 OSX Version 10 5 Any version released for or later on Intel an x86 or amd64 platform processor since 2010 Note Your computer must be connected to the Internet while the software is being installed For advice on how to install software while offline please contact our Technical Support team 6 911 00000 000 02 201504 Kocen Opt ICS 2 Installation and Set up About OceanView OceanView is the latest generation of operating software for all Ocean Optics spectral devices It is a Java based spectroscopy software platform that operates on Windows Macintosh and Linux operating systems The software can control any Ocean Optics USB device OceanView is a user customizable advanced acquisition and display program that provides a real time inte
25. age 2 Boxcar width 8 mm T 2 04 05 06 07 08 Baseline Correction 5 x B E 1 1 1 oo A E b Chromaticity Parame ters Stray Light Correction H i i Property Value Ag dominant waveL 480 0 nm Trigger Mode On Dei Ca chroma 182 129 Ty tint 33 33 W whiteness 721 5 X Axis Wavelent hap hue angle 96 497 degrees n e d p excitation purity 0 479 t mpre en 500 550 650 700 L 143 663 Resolution 1024 p E D 261 34 teed n V Ensina Y 260 76 S 1 2347 08 z 422 58 Wavelength nm 480 078 sl at 20 608 Saved Data 2 Spark Color Measurement in OceanView 14 911 00000 000 02 201504 Kocen e Optics 2 Installation and Set up Typical Color Applications e LEDs characterization e Lighting monitoring e Screen color matching Optimizing Your Setup Typical Ocean Optics miniature spectrometers split the incident light from the sample into its component wavelengths using a diffraction grating this requires a finite path length and gives a lower limit to the volume of a grating based spectrometer The Spark instead uses a solid state optical sensor to capture the spectrum No grating no path length no volume Owing to the nature of the device the following should be considered e The whole 1 mm x 8 mm aperture on the front of the Spark must be entirely illuminated This ensures that each detector pixel receives light The SMA adaptor SPARK ACC SMA ADAPTOR projects light from the fiber acros
26. app 4 adt 46D app SO S20 zap S t SED 600 620 6dt G6D GEL 700 Subt4 10 20 38 670 txt 18 Mar 2015 10 20 38 000 Wavelength nm mm cics 5 Saved Data Panel 1 Saved Files List of saved files currently in the saved directory Arrange by name or date 2 Preview Shows a preview of the saved spectra time series or appended series saved data can be stepped through acquisition by acquisition using the controls above the saved files list File Path Set the file directory 4 Overlay Set the previewed spectra as an overlay on the active view Notes Enter notes about the saved spectra Notes are saved with the same file name as a separate tsv file These can be viewed or edited with any text viewer such as notepad Projects and Methods OceanView makes it easy to save and load projects and methods We define a project as a measurement set up made with a particular spectral device If the software cannot find the device it will load this as a method and prompt the user to select a substitute device from those selected Kl Click to save a project Alternatively select File Save Project from the menu Saves all view and schematic parameters to a single ASCII file Pa Load a project or method 22 911 00000 000 02 201504 HMOCECN beer Sor 2 Installation and Setup Spectroscopy Application Wizards A Click this button to set up a measurement using simple step by step wizards A large range
27. at caused the error Set by device Error Number field contains reason for NACK e Bit 4 exception occurred Indicates that although the message itself was valid the device encountered a hardware problem that may have invalidated the result Error Number will be set to explain if possible Set by device e Bit 5 The message protocol used by the caller is deprecated If set an older version of the protocol has been detected version less than 0 1100 Set by the device Error Number 0x0000 OxFFFF Only set by the device Indicates whether the previous request was successful Only set to be non zero if at least one of the following flags is set NACK or exception Only one value can be set even if multiple errors were detected Values e 0 Success no detectable errors e 1 Invalid unsupported protocol e 2 Unknown message type e 3 Bad checksum e 4 Message too large e 5 Payload length does not match message type e 6 Payload data invalid e 7 Device not ready for given message type e 8 Unknown checksum type e 9 Device reset unexpectedly e 10 Too many buses Commands have come from too many bus interfaces e 11 Out of memory Failed to allocate enough space to complete request e 12 Command is valid but desired information does not exist e 13 Int Device Error May be unrecoverable e 100 Could not decrypt properly e 101 Firmware layout invalid e 102 Data packet was wr
28. d that the product must be returned the representative will issue an RMA number 2 Package your product ideally in the original packaging and return it to Ocean Optics along with the RMA number that you received Note For RMA returns under warranty we will organize and pay for shipping both ways For accidental damage you only pay to have the product delivered to your closest Ocean Optics or OOI Distributor Office Upon careful examination we ll advise you with an estimate When your product is ready it will be returned to you Servicing To keep your instrument in tip top shape we recommend yearly wavelength recalibration You can do this yourself if you have appropriate tools or we can do this for you Contact your local representative to find out more about service availability and cost We offer the following services e Wavelength Calibration for Spark VIS and Spark OEM VIS e Absolute Irradiance Calibrations for these two models for certain applications 28 911 00000 000 02 201504 Chapter 5 Technical Specifications Specifications Specification Spark VIS Spark OEM VIS Spark DET VIS Optical and Spectroscopic Optical Range 380 to 700 nm Optical resolution 5 to 10 nm FWHM 1 4 wavelength Signal to noise ratio 1500 1 Readout noise 3 RMS counts Dynamic range for single acquisition 4600 1 Dynamic range of system 5 x 10 1 Stray light 0
29. don t quite go to plan hopefully you ll find some answers below If not dont hesitate to contact us and our Tech Support team will leap into action Some typical questions are answered here For more information consult the FAQs on the Ocean Optics website at http oceanoptics com faq connected the USB cable and started OceanView but don t see my Spark attached Use the Rescan button in the Device Manager to rescan for attached devices If the device still doesn t appear try reinstalling the driver files Device Manager In Use Location Device Actions Disconnect Add Device Rescan Simulate device if none found V Automatically connect to devices ext Rescan Button am having trouble installing the drivers what should do Hardware device driver installation is usually seamless on Microsoft Windows operating systems and should happen in the background when you connect your Spark to a computer with the software installed and with a connection to the Internet However some Windows systems require a bit more care when connecting your spectral sensor for the first time 911 00000 000 02 201504 25 Fo Ocean 4 Troubleshoot OPTICS Note The driver files may take several minutes to install The driver will only load if the computer is connected to the Internet and the time taken to install will depend on Internet connection speed If OceanView doesn t recogni
30. e behavior to fit your measurement needs from single snap shots to a continuous stream of data over time Saved Data Panel The saved data panel lets you see your data as it is saved and preview data It also makes it simple to add overlays of saved data to your screen 911 00000 000 02 201504 21 Ocean emma RS eee OceanView 1 4 55 Licensed A AUAN el il views tiago atirado XNLBAS 1 H H 1 H 1 H o ST E TE EE EE 1 H 1 1 1 1 1 1 1 H 1 H 1 j H Schematic Window C i H i H 1 H H H 1 H H H H H H 1 H WI un E EE joe SS SRS aya eS SR eps ste io ele in SR pea eee J ea re eye a iain ESSE eeh l 4 EEN be Intensity counts Acquisition Group Window Et T T T T T T T T 360 380 ago o 460 430 sqo 540 540 560 1 1 i 1 i 1 1 1 1 1 Wavelength nm Saved Data em Header ii A A Users alexandergordon oceanview records DI d m Timestamp Frame Leet ial E Auto scale Use as Overly geg Baseline corre Stop averagint BUE Hai gt gt i Number of Pix econ File Name Creation Date Subt4_10 09 58 721 txt 18 Mar 2015 10 09 58 000 Notes Subt4 10 12 12 028 txt 18 Mar 2015 10 12 12 000 Subt4_10 12 13 240 txt 18 Mar 2015 10 12 13 000 SparkVIS_10 03 31 859 txt 18 Mar 2015 10 03 31 000 Intensity counts a 5 18 Mar 2015 10 12 14 000 Subt4_10 07 25 954 txt 18 Mar 2015 10 07 26 000 60 pp
31. e irradiance and relative calibrations and more information on these is located in the OceanView manual see Product Related Documentation 36 911 00000 000 02 201504 ean ptics e t O 6 Calibration Note For absolute measurements it is essential that the lighting and geometry of the setup is exactly the same when absolute irradiance calibrating the device as when taking a measurement Any variation from the calibration setup moving of fibers light source or device altering the device acquisition parameters or altering the background lighting will nullify the calibration Please contact our Technical Support team for advice on how to perform an appropriate calibration Nonlinearity Correction A linear device is one in which the output is proportional to the input For example if the input is doubled then the output would double In the case of a spectral device the output is given in counts and the input in amount of light photons We expect doubling the amount of light should double the counts at each pixel assuming a zero baseline It may not be easy to accurately double the amount of light by adjusting the light source but it should be easy to double the amount of light collected by doubling the integration time So for a constant amount of light the signal in counts should vary proportionally with the integration time The detector and the A D converter in a real device will have some degree of nonlinearity
32. ean Optics provides software that can do this calculation OOINLCorrect The experiment for deriving these coefficients requires a stable light source that can saturate the Spark across a significant portion of its spectral range e g LS 1 The software changes the integration time steadily and determines the variation in intensity versus time and generates the polynomial coefficients that will make this linear Saving the New Calibration Coefficients USB Mode Ocean Optics programs wavelength calibration coefficients unique to each Spark onto an EEPROM memory chip in the Spark You can overwrite old calibration and nonlinearity coefficients on the by using the following procedure gt Procedure To save wavelength calibration perform the following steps 1 Ensure that the Spark is connected to the computer and that you have closed all other applications 2 Point your browser to http oceanoptics com support software downloads and scroll down to Utility Programs Select OBP Setup Tool 3 Save the files to your computer and extract them from the zip file Double click the file to run the software 5 Click on the desired Spark device displayed in the Devices pane 38 911 00000 000 02 201504 6 Calibration vw Devices v Serial Number Y Non Lineanty 0 9923220276832581 220 2209930419922 4 232120045344345E 5 0 2320999950170517 3 2343299045944907E 12 3 243999890401028E 5 1 2231232693338256E 10 1 4299999475479126 32123
33. ed by the USB interface are divided up such that it is possible to request a spectrum and query the status of the device or provide other commands while waiting for the spectrum to be returned see USB 2 0 for the USB command set USB Endpoints any data query on either OUT will cause a response on the corresponding IN ERT OUT EP1 IN EP2 OUT EP2 IN Spark USB Port Interface Communications and Control Information Overview The Spark is a spectral sensor that can communicate via the Universal Serial Bus USB This section contains the necessary command information for controlling the Spark via the USB interface This information is only pertinent to users who wish to not utilize Ocean Optics drivers to interface to the Spark Only experienced USB programmers should attempt to interface to the Spark via these methods 911 00000 000 02 201504 43 be Sor 7 Firmware and Communications Note After start up or reset wait 1 second before sending commands to the Spark The Spark performs measurements at start up to correct for baseline USB Information Ocean Optics Vendor ID number is 0x2457 and the Product ID is 0x4200 Protocol Design Ocean Binary Protocol The Spark VIS uses the same binary protocol as the Ocean Optics STS and QEPro spectrometers which is commontly referred to as Ocean Binary Protocol The binary command protocol for the Spark spectral sensor has the following design characteristics e Provides
34. eld or the Payload as appropriate for the length of the data being returned regardless of the message type Host programs decoding this protocol should always be capable of checking both areas in any response Checksum A 16 byte block must appear after the payload if any to contain checksum data This block is required even if no checksum is used according to the Checksum Type field or if the checksum does not require the full 16 bytes The unused parts of the block are for padding to ensure the message length is consistent This protocol does not support checksums longer than 16 bytes e g SHA but the intent of the checksum is to detect bit errors not to prevent tampering or to provide cryptographic assurance If a checksum is used it will be computed starting with the start byte of the header and continuing through the last byte of the payload The length of the checksum and footer will not be included in the checksum i e for MD5 which includes the total data length as a salt value Footer After the checksum block a 4 byte footer is provided The footer has a constant value of 0xC5C4C3C2 Do not reverse the order of the footer Message Types The binary protocol divides up the 4 29 billion possible message types into categories and subcategories in a hierarchy The most significant bits represent the more abstract categories while the least significant bits represent subcategories and the commands The 32 bit message type is
35. ery quickly On most machines getting speeds above 200 requires a USB hub to be between the Spark and the computer since the hub attempts to read the data back from the device more often than the machines native full speed USB chips do The transaction translator of a high speed hub can Increase the Spark throughput by reducing some of the inactive time periods where the Spark is waiting to send its data Approximate Maximum Scans Per Second Binning Mode USB No Hub USB High speed Hub 0 1024 70 80 1 512 120 2 256 160 3 128 250 450 Timing Signals Timing Constraints The following table defines the timing limits of firmware controlled aspects of the Spark 42 911 00000 000 02 201504 fo Ocean t Opi Ics 7 Firmware and Communications Parameter Value Notes Minimum integration time 10us Integration period error is approximately 1 at 10us and less than 0 1 for integration times 2 1ms Minimum cycle time 13 3ms Cycle time includes integration time readout and transmission via USB This time may only be guaranteed at the minimum integration time USB bandwidth USB 2 0 Full Speed 12mbit s Communication and Interface USB 2 0 The primary data interface between the Spark and a host computer is via USB On the microprocessor the interface is USB 2 0 Full Speed which provides 12Mbit s of bandwidth The maximum update rate is 75 Hz The endpoints provid
36. evel of craftsmanship and reliability for years to come No other manufacturer offers such a solid guarantee of quality and reliability The Ocean Optics 3 Year Warranty applies to Ocean Optics equipment excluding OEM configurations purchased on or after July 1 2010 The warranty covers parts and labor needed to repair manufacturing defects that occur during the warranty period We also will cover the costs of shipping warranty related repairs from our customers to Ocean Optics and from us to our customers ISO Certification Ocean Optics the industry leader in miniature photonics has been certified for ISO 9001 2008 certification applicable to the design and manufacture of electro optical equipment since 2009 vi 911 00000 000 02 201504 f Ocean gece bout This ana Compliance A WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures FCC COMPLIANCE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio
37. ference click to update the stored reference measurement EH Dark click to update the stored dark measurement 911 00000 000 02 201504 23 AL cean Sem 2 Installation and Setup Schematic View e Div Mult_6 Relative RelativeView_ Ref Subt 5 Constant 7 The schematic view is a graphical interface that allows you to move from device through to processed data There are a few basic components to consider Devices each device will appear separately Right click to open a menu that can generate an acquisition control a TEC if applicable and add other device controls a Acquisitions each device can output one acquisition per detector channel Right click to open menu Nodes nodes are the building blocks of the schematic view They are all various functions that take data in and provide an output To make a node right click on the schematic background Each node can be configured by double clicking on the node To join nodes press ctrl click and Mult_6 E drag windows Views Are a type of view that displays data To generate a new view right click on the schematic background More information about schematic view including detailed descriptions of the available nodes can be found in the OceanView Installation and Operation Manual and in the help section of the OceanView software 24 911 00000 000 02 201504 Chapter 4 Troubleshooting Sometimes things
38. h and safety Color Color measurements are typically taken in reflectance transmission or irradiance setups the only difference being that color parameters such as Chromaticity Coordinates or Correlated Color temperature are calculated from the spectrum The Spark is ideal for color measurements in a number of experimental setups it captures a much richer dataset than the RGB filter diode systems which base their calculations on only a handful of wavelengths as the Spark captures data across the entire visible spectrum Perceived color is strongly dependent on lighting conditions as objects tend to appear different colors under different illuminations With the right lighting two objects can appear to be identical in color even if the reflected spectral power distributions differ an effect called metamerism If the lighting changes however the colors will look different This makes controlled lighting conditions essential in many environments such as photography studios or store lighting and accurate color analysis important ece OceanView 1 4 55 Licensed f Acquisition Group Window D EM Emissive Colour e E a i t RARA E Main Controls gt ZS eg Pal E DJ gt u Il Running g a S Data Update Rate GT RN RR RR O ae E E Integration Time Aut S Soll ms 5 z E S Ss a IB RA Scans to Aver
39. he pixel value for that index will be OxFFFF This is to prevent the specification from becoming invalidated by changing resolutions All parameters including the mode are 16 bit values LSB first and they are all unsigned except for the increment for mode 2 The returned values should include any and all averaging and boxcar compensation as specified separately and the baseline will be compensated as usual 54 911 00000 000 02 201504 2Ccean CES ics 7 Firmware and Communications Message Type Purpose Input Data Output Data Notes Examples Specification mode 1 spacing of 4 pixels 0 4 8 12 16 Specification mode 2 starting at pixel 100 every pixel for 10 pixels 100 101 102 0x01 00 04 00 109 0x02 00 64 00 01 00 0A 00 Specification mode 3 for pixels 5 8 500 and 375 0x03 00 05 00 08 00 F4 01 77 01 0x001 10010 Setintegration Unsigned N A Input is 4 bytes for time in us Order is time us 32 bit LSB MSB integer No reply The minimum is 10 0x001 102 80 Get pixel Single byte Returns a single byte indicating the binning factor binning mode 0x001 102 81 Get maximum Single byte Returns a single byte representing the binning factor largest binning factor that may be used 3 The minimum is assumed to be zero 0x001 102 85 Get default Single byte Returns the startup binning factor as a binning factor s
40. her Accessories sssseeeeeessenseensserestnsstnsstnsstnsstesstenstennnensnnnsnensnnnnnnnnt 9 Measurement Techniques Typical Set Up ENNEN 10 le Eelere 10 Common Absorbance Applications eseesseesseeeeeee seee ie se eeeentttne ttnn ttnnntnnntnnnennntn nennen nsen nnnn 10 Reflectance amp TranSiMiSSion cccccssccceesecceeeeeeeceeeeeeneeeeeeeeeeeesaeaeeeeneaeeeenseeeeeeneeaeeeeneeaeeseneaes 11 Common Reflectance Applications esseesseesseeeeeeseee ie eeetetnetennttnnttnnttnnuttnntnnnnnnnennnnn nnen nenn 11 Common Transmission Applcatons 11 FIUOFESCENCE EE 12 Common Fluorescence Applications re ereeaere rare aaa nana 12 Lg gs 0 TE ue 13 E lee 14 Optimizing OUR SC Upa aa SACRA Rd ES Rad 15 911 00000 000 02 201504 Table of Contents Chapter 3 Spark Operation with OceanView cessseeeeeeeeeeeeeees 17 Eeer RR ee eee E eee eee ne eee 17 ener 17 OceanView Main Screen EEN 18 Connect the Spark in OceanView cccecceceteceececeeeeecaeeeeaaeeeeneecaeeeeaaeseeaeeseeeesaeseeaaesseneessaees 19 set Acquisition Parameters za ieasesssadasiaos ascents snteseeesteeussdakiesrseaectuksseexusrtaedecevsaceetintewcniiheabers 19 Quick View and Device Response rear aaaaanaeeeara arara nana aannaans 20 Continuous and Single Acquisitions cccccceeececeeeeeceeeeeeeaeeeeeeeseaeeeeeaeseeaeeseeeeseaeeesaeeeeneeseaees 20 Save Data saias niin di sian aii Ha A
41. how on startup The OceanView Welcome Screen Version 1 5 911 00000 000 02 201504 17 ean Orin e Quick View Displays the spectrum in Quick View mode showing raw unprocessed data This is uncorrected for instrument response vs wavelength Quick View shows you a live shot of what the Spark is seeing From Quick View you can launch application wizards or construct your own method 2 Installation and Setup e Load a Saved Project Loads a previously saved project Click Restore Last Session to reload the schematic and views as they were when the software was last closed e Spectroscopy Application Wizards Use this function to set up a measurement using simple step by step wizards A large range of applications is available OceanView Main Screen No matter what route you take on start up you will soon end up on the OceanView main screen This is where you can set and view measurements save and load data and save projects ER OceanView 1 4 54 Licensed AMBUSH april O Acquisition Group Window cbt 1LAQQAQAYW VAKADA afb aah G ki UR Running i H i H i i i Data Update Rate o msec E n po Seer ees Pare neta iP eee Saas pa anes seme ip Sie Seale ea Te Integration Time Automatic sol ms Scans to Average 20 Boxcar width gig Baseline Correction Stray Light Correction Trigger
42. hrough USB for Spark VIS Chapter 8 Spark OEM VIS and Spark DET VIS Advanced Use Gives connection and specification details on OEM and DET devices Offers additional data for ELIS detector circuitry and electronic specifications Product Related Documentation You can access documentation for Ocean Optics products by visiting our website at http www oceanoptics com Select Support gt Technical Documents then choose the appropriate document from the available drop down lists 911 00000 000 02 201504 g2Ocean About This manua OPTICS Document for Document Location OceanView software http oceanoptics com wp content uploads OceanViewlO pdf Device driver issues http oceanoptics com wp content uploads Correcting Device Driver Issues pdf Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your state of the art products and how they function located at http oceanoptics com glossary Document Version Document Number Version 911 00000 000 02 201504 First release Warranty Our 3 Year Warranty covers Ocean Optics miniature fiber optic spectrometers spectral sensors light sources and sampling accessories regardless of the application from manufacturing defects It also covers fibers and probes for a full 12 months http oceanoptics com services exclusive 3 year warranty This comprehensive warranty ensures you of the highest l
43. iability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual Table of Contents ABOUE Ba TEE UC EE v Document Purpose and Intended Audience eee arenas v Document SUMMAN aiia ec Gl een Vaii a ea i iri ta a v Product Related Documentation v Document Versi Meaus 2 st8 tient ere acd cat aangaraucn Scales aa SR ee pentevanqaaicuanaceaat aateerent eg vi Way sect ahs ceca eek Eege end eee steed Eege ab ara ees eee ated dat vi SO COMNICANON ia cient GR ee cerca meek Cede anes UC OS Cotes aca vi Chapter 1 InttoduCHON asas passas saidas 1 Product Introduction se O e NT EH 1 Product Features and Applications enei oinaan RAA EENE EEEE REE ERRE 2 Specifications Summary EE 3 leiere Ee 4 Chapter 2 Installation and Setup ccccecseseeeeeeeeeeeeeeeeeeeeeeeeeeneeeeees 5 Whats Inthe BOX sas isa exert eines Sege dead Ee CEL ces E RUI RL Er Age AE deetdece tacit 5 Spark WISE EE 5 software I MStAN ALON EE 6 About OCC ANVIEW EE 7 Hardware Setups 22 sxitcicveiarh amp tedetecevtesni eee pay taste dude do cotas Rida ended eevee deepened t AES 7 Available AGCOSSONGS ees 8 ie Eeer 8 Light Sources and Ot
44. iency of converting light to detector signal varies significantly across the range of a spectral sensor Many things impact this including the responsiveness of the detector quantum efficiency and the efficiency of optical components Each configuration of a spectral device has a unique response curve referred to as the instrument response Continuous and Single Acquisitions There are two sets of controls for taking or pausing acquisitions The set on the Acquisition group window allows you to control each device individually The set on the top bar is a global control that will allow you to start and pause all devices currently attached 20 911 00000 000 02 201504 3Ocean SS saturn seus po Aquire data continuously Kl Take a single acquisition and then pause I Pause all acquisitions Save Data M Configure Saving set saving parameters and file type file directory and file naming convention Once selected the file directory will persist until changed fal Start saving data Turns red when save is active If saving data continuously click when red to stop saving Will only activate saving for acquisitions attached to that particular view kl Global Save Activates all configured saves across all views Use to save data from multiple devices at the same time By default OceanView will save data as a single snapshot acquisition By configuring the save you can set the sav
45. information so that the host does not need to know the state of the device to read the message e Contains a distinct header and footer to fully bracket transfers e Provides an abstract interface to the device All timing is represented in standard units rather than clock divisors A specific outcome is achieved via a single mechanism e Stores calibration information wavelength nonlinearity coefficients etc in distinct commands rather than EEPROM slots Spark Command Protocol There are two types of messages in this protocol e commands that do not return any information e queries that cause the device to return information When developing a device driver that will communicate with the Spark the fact that some messages generate a response including a status indication and others do not can cause design problems The simplest approach to creating a driver for this protocol is to have all message types generate a reply This allows a single message read to be performed after every message write and if the response indicates an error then the driver can recover immediately rather than finding the error later when it expects a response to some new query The flags field in the message header starting at byte offset 4 has an acknowledgment ACK requested bit bit 2 If this bit is set to 1 for every command and left at O for every query then all communications with the Spark will become predictable read write transacti
46. ing systems all you need to do is install the Spark operating software then unplug and replug in the device Technically the driver files for Linux simply give nonprivileged users permission to use newly connected hardware There isn t any long term harm to plugging in the device before installing the software How do determine whether my Windows computer is 32 bit or 64 bit Errors can occur if you download the wrong version of software for 32 bit or 64 bit computers To verify your computer version for most Windows computers go to the Properties window under Computer or My Computer If no version is listed then your system is a 32 bit For more information see the Microsoft Frequently Asked Questions at http windows microsoft com en us windows 32 bit and 64 bit windows 1TC windows 7 911 00000 000 02 201504 27 Optics Go cem 4 Troubleshooting Product Upgrades Repairs and Servicing Occasionally you may find that you need Ocean Optics to make a change or an upgrade to your system To facilitate these changes you must first contact Customer Support and obtain a Return Merchandise Authorization RMA number Please contact Ocean Optics for specific instructions when returning a product Repairs Sometimes accidents happen If you need to return your Ocean Optics product for repair here is what to do gt Procedure 1 Contact us to speak to an Ocean Optics representative about the problem If it is determine
47. ingle byte 0 by factory default may be overridden as an acquisition parameter while device is powered up and default may also be reset see below 0x001 10290 Set pixel Takes a single byte as the binning binning factor factor to use for this bus until the device is reset 0x001 10295 Set default Zero bytes Can take either zero bytes clearing binning factor or single back to factory default or one byte byte indicating new default mode as arguments 0x001 105 10 Set trigger Unsigned N A Input is 4 bytes for time in us minimum delay us 32 bit 5 us Order is LSB MSB integer No reply 0x001 200 00 Get scans to Unsigned Gets the number of scans 1 5000 to average 16 bit average together before returning the integer spectrum 0x001 20010 Set scans to Unsigned Sets the number of scans to average average 16 bit 1 5000 Argument is an unsigned 16 integer bit integer LSB first The spectrum LSB first response will still be 16 bits per pixel The average will round to the nearest integer with any exact half being rounded up 911 00000 000 02 201504 55 7 Firmware and Communications Cosa Message Type Purpose Input Data Output Data Notes 0x001 210 00 Get boxcar Unsigned Gets the boxcar width being applied to width byte all spectra Valid range is 0 15 0x001 21010 Set boxcar Unsigned Sets the boxcar width to apply to all width byte spectra Valid range is 0
48. ission setup 11 troubleshooting 25 Linux systems 27 Mac systems 27 Windows systems 26 U upgrades 28 USB port interface 43 V versions 4 W warranty vi Wavelength Calibration about 33 Wavelength Calibration Data File 5 Wavelength Calibration Data Sheet 5 what s new vi 911 00000 000 02 201504
49. lished Start up Test Function The Spark will verify its firmware on startup If the firmware is intact the device will operate normally If it appears to be damaged or corrupted then the device will enter reprogramming mode This start up test protects the Spark from being rendered unusable due to failed attempts at reprogramming 911 00000 000 02 201504 41 fo Ocean TeFirmware and Communications OPTICS Upgrading Firmware Upgrading the Application in the Spark can be accomplished using the USB interface An Application is distributed by Ocean Optics in an OBP file Upgrading the application is as simple as passing this file to the Spark via one of the aforementioned interfaces The USB interface will ensure that proper flow control will take place Pixel Binning Internally the device has 1024 pixels As the resolution decreases the effective pixel area increases When the resolution is set to 512 the photodiodes of pixels 1 and 2 are averaged and output as a single value pixels 3 and 4 are averaged and output as a single value and so on If set to 256 resolution then pixels 1 through 4 are averaged and output as a single value 5 through 8 are averaged and output as a single value and so on Because it is full speed and not high speed 200 scans per second is difficult There are tight timing requirements on the USB host in order to get higher than this rate The Spark will do it but the host has to be trying to get the data v
50. n ASCII string in output for coating Temperature Commands Notes The microcontroller sensor will report values much higher than the detector board thermistor because the microcontroller integrated circuit runs at a higher temperature The Spark contains three memory locations for the temperature sensor as follows 0 Detector Board Thermistor 1 Reserved Internal Use 2 Microcontroller Sensor Temperature 58 911 00000 000 02 201504 Roce Age Onti ICS 7 Firmware and Communications Message Type Purpose Input Data Output Data Notes 0x004 000 00 Get N A byte Request has no input data temperature s nsor count Reply has 1 byte with the number of temperature sensors available 0x004 000 01 Read Unsigned IEEE Provides the temperature in C if temperature byte single calibrated or raw counts for the sensor precision sensor if uncalibrated floating Input is 1 byte for the index of the point sensor to read Reply output is a 4 byte float LSB first of temperature in C 0x004 000 02 Read all N A Array of Each float is the temperature in C if temperature IEEE calibrated or raw counts for the sensors single sensor if uncalibrated The length of precision the array will be equal to the number floating of sensors available points Request has no input data Reply has a 4 byte floating point LSB first for each sensor in the output Units are in C
51. n Data Sheet that contains information unique to your device OceanView reads this calibration data from your Spark when it interfaces to a computer via the USB port Note For the Spark VIS and Spark OEM VIS products please save the Wavelength Calibration Data Sheet for future reference Spark Installation The following procedure provides general instructions for getting your new Spark spectral sensor up and running 911 00000 000 02 201504 5 Ocean Sm 2 Installation and Setup Caution On the first occasion that the software is installed and the first time the Spark is connected to the computer the computer must be connected to the Internet Once the driver software has successfully installed for both the software and the device both products will work offline Be sure to install the software BEFORE connecting the Spark to your computer The software installs the drivers required for spectral sensor installation If you do not install the software first the system will not properly recognize the unit If you have already connected the Spark to a computer running on a Windows platform prior to installing the operating software see Chapter 4 Troubleshooting for information on correcting a corrupt Spark installation gt Procedure 1 Install your spectroscopy operating software by following the installation wizard prompts See Software Installation for more in depth information 2 Locate the micro B USB c
52. n bytes for length short each user string 0x000 003 02 Getuserstring Unsigned String Input is a string index byte 0x000 003 10 Setuserstring Unsigned N A Input is string index followed by data If byte String string data is of zero length user string will be deleted 0x000 010 10 Configures Status LED Byte O is reserved and must always be 0x00 Byte 1 is the pattern to drive the LED 1 LED will blink in an S O S pattern at a high priority this will not override a POST or hard fault indication but will override all others 52 911 00000 000 02 201504 Roce Age Opti ICS 7 Firmware and Communications Message Type Purpose Input Data Output Data Notes 2 LED will fade in and out at a low priority anything but the solid on idle pattern will override this If this byte is anything other than 1 or 2 then the LED will revert to its normal operation 0x000 FFF 00 Put device in N A N A Causes the Spark to accept an OBP reprogramming file provided by Ocean Optics mode Spectroscopic Commands Message Type Purpose Input Data Output Data Notes 0x001 010 00 Get and send N A Pixel This returns the intensity of every pixel corrected values on the detector as LSB MSB as soon spectrum integers as it is available There is no payload in immediately the request The reply has 2048 bytes of payload The pixel intensities are corrected for tempe
53. nge of dedicated accessories for the Spark Note All accessories can only connect to the Spark or Spark OEM via the Base accessory part number SPARK ACC BASE The Base accessory is compatible with both of these product models 8 911 00000 000 02 201504 Kocen e Optics 2 Installation and Set up Spark Base Accessory SPARK ACC BASE e Interface between Spark or Spark OEM and all other accessories this part is compatible with both product formats e Connected to Spark or Spark OEM with two M2 5 screws provided connected to Spark accessories with inbuilt twin magnets e Contains filter holder for 0 5 filters filters themselves sold separately Spark SMA Adapter Accessory SPARK ACC SMA e Magnetically attached to Spark or Spark OEM via Base accessory SPARK ACC BASE sold separately e Couples to SMA905 optical fibers e Can fiber couple to all existing Ocean Optics sampling accessories and light sources Spark Diffuser Accessory SPARK ACC DIFFUSER e Magnetically attached to Spark or Spark OEM via Base accessory SPARK ACC BASE sold separately e Recommended for free space measurements Light Sources and Other Accessories Ocean Optics supplies a large range of other accessories for use with all of our spectral sensors and spectrometers These are all fiber coupled accessories and therefore you may only connect these to the Spark via the SMA Adaptor accessory part
54. nging the power of spectroscopy to the sensor market The Spark also offers users full spectral information in applications previously dominated by RGB photodiode devices Spark Spectral Sensor Spark and Spark DET 911 00000 000 02 201504 fo Ocean The benefits to the customer are huge the Spark s small footprint allows for a wide range of applications integration into hand held devices on line process monitoring mounting on UAVs and its low cost brings the power of spectroscopy both to the bulk market and to the individual user The Spark is capable of detecting light in the 380 to 700 nm wavelength range making it ideal for color absorbance and fluorescence measurements in both educational and industrial environments This novel technology will allow our customers to explore a previously inaccessible world of spectroscopy measurements We are eager to find out where you go and what you are able to do with this revolutionary device Product Features and Applications Feature Specification Good for Visible wavelength range 380 to 700 nm Color PAR fluorescence and absorbance measurements Highly compact 15g including electronics Integration into handheld and 3 8g without electronics portable devices or onto process lines lt 1g for detector Thermally stable 10 to 60 C operating range Analysis in harsh environments High resolution 4 5 to 9 0 nm 1 2 across gt 80 element color
55. nts iv 911 00000 000 02 201504 About This Manual Document Purpose and Intended Audience Thank you for choosing Ocean Optics We hope that you ll be delighted with your decision This document provides the users of Spark spectral sensors with instructions for setting up calibrating and performing experiments with their device It also contains detailed technical specifications and information about firmware and hardware integration If you can t find what you re looking for in this document please do not hesitate to contact us at techsupport oceanoptics com or via www oceanoptics com Document Summary Chapter Description Chapter 1 Introduction Introduces the product features Contains descriptive information about the Spark and its accessories Gives an overview of features applications and specifications for all users Chapter 2 nstallation and Setup Provides installation and set up instructions for users of the Spark VIS product Chapter 3 Spark Operation with OceanView Outlines how to make measurements with OceanView software Chapter 4 Troubleshooting Covers commonly encountered user difficulties Chapter 5 Technical Specifications Contains an overview of specifications and product mechanical diagrams Chapter 6 Calibration Contains details on calibration Chapter 7 Firmware and Advanced Communications Provides details on advanced communication protocol t
56. number SPARK ACC SMA sold separately These accessories include a Fibers Light Sources Integrated Sampling Systems Cuvettes including microfluidic cuvettes Filter holders and filters including low pass band pass and high pass Oooo For advice on which accessories may be required for your application or for help in ordering these parts please contact Ocean Optics 911 00000 000 02 201504 9 JOcean Gas sem Measurement Techniques Typical Set ups The Spark in conjunction with Ocean Optics light sources and sampling accessories can be used for many different measurement techniques One of the key advantages of modular fiber optic spectroscopy is that you can change components of the system without having to buy a whole new system Here we show a range of typical visible set ups for basic spectroscopy techniques You ll find lots more information about measurement techniques at www oceanoptics com measurementtechnique Absorbance Absorbance is typically a relative measurement comparing the spectrum from the sample to that of a reference Absorbance is commonly used for concentration measurements and for identifying components in mixtures Connect the Spark to our cuvette accessories via the SMA Adaptor accessory to take a liquid sample Absorbance measurement or mount it directly against the sample with a light source on the opposite side for solid sampling bluLoop Quartz Cuvette Light Source with Sample
57. of applications is available pOceanView Set up a Spectroscopy Application with our Spectroscopy Wizards Reflectance O Absolute Irradiance Application Wizard Window 1 Z 2 Dark and Reference Measurements Dark and reference measurements are commonly used in spectroscopy e Dark Measurements subtract a background signal from the spectrum This can be considered the removal of a constant error Typically this is done when the light source is off to remove any background from the ambient environment hence the name dark e Reference Measurements make the signal relative to the reference Consider this a normalization of the signal against a reference Typically this is taken with a reference sample and the light source turned on This lets you look at the relative spectral change compared to a reference sample Most often you will set up your measurement with the reference and dark through the application wizards The wizards will prompt you to take your reference and darks Alternatively you can use the quick dark and quick reference features Once a dark and or reference measurement has been set you can update it with the controls on the top bar of the view O Quick Reference click to take a reference and set up a new view After clicking it will prompt the user to take a dark 9 Quick Dark click to take a dark measurement and sets up a new Quick View minus dark view Q Re
58. on bytes Reply has up to 4096 bytes whatever has been stored previously intended for 1024 x 4 byte floats If nothing has been stored the reply will have NACK bit set in flags 0x001 820 02 Get irradiance N A Unsigned Request has no payload Geh Se Reply is a 4 byte integer LSB first 9 indicating total number of 4 byte floais to be returned in payload of reply to Get Irradiance Calibration including 0 0x001 820 03 Get irradiance N A IEEE single Request has no payload calibration precision h collection area Reply If a collection area has been set its returned as a 4 byte float LSB first If not defined it returns a NACK 0x001 820 11 Set irradiance Up to 4096 N A Request has up to 4096 bytes in calibration bytes payload Sending a zero length buffer will delete any irradiance calibration from Spark No reply 0x001 820 13 Set irradiance IEEE single N A Request has a 4 byte float Otherwise calibration precision no payload to erase value completely collection area Sending a zero length buffer will delete any collection area previously stored No reply 0x001 831 00 Getnumber of N A Unsigned stray light byte coefficients 0x001 831 01 Get stray light Unsigned IEEE single Input is the order of the coefficient to coefficient byte precision retrieve 0x001 831 11 Set stray light Unsigned N A Input is the order of the coefficient to coefficient byte IEEE set followed by an IEEE single single precision float preci
59. ong size not 64 bytes e 103 hardware revision not compatible with firmware e 104 Existing flash map not compatible with firmware e 255 Operation Response Deferred Operation will take some time to complete Do not ACK or NACK yet 46 911 00000 000 02 201504 QOcen Age Opti ICS 7 Firmware and Communications Offset Field Size Valid Values Notes Bytes Bytes 8 Message 4 0x00000000 Each message type represents a command See Type Message Types OxFFFFFFFF 12 Regarding 4 0x00000000 Arbitrary host defined data Any response generated by the device will have the same value in its OxFFFFFFFF Regarding field This can be used by the host to match responses to re que Spark if transactions are split up 16 Reserved 6 For future expansion 22 Checksum 1 0x00 0x01 Valid types Type da e 0 no checksum must still provide a block of 16 bytes after the payload but they can be zero e 1 MD5 fully fills the 16 byte checksum block 23 Immediate 1 0x00 0x10 Total number of bytes used in the Immediate Data Data field see below Length 24 Immediate 16 Provides an alternative to specifying a payload so Data commands with small operands can fit within a single USB packet If this field is used the number of bytes containing valid data must be set in the Immediate Data Length field and there is no payload If a payload is used this field is ignored 40 Bytes 4 0x00000000
60. ons The immediate reply allows the host driver to avoid changing its state until it has received confirmation that the last operation succeeded or failed This makes driver design much easier than the alternative It is recommended that an Spark protocol driver implement two functions e send command to device which takes a message type and an optional payload and returns a simple pass fail result based on the ACK or NACK flag in the response This should set the ACK requested bit in every message it emits 44 911 00000 000 02 201504 4 qo cem e Opt ICS 7 Firmware and Communications e query device which takes a message type and optional payload and returns a payload e g a byte array which can be NULL if the response was a NACK This must not set the ACK requested bit because to do so would generate a spurious ACK in addition to the expected response By using these two functions to encapsulate all transfers to the Spark the programming model is kept very simple Message Layout All multi byte fields are little endian LSB first Each message in the binary protocol is laid out as follows 1 A 44 byte header 2 An optional payload 3 A 16 byte checksum block 4 A four byte footer The header checksum and footer are 64 bytes total For simple messages the command or response is embedded in the header so only a single packet is required For more complex messages the header and footer add a single USB packet as o
61. or the output pins on the connector are given in the table below Pin Function Description 1 5Vin 5V Device Supply 2 Vout Buffered Video out signal 3 Shutter All control and drive signals operated per ELIS 1024 datasheet 4 GND Device Ground 5 M1 See pin 3 description 62 911 00000 000 02 201504 F Ocean t Opt ICs 7 Firmware and Communications Pin Function Description 6 GND Device Ground 7 MO See pin 3 description 8 RM See pin 3 description 9 Reset See pin 3 description Connected to Thermistor Murata NCP15XW223J03RC which is tied 10 TH1 to the Device Ground 11 Data See pin 3 description 12 CLK See pin 3 description 13 GND Device Ground 14 GND Device Ground Using the information given above these two formats of the Spark may be easily integrated into your system Ocean Optics recommends using a core Spark VIS unit to test your application before attempting an integration For assistance with your integration please do not hesitate to contact Ocean Optics 911 00000 000 02 201504 63 q Cegn Optics A Q 8 Firmware and Advanced Communications 64 911 00000 000 02 201504 A Absorbance setup 10 accessories 8 Base 9 SMA Adapter 9 Acquisition continuous 20 single 20 Acquisition Parameters 19 B Base accessory 9 box contents 5 C calibration 33 irradiance 36 preparing for 34 procedure
62. ptics website See Product Related Documentation 26 911 00000 000 02 201504 fo Ocean V Opt ICS 4 Troubleshooting Remove the Unknown Device from Windows Device Manager gt Procedure 1 Open Windows Device Manager Consult the Windows operating instructions for your computer for directions if needed 2 Locate the Other Devices option and expand the Other Devices selection by clicking on the sign to the immediate left Note Improperly installed USB devices can also appear under the Universal Serial Bus Controller option Be sure to check this location if you cannot locate the unknown device 3 Locate the unknown device marked with a large question mark Right click on the Unknown Device listing and select the Uninstall or Remove option 4 Click the OK button to continue A warning box appears confirming the removal of the Unknown Device Click the OK button to confirm the device removal 5 Disconnect the Spark from your computer 6 Replug the Spark into your computer The system should now able to locate and install the correct drivers for the USB device 7 Plug in the USB device The system is now able to locate and install the correct drivers for the USB device Apple Mac Operating Systems Since there are no device files for the Spark in a Mac operating system you should not encounter any problems if you installed the device before the operating software Linux Operating Systems For Linux operat
63. r0 125540214 Observations 22 Intercept Coefficients R Error Intercept 190 473993 M o First coefficient X Variable 1 0 36263983 001684745 X Variable 2 1 174416E 05 8 35279E 07 X Variable 3 2 523787E 09 2 656608E Second coefficient Third coefficient 7 Record the Intercept as well as the First Second and Third Coefficients Additionally look at the value for R squared It should be very close to 1 If not you have most likely assigned one of your wavelengths incorrectly Keep these values at hand for Saving the New Calibration Coefficients USB Mode lrradiance Calibrations Irradiance calibrations and relative irradiance calibrations quantify the spectra by translating the signal incident number of photons to a known output This can be either absolute when using a light source of known output power or relative when using a light source of known spectral shape thereby correcting for instrument response function but not output power It is used widely in remote sensing light metrology and anywhere where you wish to characterize the incident light source Irradiance calibrations are not required for many techniques because these measure the relative signal changes with respect to the sample and a reference not changes in the light source itself You can find out more about irradiance calibration techniques at http oceanoptics com measurementtechnique irradiance OceanView has wizards that will step you through absolut
64. ration coefficients must be saved to the user s electronics Wavelength Calibration This section describes how to calibrate the wavelength of your spectral sensor As the wavelength for all units will drift slightly as a function of time and environmental conditions Ocean Optics recommends periodically recalibrating your Spark About Wavelength Calibration To calibrate the device the following equation needs to be solved this shows that the relationship between pixel number and wavelength is a third order polynomial 4 I Gp QP GP Where A the wavelength of pixel p I the wavelength of pixel O G the first coefficient nm pixel G the second coefficient nm pixel C the third coefficient nm pixel p the Pixel Number from 0 911 00000 000 02 201504 33 6 Calibration CC Optics Fa t5 The values for 7 and the three G need to be found Wavelength Calibrating the Spectral Sensor Preparing for Calibration To recalibrate the wavelength of your spectral sensor you need the following components A light source capable of producing spectral lines Note Ocean Optics HG 1 Mercury Argon lamp is ideal for recalibration If you do not have an HG 1 you need a light source that produces several at least 4 6 spectral lines in the wavelength region of your spectral sensor A Spark spectral sensor An optical fiber use the SMA adapter accessory A spreadsheet program Excel or Quattro Pro
65. rature drift and fixed pattern noise 0x001 011 00 Get and send N A Pixel This returns the raw ADC output for raw spectrum values every pixel on the detector as LSB immediately integers MSB as soon as it is available There is no payload in the request The reply has 2048 bytes of payload 0x001 02000 Get partial Returns a specification for partial spectrum mode spectrum retrieval see below If no specification has been set since the device was started this will return a NACK indicating no value available 0x001 020 10 Set partial spectrum mode Input is as follows partial pixel mode formats Mode 1 interval spacing 0x01 00 II II Mode 2 band 0x02 00 SS SS II Il CC CC Mode 3 selected 0x03 00 XX XX YY YY ZZ ZZ Where SS SS is the starting pixel index 16 bits LSB first II Il is the increment positive or negative but not zero also 16 bits LSB first and CC CC is the total number of pixels including the starting pixel cannot be larger than the total pixel count 16 bits LSB first 911 00000 000 02 201504 53 7 Firmware and Communications Orr Optics Message Type Purpose Input Data Output Data Notes XX XX is the index of the first pixel to grab YY YY is the index of the second pixel to grab ZZ ZZ is the third and so on for up to ten pixels Each pixel index is 16 bits LSB first For instance a payload of 0x03 00 05 00 01 00 0
66. reject wavelengths above and below the range of detection adding extra filtering at the source may help to reduce stray light further You ll find more useful information including a useful glossary of terms on our website at http www oceanoptics com glossary For advice on your application please do not hesitiate to contact our Technical Support team at techsupport oceanoptics com 911 00000 000 02 201504 15 aOcean Zinstalatonan Seup AE E 16 911 00000 000 02 201504 Chapter 3 Spark Operation with OceanView Overview The following sections provide enough information to perform general functions with your Spark VIS spectral sensor and OceanView software For more detailed information about OceanView see the OceanView Installation and Operation Manual see Product Related Documentation Launch OceanView Once you have installed your software and connected your spectral sensor you are ready to display your measurement data using OceanView Launching OceanView differs depending on your operating system and where you have placed your OceanView program files For PCs running Microsoft Windows the default location is Start Programs Ocean Optics OceanView OceanView For Mac computers the default location is the Applications folder When you first start OceanView the Welcome Screen appears A Welcome Screen 26 Quick Load a Spectroscopy View Saved Project Application Wizards Restore Last Session W S
67. rface to a variety of signal processing functions With OceanView you have the ability to perform spectroscopic measurements such as absorbance reflectance and emission control all system parameters collect and display data in real time and perform reference monitoring and time acquisition experiments Consult the OceanView manual for hardware requirements when using OceanView see Product Related Documentation Hardware Setup The Spark spectral sensor connects to a computer via the USB port When connected through a USB 2 0 port the spectral sensor draws power from the host computer eliminating the need for an external power supply The Spark VIS like all Ocean Optics USB devices can be controlled by our OceanView software This is a Java based spectroscopy software platform that operates on Windows Macintosh and Linux operating systems gt Procedure Follow the steps below to connect the Spark to a computer via the USB port 1 Install the spectral sensor operating software on the destination computer 2 Locate the micro B USB cable provided with the Spark 3 Insert the micro B connector end of the cable into the side of the Spark Insert the larger USB Type A end of the cable into the USB port of the computer 4 Connect any Spark accessories see Available Accessories Your computer must also be connected to the Internet the first time that the Spark is plugged into the computer to install the driver files If this is no
68. s the entire aperture For free space measurements users must take care with their measurement geometry e The spectral response will be sensitive to the angle of incidence of the illumination Ideally fix the Spark in place relative to the source Alternatively use a diffuse source The ideal set up is a diffuse light source at a normal angle of incidence perpendicular to the slit If it is not fixed you may notice small changes in the spectral shape e Use the Diffuser accessory SPARK ACC DIFFUSER sold separately to help create a diffuse illumination of the entire aperture See Spark Accessories for more information e Because the Spark s optical train is not based on diffraction gratings there is no impact of the grating on the instrument response However because of the unique solid state technology there may be an element of fixed pattern noise similar to PDA detectors visible in the raw Quick View mode e As with all spectrometers it is important to use a processed mode such as absorbance or relative irradiance to generate results Quick View mode is there to help you set up the measurement See the Ocean Optics glossary for more on instrument response and Quick View mode e To help minimize stray light use a light source that only emits across the range of interest This is true for all spectrometers but especially the Spark because of the unique sensor technology Visible LEDs are ideal While the Spark does have filtering to
69. sion 0x001 860 00 Spectral sensor N A Up to 116 Request has no data get hot pixel bytes indices Reply has up to 58 x 2 byte integers 1 integer per pixel index If nothing has been stored the reply will have NACK bit set in flags 911 00000 000 02 201504 57 7 Firmware and Communications SEE Message Type Purpose Input Data Output Data Notes 0x001 860 10 Spectral sensor Up to 116 N A Request has up to 58 x 2 byte integers set hot pixel bytes for pixel indices indices No reply 0x001 BOO 00 Get bench ID N A String Request has no input data Reply has up to a 32 byte ASCII string in output for bench ID 0x001 B01 00 Get bench N A String Request has no input data sanal number Reply has an ASCII string in output for serial number 0x001 B02 00 Get slit width N A Unsigned Request has no input data mMICTOnS 16 bit Reply is a 2 byte integer LSB first for the integer micron value 0x001 B03 00 Get fiber N A Unsigned Request has no input data diameter 16 bit Reply is a 2 byte integer LSB first for the microns integer micron value 0x001 B04 00 Get grating N A String Request has no input data Reply has an ASCII string in output for grating 0x001 B05 00 Get filter N A String Request has no input data Reply has an ASCII string in output for filter 0x001 B06 00 Get coating N A String Request has no input data Reply has a
70. sisted values including default baud rate and pixel binning mode Once they re cleared the device will reset and should come up in the factory default mode Does not erase serial number bench alias calibration or user strings 911 00000 000 02 201504 51 7 Firmware and Communications SEE Message Type Purpose Input Data Output Data Notes 0x000 000 80 Get hardware N A Unsigned This value is sensed from the revision byte hardware itself Request has no payload Reply is a single byte 0x000 000 90 Get firmware N A Unsigned Firmware version as binary coded revision short decimal The same value should be available through the USB descriptor as the bcdDevice field Request has no payload Reply is a 2 byte integer LSB first of the revision 0x000 001 00 Getserial N A String number 0x000 001 01 Get serial N A Unsigned Output data is maximum length of number length byte serial number in bytes 0x000 002 00 Get device N A String User defined name for the device e g alias station number 0x000 002 01 Get device N A Unsigned Output is maximum length of alias in alias length byte bytes 0x000 002 10 Set device alias String N A If string length is 0 alias will be deleted 0x000 003 00 Get number of N A Unsigned User defined strings for storing small available user byte amounts of arbitrary data strings 0x000 003 01 Getuserstring N A Unsigned Output is maximum length i
71. split into three blocks 0xXXX YYY ZZ as follows e XXX top level category or feature 4096 of these may exist e YYY subcategories within the feature 4096 of these may exist for each category e ZZ specific commands for the subcategory 255 of these may exist for each subcategory The top level categories XXX are initially defined as follows e 0x000 General device characteristics 48 911 00000 000 02 201504 F Ocean adro Ics 7 Firmware and Communications e 0x001 Spectral sensor feature control of detector and ADC pixel calibrations and corrections e 0x004 Temperature The subcategories and commands for each of these categories are described in the tables that follow Input and output data lengths that can be computed from the header Bytes Remaining field are not shown All multi byte integer types will be returned in little endian format least significant byte first All data will be carried over Endpoint lor Endpoint 2 IN and OUT unless otherwise stated Message Examples The following is an example of how the Set Integration Time message type 0x001 100 10 can be constructed based on the information provided in this data sheet Header Byte 0 Byte1 Byte2 Byte3 Byte4 Byte5 Byte 6 Byte 7 0xC1 OxCO 0x00 0x10 0x00 0x00 0x00 0x00 Start bytes Protocol version Flags Error number Byte 8 Byte9 Byte 10 Byte 11 Byte 12 Byte 13 Byte 14 Byte 15 0x10 0x00 0x11 0x00 X xX X x
72. surface quality Common Transmission Applications e Turbidity measurements of chemical solutions e Measuring the transmission efficiency of optics and glass 911 00000 000 02 201504 11 2Ocean ZeInstalation andSetup OPTICS Fluorescence A molecule fluoresces if it absorbs light at one wavelength and re emits light at a longer wavelength Many biological samples fluoresce under certain lights In addition fluorescent molecules are often used to track reaction rates and the presence of other non fluorescing compounds Typically the excitation source is applied at 90 to the sample to minimize light from the excitation source reaching the spectral sensor Similarly filters are used to block the lower wavelength light from reaching the detector and masking the weaker fluorescence signal The Spark has a large slit and therefore has good throughput sensitivity making it capable of detecting very low concentrations of fluorescent molecules LLS Light Source NW e ke DcvrL a4o Cuvette with Sample Solution Wa 74 MSP 4 Mirror e Screw Plug LVF UV QR400 7 VIS NIR Optical Fiber Computer Spark VIS Spark ACC BASE Spark ACC SMA e LVF Kit Micro USB Typical Fluorescence Set Up with an LED Excitation Source at 90 Common Fluorescence Applications e Identifying proteins using fluorescent tags e NADH fluorescence e Remote sensing of chlorophyll 12 911 00000 000 02 201504 Koco e Optics 2 Ins
73. t possible see Chapter 4 Troubleshooting Note The driver files may take several minutes to install The driver will only load if the computer is connected to the Internet and the time taken to install these files will depend on Internet connection speed If you installed the spectral sensor operating software prior to connecting the Spark the software automatically installs the Spark drivers If the drivers do not successfully install or if you connected the Spark to the computer before installing the software see Chapter 4 Troubleshooting 911 00000 000 02 201504 7 AL cean Corr 2 Installation and Setup Available Accessories Ocean Optics provides a range of sampling and light source accessories Accessories specifically designed for the Spark are described here they are not provided with the Spark spectral sensor and must be purchased separately See Measurement Techniques Typical Set ups for more information on using accessories with Spark Visit us at www OceanOptics com for a complete list of products available for all of your spectroscopy needs Spark Accessories k 2 VR 2 a A en Ps N The Spark and Spark OEM have points to mount them onto a bench or a surface compatible with M2 5 bolts or screws the mounting points are not threaded The Spark may be mounted towards or away from the surface depending on the sample location These points can be used to simultaneously connect to a ra
74. tallation and Set up lrradiance Irradiance is the technique of measuring the total energy of light at a given wavelength either relative to the spectral output of a known source relative irradiance or in absolute units of power or energy absolute irradiance This is used widely in light metrology color measurement and environmental science Absolute irradiance measurements require an irradiance calibrated spectral device This can be done in the factory for some configurations or by using a calibration lamp in the lab or field Every time a set up is changed the device used must be recalibrated For a more detailed explanation of how to calibrate a device see Chapter 6 Calibration or contact Ocean Optics Use the front mounted diffuser and SMA adapter accessories for irradiance measurements with the Spark Computer Optical Fiber Integrating Sphere Spark VIS Spark ACC BASE Spark ACC SMA Typical Relative Irradiance Set up for Measuring the Light Power Output of an LED Using an Integrating Sphere LED Power Supply Micro USB Incident Light Computer Spark VIS Spark ACC BASE Spark ACC DIFFUSER Radiometrically Calibrated Light Source Micro USB Spark with Diffuser Accessory in Free space Set up 911 00000 000 02 201504 13 Ocean r Optics 2 Installation and Setup Typical Irradiance Applications e Measuring the radiant output of lamps and LEDs e Measuring UV exposure for healt
75. verhead to the transfer Header The message header is structured as follows Offset Field Size Valid Values Notes Bytes Bytes 0 Start Bytes 2 0xC1C0 Chosen for the following reasons e High bits set so not likely to occur in ADC data less than 16 bits wide or message type fields e When concatenated with the tail of a previous message creates a distinct sequence Note Do not reverse this byte order 2 Protocol 2 0x0000 Initially set to 0x1000 The host should only send Version OxFFFF messages known to be supported in the reported version of the protocol Subsets of this protocol may be specified for other devices using a version less than 0x1000 The device may reject messages with a specified protocol it does not recognize 4 Flags 2 0x0000 Bits in this flag are assigned as follows OxFFFF e Bit 0 response to earlier request message type is set equal to request type Set by device e Bit 1 acknowledgment ACK if previous message included request for ACK Set by device e Bit 2 acknowledgment ACK requested Set by Host 911 00000 000 02 201504 45 7 Firmware and Communications q O gedan Optics e Offset Bytes Field Size Bytes Valid Values Notes e Bit 3 negative acknowledgment NACK May be sent if previously sent message type is unknown or otherwise invalid Message type and regarding fields will be set to the type th
76. vices 1 Click on the Device Manager icon 2 Click Rescan The Spark should automatically connect Device Manager Device Actions Disconnect Add Device Rescan Simulate device if none found J Automatically connect to devices Exit Set Acquisition Parameters Acquisition parameters are set using the acquisition group window The window may appear to be minimized when you first start OceanView either expand or open a new window from the menu Window Acquisition Group An active acquisition is required for the Acquisition window to appear Functions available to control in the Acquisition window include the following 911 00000 000 02 201504 19 JOcean Gas cem e Integration Time set the integration time the time over which the detector captures incident light At the end of the integration time the accumulated signal is read from the detector by the electronics e Averaging Signal especially at low levels is often significantly impacted by noise Averaging several spectra together reduces the impact of noise and gives you re a cleaner result However at long integration times averaging can increase the total time of a measurement significantly e Boxcar Boxcar is a form of averaging across pixels It applies a rolling average to multiple adjacent pixels to help smooth the spectral response and reduce the impact of noise e Non Linearity Correction on or
77. ze your device you need to manually install the spectral sensor drivers See your OceanView manual for this procedure Also see the Correcting Device Driver Issues document see_Product Related Documentation I m installing OceanView but need a product key where can find this The product key was sent to the contact e mail on the sales order when you purchased your OceanView license Contact info oceanoptics com for more information You ll need your sales order number quotation number the serial number of the spectral sensor that was purchased with the software and if known the e mail address under which your product key was created to recover your key connected the Spark to the computer before installing my spectroscopy operating software to install the drivers What do do now The steps to take to resolve this issue differ depending on your Computere operating system Microsoft Windows Operating Systems If you connected your Ocean Optics device to the computer prior to installing your Ocean Optics software application on a Windows platform you may encounter installation issues that you must correct before your Ocean Optics device will operate properly Follow the applicable steps below to remove the incorrectly installed device device driver and installation files Note If these procedures do not correct your device driver problem you must obtain the Correcting Device Driver Issues document from the Ocean O

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