Untitled - Masterflex
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1. Inadequate illumination Field of view of left and right eyepiece differ 24 2 Electrical Problem Possible Cause Power supply not plugged in Lamp not installed Lamp does not light User left more than 15 minutes under auto mode Lamp burnt out Inadequate brightness Specified lamp not being used Lamp blows out immediately Specified lamp not being used Connectors are not securely connected Lamp flickers Lamp near end of service life Lamp not securely plugged into socket VII Care and Maintenance 1 Lenses and Filters e To clean lens surfaces or filters first remove dust using an air blower If dust still persists use a soft clean brush or gauze e A soft gauze or lens tissue lightly moistened with the mixture of alcohol and ether ratio alcohol 3 and ether 7 should only be used to remove grease or fingerprints e Use the mixture of alcohol and ether ratio alcohol 3 and ether 7 to clean immersion oil e Use the mixture of alcohol and ether ratio alcohol 3 and ether 7 only to remove immersion oil from objective lenses e Because the mixture of alcohol and ether ratio alcohol 3 and ether 7 is highly flammable be careful handling around open flame e Do not use same area of gauze or lens tissue to wipe more than once 2 Cleaning of Painted or Plastic Components e Do not use organic solvents thinners alcohol ether etc2. e Ambient temperature 15 C 40 C e Maximum relative humidity 75 for temperature up to 31 C decreasing linearly to 50 relative humidity at 40 C e Supply voltage fluctuations Not to exceed 10 of the normal voltage e Pollution degree 2 in according with IEC60664 Installation Overvoltage category 2 in according with IEC60664 e Air Pressure of 75kPa to 106 kPa 2 Input voltage and power Automatic voltage selection works with electrical outlets worldwide It is advised to always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards Using the wrong power cord could cause fire or equipment damage AN In order to prevent electric fluctuation to the instrument electrics always turn the power switch on the instrument off before connecting the power cord This equipment must be used with UL60950 1 Listed power supply E246759 MFR LI TONG ELECTRONICS CO LTD Model LTE50E S2 3 e Input Voltage 12VDC e Input Power 48W e Power adaptor input rating 100 240Vac 47 63Hz 1A Attention The plug of power adaptor is the disconnect device for whole unit To save energy and environmental priciple we recommend you to pull out the plug if you will not use the device in long time This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 th
3. Doing so could result in discolouration or in the peeling of paint e For stubborn dirt moisten a piece of gauze with diluted detergent and wipe clean e For plastic components only moisten a piece of gauze with water and wipe clean 25 3 When Not in Use e When not in use cover the instrument with vinyl dust cover and store in a place low in humidity where mould is not likely to form Store the objectives eyepieces and filters in a container or desiccator with drying agent Note If equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired 4 Warning lable The following warning labels or symbols are found on the microscope Study the meaning of the warning labels or symbols and always use the equipment in the safest possible manner Warning Label Symbol Explanation A Indicates that the surface becomes hot and should not be touched with bare hands A N CAUTION Risk of danger See user manual Proper handling of the microscope will ensure years of trouble free service If repair become necessary please contact your Motic agency or our Technical Service directly 26 Motic CE AO NO 1300901107651 Motic Incorporation Ltd Hong Kong Rm 2907 8 Windsor House 311 Gloucester Road Causeway Bay Hong Kong Tel 852 2837 0888 Fax 852 2882 2792 Motic Instruments Inc Canada 130 4611 Viking Way Richmond B C V6V 2K9 Canada Te
4. Ph1 objective into optical path Position the Phase annular diaphragm slider to Ph1 Position the Phase annular diaphragm slider to PhO when using 4x objective And position the Phase annular diaphragm slider to Ph2 when using 20x Ph2 or 40x Ph2 objective Remove either eyepiece from the eyepiece tube and insert the phase centering telescope instead Fig 25 Rotate the eyepiece of the centering telescope to focus on both the phase plate image of the objective and the annular diaphragm image of the phase slider 19 Fig 25 If the objective phase plate and the annular of the slider do not coincide use the two hexagonal screwdrivers 1 5mm supplied with the microscope Fig 26 to bring the slider annular ring to the centre of the phase plate Fig 27 so that the image of the annular diaphragm is concentric with the phase plate image If the slider annular ring image is diverged from the phase plate image in the objective a low phase contrast image will result Fig 26 Fig 27 For phase contrast microscopy at the maximum contrast use GIF Green interference filter in the optical path Change the annular diaphragm if necessary noted that place it into phase slider in correct direction Fig 28 and use 2 hexagonal screwdrivers 1 5mm supplied to bring the annular ring to the center of the phase slider Fig 29 Put the phase slider back to condenser 20 Fig 28 Fig 29 8 Filter selection Filter hol
5. will enable the user to observe the specimen with both eyes 2 Diopter Adjustment e Diopter adjustment compensates for the differences in vision between the left and right eyes In addition to making observation through both eyes easier this adjustment also reduces the extent to which focusing is lost when the objective magnification is changed In particular this occurs when a low magnification objective is used e Before adjusting the diopter bring a specimen into focus using the 10x objective e Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to the 0 position TER Sr Jua Xi Fig 18 Fig 19 e Position the 40x objective into the optical path and bring the specimen image into focus by turning the coarse and fine focus knobs e Position either the 4x or 10x objective into the optical path Without adjusting the fine and coarse focus knobs turn the diopter rings on the eyepieces so that the specimen images in the left and right eyepieces are focused individually e Repeat the above step twice 16 3 Coarse and fine focusing e Focusing function is realized with the coarse and fine focus knobs at the left and right of the microscope stand e The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs e One rotation of the fine focus knob moves the stage 0 2mm up or down movement The graduation on the fine focus knob is 2 m
6. Motic AE2000 Series Inverted Microscope Instruction Manual SS ltt WWW MOTIC COM MOTIC INCORPORATION LTD CE e U es UL Listed Product E250223 We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods This involves modification to the mechanical structure and optical design of our instruments Therefore all descriptions and illustrations in this instruction manual including all specifications are subject to change without notice Content Nomenclature 1 Application 2 Nomenclature 3 Technical data Setting up the Instrument 1 Working Environment 2 Input voltage and power Assembling the Microscope Input Voltage 1 Installing the bulb 1 1 Halogen bulb 1 2 LED module 2 Filter Holder 3 Mounting the Condenser 4 Installing the Objectives 5 Mechanical Stage 6 Mounting the Eyepieces Microscopic Procedure Interpupillary Distance Adjustment Diopter Adjustment Coarse and fine focusing Coarse focus torque adjustment Change the illumination between Halogen and LED Brightfield Microscopy Phase Contrast Microscopy Filter selection oO ON Oa fF WwW N Auto power off function Photomicrographic Procedure Model AE2000 trinocular 1 Brightfield photomicrography N A A A oo 10 10 12 12 13 13 14 14 15 16 16 16 17 17 18 19 19 21 21 23 23 VI Troubleshoot
7. aint marked on the cover should face the user Fig 8 11 Fig 8 1 1 Halogen bulb The quartz halogen bulb used as a light source has higher luminance and colour temperature than conventional tungsten lamps The luminance of halogen bulb is approximately four times brighter than the conventional tungsten lamps As long as the lamp voltage is kept constant the halogen lamp maintains the same level of brightness and colour temperature regardless of whether it is new or nearing the end of its life span 1 2 LED module The LED module is specially designed to be inserted into halogen bulb socket directly converting halogen illumination to LED illumination LED is more economical and environmental friendly and no heat issue with a long life span 12 2 Filter Holder Fig 9 Fig 10 e Filter holder is located under the lamphouse Fig 9 for easy replacement of the filter Fig 10 3 Mounting the Condenser e Mount the condenser on the dovetail mount of the condenser holder Fig 11 with the aperture diaphragm lever and index marks facing the front and secure it with the clamp screw by 2 5mm hexagonal screwdriver supplied with the microscope Fig 12 N Fig 11 Fig 12 13 e If Phase contrast method will be used insert the Ph annular diaphragm slider with centering hexagonal socket head screws facing the front Fig 13 1 5mm Hexagonal screwdriver Oo Fig 13 4 installing the Objectives e R
8. der could hold two pieces of filters Filter type Procedure ND Neutral Density filter For intensity adjustement in photomicrography For phase contrast and contrast adjustment with GIF Green Interference filter 546nm black and whitetiih For general microscopy and colour Blue filter f photomicrography 9 Auto power off function Pilot Lamp Fig 30 21 There is a auto power off switch locates above the left focusing knob When auto is selected the blue pilot lamp will be turned on It indicates auto power off status Fig 30 If auto is selected the light will automatically turn off after 15 minutes when no user in front of the unit When the user return to the microscope the light will resume immediately with the infra sensor sense someone in front of the microscope Never attempt to switch directly between on and auto The buffer off is necessary between auto power off mode and normal mode 22 V Photomicrographic Procedure Model AE2000 TRI 1 Brightfield photomicrography e The optical path selector knob Fig 31 can be used to select the optical path to either the Binocular tube 100 0 or Binocular tube vertical tube 20 80 observation photo or Path Selector Knob f Fig 31 e Before starting photomicrography check the following The condenser is centered The condenser annular diaphragm is centred The field of view diaphragm is stopped down to sl
9. emove the stage insert from the stage Fig 14 e Install the objectives into the nosepiece so that the magnification increases with clockwise rotation of the revolving nosepiece Fig 15 e Place back the stage insert Metal Stage Insert Fig 14 Fig 15 5 Mechanical Stage e When using a 96 well plate or other petri dish the mechanical stage combined with suited holder should be adopted 3 kinds of Petri dish holder 2 kinds of Heamacytometer holder and 96 well plates holder are available for your opinion e Secure the mechanical stage to the AE2000 plain stage using the two mounting screws Fig 16 located beneath the stage on the right side user facing the front of the instrument 14 Mounting screw Fig 16 The specimen can be moved to the desired position by turning the X axis knob and Y axis knob Mounting the Eyepieces Remove the dust caps from the eyepiece tubes Use the same magnification eyepieces for both the eyes Insert each eyepiece into the eyepiece sleeve and tighten the clamp screws Fig 17 Should the rubber eye guards are to be used fit them in the groove around the eyepiece 1 Clamp screws Fig 17 15 IV Microscopic Procedure 1 Interpupillary Distance Adjustment e Before adjusting the interpupillary distance bring a specimen into focus using the 10x objective e Adjust the interpupillary distance so that both the right and left field of view become one This adjustment
10. icrons Please avoid following action e Never attempt either of the following actions since doing so will damage the focusing mechanism e Rotate the left or right knob while holding the other e Turning the coarse and fine focus knobs further than their limit 4 Coarse focus torque adjustment e To increase the torque turn the torque adjustment ring located behind the left hand coarse focus in the direction of the arrow To reduce the torque turn the ring in the direction opposite off N a 1 Coarse Focus Torque Adjustment Ring 2 Coarse Focus Knob 3 Fine Focus Knob Fig 20 17 5 Change the illumination between Halogen and LED e Attention Unplug the plug in power unit from the power outlet to protect user from electric shock and allow for a sufficient cool down time of the6V 30W halogen lamp before you replace it e Press down lightly and rotate counter clockwise and remove lamphouse cover Fig 21 e Remove the bulb from the socket pinholes gt a Ze Halogen bulb Fig 22 LED Fig 23 e Installing the new bulb into the socket pinholes until it reaches the limited be careful not to tilt it when mounting e If installing the halogen bulb do not touch the glass surface of the lamp with bare fingers Doing so will cause fingerprints grease etc to burn onto the lamp surface reducing the illumination provided by the bulb If surface is contaminated wipe it clean using lens or soft cot
11. ightly just outside the edge of the field of view e For photomicrographic procedures refer to the manual of the specific camera being used 23 VI Troubleshooting Table As you use your microscope you may occasionally experience a problem The troubleshooting table below contains the most frequently encountered problems and their possible causes 1 Optical and Operating Problems Problem Possible Cause Vignetting or uneven brightness in the field of view or field of view only partially visible Bulb not installed properly Filter slider in intermediate position Phase slider not in click stop position Incorrect condenser mounting Aperture diaphragm closed too far Revolving nosepiece not clicked into position Optical path selector lever in intermediate position Mod AE2000 trinocular only Dust or dirt in field of view Aperture diaphragm closed too far Dust or dirt on specimen s surface Image quality No image under phase contrast or details cannot be viewed Brightfield objective being used Phase annular diaphragm not in optical path Phase annular diaphragm and objective phase symbol do not match Slider annular ring image has moved away from the objective phase plate image Thickness of specimen holder is outside the compensating range of objective Eye strain or fatigue Interpupillary distance not adjusted Diopter adjustment not made
12. ine Trinocular 45 incline Ergo incline 45 15 Eyepiece N WF 10X 220 wide field high eye point Nosepiece 4x inclined backwards Stage Fixed Dimensions width x depth 200 x 239mm Stage height 192mm Object guide Verniers with numerical and alphabetic scale X direction numerical scale readable from right to left Y direction alphabetic scale Objectives Plan Achromat 4x N A 0 1 W D 12 6mm Plan Achromat 10x N A 0 25 W D 16 8mm LWD Plan Achromat 20x N A 0 3 W D 4 7mm LWD Plan Achromat 40x N A 0 5 W D 3mm Plan Achromat PH 4x PhO N A 0 1 W D 12 6mm Plan Achromat PH 10x Ph1 N A 0 25 W D 4 1mm LWD Plan Achromat PH 20x Ph2 N A 0 3 W D 4 7mm LWD Plan Achromat PH 40x Ph2 N A 0 5 W D 3mm Condenser N A 0 3 W D 72mm N A 0 4 W D 53mm N A 0 5 W D 28mm Coarse drive 42mm rev Fine drive 0 2mm rev Transmitted 6V 30W Halogen illumination 3W LED Dimension W x Dx H 217 5 x 556 x 497mm ll Setting up the Instrument 1 Working Environment The location should be free from dust moisture chemical vapours and from mechanical vibrations Don t locate the instrument in bright or direct ambient light in front of a lamp or a will lit bright wall Best image will be achieved without significant ambient light Environmental specification e Indoor use e Altitude Max 2000m
13. ing Table 1 Optical and Operating Problems 2 Electrical VII Care and Maintenance 1 Lenses and Filters 2 Cleaning of Painted or Plastic Components 3 When Not in Use 4 Warning lable 24 24 25 25 25 25 26 26 I Nomenclature 1 Application AE2000 incorporates the Color Corrected Infinity Optical System CCIS that offers superior image quality for the transmitted and reflected illumination in the application of Living organism and tissue observation Petri dish observation Live cell in culture observation 2 Nomenclature Model AE2000 Lamp House Filter Holder Clamp Screw Eyepiece Phase Slider Condenser Stage Diopter Adjustment Ring Light Intensity Control Knob Model AE2000 1 5mm Hexagonal screwdriver Objective Nosepieces Auto Power Off Switch Fine Focus Knob Coarse Focus Knob N Coarse Focus Torque Adjustment Model AE2000 TRI lt A Vertical Photo Port ann E Optical Path _ Selector Knob Az ERGO Tube i Ven Model AE2000 Ergo 3 Technical data Technical data AE2000 AE2000 TRI AE2000 Ergo Optical system Color corrected infinity optics Total Magnification 40X 400X Eyepiece tubes 360 rotatable Siedentopf with upper and lower position upper position offers approx 40 mm extra viewing height Interpupillary distance 48 mm to 75 mm Binocular 45 incl
14. is device must accept any interference received including interference that may cause undesired operation lll Assembling the Microscope h Installing the bulb e In order to prevent electric shock always turn the power switch off Fig 1 and unplug the power cord Fig 2 before replacing the bulb e To remove lamphouse cover press down lightly 1 and rotate counter clockwise 2 Fig 3 Power Cord Power Switch Fig 1 Lamp house Cover Fig 3 10 Firmly insert the bulb into the socket pinholes Fig 4 until it reaches the limit be careful not to tilt the lamp when mounting Fig 5 Fig 4 Fig 5 Halogen Bulb e When installing the halogen bulb be care not to touch the glass surface of the bulb with bare fingers to avoid fingerprints grease etc Surface pollution can burn the bulb and reduce the illumination provided by bulb If surface is contaminated wipe it clean using lens tissue or soft cotton Clamp screw 1 5mm Hexagonal screwdriver Fig 6 Fig 7 LED module e Firmly insert the LED module into the socket pinholes until it reaches the limit This is Motic patent design to exchange LED module and halogen bulb on the same socket directly e After the LED module installation Fig 6 secure it with the clamp screw by 1 5mm hexagonal screwdriver supplied with the microscope Fig 7 e Return lamphouse cover to original position and rotate clockwise to lock into place The white p
15. l 1 877 977 4717 Fax 1 604 303 9043 Motic Deutschland GmbH Germany Christian Kremp Strasse 11 D 35578 Wetzlar Germany Tel 49 6441 210 010 Fax 49 6441 210 0122 Motic Spain S L Spain Pol gon Industrial Les Corts Cami del Mig 112 08349 Cabrera de Mar Barcelona Spain Tel 34 93 756 6286 Fax 34 93 756 6287 Website http www motic com E mail digital info motic com Motic China Group Ltd China Motic Building Torch Hi Tech Industrial Development Zone Xiamen P R C Tel 86 0592 562 7866 Fax 86 0592 562 7855 2007 2010 Motic China Group Co Ltd All rights reserved Motic is a registered trademark and service mark of Motic China Group Co Ltd Microsoft Windows logo is a registered trademark of Microsoft Corporation All other trademarks are the property of their respective owners Design Change The manufacturer reserves the right to make changes in instrument design in accordance with scientific and mechanical progress without notice and without obligation Updated 2010 10
16. ton e Return lamphouse cover to original position and rotate clockwise to lock into place The white paint marked on the cover should face the user e The same halogen bulb holder can be used for both LED illumination module 18 Brightfield Microscopy Set the Phase annular diaphragm slider in the centre position without phase annular diaphragm Fig 24 Bring the specimen image into focus The condenser aperture diaphragm is provided for adjusting the numerical aperture N A of the illuminating system of the microscope It is important because it determines the resolution of the image p contrast depth of focus and brightness Stopping down the aperture diaphragm will lower the resolution and brightness but increase the contrast and depth of focus By stopping down the N A of the condenser to 2 3 of the N A of the objective a good image of suitable contrast will be obtained Fig 24 Phase Contrast Microscopy Phase contrast objectives are labelled Ph PhO Ph1 Ph2 For phase contrast microscopy be sure to use the annular diaphragm that has the same phase label as the objective despite of the magnification of the objective Move the aperture diaphragm lever on the condenser to fully open position Always fully open the aperture diaphragm for phase contrast microscopy If aperture diaphragm is closed it will obstruct the annular diaphragm and the phase contrast effect cannot be obtained Bring the 10x
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