Eppendorf Reference® 2
Contents
1. 2 Keep the control button fully pressed and turn the lower part approximately 30 to the right The lower part is automatically released from the upper part 3 Open the lower part fit the pipette key 4 included in the delivery package onto the lower part Hold the cylinder and unscrew it from the lower part See the next figure 26 Maintenance Eppendorf Reference 2 English EN The lower part consists of the following pu 4 5 6 1 2mL 10 mL piston mount 4 Piston with seal 2 Piston spring 5 Cylinder and tip cone 3 Upper part of the lower part 6 Filter sleeve with protection filter in the tip cone Before assembling the pipette first install the piston mount the piston spring and the piston in the upper part of the lower part Further assembly is as shown in the figures Carry out the procedure in reverse order Carry out a gravimetric test of the systematic and random errors This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 6 4 3 Multi channel lower part 6 4 3 1 Loosen and open the 10 uL 100 uL and 300 uL multi channel lower part us Germany Pender 100 QJ Itm Rp rr Gano 1 Set the selection dial for spring loading to On 2 and Slide the lever on the lower part to the left or right This separates the lower p2. 1 Lever Loosens the multi channel lower part 2 Housing lid Removable housing lid with retracted ejector rail 3 Multi channel lower part Al The multi channel lower part is freely rotatable The lower part 2 does not detach when it is rotated The outer channels are labeled with the numbers 1 and 8 or 12 The multi channel version has a piston for each channel to ensure that a smaller number of pipette tips can also be mounted The lower part can be converted from an 8 to a 12 channel qi version with the same volume range The multi channel lower part can be opened enabling you to replace or remove individual channels EX ET eppendorf Reference 2 4 Latches One right latch and one left latch to unlock the housing lid 5 Selection dial On Off Switches on or off the spring loading of the tip cones 6 Tip cones Spring loaded tip cones optimize the force required to attach and eject pipette tips 7 Pipette tips We recommend using epT I P S Product description Eppendorf Reference 2 11 English EN 2 4 Materials NOTICE Aggressive substances may damage the Reference 2 and accessories Check the chemical resistance before using organic solvents or aggressive chemicals Please note the cleaning instructions Your can find further information on chemical resistance on the included CD or on our webpage www eppendorf com The Reference
3. Liquid with high vapor pressure and or different density Wet the tip several times and adjust the pipette for the liquid used Pipetting was too quick gt Move the control button slowly The tip is withdrawn from the liquid too quickly Withdraw the tip slowly from the liquid with a time delay approx 3 seconds Liquid aspirated with blow out and dispensed with blow out Piston dirty or damaged Repeat dispensing correctly Clean piston grease slightly and or replace The tip cone is damaged Replace the lower part or channel The O rings of the tip cones are damaged Replace the O rings only 100 uL 300 uL multi channel The control button jams and does not move smoothly The piston is dirty The seal is dirty Seal defective The pipette is blocked gt Clean the lower part Replace the protection filter on 2 mL to 10 mL models The adjustment seal has been removed the adjustment display has been changed The pipette has been adjusted for a different liquid gt Adjust the pipette for the liquid used No spring loading action of the tip cone when pipette tips are inserted Spring loading action is blocked by a locking ring Spring loading is switched off multi channel Use of a2 mL to 10 mL pipette gt Remove the locking ring again Switch on spring loading No remedy available The tip cone of
4. 2 uL 20 uL Yellow 2 uL 25 0 0 10 1 5 0 03 Increment 2 uL 200 uL 0 02 uL 53 mm 10 uL 1 2 0 12 0 6 0 06 20 uL 1 0 0 2 0 3 0 06 10 uL 100 uL Yellow 10 uL 3 0 0 3 0 7 0 07 Increment 2 uL 200 uL 0 1 uL 53 mm 50 uL 1 0 0 5 0 3 0 15 100 uL 0 8 0 8 0 2 0 2 20 uL 200 uL Yellow 20 uL 2 5 0 5 0 7 0 14 Increment 2 uL 200 uL 0 2 uL 53 mm 100 uL 1 0 1 0 0 3 0 3 200 uL 0 6 1 2 0 2 0 4 30 uL 300 uL Orange 30 uL 2 5 0 75 0 7 0 21 Increment d eae 150 pL 41 0 41 5 40 3 40 45 0 2 uL 55 mm 300 uL 0 6 1 8 0 2 0 6 100 uL 1000 uL Blue 100 uL 3 0 3 0 0 6 0 6 Incremeng PESE 500 uL 41 0 45 0 40 2 41 0 1 uL 71mm 1000 uL 0 6 6 0 0 2 2 0 33 34 Technical data Eppendorf Reference 2 English EN Reference 2 single channel variable Model Test tip Testing volume Eppendorf AG error limits epT I P S color code Error Volume range Length Systematic error Random error mL mL 0 25 mL 2 5 mL Red 0 25 mL 4 8 0 012 1 2 0 003 Increment 0 25 mL 2 5 mL 0 002 mL 115 mm 1 25 mL 0 8 0 010 0 2 0 0025 2 5mL 0 6 0 015 0 2 0 005 0 5 mL 5 mL Purple 0 5 mL 2 4 0 012 0 6 0 003 Increment 0 1 mL 5mL 0 005 mL Oni 2 5 mL 1 2 0 030 0 25 0 006 5 0 mL 0 6 0 030 0 15 0 0075 1mL 10 mL Turquoise 1 0 mL 3 0 0 030 0 6 0 006 Increment 1mL 10 mL QO 165 mm 5 0 mL 40 8 40 040 40 2 40 010 10 0 mL 40 6 40 060 4
5. these sizes does not have spring loading action 18 Troubleshooting Eppendorf Reference 2 English EN Regularly check the precision and accuracy of the Reference 2 to prevent dispensing errors You can use the PICASO software to determine the maximum permissible systematic and random errors If the pipette is used at an extremely high altitude it must be adjusted to the ambient air pressure A Standard Operating Procedure SOP for checking pipettes can be found on the Reference 2 CD and on our webpage www eppendorf com Maintenance Eppendorf Reference 2 19 English EN 6 Maintenance NOTICE Aggressive substances may damage the Reference 2 and accessories Check the chemical resistance before using organic solvents or aggressive chemicals Please note the cleaning instructions Your can find further information on chemical resistance on the included CD or on our webpage www eppendorf com All single and multi channel lower parts are wear parts Clean them after contamination use of aggressive chemicals and or heavy stress If the lower parts are worn or damaged replace these parts Faulty dispensing results are sometimes due to lack of maintenance 6 1 Cleaning NOTICE Damage to device from unsuitable cleaning fluids or sharp or pointed objects Unsuitable cleaning fluids can damage the device Never use corrosive cleaning fluids strong solvents or abrasive polishes Check the co
6. 2 components that can be accessed by the user are made of the following materials Component Material External surfaces of the upper part Improved polypropylene PP Polycarbonate PC Polyetherimide PEI Stainless steel Exterior and interior of lower parts Improved polypropylene PP Polyvinylidene fluoride PVDF Polyetherimide PEI Polyphenylene sulfide PPS Polyetheretherketone PEEK Polytetrafluoroethylene PTFE Ethylene propylene diene rubber EPDM Silicone Steel stainless steel and spring steel Pipette tip Material epT I P S Polypropylene PP epDualfilter T 1 P S filter Polyethylene PE 12 Product description Eppendorf Reference 2 English EN 2 5 Storing the pipette The pipette can be safely stored in a pipette carousel or a wall mounting device 2 5 1 Pipette carousel A pipette carousel 4923 is available for storing Reference 2 pipettes The pipette carousel features 6 holders that are designed for the Reference 2 Each holder features an image of a Reference 2 pipette to ensure that the pipettes are placed in the correct holder 2 5 2 Holder The holder can also be installed in the pipette carousel 3115 for Research plus pipettes 2 5 3 Wall mount An adhesive strip is used to prepare the wall mount for assembly on a level surface glass tile Safety Eppendorf Reference9 2 13 English EN 3 Safety 3 1 Intended use o The intended use is describ
7. 200 uL Blue 0 6 1 2 0 2 0 4 250 uL BO BE TEQN 40 6 41 5 40 2 40 5 71 mm 500 uL 0 6 3 0 0 2 1 0 1000 uL 0 6 6 0 0 2 2 0 Fixed volume Reference 2 Model 2 0 mL 2 5 mL Test tip epT I P S color code Volume range Length Red 0 5 mL 2 5 mL 115 mm Eppendorf AG error limits Error Systematic error Random error Uo mL 0 6 0 012 0 6 0 015 0 2 0 2 mL 0 004 0 005 36 Technical data Eppendorf Reference 2 English EN 7 4 Test conditions Test conditions and test evaluation match in compliance with ISO 8655 Part 6 Fine balance with evaporation protection inspected by the office of weights and measures Number of determinations 10 Use of water in accordance with ISO 3696 e Inspection at 20 C 27 C 0 5 C Dispensing on the tube wall 7 5 Ambient conditions Temperature range Relative humidity Storage without transport 5 C 45 C 10 9o 95 packaging Operating conditions 5 C 40 C 10 9o 95 Technical specifications subject to change Ordering Information Eppendorf Reference 2 English EN 8 Ordering Information 8 1 Single channel pipettes variable volume Order no Order no Description International North America Eppendorf Reference 2 variable Single channel serial number on upper and lower part 4920 000 016 4920000016 dark gray
8. a Reference 2 can be permanently changed by altering the factory adjustment Changes to the user or factory adjustment do not affect the dispensing precision random error Precision can be improved by exchanging worn parts Precision is also significantly affected by handling Maintenance Eppendorf Reference 2 29 English EN The existing dispensing volume must be checked before changing the factory calibration or adjustment The actual volume can be checked by weighing Mean value of the weighings Actual volume LL Density liquids at weighing temperature The density of distilled water is approx 0 9982 mg uL at 20 C and 0 9965 mgjuL at 27 C If the set volume is the same as the actual volume no correction is necessary If there is a difference between the actual volume and the set volume of distilled water please check the following Is there any liquid dripping from the tip Is the pipette tip fitted leak proof Is the tip cone undamaged Are the piston and cylinder leak proof Adequate leak tightness is ensured when no drop forms on the pipette tip after aspiration of the nominal volume with distilled water and a waiting time of approx 15 s Hold the pipette vertically without touching the pipette tip Prewet the tip several times for nominal volumes x 20 uL Does the temperature of the pipetted liquid correspond to The temperature of the device The ambient air temperature
9. due to penetration of liquids Do not put the pipette down when the pipette tip is filled Do not allow any liquids to penetrate the inside of the housing If liquid has entered the inside of the housing the inner parts may only be repaired by Eppendorf AG service partners Contact your local sales office before returning any devices 4 Operation Operation Eppendorf Reference 2 15 English EN 4 1 Setting the volume only Reference 2 variable 4 2 Using pipette tips gt To set the volume hold the volume locking button pressed down and turn the control button We recommend adjusting the volume setting from a higher value to a lower value If required turn beyond the required value and then back again The height of the control button changes as the volume is adjusted The volume is displayed up to 1000 uL in uL For 2 mL to 10 mL pipettes the volume is displayed in mL The liquid to be dispensed is aspirated into pipette tips We recommend using epT I P S epT I P S pipette tips which are available with various degrees of purity with and without filters and as special tips We recommend using special epT P S LoRetention tips with liquids that have a lower surface tension than water e g due to a wetting agent in the liquid Pipette tips are for single use only The pipette is only functional if a pipette tip has been attached You can either attach the pipette tip
10. from the delivery package e Pin Red plastic adjustment seal ADJ Insert the pin in the middle of the gray adjustment seal ADJ Remove the adjustment seal Insert the adjustment tool Turn the adjustment tool until the adjustment display shows the desired value g FB WN gt Read the set value distortion free using the aligning aid in the viewing window 6 Carry out weighings to check the accuracy and precision 7 Attach the red adjustment seal after the inspections If the adjustment is meant for a specific liquid mark the pipette accordingly Use the labeling area on the pipette for this and write down the liquid and the volume Carry out a gravimetric test for each adjustment change Follow the EN ISO 8655 2 and EN ISO 8655 6 test procedures A Standard Operating Procedure SOP and further information on user and factory adjustment settings can be found on the Reference 2 CD and on our website www eppendorf com Maintenance Eppendorf Reference9 2 31 English EN 6 5 3 Changing the factory adjustment Auxiliary aids from the delivery package Red plastic safety plug Pin The factory adjustment can be changed using the corresponding accessories The factory adjustment will be marked by a safety plug The color of the safety plug indicates the implementing authority Gray Eppendorf AG Red User The random and systematic errors recorded on delivery can be foun
11. oe 9 2 3 1 Reference 2 single chantel l i ute ee ER ER e es 9 2 3 2 Reference 2 Multi channel KK KK KK KK e 10 2 4 Material sia sh RII oh De CD bok A 0N pea WI SEPT ES 11 2 5 Storing the pipette erator bret ord lal eux Xaw exu a EE ee 12 2 5 1 Pipette carOusel o e henge taba Wire taba gee eae tere AR ois HEN 12 2 5 22 Holder co oo bL Doe ARO A IT e cep dox der k t ok e ER eges 12 2 5 3 Wallmount end shi dd Goa 27A tele Gd 21 23C2 wan h ee 12 Nri C E 13 3 1 Intended Usei 3 s noL kt LAE n ERE rua eine ceres eoe csi bs 13 3 2 Warnings for intended use sc 254 kai J202 X2 NR EAR AM E IR ER ELI 13 cun e Dabs 15 4 1 Setting the volume only Reference 2 variable aaa 15 4 2 Using pipette tips 24 det S y vests Ge Wa d nn IRURE Ue b 15 4 3 Deactivating the tip cone spring loading aa 16 4 4 Aspirating liquida 1 Xw WA A A ER RR I 9 Re hr 16 4 5 Dispensing liquid xvi Boni dere kulak da eR d ka ok s e Tsa ke a HR era cake BUR s OR eta RR 16 Troublesho ting rev ots ise est Ee S ea kin e meka keke ei rak eske ee nak es n ELE 17 5 1 Error searches N e I M S xe e eR lice tie ta dep taie ets 17 Mainit nan Ce 24 eene rrahe LER LIC 19 6 1 Cleaning i vienes Rue Rie ERAI e RR e A Zey bi PE 19 6 2 Sterilizing or disinfecting the pipette KK KK KK KK KK KK KK I Ih 20 QZ xAutoclavingss css evi d e ao eC kak UE U
12. or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment gt Only use accessories and original spare parts recommended by Eppendorf Safety 14 Eppendorf Reference 2 English EN NOTICE Damage to device from missing pipette tips Use the Reference 2 with fitted pipette tips only When using pipette tips without filters use 2 10 mL pipettes with protective sleeves and an inserted protection filter use of pipette tips The pipette tips are for single use only Prolonged use can have a negative impact on dispensing tasks NOTICE Carry over contamination and incorrect dispensing results due to the incorrect Only use the pipette tips once gt Do not use autoclaved ep Dualfilter T I P S for dispensing liquids Temperature differences between the pipette pipette tips and liquid or solutions with physical data that is very different from that of water may result in incorrect dispensing volumes NOTICE Incorrect dispensing volume due to differences in temperature and for special gt Avoid temperature differences the between pipette pipette tip and liquid gt Ensure that the temperature is between 20 C and 27 C and remains constant at 0 5 C gt Check the dispensing volume and make sure that you can affirm all the questions listed in the general information NOTICE Damage to the device
13. to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage 1 3 Symbols used Representation Meaning gt Handling 1 Actions in the specified order 2 List Direction of movement Text Name of fields in the software 0 Useful information 6 Operating instructions Eppendorf Reference 2 English EN Product description Eppendorf Reference 2 English EN 2 Product description 2 1 Features Reference 2 pipettes are piston stroke pipettes which operate according to the air cushion principle For air cushion pipettes a suitable pipette tip must be fitted prior to use The user controls the dispensing volume setting and ejector using the control button When the control button is pressed the piston in the pipette moves in the same direction If the control button and the piston are moved upward the liquid can be aspirated into the pipette tip When the piston is moved downward the liquid is dispensed measuring stroke Blow out occurs if the control button is pressed down past the first stop If the control button is pressed down again the pipette tip will be ejected The tip cone will spring load when a pipette tip is attached exceptions 2 mL to 10 mL pipettes The Reference 2 pipettes can be adjusted A viewing window below the adjustment seal indicates that a user adjustment has been modified The use
14. wey d l Wu aed m WW gd 36 8 Ordering Information leve s k lake iw e aye RE ER e Rem Pe Rigel ele ed eee 37 8 1 Single channel pipettes variable volume 37 8 2 Single channel pipettes fixed volume kk KK KK KK KK KK KK KK KK KK KI KK KK KK KK 37 8 3 Single channel lower part for variable volume and fixed volume KK 38 8 4 Multi channel pipettes kk kk kK kK Rh I hn 38 8 5 Multi channel lower parts kk kk KK KK KK KK KK KK KK KK KK eh 39 8 5 1 8 channel lower parts ai mecs cR Rep l RET 39 8 5 2 12 channel lower parts 2 44 Ku W AW K W An e nee 39 8 6 Spare parts accessories and pipette tips KK KK KK KK KK KK KK KK KK KK KK KK KK KK 39 94 PAt n S sh ks DD rne eie eq ees do n s terque iih os A CI e 41 Operating instructions Eppendorf Reference 2 English EN 1 Operating instructions 1 1 Using this manual Read this operating manual completely before using the device for the first time Please also note the operating instructions for the accessories if applicable This operating manual is part of the product Thus it must always be easily accessible Enclose this operating manual when transferring the device to third parties 1 2 Danger symbols and danger levels The safety instructions in this manual appear with the following danger symbols and danger levels 1 2 1 Danger symbols 1 2 2 Danger levels DANGER Will lead
15. 0 1 2 5 uL 4920 000 024 4920000024 medium gray 0 5 10 uL 4920 000 032 4920000032 light gray 2 20 uL 4920 000 040 4920000040 yellow 2 20 uL 4920 000 059 4920000059 yellow 10 100 uL 4920 000 067 4920000067 yellow 20 200 uL 4920 000 075 4920000075 orange 30 300 uL 4920 000 083 4920000083 blue 100 1 000 uL 4920 000 091 4920000091 red 0 25 2 5 mL 4920 000 105 4920000105 violet 0 5 5 mL 4920 000 113 4920000113 turquoise 1 10 mL 8 2 Single channel pipettes fixed volume Order no Order no Description International 4921 000 010 4921 000 028 4921 000 036 4921 000 044 4921 000 052 4921 000 060 4921 000 079 4921 000 087 4921 000 095 4921 000 109 4921 000 117 4921 000 125 4921 000 133 4921 000 141 4921 000 150 4921 000 168 4921 000 176 North America 4921000010 4921000028 4921000036 4921000044 4921000052 4921000060 4921000079 4921000087 4921000095 4921000109 4921000117 4921000125 4921000133 4921000141 4921000150 4921000168 4921000176 Eppendorf Reference 2 fixed Single channel serial number on upper and lower part dark gray 1 uL dark gray 2 uL medium gray 5 uL medium gray 10 uL yellow 10 uL light gray 20 uL yellow 20 uL yellow 25 uL yellow 50 uL yellow 100 uL yellow 200 uL blue 200 uL blue 250 uL blue 500 uL blue 1000 uL red 2 mL red 2 5 mL 37 38 Ordering Information Eppendorf Reference 2 English EN 8 3
16. 0 15 40 015 2 Reference 2 Multi channel variable Reference 2 Variable multi channel 8 channel 12 channel Model Test tip Testing volume Eppendorf AG error limits epT I P S color code Error Volume range Length Systematic error Random error uL uL 0 5 uL 10 uL Medium gray 0 5 uL 12 0 0 06 8 0 0 04 OBE Seo HE 1 jL 48 0 20 08 55 0 0 05 40 mm 5 uL 4 0 0 2 2 0 0 1 10 uL 2 0 0 2 1 0 0 1 10 uL 100 uL Yellow 10 uL 3 0 0 3 2 0 0 2 2 PES GIR ME 50 uL 1 0 1x05 1408 0 4 53 mm 100 uL 0 8 0 8 0 3 0 3 30 uL 300 uL Orange 30 uL 3 0 0 9 1 0 0 3 SUME SUME 150 uL 410 15 40 5 4075 55 mm 300 uL 0 6 1 8 0 3 0 9 7 3 Reference 2 fixed Technical data Eppendorf Reference 2 35 English EN Reference 2 fixed volume Model Test tip Eppendorf AG error limits epT I P S color code Error Volume range Length Systematic error Random error uL uL 1 uL Dark gray 2 5 0 025 1 8 0 018 2 uL pd 42 0 40 04 141 2 50 024 34 mm 5 uL Medium gray 1 2 0 06 0 6 0 03 10 uL wC Ms ep 1 0 0 1 0 5 0 05 40 mm 20 uL Light gray 0 8 0 16 0 3 0 06 0 5 jL 20 jL L 46 mm 10 uL Yellow 1 2 0 12 0 6 0 06 20 uL ERE E 1 0 0 2 0 3 0 06 53 mm 25 uL 1 0 0 25 0 3 0 075 50 uL 0 7 0 35 0 3 0 15 100 uL 0 6 0 6 0 2 0 2 200 uL 0 6 1 2 0 2 0 4
17. Is the weighing location free of drafts Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid Has the correct numerical value for Density liquids at weighing temperature been used to calculate the actual volume s the set volume correct e For very small volumes lt 10 uL Is the fine balance sufficiently sensitive balance resolution 0 001 mg e Were original epT I P S pipette tips used for testing Information on which pipette tip was used as the test tip can be found in the Technical data see p 33 No adjustment changes are allowed unless you can answer all the questions with yes In all other cases the problems associated with the questions answered with no must be eliminated If the problem is remedied by exchanging a complete lower part or other parts that affect the volume proper assembly must be verified by carrying out a gravimetric test Information on the systematic and random errors to be met can be found in the Technical data chapter 30 Maintenance Eppendorf Reference 2 English EN 6 5 2 Changing the user adjustment If the adjustment is changed the volume changes by a certain value Strictly speaking the change only applies to the testing volume Example You readjust a 10 100 UL pipette with a volume setting of 100 uL by 1 uL 1 uL 1 96 If the volume setting is 10 uL the pipette is also adjusted by 1 uL 10 Vo Auxiliary aids
18. NA RNA decontamination agents or 70 isopropanol You can wipe the outside of the tip cone and the ejector sleeve using a sodium hypochlorite solution of 4 After the exposure time of the sodium hypochlorite solution 15 minutes has elapsed thoroughly remove it using demineralized water The Reference 2 pipettes may be temporarily subjected to the UV light of a sterile bench 254 nm 6 3 Replacing O rings The 100 uL and 300 uL multi channel lower parts are equipped with O rings These are wear parts Replace old worn or damaged O rings Defective O rings result in the incorrect positioning of the tips and in dispensing errors 6 3 1 Removing the O ring 1 Switch off the spring loading 2 Push the opening of the O ring tool included in the delivery package against the tip cone from the side so that the sharp edge in the opening of the O ring tool is positioned on top of the o ring Do not slide the O ring tool onto the cone 3 Supporting the O ring tool with your thumb push it firmly against the tip cone This O ring is cut in one place 4 Remove the O ring tool and the O ring from the tip cone 6 3 2 Mounting a new O ring 1 Fit the mounting aid shortened pipette tip included in the delivery package of the O rings on the tip cone 2 Push the new O ring over the tip onto the tip cone 3 Check that the pipette tips are correctly positioned Ensure that the tips are tightly sealed and p
19. R e RO M RCM A e S wed ed hk RU 20 6 2 2 Disinfecti ni gt a Sr xu ct e e eL e PEL RES 21 6 3 Replacing O rings deri a a ER ue pau ian Sa 21 6 3 1 Removing the OSrin GJA 2 dd e Er Md e de e its 21 63 2 Mounting anew O rlhQ cs ede ees dd e RAS 21 6 4 Disassembling and assembling the pipette lower part aaa 22 6 4 1 Single channel lower part up to 1000 pl eee 22 6 4 2 Single channel lower part sse In 24 6 4 3 Multi channel lower part kk KK KK KK KK KK KK KK KK I 9h 26 Table of contents Eppendorf Reference 2 English EN 6 5 SAdjusting plDettes sc sa oo eee ek n Bae Teh a ae Da ele up wi We HE Eee RE e dib 28 6 5 1 General instructions on user and factory adjustment 000 28 6 5 2 Changing the user adjustment kK KK KK KK KK KK KK KK KK KK KK KK KK KK KK KH 30 6 5 3 Changing the factory adjustment KK KK KK KK KK KK KK e 31 6 6 Decontamination before shipment KK KK KK KK KK KK KK KK KK KK KK KK KK KK 32 A Technical datan isa nen ene a e ke a nanek ea et BENA ete det dedere 33 7 1 Reference 2 Single channel variable 33 7 2 Reference 2 Multi channel variable KK KK KK KK KK KK KK KK KK KK KK KK KK KK KK KK 34 7 3 Referenc 2 fiXeQ e ccu kl Pan e PY eser Q lee WA ar eh p en es 35 7 4 Testconditlons eco he r eB ERES Egr tS IR re Re ere URS ICE Ie easel 36 7 5 Ambientcoridilions 2 cas oy cee est ed heo dun de P V K
20. Remove any contamination from the exterior and the lower part gt If you remove existing grease slightly re lubricate the piston seal using only the grease specified in the ordering information see the enclosed CD 6 2 1 2 Procedure Prerequisites Follow the operating manual of the autoclave manufacturer Autoclave at 121 C 20 minutes 1 bar overpressure 1 When placing the Reference 2 into the autoclave make sure that the temperature at the pipette does not exceed 121 C 2 You can put the Reference 2 into the autoclave as a complete unit or with the lower part removed Do not disassemble the lower part 3 For2 mL to 10 mL pipettes remove old protection filter Add a filter sleeve and new protection filter and insert them after autoclaving Only autoclave the protection filter once 4 f lower parts have been removed make sure that no lower parts are confused during reassembly Tip use one plastic beaker per pipette 6 2 1 3 After autoclaving Cool the pipette to room temperature and let it dry For 2 mL to 10 mL pipettes press the protection filter into the filter sleeve and insert it into the tip cone The piston does not need to be relubricated after autoclaving If autoclaving is carried out as described above no gravimetric testing or adjustment is necessary Maintenance Eppendorf Reference 2 21 English EN 6 2 2 Disinfection gt Carefully wipe off the outer surfaces with disinfectant D
21. Single channel lower part for variable volume and fixed volume Order no Order no Description International 4920 601 001 4920 602 008 4920 603 004 4920 604 000 4920 605 007 4920 606 003 4920 607 000 4920 608 006 4920 609 002 4920 610 000 4920 611 007 North America 4920601001 4920602008 4920603004 4920604000 4920605007 4920606003 4920607000 4920608006 4920609002 4920610000 4920611007 Eppendorf Reference 2 lower part Single channel lower part with separate serial number 2 5 uL dark gray variable 0 1 uL 2 5 pL fixed 1 uL 2 uL 10 uL medium gray variable 0 5 uL 10 uL fixed 5 uL 10 uL 20 LL light gray variable 2 uL 20 uL fixed 20 uL 20 LL yellow variable 2 uL 20 pL fixed 10 uL 20 uL 100 LL yellow variable 10 100 pL fixed 25 uL 50 uL 100 uL 200 LL yellow variable 20 200 LL fixed 200 uL 300 LL orange variable 30 300 uL 1 000 LL blue variable 100 1 000 pL fixed 200 uL 250 uL 500 uL 1 000 uL 2 5 mL red variable 0 25 2 5 mL fixed 2 mL 2 5 mL 5 mL violet variable 0 5 5 mL 10 mL turquoise variable 1 10 mL 8 4 Multi channel pipettes Order no International 4922 000 013 4922 000 030 4922 000 056 4922 000 021 4922 000 048 4922 000 064 Order no North America 4922000013 4922000030 4922000056 4922000021 4922000048 4922000064 Description Eppendorf Reference 2 variable 8 channel serial numb
22. art from the upper part so that it can be removed 3 Put down the lower part with the lever facing downwards 4 Slide the two latches right and left at the side up The lower part is still lying on the table with the lever facing downwards 5 Remove the cover plate facing upward and the integrated ejector rail Do not remove the ejector rail from the cover plate The spring for the ejector rail may accidentally come off and get lost during this step Maintenance Eppendorf Reference 2 English EN 6 4 3 2 Removing and installing channels B ES Se 8l and 9 Position a pipette tip under the piston and carefully remove the piston from the upper rail 8 8 Carefully pull the piston out in an upward direction Do not bend the piston Hold the tip cone at the lower end and push it upwards slightly This compresses
23. by hand or directly insert the end of the pipette into a tip held in the tip storage box If attaching a pipette tip by hand it must be handled in a way that prevents contamination and heating of the pipette tip The color code on the Reference 2 control button matches the color of the epT I P S trays If you use pipette tips without filter insert the protection filter in the 2 mL to 10 mL pipette gt Fit the suitable pipette tip s on the tip cone applying light pressure The pipette tip sits firmly on the tip cone when it spring loads exceptions no spring loading for 2 mL to 10 mL single channel pipettes 16 Operation Eppendorf Reference 2 English EN 4 3 Deactivating the tip cone spring loading For the following dispensing tasks it may be helpful to deactivate the spring loading action of the tip cone A pipette tip is to be used over a long period of time The pipette tip is slightly bent during dispensing For pipette tips that are not designed for the tip cone of Eppendorf pipettes The spring loading action of the tip cone on single channel pipettes can be blocked by installing a locking ring On multi channel pipettes the spring loading action of the tip cone can be switched on or off using the selection switch 4 4 Aspirating liquid 1 The liquid to be aspirated must be taken from a suitable vessel For multi channel t pipettes we recommend the reagent reservoir Tip Tub 2 Press down t
24. d in the Eppendorf Certificate This certificate is included in delivery The certificate is no longer valid if the default settings have been changed If the dispensing accuracy is only be to changed temporarily the correct method is to change the user adjustment Before changing the user or factory adjustment observe the general notes see General instructions on user and factory adjustment on p 28 and the associated gravimetric tests The execution of changes to the factory adjustment is described on the Reference 2 CD The parts required to complete the change are included in the delivery package and are listed in the ordering information on the Reference 2 CD Maintenance Eppendorf Reference 2 English EN 6 6 Decontamination before shipment If you wish to return the device to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired please note the following CAUTION Use of a contaminated device may result in personal injuries and damage to A the device Clean and decontaminate the Reference 2 in accordance with the cleaning instructions before shipping or storage Hazardous substances are solutions presenting a hazard to health potentially infectious agents organic solvents and reagents radioactive substances proteins presenting a hazard to health DNA 1 Please note the information in the document Decontamination certificate for product returns You can find it as a PDF fil
25. e on our webpage www eppendorf com The decontamination certificate can also be found on the enclosed CD 2 Enter the serial number of the Reference 2in the decontamination certificate 3 With the shipment please include the completed decontamination certificate for product returns with the Reference 2 4 Send the pipette to Eppendorf AG or an Eppendorf AG service partner The address details of Eppendorf AG can be found on the last page of this operating manual The list of service partners can be found at www eppendorf com worldwide 7 Technical data 7 1 Reference 2 single channel variable Reference 2 Single channel variable Technical data Eppendorf Reference 2 English EN Model Test tip Testing volume Eppendorf AG error limits epT I P S color code Error Volume range Length Systematic error Random error uL uL 0 1 uL 2 5 uL Dark gray 0 1 uL 48 0 0 048 12 0 0 012 Increment 0 1 uL 10 uL 0 002 uL Sacer 0 25 uL 12 0 0 03 6 0 0 015 1 25 uL 2 5 0 031 1 5 0 019 2 5 uL 1 4 0 035 0 7 0 018 0 5 uL 10 uL Medium gray 0 5 uL 8 0 0 04 5 0 0 0025 Increment 0 1 uL 20 uL 0 01 uL AO min 1 uL 2 5 0 025 1 8 0 018 5 uL 1 5 0 075 0 8 0 04 10 uL 1 0 0 10 0 4 0 04 2 uL 20 uL Light gray 2 uL 5 0 0 10 1 5 0 03 Increment 0 5 uL 20 uL L 0 02 uL aem 10 uL 1 2 0 12 0 6 0 06 20 uL 1 0 0 20 0 3 0 06
26. e piston mount so that the two locking mechanisms engage in their retainers Gently press a pipette tip against the inserted piston The piston must move down in the cylinder without any noticeable resistance Installation of the lower part and ejector sleeve 4 5 Insert the lower part into the upper part until it audibly engages Set the maximum volume and operate the control button several times It must run smoothly and resistance free Keep the ejector pressed The ejector rod protrudes from the upper part Place the ejector sleeve on the ejector rod If correctly fitted it will engage slightly Carry out a gravimetric test of the systematic and random error This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 24 Maintenance Eppendorf Reference 2 English EN 6 4 2 Single channel lower part Applies to the following single channel pipettes 2mL 2 5 mL 5mL 10 mL Replace the protection filter in the tip cone each time it comes into contact with liquid 6 4 2 1 Exchanging the filter Y 1 Remove the filter sleeve 2 Use a pipette tip to push out the old filter 3 Insert a new filter into the filter sleeve Maintenance Eppendorf Reference 2 25 English EN 4 Insert the filter sleeve aps e eppendorf Referer o U 1 Unscrew the ejector sleeve
27. ed in a separate document enclosed in the package 3 2 Warnings for intended use A WARNING Damages to health due to infectious liquids and pathogenic germs When handling infectious liquids and pathogenic germs observe the national regulations the biological security level of your laboratory the material safety data sheets and the manufacturer s application notes Wear personal protective equipment Consult the Laboratory Biosafety Manual Source World Health Organization Laboratory Biosafety Manual as amended for comprehensive regulations on the handling of risk group II germs or biological materials IN WARNING Damage to health due to toxic radioactive or aggressive chemicals Wear personal protective equipment Observe the national regulations for handling these substances Observe the material safety data sheets and manufacturer s application notes AN CAUTIONI Danger to individuals due to careless use Never point the opening of a Reference 2 which is equipped with a pipette tip at yourself or other persons Only initiate dispensing if it is safe to do so With any dispensing task please ensure that you do not endanger yourself and other persons CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable
28. eppendorf Register your instrument www eppendorf com myeppendorf Operating manual Copyright 2013 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner Trademarks Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG Hamburg Germany Combitips epT I P S 9 and Reference 2 are registered trademarks of Eppendorf AG Hamburg Germany Registered trademarks are not marked in all cases with 7M or 9 in this manual Further information can be found on the enclosed CD 4920 900 017 01 052013 Table of contents Eppendorf Reference 2 English EN Table of contents 1 Operating instructions oor dina be ex ane nekena ER ARES RENE RA DERE 5 1 1 Usingsthis manualz xana niner Kn Qu ya rO Kay re RE CU REPE QR cae REPRE SLE 5 1 2 Danger symbols and danger levels KK KK KK KK KK KK KK eh 5 1 2 1 DanGer SyiMbol Si sure Du ada y DE t d cde tae er be ER de e Kee Ree u dul en E EYE ed 5 LZZ Danger levelsz sep dp ad T Ae tt e at it ae 5 13 Symbols sed ine ve E vd 5 Produc description aici iota ler end e ev el a i t T e EE 7 2 1 w gt W7 gt gt wweweo _ mmmmmmmmeeeemm 7 2 1 1 Pipette models gt da a d Ra peal k Wa SN d e EIE EA EN Eden 7 2 2 Delivery PpaGkag di cj eco rote SOPE BOs BU RETSESU POP SOR E e VES T Ser eR Ge ES 8 2 3 Main lll stration cns erat pai KUDA eit v aen el de n E eee
29. er on upper and lower part medium gray 0 5 10 uL yellow 10 100 uL orange 30 300 uL Eppendorf Reference 2 variable 12 channel serial number on upper and lower part medium gray 0 5 10 uL yellow 10 100 uL orange 30 300 uL 8 5 Multi channel lower parts 8 5 1 8 channel lower parts Ordering Information Eppendorf Reference 2 39 English EN Order no International 4922 601 009 4922 603 001 4922 605 004 Order no North America 4922601009 4922603001 4922605004 Description Eppendorf Reference 2 lower part 8 channel lower part with separate serial number medium gray 0 5 10 uL yellow 10 100 uL orange 30 300 uL 8 5 2 12 channel lower parts Order no International Order no North America Description Eppendorf Reference 2 lower part 12 channel lower part with separate serial number 4922 602 005 4922602005 medium gray 0 5 10 uL 4922 604 008 4922604008 yellow 10 100 uL 4922 606 000 4922606000 orange 30 300 uL 8 6 Spare parts accessories and pipette tips o The ordering information can be found on the Reference 2 CD Ordering Information 40 Eppendorf Reference 2 English EN Patents Eppendorf Reference 2 41 English EN 9 Patents U S Patent No 7 434 484 Is used by Reference 2 pipettes 1 uL 2 uL 2 5 uL 5 uL 10 uL with variable volume setting 20 uL 25 uL 50 uL 100 uL single channel and multi channe
30. hannel lower part 27 S Spring mechanism eese 22 U USING Pipette tips sesa meet 15 Evaluate your manual Give us your feedback www eppendorf com manualfeedback Your local distributor www eppendorf com contact Eppendorf AG 22331 Hamburg Germany eppendorf eppendorf com www eppendorf com eppendorf
31. he control button to the first stop measuring stroke 3 Immerse the pipette tip s vertically approx 4 mm into the liquid 4 Toaspirate liquid allow the control button to slide back slowly Maintain the immersion depth to ensure no air is aspirated 5 n the case of large volumes before removing the pipette tip from the liquid wait for approx 3 seconds To ensure maximum precision and accuracy we recommend to wet each new tip initially by aspirating and dispensing the liquid one to three times Only then should pipetting commence 6 Remove the tip s slowly from the liquid 7 Wipe the tip s slowly against the tube wall to ensure that no outer wetting remains on the tip Dispensing liquid 4 5 1 Place the tip on the tube inner wall at an angle j 2 Press the control button slowly until the first stop measuring stroke and wait until the flow of liquid stops Hold down the control button and wipe the tip on the tube inner wall To eject the tips hold the control button all the way down on I 3 To empty the tip press the control button until the second stop blow out 5 Troubleshooting 5 1 Error search Symptom message Liquid is dripping from the tip and or the dispensed volume is incorrect Cause The tip is loose or the pipette tip is poorly fitted Troubleshooting Eppendorf Reference9 2 English EN 17 Remedy gt Firmly attach the pipette tips Use ept 1 P S pipette tips
32. l 200 uL 250 uL 300 uL 500 uL and fixed volume with following and 1000 uL nominal volumes U S Patent No 7 673 532 Is used by Reference 2 pipettes 10 uL 100 uL 300 uL with variable volume setting multi channel with following nominal volumes U S Patent No 7 674 432 Is used by Reference 2 pipettes 1 uL 2 uL 2 5 uL 5 uL 10 uL with variable volume setting 20 uL 25 uL 50 uL 100 uL single channel and multi channel 200 uL 250 uL 300 uL 500 uL and fixed volume with following 1000 uL 1 mL 2 mL 2 5 mL 5 mL nominal volumes and 10 mL U S Patent No 8 297 134 is used by Reference 2 pipettes 1 uL 2 uL 2 5 uL 5 uL 10 uL with variable volume setting single channel and multi channel and fixed volume with following nominal volumes 20 uL 25 uL 50 uL 100 LL 200 uL 250 uL 300 uL 500 uL 1000 uL 1 mL 2 mL 2 5 mL 5 mL and 10 mL 42 Index Eppendorf Reference 2 English EN Index A Assembly 10 uL 100 uL and 300 uL multi channel lower erg RT 27 Single channel lower part sess 24 Single channel lower part up to 1 000 pL 23 C Cleaning eet eterni eee die 19 D Disassembly 10 uL 100 uL and 300 uL multi channel lower PAK ND DD 26 Single channel lower part eee 24 Single channel lower part up to 1 000 pL 22 L LOCKING ring ris ss yr give 22 O O ring ar EEEE NR ERES 21 21 R Replace channel 10 uL up to 300 uL multi c
33. mpatibility with the materials used Please note the information on chemical resistance see CD Do not clean the Reference 2 with acetone or organic solvents with a similar effect Do not clean the Reference 2 with sharp objects v v cv v v Remove external contamination on the Reference 2 as follows gt Weta cloth with a mild cleaning fluid and water and remove the contamination To remove heavy contamination resulting from liquid penetration disassemble the lower part of the pipette and clean it with demineralized water Relubricate the piston sealing rings after contamination use of aggressive chemicals and or heavy stress Remove the old grease before relubricating Only use the grease specified in the ordering information see the enclosed CD 20 Maintenance Eppendorf Reference 2 English EN 6 2 Sterilizing or disinfecting the pipette NOTICE Damage to device from incorrect handling Do not use any additional disinfectants decontamination agents or sodium hypochlorite during autoclaving or UV exposure When autoclaving please ensure that the temperature does not exceed 121 C Before using disinfecting agents or decontaminating agents test for material compatibility and check the manufacturer s instructions on chemical resistance Please also note the pipette material 6 2 1 Autoclaving All Reference 2 pipettes are completely steam autoclavable 6 2 1 1 Before autoclaving gt
34. on Maintenance Eppendorf Reference 2 23 English EN 6 4 1 3 Opening and assembling the lower part lt 1000 uL 0 To avoid confusion of parts only disassemble and assemble one pipette at a time J Q a 3 nz PUSH UPTO RELEASE PUSH UPTO RELEASE im A Opening the lower part 1 2 3 Push the stop pins together slightly on the piston mount 1 Remove the piston mount Remove the piston 2 and the piston spring 3 The piston is under spring tension The appearance of pistons and piston springs will vary for the individual volume versions Assembling the lower part 1 Carefully guide the piston and the piston spring into the cylinder Make sure that the piston is guided correctly in the piston spring and the cylinder There must not be any perceptible resistance Stop pushing as soon as any resistance is felt This may indicate that the piston is not positioned correctly in the cylinder There is a risk of bending the piston if too much force is applied Carefully pull out the piston and repeat the process correctly In the case of piston springs with double windings 4 these windings must point downwards Keep the piston and the piston spring pressed Keep the stop pins on the piston mount pressed with the other hand Mount th
35. ppendorf Reference 2 gas 7 10 11 Product description Eppendorf Reference 2 English EN 1 Volume display Volume display with four digit counter for variable pipettes Decimal places are marked by a hyphen 2 Color code The color code on the control button and the trays of the matching epT I P S pipette tips are in the same color 3 Control button Sets and dispenses the volume and ejects the pipette tip 4 Volume locking button Locks the control button volume setting ring Press a volume locking button at the left or right to set the volume 5 User adjustment Upon delivery the opening is sealed using a gray plastic adjustment seal ADJ 6 User adjustment display Set to 0 before delivery 7 Serial number Serial number for upper and lower parts 8 Nominal volume 9 Ejector sleeve Ejects the pipette tip Press the control button down all the way 10 Spring loaded tip cone The spring loading action optimizes the force required to attach and eject tips no spring loaded action for 2 mL to 10 mL For 2 mL to 10 mL pipettes an exchangeable protection filter in a sleeve is located in the tip cone 11 Pipette tip We recommend using epT l P S Product description Eppendorf Reference 2 English EN 2 3 2 Reference 2 Multi channel For an explanation of the upper part of the pipette refer to the main illustration of the single channel pipette
36. r adjustment functions independent of the factory adjustment All Reference 2 pipettes are fully autoclavable 2 1 1 Pipette models The Reference 2 family consists of single channel and multi channel pipettes 8 and 12 channels with variable volume settings and single channel fixed volume pipettes For Reference 2 pipettes with variable volume settings the volume can be changed by turning the control button when the volume locking button is pressed Volumes from 0 1 uL to 10 mL can be dispensed depending on the model A list of all available models is available in the Technical Data Product description Eppendorf Reference 2 English EN 2 2 Delivery package The following accessories are included in the delivery package Piece Description 1 Operating manual 1 Certificate 1 Allen key with a blue handle adjustment tool 1 CD with information on chemical resistance user adjustment factory adjustment and ordering information 1 Black locking ring for single channel pipettes x 1000 uL 5 Red plastic adjustment seal ADJ 5 Protection filter consisting of 1 filter sleeve and 10 filter cartridges for 2 mL to 10 mL pipettes 1 Pipette wrench for opening the lower part 2 mL to 10 mL 1 Multi channel tool for cutting the O rings only 100 uL and 300 uL multi channel lower parts 5 Red plastic safety plug 1 Pin 2 3 Main illustration 2 3 1 Reference 2 single channel Tf e
37. recorded on delivery can be found in the Eppendorf Certificate This certificate is included in delivery Changes to the factory adjustment will render the certificate void 6 5 1 General instructions on user and factory adjustment The Reference 2 was adjusted and tested prior to delivery The adjustment opening is sealed using a gray plastic adjustment seal The adjustment seal is labeled with the abbreviation ADJ The adjustment display shows 0 Changing the adjustment of the Reference 2 is sometimes recommended for solutions that are very different from water in terms of their density viscosity surface tension and or vapor pressure etc If the density of an aqueous solution changes by approximately 10 for example due to the salt concentration the volume changes by approximately 0 2 This statement does not apply if other relevant properties of the liquid also change If the pipette is used at extremely high altitudes it must be calibrated to the ambient air pressure At 1000 m above sea level the volume error of a 100 uL pipette is about 0 3 Vo When using special tips i e tips with a geometry that is very different from standard tips changing the adjustment can improve the dispensing accuracy systematic error Follow the adjustment tips for epT I P S long provided in the User adjustment document on the Reference 2 CD Adjustment changes can be reset using simple steps In addition to changing the user adjustment
38. roperly aligned 22 Maintenance Eppendorf Reference 2 English EN 6 4 Disassembling and assembling the pipette lower part 6 4 1 Single channel lower part up to 1000 uL 6 4 1 1 Removing the lower part PUSH TO RELEASE Hold the control button completely pressed down 2 Remove the ejector sleeve 3 and On the lower part slide the ring marked PUSH UP TO RELEASE up by about 5 mm until the lower part comes off 4 9 Remove the lower part from the upper part 6 4 1 2 Deactivating spring loading For the following dispensing tasks it may be helpful to deactivate the spring loading action of the tip cone A pipette tip is to be used over a long period of time The pipette tip is slightly bent during dispensing Deactivating this action may also be helpful for pipette tips that are not designed for the Eppendorf pipette tip cone 6 4 1 2 1 Single channel pipettes The spring loading action of the tip cone can be deactivated by installing the locking ring The locking ring is included in the delivery package 1 Push the black locking ring onto the lower part from the top by slightly squeezing the clamps on the lower part 2 Insert the lower part into the upper part until it audibly engages 3 Hold the control button completely pressed down 4 Mount the ejector sleeve If correctly fitted it will engage slightly Unlock the locking ring to reactivate the spring loading acti
39. the spring Lightly lift the tip cone and release it from the lower rail Relax the spring by letting the tip cone slide back above the lower rail Remove the tip cone along with the cylinder and spring from the upper rail Before installation slide the piston into the cylinder Install the channels in reverse order 6 4 3 3 Assembling the 10 300 uL multi channel pipette B UU Na a Fit the cover plate with the integrated ejector Push the latches downward To mount push the lower part into the upper part until it audibly engages Carry out a gravimetric test of the systematic and random error This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 27 28 Maintenance Eppendorf Reference 2 English EN 6 5 Adjusting pipettes liquids Temperature differences between the pipette pipette tips and liquid or solutions with physical data that is very different from that of water may result in incorrect dispensing volumes NOTICE Incorrect dispensing volume due to differences in temperature and for special Avoid temperature differences the between pipette pipette tip and liquid gt Ensure that the temperature is between 20 C and 27 C and remains constant at 0 5 C Check the dispensing volume and make sure that you can affirm all the questions listed in the general information p The random and systematic errors
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